TY - JOUR. T1 - Significant suppression of aldolase B, carbonic anhydrase III and alcohol dehydrogenase in liver cytosol of rats treated with a highly toxic coplanar PCB. AU - Kato, H.. AU - Ishii, Y.. AU - Hatsumura, M.. AU - Ishida, T.. AU - Nakayama, I.. AU - Ariyoshi, N.. AU - Oguri, K.. N1 - Copyright: Copyright 2018 Elsevier B.V., All rights reserved.. PY - 1997. Y1 - 1997. UR - http://www.scopus.com/inward/record.url?scp=0030893411&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0030893411&partnerID=8YFLogxK. U2 - 10.1248/jhs1956.43.P20. DO - 10.1248/jhs1956.43.P20. M3 - Article. AN - SCOPUS:0030893411. VL - 43. SP - P20. JO - Japanese Journal of Toxicology and Environmental Health. JF - Japanese Journal of Toxicology and Environmental Health. SN - 0013-273X. IS - 1. ER - ...

Mouse Monoclonal Anti-Carbonic Anhydrase III/CA3 Antibody (4A12-1A3). Validated: WB, ELISA, IHC-P. Tested Reactivity: Human. 100% Guaranteed.

Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects around 1 % of the human population worldwide. RA diagnosis can be difficult as there is no definitive test for its detection. Therefore, the aim of this study was to identify biomarkers that could be used for RA diagnosis. Sera from a collagen-induced arthritis mouse model were used to select potential biomarkers for RA diagnosis by phage display technology. In silico and in vitro analyses were performed to characterize and validate the selected peptides. Samples were classified into three groups: RA; two other immune-mediated rheumatic diseases (systemic lupus erythematosus (SLE) and ankylosing spondylitis (AS)); and healthy controls (HC). Enzyme-linked immunosorbent assay (ELISA) was carried out to determine antibody levels, and diagnostic parameters were determined by constructing receiver operating characteristic curves. Mass spectrometry and Western blot were performed to identify the putative autoantigen that was

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Human carbonic anhydrase 8 (CA-VIII) is an acatalytic isoform of the α -CA family. Though the protein cannot hydrate CO2, CA-VIII is essential for calcium (Ca2+) homeostasis within the body, and achieves this by allosterically inhibiting the binding of inositol 1,4,5-triphosphate (IP3) to the IP3 receptor type 1 (ITPR1) protein. However, the mechanism of interaction of CA-VIII to ITPR1 is not well understood. In addition, functional defects to CA-VIII due to non-synonymous single nucleotide polymorphisms (nsSNVs) result in Ca2+ dysregulation and the development of the phenotypes such as cerebellar ataxia, mental retardation and disequilibrium syndrome 3 (CAMRQ3). The pathogenesis of CAMRQ3 is also not well understood. The structure and function of CA-VIII was characterised, and pathogenesis of CAMRQ3 investigated. Structural and functional characterisation of CA-VIII was conducted through SiteMap and CPORT to identify potential binding site residues. The effects of four pathogenic nsSNVs,

Measuring proteins involved in glucose and fatty acid metabolism before and after an 8-wk VLED reflects the metabolic adaptations occurring in adipose tissue linked to energy expenditure. More specifically, the decrease of AldoC, an enzyme of glycolysis, is correlated with the decrease in AEE, and the non-significant change HADHsc, a crucial enzyme for mitochondrial beta-oxidation, is negatively correlated with the adaptation in REE. Furthermore, there is a correlation between the increase in FABP4, the intracellular fatty acid transporter, and the decrease in fat mass, and a correlation between the decrease in AldoC and the decrease in fat free mass. FABP4, AldoC and HADHsc are all positively correlated.. The increased FABP4 after the VLED weight loss is in accordance with previous results in obese subjects [11, 12, 44]. It is in line with an elevation in intracellular trafficking of fatty acids, which is expected during a negative energy balance when lipolysis is stimulated, with the release ...

Supplementary MaterialsS1 Dataset: Minimal data established. clinic. The blood S-glutathionylated GAPDH levels in the AD patients were significantly (p 0.001) higher (752.7 301.7 ng/dL) than those in the controls (59.92 122.4 ng/dL), irrespective of gender and age. For AD analysis, the criterion blood S-glutathionylated GAPDH level 251.62 ng/dL exhibited 95.74% sensitivity and 92.67% specificity. In fact, the individuals aged 70C89 years, namely, 37 patients from your psychosomatic medical center and 42 healthy individuals, showed significant blood S-glutathionylated GAPDH levels (230.5 79.3 and 8.05 20.51 ng/dL, respectively). This getting might indicate neurodegenerative AD progression in psychosomatic individuals and suggests that the degree of neuronal apoptosis during AD progression might be sensitively evaluated based on the level of S-glutathionylated GAPDH in blood. Introduction The majority of dementia cases can be diagnosed as AD [1], which is definitely classified like a ...

Carbonic anhydrase III (CAIII) is a member of a multigene family (at least six separate genes are known) that encodes carbonic anhydrase isozymes. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The expression of the CA3 gene is strictly tissue specific and present at high levels in skeletal muscle and much lower levels in cardiac and smooth muscle. A proportion of carriers of Duchenne muscle dystrophy have a higher CA3 level than normal. The gene spans 10.3 kb and contains seven exons and six introns. [provided by RefSeq, Oct 2008 ...

How many net carbs are in Tomato Paste? Amount of Proline in Tomato paste: How much Serine is in Tomato paste? thank you! Amount of Tyrosine in Tomato paste: How much Valine is in Tomato paste? Tomato Paste #1 All Concentrations in ppm Chloride Sodium Potassium Magnesium Calcium Dilution Replicate 1 5210 2010 12,220 420 130* 166.32 Replicate 2 5270 200 12,320 430 130* 166.32 Replicate 3 5270 2070 12,340 430 140* 166.32 Average 5250 2050 12,290 430 130* RSD (%) 0.68 1.6 0.55 1.4 2.3 *Below reporting limit of 166 ppm . Amount of Choline in Tomato paste: How much Betaine is in Tomato paste? It contains mostly carbohydrates -- about 3 grams in 1 tablespoon -- along with a tiny amount of protein and almost no fat. Amount of Zinc in Tomato paste: How much Copper is in Tomato paste? Nutrition facts and Information for Hunts Tomato Paste, canned in Home ... Vitamin C and Potassium. Caloric Ratio Pyramid. Having kidney disease can make it harder for your body to maintain a healthy potassium level of ...

C. Biswas, K. Devarajan, T. K. Panda, and S. S. K. Raavi, Enhanced Broadband Emission in Novel Phenanthroimidazole Derivative Molecules via Excited State Intramolecular Proton Transfer, in OSA Advanced Photonics Congress (AP) 2020 (IPR, NP, NOMA, Networks, PVLED, PSC, SPPCom, SOF), L. Caspani, A. Tauke-Pedretti, F. Leo, and B. Yang, eds., OSA Technical Digest (Optical Society of America, 2020), paper JTh3G.5 ...

I love this stuff. The other day I was making a soup out of some kale from the garden, some leftover chickpeas and a few odd potatoes (along with a bay leaf, some garlic and fresh thyme). But it needed something.. Found in the fridge: an almost used-up tube of Mutti tomato paste. I squeezed in a little and my soup came together. I really wouldnt do it with any other tomato paste, but Mutti of Parma, Italy, produces the best.. Theyve been making it since 1899. Their paste is double-concentrated and made only from tomatoes and salt. Thats it. After all the companys motto is solo pomodoro. Per passione - only tomatoes, for passion.. The firm uses only Italian tomatoes and a slow process of dehydration. Its easy to taste the difference - none of that sludgy, bitter or cooked-down taste of regular tomato paste. This tastes bright and carries the deep flavor of sun-warmed tomatoes.. Use it to beef up the tomato taste of a sauce, or add an underlying tomato note to a brasato or stew.. Any cook ...

El Rashidi El Mizan tomato paste is made from fresh ripe tomatoes. Add El Rashidi El Mizan tomato paste to your favorite recipes and dishes to enrich the flavors of your sauces, pasta, or pizza. El Rashidi El Mizan carefully fresh tomatoes guarantee the taste and quality of the tomato paste that enhances the cooking experience.. ...

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...

Close The Infona portal uses cookies, i.e. strings of text saved by a browser on the users device. The portal can access those files and use them to remember the users data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser. ...

Centos all-natural, authentically prepared tomato paste is 100% Italian and a favorite of ours for years. We love the innovative packaging that makes using and sto...

Chances are the recipe youre making calls for only a fraction of that can of tomato paste. Here are five genius ways to put the rest to good use.

Turkey exported tomato paste to 99 countries during the first eight months of this year, according to an exporter associations data.

1E9O: Conformational Variability of the Cu Site in One Subunit of Bovine Cuzn Superoxide Dismutase: The Importance of Mobility in the Glu119-Leu142 Loop Region for Catalytic Function

UV and IR photochemistries of malonaldehyde, the simplest molecule exhibiting an intramolecular proton exchange, have been studied in four cryogenic matrices at 4.3 K, N2, Ne, Ar and Xe. Samples have been irradiated using a UV and IR OPO type tunable laser, and with a broad band UV mercury lamp. UV and IR spectra have been recorded and compared with theoretical calculations carried out at the SAC-CI/6-31++G(d,p) (UV transitions) and B3LYP/6-311++G(2d,2p) (IR spectra) levels of theory. After de- position, the intramolecularly H-bonded form is found exclusively, while several open forms are formed upon UV irradiation ...

Canned goods hidden away behind the pantry door, waiting patiently to be put to use. Will it be the canned corn tonight? Perhaps youll use the tomato paste or chipotles in adobo sauce? Or go for some chicken noodle soup? Canned goods inform many of our meals. Good quality tools can make prepping those meals a cinch. A good corkscrew can make getting into your next bottle of vino that much sweeter. A microplane will zip you through zesting, and sharp knives make chopping, slicing, and dicing a breeze. ...

To make tomato paste, start by sautéing chopped tomatoes in a skillet with some olive oil for 10 minutes. Pizza and marinara sauce are similar, but not the same. The sauce will cover 4-6 large pizza bases. Let it simmer and stir it constantly until it is reduced by half. Chances are that it will taste a bit different now that youve added liquids, so be prepared to add more salt or pepper. Bring the tomatoes to a boil over high heat. Heres a recipe for how to slow roast tomatoes. It creates an incredibly rich tomato flavor. Out of these cookies, the cookies that are categorized as necessary are stored on your browser as they are essential for the working of basic functionalities of the website. Support Organic Authority by subscribing to Premium and view the site with no ads. Tomato paste creates a thicker sauce which prevents pizza from getting soggy. Ingredients 1/4 cups + 2 tablespoons organic tomato paste 2 tablespoons extra virgin olive oil 2 teaspoons dried oregano 1 teaspoon garlic ...

Semantic Scholar extracted view of Follow-up studies of iliofemoral arterial reconstruction in arteriosclerosis obliterans. by Sarah Hoye et al.

See all results for Spaghetti Casserole No Null No (tomato Paste) Recipes. Find more recipes at Foodily.com - the largest recipe search site - and see the ones your friends like.

In addition to the Steered MD (SMD) approach described starting on page 108 of the manual, a new set of Adaptively Biased MD and related methods has been introduced for Amber10 (See page 121 and also be aware that this tutorial uses an Amber11 addition, LCOD, to these methods. Adding LCOD capabilities to Amber10 is described below). The whole Adaptively Biased MD section (4.6) should be studied for background to this tutorial. The new implementation of the SMD method specifically is described starting on page 125. The tutorial is a brief test case using steered MD to force an intramolecular proton transfer (PT) in malonaldehyde (pictured). The simulation was carried out in vacuum with the QMMM DFTB Hamiltonian using the LCOD (linear combination of distances) reaction coordinate. The QM region consisted of the whole molecule. These results should be compared with the Empirical Valence Bond (EVB) section, specifically to Figure 3.4 (page 77, closed triangles). As indicated above the LCOD reaction ...

1TG3: Structural and Kinetic Characterization of Active-Site Histidine as a Proton Shuttle in Catalysis by Human Carbonic Anhydrase II

为解决新疆番茄酱加工废水排放量大、处理效果参差不齐的情况，在SBR（序批式反应器）中接种絮体污泥，以人工合成番茄酱加工废水为基质成功培养出粒径为0.50~1.61 mm的好氧颗粒污泥，并采用扫描电镜、三维荧光光谱（3D EEM）、激光共聚焦（CLSM）、死活细菌染色以及高通量测序等技术表征活性污泥的颗粒化过程.结果表明，颗粒污泥沉降性能良好，CODCr、NH4+-N、PO43--P的平均去除率分别为90%、85%、45%.扫描电镜下，椭球状的颗粒污泥轮廓清晰，结构密实.蛋白质在颗粒化过程中逐渐增加，α-多糖、β-多糖和蛋白质贯穿整个颗粒截面，在颗粒中分布广泛，构成了颗粒的骨架.颗粒内部的孔隙为溶解氧和营养物质传递提供了条件，因此活细菌则更多地靠近颗粒边缘及内部的孔隙周围，并包裹着死细菌.颗粒化过程中

Most loss-of-function diseases are caused by aberrant folding of important proteins. These proteins often misfold due to inherited mutations. The rare disease marble brain disease (MBD) also known as carbonic anhydrase II deficiency syndrome (CADS) can manifest in carriers of point mutations in the human carbonic anhydrase II (HCA II) gene. We have over the past 10-15 years studied the folding, misfolding and aggregation of the enzyme human carbonic anhydrase II. In summary our HCA II folding studies have shown that the protein folds via an intermediate of molten-globule type, which lacks enzyme activity and the molten globule state of HCA II is prone to aggregation. One mutation associated with MBD entails the His107Tyr (H107Y) substitution. We have demonstrated that the H107Y mutation is a remarkably destabilizing mutation influencing the folding behavior of HCA II. A mutational survey of position H107 and a neighboring conserved position E117 has been performed entailing the mutants H107A, ...

URUMQI, Sept. 15 (Xinhua) -- At a packaging workshop of canned tomato paste in Yanqi Hui Autonomous County, northwest Chinas Xinjiang Uygur Autonomous Region, Aysam Sawut watches over a control console while listening to music on her headphones.. The 29-year-old skilled operator and 26 other workers are employed at the highly automated factory which produces around 6,000 tonnes of tomato paste annually. Half of its products are sold overseas.. Aysam Sawuts factory is certainly relevant to pizza and crisp lovers. One in five canned tomato paste available on the global market is likely to have been produced in Xinjiang, one of the worlds largest producers and exporters of the sweet-sour product.. In 2020 alone, the region produced nearly 720,000 tonnes of tomato products, accounting for more than 85 percent of Chinas total.. Yang Ruisheng deems autumn the most joyful season of the year. A tomato farmer and head of a local rural cooperative in Yanqi, Yang owns about 40 hectares of tomato ...

View reliable Canned Food manufacturers on Made-in-China.com. This category presents Tomato Paste, Canned Tomato Paste, from China Canned Food suppliers to global buyers., page 3

Aryl sulfonamides are a widely used drug class for the inhibition of carbonic anhydrases. In the context of our program of photochromic pharmacophores we were interested in the exploration of azobenzene-containing sulfonamides to block the catalytic activity of human carbonic anhydrase II (hCAII). Herein, we report the synthesis and in vitro evaluation of a small library of nine photochromic sulfonamides towards hCAII. All molecules are azobenzene-4-sulfonamides, which are substituted by different functional groups in the 4 -position and were characterized by X-ray crystallography. We aimed to investigate the influence of electrondonating or electron-withdrawing substituents on the inhibitory constant Ki. With the aid of an hCAII crystal structure bound to one of the synthesized azobenzenes, we found that the electronic structure does not strongly affect inhibition. Taken together, all compounds are strong blockers of hCAII with K-i = 25-65 nM that are potentially photochromic and thus combine ...

Entrepreneurs say the crisis has been worsened by the central banks decision to keep an artificially high exchange rate, which has dried up dollar supplies, forcing firms to buy them on the black market at a 40 per cent premium.Where the foreign exchange is not available, we are cutting down our operations. For example, we had a tomato-based processing plant, we have shut it down, Tomato paste is a staple food in Nigeria but the country imports much of its supplies from China. Dangotes plant opened only last year amid much talk from officials predicting a new era of Nigeria producing its own tomato paste, displacing costly imports ...

ESCUDERO-ALMANZA, Dalila Jacqueline et al. Carbonic Anhydrase and Zinc in Plant Physiology. Chilean J. Agric. Res. [online]. 2012, vol.72, n.1, pp.140-146. ISSN 0718-5839. http://dx.doi.org/10.4067/S0718-58392012000100022.. Carbonic anhydrase (CA) (EC: 2.4.1.1) catalyzes the rapid conversion of carbon dioxide plus water into a proton and the bicarbonate ion (HCO3-) that can be found in prokaryotes and higher organisms; it is represented by four different families. Carbonic anhydrase is a metalloenzyme that requires Zn as a cofactor and is involved in diverse biological processes including pH regulation, CO2 transfer, ionic exchange, respiration, CO2 photosynthetic fixation, and stomatal closure. Therefore, the review includes relevant aspects about CA morphology, oligomerization, and structural differences in the active site. On the other hand, we consider the general characteristics of Zn, its geometry, reactions, and physiology. We then consider the CA catalysis mechanism that is carried out ...

MediSavvy has been developed as an educational platform and learning resource for health professionals, as well as patients that are keen to learn more about their conditions. ...

Nutrition Facts for Chestnut Hill - Tomato Paste - Get a bar chart of the top 10 nutrients, and click to see an expanded list of over 151 nutrients, including amino acids.

5315 K-ras is mutated in many lung adenocarcinomas, but the nature of its contribution to the development and maintenance of these cancers is poorly understood. Generation of reactive oxygen species (ROS) is proposed to be part of its mechanism of action. We pursued this hypothesis in 12 lung adenocarcinoma cell lines expressing mutant K-ras and 8 lines with only wildtype K-ras. K-ras total protein showed a 14-fold variation in amount among the cell lines and was as high on average in cells with wildtype K-ras, as in those with mutant protein. As expected K-ras activity was much higher in lines with mutant K-ras, varied 6-fold among these, and correlated strongly with protein levels. Mn and CuZn superoxide dismutase (SOD) protein levels varied approximately 2-fold among the cell lines. Only MnSOD varied significantly with the parameters measured, and showed a weak positive correlation with levels of superoxide (as measured by reduction of nitroblue tetrazolium) and a strong negative correlation ...

Shop a large selection of Sample Prep Tools for Mass Spectrometry products and learn more about Alfa Aesar Carbonic Anhydrase Digest Standard, Certified Mass Spec Grade Quantity:

SWISS-MODEL Template Library (SMTL) entry for 6wdl.1. Cryo-EM of elongating ribosome with EF-Tu*GTP elucidates tRNA proofreading (Non-cognate Structure V-B1)

Abstract Tomato pomace is a by-product of producing tomato paste and sauce composed by a mixture of seed, skin, and pulp that could be used in animal diets. It ...

Protein S-glutathionylation is a reversible post-translational modification regulating sulfhydryl homeostasis. However, little is known about the proteins and pathways regulated by S-glutathionylation in whole organisms and current approaches lack the sensitivity to examine this modification under basal conditions. We now report the quantification and identification of S-glutathionylated proteins from animal tissue, using a highly sensitive methodology combining high-accuracy proteomics with Tandem Mass Tagging to provide precise, extensive coverage of S-glutathionylated targets in mouse liver. Critically, we show significant enrichment of S-glutathionylated mitochondrial and Krebs cycle proteins, identifying that S-glutathionylation is heavily involved in energy metabolism processes in vivo . Furthermore, using mice nulled for glutathione S-transferase Pi (GSTP) we address the potential for S-glutathionylation to be mediated enzymatically. The data demonstrate the impact of S-glutathionylation ...

Arteriosclerosis obliterans is an occlusive arterial disease most prominently affecting the abdominal aorta and the small- and medium-sized arteries of the lower extremities, which may lead to absent dorsalis pedis, posterior tibial, and/or popliteal artery pulses. It is characterized by fibrosis of the tunica intima and calcification of the tunica media. Arteriosclerosis Monckebergs arteriosclerosis Skin lesion James, William D.; Berger, Timothy G.; et al. (2006). Andrews Diseases of the Skin: clinical Dermatology. Saunders Elsevier. ISBN 0-7216-2921-0 ...

In the catalysis of the hydration of carbon dioxide and dehydration of bicarbonate by human carbonic anhydrase II (HCA II), a histidine residue (His64) shuttles protons between the zinc-bound solvent molecule and the bulk solution. To evaluate the effect of the position of the shuttle histidine and …

SLC4A5 is a sodium-bicarbonate co-transporter involved with sodium homeostasis. Based on unpublished data, two SLC4A5 single nucleotide polymorphisms (SNPs rs1017783 and rs7571842) have been highly associated with an individuals salt-sensitivity status. Since the renal proximal tubule (RPT) regulates a large percentage of renal sodium transport, we investigated whether SLC4A5 was present in this nephron segment. Using confocal immunofluorescence microscopy, we found expression of SLC4A5 in human RPT cell plasma membrane and intracellular membrane vesicles. We then examined the physiologic implications of the SLC4A5 SNPs in human RPT cells. Using immunoblotting and RT-PCR, we found no significant differences in basal SLC4A5 expression in RPT cells between individuals that are homozygous variant at both SNPs and individuals that are wild-type (WT) for both alleles. Stimulation of the dopaminergic system with 1μM fenoldopam, or the renin-angiotensin system with 10 nM angiotensin II or 10 nM ...

The photophysics of the fluorescent probe Lucifer yellow CH has been investigated using fluorescence spectroscopic and computational techniques. The nonradiative rate is found to pass through a minimum in solvents of intermediate empirical polarity. This apparently anomalous behavior is rationalized by considering the possibility of predominance of different kinds of nonradiative processes, viz. intersystem crossing (ISC) and excited-state proton transfer (ESPT), in solvents of low and high empirical polarity, respectively. The feasibility of the proton transfer is examined by the structure determined by the density functional theory (DFT) calculations. The predicted energy levels based on the time-dependent density functional theory (TD-DFT) method in the gas phase identifies the energy gap between the S-1 and nearest triplet state to be close enough to facilitate ISC. Photophysical investigation in solvent mixtures and in deuterated solvents clearly indicates the predominance of the ...

cansSAR 3D Structure of 3DC3 | USE OF CARBONIC ANHYDRASE II, IX ACTIVE-SITE MIMIC, FOR THE PURPOSE OF SCREENING INHIBITORS FOR POSSIBLE ANTI-CANCER PROPERTIES | 3DC3_A | Carbonic anhydrase 2 - Also known as CAH2_HUMAN, CA2. Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Interacts with SLC26A6 isoform 4 (via C-terminus cytoplasmic domain).

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BioAssay record AID 644085 submitted by ChEMBL: Inhibition of human carbonic anhydrase 2 using 4-nitrophenylacetate as substrate after 3 mins using spectrophotometry by Lineweaver-Burk plot analysis.

In this study, bovine articular and human chondrocytes from the C-20/A4 cell line were tested for the functional activity and molecular presence of the enzyme carbonic anhydrase. This enzyme is classically considered to be important in the maintenance of high cellular buffering capacity by catalysing the slow attainment of equilibrium between CO(2) and HCO(3)(-). The first functional assay measured the rate of pH equilibration after administration of a fixed dose of CO(2) solution to cell lysates. Compared to positive controls (human erythrocytes, murine M1 cells and purified carbonic anhydrase), chondrocyte lysates attained equilibrium at a significantly slower rate, similar to the rate obtained with a negative control (Xenopus oocytes). A second functional assay studied CO(2) hydration kinetics in intact C-20/A4 cells, using a pH-sensitive fluorescent dye, as the CO(2) content of the extracellular solution was changed. It was shown that C-20/A4 cells accelerate hydration only to a small degree.

The enzyme carbonic anhydrase has been well characterized in mammalian systems, but the structural properties of the plant isozymes remain elusive. To investigate the nature of the zinc-binding site in spinach carbonic anhydrase, we targeted potential zinc ligands for mutagenesis and examined the re …

In the catalysis of the hydration of carbon dioxide and dehydration of bicarbonate by human carbonic anhydrase II (HCA II), a histidine residue (His64) shuttles protons between the zinc-bound solvent molecule and the bulk solution. To evaluate the effect of the position of the shuttle histidine and pH on proton shuttling, we have examined the catalysis and crystal structures of wild-type HCA II and two double mutants: H64A/N62H and H64A/N67H HCA II. His62 and His67 both have their side chains extending into the active-site cavity with distances from the zinc approximately equivalent to that of His64. Crystal structures were determined at pH 5.1-10.0, and the catalysis of the exchange of (18)O between CO(2) and water was assessed by mass spectrometry. Efficient proton shuttle exceeding a rate of 10(5) s(-)(1) was observed for histidine at positions 64 and 67; in contrast, relatively inefficient proton transfer at a rate near 10(3) s(-)(1) was observed for His62. The observation, in the crystal ...

If you have a question about this talk, please contact Nick Riddle.. Additional Talk: Host - Christof Schwiening. Abstract not available. This talk is part of the Foster Talks series.. ...

TY - JOUR. T1 - Absence of host NF-κB p50 induces murine glioblastoma tumor regression, increases survival, and decreases T-cell induction of tumor-associated macrophage M2 polarization. AU - Barberi, Theresa. AU - Martin, Allison. AU - Suresh, Rahul. AU - Barakat, David J.. AU - Harris-Bookman, Sarah. AU - Drake, Charles G.. AU - Lim, Michael. AU - Friedman, Alan David. PY - 2018/1/1. Y1 - 2018/1/1. N2 - High-grade gliomas harbor abundant myeloid cells that suppress anti-tumor immunity and support tumor growth. Targeting transcription factors, such as NF-κB p50, that mediate suppressive myeloid M2 polarization may prove therapeutic. GL261-Luc glioblastoma cells were inoculated into wild-type and p50−/− mice, followed by analysis of tumor growth, survival, tumor myeloid cells, and T cells. The absence of host p50 slows tumor growth and enables regression in 30% of recipients, leading to prolonged survival. Tumors developing in p50−/− mice possess a greater concentration of ...

Carbonic anhydrase IX (CAIX) is a hypoxia-induced, membrane-tethered enzyme that is highly expressed in many cancers. It catalyses the hydration of CO 2 to HCO3- and H+, and the reverse dehydration reaction. Recent studies have shown an important role for CAIX in pH regulation and it has been speculated that CAIX may play a role in supporting cancer progression towards more aggressive forms of the disease. Clinical correlative studies in many tumours have shown that high expression is related to poor outcome. In the present study, we have selected antigen-binding antibody fragments (Fab) against human CAIX by phage-display, and tested these for inhibitory potency on CAIX catalytic activity. Inhibition was assessed from the kinetics of the CAIX-catalysed reaction, using assays performed on intact cells over-expressing CAIX, and their CAIX-containing membrane fragments. Inhibition was also assessed in multi-cellular tissue-models (spheroids) from the kinetics of CO2 venting. We have identified a Fab

Recombinant Carbonic Anhydrase VII (CA7) Protein. Species: Rat (Rattus). Source: Escherichia coli (E. coli). Order product ABIN6302725.

The cytosolic isoform XIII is a recently discovered member of the human carbonic anhydrase (hCA, EC 4.2.1.1) family. It is selectively expressed among other tissues in the reproductive organs, where it may control pH and ion balance regulation, ensuring thus proper fertilization conditions. The authors report here the X-ray crystallographic structure of this isozyme in the unbound state and in complex with a classical sulfonamide inhibitor, namely acetazolamide. A detailed comparison of the obtained structural data with those already reported for other CA isozymes provides novel insights into the catalytic properties of the members of this protein family. On the basis of the inhibitory properties of acetazolamide against various cytosolic/transmembrane isoforms and the structural differences detected within the active site of the various CA isoforms, further prospects for the design of isozyme-specific CA inhibitors are here proposed. Proteins 2008. (c) 2008 Wiley-Liss, Inc. Crystal structure of ...

Rafael MS, Laizé V, Bensimon-Brito A, Leite RB, Schüle R, M. Cancela L. Four-and-a-half LIM domains protein 2 (FHL2) is associated with the development of craniofacial musculature in the teleost fish Sparus aurata. Cell Mol Life Sci. 2012;69(3):423-34. doi:10.1007/s00018-011-0754-y ...

Who knew that a Mao-era Chinese agricultural conglomerate dominates the African tomato market and supplies global food companies? A hundred (...)

SWISS-MODEL Template Library (SMTL) entry for 1t9n.1. Effect of Shuttle Location and pH Environment on H+ Transfer in Human Carbonic Anhydrase II

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Well you run and you run to catch up with the Sun but its sinking, racing around to come up behind you again. The Sun is the same in a relative way but youre older, shorter of breath and one day closer to death. -Pink Floyd For the last four-and-a-half billion years, the Earth has spun on its axis, orbiting its parent star: our Sun. Today, our home planet looks something like this.

Video: Jelle Bakker, who has a fondness for complex marble runs, made this epic 228m long sand track that descends some 38m from its start to its finish. The 12 marbles competing in this video take almost four-and-a-half minutes to complete the run, and youll be surprised to find yourself…. ...

This story was pencilled, inked and lettered in four-and-a-half hours, in front of a watching audience, at a charity work-in which took place at UK Comic Art Convention 1988 ...

Over the weekend, I climbed the Everest of lectin-free conversion dishes. I attempted a slow-cooked chili with no tomatoes, no peppers, and no beans. Ive tried Dr. Gundrys skinny chili in the past, and, although delicious, I was left wanting a chili that had a thick chili texture (i.e. one with tomato paste) In the…. ...

Execution:. A chili con carne with minced-meat recipe that is super easy and fast prepared.. Heat a large, deep pan, pour in the olive oil and sauté the minced meat until it is nicely crumbly.. Add the tomato paste, pour in the beef soup and let cook.. Add the corn, tomatoes, peppers and beans and simmer until the peppers are soft.. Before serving, season the chili con carne with the spices. Serve with nachos as a side dish!. Good luck!. ...

Take pleasure in contemporary-baked lasagna in lower than forty five minutes with our Lasagna Little Massive Meal. I let the lasagna sit at room temp about an hour before I baked it and then upped the cooking time by quarter-hour, including a few minutes under broil, to brown the cheese a bit. Primarily as a result of I like the style of the tomato paste, but additionally because the recipe my mother used known as for it. It helps thicken it up. I am going to let you understand how it turns out. Alternately, if fresh pasta is difficult to seek out the place you are, replenish on no-boil (entire wheat, if attainable) lasagna sheets.. Oh yeah, I also wanted to say that my lasagna was a tad soupy, and I imagine that it might have been worse had I not put it in the fridge in a single day (the place everyrhing had a chance to attract up among the juices. Individuals like it their manner, and will struggle to the demise for it. Im not going to sit down right here and lecture you on the price of ...

I just had an intense, sudden food craving. When I lived in Japan, I bought this amazing sauce/spread stuff. I have no idea what it was called (dont read Japanese), nor do I know what it was supposed to be used for. I used it on bread. It was a tomato paste/sauce with corn kernels in it. I…