As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
AA3 enzymes belong to the glucose-methanol-choline (GMC) oxidoreductases family. AA3 enzymes are flavoproteins containing a flavin-adenine dinucleotide (FAD)-binding domain. Family AA3 can be divided into 4 subfamilies: AA3_1 (mostly cellobiose dehydrogenases), AA3_2 (including both aryl alcohol oxidase and glucose 1-oxidase), AA3_3 (alcohol oxidase) and AA3_4 (pyranose 2-oxidase ...
SWISS-MODEL Template Library (SMTL) entry for 1mfz. Partially refined 2.8 A Crystal structure of GDP-mannose dehydrogenase from P. aeruginosa
Boc Sciences offers cas 18422-53-2 1,2:4,5-Di-O-isopropylidene-b-D-erythro-2,3-hexodiulo-2,6-pyranose in bulk,please inquire us to get a quote for 18422-53-2 1,2:4,5-Di-O-isopropylidene-b-D-erythro-2,3-hexodiulo-2,6-pyranose.
Proliferating cells, including cancer cells, obtain serine both exogenously and via the metabolism of glucose. By catalyzing the first, rate-limiting step in the synthesis of serine from glucose, phosphoglycerate dehydrogenase (PHGDH) controls flux through the biosynthetic pathway for this important amino acid and represents a putative target in oncology. To discover inhibitors of PHGDH, a coupled biochemical assay was developed and optimized to enable high-throughput screening for inhibitors of human PHGDH. Feedback inhibition was minimized by coupling PHGDH activity to two downstream enzymes (PSAT1 and PSPH), providing a marked improvement in enzymatic turnover. Further coupling of NADH to a diaphorase/resazurin system enabled a red-shifted detection readout, minimizing interference due to compound autofluorescence. With this protocol, over 400,000 small molecules were screened for PHGDH inhibition, and following hit validation and triage work, a piperazine-1-thiourea was identified. Following ...
TY - JOUR. T1 - Phosphoglycerate dehydrogenase diverts glycolytic flux and contributes to oncogenesis. AU - Locasale, Jason W.. AU - Grassian, Alexandra R.. AU - Melman, Tamar. AU - Lyssiotis, Costas A.. AU - Mattaini, Katherine R.. AU - Bass, Adam J.. AU - Heffron, Gregory. AU - Metallo, Christian M.. AU - Muranen, Taru. AU - Sharfi, Hadar. AU - Sasaki, Atsuo T.. AU - Anastasiou, Dimitrios. AU - Mullarky, Edouard. AU - Vokes, Natalie I.. AU - Sasaki, Mika. AU - Beroukhim, Rameen. AU - Stephanopoulos, Gregory. AU - Ligon, Azra H.. AU - Meyerson, Matthew. AU - Richardson, Andrea L.. AU - Chin, Lynda. AU - Wagner, Gerhard. AU - Asara, John M.. AU - Brugge, Joan S.. AU - Cantley, Lewis C.. AU - Vander Heiden, Matthew G.. PY - 2011/9/1. Y1 - 2011/9/1. N2 - Most tumors exhibit increased glucose metabolism to lactate, however, the extent to which glucose-derived metabolic fluxes are used for alternative processes is poorly understood. Using a metabolomics approach with isotope labeling, we found that ...
BioAssay record AID 400045 submitted by ChEMBL: Antimicrobial activity against Sporotrichum pulverulentum after 60 mins by agar diffusion method.
It is reasonable that efficient conversion of L-serine to pyruvate requires sufficient availability of L-serine. To enhance the biosynthesis of L-serine, we overexpressed the genes of de novo L-serine biosynthetic pathway. L-serine is synthesized from D-3-phosphoglycerate by three reactions catalyzed by D-3-phosphoglycerate dehydrogenase, D-3-phosphoserine aminotransferase and phosphoserine phosphatase, which are encoded by serA, serC and serB, respectively (Figure 1). D-3-phosphoglycerate dehydrogenase is regulated by allosteric end-product inhibition. Moreover, a published report has showed that a truncated D-3-phosphoglycerate dehydrogenase (PGDH) serA Δ197 was no longer inhibited by L-serine in C. glutamicum [18]. As such, we combined serA Δ197 together with serB and serC into an artificial operon driven by the constitutive promoter trc, creating plasmid pTSer. Strain SD01 was transformed with plasmid pTSer for activating the Serine-Deamination (SD) pathway. After 48h cultivation, 3.96 g/L ...
Carbohydrate dehydrogenases are a group of dehydrogenase enzymes that occur in many organisms and facilitate the conversion from a carbohydrate to an aldehyde, lactone, or ketose.. Carbohydrate dehydrogenases are the most common quinoprotein oxidoreductases,[1] which are enzymes that oxidize a wide range of molecules.. An example includes L-gulonolactone oxidase.. They are categorized under EC number 1.1. More specifically, they are in three subcodes: 1, 2, and 99, categorized as follows:. ...
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TY - JOUR. T1 - Kinetic modeling of a bi-enzymatic system for efficient conversion of lactose to lactobionic acid. AU - Van, Wouter. AU - Bhagwat, Aditya. AU - Ludwig, Roland. AU - Dewulf, Jo. AU - Haltrich, Dietmar. AU - Van Langenhove, Herman. PY - 2009/4/1. Y1 - 2009/4/1. N2 - A model has been developed to describe the interaction between two enzymes and an intermediary redox mediator. In this bi-enzymatic process, the enzyme cellobiose dehydrogenase oxidizes lactose at the C-1 position of the reducing sugar moiety to lactobionolactone, which spontaneously hydrolyzes to lactobionic acid. 2,20 Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt is used as electron acceptor and is continuously regenerated by laccase. Oxygen is the terminal electron acceptor and is fully reduced to water by laccase, a coppercontaining oxidase. Oxygen is added to the system by means of bubble-free oxygenation. Using the model, the productivity of the process is investigated by simultaneous solution ...
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PHGDH antibody [N3C2], Internal (phosphoglycerate dehydrogenase) for IHC-Fr, IHC-P, WB. Anti-PHGDH pAb (GTX101949) is tested in Human, Rat samples. 100% Ab-Assurance.
D-serine is an endogenous ligand for NMDARs generated from L-serine by the enzyme serine racemase (Srr). Both neuronal and glial localizations have been reported for D-serine and Srr. 3-phosphoglycerate dehydrogenase ...
Recognized as one of the 100 most technologically significant products introduced to the marketplace in the past year. The Remote Methane Leak Detector can quickly and efficiently detect leaks up to one hundred feet away. Using laser technology, remote detection allows the user to safely survey areas that may be difficult to reach, such as busy roadways, yards with large dogs, locked gates, pipe suspended under a bridge and other hard to access places. In the independent validation tests, the RMLD has proven to be a highly effective leak survey instrument, compared to flame ionization and similar equipment, but with the added advantage of remote detection. By design the RMLD is capable of achieving significant productivity gains and drastically reduce operations and maintenance costs ...
Hi Vivek, There are a number of hidden parameters changed in 9i that affected CBO (as compared to 8i CBO). I suggest contacting Oracle Support before changing any hidden parameters. I think this is know problem. If possible, test your application with 9.2.0.6. That seems to a stable version of 9i, so far. Regards, - Kirti --- VIVEK_SHARMA ,[email protected], wrote: , , ISSUE - Getting HIGH Latch Free Wait on the following Latches after moving , from RBO to , CBO. ( ALL Objects been analyzed at 100 %). CPU Usage on DB Server has gone , up by about , 30 %. NOTE - Application has also been migrated to a Higher release along , with the CBO , movement. , , Qs Any init.ora parameters to Tune ? , , Would increasing _shared_pool_reserved_min_alloc to 6140 from the Default of , 4400 Help? , , Setting cursorsharing = FORCE/SIMILAR caused %sys component of CPU Usage to , shoot to , 99 % within minutes of Database startup. Seemed to be hitting some Bug in , 9.2.0.5 (64 , Bit) on Solaris 9. Has ...
When determining the effects on the deficit of a certain legislative action, both revenues and spending have to be accounted for. Indeed, you cant determine
Neu-Laxova syndrome (NLS) is an autosomal recessive disorder characterized by severe congenital malformations leading to prenatal or early postnatal lethality. Main findings include intrauterine growth retardation, microcephaly, ichthyosis, flexion deformities, edema of the hands and feet, and abnormal facial features including abnormal or absent eyelids, flat or abnormal nose, and a round gaping mouth. NLS1 (MIM 256520) and NLS2 (MIM 616038) are caused by mutations in the PHGDH and PSAT1 genes. They code for D-3-phosphoglycerate dehydrogenase and phosphoserine aminotransferase enzymes, respectively. These enzymes are involved in L-serine biosynthesis. Phosphoglycerate dehydrogenase deficiency (PHGDHD; MIM 601815) and phosphoserine aminotransferase deficiency (PSATD; MIM 610992) are autosomal recessive disorders allelic to more severe disorders, NLS1 and NLS2. PHGDHD and PSATD are characterized by congenital microcephaly, hypertonia, psychomotor retardation and seizures.. Read less ...
The PHGDH gene provides instructions for making the parts (subunits) that make up the phosphoglycerate dehydrogenase enzyme. Four PHGDH subunits combine to form the enzyme. This enzyme is involved in the production (synthesis) of the protein building block (amino acid) serine. Specifically, the enzyme converts a substance called 3-phosphoglycerate to 3-phosphohydroxypyruvate in the first step in serine production. Serine is necessary for the development and function of the brain and spinal cord (central nervous system). Serine is a part of chemical messengers called neurotransmitters that transmit signals in the nervous system. Proteins that form cell membranes and the fatty layer of insulation (myelin) that surrounds many nerves also contain serine.. Serine can be obtained from the diet, but brain cells must produce their own serine because dietary serine cannot cross the protective barrier that allows only certain substances to pass between blood vessels and the brain (the blood-brain barrier). ...
The sugar oxidising enzymes glucose oxidase, glucose dehydrogenases (GDH) and cellobiose dehydrogenases (CDH) were co-immobilised, in the presence of multiwalled carbon nanotubes, with osmium redox polymers. Under pseudo-physiological conditions of 5 mM glucose, 150 mM NaCl, 37?degrees C, glucose oxidation current densities above 800 mu A?cm-2 are obtained from films containing an [Os(4,4xxx-dimethyl-2,2xxx-bipyridine)2(poly-vinylimidazole)10Cl]+ redox polymer, redox potential 0.1 V vs. Ag/AgCl, and either glucose oxidase or FAD-dependant GDH. Current produced by, and stability of, glucose-oxidising half-cells is compared in 100 mM glucose, with films containing CDHs proving most stable. Such results show promise for development of glucose-oxidising enzymatic fuel cells ...
Pyranose Oxidase antibody LS-C744693 is an unconjugated goat polyclonal antibody to Pyranose Oxidase from e. coli. It is reactive with bacteria and e. coli. Validated for ELISA, IP and WB.
This Research Topic addresses the metabolism and function of the amino acid Serine in plants. We emphasize the interaction and coordination between the Serine biosynthetic pathways and other metabolic pathways.Serine is a polar amino acid that plays a fundamental role in plant metabolism, plant development, and cell signalling. In addition to being a building block for proteins, Serine participates in the biosynthesis of biomolecules such as amino acids, nucleotides, phospholipids, and sphingolipids. Plants possess at least two serine biosynthetic pathways: i) the glycolate pathway associated with photorespiration and ii) the so-called Phosphorylated Pathway of Serine Biosynthesis. The biological significance of the coexistence of several pathways for the biosynthesis of Serine is not known. In particular, we poorly understand the contribution that each pathway makes to plant serine homeostasis, how pathways are integrated and coordinated, and how they interact at the transcriptional/translational
Blog on Phgdh elisa kit product: The Mouse Phgdh phgdh (Catalog #MBS2602994) is an ELISA Kit and is intended for research purposes only. T...
ID E7A1S5_SPORE Unreviewed; 226 AA. AC E7A1S5; DT 08-MAR-2011, integrated into UniProtKB/TrEMBL. DT 08-MAR-2011, sequence version 1. DT 25-OCT-2017, entry version 22. DE SubName: Full=Uncharacterized protein {ECO:0000313,EMBL:CBQ73432.1}; GN ORFNames=sr14090 {ECO:0000313,EMBL:CBQ73432.1}; OS Sporisorium reilianum (strain SRZ2) (Maize head smut fungus). OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Sporisorium. OX NCBI_TaxID=999809 {ECO:0000313,EMBL:CBQ73432.1, ECO:0000313,Proteomes:UP000008867}; RN [1] {ECO:0000313,EMBL:CBQ73432.1, ECO:0000313,Proteomes:UP000008867} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=SRZ2 {ECO:0000313,Proteomes:UP000008867}; RX PubMed=21148393; DOI=10.1126/science.1195330; RA Schirawski J., Mannhaupt G., Muench K., Brefort T., Schipper K., RA Doehlemann G., Di Stasio M., Roessel N., Mendoza-Mendoza A., RA Pester D., Mueller O., Winterberg B., Meyer E., Ghareeb H., RA Wollenberg T., ...
The following are the more stable anomers of the pyranose forms of d-glucose, d-mannose, and d-galactose: O HO HO OH OH O HO HO HO OH OH OH O HO HO OH OH -D-Glucopyranose (64% at equilibrium) -D-Mannopyranose (68% at equilibrium) -D-Galactopyranose (64% at equilibrium) OH On the basis of these empirical observations
An improved method is presented for the purification of 8 α-(N1-histidyl)riboflavin, 8 α-(N3-histidyl)riboflavin and their 2′,5′-anhydro forms, which permits the isolation of sizeable quantities of each of these compounds from a synthetic mixture in pure form. Flavin peptides were isolated from the D-gluconate dehydrogenases of Pseudomonas aeruginosa and Pseudomonas fluorescens and from the 2-keto-D-gluconate dehydrogenase of Gluconobacter melanogenus. After conversion into the aminoacyl-riboflavin, the flavin in all three enzymes was identified as 8 α-(N3-histidyl)riboflavin. By sequential treatment with nucleotide pyrophosphatase and alkaline phosphatase, the flavin in each enzyme was shown to be in the dinucleotide form. ...
Biomass is constructed of dense recalcitrant polymeric materials: proteins, lignin, and holocellulose, a fraction constituting fibrous cellulose wrapped in hemicellulose-pectin. Bacteria and fungi are abundant in soil and forest floors, actively recycling biomass mainly by extracting sugars from holocellulose degradation. Here we review the genome-wide contents of seven Aspergillus species and unravel hundreds of gene models encoding holocellulose-degrading enzymes. Numerous apparent gene duplications followed functional evolution, grouping similar genes into smaller coherent functional families according to specialized structural features, domain organization, biochemical activity, and genus genome distribution. Aspergilli contain about 37 cellulase gene models, clustered in two mechanistic categories: 27 hydrolyze and 10 oxidize glycosidic bonds. Within the oxidative enzymes, we found two cellobiose dehydrogenases that produce oxygen radicals utilized by eight lytic polysaccharide ...
Pyranose is a collective term for saccharides that have a chemical structure that includes a six-membered ring consisting of five carbon atoms and one oxygen atom. There may be other carbons external to the ring. The name derives from its similarity to the oxygen heterocycle pyran, but the pyranose ring does not have double bonds. A pyranose in which the anomeric OH at C(l) has been converted into an OR group is called a pyranoside. The pyranose ring is formed by the reaction of the hydroxyl group on carbon 5 (C-5) of a sugar with the aldehyde at carbon 1. This forms an intramolecular hemiacetal. If reaction is between the C-4 hydroxyl and the aldehyde, a furanose is formed instead. The pyranose form is thermodynamically more stable than the furanose form, which can be seen by the distribution of these two cyclic forms in solution. Hermann Emil Fischer won the Nobel Prize in Chemistry (1902) for his work in determining the structure of the D-aldohexoses. However, the linear, free-aldehyde ...
1H6B: Crystal Structures of the Precursor Form of Glucose-Fructose Oxidoreductase from Zymomonas Mobilis and its Complexes with Bound Ligands
Holzer, H. & Holldorf, A. (1957). „Isolation of a D-glycerate dehydrogenase, its properties, and its use for the optical determination of hydroxypyruvate in the presence of pyruvate". Biochem. Z. 329: 292-312. PMID 13522707 ...
Treatment for Cortisone Reductase Deficiency in Sewri West, Mumbai. Find Doctors Near You, Book Appointment, Consult Online, View Doctor Fees, Address, Phone Numbers and Reviews. Doctors for Cortisone Reductase Deficiency in Sewri West, Mumbai | Lybrate
Treatment for Cortisone Reductase Deficiency. Find Doctors Near You, Book Appointment, Consult Online, View Doctor Fees, Address, Phone Numbers and Reviews. Doctors for Cortisone Reductase Deficiency | Lybrate
Influence of Carbon Source on the Production of Extracellular Ligninolytic Enzymes by Phanerochaete chrysosporium. Fangfang Wang,a Mingqiang Ai,a Guihua Yang,b Jiachuan Chen,b Xiulan Chen,a and Feng Huang a,*. The effect of altering the carbon source in the growing environment was investigated relative to the production of ligninolytic enzymes by Phanerochaete chrysosporium. Glucose, cellobiose, and cellulose (or mixtures thereof) were used as the carbon sources. Glucose oxidase and glyoxal oxidase activities in all carbon sources were produced during cultivation. High peak levels (0.17 to 0.24 IU/mL) of manganese peroxidase activity were observed only in mediums containing oligosaccharides. Lignin peroxidase activity was high in glucose medium (0.21 IU/mL of peak value); however, minimal amounts were formed in the cellulose medium (0.01 IU/mL of peak value). High amounts of cellobiose:quinone oxidoreductase (3.33-3.99 IU/mL of peak value) and cellobiose dehydrogenase (0.04-0.2 IU/mL of peak ...
Effects of kraft pulp and lignin on Trametes versicolor carbon metabolism. Purification and characterization of cellobiose dehydrogenases from the white rot fungus Trametes versicolor
Enzyme with hydroxy-pyruvate reductase, glyoxylate reductase and D-glycerate dehydrogenase enzymatic activities. Reduces hydroxypyruvate to D-glycerate, glyoxylate to glycolate oxidizes D-glycerate to hydroxypyruvate.
Isolation and purification of Pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation
Published in the January 2001 Issue of Anvil Magazine Note: Images with captions are included at the end of this article. "Scratches" is a term that refers to a skin problem on the lower legs of horses, caused by a fungus (and sometimes complicated by bacteria). The affected area becomes crusted, scabby and thickened, creating bumps and sometimes open sores. In severe cases the affected skin may ooze or the whole lower leg may swell, and the horse may become lame. This skin condition generally affects unpigmented skin (the areas of white leg markings) more readily than dark skin, since the unpigmented skin is not as tough-and more apt to chaff and scrape, opening the way for infection. Scratches is a dermatitis, or inflammation of the skin, and the most common cause seems to be the fungus Sporotrichum schenki. Some horses seem to be more susceptible than others, just as some seem more vulnerable to other fungal infections such as ringworm and girth itch. The fungus lives in organic matter and ...
A glucuronoyl esterase (GE) from the thermophilic fungus Sporotrichum thermophile, belonging to the carbohydrate esterase family 15 (CE-15), was functionally expressed in the methylotrophic yeast Pich
A glycoprotein containing one mole of FAD per mole of enzyme. 2,6-Dichloroindophenol can act as acceptor. cf. EC 1.1.5.2, quinoprotein glucose dehydrogenase ...
Wolbachia sp. subsp. Drosophila simulans UDP-N-acetylenolpyruvoylglucosamine reductase (murB) datasheet and description hight quality product and Backed by our Guarantee
NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 201.9) ...
NADP_Rossmann (CL0063) 2-Hacid_dh_C; D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain (PF02826; HMM-score: 180.6) ...
Quinoprotein glucose dehydrogenase (EC 1.1.99.17) from Acinetobacter calcoaceticus L.M.D. 79.41 was purified to homogeneity. It is a basic protein with an isoelectric point of 9.5 and an Mr of 94,000. Denaturation yields two molecules of PQQ/molecule and a protein with an Mr of 48000, indicating that the enzyme consists of two subunits, which are probably identical because even numbers of aromatic amino acids were found. The oxidized enzyme form has an absorption maximum at 350 nm, and the reduced form, obtained after the addition of glucose, at 338 nm. Since double-reciprocal plots of initial reaction rates with various concentrations of glucose or electron acceptor show parallel lines, and substrate inhibition is observed for glucose as well as for electron acceptor at high concentrations, a ping-pong kinetic behaviour with the two reactants exists. From the plots, Km values for glucose and Wursters Blue of 22 mM and 0.78 mM respectively, and a Vmax. of 7.730 mumol of glucose oxidized/min per ...
The practice of exposing liquid cultures of the white-rot fungus Phanerochaete chrysosporium to a pure oxygen atmosphere under conditions of nutrient starvation has been widely adopted to induce lignin peroxidase (LiP) synthesis. Transmission electron microscopy was used to examine hyphal cells of carbon-limited cultures that had been exposed to an atmosphere of pure oxygen, and revealed evidence of a major loss in organization of cellular ultrastructure, which may be attributed to oxygen toxicity. Under some conditions (continuous agitation in air with cellulose as the carbon source) cultures will produce LiP without needing to be exposed to a pure oxygen atmosphere. A similar major loss of cellular ultrastructure was found in hyphal cells from such cultures upon examination. Investigation of the levels of H2O2, catalase and carbonyl content of intracellular proteins suggests that the latter cultures developed a hyperoxidant state because the rate of supply of carbon from cellulose hydrolysis was
This paper reports the isolation of phenoloxidase-negative mutants of the white-rot fungus Phanerochaete chrysosporium and the results of a survey of idiophasic functions among these mutants. The mutant strains were isolated from a medium containing o-anisidine after gamma irradiation of wild-type spores and fell into four classes, divided by the manner in which they mineralized 14C-lignin wheat lignocellulose. Examples are strain LMT7, which degraded lignin at a rate similar to that of the wild type; strain LMT26, in which degradation was enhanced; strain LMT16, whose degradation rate was apparently unaffected, although the onset of lignin attack was delayed compared with that in the wild type; and strain LMT24, which was unable to evolve significant amounts of 14CO2 from the radiolabeled substrate. The mutants were not necessarily defective in other functions associated with idiophasic activities (intracellular cyclic AMP levels, sporulation, extracellular glucan production, veratryl alcohol ...
Myc transcriptionally regulates genes involved in processes such as cell proliferation, metabolism, differentiation, and angiogenesis. MYC expression is deregulated in many types of human cancer; therefore discovering the mechanisms behind MYCs role in tumorigenesis is essential. In this dissertation, I have focused on several Myc target genes, Spermidine synthase (Srm); Lactate dehydrogenase (Ldh); 3-phosphoglycerate dehydrogenase (Phgdh); Serine hydroxymethyltransferase (SHMT) 1 and 2; and Pim-3 (a member of the Pim family of serine/threonine kinases). These enzymes play a role in various functions: Spermidine synthase (polyamine synthesis); Lactate dehydrogenase (glycolysis); Phgdh and Shmt (serine metabolism); and Pim-3 (cell signaling). In order to elucidate the impact Myc over-expression has on metabolism in tumorigenesis, we use human cell lines, and transgenic mice as well as cell lines and tissues derived from these mice. The impact of inhibition of these target genes on Myc-driven ...
1. Two enzymes that catalyse the reduction of glyoxylate to glycollate have been separated and purified from a species of Pseudomonas. Their molecular weights were estimated as 180000. 2. Reduced nicotinamide nucleotides act as the hydrogen donators for the enzymes. The NADH-linked enzyme is entirely specific for its coenzyme but the NADPH-linked reductase shows some affinity towards NADH. 3. Both enzymes convert hydroxypyruvate into glycerate. 4. The glyoxylate reductases show maximal activity at pH6.0-6.8, are inhibited by keto acids and are strongly dependent on free thiol groups for activity. 5. The Michaelis constants for glyoxylate and hydroxypyruvate were found to be of a high order. 6. The reversibility of the reaction has been demonstrated for both glyoxylate reductases and the equilibrium constants were determined. 7. The reduction of glyoxylate and hydroxypyruvate is not stimulated by anions.. ...
article{31e17f38-485f-4324-be67-f72cc473b767, abstract = {Both the antibody affinity and the detectability of the label are essential in deciding the final characteristics of a heterogeneous immunoassay. This paper describes an approach to obtain a supplementary enhancement of the signal generated by using an enzyme label, e.g., by including the product of the enzymatic reaction in an additional amplification cycle during the detection step performed with an amperometric biosensor based on glucose dehydrogenase (GDH). An immunoassay format with a labelled analyte derivative that competes with the analyte present in the sample for a limited amount of antibody binding sites was employed. The beta-galactosidase label hydrolyses the substrate aminophenyl-beta-galactopyranoside, and the generated aminophenol enters then into a bioelectrocatalytic amplification cycle at the GDH biosensor. The principle was applied for determination of 4-nitrophenol, with the best minimal concentration of 1.5 microM ...
Strain YM16-304T lacks the dapE gene for succinyl-diaminopimelate desuccinylase (EC:3.5.1.18) in the biosynthesis pathway of lysine and diaminopimelic acids (DAPs). Instead, two candidate genes (YM304_26990 and YM304_19190) for LL-DAP aminotransferase (EC:2.6.1.83, dapL), that constitutes an alternative DAP-lysine biosynthesis pathway (DAP aminotransferase pathway [24,25]), were identified. The dapL gene is found in discrete lineages of Bacteria and Archaea, and is known to complement Escherichia coli dapD and dapE mutants, although purified proteins favor the reverse reaction rather than the synthesis of LL-DAP [25].. Among the genes of serine biosynthesis pathway, the serB gene for phosphoserine phosphatase (EC:3.1.3.3) was not identified by similarity searches. On the other hand, the thrH gene for phosphoserine / homoserine phosphotransferase [26] (EC:3.1.3.3, 2.7.1.39) was identified (YM304_28950). The possibility of using thrH gene product for serine biosynthesis instead of serB gene ...
Acer campestre is a slow-growing deciduous tree with dark, oval leaves that turn yellow in the autumn. The 5 lobed leaves are composed of 3 to 5 entire lobes. Blooms in corymbs of 5 green flowers followed by winged fruit. The cultivar, Pulverulentum is a small tree to 12 feet tall with a large crown, wider than