Campylobacter fetus subsp. venerealis is an important venereal pathogen. We sequenced the genomes of Campylobacter fetus subsp. venerealis bv. venerealis strain B6 and bv. intermedius strain 642-21. The genetic variability of these Australian strains will facilitate the study of mechanisms of geographical adaptation of these pathogens that impact livestock.. ...
Looking for Campylobacter fetus? Find out information about Campylobacter fetus. A genus of bacteria in the family Spirillaceae; spirally curved rods that are motile by means of a polar flagellum at one or both poles Explanation of Campylobacter fetus
Domain architectures containing the following SCOP superfamilies 51419 in Campylobacter fetus subsp. fetus 82-40. Domain architectures illustrate each occurrence of 51419.
TY - JOUR. T1 - Amplified fragment length polymorphism based identification of genetic markers and novel PCR assay for differentiation of Campylobacter fetus subspecies. AU - van Bergen, M.A.P.. AU - Simons, G.. AU - van der Graaf-van Bloois, L.. AU - van Putten, J.P.. AU - Rombout, J.. AU - Wesley, I.. AU - Wagenaar, J.A.. PY - 2005. Y1 - 2005. N2 - Differentiation of Campylobacter fetus into C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv) is important for both clinical and economic reasons. In the past, several molecular typing methods have been used for differentiation, including amplified fragment length polymorphism (AFLP). In this study, AFLP was employed to identify C. fetus subspecies specific markers that can serve as a basis for design of novel PCR primer sets for Cfv. Four groups of C. fetus strains with different phenotypic or genotypic traits were examined by AFLP using 22 different DdeI/MboI primer combinations. Specific AFLP fragments were deduced and sequenced ...
Using the multiplex PCR assay all isolates yielded the same results: species-specific amplicons were observed but no subspecies-specific amplicons. The VenSF and VenSR subspecies-specific primer set used in the assay targets a segment of the Cfv genome. The generation of an amplicon of approximately 182-bp is indicative that the test organism is Cfv. Campylobacter fetus subsp. fetus is identified by exclusion. Based on the PCR test results obtained, all the field isolates should be classified as Cff.. DISCUSSION. In veterinary diagnostic laboratories the culture and isolation of C. fetus is the traditional method for the diagnosis of bovine campylobacteriosis. The inherent limitations of this methodology prompted this investigation to identify and evaluate a more sensitive means of detecting C. fetus. Molecular techniques, particularly PCR, are becoming more practical and affordable for diagnostic laboratories to incorporate into their test portfolio. The specificity and sensitivity of assays, ...
The correlation between the molecular size of the surface layer protein (S protein) and both structure and antigenicity of the Campylobacter fetus surface layer (S layer) was investigated in several clinical strains and their spontaneous variants which produce S proteins of molecular weights (MW) different from those of the parents. Only three molecular sizes of the S proteins were observed (98, 127, and 149 kDa) in the parental and variant strains. Immunologically, the 98-kDa protein and the 149-kDa protein but not the 127-kDa protein were cross-reactive. Freeze-etching analysis showed that the 98-kDa S protein formed a hexagonal arrangement with a 24-nm center-to-center space and that the S proteins with larger MW (127 or 149 kDa) formed tetragonal ones with an 8-nm center-to-center space. Thus, the MW changes of the S proteins seen in the variant strains were associated with both morphological and antigenic changes in S layer. These observations support the hypothesis that the pattern and ...
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Campylobacter fetus is a Gram-negative bacterium that has caused several cases of human and animal disease. Here, we report the draft genome sequence of C. fetus MMM01, isolated from the blood of a 60-year-old patient with type II diabetes and chronic kidney disease. The sequence has a total length of 1,740,393 bp and an average G�C content of 33.1%. The availability of the draft genome sequence of C. fetus MMM01 isolated from a case of chronic kidney disease will contribute to a better understanding of the pathophysiological mechanisms of this organism.. ...
Lineage: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; Campylobacter fetus; Campylobacter fetus subsp. ...
Lineage: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Campylobacteraceae; Campylobacter; Campylobacter fetus; Campylobacter fetus subsp. ...
C. fetus had been described in association with abortion in cattle and sheep and as a cause of bovine infertility. The latter is a venereal disease, resulting in asymptomatic infection in the prepucial sac of the male. It was formerly widespread in the UK. Diagnosis relies on the isolation of the organism and the demonstration of agglutinins in vaginal mucus in females of an infected herd. Since 1974, thirty outbreaks of campylobacter infertility have been diagnosed in the South West of Scotland. Biochemical methods differentiate C. fetus subsp. fetus (formerly subsp. intestinalis) from two biotypes of subsp. venerealis. Up to the present it has been thought that all venereally transmitted strains of C. fetus subsp. fetus in Britain were serotype B. However, all isolates from cases of infertility in the South West of Scotland have proved to be sero-type A, and recently C. fetus subsp. fetus serotype A has been reported from Germany. It can be concluded that all biotypes can be considered ...
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2014) Draft Genome Sequences of Campylobacter fetus subsp. venerealis bv. venerealis Strain B6 and bv. intermedius Strain 642-21. Genome Announcements, 2 (5). e00943. Ouyang, S., Zhang, D., Han, J., Zhao, X., Cui, Y., Song, W., Huo, N., Liang, Y., Xie, J., Wang, Z., Wu, Q., Chen, Y-X, Lu, P., Zhang, D-Y, Wang, L., Sun, H., Yang, T., Keeble-Gagnère, G., Appels, R., Doležel, J., Ling, H-Q, Luo, M., Gu, Y., Sun, Q. and Liu, Z. ...
The microbial population of the praeputial sac was studied in a group of ten young bulls housed in one section of the rearing farm in the age period from 2 to 14 months when they were transferred to A. I. stations. As to currently occurring microflora, Proteus sp. and Pseudomonas aeruginosa were isolated most frequently. The remaining micro-organisms isolated during the study included E. coli, non-haemolytic and viridizing streptococci, Staphylococcus epidermidis, micrococci, aerobic sporulating bacteria, saprophytic corynebacteria snd gamma-negative non-fermenting rods. Campylobacter foetus subsp. foetus was not detected and the same applies to the other species of this genus (Campylobacter sp.). When the bullocks were transferred to the rearing farm, the mycoplasms were no longer isolated from the praeputial mucous membrane. Mycoplasms were determined in all the studied animals after six months stay in group. The isolated strains were identified as M. bovigenitalium by the epiimmunofluorescence
TY - JOUR. T1 - Campylobacter surface-layers (S-layers) and immune evasion.. AU - Thompson, Stuart A.. PY - 2002/12. Y1 - 2002/12. N2 - Many pathogenic bacteria have evolved mechanisms for evading host immune systems. One evasion mechanism is manifest by the surface layer (S-layer), a paracrystalline protein structure composed of S-layer proteins (SLPs). The S-layer, possessed by 2 Campylobacter species (C. fetus and C. rectus), is external to the bacterial outer membrane and can have multiple functions in immune avoidance. C. fetus is a pathogen of ungulates and immunocompromised humans, in whom it causes disseminated bloodstream disease. In C. fetus, the S-layer is required for dissemination and is involved in 2 mechanisms of evasion. First, the S-layer confers resistance to complement-mediated killing in non-immune serum by preventing the binding of complement factor C3b to the C. fetus cell surface. S-layer expressing C. fetus strains remain susceptible to complement-independent killing, ...
Vibriosis definition is - an infectious disease of sheep and cattle caused by a bacterium (Campylobacter fetus synonym Vibrio fetus) and marked by infertility and abortion; also : infection with or disease caused by a vibrio.
This is true. The sad part is that so many people do not believe it. We have been indoctrinated from the beginning to believe that vaccines and other things are good for us. Nobody does research for themselves, they just assume the government and doctors are perfect(God). Here is something to think about, the people who do get vaccines, do not want to be in contact with others who do not get them ie) schools, places of employment, etc. WHY??? If youre vaccinated, arent you protected from all the diseases? FOOLISH! People, please do your research! ...
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A survey of the isolation rate and population size of thermophilic campylobacters in lambs at slaughter was carried out to determine the seasonal variation of thermophilic campylobacters in ovine hosts. Isolation rates determined by enrichment methods were always higher than those using direct plating onto selective agar and showed that Campylobacter could be isolated from 91·7% (n = 360) of samples from the small intestine of the lambs. Enumerations (MPN), done monthly over a 2-year period, averaged 4·00 log 10 (n = 1080, S.D. 0·16) campylobacters g−1 fresh weight (fw) intestinal contents with some samples giving values higher than 7 log MPN gfw−1. These results show that the prevalence of thermophilic campylobacters in sheep intended for slaughter is much higher than previously reported. Statistical analyses showed that there was a significant seasonal periodicity in the Campylobacter populations in the small intestines of lambs at slaughter (P = 0·004) but that there was no ...
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the cytolethal distending toxin (cdt) gene clusters of campylobacter coli strain co1-243 and c. fetus strain col-187 were cloned and sequenced to understand the importance of cdt as a virulence factor. the cdt genes of c. coli and c. fetus consist of three closely linked genes termed cdta, cdtb, cdtc whose sizes are 774, 801, and 570 bp, and 702, 798, and 546 bp, respectively. the homologies of each subunit of cdt genes between c. jejuni and c. coli, c. jejuni and c. fetus, or c. coli and c. fet ...
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