Free-living cells of the fast-growing cowpea Rhizobium NGR234 were able to grow on a variety of carbon substrates at growth rates varying from 2.5 h on glucose or fumarate to 15.6 h on p-hydroxybenzoate. Free-living cells constitutively oxidized glucose, glutamate and aspartate but were inducible for all the other systems investigated. Bacteroids from root nodules of snake bean, however, were only capable of oxidizing C4-dicarboxylic acids and failed to oxidize any other carbon sources. Free-living cells of NGR234 possess inducible fructose and succinate uptake systems. These substrates are accumulated by active processes since accumulation is in hi bi ted by azide, 2,4-dini trop hen01 and carbonyl cyanide m-chlorophenyl hydrazone. Bacteroids failed to take up fructose although they actively accumulated succinate, suggesting that the latter substrate is significant in the development of an effective symbiosis.. ...
An Escherichia coli chromosomal DNA fragment cloned on a multicopy plasmid conferred resistance to carbonylcyanide m-chlorophenylhydrazone, nalidixic acid, and a number of other toxic compounds. The sequence of the cloned emr locus located at minute 57.5 of the chromosome revealed two open reading frames, emrA and emrB. emrB encodes a highly hydrophobic 56.2-kDa peptide, with 14 potential alpha-helices to span the inner membrane. The peptide is homologous to QacA, a multidrug-resistant pump from Staphylococcus aureus, and belongs to a gene family that includes tetracycline-resistant pumps of Gram-positive bacteria and the galactose/H+ symporter of E. coli. emrA encodes a putative 42.7-kDa peptide containing a single hydrophobic domain and a large C-terminal hydrophilic domain. An active pho-fusion to the C domain suggested that EmrA is a membrane protein. Disruption of emrB significantly increased sensitivity of cells to uncouplers. The cellular content of uncoupler increased in the order: ...
Solid State Proton Conductors von Philippe Knauth, Maria Luisa Di Vona und Buchbewertungen gibt es auf ReadRate.com. Bücher können hier direkt online erworben werden.
An uncoupler is a protonophore (CCCP, FCCP, DNP) which cycles across the inner mt-membrane with transport of protons and dissipation of the electrochemical proton gradient. Mild uncoupling may be induced at low uncoupler concentrations, the noncoupled state of ET capacity is obtained at optimum uncoupler concentration for maximum flux, whereas at higher concentrations an uncoupler-induced inhibition is observed. Abbreviation: U Reference: Noncoupled respiration ...
Principal Investigator:YAMANAKA Shinsuke, Project Period (FY):1993 - 1994, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Nuclear fusion studies
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BAM15, ST056388, mitochondrial protonophore uncoupler that does not depolarize the plasma membrane IDNUMBER ST056388 Other identifiers: SBB001916, ST4027337, T4021910, TBB011486, T3149443, ST236891 Formula: C16H10F2N6O MW: 340. 29 NAME: (2-fluorophenyl)amine SMILES: c12c(nc(c(n1)Nc1c(F)cccc1)Nc1c(F)cccc1)non2 InChI=1S/C16H10F2N6O/c17-9-5-1-3-7-11(9)19-13-14(20-12-8-4-2-6-10(12)18)22-16-15(21-13)23-25-24-16/h1-8H,(H,19,21,23)(H,20,22,24) InChIKey=OEGJBRZAJRPPHL-UHFFFAOYSA-N MDL: MFCD00373912 Contact us to order....
PINK1 can act as a molecular sensor of damaged mitochondria (Geisler et al., 2010a; Matsuda et al., 2010; Narendra et al., 2010b; Vives-Bauza et al., 2010). When mitochondria are experimentally depolarized, to mimic damage, with the use of uncouplers such as carbonyl cyanide m-chlorophenylhydrazone (CCCP) - which severely reduces the membrane potential across the IMM, which is required for TIM-mediated mitochondrial import of proteins - PINK1 no longer becomes processed by either MPP or PARL and the full-length 64-kDa form rapidly accumulates, spanning the OMM with its kinase domain facing the cytosol (Jin et al., 2010; Narendra et al., 2010b) (Poster Panel 2, lower part). This rapid accumulation of full-length PINK1 is blocked when the cells are pretreated with the protein synthesis inhibitor cycloheximide, indicating that the accumulated full-length PINK1 is a newly translated protein and its turnover is remarkably fast (Matsuda et al., 2010; Narendra et al., 2010b). In addition, when CCCP is ...
Rastegar Lari, Abdolaziz and Ardebili, Abdollah and Talebi, Malihe and Azimi, Leila (2014) Effect of Efflux Pump Inhibitor Carbonyl Cyanide 3-Chlorophenylhydrazone on the Minimum Inhibitory Concentration of Ciprofloxacin in Acinetobacter baumannii Clinical Isolates. Jundishapur Journal of Microbiology, 7 (1). pp. 1-5. ISSN 2008-4161 ...
We have used digital fluorescence imaging techniques to explore the interplay between mitochondrial Ca2+ uptake and physiological Ca2+ signaling in rat cortical astrocytes. A rise in cytosolic Ca2+ ([Ca2+]cyt), resulting from mobilization of ER Ca2+ stores was followed by a rise in mitochondrial Ca2+ ([Ca2+]m, monitored using rhod-2). Whereas [Ca2+]cyt recovered within ~1 min, the time to recovery for [Ca2+]m was ~30 min. Dissipating the mitochondrial membrane potential ( Dcm, using the mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxy-phenylhydrazone [FCCP] with oligomycin) prevented mitochondrial Ca2+ uptake and slowed the rate of decay of [Ca2+]cyt transients, suggesting that mitochondrial Ca2+ uptake plays a significant role in the clearance of physiological [Ca2+]cyt loads in astrocytes. Ca2+ signals in these cells initiated either by receptor-mediated ER Ca2+ release or mechanical stimulation often consisted of propagating waves (measured using fluo-3). In response to either ...
Looking for online definition of Proton-motive force in the Medical Dictionary? Proton-motive force explanation free. What is Proton-motive force? Meaning of Proton-motive force medical term. What does Proton-motive force mean?
The plasmoelectric effect: optically induced electrochemical potentials in resonant metallic structures Matthew T. Sheldon and Harry A. Atwater Thomas J. Watson Laboratories of Applied Physics, California
Recent Developments in Advanced Materials and Processes: Evaluation of Cytotoxic Activity of d-Metal Complexes with Hydrazone Ligands
MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, delta subunit (TIGR01145; EC 3.6.3.14; HMM-score: 118.5) ...
Electrophysiological and microfluorometric measurements were combined to analyse the responses of rat striatal medium spiny (MS) and large aspiny (LA) interneurons to the mitochondrial uncoupler carbonyl cyanide p-trifluoromethoxyphenylidrazone (FCCP). FCCP produced a membrane depolarisation coupled to an irreversible increase in intracellular calcium [Ca2+]i in MS. Conversely, LA interneurons hyperpolarised and a moderate [Ca2+]i rise was observed. Cyclosporin A, inhibitor of the mitochondrial membrane transition pore, prevented the FCCP-induced changes in LA interneurons, whereas only a partial reduction was observed in MS cells. The present results indicate that mitochondrial Ca2+ released into the cytosol may contribute to the selective vulnerability to metabolic impairment in striatal neuronal subtypes.. ...
These surface pores are attached to the electrosensory cells by canals filled with clear, viscous jelly, which let the fish detect weak electrical fields emitted by other animals in the sea - even signals as subtle as 5 nanovolts per centimetre.. As the scientists do not yet completely understand the way the jelly plays a part in this purpose, it is obvious that it somehow plays a particular role, because of its enormous convenience of conductivity, that the group claims to be the greatest previously documented to get a biological material.. At roughly 2 ± 1 millisiemens per centimetre, the conductivity of the jelly is just 40 times lower than the polymer Nafion, a synthetic material known for its superior conductivity.. Proton conductivity is just a type of electrical conductivity where hydrogen carry the charge ions, as opposed to electrons. Although electrons carry the charge in extremely conductive metals for example copper and silver, proton conductivity is definitely an essential process ...
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TY - JOUR. T1 - The level of stromal ATP regulates translation of the D1 protein in isolated chloroplasts. AU - Kuroda, Hiroshi. AU - Inagaki, Noritoshi. AU - Satoh, Kimiyuki. PY - 1992/1/1. Y1 - 1992/1/1. N2 - The synthesis of the D1 subunit of the reaction center of photosystem II is light-dependent in isolated chloroplasts. The mechanism of the regulation by light was analyzed using spinach chloroplasts. The light-regulated synthesis of the D1 protein was prevented by the addition of atrazine and the dependence on the concentration of atrazine of the inhibition was practically identical with that of the inhibition of photosynthetic electron transport in photosystem II, as measured by the photoreduction of 2,6-dichlorophenol indophenol. Inhibitors of photosynthetic phosphorylation, such as phloridzin, nigericin and carbonyl cyanide m-chlorophenylhydrazone, also inhibited the light-dependent synthesis of the D1 protein. Determination of the levels of ATP in chloroplasts and the rates of ...
The strategy applied in the structure refinement of accurate single-crystal neutron diffraction data for pseudo-centrosymmetric proton conducting systems can often be decisive. The application of a combination of judicious symmetry constraints and symmet. ...
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Proposes the law of motive force as a fundamental law of nature and establishes its relation to other laws and principles in thermodynamics With the aid
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The Section for Proton Conductors focuses on materials science and electrochemical systems and components based on proton conducting cells in a broad sense, at low to intermediate temperature (below 400 °C). The electrolytes include direct proton conductors as well as indirect proton conductors like aqueous hydroxide. Research also encompasses electrocatalysis and development of complete cells around proton conducting electrolytes.. We synthesize polymers and manufacture and characterize proton conducting membranes. Special attention is devoted to high temperature membranes (polybenzimidazole, PBI) with chemical stability in acid as well as in base. Such polymers have been successfully applied in high-temperature PEM fuel cells, a research field in which we are pioneers. Lately, similar membranes have been successfully applied in alkaline electrolysis cells. Solid or liquid inorganic proton conductors are also studied for electrolysis above 200 °C. Within electrocatalysis our research focus is ...
You are viewing an interactive 3D depiction of the molecule 1-[(4s)-4-{[(benzyloxy)carbonyl]amino}-4-carboxybutanoyl]-2-indolinecarboxylic acid (C22H22N2O7) from the PQR.
MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, epsilon subunit (TIGR01216; EC 3.6.3.14; HMM-score: 120.2) ...
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H+/e- stoichiometry for NADH dehydrogenase I and dimethyl sulfoxide reductase in anaerobically grown Escherichia coli cells.: Anaerobically grown Escherichia co
Vascular superoxide levels were also measured after incubation with NG and HG using a chemiluminescent probe (L-012) in the absence and presence of 1 and 2 above.. Results: ACh-induced vasodilation was impaired by HG vs. NG control (P,0.001). This impairment was modestly reversed by gp91 ds-tat (P, 0.001), but endothelial function was completely restored to the level observed under euglycemic conditions by CCCP (P=0.008 vs. HG, P,0.90 vs. NG). Endothelium-dependent vasodilation in HG vessels was greater after CCCP compared to gp91ds-tat (P,0.001). L-NAME inhibited all dilation to ACh indicating endothelium-dependence (data not shown). HG induced an increase in superoxide relative to NG (P,0.001), which was similarly suppressed by gp91ds-tat (P = 0.001) and CCCP (P=0.002).. Conclusion: HG-induced endothelial dysfunction is reversed with partial uncoupling of mitochondrial oxidative phosphorylation. Mitochondrial uncoupling more than NADPH oxidase blockade improves endothelial dysfunction due to ...
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Abstract A simple and rapid technique for the determination of the d -amino acids which are oxidized by d -amino acid oxidase has… Expand ...
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The physiological roles of polyphosphates (polyP) recently found in arthropod mitochondria remain obscure. Here, the relationship between the mitochondrial membrane exopolyphosphatase (PPX) and the energy metabolism of hard tick Rhipicephalus microplus embryos are investigated. Mitochondrial respiration was activated by adenosine diphosphate using polyP as the only source of inorganic phosphate (Pi) and this activation was much greater using polyP3 than polyP15. After mitochondrial subfractionation, most of the PPX activity was recovered in the membrane fraction and its kinetic analysis revealed that the affinity for polyP3 was 10 times stronger than that for polyP15. Membrane PPX activity was also increased in the presence of the respiratory substrate pyruvic acid and after addition of the protonophore carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. Furthermore, these stimulatory effects disappeared upon addition of the cytochrome oxidase inhibitor potassium cyanide and the activity was completely
Proton motive force, a necessity for all living cells, is generated and exploited by asignificant number of membrane-bound enzymes and transporters. The focus of thisthesis was to understand the physiological relevance, structure and mechanism of threesuch bacterial enzymes: proteorhodopsin; transhydrogenase; and AcrB. Proteorhodopsin, common in surface living bacteria, is demonstrated to exist also inphylum Bacteriodetes, the third most abundant class of ocean bacteria. Evidence is alsopresented that its presence stimulates growth of one such bacterium in the presence oflight. This finding indicates that proteorhodopsin associated growth enhancementcould contribute to the primary production in earths biosphere. Furthermore,optimisation of proteorhodopsin synthesis in vitro and from E. coli shows that yieldssufficient for structural studies by NMR and X-ray crystallography are achievable. Over-production of functional transhydrogenase with an altered sub-unit composition,where the β-subunit is split
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PINK1-YFP R98F (green) is found localized to mitochondria (red) in the presence of the mitochondrial depolarizing agent CCCP (carbonyl cyanide-m-chlor...
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Structure and function of SemiSWEET.(a) Time course of [14C]-sucrose uptake by proteoliposomes containing EcSemiSWEET (solid black squares) or empty control lip
The International Cyanide Management Institute (ICMI) announced today that Victor Masson Transportes Cruz del Sur S.A. (Cruz del Sur), a cyanide transporter in Argentina, has been recertified in full compliance with the International Cyanide Management Code (Cyanide Code).
P62-mediated mitophagy inducer is a mitophagy regulator which activates mitophagy without recruiting Parkin or collapsing ΔΨm and retains activity in cells devoid of a fully functional PINK1/Parkin pathway. - Mechanism of Action & Protocol.
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