TY - JOUR. T1 - Monoclonal antibody to a nucleolar antigen of human B-lymphoblastoid cells. AU - Todorov, I. T.. AU - Philipova, R. N.. AU - Zhelev, N. Z.. AU - Hadjiolov, A. A.. PY - 1987/3. Y1 - 1987/3. N2 - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of rRNA transcription with Actinomycin D caused redistribution of the 41 5.6 antigen, but even at long term drug action it remains associated with the nucleolar remnants.. AB - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of ...

Human immortalized keratinocyte cell line HaCaT. Cultures of HaCaT cells were kindly provided by Dr. Norbert E. Fusenig (Institute of Biochemistry, German Cancer Research Center) and were grown on DMEM supplemented with 1% L-glutamine, 25 mM HEPES, 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified incubator with 5% CO2 atmosphere (10, 14).. Assessment of cell morphological changes and viability. HaCaT cells at the density of 2×105 cells/well were placed onto 12-well plates and incubated at 37°C for 24 h before being treated with different concentrations of citric acid for 24 h; 0.5% of DMSO (vehicle) was used as a control. At the end of incubation, cells were examined and photographed under phase-contrast microscopy for determination of morphological changes. Cells (1×105 cells per sample) were then centrifuged at 1000 ×g for 5 min, and cell pellets were dissolved with 0.5 ml of Phosphate buffered saline (PBS) containing 5 μg/ml PI and viable cells were determined by using a ...

A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000- …

TY - JOUR. T1 - Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage-like cell line J774. AU - Szabõ, Rita. AU - Láng, Orsolya. AU - Láng, Júlia. AU - Illyés, Eszter. AU - Köhidai, Lászlõ. AU - Hudecz, Ferenc. PY - 2015/4. Y1 - 2015/4. N2 - WSXWS motif is a conserved amino acid sequence that is present in type I cytokine receptors. This motif that can be found both in the ligand binding chains and signal transducer molecule of the receptors with different amino acids at the position X plays a role in the receptor folding, ligand binding and signal transduction as well. Structural analysis proved that WSEWS motif of IL-6R is located in a highly accessible location in the protein. Structural properties and chemotaxis of a tetrapeptide library with SXWS sequence, where X was the 19 proteinogenic amino acids except cystein were systematically studied earlier. It has been proved that C-terminal amidation and the identity of amino acid X had a pronounced influence on ...

We have constructed a HeLa cell line that both expresses high levels of CD4 and contains a single integrated copy of a beta-galactosidase gene that is under the control of a truncated human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR). This cell line, called CD4-LTR/beta-gal, can be used to determine quantitatively the titer of laboratory-adapted HIV strains, and the method used to do so is as sensitive as the determination of viral titers in a T-cell line by end point dilution. Using this cell line as a titer system, we calculated that HIV-1 stocks contain only one infectious particle per 3,500 to 12,000 virions. Virus derived from a molecular clone of a macrophagetropic provirus will not infect this cell line. We have also cocultivated peripheral blood lymphocyte cultures from HIV-infected individuals with the CD4-LTR/beta-gal indicator cells. In a majority of primary isolates (five of eight), including isolates from asymptomatic patients, rare virus-infected cells that can ...

Saferali A, Grundberg E, Berlivet S, Beauchemin H, Morcos L, Polychronakos C, Pastinen T, Graham J, McNeney B, Naumova AK. Cell culture-induced aberrant methylation of the imprinted IG DMR in human lymphoblastoid cell lines. Epigenetics. 2010 Jan 01; 5(1):50-60 ...

TY - JOUR. T1 - Characterization of lymphoid cell lines from murine spleen which can localize in thymus. AU - ONeill, H C. AU - De Cello, G. AU - ONeill, T J. PY - 1992/2. Y1 - 1992/2. N2 - Several murine lymphoid cell lines have been tested for specific capacity to localize in thymus. These are all continuous, cloned Radiation leukemia virus-induced cell lines which have a common phenotype resembling lymphoid stem cells or immature T cells. Since each of these cell lines has a cloning efficiency approaching 100%, the number of cells which enters thymus during a 3 h homing assay has been estimated by limit dilution cloning analysis taking into account extra-binomial variation caused by individual mice. Only two out of seven of these cell lines have been found to have this specific property. These two cell lines, 16C1 and 5C2B, have been characterized as immature lymphoid cells, bearing no rearrangement at the TCR gamma and beta loci, and having the phenotype of CD3+CD4-CD8-, immature T cells. ...

Müller glial cultures have made a significant contribution for understanding retinal pathophysiology, especially in the context of retinal degenerative diseases. Primary Müller glial cultures have several advantages, largely due to their phenotypic and functional similarity to Müller glia in vivo. There are, however, several disadvantages in using primary cultures, including limited availability, and inconsistency in experimental results, perhaps due to purity, heterogeneity across different batches, and phenotypic instability across multiple passages.14 The use of a stabilized cell line can overcome these drawbacks, although it is recommended that key results obtained from cell lines are further validated using primary cells and in vivo models. Various Müller glial cell lines, derived from rodents14-17 or humans18 have been reported. Although they all express Müller glial markers, each cell line has its own characteristics and is used to study different functions of Müller glia. For ...

Hybrid (both intra-species and inter-species) cell lines arise through intentional or nonintentional fusion of somatic cells having different origins. Hybrid cell lines can pose a problem for authentication testing to confirm cell line identity, since the results obtained may not conform to the results expected for the two parental cell types. Thus, depending on the identity testing methodology, a hybrid cell may display characteristics of one of the parental cell type or of both. In some instances, the hybrid cell line may display characteristics that are different from those displayed by either parental cell type; these differences may not necessarily indicate cellular cross-contamination. Testing should be performed as soon as possible after an intended fusion has occurred, so that a baseline reference profile is available for later comparison. In this article, we describe the various approaches that have been used for identifying hybrid cell lines and the results that might be expected when using

Stable cell lines play an important role in the study of recombinant protein preparation, antibody preparation, drug screening, gene function and other aspects. The stable cell lines can continue to grow, and express foreign gene. Changing the copy number and promoter activity can regulate the expression level of exogenous gene in target cells, which makes it increase, decrease, or equal to the endogenous gene expression amount of target cells. Insert modes of exogenous genes can be divided into random insertion or fixed position insertion.. Creative Biogene is now providing stable cell lines construction service for the majority of researchers. We use the most advanced technology, to provide a stable cell line with high quality for you. The construction service can use CHO, HEK293, Hela, NIH3T3 and other tools, or use specific cells designed by customers. From cloning construction, cell line establishment, gene amplification, monoclonal screening, cell line identification, functional research, ...

However, it is not easy to develop a stable cell line that maintains ideal growth and productivity characteristics. There are many factors that can influence the bioproduction level including vector expression elements, vector delivery efficiency, screening system etc. This imposes very significant costs and time in the development of a desirable bioproduction stable cell line.. Leading Capability. As an unmatched global provider of life science services and products, Creative Biogene offers comprehensive services for generating bioproduction stable cell lines. Our service scope covers all steps of stable cell line generation, from the first gene synthesis to the final delivery of high-producing cell clones.. To improve the production rate of recombinant proteins and the selection efficiency, combined with our genome editing platform, Creative Biogene has developed our own mammalian cell expression systems including cells based on glutamine synthetase (GS) selection and cells based on ...

Velagapudi, Ravikanth, Olajide, Olumayokun A and Aderogba, Mutallib A. (2014) Tiliroside Produced Anti-Neuroinflammatory Effects Through Interference With NF-κB And MAPK Signalling In LPS+ IFN-γ Stimulated BV-2 Microglia. pA2 Online. ISSN 1741-1149 ...

De la chapelle, A; Fantoni, A; and Marks, P A., Differentiation of mammalian somatic cells, dna and hemoglobin synthesis in fetal mouse yolk sac erythroid cells. (1969). Subject Strain Bibliography 1969. 1375 ...

Fig. 1. Acentriolar cell lines establishment and characterization. (A) Different 2-3-week-old primary cultures obtained from dissociated embryos. Cells present various morphologies. Scale bar: 50 µm. (B) Representation of the survival of primary cultures. Most of primary cultures differentiate and stop to grow but some of them spontaneously immortalize. 11 permanent cell lines have been obtained. (C) FACS profiles of a young mixed line (line 23) before (blue) and after (green) heat shock. X-axis: intensity of fluorescence. Y-axis: number of cells. The narrowing of the fluorescence peak reflects the selection against hs-hid genotypes. (D) PCR genotyping of the 11 cell lines. Two couples of primers (AB or BC, see Materials and Methods) allow detection of WT or mutant DSas-4 alleles. From this analysis 6 lines are pure DSas-4−/− (as line 131) and 5 lines are DSas-4+/− (as line 110). (E) Immunofluorescence of control (upper image) and DSas-4−/− no. 70 (lower image) mitotic cells stained ...

HLA A*0201 WT 1 complex Protein Human HEK293 His best suppliers; HLA A*0201 WT 1 complex Protein Human HEK293 His best sources; HLA A*0201 WT 1 complex Protein Human HEK293 His best vendors; HLA A*0201 WT 1 complex Protein Human HEK293 His protocol; HLA A*0201 WT 1 complex Protein Human HEK293 His citations; HLA A*0201 WT 1 complex Protein Human HEK293 His publications; HLA A*0201 WT 1 complex Protein Human HEK293 His papers - Labshake

RNA interference is an evolutionary conserved immune response mechanism that can be used as a tool to provide novel insights into gene function and structure. The ability to efficiently deliver small interfering RNA to modulate gene expression in vivo may provide new therapeutic approaches to currently intractable diseases. In vitro, siRNA targeting IL-12p40 was delivered to the murine macrophage cell line (J774A.1) encapsulated in a liposome with an IL-12 inducing agent (LPS/IFN-γ) over a number of time points. Controls included a variety of non-target specific siRNA reagents. Supernatants were analyzed for cytokine production while the cells were removed for mRNA profiling. In vivo, siRNA-targeting IL-12p40 was delivered to the murine peritoneal cavity in a therapeutic fashion, after endotoxin (LPS) challenge. Cells from the peritoneal cavity were removed by lavage and analyzed by flow cytometry. Levels of IL-12 present in lavage and in serum were also examined by ELISA. In this report, we show that

In: Proceedings of a symposium on continuous cell lines as substrates for biologicals, Arlington, Virginia USA, May 1988. Basel: Karger, 1989;113-24 (Developments in biological standardization; 70 ...

Shirley, NY-Profacgen, a world-leading custom protein service provider for a broad spectrum of industrial and academic clients with a commitment to delivering high-quality data and customer services, is pleased to update its stable cell line construction services. Scientists in the field of protein expression now have access to Profacgens latest stable cell line construction service version supported by cutting-edge facilities.. When large amount of recombinant protein is required, stable expression line construction is recommended for reproducible and cost-effective protein production. Generally, stable cell line construct will take 3 steps: delivery of the target gene into the host cell, screening of stable expression clones, and stability test. For efficient gene delivery and to achieve high integration rate, the company uses lentiviral vector. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When ...

DC-SIGN cell surface distribution during monocyte-derived DC development. DC-SIGN binding activity was monitored during development of monocyte-derived DCs. As

The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.

TY - JOUR. T1 - Isolation of myoblastic, fibro-adipogenic, and fibroblastic clonal cell lines from a common precursor and study of their requirements for growth and differentiation. AU - Darmon, Michel. AU - Serrero, Ginette. AU - Rizzino, Angie. AU - Sato, Gordon. N1 - Funding Information: We thank Dr H. Jakob for the gift of the T984 cell line and for the stimulatingd iscussionswhich led to this study and also Drs H. E&en, J.-F. Nicolas, M. H. But and F. Jacob for stimulatingd iscussions. We thank Dr R. Bloch for his assistancei n performing experimentsw ith rhodamine-labeleda BT and Drs J. Orly, D. McClure, and D. Barnes for their valuable help during this work. We thank Dr D. Weinstein for helu in oreoarine delioidated and delioooroteinized sera and-D; S. Cohenfor the gift of high molecular weight EGF. These experimentsw ere conductedd ur-ingthe tenure of an ACS-Eleanor Roosevelt lnter- national Cancer Fellowship awarded by the Intema-tional Union Against Cancer to M. D. USPHS-CA 19731a nd ...

Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...

Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment wit...

TY - JOUR. T1 - Functional analysis of an established mouse vascular endothelial cell line. AU - Nishiyama, Tatsuaki. AU - Mishima, Kenji. AU - Ide, Fumio. AU - Yamada, Koichi. AU - Obara, Kumi. AU - Sato, Aki. AU - Hitosugi, Noriko. AU - Inoue, Hiroko. AU - Tsubota, Kazuo. AU - Saito, Ichiro. PY - 2007/2/1. Y1 - 2007/2/1. N2 - Background: In vitrostudies using cell lines are useful for the understanding of cellular mechanisms. The purpose of our study is to develop a new immortalized aortic vascular endothelial cell (EC) line that retains endothelial characteristics and can facilitate the study of ECs. Methods: A mouse aortic vascular EC line (MAEC) was established from p53-deficient mouse aorta and cultured for over 100 passages. The expression of endothelial markers was assessed, and the function of this cell line was analyzed by tube formation and binding assays. Results: MAEC retained many endothelial properties such as cobblestone appearance, contact-inhibited growth, active uptake of ...

Peculiar characteristic: the use of a single continuous line. One Continuous line. From Caracas, Venezuela, Ronald was born and raised in a family of painters and designers. His father was a graphic designer, sketching logos with him from a very young age, a profession that Ronald himself would also later pursue in parallel with his artistic practices. Ronald has a peculiar characteristic in his work: the use of a single continuous line. Each piece Ronald paints demands your attention, inviting you to trace the journey of the single line.. The line to me is life and life is a continuous, uninterrupted current events, also it corresponds to the idea that we are all connected, this is the idea of karma, everything we do somehow affects others and comes back to us ...

...ANN ARBOR Mich. The University of Michigans first human embryonic s...The line known as UM4-6 is a genetically normal line derived in Oct... This is significant because acceptance of these cells on the registr... It now makes the line available to researchers who can apply for fede...,U-M,human,embryonic,stem,cell,line,placed,on,national,registry,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters

Last week, we finished our Ask the Expert Discussion on Cell Line Authentication. Inadvertent cell line contamination is a serious concern for many researchers. Failure to monitor cell lines for contamination may result in compromised data. Current estimates are that 18% to 36% of all active cell lines are either cross-contaminated or misidentified.1

Endothelial cells are believed to play an important role in response to virus infection. Our previous microarray analysis showed that H9N2 virus infection and inactivated viral particle inoculation increased the expression of interferon-inducible transmembrane protein 1 (IFITM1) in human umbilical vein endothelial cells (HUVECs). In present study, we deeply investigated the expression patterns of IFITM1 and IFITM1-mediated antiviral response induced by H9N2 virus infection and inactivated viral particle inoculation in HUVECs. Epithelial cells that are considered target cells of the influenza virus were selected as a reference control. First, we quantified the expression levels of IFITM1 in HUVECs induced by H9N2 virus infection or viral particle inoculation using quantitative real-time PCR and western blot. Second, we observed whether hemagglutinin or neuraminidase affected IFITM1 expression in HUVECs. Finally, we investigated the effect of induced-IFITM1 on the antiviral state in HUVECs by siRNA and

The Infectious Disease Cell Line Microarray consists of either 2 or 9- 2mm cores of formalin-fixed paraffin-embedded infected cell lines, which were assembled in array fashion to allow multiplex molecular pathology analysis and validation of infectious disease reagents, or to be used as controls for Immunohistochemistry and/or in situ hybridization (CISH and FISH) applications.The 9-core Infectious Disease Cell Line Microarray consists of all positive (+) cell lines listed below, as well as one negative (-) core. The 2-core Infectious Disease Cell Line Microarrays include one positive (+) and one negative (-) core.. ...

Hello, I am working with HepG2-C3A hepatocyte cell line. My goal is to transfect these cells with GFP reporter plasmids and establish stable cell lines. The plasmids have Neomycin resistance gene in them. Can somebody please help me with these queries? 1. Does anybody have a set protocol that describes the procedure starting from trancfecting cells to performing gene expression studies using stably transfected cells? 2. Do the cells usually have a different generation time after stable transfection? 3. Do you have to screen for clones that behave as close as the wild type? If so, how many do you have to screen? 4. How long does this whole procedure take? Cheers, Murali Vemula ...

Hello all- I am in need of a good all around human endothelial cell line for use in viral transformation studies, as well as studies of certain viral genes. I have looked through some of the thousands at ATCC, but most have been derived from leukemias, etc. I would prefer one that is normal (as normal as a culturable cell line can be). The big restriction is that I am looking for an endothelial line. Thanks in advance. Kajetan -------------- next part -------------- A non-text attachment was scrubbed... Name: Kajetan-Groicher.vcf Type: text/x-vcard Size: 427 bytes Desc: Card for Kajetan H. Groicher Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19991110/240b4f7f/Kajetan-Groicher.bin ...

TGF-β isoforms are important signalling molecules in wound repair in the skin. Transforming growth factor β3 (TGF-β3) has been implicated in scarless healing. In both animal and human models the application of exogenous TGF-β3 causes a reduction in the inflammatory response and improves the architecture of the neodermis. Research into the influence of TGF-β on scarring has tended to focus on fibroblasts. However, keratinocytes play a major role in scarring both indirectly, as a result of their influence over the behaviour of fibroblasts and also by directly influencing wound contraction. Thus, experiments were carried out to investigate the influence of TGF-β3 on the behaviours of a keratinocyte cell line (HaCaT). Incubation with TGF-β3 increased cell spreading and appeared to reduce cell-surface contacts indicated by both SPR imaging and a detachment assay. TGF-β3 also caused a decreased cell alignment response to microcontact printed protein patterns, in part due to the deposition of ...

Stable recombinant cell line expressing the Muscarinic, M1 receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP). In addition, for total reassurance, we will provide up to two free replacements within in a 2 year period should you accidently lose your cell line ...

BALB/MK keratinocyte cell line cultured in a defined medium. b Overexpressed. c FRSK cell line. d PAM212 cell line. c HPK1A cell line.. duced. Approximately 106-107 cells are injected in 0.1 ml PBS into the interscapular region of the host.19 Most malignant keratinocytes can grow subcutaneously, whereas papilloma or benign tumor cells do not form tumors94 (Table III).. 91 D. A. Greenhalgh and S. H. Yuspa, Mol Carcinog. 1, 134 (1988).. 92 C. M. Kim, J. Vogel, G. Jay, and J. S. Rhim, Oncogene 7, 1525 (1992).. 93 E. Finzi, A. Kilkenny, J. E. Strickland, M. Balaschak, T. Bringman, R. Derynck, S. Aaron-son, and S. H. Yuspa, Mot. Carcinog. 1, 7 (1988).. 94 J. E. Strickland, D. A. Greenhalgh, A. Koceva-Chyla, H. Hennings, C. Restrepo, M. Balaschak, and S. H. Yuspa, Cancer Res. 48, 165 (1988).. Was this article helpful?. ...

Table of Contents. Cell Lines Derived from Human Tumors for Vaccine Manufacture. 1. Introduction……………………………………………………………………………..……………………………4. 2. Background………………………………………………………….………………………………………………..4. 2.1 History of Cell Substrates for Viral Vaccine Manufacture in the U.S.: Primary,. Diploid, and Tumorigenic Cells……………………………….…………………………………………………5. 2.2 Cell Substrates Derived from Human Tumors Proposed for Manufacture of. Viral vaccines……………………………………………………………………………………………………………6. 3. Considerations Regarding the Use of Tumor-derived Cell Lines……..…………………………8. 3.1 Mechanism of Tumorigenesis and Considerations for Characterization of ...

Fingerprint Dive into the research topics of Techniques for purifying L-cell karyoplasts with minimal amounts of cytoplasm. Together they form a unique fingerprint. ...

A. Left: A consolidated overview of PLSR‐derived variable importance in the projection (VIP) scores mapped onto a simple schematic of immediate‐early signaling, indicating the fraction of 10 studied cell lines in which early (1-10 h) or late (24-48 h) up‐ or down‐regulation of each of the cell state markers and phospho‐proteins is predictive of response to RAF/MEK inhibitors (72‐h viability). Up‐regulation or down‐regulation of signals with VIP score ,1 is shown. Right: Correlation between up‐ and down‐regulation of selected pathways in response to RAF/MEK inhibition across the 10 studied cell lines. Correlations between signaling changes in response to RAF/MEK inhibition were evaluated based on pairwise Spearmans correlation between VIP scores for selected phospho‐proteins at 24 and 48 h post‐treatment across the 10 studied cell lines. P‐values ,0.05 were considered significant. ...

Historically, the analysis of DNA replication in mammalian tissue culture cells has been limited to static time points, and the use of nucleoside analogues to pulse-label replicating DNA. Here we characterize for the first time a novel Chromobody cell line that specifically labels endogenous PCNA. By combining this with high-resolution confocal time-lapse microscopy, and with a simplified analysis workflow, we were able to produce highly detailed, reproducible, quantitative 4D data on endogenous DNA replication. The increased resolution allowed accurate classification and segregation of S phase into early-, mid-, and late-stages based on the unique subcellular localization of endogenous PCNA. Surprisingly, this localization was slightly but significantly different from previous studies, which utilized over-expressed GFP tagged forms of PCNA. Finally, low dose exposure to Hydroxyurea caused the loss of mid- and late-S phase localization patterns of endogenous PCNA, despite cells eventually completing S

Stable recombinant cell line expressing the Opioid, Kappa receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP ...

The FLYRD18 Packaging cell line enables production of high-titer, human complement-resistant recombinant retroviruses, with significantly reduced probability of replication-competent retrovirus

We describe a high, synergistic induction of IDO1 expression by the demethylating drug zebularine and IFN-γ in the human monocytic cell line THP-1. ► The ...

Webcat Plus: Development of membrane excitability during the in vitro differentiation of a clonal mouse myogenic cell line, 博士論文;博士論文

Dr. Barron: So, as I mentioned previously, there are two main approaches. Ideally, an industry would use this technology before going through regulatory submission. So, with a new cell line making a particular product of interest, a cell line would be manipulated stably by using something like the technology I just described. For instance, over expression sponge that would target the microRNA of interest, or indeed, over expressing a short hairpin that will mimic the microRNA of interest if over expression is desired. So, that forms a stable cell line that can act as the parent, into which the product gene or the transgene is placed and that would go through the normal regulatory process ...

AequoScreen® Double Transfected Cell Line: Human recombinant P2Y11 receptor in aequorin 1321N1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...

AequoScreen Double Transfected Cell Line: Human recombinant 5-HT3A receptor in aequorin HEK-293 host cell.. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay.. All cell lines are tested for the absence of mycoplasma.. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.. Features:. ...

This webinar will discuss the evolving role of Cell Lines and Cell Culture and their usefulness as models of physiological function and disease. The webinar will review the depth, breadth and best use of currently available Cell Lines. The role played by Cell Lines in ensuring that the output of future research continues to be valid and credible will also be evaluated. This is the first of a three part webinar series focused on delivering a broad picture of the role of cell lines as models in scientific research, the various strategies and approaches available.

Stable recombinant cell line expressing the Opioid, Mu receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP ...

CAMBRIDGE, MA and SEATTLE, WA, October 3, 2017－BlueRock Therapeutics and Universal Cells Inc. today announced that they have entered into a collaboration and license agreement to create induced pluripotent stem (iPS) cell lines useful for the manufacture of allogeneic cellular therapies.. BlueRock is at the cutting edge of the cell therapy field and our collaboration with the company represents an important step in their efforts to develop off-the-shelf cell therapies for degenerative diseases, said Claudia Mitchell, Ph.D., CEO of Universal Cells. We are honored that our technology will support the advancement of BlueRocks cell therapies.. Under the terms of the agreement, BlueRock and Universal Cells will collaborate to engineer iPS cell lines for clinical use. The agreement grants BlueRock commercial rights to use the engineered cell lines for undisclosed therapeutic applications.. This partnership with Universal Cells provides us with an important technology useful to advance ...

Applied StemCell offers a comprehensive custom service for lentiviral-based Stable Cell Line Generation for genetically engineering sensitive cells lines.

Human muscle cell line - human body diagram at muscles. Human Muscle Cell Line encouraged for you to our website, on this period I am going to teach you with regards to Human muscle cell line.. Now, this can be a very first image, human muscle cell line, human muscle cell line atcc, human smooth muscle cell line, human skeletal muscle cell line atcc, human cardiac muscle cell line, human heart muscle cell line, human vascular smooth muscle cell line, human airway smooth muscle cell line, human skeletal muscle myoblast cell line, immortalized human skeletal muscle cell line :. ...

Reduction of the cholesterol level in membranes of epithelial Madin-Darby canine kidney (MDCK) cells reverses the apical-to-basolateral transport ratio of the apical membrane marker protein influenza virus haemagglutinin and the secreted glycoprotein gp80. At the same time, basolateral transport of the vesicular stomatitis virus G protein is unaffected [Keller and Simons (1998) J. Cell Biol. 140, 1357-1367]. To investigate whether cholesterol depletion influences apical sorting mechanisms specifically, or apical transport capacity more generally, we studied the effect of cholesterol depletion on the secretion of three different classes of molecules from the apical and basolateral surfaces of MDCK cell layers: glycoprotein gp80, sulphated proteoglycans and proteins, and non-glycosylated rat growth hormone. In each case, cholesterol depletion reduced the fraction secreted to the apical medium and increased the fraction secreted basolaterally. The fact that this was observed for all sulphated ...

TY - JOUR. T1 - Autophagy is the predominant process induced by arsenite in human lymphoblastoid cell lines. AU - Bolt, Alicia M.. AU - Byrd, Randi M.. AU - Klimecki, Walter T.. PY - 2010/5/1. Y1 - 2010/5/1. N2 - Arsenic is a widespread environmental toxicant with a diverse array of molecular targets and associated diseases, making the identification of the critical mechanisms and pathways of arsenic-induced cytotoxicity a challenge. In a variety of experimental models, over a range of arsenic exposure levels, apoptosis is a commonly identified arsenic-induced cytotoxic pathway. Human lymphoblastoid cell lines (LCL) have been used as a model system in arsenic toxicology for many years, but the exact mechanism of arsenic-induced cytotoxicity in LCL is still unknown. We investigated the cytotoxicity of sodium arsenite in LCL 18564 using a set of complementary markers for cell death pathways. Markers indicative of apoptosis (phosphatidylserine externalization, PARP cleavage, and sensitivity to ...

G Berman, S L C Woo, D Burnett, R A Stockley; Alpha, Antichymotrypsin Synthesis by Alveolar Macrophages And Monocyte-Like Cell Lines. Clin Sci (Lond) 1 January 1987; 73 (s17): 2P. doi: https://doi.org/10.1042/cs073002P. Download citation file:. ...

TY - JOUR. T1 - Size and cell type dependent uptake of silica nanoparticles. AU - Hsiao, I-Lun. AU - Gramatke, A. M.. AU - Joksimovic, R.. AU - Sokolowski, M.. AU - Gradzielski, M.. AU - Haase, A.. N1 - BfROA. PY - 2014. Y1 - 2014. N2 - As silica nanoparticles (SiO2 NP) gain increasing interest for medical applications it is important to understand their potential adverse effects for humans. Here we prepared well-defined core-shell fluorescently labelled SiO2 NP of 15, 60 and 200 nm diameter and analyzed their cytotoxicity in THP-1 derived macrophages, A549 epithelial cells, HaCaT keratinocytes and NRK-52E kidney cells. We observed a size-dependent cytotoxicity in all cell types in serumfree conditions. HaCaT cells were least and macrophages or lung derived A549 cells were highly sensitive towards SiO2 NP treatment. Differences in cytotoxicity could be correlated with different uptake rates. By using flow cytometry and confocal microscopy we quantified the uptake. Furthermore we used specific ...

Cell culture. The human keratinocyte cell lines HaCaT and HaCaT-RG were generously provided by P. Boukamp (German Cancer Research Center, Heidelberg, Germany); the squamous carcinoma cell lines SCC-1, SCC-6, SCC-17B, and SCC-74B were gifts from T. Carey (University of Michigan, Ann Arbor, MI); the squamous carcinoma cell lines SCC-012 and SCC-028 were kindly provided by D. Sidransky (Johns Hopkins University, Baltimore, MD). Human epidermal keratinocytes (HEK) and normal human dermal fibroblasts (NHDF) were obtained from the Vanderbilt Skin Disease Research core. HaCaT, HaCaT-RG, SCC-1, SCC-6, SCC-17B, SCC-74B, HeLa [American Type Culture Collection (ATCC), Manassas, VA], A-431 (ATCC), and NHDF were cultured in DMEM supplemented with 10% FCS and 1% penicillin-streptomycin. SCC-012 and SCC-028 cells were cultured in RPMI 1640 supplemented with 10% FCS and 1% penicillin-streptomycin. The A-549 human lung adenocarcinoma cell line (ATCC) was cultured in DMEM supplemented with 10% FCS, 10 μg/mL ...

The role of the cytoplasmic domain of the human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins in virus replication was investigated. Deletion of residues 840 to 856 at the carboxyl terminus of gp41 reduced the efficiency of virus entry during an early step in the virus life cycle between CD4 binding and formation of the DNA provirus without affecting envelope glycoprotein synthesis, processing, or syncytium-forming ability. Deletion of residues amino terminal to residue 846 was associated with decreased stability of envelope glycoproteins made in COS-1 cells, but this phenotype was cell type dependent. The cytoplasmic domain of gp41 was not required for the incorporation of the HIV-1 envelope glycoproteins into virions. These results suggest that the carboxyl terminus of the gp41 cytoplasmic domain plays a role in HIV-1 entry other than receptor binding or membrane fusion. The cytoplasmic domain of gp41 also affects the stability of the envelope glycoprotein in some cell types. ...

Attaching and effacing (AE) adhesion is associated with the pathogenesis of enteropathogenic Escherichia coli (EPEC) and rabbit diarrheogenic E. coli (RDEC-1). Although RDEC-1 provides an animal model for investigating pathophysiology of EPEC infection, RDEC-1 does not adhere to human cell lines, thereby limiting in vitro investigation. Therefore, transformed RDEC-1 strains expressing adhesins derived from human diarrheogenic E. coli were studied. These adhesins promoted AE adhesion of RDEC-1 and led to the accumulation of alpha-actinin aggregates in the cytoplasm of infected cells. Furthermore, these strains induced host signal transduction pathways, resulting in tyrosine phosphorylation of host proteins and an intracellular elevation of calcium. These results demonstrate that RDEC-1 and EPEC stimulate similar signal transduction pathways in infected epithelial cells, thus lending additional support for the use of RDEC-1 as a model for the study of human EPEC infection.

Results of the present study show for the first time that Chk1 undergoes activating phosphorylation in marrow blasts in vivo during cytarabine-containing induction therapy. Building on this result, we also show in human AML cell lines that the selective Chk1 inhibitor SCH 900776 abrogates cytarabine-induced S-phase arrest, increases cytarabine-induced apoptosis, and enhances the effects of cytarabine on colony formation. Likewise, SCH 900776 increases the effects of cytarabine in a majority of primary AML isolates but not normal myeloid progenitors in vitro. This sensitization was observed at SCH 900776 concentrations far below the approximate 5 μmol/L SCH 900776 peak levels observed at the maximum tolerated dose in solid tumor patients. These observations have potentially important implications for current efforts to enhance the efficacy of cytarabine-containing AML regimens.. Previous results have shown that cytarabine activates the ATR/Chk1 checkpoint in tissue culture cell lines in vitro ...

The U937 cell line is an oncogenic human monocyte cell line. These monocytes have the potential of differentiating into either macrophages or dendritic cells (Lawrence et al., 2011). This differentiation pattern depends on the characteristics of the tissue microenvironment (Kigerl et al., 2009). PMA (Phorbol 12-Myristate 13-Acetate) is a phorbol ester capable of transforming monocytic cells toward the macrophage pathway. Upon treatment with PMA, U937 cells under-go a series of morphological and functional changes. Traditionally monocytic cell lines are used as a model of macrophage function, because current human macrophage cell lines require a T-cell conditioned growth medium and contact with irradiated peripheral blood leukocytes (PBLs) to propagate (Lee et al., 1997). The PMA-treated monocyte is referred to as macrophage-like, meaning that the properties of the transformed cell line are not yet fully understood (Dockrell et al., 2010). These macrophages are clinically significant for possible

TLR2/NF-kB reporter cell line/TLR2 cell line can be used to monitor the TLR2 signaling pathway activity. Screen for activators or inhibitors of the TLR signaling pathway.

The signal transduction pathway that leads to the activation of NF-kB is extensively studied because of its importance in immune system activation. Any molecule that interferes with the pathway is therapeutically important because of its potential role in triggering or inhibiting the immune response. To identify proteins that interfere with the activation of NF-kB, we designed and performed a genetic screening system of plant proteins. Our screen consisted of ankyrin repeat plant proteins expressed in mammalian tissue culture cells along with an NF-kB reporter vector that expressed Green Fluorescent Protein (GFP) upon NF-kB activation. As a tissue culture system we used the easily transfected human embryonic kidney cell line FEK293T. To perform our screen, we generated an NF-kB sensitive GFP reporter plasmid by standard molecular biology cloning techniques and we constructed a library of mammalian expression vectors that contained 90 A. thaliana cDNAs by Cre-mediated recombination between ...

Murine fibroblasts expressing viral receptors and human cyclin T1 allow HIV-1 entry and viral gene expression but do not support efficient assembly. A chimeric HIV-1 carrying a non-homologous matrix (MA) from murine leukemia virus in place of HIV-1 MA can assemble efficiently in murine cells, yet has poor infectivity. Here, we assess the ability of a homologous MA from SIV MAC239 to complement assembly and infection in chimeric viruses designated SHIV(MA). The resulting SHIV(MA) chimeras produce more virus than native HIV-1 when transfected into murine cells. SHIV(MA) exhibits cell-type-specific replication in human T cell lines, replicating well in MT4 cells and poorly in Jurkat cells due to an incompatibility with the HIV-1 Env. The infectivity defects of SHIV(MA) are rescued by pseudotyping with VSV-G but not by truncation of the cytoplasmic tail of Env. Passage of SHIV(MA) in Jurkat cells produces variants with improved Env incorporation and improved replication in Jurkat but not in 3T3 TXC ...

Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNγ+LPS) or Alternatively Activated M(IL-4) Macrophages Abstract.

On every occasion, a cell that is maintained in a laboratory faces the risk of contamination with another cell, particularly a rapidly growing and continuous cell line. The first case of cross-contamination in cell culture was reported in 1950s and since then cross contamination (and accidental co-culture) remains a disturbing issue in cell culture laboratories, as contamination of a cell line with another cell line is not readily detectable like the bacterial and fungal contamination.. Among the various cell contaminants, HeLa, the oldest and most commonly used human cell line that was derived from the cervical cancer cells from Henrietta Lacks, taken by George Gey, is perhaps the commonest contaminating cell in cell culture laboratories. The International Cell Line Authentication Committee (ICLAC) database of 475 Cross-Contaminated or Misidentified Cell Lines - originally developed by Amanda Capes-Davis and Ian Freshney (published in 2010) and curated by ICLAC - lists 138 different cell ...

Cancer is a genetic and epigenetic disease that involves inactivation of tumor suppressor genes and activation of proto-oncogenes. All-trans retinoic acid (ATRA) is an isomer of retinoic acid involved in the onset of differentiation and apoptosis of a number of normal and cancer cells, functioning as an anti-cancer agent in several neoplasms. Ectopic changes in the expression of certain microRNAs (miRNAs) occur in response to ATRA, leading to phenotypic alterations in neoplastic cell lines. Moreover, the modulation of miRNA patterns upon ATRA-treatment may represent an effective chemopreventive and anti-cancer therapy strategy. The present systematic review was performed to provide an overview of the modulation of ATRA-induced miRNA expression in different types of neoplastic cells and identify the efficacy of intervention factors (i.e., concentration and duration of treatment) and how they influence expression profiles of oncogenesis-targeting miRNAs. A systematic search was conducted according to the

Genome-wide genetic information in about 1,000 cancer cell lines is available on COSMIC DB（Sanger Center, UK）, and on recent NGS analyses（Klijin C et al., Nat Biotechnol 2015）; however, among them, only 28 cell lines are derived from GC. Since driver gene mutation frequency in a certain cancer is often less than 5%, the establishment of cell lines from each patient to be analyzed is desired for functional selection of driver gene mutations. Furthermore, almost all of the 28 GC cell lines were established many years ago, thereby, the clinical and pathological information is insufficient. The wait is on for the establishment of new GC cell lines, especially from metastatic sites after therapy. Peritoneal metastasis is most frequent in GCs, especially diffuse-type GCs. In 2016, we successfully established 13 diffuse-type GC cell lines from the cancer ascites of 12 patients. In collaboration with the Division of Genetics, we have totally established 84 diffuse-type GC cell lines（National ...

Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...

Keratinocytes and neurons cells are the main target for Herpes Simplex Virus Type 1 (HSV-1) invasion. Moreover, keratinocytes are the most abundant cell types in the epidermis layer in the skin. Therefore, they are the first cells to encounter HSV-1 in the primary infection. Next, the virus reaches the nerve endings and is transferred to neuronal cells as a result from the primary infection. In between these two events, innate immune cells including monocytes and macrophages response is activated and recruited to the infection site. In this study, keratinocytes (PAM-212) and murine macrophage (RAW 264.7) cell lines were utilized to investigate the response of macrophages (RAW 264.7 ) and keratinocytes (PAM-212) to HSV-1 infection and propagation in vitro. In this study, initially keratinocytes (PAM-212) and macrophages (RAW 264.7) and were studied either infected or uninfected with HSV-1 at MOI 0.1 in a monolayer model. Cell lines were co-cultured at ratio 1:5 (macrophages : keratinocytes) respectively

EBV transformed B cell lines derived from Japanese (HEV). ● We are providing B cell lines derived from healthy Japanese and the Japanese patients suffering from cancers etc. The cell lines derived from healthy individuals possess the precise information regarding HLA haplotypes and we are providing such information as well.. ● These cell lines are useful for genetic analyses, and thus we are providing these cell lines for researches including analyses of gene and genome. We provide this collection not only to domestic users in Japan but also to users in overseas, in addition not only to not-for-profit institutions but also to for-profit institutions.. ● Providing B cell lines were transformed by Epstein-Barr Virus.. ● In addition to the cell material itself, we are providing information about gender, age and habit of the individuals who have donated the source cells. When we possess the information of gene and/or genome, we provide them as well.. ● After the providing procedures ...

The HBL and LND1 cell lines, wt for BRAF, highly express PGC1alpha while hmel1, hmel9, hmel11, Mba72, M3, presenting BRAF mutations at the V600 residue, show a downregulation of this gene. MITF expression levels were more abundant in HBL and LND1 cell lines with respect to the other cell lines harbouring BRAF mutations. There is a direct correspondence between PGC1alpha and MITF levels: higher levels of PGC1alpha are associated with an enhanced MITF quantity. The analysis of cAMP levels in our melanoma cell lines showed a similar trend, being higher in wt BRAF cell lines compared to the other cell lines. ...

SHIRLEY - AUG 30, 2017 - Creative Bioarray, an innovative biotechnology company which concentrates on developing unique technologies for products and service, including professional cell line authentication that will help verify the identity of human cell lines, ensuring uniqueness of the cell line and detecting laboratory errors such as misidentification and cross-contamination of lines.. The first human cancer cell line was established in 1952. However, researchers found that many of the cultured cells had different characteristics from the original cell lines in later experiments, and that some methods of cell culture could lead to cell changes or cross-contamination. Cell culture system is very important in the research and development of bio-pharmaceuticals, the appropriate cell line authentication process gets a lot of attention from researchers. However, cell cross-contamination still is inevitable which affects every area of scientific research - from the bench to clinical trials. Cell ...

A series of 22 neuroepithelioma and neuroblastoma cell lines were screened for expression of nerve growth factor receptor (NGFR) by flow cytometry, Western blotting, and Northern blotting. All 5 neuroepithelioma cell lines expressed cell surface NGFR, with 30-69% of cells NGFR positive, but the 17 neuroblastoma cell lines tested had a smaller percentage of cell surface NGFR-positive cells (0-21%) and 10 lines were completely lacking cell surface NGFR. SY5Y, a variant line with a neuronal phenotype derived from neuroblastoma line SKNSH, expressed much more NGFR than SHEP, a variant line with an epithelial-like phenotype also derived from SKNSH. By Western blotting, the Mr≈69,000 NGFR band was detected for all four neuroepithelioma cell lines tested but was visible for only 8 of 15 neuroblastoma cell lines tested. The band was most intense for neuroepithelioma cell lines SKNMC and TC32. For these two lines, a Mr≈56,000 and a Mr≈60,000 band were also detected. By Northern blotting, all three ...

Cell Culture. The murine macrophage cell line J774A.1 was grown in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamicin, 20 U/ml polymyxin B, and 10% fetal bovine serum. Alternatively, peritoneal macrophages were isolated 5 days after injection of 2 ml of Brewers thioglycolate medium (Sigma-Aldrich, St. Louis, MO) into the peritoneal cavity of C56BL/6 mice as reported previously (McCarron et al., 1984). Primary macrophages were added to culture flasks and allowed to adhere for 2 h at 37°C. Nonadherent cells were removed by three washes of warm medium. Adherent cells were ,99% macrophages as assessed by immunocytochemical detection of the macrophage marker F4/80 (anti-F4/80, clone Cl:A3-1; Serotec, Oxford, UK). Smooth muscle cells were isolated from mouse or rabbit aorta by collagenase type 2 (Worthington, Lakewood, NJ) and elastase (Sigma-Aldrich) digestion (60-90 min at 37°C) at 300 and 5 U/ml final concentration, ...

Two lineages of Zika virus (ZIKV) have been classified according to the phylogenetic analysis: African and Asian lineages. It is unclear whether differences exist between the two strains in host cell permissiveness, this information is important for understanding viral pathogenesis and designing anti-viral strategies. In the present study, we comparatively studied the permissive spectrum of human cells for both the African (MR766) and Asian strains (PRVABC59) using an RNA in situ hybridization (RISH) to visualize RNA replication, an immunofluorescence technology, and a western blot assay to determine viral protein production, and a real-time RT-PCR to examine viral RNA multiplication level. The experiments were undertaken in the condition of cell culture. We identified several human cell lines, including fibroblast, epithelial cells, brain cells, stem cells, and blood cells that are susceptible for the infection of both Asian and African strains. We did not find any differences between the MR766 and the

Primary mouse epidermal cells underwent spontaneous ma lignant transformation in culture. Twelve malignant epidermal cell lines were established which produced squamous cell carcinomas in syngeneic hosts. These lines were used to define criteria for recognizing transformed epidermal cells in vitro. Growth in suspension in agar, agarose, or Methocel was mini mal for 11 of the lines. All lines tested retained specific epider mal antigens (pemphigus, pemphigoid, keratin) by indirect immunofluorescence, but keratin content was reduced when quantified by radioimmunoassay. Basal activity of ornithine decarboxylase and activity induced by the tumor promoter 12O-tetradecanoylphorbol-13-acetate were variable among lines. All malignant lines as well as normal epidermal cells grew well at reduced extracellular calcium concentrations. When the extracellular calcium was elevated, normal cells ceased prolif eration, terminally differentiated, and sloughed from the culture dish, while malignant cells continued to

ESI BIOs hES cell lines are among the best-characterized and documented lines available today. These research grade cell lines have also been approved by the National Institutes of Health (NIH) and are included in the NIH Stem Cell Registry.

Thomas, N, Dupont, E, Halliday, D, Fry, CH and Severs, NJ (2006) An inducible cell system to investigate connexin co-expression and action potential propagation within the heart In: 28th Annual International-Society-for-Heart-Research North American Section Meeting, 2006-06-13 - 2006-06-16, Toronto, CANADA. Full text not available from this repository ...

Interferon-induced transmembrane proteins (IFITMs) are a family of host restriction factors that affect the fusion capacity of many enveloped RNA viruses. A preprint by Prelli Bozzo et al. now reports that SARS-CoV-2 appears to hijack IFITMs, and in particular IFITM2, as cofactors for the infection of lung cells. Whereas overexpression of IFITMs impaired SARS-CoV-2 membrane fusion with HEK293T cells in vitro, knockdown studies in the human lung epithelial cell line Calu-3 showed that endogenous levels of IFITM1, IFITM2 and IFITM3, both in the absence or presence of interferon, boost SARS-CoV-2 infection, with IFITM2 showing the strongest effect. ...

Im a little bit confused about the LTK- cells. Does this mean that normal fibroblast cells would use a tyrosine kinase pathway for cell growth, but they lack the enzyme to do so, therefore GPCR is the pathway they used? The cells are a mouse fibroblast cell line which can be used safely on the bench. The cells express beta adrenergic receptors. You should focus on the effects of the drugs themselves and the fact that LTK- cells express these receptors ...

Chemotherapy is one of the major treatment modalities for cancer. Metal-based compounds such as derivatives of cisplatin are in the front line of therapy against a subset of cancers, but their use is restricted by severe side-effects and the induction of resistance in treated tumors. Subsequent research focused on development of cytotoxic metal-complexes without cross-resistance to cisplatin and reduced side-effects. This led to the discovery of first-generation titanium(IV)salan complexes, which reached clinical trials but lacked efficacy. New-generation titanium (IV)salan-complexes show promising anti-tumor activity in mice, but their molecular mechanism of cytotoxicity is completely unknown. 4 different human cell lines were analyzed in their responses to a toxic (Tc52) and a structurally highly related but non-toxic (Tc53) titanium(IV)salan complex. Viability assays were used to reveal a suitable treatment range, flow-cytometry analysis was performed to monitor the impact of dosage and treatment

This is part of a triplicate data set of non-overlapping fields of NIH 3T3 fibroblasts cultured on polystyrene. Each set is indicated by the well num...

This is part of a triplicate data set of non-overlapping fields of NIH 3T3 fibroblasts cultured on polystyrene. Each set is indicated by the well num...

The washing process with PBS was repeated and the final centrifugation was performed (1200 r. Kratom Get High Kratom Get High Clifton Park Clifton Park c until further analysis. The cell lysates and protein determination were carried out prior to immunoblot kratom army drug test analysis.. The basic toxicology data established in the previous chapter has informed us on the potential cytotoxicity of MSE and MIT on several human cell lines which generally shows cytotoxicity with high dose. The lethal effect of the extract and major alkaloid (MIT) on the cells examined prompted the question whether cell death was accompanied by DNA damage. DNA damage as a result of endogenous sources (cellular metabolic processes) or exogenous sources (environmental factors such as chemical insult) could lead to reversible or kratom vs phenibut irreversible genetic change. Based on the long term use of this plant by humans testing for its genotoxic potential using mammalian cells was thought to be more appropriate ...

Summary Four clones of the hamster cell line NIL-2 were transformed by adenovirus 12. The transformed cells formed foci of multilayered growth in monolayer cultures under agar medium and colonies when suspended in soft agar medium. A virus stock containing 5 × 109 particles and 1.1 × 109 p.f.u./ml. contained 2.8 × 102 focus-forming units (f.f.u.)/ml. in NIL-2 cells. The numbers of cell foci and of colonies induced by serial dilutions of virus were consistent with a linear dose response. Approximately 2 × 107 total virus particles or 4 × 106 infectious units were required to induce one focus of transformed cells. The highest transformation rate obtained was 0.002% for cells exposed to about 80 p.f.u. of virus per cell. For comparison, primary rat embryo cells were transformed by adenovirus 12. The results obtained were approximately the same as those with NIL-2 cells except that the rat cells did not form colonies when infected and suspended immediately in agar. However, rat cells transformed in

Tyrosine-specific phosphorylation of the receptor for epidermal growth factor (EGF) in plasma membranes isolated from WI-38 cells is EGF-dependent and occurs to an equivalent extent and on identical tryptic peptides in preparations from cells of various in vitro ages. There is a marked reduction, however, in phosphorylation of receptor molecules from senescent as compared with young WI-38 cells, if enzyme activity is assayed in an immune complex following solubilization of plasma membranes with Nonidet P-40 (NP-40). Differences in the level of receptor phosphorylation in young vs. senescent NP-40 extracts are not resolved by changing the temperature at which the assay is performed, or the length of incubation. Moreover, addition of NP-40 or chloroform-methanol extracts of young cells to assays measuring receptor phosphorylation in senescent cell NP-40 preparations does not augment the senescent enzyme activity. The immunopurified senescent receptor is, however, capable of catalyzing

In 1994, OSHA issued an interpretation clarifying the applicability of the Bloodborne Pathogen Standard to human cell lines. According to the interpretation, human cell lines are considered to be potentially infectious and within the scope of the BBP Standard unless the specific cell line has been characterized to be free of hepatitis viruses, HIV, Epstein-Barr virus, papilloma viruses and other recognized bloodborne pathogens ...

This study showed the successful CRISPR/Cas9 bioengineering of a human liver cell line, HuH-7, to create new cell lines that express the common CYP3A5 *1 variant that is known to be highly relevant toward drug metabolism. Unlike recent reports using CRISPR/Cas9 to knock out CYP2E1 (Wang et al., 2016) or CYP3A1/2 (Lu et al., 2017) function in rats, we used CRISPR/Cas9 to activate CYP3A5 expression in human cells lines by conversion of *3 to *1 genotype. This is the first report of engineered cell lines for both heterozygous and homozygous CYP3A5 *1 expression in human liver cell culture and phenotypic analysis.. This study showed that it is possible to use two methods of CRISPR/Cas9 biotechnology to modify the HuH-7 cells to express CYP3A5 *1 by splice junction deletion using two gRNAs or with one gRNA and a homology-directed repair template. Without the need for fluorescent- activated cell sorting or less precise limiting dilution techniques, a soft agar clonal selection with expansion on ...

sciencehabit writes: In a new study, researchers led by Edward Chuong, a computational biologist at the University of Utah in Salt Lake City, explored whether endogenous retroviruses (ERVs) help us fend off invaders. The scientists scanned three different human cell lines for ERVs in their DNA that...

Stable recombinant cell line expressing the Neuropeptide S NPS receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP ...

What came first the chicken or the egg and does lifes trials and tribulations make the lines or your palms or do the lines predestine your fate? Now in Japan you can change your future by lengthening the life line on your palm or adding money-luck or marriage lines to your palm. Men usually wish to change their business related success lines, such as the fate line（運命線), the money-luck line (金運線) to make profits, and the financial line (財務線) to save what money you make. These three lines, when they come together just right, create what is called the emperors line. Women usually request marriage or romance lines on their palms ...

The AhR HepG2 and AhR HEK293 stable cell lines were assayed for RenSP luciferase following treatment with omeprazole. The HEK293 cell line shows a weaker response to omeprazole than the HepG2 cell line because HEK293 cells express a lower level of the aryl hydrocarbon receptor (AhR).. ...

Serial chromosome studies were performed on four monocytic cell lines established from bone marrow samples of patients suffering from hematopoietic disorde