By Adam Bonislawski A team led by Swiss Federal Institute of Technology Zurich researcher Ruedi Aebersold has published a quantitative characterization of the proteome of the human tissue culture cell line U2OS.
A glass-adherent human lymphoma cell line was found to produce an inhibitor of human in vitro lymphocyte blastogenic responses. The responses to mitogens, antigens, and allogeneic leukocytes were inhibited over 90%, as assayed by DNA synthesis or morphology. The effect was not associated with cytotoxicity and was reversible by washing the inhibited cells. The material was a nondialyzable, heat-stable protein. Its activity was not affected by its deoxyribonuclease and ribonuclease but was destroyed by Pronase. Most important, the inhibitor was species and tissue specific; it did not inhibit mouse lymphocytes or a variety of human tissue culture cell lines. The relationship of this material to regulation of lymphoid function and to the etiology and pathogenesis of cancer is discussed.. ...
In the present work we studied the expression of membrane-bound Ig (MBIg) as well as receptors for IgG Fc and complement on nine human lymphoblastoid cell lines. When MBIg and receptors for IgG Fc were compared, four categories of cell lines could be distinguished: (a) cell lines having both MBIg and receptors for IgG Fc, (b) cell lines having MBIg but lacking receptors for IgG Fc, (c) cell lines lacking MBIg but having receptors for IgG Fc, and (d) cell lines lacking both MBIg and receptors for IgG Fc. Two types of receptors for complement could be detected on the cell lines studied, one for C3-C3b and one for C3d. When sensitized red cells carrying C3b or C3d were used for rosette tests, three categories of cell lines could be distinguished: (a) cell lines having receptors for C3b and C3d, (b) cell lines having receptors only for C3d and (c) cell lines lacking both receptors. However, when a more sensitive immunofluorescent method was used instead of the rosette technique, it was found that ...
TY - JOUR. T1 - Signal transduction pathways of GM-CSF in neural cell lines. AU - Choi, Jung Kyoung. AU - Choi, Byung Hyune. AU - Ha, Yoon. AU - Park, Hyeonseon. AU - Yoon, Seung Hwan. AU - Park, Hyung Chun. AU - Park, So Ra. N1 - Funding Information: This study was supported by a grant of the Korea Health 21 R&D Project, Ministry of Health & Welfare, Republic of Korea (A050082). PY - 2007/6/15. Y1 - 2007/6/15. N2 - GM-CSF is recently being suggested to play important role(s) in the nervous system. Present study was intended to understand signal transduction pathways of GM-CSF in human neuroblastoma (SK-N-(BE)2) and glioblastoma (A172) cell lines. The expression of GM-CSF receptors on the surface of these cells was confirmed by immunocytochemistry, Western blot analysis and RT-PCR. When treated for 10 min, GM-CSF activated the signal transducer and activator of transcription 5 (STAT5) and extracellular signal regulated kinase (ERK) in both cell lines. However, Janus kinase 2 (JAK2) was activated ...
Cross-contaminated cell lines could be causing us to waste millions or more on flawed research. And many scientists dont even know it.
Western blot analysis for several different cell lines indicating relative expression levels. These cells were derived from 3 different colonies that had alread
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Oncogenic activation of ras results in changes in the transcription of several genes leading to uncontrolled cell growth. In this paper, we demonstrate that transformation of fibroblast cells by the ras oncogene leads to transcriptional repression of the smooth muscle α-actin promoter. Transient transfection analysis of plasmids containing the 5′ upstream region of the human α-actin gene fused to human growth hormone or bacterial chloramphenicol acetyltransferase coding sequences into Rat-2 and ras-transformed Rat-2 (HO6) cells indicates that α-actin promoter is repressed in ras-transformed cells. In addition, stable rat fibroblast cell lines expressing human growth hormone or β-galactosidase under the control of α-actin promoter exhibit repressed reporter gene activity following transformation by the ras oncogene. α-Actin promoter-driven β-galactosidase activity is derepressed in revertants of ras-transformed stable cell lines. This revertant cell line expresses elevated levels of ras ...
May be imported without a USDA permit.. In addition, monoclonal antibodies intended for in vivo human use do not require a permit.. However, (1) cell lines derived from livestock or avian species, (2) cell lines derived from any species which will be used for in vivo use, and (3) cell lines of any species which may have been exposed to exotic livestock or avian disease agents will require a USDA, VS import permit.. Procedures:. A USDA, VS import permit will NOT be required for cell lines other products of cell lines, including monoclonal antibodies if the shipment is accompanied by:. ...
TY - JOUR. T1 - Cancer-derived p53 mutants suppress p53-target gene expression - Potential mechanism for gain of function of mutant p53. AU - Vikhanskaya, Faina. AU - Lee, Ming Kei. AU - Mazzoletti, Marco. AU - Broggini, Massimo. AU - Sabapathy, Kanaga. PY - 2007/3. Y1 - 2007/3. N2 - Tumour-derived p53 mutants are thought to have acquired gain-of-function properties that contribute to oncogenicity. We have tested the hypothesis that p53 mutants suppress p53-target gene expression, leading to enhanced cellular growth. Silencing of mutant p53 expression in several human cell lines was found to lead to the upregulation of wild-type p53-target genes such as p21, gadd45, PERP and PTEN. The expression of these genes was also suppressed in H1299-based isogenic cell lines expressing various hot-spot p53 mutants, and silencing of mutant p53, but not TAp73, abrogated the suppression. Consistently, these hot-spot p53 mutants were able to suppress a variety of p53-target gene promoters. Analysis using the ...
ECACC primary cell lines are offered from a variety of mammalian sources. Human cell lines, mouse cell lines, cancer cell lines and more are available. Sigma-Aldrich has partnered with The European Collection of Cell Cultures, ECACC, a world leader and recognized expert in the maintenance, cultivation and distribution of authenticated cell lines.
We were interested in the question of whether it might be possible to use knowledge of cancer-related mutations in the cell lines of the NCI60 screening data set to identify small molecules that preferentially inhibit the growth of cell lines containing either BRAF or KRAS oncogene mutations. Our hypothesis was that this cell line mutation knowledge could help to identify small molecules that were more likely to preferentially inhibit growth of cell lines with a particular mutation. It seems that any such molecules might be further investigated to try to better understand the molecular mechanisms of growth inhibition.We defined a quantity, \(\text{Diff}_{\text{mut}}\), that estimates how much more a given small molecule inhibits cell lines with a mutation of interest than cell lines without that mutation. We ranked the small molecules in descending order of \(\text{Diff}_{\text{mut}}\) and then tried to explain whether the ranking of the highest ranked molecules made sense in terms of independent facts
View Notes - 2010 Bio 317 Lecture 12 from BIO 317 at SUNY Stony Brook. Rush 10/15/10 11 1. How does a chemical messenger mediate a response in a particular cell type? 2. How does a chemical messenger
Shirley, NY-Profacgen, a world-leading custom protein service provider for a broad spectrum of industrial and academic clients with a commitment to delivering high-quality data and customer services, is pleased to update its stable cell line construction services. Scientists in the field of protein expression now have access to Profacgens latest stable cell line construction service version supported by cutting-edge facilities.. When large amount of recombinant protein is required, stable expression line construction is recommended for reproducible and cost-effective protein production. Generally, stable cell line construct will take 3 steps: delivery of the target gene into the host cell, screening of stable expression clones, and stability test. For efficient gene delivery and to achieve high integration rate, the company uses lentiviral vector. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When ...
Cambridge, UK and Liege, BE, 8 March 2017 - Horizon Discovery Group plc (LSE: HZD) (Horizon), the world leader in the application of gene editing technologies, including the provision of bioproduction cell lines, today announced it has entered into a research and development collaboration, and license agreement with AmplyCell S.A., to evaluate the impact of AmplyCells BOOST cell line optimisation technology on Horizons parental GS (glutamine synthetase) null CHO K1 cell line. AmplyCells Cell Fitness Technologies, which include BOOST, consist of a four-stage process to optimise cell lines in terms of stability and productivity. Cell lines can be stimulated using AmplyCells Cell Fitness Technologies at any stage of cell line development or bioproduction (clone creation, RCB/DCB, MCB), without the need to change culture conditions. Horizon is applying a disruptive business model alongside its expertise in genome editing to improve the manufacture of biotherapeutics, making its cutting-edge ...
Because buying a new cell line is a major investment for most researchers, this trial program is a great way to test a product while saving valuable research money. The BPS rental program includes more than 40 different cell lines. These cells can be rented for three months for 50% of the purchase price. After the leasing phase, the cell line can either be bought by paying the remaining 50% of the price, or the rental period ends and the cell line must be destroyed. With this option, the risk for the customer is reduced because the full price is only paid if the desired cell line has already been validated in their own laboratory.. ...
Read user reviews, compare products & request pricing from manufacturers of Yeast Cell Lines | Cell Lines, Stem Cells and Primary Cells
Standard Letter: Rejection of Cell Line. ,Sender,. To. ,Receiver,. ,Ref., DD.MM.YYYY. Dear Madame/Sir. On the ,date, we received the plant cell line ,name and designation, from your laboratory for deposit in the plant cell culture collection. Since no long term preservation method is presently available for the cell line it would have to be maintained in the living state at the collection centre.. Since we recently got the information that the maintenance of the cell line under conditions acceptable for CABRI is not possible we hereby inform you that we halted the accession procedure and discarded those samples of the cell line still maintained in our collection.. We apologize for being not able to include your cell line in our collection and remain. With kind regards. Yours Sincerely. ,Name of responsible ...
Fast stable cell line generation service for antibody production: development of suspension CHO stable cell lines for antibody production. No virus involved. DNA Transfection and stable cell line screening only.
[ I wrote this back in 2002] Host cell lines are an entirely new class of disease-causing organism. They are strange and unusually disgusting, and if you read this you may end up showering in Lysol for the next few weeks. Consider yourself warned. A host cell line is a microorganism that was until fairly…
Stable recombinant cell line expressing the Serotonin, 5HT2c (non-edited) receptor. Human recombinant, in 1321N1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP). In addition, for total reassurance, we will provide up to two free replacements within in a 2 year period should you accidently lose your cell line ...
organizing cell lines - posted in Cell Biology: Hi, Were a lab of 8 and were working with 10+ more types of cell lines, all from one cancer tyep though. Ive been put in charge of looking after these cell lines. I would welcome any experience in how to record the cell line stock in liquid nitrogen.
creat a SY5Y stable cell line - posted in Cell Biology: Hi everyone! I want to creat a stable cell line using SY5Y cells. I will do the transfection with the lentivirus next week and ill do selection with G418..someone has an idea how long in average does it take until i can freeze them for the first time? Thanks and good luck with your culturing Andreia
Our BacMam Histone H3 Cellular Assay Kits allow you to choose the modification that corresponds to your enzyme of interest in your chosen cell background or multiple cell backgrounds. Our BacMam Histone H3 Cellular Assay Kits allow you to choose the modification that corresponds to your enzyme of interest in your chosen cell background or multiple cell backgrounds.
A) Chemical structure of NVP-CGM097 and NVP-CFC218. (B) In vitro activity of NVP-CFC218 and NVP-CGM097 in TR-FRET binding assay (a) and cellular proliferation assay in human cancer cell lines (b). Data are expressed as concentration causing 50% inhibition and shown as mean ± SD from multiple (n≥8) independent experiments. (c) Selectivity is determined by the ratio of GI50 obtained using the HCT-116 p53-null and the HCT-116 p53WT isogenic pair of cell lines. (d) Selectivity is determined by the ratio of GI50 obtained using SAOS-2 (p53-null) and SJSA-1 (p53WT and HDM2-amplified) osteosarcoma pair of cell lines. (C and D) Scatter plot showing IC50 values expressed in μM of NVP-CFC218 in cell viability assays of p53 wild-type cell lines (C) and p53 mutated cell lines (D), colored by their response to NVP-CFC218. The data used to generate these plots, as well as cell line identity are available in Figure 1-source data 1. (E) Contingency table indicating the total number of sensitive and ...
The T47D/S2 Cell line is the control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 lines. Antiestrogen resistance is
Stable recombinant cell line expressing the Adrenergic, B3 receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP). In addition, for total reassurance, we will provide up to two free replacements within in a 2 year period should you accidently lose your cell line ...
Stable recombinant cell line expressing the Angiotensin, AT2 receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP). In addition, for total reassurance, we will provide up to two free replacements within in a 2 year period should you accidently lose your cell line ...
Long range regulatory interactions among distal enhancers and target genes are important for tissue-specific gene expression. Genome-scale identification of these interactions in a cell line-specific manner, especially using the fewest possible datasets, is a significant challenge. We develop a nove …
How can I find out if my cell line is free of pathogens? What pathogens should I be concerned about?. If you bought the cells from a vendor or a culture collection, then you can consult their catalog. Many catalogs will list safety and pathogen information -- if you cannot find it, contact the vendor. If you received the cells from another lab, you should find out where they originally came from.. Cell lines can contain harmful viruses. Sometimes, the viral genome is integrated into the cells genome. Most viruses have a limited host range, which means that they can only infect closely related species. Therefore, viruses living in a human or monkey cell line are likely to be dangerous to humans, but viruses living in an insect cell line probably cannot infect humans. If you work with a cell line from humans or other primates, you should check whether it contains viruses or viral genomic DNA. Viruses have Risk Group numbers, so if your cell line contains any viruses, you must handle it at the ...
Is there a secret to getting a cell background image to show up in Netscape 4.x? It looks fine in IE, but the cell color shows up instead of the image in Netscape. Heres my CSS code: TD.bkgrnd2 { background-color: #999999; background-image: url(../comp13_images/blue3.jpg); background-repeat: no-repeat; padding: 5px; }
Stable recombinant cell line expressing the Serotonin, 5HT-1A receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP ...
1) The main issue with this paper is this: these cell lines, although originally human, are all immortalized cancer cell lines characterised by markedly different biological properties when compared to normal human cells of the same tissue/cell type. They cant be readily used a surrogates for normal human tissue/cell types. None were primary cells nor were any even from recently acquired tissue samples from biopsies etc. People have infected primary human airway epithelial cultures with hCoV-EMC - so this can be done successfully - , although it would be more difficult for other tissue types as these cultures havent been developed. Some of these cell lines used may by chance lack key viral repressors of infection present in normal primary cells, which could skew results from cell culture infection experiments. Plus, a human tissue is not just a single cell type - they are composed of diverse kinds of cells that could together behave much, much differently than cell lines in culture ...
Horizons X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype.. ...
Horizons X-MAN® (Mutant And Normal) cell lines are genetically engineered cell pairs containing defined genomic modifications, providing a model system for the study of the role of genes in cellular processes and drug responses. Cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the genotype.. ...
Stable recombinant cell line expressing the Nicotinic, HM74a receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP ...
The HRA-19a1.1 Alpha2Alpha1 B cell line is a genetically modified clone of HRA-19a1.1 cells expressing a chimeric alpha2 integrin with
Posted by Dave Xanatos on 18th Nov 2019 I have been very pleased with my investment in this course and the support I have received so far. Josiah is great at taking his years of experience in this field and distilling it to some functional procedures that can help advanced beginner or intermediate students to gain working procedural knowledge of one of the most important areas of knowledge in biology - the very code of life itself. This will play an ever increasingly large role in the future of medicine, agriculture and what it means to be human. Josiah has worked closely with others and developed a course around somewhat unconventional methods that can allow people to do this work at home with a minimum of equipment that would ordinarily be prohibitively expensive or require specialized skills to build. This course is ideally suited for folks who have some basic working knowledge of molecular biology procedures and can be self-directed and disciplined in their approach to this work. This is not ...
Reducing drug development timelines and cost is a goal that we hear repeatedly in the industry. One area where timing is crucial is in reaching a go/no go decision on a therapeutic candidate and efficient production scale up once a go decision has been reached.. Early in development, small amounts of protein are needed to conduct preliminary studies that inform a go/no go decision. Protein can either be generated using transient production or through stable cell line generation. Most often companies choose to use transient transfection in either HEK or CHO cells to produce small quantities of protein because it is faster and more cost effective than developing a cell line. Having access to preclinical material faster and with less cost can greatly impact the overall drug development timeline. Then if the product progresses further in development, a stable cell line will be generated for larger scale manufacturing.. However, the timing of when to generate the stable cell line can be tricky. ...
Read user reviews, compare products and contact manufacturers of Cell Lines products, including eukaryote, prokayote and stem cell lines on SelectScience.
TY - JOUR. T1 - Monoclonal antibody to a nucleolar antigen of human B-lymphoblastoid cells. AU - Todorov, I. T.. AU - Philipova, R. N.. AU - Zhelev, N. Z.. AU - Hadjiolov, A. A.. PY - 1987/3. Y1 - 1987/3. N2 - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of rRNA transcription with Actinomycin D caused redistribution of the 41 5.6 antigen, but even at long term drug action it remains associated with the nucleolar remnants.. AB - An anti-nucleolar monoclonal antibody reacting with human B-lymphoblastoid cells but not with normal periferal blood lymphocytes has been isolated. The antibody recognized in Namalwa cells an antigen with molecular mass 41 kDa and pI 5.6, different from all previously described nucleolar antigens. Inhibition of ...
Human immortalized keratinocyte cell line HaCaT. Cultures of HaCaT cells were kindly provided by Dr. Norbert E. Fusenig (Institute of Biochemistry, German Cancer Research Center) and were grown on DMEM supplemented with 1% L-glutamine, 25 mM HEPES, 10% FBS and 1% penicillin-streptomycin at 37°C in a humidified incubator with 5% CO2 atmosphere (10, 14).. Assessment of cell morphological changes and viability. HaCaT cells at the density of 2×105 cells/well were placed onto 12-well plates and incubated at 37°C for 24 h before being treated with different concentrations of citric acid for 24 h; 0.5% of DMSO (vehicle) was used as a control. At the end of incubation, cells were examined and photographed under phase-contrast microscopy for determination of morphological changes. Cells (1×105 cells per sample) were then centrifuged at 1000 ×g for 5 min, and cell pellets were dissolved with 0.5 ml of Phosphate buffered saline (PBS) containing 5 μg/ml PI and viable cells were determined by using a ...
A human lymphoblastoid cell line stably expressing a human cytochrome P450IA2 cDNA was developed. This recombinant cell line displayed P450IA2 protein and estradiol 2-hydroxylase activity, neither of which was detected in the parental cell line. The recombinant cell line was also approximately 1000- …
TY - JOUR. T1 - Effect of SXWS/WSXWS peptides on chemotaxis and adhesion of the macrophage-like cell line J774. AU - Szabõ, Rita. AU - Láng, Orsolya. AU - Láng, Júlia. AU - Illyés, Eszter. AU - Köhidai, Lászlõ. AU - Hudecz, Ferenc. PY - 2015/4. Y1 - 2015/4. N2 - WSXWS motif is a conserved amino acid sequence that is present in type I cytokine receptors. This motif that can be found both in the ligand binding chains and signal transducer molecule of the receptors with different amino acids at the position X plays a role in the receptor folding, ligand binding and signal transduction as well. Structural analysis proved that WSEWS motif of IL-6R is located in a highly accessible location in the protein. Structural properties and chemotaxis of a tetrapeptide library with SXWS sequence, where X was the 19 proteinogenic amino acids except cystein were systematically studied earlier. It has been proved that C-terminal amidation and the identity of amino acid X had a pronounced influence on ...
We have constructed a HeLa cell line that both expresses high levels of CD4 and contains a single integrated copy of a beta-galactosidase gene that is under the control of a truncated human immunodeficiency virus type 1 (HIV-1) long terminal repeat (LTR). This cell line, called CD4-LTR/beta-gal, can be used to determine quantitatively the titer of laboratory-adapted HIV strains, and the method used to do so is as sensitive as the determination of viral titers in a T-cell line by end point dilution. Using this cell line as a titer system, we calculated that HIV-1 stocks contain only one infectious particle per 3,500 to 12,000 virions. Virus derived from a molecular clone of a macrophagetropic provirus will not infect this cell line. We have also cocultivated peripheral blood lymphocyte cultures from HIV-infected individuals with the CD4-LTR/beta-gal indicator cells. In a majority of primary isolates (five of eight), including isolates from asymptomatic patients, rare virus-infected cells that can ...
Saferali A, Grundberg E, Berlivet S, Beauchemin H, Morcos L, Polychronakos C, Pastinen T, Graham J, McNeney B, Naumova AK. Cell culture-induced aberrant methylation of the imprinted IG DMR in human lymphoblastoid cell lines. Epigenetics. 2010 Jan 01; 5(1):50-60 ...
TY - JOUR. T1 - Characterization of lymphoid cell lines from murine spleen which can localize in thymus. AU - ONeill, H C. AU - De Cello, G. AU - ONeill, T J. PY - 1992/2. Y1 - 1992/2. N2 - Several murine lymphoid cell lines have been tested for specific capacity to localize in thymus. These are all continuous, cloned Radiation leukemia virus-induced cell lines which have a common phenotype resembling lymphoid stem cells or immature T cells. Since each of these cell lines has a cloning efficiency approaching 100%, the number of cells which enters thymus during a 3 h homing assay has been estimated by limit dilution cloning analysis taking into account extra-binomial variation caused by individual mice. Only two out of seven of these cell lines have been found to have this specific property. These two cell lines, 16C1 and 5C2B, have been characterized as immature lymphoid cells, bearing no rearrangement at the TCR gamma and beta loci, and having the phenotype of CD3+CD4-CD8-, immature T cells. ...
Müller glial cultures have made a significant contribution for understanding retinal pathophysiology, especially in the context of retinal degenerative diseases. Primary Müller glial cultures have several advantages, largely due to their phenotypic and functional similarity to Müller glia in vivo. There are, however, several disadvantages in using primary cultures, including limited availability, and inconsistency in experimental results, perhaps due to purity, heterogeneity across different batches, and phenotypic instability across multiple passages.14 The use of a stabilized cell line can overcome these drawbacks, although it is recommended that key results obtained from cell lines are further validated using primary cells and in vivo models. Various Müller glial cell lines, derived from rodents14-17 or humans18 have been reported. Although they all express Müller glial markers, each cell line has its own characteristics and is used to study different functions of Müller glia. For ...
Hybrid (both intra-species and inter-species) cell lines arise through intentional or nonintentional fusion of somatic cells having different origins. Hybrid cell lines can pose a problem for authentication testing to confirm cell line identity, since the results obtained may not conform to the results expected for the two parental cell types. Thus, depending on the identity testing methodology, a hybrid cell may display characteristics of one of the parental cell type or of both. In some instances, the hybrid cell line may display characteristics that are different from those displayed by either parental cell type; these differences may not necessarily indicate cellular cross-contamination. Testing should be performed as soon as possible after an intended fusion has occurred, so that a baseline reference profile is available for later comparison. In this article, we describe the various approaches that have been used for identifying hybrid cell lines and the results that might be expected when using
Stable cell lines play an important role in the study of recombinant protein preparation, antibody preparation, drug screening, gene function and other aspects. The stable cell lines can continue to grow, and express foreign gene. Changing the copy number and promoter activity can regulate the expression level of exogenous gene in target cells, which makes it increase, decrease, or equal to the endogenous gene expression amount of target cells. Insert modes of exogenous genes can be divided into random insertion or fixed position insertion.. Creative Biogene is now providing stable cell lines construction service for the majority of researchers. We use the most advanced technology, to provide a stable cell line with high quality for you. The construction service can use CHO, HEK293, Hela, NIH3T3 and other tools, or use specific cells designed by customers. From cloning construction, cell line establishment, gene amplification, monoclonal screening, cell line identification, functional research, ...
However, it is not easy to develop a stable cell line that maintains ideal growth and productivity characteristics. There are many factors that can influence the bioproduction level including vector expression elements, vector delivery efficiency, screening system etc. This imposes very significant costs and time in the development of a desirable bioproduction stable cell line.. Leading Capability. As an unmatched global provider of life science services and products, Creative Biogene offers comprehensive services for generating bioproduction stable cell lines. Our service scope covers all steps of stable cell line generation, from the first gene synthesis to the final delivery of high-producing cell clones.. To improve the production rate of recombinant proteins and the selection efficiency, combined with our genome editing platform, Creative Biogene has developed our own mammalian cell expression systems including cells based on glutamine synthetase (GS) selection and cells based on ...
Velagapudi, Ravikanth, Olajide, Olumayokun A and Aderogba, Mutallib A. (2014) Tiliroside Produced Anti-Neuroinflammatory Effects Through Interference With NF-κB And MAPK Signalling In LPS+ IFN-γ Stimulated BV-2 Microglia. pA2 Online. ISSN 1741-1149 ...
De la chapelle, A; Fantoni, A; and Marks, P A., Differentiation of mammalian somatic cells, dna and hemoglobin synthesis in fetal mouse yolk sac erythroid cells. (1969). Subject Strain Bibliography 1969. 1375 ...
Fig. 1. Acentriolar cell lines establishment and characterization. (A) Different 2-3-week-old primary cultures obtained from dissociated embryos. Cells present various morphologies. Scale bar: 50 µm. (B) Representation of the survival of primary cultures. Most of primary cultures differentiate and stop to grow but some of them spontaneously immortalize. 11 permanent cell lines have been obtained. (C) FACS profiles of a young mixed line (line 23) before (blue) and after (green) heat shock. X-axis: intensity of fluorescence. Y-axis: number of cells. The narrowing of the fluorescence peak reflects the selection against hs-hid genotypes. (D) PCR genotyping of the 11 cell lines. Two couples of primers (AB or BC, see Materials and Methods) allow detection of WT or mutant DSas-4 alleles. From this analysis 6 lines are pure DSas-4−/− (as line 131) and 5 lines are DSas-4+/− (as line 110). (E) Immunofluorescence of control (upper image) and DSas-4−/− no. 70 (lower image) mitotic cells stained ...
HLA A*0201 WT 1 complex Protein Human HEK293 His best suppliers; HLA A*0201 WT 1 complex Protein Human HEK293 His best sources; HLA A*0201 WT 1 complex Protein Human HEK293 His best vendors; HLA A*0201 WT 1 complex Protein Human HEK293 His protocol; HLA A*0201 WT 1 complex Protein Human HEK293 His citations; HLA A*0201 WT 1 complex Protein Human HEK293 His publications; HLA A*0201 WT 1 complex Protein Human HEK293 His papers - Labshake
RNA interference is an evolutionary conserved immune response mechanism that can be used as a tool to provide novel insights into gene function and structure. The ability to efficiently deliver small interfering RNA to modulate gene expression in vivo may provide new therapeutic approaches to currently intractable diseases. In vitro, siRNA targeting IL-12p40 was delivered to the murine macrophage cell line (J774A.1) encapsulated in a liposome with an IL-12 inducing agent (LPS/IFN-γ) over a number of time points. Controls included a variety of non-target specific siRNA reagents. Supernatants were analyzed for cytokine production while the cells were removed for mRNA profiling. In vivo, siRNA-targeting IL-12p40 was delivered to the murine peritoneal cavity in a therapeutic fashion, after endotoxin (LPS) challenge. Cells from the peritoneal cavity were removed by lavage and analyzed by flow cytometry. Levels of IL-12 present in lavage and in serum were also examined by ELISA. In this report, we show that
In: Proceedings of a symposium on continuous cell lines as substrates for biologicals, Arlington, Virginia USA, May 1988. Basel: Karger, 1989;113-24 (Developments in biological standardization; 70 ...
Shirley, NY-Profacgen, a world-leading custom protein service provider for a broad spectrum of industrial and academic clients with a commitment to delivering high-quality data and customer services, is pleased to update its stable cell line construction services. Scientists in the field of protein expression now have access to Profacgens latest stable cell line construction service version supported by cutting-edge facilities.. When large amount of recombinant protein is required, stable expression line construction is recommended for reproducible and cost-effective protein production. Generally, stable cell line construct will take 3 steps: delivery of the target gene into the host cell, screening of stable expression clones, and stability test. For efficient gene delivery and to achieve high integration rate, the company uses lentiviral vector. Limited dilution and antibiotics screening are then applied to select for high-yield, genetically homogenous stable expression monoclones. When ...
DC-SIGN cell surface distribution during monocyte-derived DC development. DC-SIGN binding activity was monitored during development of monocyte-derived DCs. As
The Antibody Labs proprietary cell line development technology enables drug developers to move seamlessly from preclinical discovery to manufacture of the biologic for clinical testing. BESTcell clonal Chinese hamster ovary cell lines can be rapidly generated to enable preclinical testing of multiple biologic drug candidates. After selection of the final candidate, the respective cell line can be used to manufacture master cell banks. This revolutionary approach shortens timelines and reduces the reproducibility risk associated with changing the source of the biologic during research and development.
TY - JOUR. T1 - Isolation of myoblastic, fibro-adipogenic, and fibroblastic clonal cell lines from a common precursor and study of their requirements for growth and differentiation. AU - Darmon, Michel. AU - Serrero, Ginette. AU - Rizzino, Angie. AU - Sato, Gordon. N1 - Funding Information: We thank Dr H. Jakob for the gift of the T984 cell line and for the stimulatingd iscussionswhich led to this study and also Drs H. E&en, J.-F. Nicolas, M. H. But and F. Jacob for stimulatingd iscussions. We thank Dr R. Bloch for his assistancei n performing experimentsw ith rhodamine-labeleda BT and Drs J. Orly, D. McClure, and D. Barnes for their valuable help during this work. We thank Dr D. Weinstein for helu in oreoarine delioidated and delioooroteinized sera and-D; S. Cohenfor the gift of high molecular weight EGF. These experimentsw ere conductedd ur-ingthe tenure of an ACS-Eleanor Roosevelt lnter- national Cancer Fellowship awarded by the Intema-tional Union Against Cancer to M. D. USPHS-CA 19731a nd ...
Cell lines are an invaluable scientific tool. They allow us to dissect the internal workings of tissues in a controlled environment without the ethical implications of working with whole organisms. Starting with the first successful immortal cell line HeLa, the number of available cell lines has since diversified into a plethora of options. Just like model […]. The post Top 5 of the most commonly used cell lines! appeared first on Bitesize Bio.. ...
Sialyl Transferase-EGFP transfected HeLa cells demonstrating the redistribution of the Golgi Apparatus to the Endoplasmic Reticulum upon treatment wit...
TY - JOUR. T1 - Functional analysis of an established mouse vascular endothelial cell line. AU - Nishiyama, Tatsuaki. AU - Mishima, Kenji. AU - Ide, Fumio. AU - Yamada, Koichi. AU - Obara, Kumi. AU - Sato, Aki. AU - Hitosugi, Noriko. AU - Inoue, Hiroko. AU - Tsubota, Kazuo. AU - Saito, Ichiro. PY - 2007/2/1. Y1 - 2007/2/1. N2 - Background: In vitrostudies using cell lines are useful for the understanding of cellular mechanisms. The purpose of our study is to develop a new immortalized aortic vascular endothelial cell (EC) line that retains endothelial characteristics and can facilitate the study of ECs. Methods: A mouse aortic vascular EC line (MAEC) was established from p53-deficient mouse aorta and cultured for over 100 passages. The expression of endothelial markers was assessed, and the function of this cell line was analyzed by tube formation and binding assays. Results: MAEC retained many endothelial properties such as cobblestone appearance, contact-inhibited growth, active uptake of ...
...ANN ARBOR Mich. The University of Michigans first human embryonic s...The line known as UM4-6 is a genetically normal line derived in Oct... This is significant because acceptance of these cells on the registr... It now makes the line available to researchers who can apply for fede...,U-M,human,embryonic,stem,cell,line,placed,on,national,registry,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
Last week, we finished our Ask the Expert Discussion on Cell Line Authentication. Inadvertent cell line contamination is a serious concern for many researchers. Failure to monitor cell lines for contamination may result in compromised data. Current estimates are that 18% to 36% of all active cell lines are either cross-contaminated or misidentified.1
Endothelial cells are believed to play an important role in response to virus infection. Our previous microarray analysis showed that H9N2 virus infection and inactivated viral particle inoculation increased the expression of interferon-inducible transmembrane protein 1 (IFITM1) in human umbilical vein endothelial cells (HUVECs). In present study, we deeply investigated the expression patterns of IFITM1 and IFITM1-mediated antiviral response induced by H9N2 virus infection and inactivated viral particle inoculation in HUVECs. Epithelial cells that are considered target cells of the influenza virus were selected as a reference control. First, we quantified the expression levels of IFITM1 in HUVECs induced by H9N2 virus infection or viral particle inoculation using quantitative real-time PCR and western blot. Second, we observed whether hemagglutinin or neuraminidase affected IFITM1 expression in HUVECs. Finally, we investigated the effect of induced-IFITM1 on the antiviral state in HUVECs by siRNA and
The Infectious Disease Cell Line Microarray consists of either 2 or 9- 2mm cores of formalin-fixed paraffin-embedded infected cell lines, which were assembled in array fashion to allow multiplex molecular pathology analysis and validation of infectious disease reagents, or to be used as controls for Immunohistochemistry and/or in situ hybridization (CISH and FISH) applications.The 9-core Infectious Disease Cell Line Microarray consists of all positive (+) cell lines listed below, as well as one negative (-) core. The 2-core Infectious Disease Cell Line Microarrays include one positive (+) and one negative (-) core.. ...
Hello, I am working with HepG2-C3A hepatocyte cell line. My goal is to transfect these cells with GFP reporter plasmids and establish stable cell lines. The plasmids have Neomycin resistance gene in them. Can somebody please help me with these queries? 1. Does anybody have a set protocol that describes the procedure starting from trancfecting cells to performing gene expression studies using stably transfected cells? 2. Do the cells usually have a different generation time after stable transfection? 3. Do you have to screen for clones that behave as close as the wild type? If so, how many do you have to screen? 4. How long does this whole procedure take? Cheers, Murali Vemula ...
Hello all- I am in need of a good all around human endothelial cell line for use in viral transformation studies, as well as studies of certain viral genes. I have looked through some of the thousands at ATCC, but most have been derived from leukemias, etc. I would prefer one that is normal (as normal as a culturable cell line can be). The big restriction is that I am looking for an endothelial line. Thanks in advance. Kajetan -------------- next part -------------- A non-text attachment was scrubbed... Name: Kajetan-Groicher.vcf Type: text/x-vcard Size: 427 bytes Desc: Card for Kajetan H. Groicher Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19991110/240b4f7f/Kajetan-Groicher.bin ...
TGF-β isoforms are important signalling molecules in wound repair in the skin. Transforming growth factor β3 (TGF-β3) has been implicated in scarless healing. In both animal and human models the application of exogenous TGF-β3 causes a reduction in the inflammatory response and improves the architecture of the neodermis. Research into the influence of TGF-β on scarring has tended to focus on fibroblasts. However, keratinocytes play a major role in scarring both indirectly, as a result of their influence over the behaviour of fibroblasts and also by directly influencing wound contraction. Thus, experiments were carried out to investigate the influence of TGF-β3 on the behaviours of a keratinocyte cell line (HaCaT). Incubation with TGF-β3 increased cell spreading and appeared to reduce cell-surface contacts indicated by both SPR imaging and a detachment assay. TGF-β3 also caused a decreased cell alignment response to microcontact printed protein patterns, in part due to the deposition of ...
Stable recombinant cell line expressing the Muscarinic, M1 receptor. Human recombinant, in CHO-K1 host cell. We provide: Two vials of the recombinant cell line as frozen cells; Detailed product information including sequence, cell line properties, culture conditions, pharmacological properties of the recombinant receptor in binding and functional assays (cAMP). In addition, for total reassurance, we will provide up to two free replacements within in a 2 year period should you accidently lose your cell line ...
BALB/MK keratinocyte cell line cultured in a defined medium. b Overexpressed. c FRSK cell line. d PAM212 cell line. c HPK1A cell line.. duced. Approximately 106-107 cells are injected in 0.1 ml PBS into the interscapular region of the host.19 Most malignant keratinocytes can grow subcutaneously, whereas papilloma or benign tumor cells do not form tumors94 (Table III).. 91 D. A. Greenhalgh and S. H. Yuspa, Mol Carcinog. 1, 134 (1988).. 92 C. M. Kim, J. Vogel, G. Jay, and J. S. Rhim, Oncogene 7, 1525 (1992).. 93 E. Finzi, A. Kilkenny, J. E. Strickland, M. Balaschak, T. Bringman, R. Derynck, S. Aaron-son, and S. H. Yuspa, Mot. Carcinog. 1, 7 (1988).. 94 J. E. Strickland, D. A. Greenhalgh, A. Koceva-Chyla, H. Hennings, C. Restrepo, M. Balaschak, and S. H. Yuspa, Cancer Res. 48, 165 (1988).. Was this article helpful?. ...
Table of Contents. Cell Lines Derived from Human Tumors for Vaccine Manufacture. 1. Introduction……………………………………………………………………………..……………………………4. 2. Background………………………………………………………….………………………………………………..4. 2.1 History of Cell Substrates for Viral Vaccine Manufacture in the U.S.: Primary,. Diploid, and Tumorigenic Cells……………………………….…………………………………………………5. 2.2 Cell Substrates Derived from Human Tumors Proposed for Manufacture of. Viral vaccines……………………………………………………………………………………………………………6. 3. Considerations Regarding the Use of Tumor-derived Cell Lines……..…………………………8. 3.1 Mechanism of Tumorigenesis and Considerations for Characterization of ...
Fingerprint Dive into the research topics of Techniques for purifying L-cell karyoplasts with minimal amounts of cytoplasm. Together they form a unique fingerprint. ...
A. Left: A consolidated overview of PLSR‐derived variable importance in the projection (VIP) scores mapped onto a simple schematic of immediate‐early signaling, indicating the fraction of 10 studied cell lines in which early (1-10 h) or late (24-48 h) up‐ or down‐regulation of each of the cell state markers and phospho‐proteins is predictive of response to RAF/MEK inhibitors (72‐h viability). Up‐regulation or down‐regulation of signals with VIP score ,1 is shown. Right: Correlation between up‐ and down‐regulation of selected pathways in response to RAF/MEK inhibition across the 10 studied cell lines. Correlations between signaling changes in response to RAF/MEK inhibition were evaluated based on pairwise Spearmans correlation between VIP scores for selected phospho‐proteins at 24 and 48 h post‐treatment across the 10 studied cell lines. P‐values ,0.05 were considered significant. ...
Historically, the analysis of DNA replication in mammalian tissue culture cells has been limited to static time points, and the use of nucleoside analogues to pulse-label replicating DNA. Here we characterize for the first time a novel Chromobody cell line that specifically labels endogenous PCNA. By combining this with high-resolution confocal time-lapse microscopy, and with a simplified analysis workflow, we were able to produce highly detailed, reproducible, quantitative 4D data on endogenous DNA replication. The increased resolution allowed accurate classification and segregation of S phase into early-, mid-, and late-stages based on the unique subcellular localization of endogenous PCNA. Surprisingly, this localization was slightly but significantly different from previous studies, which utilized over-expressed GFP tagged forms of PCNA. Finally, low dose exposure to Hydroxyurea caused the loss of mid- and late-S phase localization patterns of endogenous PCNA, despite cells eventually completing S
We describe a high, synergistic induction of IDO1 expression by the demethylating drug zebularine and IFN-γ in the human monocytic cell line THP-1. ► The ...
Webcat Plus: Development of membrane excitability during the in vitro differentiation of a clonal mouse myogenic cell line, 博士論文;博士論文
Dr. Barron: So, as I mentioned previously, there are two main approaches. Ideally, an industry would use this technology before going through regulatory submission. So, with a new cell line making a particular product of interest, a cell line would be manipulated stably by using something like the technology I just described. For instance, over expression sponge that would target the microRNA of interest, or indeed, over expressing a short hairpin that will mimic the microRNA of interest if over expression is desired. So, that forms a stable cell line that can act as the parent, into which the product gene or the transgene is placed and that would go through the normal regulatory process ...
This webinar will discuss the evolving role of Cell Lines and Cell Culture and their usefulness as models of physiological function and disease. The webinar will review the depth, breadth and best use of currently available Cell Lines. The role played by Cell Lines in ensuring that the output of future research continues to be valid and credible will also be evaluated. This is the first of a three part webinar series focused on delivering a broad picture of the role of cell lines as models in scientific research, the various strategies and approaches available.
CAMBRIDGE, MA and SEATTLE, WA, October 3, 2017-BlueRock Therapeutics and Universal Cells Inc. today announced that they have entered into a collaboration and license agreement to create induced pluripotent stem (iPS) cell lines useful for the manufacture of allogeneic cellular therapies.. BlueRock is at the cutting edge of the cell therapy field and our collaboration with the company represents an important step in their efforts to develop off-the-shelf cell therapies for degenerative diseases, said Claudia Mitchell, Ph.D., CEO of Universal Cells. We are honored that our technology will support the advancement of BlueRocks cell therapies.. Under the terms of the agreement, BlueRock and Universal Cells will collaborate to engineer iPS cell lines for clinical use. The agreement grants BlueRock commercial rights to use the engineered cell lines for undisclosed therapeutic applications.. This partnership with Universal Cells provides us with an important technology useful to advance ...
Applied StemCell offers a comprehensive custom service for lentiviral-based Stable Cell Line Generation for genetically engineering sensitive cells lines.