After long controversy, it is now recognized that essentially all neurodegenerative diseases have inflammatory components [1]. Many other neurological disorders are exacerbated by inflammation, including the progressive impairment following stroke or traumatic brain injury (TBI). It has been known that levels of circulating cell-derived microparticles (MP) are generally increased in inflammatory states, making them useful as biomarkers. More recently, certain species of MP or exo- somes have been implicated as causative agents in neuroinflammation, to be reviewed.. L.L. Horstman, BS • W. Jy, PhD (*) • Y.S. Ahn, MD. University of Miami, Department of Medicine (Wallace H. Coulter Platelet Laboratory), 1600 NW 10TH Ave., Mail Code R36A, Miami, FL 33136, USA e-mail: This email address is being protected from spam bots, you need Javascript enabled to view it © Springer International Publishing AG 2017. A. Minagar, J.S. Alexander (eds.), Inflammatory Disorders of the Nervous System, Current ...
0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...
TY - JOUR. T1 - Phenotypic assessment of endothelial microparticles in patients with heart failure and after heart transplantation. T2 - Switch from cell activation to apoptosis. AU - Garcia, Santiago. AU - Chirinos, Julio. AU - Jimenez, Javier. AU - Del Carpio Muñoz, Freddy. AU - Canoniero, Mariana. AU - Jy, Wenche. AU - Jimenez, Joaquin J. AU - Horstman, Lawrence. AU - Ahn, Yeon. PY - 2005/12/1. Y1 - 2005/12/1. N2 - Background: Endothelial microparticles (EMPs) are sub-microscopic membrane vesicles that are shed from the surface of endothelial cells during activation, injury and/or apoptosis. Endothelial cells release phenotypically and quantitatively distinct endothelial microparticles (EMPs) in activation and apoptosis. Therefore, the phenotypic assessment of EMPs can provide useful information reflecting the nature of endothelial injury. We tested the hypothesis that heart transplantation (HT) modifies the pattern of endothelial injury seen in patients with congestive heart failure (CHF). ...
Acute HIV-1 infection results in dysregulated immunity, which contributes to poor control of viral infection. DCs are key regulators of both adaptive and innate immune responses needed for controlling HIV-1, and we surmised that factors elicited during acute HIV-1 infection might impede DC function. We derived immature DCs from healthy donor peripheral blood monocytes and treated them with plasma from uninfected control donors and donors with acute HIV-1 infections. We found that the plasma from patients with HIV specifically inhibited DC function. This suppression was mediated by elevated apoptotic microparticles derived from dying cells during acute HIV-1 infection. Apoptotic microparticles bound to and inhibited DCs through the hyaluronate receptor CD44. These data suggest that targeting this CD44-mediated inhibition by apoptotic microparticles could be a novel strategy to potentiate DC activation of HIV-specific immunity.
TY - JOUR. T1 - Circulating endothelial microparticles as a measure of early lung destruction in cigarette smokers. AU - Gordon, Cynthia. AU - Gudi, Kirana. AU - Krause, Anja. AU - Sackrowitz, Rachel. AU - Harvey, Ben Gary. AU - Strulovici-Barel, Yael. AU - Mezey, Jason G.. AU - Crystal, Ronald. PY - 2011/7/15. Y1 - 2011/7/15. N2 - Rationale: There is increasing evidence thatemphysemais associated with primary loss of pulmonary capillary endothelium. Plasma levels of endothelial microparticles (EMPs), small vesicles released from activated or apoptotic endothelial cells, are elevated in vascularrelated disorders. Objectives: To evaluate whether plasma EMP levels are elevated in smokers with early lung destruction as assessed by normal spirometry but reduced diffusing capacity of the lung for carbon monoxide (DLCO). Methods: Lung health was assessed by pulmonary function tests (PFTs: spirometry, total lung capacity, DLCO) and chest X-ray; smoking status was assessed by urine nicotine and ...
TY - JOUR. T1 - C-reactive protein induces release of both endothelial microparticles and circulating endothelial cells in vitro and in vivo. T2 - Further evidence of endothelial dysfunction. AU - Devaraj, Sridevi. AU - Kumaresan, Pappanaicken R.. AU - Jialal, Ishwarlal. PY - 2011/12. Y1 - 2011/12. N2 - BACKGROUND: Inflammation is pivotal in atherosclerosis. A key early event in atherosclerosis is endothelial dysfunction. C-reactive protein (CRP), the prototypic marker of inflammation in humans, is a risk marker for cardiovascular disease, and there is mounting evidence to support its role in atherothrombosis. CRP has been shown to promote endothelial dysfunction both in vitro and in vivo. Emerging biomarkers of endothelial dysfunction include circulating endothelial cells (CECs) and endothelial microparticles (EMPs). However, there is a paucity of data examining the effect of CRP on CEC and EMP production in vitro and in vivo. METHODS: In this report, we treated human aortic endothelial cells ...
The results of these studies demonstrate that calcineurin inhibitors cause endothelial cells to increase the number of microparticles released into the circulation from the cell surface. Calcineurin inhibitors also alter the composition of the microparticles such that they become complement activating. We found that alternative pathway-deficient mice are protected from CsA-induced renal and vascular injury. We also found that endothelial microparticles cause injury of unmanipulated endothelial cells in vitro, and they cause mesangial proliferation and complement activation when passively transferred into wild-type mice in vivo. These results demonstrate that CsA-induced endothelial microparticles can cause bystander injury of endothelial cells, and they promote glomerular complement activation and mesangial expansion. Furthermore, preliminary data from human transplant patients indicate that treatment of these patients with tacrolimus is also associated with generation of endothelial ...
Maturation (MP) or polishing ponds are widely used throughout the world as a final or tertiary wastewater treatment process for effluent quality improvement from secondary biological treatment systems, including FPs. MPs can be expected to reduce total suspended solids (TSS), ammonia, nitrate and phosphorus levels in effluent, but the primary function of MPs in most parts of the world is the removal of pathogenic organisms. A number of mechanisms in MPs are responsible for pathogen reduction, including settlement, natural die off (a function of time and temperature), elevated pH, UV radiation and predation. The design, size and number (usually in series) of MPs are typically based on the desired bacteriological quality of the final effluent. MPs are typically designed at the same depth as facultative ponds (2 to 5 feet (0.7 to 1.5 m)). Retention times in MPs are typically designed at 10 to 14 days for pathogen reduction; however, shorter retention times are required for TSS removal (four to five ...
Microparticles are membrane vesicles with procoagulant and proinflammatory properties released during cell activation or apoptosis. Microparticles from monocytes have been implicated in atherosclerosis and vascular inflammation, but their direct effects on endothelial cells are not completely elucidated. The present study was designed to dissect the signaling pathways of monocytic microparticles in endothelial cells with respect to both NO pathway and reactive oxygen species. Microparticles were produced by treatment of human monocytic cell line THP-1 with the apoptotic agent VP-16. Human endothelial cells were treated with monocytic microparticles and then, we studied their effects on nitrosative and oxidative stresses. Incubation of human endothelial cells with microparticles enhanced the production of NO without affecting superoxide anions generation. Microparticles did not affect endothelial NO synthase expression and its phosphorylation. Interestingly, microparticles decreased caveolin-1 ...
Microparticles are a heterogeneous population of membrane-coated vesicles which can be released from virtually all cell types during activation or apoptosis. Release occurs from the cell surface in an exogenous budding process involving local rearrangement of the cytoskeleton. Given their origin, these particles can be identified by staining for cell surface markers and annexin V. As shown in in vitro studies, microparticles may represent a novel subcellular element for intercellular communication in inflammation. Thus, microparticles can transfer chemokine receptors and arachidonic acid between cells, activate complement, promote leukocyte rolling and stimulate the release of pro-inflammatory mediators. Under certain conditions, however, microparticles may also exert anti-inflammatory properties by inducing immune cell apoptosis and the production of anti-inflammatory mediators. Microparticles may play an important role in the pathogenesis of rheumatologic diseases as evidenced by their elevation in
Simoncini S, Njock MS, Robert S, Camoin-Jau L, Sampol J, Harlé JR, Nguyen C, Dignat-George F, Anfosso F. Circ Res. 2009 Apr 24 ;104 (8):943-51 (...)
Oxidative Medicine and Cellular Longevity is a unique peer-reviewed, Open Access journal that publishes original research and review articles dealing with the cellular and molecular mechanisms of oxidative stress in the nervous system and related organ systems in relation to aging, immune function, vascular biology, metabolism, cellular survival and cellular longevity. Oxidative stress impacts almost all acute and chronic progressive disorders and on a cellular basis is intimately linked to aging, cardiovascular disease, cancer, immune function, metabolism and neurodegeneration. The journal fills a significant void in todays scientific literature and serves as an international forum for the scientific community worldwide to translate pioneering
Microparticles (MPs) are fragments of cell membranes released from stimulated or apoptotic cells that have long been considered innocent remnants of cell destruction. Evidence is accumulating, however, that the circulating and tissue-derived MPs may elicit a strong thrombogenic and inflammatory response (1). In addition to MPs, activated cells can also release smaller vesicle-denominated exosomes that are relatively unexplored in the research area of vascular biology (2).. Circulating MPs are being explored as a potential source for biomarker discovery. Platelet MPs have been associated with clinically evident atherosclerotic disease in diabetes type II patients (3), and leukocyte-derived MPs are predictive of subclinical atherosclerosis in asymptomatic patients (4).. Experimental and clinical data consistently point to a causal role for MPs in atherosclerosis development and progression. Recent reports revealed their contribution to hemostatic and inflammatory responses, vascular remodeling and ...
Background: Early and non-invasive biomarkers of kidney damage are needed to identify hypertensive patients at risk for kidney damage. Urinary micropaticles (UMPs) have gained significant attention as potential novel biomarkers for kidney damage, and have already been identified in pre-albuminuric diabetic glomerular injury. These vesicles are less than 1 micron in size and carry markers of the parent cell. We hypothesized that podocyte derived UMPs are elevated in angiotensin II-induced hypertension (HTN). Methods: Primary podocytes were isolated and grown in culture. Wild-type mice were treated with AII (400ng/kg/min) via mini-osmotic pumps. Untreated WT mice served as controls. 24 hour urines were collected after 5 days of AII treatment. Enumeration and phenotyping of MPs was done of podocyte culture supernatant and urine. Podocalyxin (Pcal), podoplanin (Ppla) and annexin 5 (AV) were used as surface markers.. Result: Pcal and Ppla positive MPs as well as AV positive and negative MPs were ...
CA153 : The instrument used is the Roche Cobas. The Roche cancer antigen 15-3 (CA 15-3) method is a sandwich electrochemiluminescence immunoassay that employs a biotinylated monoclonal CA 15-3-specific antibody and a monoclonal CA 15-3-specific antibody. CA 15-3 in the automatically prediluted specimen reacts with both the biotinylated monoclonal CA 15-3-specific antibody (mouse) and the monoclonal CA 15-3-specific antibody (mouse) labeled with a ruthenium complex, forming a sandwich complex. Streptavidin-coated microparticles are added and the mixture is aspirated into the measuring cell where the microparticles are magnetically captured onto the surface of the electrode. Unbound substances are then removed with ProCell. Application of voltage to the electrode induces the chemiluminescent emission, which is then measured.(Package insert: Roche CA 15-3 reagent, Roche Diagnostic Corp., Indianapolis, IN 2010-09, V14)
BOSTON, Massachusetts (PNN) - March 28, 2017 - A team of scientists at the Boston Childrens Hospital have invented what is being considered one the greatest medical breakthroughs in recent years. They have designed a microparticle that can be injected into a persons bloodstream that can quickly oxygenate his or her blood. This will even work if the ability to breathe has been restricted, or even cut off entirely.. This finding has the potential to save millions of lives every year. The microparticles can keep an object alive for up to 30 minutes after respiratory failure. This is accomplished through an injection into the patients veins. Once injected, the microparticles can oxygenate the blood to near normal levels. This has countless potential uses as it allows life to continue when oxygen is needed but unavailable. For medical personnel, this is just enough time to avoid risking a heart attack or permanent brain injury when oxygen is restricted or cut off to patient.. Dr. John Kheir, who ...
2015. Ghelani H, Razmovski-Naumovski V, Kyada AV, Parekh HS, Li GQ, Nammi S. Myoglobin and low density lipoprotein glycation in cardiac complications of diabetes: Current perspectives and therapeutic options. Indian J Med Res. 2015. In Press. Jadeja R, Devkar RV, Nammi S. Hepatoprotective potential of herbal medicine. Evid-Based Compl Alt. [Editorial]. 2015;2015.. Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Chan K, Grau GE, et al. Curcumin reduces tumour necrosis factor-enhanced annexin V-positive microparticle release in human vascular endothelial cells. J Pharm Pharm Sci. 2015;18(4):424-33.. Thounaojam MC, Nammi S, Jadeja R. Natural products for the treatment of obesity, metabolic syndrome, and type 2 diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/392681.. Yang WC, Chang CLT, Li CR, Nammi S, Cho WCS. Complementary and alternative medicine for diabetes 2014. Evid-Based Compl Alt. [Editorial]. 2015;2015. DOI: 10.1155/2015/685248.. 2014. Li Y, Tran VH, Kota BP, ...
TY - JOUR. T1 - Modification of molecular conformation of spray-dried whey protein microparticles improving digestibility and release characteristics. AU - Ye, Qianyu. AU - Woo, Meng Wai. AU - Selomulya, Cordelia. PY - 2019/5/15. Y1 - 2019/5/15. N2 - This study reports on the preparation of riboflavin-loaded whey protein isolate (WPI) microparticles, using desolvation and then spray drying. Ethanol desolvation led to the exposure of embedded hydrophobic amino acids of WPI to riboflavin, facilitating the formation of riboflavin-WPI complexes. The extent of desolvation and cross-linking influenced the morphology of the spray-dried microparticles, while the moisture content of microparticles decreased with desolvation and increased with crosslinking. The modification of WPI conformation upon desolvation could be retained in the dry state via spray drying. The gastric resistance, release site and release characteristics of microparticles were readily adjusted by varying the ethanol and calcium ion ...
Overall research in my lab explores the molecular mechanisms of cellular signal transduction, with several related foci: 1) intercellular signaling in platelet-tumor interactions, including the contributions of Ras family small G proteins; 2) the contributions of protein trafficking to the signaling and oncogenic functions of Ras proteins; 3) Ras signaling in platelets, regulating thrombosis and hemostasis. My long term goal is to reach a new understanding of cellular information processing and its contributions to signal responses and to translate these findings into pre-clinical approaches.. 1. Intercellular signaling in platelet-tumor interactions.. We recently discovered that platelet microparticles - small extracellular vesicles released by blood platelets into plasma - infiltrate solid tumors by means of the unusual permeability of tumor vasculature, whereupon the platelet microparticles anchor to tumor cells and transfer genetic information in the form of platelet-derived microRNAs ...
VEGF/VEGFR inhibitors, used as anti-angiogenic drugs to treat cancer, induce severe hypertension. Molecular mechanisms are unclear, but nitric oxide (NO) and oxidative stress may be involved. We questioned whether reactive oxygen species (ROS) and Ang II also play a role in VEGF inhibitor-induced vascular dysfunction. Human microvascular endothelial cells (HMECs) were stimulated with vatalanib (VAT-VEGFR inhibitor) and gefitinib (GEF-EGFR inhibitor) in the absence/presence of Ang II. Activation of eNOS and MAPKs were assessed by immunoblotting. Antioxidant enzyme mRNA was analysed by qPCR. Endothelial microparticles, biomarkers of endothelial damage, tend to increase in subjects treated with VEGFR inhibitors. Phosphorylation of eNOS (28.3% ± 7.1) was decreased by VAT (p , 0.05). VAT decreased mRNA expression of Nox4 (0.5 ± 0.2) and H2O2-regulating antioxidants enzymes such as catalase (0.4 ± 0.1) and glutathione peroxidase (0.4 ± 0.1), while increased mRNA levels of Nox5 (3.35 ± 1.1) (p , ...
Background: Neopetrosiamide A (NeoA) is a 28-amino acid tricyclic peptide originally isolated from a marine sponge as a tumor cell invasion inhibitor whose mechanism of action is unknown. Methodology/Principal Findings: We show that NeoA reversibly inhibits tumor cell adhesion, disassembles focal adhesions in pre-attached cells, and decreases the level of beta 1 integrin subunits on the cell surface. NeoA also induces the formation of dynamic, membrane-bound protrusions on the surface of treated cells and the release of membrane-bound vesicles into the culture medium. Proteomic analysis indicates that the vesicles contain EGF and transferrin receptors as well as a number of proteins involved in adhesion and migration including: beta 1 integrin and numerous alpha integrin subunits; actin and actin-binding proteins such as cofilin, moesin and myosin 1C; and membrane modulating eps15 homology domain (EHD) proteins. Surface labeling, trafficking inhibition, and real-time imaging experiments all ...
M. ABBAS, L. JESEL, C. AUGER, L. AMOURA, N. MESSAS, G. MANIN, C. RUMIG, A.J. LEON-GONZALEZ, T.P. RIBEIRO, G.C. SILVA, R. ABOU-MERHI, E. HAMADE, M. HECKER, Y. GEORG, N. CHAKFE, P. OHLMANN, V.B. SCHINI-KERTH, F. TOTI & O. MOREL. Endothelial microparticles from acute coronary syndrome patients induce premature coronary artery endothelial cells ageing and thrombogenicity: role of the Ang II/AT1 receptor/NADPH oxidase-mediated activation of MAPKs and PI3-kinase pathways. Circulation 135, 280-296, 2017 - IF 17.20 - ...
Pitha J, Dorazilova Z, Melenovsky V, Kralova Lesna I, Stavek P, Stepankova J, Urban M, Maly J, Netuka I. The impact of left ventricle assist device on circulating endothelial microparticles - pilot study. Neuro Endocrinol Lett. 2012 Jan; 33(Suppl 2): 68-72 ...
A beam of accelerated microparticles of substantially uniform velocity is passed transversely through an intense pulsed laser beam. In traversing the laser beam, the microparticles are thermally evapo
TGF-β3 is enzymatically immobilized by transglutaminase-2 action to poly(L-lactic acid) microparticles coated with collagen II. Microparticles are then encapsulated with stem cells inside liquified spherical compartments enfolded with a permselective shell through layer-by-layer adsorption. Magnetic nanoparticles are electrostatically bound to the multilayered shell, conferring magnetic-response ability. The goal of this study is to engineer a closed environment inside which encapsulated stem cells would undergo a self-regulated chondrogenesis. To test this hypothesis, capsules are cultured in chondrogenic differentiation medium without TGF-β3. Their biological outcome is compared with capsules encapsulating microparticles without TGF-β3 immobilization and cultured in normal chondrogenic differentiation medium containing soluble TGF-β3. Glycosaminoglycans quantification demosntrates that similar chondrogenesis levels are achieved. Moreover, collagen fibrils resembling the native ...
Summary of Facts and Submissions. I. This appeal is from the interlocutory decision of the Opposition Division concerning maintenance of European patent No. 1 328 683 in amended form on the basis of the then pending main request. The independent Claim 1 reads:. 1. A process for making paper or paper board comprising forming a cellulosic suspension, flocculating the suspension, draining the suspension on a screen to form a sheet and then drying the sheet,. characterised in that. the suspension is flocculated using a flocculating system comprising a siliceous material and organic microparticles which have an unswollen particle diameter of less than 750 nanometers,. wherein a further flocculating material is included into the cellulosic suspension before adding the polymeric microparticles and siliceous material,. and in which the flocculating material is cationic and is a natural or synthetic polymer,. in which the microparticles are made from anionic copolymers comprising from 0 to 99 parts, by ...
A novel microparticle preparation comprising microparticles of a polymer which contain a drug and are coated with a film of an agent for preventing aggregation of the microparticles is disclosed. The preparation is produced by spraying a solution of a polymer containing a drug and an aqueous solution of an agent for preventing aggregation of the microparticles separately from different nozzles and contacting them with each other in a spray dryer.
The argument that we must do everything a Minister demands because he has been democratically chosen does not stand up to close inspection. MPs are not chosen by the people - they are chosen by their local constituency parties: thirty-five men in grubby raincoats or thirty-five women in silly hats. The further selection process is equally a nonsense: there are only 630 MPs and a party with just over 300 MPs forms a government and of these 300, 100 are too old and too silly to be ministers and 100 too young and too callow. Therefore there are about 100 MPs to fill 100 government posts. Effectively no choice at all ...
Macrophages (MPs) are heterogeneous, multifunctional, myeloid-derived leukocytes that are part of the innate immune system, playing wide-ranging critical roles in basic biological activities, including maintenance of tissue homeostasis involving clearance of microbial pathogens. Tumor-associated MPs …
Chepkwony said that they wanted the structure of the National Parliament replicated in county assemblies so that if MPs are appointed as Ministers then MCAs are also appointed as CECs.. "In the coming months we shall hold an AGM for all MCAs and we shall combine our recommendations and hand them to the BBI taskforce," he said.. On his part, the forum chairman Ndegwa Wahome said that they were against the proposal for regional governments noting that Kenyans were currently over-represented.. Wahome who is also the Nyandarua Assembly Speaker, said that the current system should be strengthened adding that wards should be funded as one way of addressing the challenges facing Wanjiku.. ...
Thirty years for The Three Profiteers and the Parasite Peer : John Wright a writer and specialist in crime is outraged along with millions of other people that only four MPs are to be charged out of the hundreds who fiddled their expenses and stole public money John reckons we should give them all thirty y
Hospital parking charges have been slammed for placing an unfair financial burden on carers. MPs are looking to change the law to force health bosses to ex...
This petition has received 10,759 signatures. our MPS are dragging there feet on this . i have a letter from boris johnston who wants to bring in new regulations o...
POSITEK 4G Microparticle Technology is the latest generation in Nalco microparticle innovation. This new programme is designed to promote drainage and retention without causing any negative effects caused by over-flocculation. | Ecolab
Vesicles are small membrane-bound structures containing materials for export. They combine with the outer cell membrane, releasing their contents. Epithelial cells form lining layers. ...
View Notes - fulltext37 from SPECTOGRAP 545 at AIB College of Business. Nano- and Microparticles of Organic Fluorescent Dyes Self-organization and Optical Properties SUZANNE FERY-FORGUES, MOUHAMMAD
ananyo writes Rabbits with blocked windpipes have been kept alive for up to 15 minutes without a single breath, after researchers injected oxygen-filled microparticles into the animals blood. Oxygenating the blood by bypassing the lungs in this way could save the lives of people with impaired brea...
phagosome: A membrane-bound vesicle found in a cell by an inward folding of the cell membrane to hold foreign matter taken into the cell by phagocytosis.
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
An Indian-origin researcher has created tiny polymer beads that can slowly release anti-inflammatory drugs, and break down into non-toxic components.
Choosing to participate in a study is an important personal decision. Talk with your doctor and family members or friends about deciding to join a study. To learn more about this study, you or your doctor may contact the study research staff using the contacts provided below. For general information, Learn About Clinical Studies. ...
It has been thirty years since one of the authors (EJD) began a collaboration with Professor Milton Kerker at Clarkson University in Potsdam, New
Cisapid Mps in Telugu - యొక్క ఉపయోగాలు, మోతాదు, దుష్ప్రభావాలు, ప్రయోజనాలు, పరస్పర చర్యలు మరియు హెచ్చరికను కనుగొనండి - Cisapid Mps yokka upayogaalu, mothaadu, dushprabhaavaalu, prayojanaalu, praspara charyalu mariyu hechcharika
University of Delaware, Doctoral Student, Chemical Engineering, 2013 - present. National Taiwan University, B.S., Chemical Engineering, 2008 - 2012. Research Project. Cell-derived microparticles (MPs) released from budding off the cell membrane of several type of mammalian cells. These MPs have been found playing an important role in cell-cell interaction by transffering RNA, protein and lipids. Based on the characteristics that these MP are target specific, biocompatible and immunologically inert, my project focus on engineering megakaryocyte-derived MPs as vesicles for exogenous-material-transfer, and investigating the key factors that manipulate the interactions between megakaryocyte-derived MPs with hematopoietic stem cell.. Technical Skills. ...
Extracellular vesicles are a heterogeneous group of membrane-enclosed vesicles, which play an important role in intercellular communication. Increasing number of studies have shown that tumor-derived extracellular vesicles might be involved in the transfer of oncogenic cargo (proteins, lipids, messenger RNA, microRNA, non-coding RNAs and DNA) through which cancer cells could shape the tumor microenvironment and influence tumor progression. Nasopharyngeal carcinoma-derived extracellular vesicles have also reported to facilitate tumor proliferation, metastasis and immune escape. Moreover, nasopharyngeal carcinoma-derived extracellular vesicles might serve as biomarkers for early diagnosis and therapeutic targets. The present review provides information on the biological and clinical significance of extracellular vesicles in tumors, especially in nasopharyngeal carcinoma ...
LRP-mediated agLDL uptake has proved to be one of the main mechanisms for intracellular lipid accumulation in human VSMCs,17-20 cells that have the capacity to secrete TF MP.22,27 In the present study, we hypothesized that agLDLs were able to induce cellular TF expression and activity. We found that both nLDL and agLDL induced TF mRNA after 2 hours of LDL exposure to the same level, in agreement with previous studies showing that nLDL and oxidized LDL induce TF mRNA in different cell types,12-14 which is likely unrelated to the receptor involved in LDL uptake. The increase in mRNA transcription levels induced by nLDL or agLDL did not require new protein synthesis but required a functional transcriptional mechanism. The increase in mRNA led to a strong increase in membrane-associated cellular TF protein expression. With regard to TF activity, the effect of agLDL on TF PCA, in contrast to a shorter time effect of nLDL, was maintained for 24 hours. The ability of nLDL to induce TF expression but ...
Background: Body fluids contain cell-derived vesicles ranging from 30 nm to 1 μm in diameter. The function, origin, and composition of these vesicles is disease dependent and therefore vesicles contain clinical information. The most common method to detect vesicles is flow cytometry, which guides vesicles through a laser beam in a hydrodynamically focused fluid stream. The unknown relationship between the measured light scattering intensity and the vesicle diameter resulted in unexplained contradictions between expected and observed results.. Methods: We combined light scattering measurements of polystyrene and silica beads with Mie calculations using an estimated refractive index of vesicles.. Results: We established the relationship between measured light scattering and the diameter of vesicles. We show that common gating strategies based on beads select vesicles (and cells) ranging from 800 to 2400 nm in diameter. For our flow cytometer, the smallest detectable silica beads were 204 nm, ...
Scientists report that adult cells reprogrammed to become primitive stem cells, called induced pluripotent stem cells (iPSCs), make more extracellular vesicles than other kinds of adult stem cells commonly used for this purpose in research. Extracellular vesicles are naturally abundant in many types of cells, which use the cargo-containing spheres to communicate with other cells. They are about one one-hundredth the diameter of a cell and can carry anything from fats and proteins to nucleic acids. When a cell releases an extracellular vesicle, other cells nearby slurp up the tiny packet and its contents, making it an attractive target for packaging treatments for diseased cells that are deteriorating or aging prematurely. To package a potential treatment in an extracellular vesicle, scientists typically use a cell called a mesenchymal stem cell, which is found among fat or bone marrow cells and gives rise to other fat and bone cells. Scientists genetically modify the stem cell to produce ...
This Research Topic addresses the emerging role of extracellular vesicles in cellular communications and immune responses. We will emphasize their use as biomarkers (research tool to better understand extracellular vesicle functions) and immune modulators (therapeutic tool to suppress or enhance immune responses). Extracellular Vesicles (EVs) are released in response to signals activating their parental cells. They are divided in two categories: small EVs (e.g. exosomes) and large EVs (e.g. microvesicles/ectosomes/microparticles). Small and large EVs differ in terms of size, release and budding mechanism, lipid and protein composition, and surface antigens. Research findings from the last decade suggest that EVs have an important, yet not completely understood, role in cell-cell communication. They can influence neighboring cells, act in an autocrine or paracrine manner, affect tumor growth, de novo-angiogenesis, and immunological functions. EVs are a novel and important tool to impact cell function and
STUDY QUESTION Are circulating microparticles (MPs) altered in young women with polycystic ovary syndrome (PCOS)? SUMMARY ANSWER Women with PCOS have elevated concentrations of circulating platelet-derived MPs, which exhibit increased annexin V binding and altered microRNA (miR) profiles compared with healthy volunteers. WHAT IS KNOWN ALREADY Some studies have shown that cardiovascular risk is increased in young women with PCOS but the mechanisms by which this occurs are uncertain. Circulating MPs are elevated in patients with cardiovascular disease but the characteristics of MPs in patients with PCOS are unclear. STUDY DESIGN, SIZE, DURATION Case-control study comprising 17 women with PCOS (mean ± SD; age 31 ± 7 years, BMI 29 ± 6 kg/m2) and 18 healthy volunteers (age 31 ± 6 years, BMI 30 ± 6 kg/m2). PARTICIPANTS/MATERIALS, SETTING, METHODS The study was conducted in a University hospital. Nanoparticle tracking analysis (NTA) and flow cytometry (CD41 platelet, CD11b monocyte, CD144 ...