OTHER GLYCAN DEGRADATION GlcNAc GlcNAc Fuc Neu5Ac Gal Asn α2 β1 β1 Man α1 GlcNAc Neu5Ac Gal α2 β1 β1 Man α1 β1 GlcNAc Man β1 β1 GlcNAc β1 α1 Fuc α1 Neu5Ac α2 Neu5Ac α2 Gal β1 Neu5Ac α2 Neu5Ac α2 GalNAc Neu5Ac α2 Neu5Ac α2 Neu5Ac α2 β1 Gal β1 Glc β1 ceramide Ganglioside N-glycan N-Glycan ...
According to National Geographic, the inner core of the Earth ranges between 9,000 and 13,000 degrees Fahrenheit. This is approximately as high as the temperature of the suns...
H. Casajus, S. Tranchimand, D. Wolbert, C. Nugier-Chauvin, S. Cammas-Marion, J. Appl. Polym. Sci., 2016, DOI: 10.1002/app.44604.. • A. Pennec, R. Daniellou, P. Loyer, C. Nugier-Chauvin, V. Ferrieres Carbohydr. Res. 2015, 402, 50-55.. • A. Pennec, L. Legentil, L. Herrera-Estrella, V. Ferrières, A.-L. Chauvin, C. Nugier-Chauvin, Green Chem., 2014, 16, 3803-3809.. • I. Chlubnová, B. Sylla, C. Nugier-Chauvin, R. Daniellou, L. Legentil, B. Králová, V. Ferrières Nat. Prod. Rep., 2011, 28, 937-952.. • I. Chlubnová, D. Filipp, V. Spiwok, H. Dvořáková, R. Daniellou, C. Nugier-Chauvin, B. Králová, V. Ferrières Org. Biomol. Chem., 2010, 8, 2092-2102.. Invited talks at Conferences: ...
The N-linked carbohydrate chains of recombinant human erythropoietin expressed in CHO cells were quantitatively released with peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase F, separated from the remaining O-glycoprotein by gel-permeation chromatography, and subsequently fractionated via FPLC on Mono Q, HPLC on Lichrosorb-NH2 and high-pH anion-exchange chromatography on CarboPac PA1. The purified sialylated oligosaccharides were ... read more analyzed by one-dimensional and two-dimensional 500-MHz 1H-NMR spectroscopy. When necessary, oligosaccharides were treated with endo-beta-galactosidase (and N-acetyl-beta-glucosaminidase) followed by 1H-NMR analysis of the incubation products, to obtain additional structural information. Di-, tri-, tri- and tetraantennary, N-acetyllactosamine-type oligosaccharides occur which can be completely (major) or partially (minor) sialylated. Three different types of alpha2-3-linked sialic acids are present, namely, N-acetylneuraminic acid (95%), ...
Targeting tumor-associated carbohydrate antigens: a phase I study of a carbohydrate mimetic-peptide vaccine in stage IV breast cancer subjects
This application note describes a rapid and robust high-performance anion-exchange chromatography with pulsed amperometric detection method for the accurate determination of common sugars in acid-hydrolyzed biomass samples with high carbohydrate concentrations.
The oligosaccharide chains of microheterogeneous bovine pancreatic DNAases were characterized by the lectin-nitrocellulose sheet method. The active fractions of the DNAases from column chromatography showed four major and several minor spots on a two-dimensional polyacrylamide gel. They were transferred on to nitrocellulose sheets and treated with glycosidases (neuraminidase, endo-beta-N-acetyl glucosaminidase H or F, or peptide N-glycosidase F) and treated with peroxidase-coupled lectins (concanavalin A, Ricinus communis agglutinin or wheat-germ agglutinin). From the results, the most probable oligosaccharide types were proposed to be as follows: the four major spots contained components which had high-mannose type or hybrid-type oligosaccharides, such as those susceptible to endo-beta-N-acetylglucosaminidase H. In addition, spot 1 contained a complex-type biantennary oligosaccharide without sialic acid and spot 3 contained a tri- or tetra-antennary complex-type oligosaccharide with sialic ...
Carbohydrate antigens recognized by natural or preformed and elicited antibodies are central to transplantation/transfusion rejection across ABO blood group and species (xenotransplantation) barriers and are also promising candidates for cancer immunotherapy (Ramsland 2005). The key carbohydrate determinants (epitopes) recognized by antibodies are synthesized by a series of intracellular glycosyltransferases and are expressed on the surface of cells as glycolipids and glycoproteins. Often the minimal carbohydrate epitopes are located at the terminal end of more complex oligosaccharide chains, which result in these epitopes being displayed at a wide range of surface densities and contexts (e.g., glycolipids or glycoproteins). For example, many tumor-associated carbohydrate antigens are broadly expressed at very high densities on the cell surface of primary and metastatic tumors, but the same carbohydrates occur at much lower levels and are typically restricted to a few cell types in healthy ...
There are a large number of enzymes that are capable of modifying carbohydrates or carbohydrate derivatives, and that may be used in various analytical methods. The hydrolytic enzymes, which break glycosidic linkages, are useful in the study of disaccharide or polysaccharide structure and in methods for quantitation (Table 9.2). Such enzymes will hydrolyse the glycosidic linkages between the monosaccharide residues and release the individual components for further analysis. The enzyme is chosen bearing in mind the nature of the glycosidic linkage involved, which may not be unique to one particular disaccharide or polysaccharide. Thus a-glucosidase will hydrolyse both the a(l-»4) linkage of maltose and the a( 1-»2) linkage of sucrose, resulting in the release of glucose in both cases.. ...
Export GlyConnect composition list related to tissue with id 363 Hex:1 HexNAc:2 # id : 495 / avg mass :586.5477 Hex:3 HexNAc:2 # id : 507 / avg mass :910.8325 Hex:3 HexNAc:2 dHex:1 # id : 740 / avg mass :1056.9755 Hex:3 HexNAc:3 # id : 574 / avg mass :1114.0275 Hex:3 HexNAc:3 dHex:1 # id : 202 / avg mass :1260.1705 Hex:3 HexNAc:4 # id : 500 / avg mass :1317.2225 Hex:3 HexNAc:4 NeuAc:1 # id : 64 / avg mass :1608.4804 Hex:3 HexNAc:4 dHex:1 # id : 742 / avg mass :1463.3655 Hex:3 HexNAc:4 dHex:1 NeuAc:1 # id : 328 / avg mass :1754.6234 Hex:3 HexNAc:6 dHex:1 NeuAc:2 # id : 624 / avg mass :2452.2713 Hex:4 HexNAc:2 # id : 652 / avg mass :1072.9749 Hex:4 HexNAc:2 dHex:1 # id : 345 / avg mass :1219.1179 Hex:4 HexNAc:3 # id : 452 / avg mass :1276.1699 Hex:4 HexNAc:3 dHex:1 # id : 713 / avg mass :1422.3129 Hex:4 HexNAc:3 dHex:1 NeuAc:1 # id : 286 / avg mass :1713.5708 Hex:4 HexNAc:4 # id : 648 / avg mass :1479.3649 Hex:4 HexNAc:4 NeuGc:1 # id : 133 / avg mass :1786.6222 Hex:4 HexNAc:4 NeuAc:1 # id : 547 / ...
Export GlyConnect composition list related to peptides with id 1571 Hex:3 HexNAc:2 dHex:1 # id : 740 / avg mass :1056.9755 Hex:3 HexNAc:3 dHex:1 # id : 202 / avg mass :1260.1705 Hex:3 HexNAc:3 dHex:2 # id : 357 / avg mass :1406.3135 Hex:3 HexNAc:4 dHex:1 # id : 742 / avg mass :1463.3655 Hex:3 HexNAc:5 dHex:1 # id : 538 / avg mass :1666.5605 Hex:4 HexNAc:2 dHex:1 # id : 345 / avg mass :1219.1179 Hex:4 HexNAc:3 dHex:1 # id : 713 / avg mass :1422.3129 Hex:4 HexNAc:3 dHex:1 NeuAc:1 # id : 286 / avg mass :1713.5708 Hex:4 HexNAc:3 dHex:2 # id : 160 / avg mass :1568.4559 Hex:4 HexNAc:3 dHex:3 # id : 985 / avg mass :1714.5989 Hex:4 HexNAc:4 NeuAc:1 # id : 547 / avg mass :1770.6228 Hex:4 HexNAc:4 dHex:1 # id : 350 / avg mass :1625.5079 Hex:4 HexNAc:4 dHex:1 NeuAc:1 # id : 173 / avg mass :1916.7658 Hex:4 HexNAc:4 dHex:2 # id : 213 / avg mass :1771.6509 Hex:4 HexNAc:4 dHex:2 NeuAc:1 # id : 685 / avg mass :2062.9088 Hex:4 HexNAc:4 dHex:3 # id : 751 / avg mass :1917.7939 Hex:4 HexNAc:6 # id : 53 / avg mass ...
Read independent reviews on Thermo Scientific™ CarboPac® PA1 Carbohydrate Column from Thermo Fisher Scientific on SelectScience
UPLC, UHPLC, and HPLC-based C18 columns that assist scientists with the analysis and quantitation of important glycan derived components.
Heparin Hexasaccharide MS Standard 7. The Heparin Hexasaccharide MS Standard 7 is a MS standard of defined sequence and structure. This highly purified hexasaccharide with seven sulfates is prepared from a digest of porcine mucosal heparin using Bacteroides Heparinase I
Oxygen is the most abundant element in the planet, so it is not unreasonable to expect that it might be one of the dominant light elements in the core.. The composition of the Earths core remains a mystery. Scientists know that the liquid outer core consists mainly of iron, but it is believed that small amounts of some other elements are present as well.. Now, a new research from a team including Yingwei Fei from Carnegies Geophysical Laboratory has revealed that oxygen does not have a major presence in the outer core.. This has major implications for our understanding of the period when the Earth formed through the accretion of dust and clumps of matter.. According to current models, in addition to large amounts of iron, the Earths liquid outer core contains small amounts of so-called light elements, possibly sulfur, oxygen, silicon, carbon, or hydrogen.. The team provides new experimental data that narrow down the identity of the light elements present in Earths outer core.. High-speed ...
H. Kunz, B. Liebe, W. Dippold, C. Claus, J. Habermann, U. Sprengard, K. Peilstöcker, M. Rösch, in: Medicinal Chemistry into the Millennium (M. M. Campbell, I. S. Blagbrough, Hrsg.), The Royal Society of Chemistry, Cambridge, UK, 2001, 245-257. 263.Applications of Ugi Reactions in Synthesis of Divalent Neoglycoconjugates: Evidence that the Sugars are Presented in Testricted Conformation ...
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
Supplier pricing and chemical structure of high purity Man(a1-2)-Man(a1-3)-[Man(a1-6)-Man(a1-6)]-Man(b1-4)-GlcNAc(b1-4)-GlcNAc from Omicron Biochemicals, Inc.
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the glycosyltransferase 31 family. The encoded protein synthesizes GalNAc:beta-1,3GlcNAc, a novel carbohydrate structure, on N- and O-glycans. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Mar 2013 ...
GlycanMass is a tool which allows to calculate the mass of an oligosaccharide structure [Mass values / Disclaimer]. Note: You can use GlycoMod to predict the possible oligosaccharide structures that occur on proteins from their experimentally determined masses ...
Visit the page of myhomeworkhelp.com and check out their Oligosaccharides Assignment Help team which is ready 24/7 to provide assistance to students.
A. During early development, a snake embryo was exposed to a toxin that interferes with normal migration and differentiation of mesoderm. Name the major structures of the integument most affected by this exposure and how each.
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By the end of this section, you will be able to: Identify the anatomical and functional divisions of the nervous system Relate the functional and struc
Hello everyone, this is part 2 of this series. In the previous part, we discussed how to create a new project and set the home page. In this part, we'll discuss how to perform navigation in Xamarin forms.
Roundtables are designed to encourage scholarly discussion and exchange of views around a subject of relevance and should have a minimum of three presenters, including a chair.. Please provide a single abstract for the roundtable of no more than 200 words including the following structure:. ...
இயற்கையில் இம்மெத்தாக்சி சேர்மங்கள் அதிகமாக காணப்படாமல் இருப்பதாலேயே முக்கியத்துவம் பெறுகின்றன. அவை அமினோ அமிலங்களில் இருப்பதில்லை. இந்த வேற்றணு வளையச் சேர்மங்கள் மரபுக்குறியீட்டிலும் காணப்படவில்லை கொழுமியத்திலும் காணப்படவில்லை. இருந்தாலும் இவை O-மெத்திலேற்ற பிளவோனாய்டு எனப்படும் தாவர நிறமிகளின் பகுதிப்பொருளாக உள்ளது. இவை பீனால்கள் மீது செயல்படுகின்றன. உதாரணம்: ...
Phospholipid patterns of 15 representative strains of the genus Amycolatopsis were recorded by two-dimensional thin-layer chromatography. The structure analysis of the isolated phospholipids was verified by fast atom bombardment-mass spectroscopy. The positive- and negative-ion spectra of the partially purified phospholipid fractions qualitatively reflect their distinctive composition. All strains contained diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylinositol. Two different types of phosphatidylethanolamine and phosphatidylmethylethanolamine were detected, viz., compounds with or without hydroxy fatty acids. These phospholipid patterns underline the integrity of the genus. Fast atom bombardment-mass spectrometry analysis of phospholipid patterns may serve as an aid for differentiation of bacterial species.
TY - JOUR. T1 - Chemical reactions in fast atom bombardment mass spectrometry. AU - Vékey, K.. AU - Zerilli, Luigi F.. PY - 1991. Y1 - 1991. N2 - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time behaviour and in one case it was possible to determine rate constants. These data suggest that chemical reactions can be accelerated significantly by fast atom bombardment.. AB - Examples of various chemical reactions occurring in the matrix or in the selvedge region in fast atom bombardment (FAB) mass spectrometry are discussed. These are categorized as oxidations and reductions; substitutions; clusterings and additions; and sample decomposition or transformation. Some reactions observed showed significant time ...
Equine chorionic gonadotropin (eCG) and lutropin (eLH) are heterodimeric glycoprotein hormones which are synthesized in the placenta and pituitary, respectively. The beta subunits of eCG and eLH, like their alpha subunits, arise from a single gene and have identical amino acid sequences. In contrast, the beta subunits of CG and LH in primates arise from different genes and differ in sequence. We have examined the structures of the Asn-linked oligosaccharides on eCG and eLH. eCG bears di- and tri-branched Asn-linked oligosaccharides terminating with Sia alpha 2,3 or 6Gal beta 1,4GlcNAc. In contrast, , 72% of the Asn-linked oligosaccharides on eLH have 1 or 2 branches terminating with the sequence SO4-4-GalNAc beta 1,4GlcNAc. The nonsulfated oligosaccharides on eLH are neutral (6% of the total) or have branches terminating with sialic acid-Gal (22% of the total). Since the alpha and beta subunits of eCG and eLH both contain the tripeptide motif, Pro-Xaa-Arg/Lys, recognized by the glycoprotein ...
Brown, Gavin M. and Huckerby, Thomas N. and Morris, Haydn G. and Abram, Beverly L. and Nieduszynski, Ian A. (1994) Oligosaccharides Derived from Bovine Articular Cartilage Keratan Sulfates after Keratanase II Digestion: Implications for Keratan Sulfate Structural Fingerprinting. Biochemistry, 33 (16). pp. 4836-4846. ISSN 1520-4995 Full text not available from this repository ...
Heparin and heparan sulfate bind a host of basic proteins that take advantage of the sugars dense structural information. The significance of these interactions in various aspects of development, physiology, and disease stimulated keen interest in evaluating structure-activity relationships. The well-defined heparin and heparan sulfate oligosaccharides needed for these studies can be mainly accessed by chemical synthesis and, more recently by chemoenzymatic means. The various synthetic strategies available to chemical synthesis have recently enabled the acquisition of several regular and irregular sequences, including a number of dodecasaccharides, through improved coupling methods and judicial protecting group manipulations. Controlled chain elongation and critical application of modification enzymes allowed the generation of well-defined constructs via chemoenzymatie synthesis. Investigations of various protein interactions with the synthetic constructs delivered valuable informatio
Identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the O-specific polysaccharides of Klebsiella pneumoniae O3, Hafnia alvei PCM 1223 and Escherichia coli O9/O9a LPS
The fast atom bombardment (FAB) mass spectra of bouvardin (1) 6-O-methylbouvardin (2), deoxybouvardin (3) and a synthetic analog (4) have been examined. The spectra of the bicyclic compounds 1-3 display site-directed fragmentations resulting from the presence of a phenolic bridged tyrosine moiety un …
We have analyzed the O-antigen polysaccharide of the previously uncharacterized Escherichia coli strain TD2158 which is a host of bacteriophage HK620. This bacteriophage recognizes and cleaves the polysaccharide with its tailspike protein (TSP). The polysaccharide preparation as well as oligosaccharides obtained from HK620TSP endoglycosidase digests were analyzed with NMR spectroscopy. Additionally, sugar analysis was performed on the O-antigen polysaccharide and MALDI-TOF MS was used in oligosaccharide analysis. The present study revealed a heterogeneous polysaccharide with a hexasaccharide repeating unit of the following structure: alpha-D-Glcp-(1 -, 6) vertical bar vertical bar 2)-alpha-L-Rhap-(1 -, 6)-alpha-D-Glcp-(1 -, 4)-alpha-D-Galp-(1 -, 3)-alpha-D-GlcpNAc- (1 -,vertical bar beta-D-Glcp/beta-D-GlcpNAc-(1 -, 3) A repeating unit with a D-GlcNAc substitution of D-Gal has been described earlier as characteristic for serogroup O18A1. Accordingly, we termed repeating units with D-Glc ...
Eleven tested anions were able to form adducts with neutral oligosaccharides at low cone voltage in negative ion mode electrospray mass spectrometry. Among them, fluoride and acetate have the abilities to significantly enhance the absolute abundance of [M-H]- for neutral oliogosaccharides. The chloride adduct has the best stability among all the adduct species investigated. For the above three anions, CID of adduct species may be used for structural determination of neutral oligosaccharides. In the presence of F- and Ac-, simultaneous detection of acidic oligosaccharides and neutral oligosaccharides was achieved. The ratio of Cl- : non-Cl-containing product ions obtained in CID spectra of chloride adducts of disaccharides was used to differentiate anomeric configurations of disaccharides. Density functional theory (DFT) was employed to evaluate the optimized structures of chloride adducts of disaccharides. The formation and decomposition of chloride adducts with oligosaccharides of different
N-Acetylglucosaminyltransferase III/MGAT3 products available through Novus Biologicals. Browse our N-Acetylglucosaminyltransferase III/MGAT3 product catalog backed by our Guarantee+.
Purchase Neoglycoconjugates, Part B: Biomedical Applications, Volume 247 - 1st Edition. Print Book & E-Book. ISBN 9780121821487, 9780080883601
article{fb86bc26-01e6-493b-b144-14ab87d1a4c7, abstract = {The synthesis of the Linear B type 2 trisaccharide (Galalpha3Galbeta4GlcNAc-betaOTMSEt) and the corresponding 2-carboxyethyl beta-thioglycoside is described, as well as coupling of the latter to Sepharose.}, author = {Dahmen, J and Magnusson, Göran and Hansen, HC}, issn = {0732-8303}, keyword = {xenotransplantation,glycoconjugate,glycoside synthesis,thioglycoside}, language = {eng}, number = {3}, pages = {189--199}, publisher = {Marcel Dekker}, series = {Journal of Carbohydrate Chemistry}, title = {Synthesis of the Linear B type 2 trisaccharide Gal alpha 3Gal beta 4GlcNAc beta OTMSEt, and coupling of the corresponding 2-carboxyethyl beta-thioglycoside to Sepharose}, url = {http://dx.doi.org/10.1081/CAR-120004332}, volume = {21}, year = {2002 ...
The structures of hepta- to tetradeca-saccharides, generated by digestion of wheat-endosperm arabinoxylan with endo-(1-,4)-beta-D-xylanase, and isolated by gel-permeation chromatography on Bio-Gel P-6 and high-performance anion-exchange chromatography with pulsed amperometric detection (h.p.a.e.-p.a.d.), were elucidated using monosaccharide and methylation analysis, f.a.b.-m.s., and 1H-n.m.r. spectroscopy. The structures identified had two branching elements, -,4)[alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-, ... read more and/or -,4)[alpha-L-Araf-(1-,2)][alpha-L-Araf-(1-,3)]-beta-D-Xylp-(1-,, directly connected to each other in all four possible combinations. The h.p.a.e.-p.a.d. elution pattern showed that these combinations are not present in equal amounts. Also, compounds containing two 2,3-branched beta-D-Xylp residues separated by one or two unbranched beta-D-Xylp residues were found, and the presence of a tetradecasaccharide containing three 2,3-branched beta-D-Xylp residues was established. show ...
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf ...
The primary structure of the major surface glycoconjugate of Leishmania donovani parasites, a lipophosphoglycan, has been further characterized. The repeating PO4-6Galp beta 1-4Man disaccharide units, which are a salient feature of the molecule, are shown to terminate with one of several neutral structures, the most abundant of which is the branched trisaccharide Galp beta 1-4(Manp alpha 1-2)Man. The phosphosaccharide core of lipophosphoglycan, which links the disaccharide repeats to a lipid anchor, contains 2 phosphate residues. One of the core phosphates has previously been localized on O-6 of the galactosyl residue distal to the lipid anchor; the second phosphate is now shown to be on O-6 of the mannosyl residue distal to the anchor and to bear an alpha-linked glucopyranosyl residue. Also, the anomeric configuration of the unusual 3-substituted Galf residue in the phosphosaccharide core is established as beta. The complete structure of the core is thus PO4-6Galp alpha 1-6Galp alpha 1-3Galf ...
The oligosaccharides ID in the library are used to refer to specific oligosaccharides for their tandem MS/MS fingerprints and structures and were obtained using their sequential monomeric composition. For example, the oligosaccharide with ID 11100 is made of 1 hexose + 1 HexNAc + 1 fucose + 0 NeuAc + 0 NeuGC.. ...
The Global Soybean Oligosaccharides report provides an elite resource to evaluate the Soybean Oligosaccharides market and support the strategic and preemptive decision-making. The report divulges the matter-of-fact statistics and in-detail examination of the market. It offers a basic layout of the Soybean Oligosaccharides industry, consisting of classifications, applications, definitions, and industry chain structure. It also encompasses a thorough inference of the market and embodies significant insights, facts, and industry-corroborated statistics of the global Soybean Oligosaccharides market.. Furthermore, the report also elaborates several factors concerning the Soybean Oligosaccharides market, comprising key trends, standardization, deployment models, future roadmap, strategies, technologies, value chain, opportunities, ecosystem player profiles, drivers, operator case studies, regulatory landscape, and challenges. Moreover, it also presents an outline regarding the Soybean Oligosaccharides ...
This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein ...
Sialyl Lewis A (sLeA, also known as CA19-9), a tetrasaccharide selectively and highly expressed on advanced adenocarcinomas including colon, stomach, and pancreatic cancers, has long been considered as an attractive target for active and passive vaccination. While progress in antibodies targeting tumor-associated protein antigens resulted in an impressive array of therapeutics for cancer treatment, similar progress in exploiting tumor-associated carbohydrate antigens, such as sLeA, has been hampered by the lack of a detailed understanding of the singular characteristics of these antigens. We have addressed this issue by analyzing antibodies derived from patients immunized with an sLeA/KLH vaccine. These antibodies were engineered to mediate tumor clearance in vivo in preclinical models through Fc-FcγR interactions. However, in contrast to protein antigens in which hFcγRIIIA engagement was both necessary and sufficient to mediate tumor clearance in both preclinical and clinical settings, a ...
Sulfotransferase that utilizes 3-phospho-5-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues and O-linked sugars of mucin-type acceptors. Acts on the non-reducing terminal GlcNAc of short carbohydrate substrates. However, it does not transfer sulfate to longer carbohydrate substrates that have poly-N-acetyllactosamine structures. Has no activity toward keratan. Not involved in generating HEV-expressed ligands for SELL. Its substrate specificity may be influenced by its subcellular location ...
keratanase II: an endo-beta-N-acetylglucosaminidase; cleaves the beta(1-3)-glycosidic bond of a fucosylated 6-O-sulfated N-acetylglucosamine
In the few years since the beginning of glycomics in 21th century, many achievements have been made in the study of glycomics, especially in the discovery of sugar genes and the elucidation of the new structure of sugar chain. Although glycomics research has made remarkable achievements in these fields, there are many unknown intermediate links between sugar genes and sugar chains, sugar chains and glycan binding proteins, and there is a general lack of detailed research on the relationship between structure and function.. These fields are not only related to the progress of glycomics itself, but also directly related to the progress of proteome planning, so it has become a major bottleneck in the development of life science.. The advancement of analytical techniques has always been a direct driving force in the development of glycomics. It is the rapid development and wide application of modern instrument analysis technology that lead to the overall prosperity of glycomics research, and inject ...
The MUC-1 antigen is a membrane-anchored mucin-type glycoprotein present in malignant and normal epithelial cells of certain organs, e.g. breast, lung, ovary, pancreas and colon (1). The apoprotein of the MUC-1 mucin contains a transmembrane domain, a cytoplasmic domain, and an extracellular carbohydrate rich domain. The extracellular domain is characterized by polymorphism with respect to the number of a 20 amino acid tandem repeat (VNTR polymorphism). The CanAg CA15-3 EIA is based on two mouse monoclonal antibodies, Ma695 as catcher antibody recognizing a sialylated carbohydrate epitope expressed on the MUC-1 antigen and Ma552 as tracer antibody targeting the PDTRPAPG region of the protein core (2-5).The MUC-1 breast cancer mucin (CA15-3 antigen) is secreted from tumor cells and is a well-established serological marker for monitoring the clinical course of breast cancer patients (6). ...
Thermo Fisher Scientific has developed a high-performance anion-exchange chromatography with pulsed amperometric detection method, based on AOAC Official Method 2011. Click to read more...
RNase B is a glycoprotein which possesses an amino acid composition indistinguishable from that of RNase A and which contains carbohydrate to the extent of 6 residues of mannose and 2 residues of N-acetylglucosamine per molecule. It is consequently considered to be a carbohydrate derivative of RNase A. ( Tarentino, A., Plummer, J., and Maley, F.: Studies on the Oligosaccharide Sequence of Ribonuclease B, J. Biol. Chem., 245, 4150 (1970 ...
The present study tested and confirmed the hypothesis that autoantibodies to aberrant O-glycopeptide epitopes represent a fruitful source of sensitive biomarkers for early detection of cancer. Cancer-associated IgG autoantibodies to several O-glycopeptide epitopes were identified in MUC1, whereas IgG antibodies to peptide epitopes were not detected. The study therefore clearly supports that autoantibody biomarker discovery strategies should include aberrant posttranslational modifications for greatest success. Chemoenzymatic synthesis of cancer-associated O-glycopeptides in combination with a microarray platform was shown to be a feasible strategy for broader analysis of the entire cancer O-glycopeptidome.. Initially, we found an absence of immature nonsialylated MUC1 and MUC16 glycoforms in serum of cancer patients with elevated mucin levels, suggesting that these glycoforms are removed by immune cells or scavenger receptors recognizing immature uncapped glycans. This is in agreement with Varki ...
An estimate of the relative abundance of the various O-glycans was determined from nanoLC-ESI-IT-MS analysis of trypsin-generated (glyco)peptides. It should be noted that because the signal of the monosialylated O-glycopeptide in triply charged state overlapped with the doubly charged peak of the hinge repeat peptide with a putative acetylation modification in all samples, only the doubly charged signal of the monosialylated O-glycopeptide was quantified, thereby leading to an underestimation of this O-glycoform. Furthermore, it is known that glycopeptides with different glycan structures can have different response factors (35), and thus the relative abundances we measured may not accurately reflect the real ratios. In order to obtain a more reliable estimate of the percentage of the hinge repeat motif bearing an O-glycan, tryptic IgG peptides were incubated with exoglycosidases, trimming all O-glycans down to a single N-acetylhexosamine. A previous study of quantitative measurements of a ...
View Notes - 351 C4 Smith key from CHEM 351 at BYU. Chemistry 351 Fall 2008 Chapter 4 1. Assign the IUPAC name to the following structures. 0k 1,: _Jhfigg~mefi%fimfilm c, 2&4
Mgat4c (untagged) - Mouse mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme C (putative) (Mgat4c), transcript variant 1, (10ug), 10 µg.
IN STOCK 13C labeled di-sialylated, galactosylated biantennary N-Glycan labeled with 2 13C atoms per GlcNAc, for use as a quantitative standard in applications
Kilpatrick, D.C. Pusztai, A., Grant, G., Graham, C. and Ewen, S.W (1985) Tomato Lectin Resists Digestion in the Mammalian Alimentary Canal and Binds to Intestinal Villi without Deleterious Effects. FEBS Letters, 185, 299-305.
EMDEX® is a directly compressible, water-soluble tablet binder and filler. Its unique composition of 95% glucose monohydrate and different oligosaccharides derived from starch is monographed under
Shop Oligosaccharide 4-alpha-D-glucosyltransferase ELISA Kit, Recombinant Protein and Oligosaccharide 4-alpha-D-glucosyltransferase Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC Human Anti-Meningococcal Group A Oligosaccharides IgG negative control serum Serum Controls 600-801-ANC
Background Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X...
Cells All organisms are made of cells. The cell is the smallest unit of living material having major structures within it allowing it to live. Some kinds of organisms are just one cell. This single cell is the organisms entire body. Many organisms are made of more than one cell.
19 janvier 2012. P,The Taleghan fault (TF) is a major active fault of the Central Alborz mountain range in Iran. Located 50 km northwestwards of Tehran, this 80-km-long fault represents one of the major structures threatening 15 million people living in the capital of Iran and the surrounding cities (e.g. (...). Lire la suite. ...
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undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
undecaprenyl phosphate-α-L-Ara4N: 4-amino-4-deoxy-β-L-arabinosyltransferase (EC 2.4.2.43); dodecaprenyl phosphate-β-galacturonic acid: lipopolysaccharide core α-galacturonosyl transferase (EC 2.4.1.- ...
For an organism to develop and function, the individual cells must exchange information, or communicate, with each other. Is it possible to learn their language and
Methods to allow the clean preparation of oligosaccharides were investigated using techniques that do not require conventional column chromatography or an aqueous work-up. The route was designed to provide rapid access to oligosaccharides and is suit
In this 3 volume collection focusing on glycomics, readers will appreciate how such discoveries were made and how such methods can be applied for readers...
In previous studies, we have shown that the highly sialylated renal anti-adhesin Podocalyxin (PC) is expressed in the developing brain [13], [14 ...