S100 calcium-binding protein A9 (S100A9) also known as migration inhibitory factor-related protein 14 (MRP14) or calgranulin B is a protein that in humans is encoded by the S100A9 gene. The proteins S100A8 and S100A9 form a heterodimer called calprotectin. S100-A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase. MRP14 complexes with MRP-8 (S100A8), another member of the S100 family of calcium-modulated proteins; together, MRP8 and MRP14 regulate myeloid cell function by binding to Toll-like receptor 4 (TLR4) and the receptor for advanced glycation end products. Altered expression of the S100A9 protein is ...

In this work, we describe novel gadolinium containing designer nanoprobes displaying antibodies against Mrp-8/14 to target inflammation in a murine model of atherosclerosis. Molecular probes targeting atherosclerosis-associated moieties have been widely used in research settings.16-19 The challenge has been to identify suitable ligands that simultaneously provide sufficient selectivity and high levels of expression and serve in a pathophysiologic context so that ligation of the target results in neutral or even beneficial effects on the disease process. Inflammation-associated calgranulins, S100A8 (Mrp8) and S100A9 (Mrp14) are upregulated following activation in response to cell contact with activated endothelium.8,20,21 Mrp-14 forms a heterodimeric complex with Mrp-8 and is isolated almost exclusively in the dimeric form (Mrp).1,3-5 Mrp-14 is functionally homologous across species, is highly expressed in atherosclerosis, and participates in amplification of inflammation, providing a ...

The calcium-binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) and MRP-14 (S100A9) belong to the S100 protein family. The…

Clone REA917 recognizes the calcium- and zinc-binding protein S100A8, also known as migration inhibitory factor-related protein 8 (MRP-8), calprotectin, or calgranulin-A. S100A8 is mainly expressed in myeloid cells, e.g., monocytes, macrophages, and granulocytes and upregulated in different tumors, e.g., in skin, breast, colon, pancreatic, bladder, and ovarian malignancies. S100A8 is involved in the regulation of inflammatory processes and immune response and plays a role in antimicrobial, cytostatic, and chemotactic activities.Additional information: Clone REA917 displays negligible binding to Fc receptors. - Norge

CAGBMigration inhibitory factor-related protein 14; Calgranulin B; calgranulin-B; Calprotectin L1H subunit; CFAGMRP-14,60B8AG; CGLB; L1AG; LIAG; MAC387; MIF; MRP-14; MRP14Leukocyte L1 complex heavy chain; NIF; P14; protein S100-A9; S100 calcium binding protein A9 (calgranulin B); S100 calcium binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100 calcium-binding protein A9; S100A9 ...

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Using transcriptional profiling of platelets from patients presenting with acute myocardial infarction, we identified myeloid-related protein-14 (MRP-14, also known as S100A9) as an acute myocardial infarction gene and reported that platelet MRP-14 binding to platelet CD36 regulates arterial thrombosis. However, whether MRP-14 plays a role in venous thrombosis is unknown. We subjected WT and Mrp-14-deficient (Mrp-14-/-) mice to experimental models of deep vein thrombosis (DVT) by stasis ligation or partial flow restriction (stenosis) of the inferior vena cava. Thrombus weight in response to stasis ligation or stenosis was reduced significantly in Mrp-14-/- mice compared with WT mice. The adoptive transfer of WT neutrophils or platelets, or the infusion of recombinant MRP-8/14, into Mrp-14-/- mice rescued the venous thrombosis defect in Mrp-14-/- mice, indicating that neutrophil- and platelet-derived MRP-14 directly regulate venous thrombogenesis. Stimulation of neutrophils with MRP-14 induced ...

The p38 MAPK pathway participates in a number of neutrophil functions critical to generation and regulation of the inflammatory response, including chemotaxis, adherence, respiratory burst activity, degranulation, and cytoskeletal reorganization (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12). Understanding the molecular mechanisms by which p38 MAPK participates in these responses is hindered by the limited number of targets of p38 MAPK identified to date in neutrophils. MAPKAPK2 and p47phox are the only clearly identified p38 MAPK targets in human neutrophils (1, 9, 10, 21). A previous study by Lewis et al. (45) determined that 20 of 25 ERK targets identified in a global screen were not previously known. Thus, it is likely that a number of important targets of p38 MAPK that participate in regulation of neutrophil responses remain to be identified. The goal of the present study was to apply a recently developed proteomic approach that allows simultaneous identification of multiple substrates of a single ...

Oligosaccharides are increasingly being recognized as important mediators of signaling in innate and adaptive immune responses (59, 60, 61, 62, 63). Considerable diversity of oligosaccharide structures provides enormous potential for information display on cell surfaces and specific recognition by different lectins. Glycans that have the same structure can also have different functions depending upon the proteins and cell types that carry them. Examples are the selectin ligands that mediate both inflammation-initiated leukocyte rolling as well as physiological lymphocyte homing and recirculation. However, not all glycan structures in mammals have been proven, much less functionally characterized. We earlier identified a family of novel carboxylated glycans on endothelial cells and macrophages that mediate inflammation. Here, we show that interfering with the interaction between these glycans and their putative lectin partners using a monoclonal anti-glycan Ab prevents the pathogenic process in a ...

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BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.

A structurally diverse group of endogenous molecules that are multifunctional, having physiological functions inside the cell, but when released from dying cells or from cells under stress or certain immune cells, they function to activate INNATE IMMUNITY. Uncontrolled and excessive release of alarmins may contribute to INFLAMMATION; CARCINOGENESIS, and NEOPLASM METASTASIS. Alarmins are also critical for heart and nerve tissue homeostasis. . ...

Schnitzlers syndrome (SchS) is a disabling autoinflammatory disorder, characterized by a chronic urticarial rash, an M-protein, arthralgia, and other signs of systemic inflammation. Anti-interleukin-1 (IL-1) beta antibodies are highly effective, but the pathophysiology is still largely unknown. Here we studied the effect of in-vivo IL-1 inhibition on serum markers of inflammation and cellular immune responses. Eight patients with SchS received monthly subcutaneous (s.c.) injections with 150 mg canakinumab for six months. Blood was drawn for measurement of serum markers of inflammation (12 times per patient) and for functional and phenotypic analysis of both freshly isolated and toll-like receptor (TLR)-ligand-stimulated peripheral blood mononuclear cells (PBMCs) (five times per patient). All data were compared to results of healthy controls. IL-6 levels in serum and in lysates of freshly isolated PBMCs and serum myeloid-related protein (MRP8)/14 and S100A12 levels correlated with disease activity. In

From NCBI Gene:. This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]. From UniProt: ...

PAA114Mu01, Polyclonal Antibody to Placenta Growth Factor (PLGF), 胎盘生长因子(PLGF)多克隆抗体, PlGF2; PGF; PGFL; Placental Growth Factor-Like; Vascular Endothelial Growth Factor-Related Protein | 仅供体外研究使用，不用于临床诊断！请索取进口关税税单及报关单！

HDGFRP2 - HDGFRP2 (untagged)-Human hepatoma-derived growth factor-related protein 2 (HDGFRP2), transcript variant 2 available for purchase from OriGene - Your Gene Company.

In this study, we characterized mice that were deficient in the S100 protein MRP-14 (S100A9). Although there was normal expression of MRP-8 mRNA in the MRP-14−/− myeloid cells, surprisingly, no MRP-8 protein was present. The absence of MRP-8 protein could be due to inefficient translation of MPR-8 mRNA or, more likely, due to instability of MRP-8 protein in the absence of its partner, MRP-14. This finding contrasts with evidence that murine MRP-14 and MRP-8 (CP-10) exist separately in myeloid cells (28, 40) and that CP-10 alone can function as a potent chemotactic factor (28, 29). In addition, immunohistochemical studies of mouse tissues show that MRP-14 (this study) and MRP-8 (data not shown) are coexpressed in myeloid cells. In support of this finding, the heterodimer can be isolated from spleen (data not shown) and bone marrow (13). Information from physical studies with the human proteins indicates that MRP-8 and MRP-14 form a heterodimer more readily than either forms a homodimer and ...

BÜHLMANN is a world leader in calprotectin and offers a range of assays as diagnostic tools to determine the inflammatory disease process.

MRP8小鼠单克隆抗体[MRP8 7C12/4](ab20220)可与人样本反应并经WB, ELISA, IHC, Flow Cyt实验严格验证，被1篇文献引用。所有产品均提供质保服务，中国75%以上现货。

MRP4兔多克隆抗体(ab32550)可与大鼠, 人样本反应并经WB实验严格验证，被2篇文献引用。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。

in Clinical Chemistry (2008), 54. BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze ... [more ▼]. BACKGROUND: SELDI-TOF mass spectrometry (MS) is a high-throughput proteomic approach with potential for identifying novel forms of serum biomarkers of arthritis. METHODS: We used SELDI-TOF MS to analyze serum samples from patients with various forms of inflammatory arthritis. Several protein profiles were collected on different Bio-Rad Laboratories ProteinChip arrays (CM10 and IMAC-Cu(2+)) and were evaluated statistically to select potential biomarkers. RESULTS: SELDI-TOF MS analyses identified several calgranulin proteins [S100A8 (calgranulin A), S100A9 (calgranulin B), S100A9*, and S100A12 (calgranulin C)], serum amyloid A (SAA), SAA des-Arg (SAA-R), and SAA des-Arg/des-Ser (SAA-RS) as biomarkers and confirmed the results with other techniques, ...

Our previous studies illustrated that deletion of RAGE was protective in murine models of long-term diabetes-associated neuropathy (14,15). Because RAGE is critically involved in inflammatory mechanisms by virtue of its ability to bind members of the S100/calgranulin family, HMGB1 and Mac-1 (19,20,36), we tested its role in superimposed acute nerve crush injury. These studies bear clinical relevance because diabetic subjects often sustain thermal and other acute injuries to their extremities during advancing neuropathy (7-10). This present work reveals that RAGE, particularly in bone marrow cells and in diabetes, contributes to maladaptive inflammatory mechanisms after acute nerve crush.. Our data confirmed that diabetes was associated with increased expression of AGEs in the peripheral nerve in the basal state (15) and buttressed our findings that AGE levels were lower in diabetic RAGE-null mice compared with diabetic WT mice (37). We previously showed that RAGE suppresses mRNA and protein ...

Human S100A7 Induces Mature Interleukin1α Expression by RAGE-p38 MAPK-Calpain1 Pathway in Psoriasis. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

References for Abcams Recombinant Human S100 alpha 2 protein (ab104821). Please let us know if you have used this product in your publication

References for Abcams Recombinant Human S100A4 protein (ab85489). Please let us know if you have used this product in your publication

Ops I meant CRP Are these different measures and tests or are they the same thing? ETA: ok I realize now that they are different. My CRP is .7 (within...

BACKGROUND/OBJECTIVES: Obesity is related to a dynamic extracellular matrix (ECM) remodeling, which involves the synthesis and degradation of different proteins, such as tenascin C (TNC) in the adipose tissue (AT). Given the functional relationship between leptin and inducible nitric oxide synthase (iNOS), our aim was to analyze the impact of the absence of the iNOS gene in AT inflammation and ECM remodeling in ob/ob mice. SUBJECTS/METHODS: The expression of genes involved in inflammation and ECM remodeling was evaluated in 10-week-old male double knockout (DBKO) mice simultaneously lacking the ob and iNOS genes as well as in ob/ob mice classified into three groups [control, leptin-treated (1 mg kg -1 day -1 ) and pair-fed ...

Dive into the research topics of The neuronal pentraxin-2 pathway is an unrecognized target in human neuroblastoma, which also offers prognostic value in patients. Together they form a unique fingerprint. ...

COLLECTION OF FAECAL SAMPLES | Sampling of faecal calprotectin; general adviceIn proctitis the area of inflamed mucosa is very limited and the contact time…

Our results indicate a steady decline in calprotectin concentration in the first 6 days after stool collection in samples kept at room temperature. A similar slope was seen in stool extracts kept at room temperature, while the stability of calprotectin was preserved in samples stored in a refrigerator at 4°C. We did not test the superiority of one ELISA kit over another. For that, we would have needed to perform a multitude of tests. The decline in calprotectin concentration over time was observed regardless of the ELISA kit used, which makes inadvertently smoothening of the curve unlikely.. Literature on calprotectin stability under various preservation conditions is scarce. A Swedish group observed a decline in calprotectin when stool samples were stored at room temperature for 7 days but claimed that the concentration remained unchanged in the first 3 days after collection.4 A Spanish group claimed that calprotectin remained stable in stool extracts stored at room temperature for 4 days,5 as ...

0083] 1. Davies J R, Rudd J H, Weissberg P L. Molecular and metabolic imaging of atherosclerosis. J Nucl Med. 2004; 45:1898-1907. [0084] 2. Kislinger T, Fu C, Huber B, Qu W, Taguchi A, Yan S D, Hofmann M, Yan S F, Pischetsrieder M, Stern D, Schmidt A M. N.sup.ε-(carboxymethyl) lysine adducts of proteins are ligands for receptor for advanced glycation endproducts that activate cell signaling pathways and modulate gene expression. J Biol Chem. 1999; 274:31740-31749. [0085] 3. Hofmann M A, Drury S, Fu C, Qu W, Taguchi A, Lu Y, Avila C, Kambham N, Bierhaus A, Nawroth P, Neurath M F, Slattery T, Beach D, McClary J, Nagashima M, Morser J, Stern D, Schmidt A M. RAGE mediates a novel proinflammatory axis: a central cell surface receptor for S100/calgranulin polypeptides. Cell. 1999; 97:889-901. [0086] 4. Schmidt A M, Yan S D, Brett J, Mora R, Nowygrod R, Stern D. Regulation of human mononuclear phagocyte migration by cell surface binding proteins for AGE. J. Clin. Invest. 1993; 91:2155-2168. [0087] 5. ...

0175]1. Hugel B, Martinez M C, Kunzelmann C, Freyssinet J M. Membrane microparticles: two sides of the coin. Physiology (Bethesda, Md. February 2005; 20:22-27. [0176]2. Mallat Z, Benamer H, Hugel B, Benessiano J, Steg P G, Freyssinet J M, Tedgui A. Elevated levels of shed membrane microparticles with procoagulant potential in the peripheral circulating blood of patients with acute coronary syndromes. Circulation. Feb. 29 2000; 101(8):841-843. [0177]3. Nieuwland R, Berckmans R J, Rotteveel-Eijkman R C, Maquelin K N, Roozendaal K J, Jansen P G, ten Have K, Eijsman L, Hack C E, Sturk A. Cell-derived microparticles generated in patients during cardiopulmonary bypass are highly procoagulant. Circulation. Nov. 18 1997; 96(10):3534-3541. [0178]4. Tesse A, Martinez M C, Hugel B, Chalupsky K, Muller C D, Meziani F, Mitolo-Chieppa D, Freyssinet J M, Andriantsitohaina R. Upregulation of proinflammatory proteins through NF-kappaB pathway by shed membrane microparticles results in vascular hyporeactivity. ...

Calprotectin is released by white blood cells (neutrophils) in the digestive tract with inflammation. Calprotectin tests measure levels in stool to help detect conditions such as inflammatory bowel disease (IBD) and infections.

Semantic Scholar extracted view of Dynamic changes in calprotectin and its correlation with traditional markers of oxidative stress in patients with acute ischemic stroke. by Antonios Chatzopoulos et al.

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When asking the question of carboxylated vs. non-carboxylated, the answer comes down to an adhesion coating controlled by carboxylation.

Kit Component:- KN203070G1, MRPS18A gRNA vector 1 in pCas-Guide vector- KN203070G2, MRPS18A gRNA vector 2 in pCas-Guide vector- KN203070D, donor…

複合型酸化的リン酸化異常(COXPD)は、ミトコンドリアの酸化的リン酸化システムの欠陥により起こる多様な症状を持つ疾患群である。リボソームタンパクの遺伝子(MRPS16 and MRPS22)の変異は、出生前に重症な小児病を引き起こすことが報告されている。また、COXPD患者のミトコンドリアの翻訳伸長因子の遺伝子(GFM1, TUFM, TSFM およびC12orf65)に変異があることも報告されている ...

Objective: To study the active involvement of S100A8 and A9 in joint inflammation and cartilage destruction during antigen-induced arthritis (AIA).. Methods: Joint inflammation and cartilage destruction was measured with 99mTc uptake and histology. The role of S100A8/A9 was investigated by inducing AIA in S100A9-/- mice, which lacks also S100A8 at protein level, or after intra-articular injection of rS100A8 in mouse kneejoints. Cartilage destruction was measured using immunolocalisation of the neoepitope VDIPEN or NITEGE. mRNA levels of MMPs and cytokines were measured using RT-PCR.. Results: Immunization of S100A9-/- mice with the antigen mBSA induced normal cellular and humoral responses, not different from WT controls. However, joint swelling measured at day 3 and 7 after AIA induction was significantly lower (36 and 70% respectively). Histologically, at day 7 AIA, cellular mass was much lower (63- 80%) and proteoglycan depletion from cartilage layers was significantly reduced (between ...

One of the most definitive examples of a vertebrate extraorganismal structural protein can be found in three-spined sticklebacks (Gasterosteus aculeatus). In the breeding male the kidney hypertrophies and synthesizes an adhesive protein called spiggin, which is secreted into the urinary bladder from where it is employed as a structural thread for nest building. This paper describes the first molecular characterization of spiggin and demonstrates that this adhesive is a protein complex assembled from a potential of three distinct subunits (alpha, beta, and gamma). These subunits arise by alternative splicing, and 11-ketoandrogens induce their expression in stickleback kidneys. Analysis of the predicted amino acid sequence of each subunit reveals a modular organization whose structural elements display a similarity to the multimerization domains found within von Willebrand Factor-related proteins. These results implicate that spiggin utilizes a conserved multimerization mechanism for the ...

Inflammatory mediators trigger polymorphonuclear neutrophils (PMN) to produce reactive oxygen species (ROS: O2-, H2O2, ∙OH). Mediated by myeloperoxidase in PMN, HOCl is formed, detectable in a chemiluminescence (CL) assay. We have shown that the abundant cytosolic PMN protein calprotectin (S100A8/A9) similarly elicits CL in response to H2O2 in a cell-free system. Myeloperoxidase and calprotectin worked synergistically. Calprotectin-induced CL increased, whereas myeloperoxidase-triggered CL decreased with pH | 7.5. Myeloperoxidase needed NaCl for CL, calprotectin did not. 4-hydroxybenzoic acid, binding ∙OH, almost abrogated calprotectin CL, but moderately increased myeloperoxidase activity. The combination of native calprotectin, or recombinant S100A8/A9 proteins, with NaOCl markedly enhanced CL. NaOCl may be the synergistic link between myeloperoxidase and calprotectin. Surprisingly- and unexplained- at higher concentration of S100A9 the stimulation vanished, suggesting a switch from pro-oxidant to

Division of Chemical Biology and Medicinal Chemistry. Researching the COVID-19 spike protein that creates infection in a host by binding to its cell receptors. Recent scientific reporting suggests heparin sulfate interacts with the COVID-19 spike protein, and the Liu group seeks to determine which specific heparin sulfate structure displays the highest affinity to COVID-19 spike protein. Heparin sulfate is also known to attenuate inflammation induced by a COVID-19 infection - a significant outcome in many infected patients that causes uncontrolled inflammation responses in the lung, leading to lung failure. Using heparin sulfate, Lius team will seek to inhibit a series of proinflammatory proteins released after COVID-19 infection to reduce symptoms in patients.. Learn more about the Jian Liu Lab ...

Head and neck squamous cell carcinoma express high levels of the EF-hand calcium-binding protein S100A2 in contrast to other tumorigenic tissues and cell lines where the expression of this protein is reduced. Subtractive hybridization of tumorigenic versus normal tumor-derived mammary epithelial cells has previously identified the S100A2 protein as potential tumor suppressor. The biological function of S100A2 in carcinogenesis, however, has not been elucidated to date. Here, we report for the first time that during recovery from hydroxyurea treatment, the S100A2 protein translocated from the cytoplasm to the nucleus and co-localized with the tumor suppressor p53 in two different oral carcinoma cells (FADU and SCC-25). Co-immunoprecipitation experiments and electrophoretic mobility shift assay showed that the interaction between S100A2 and p53 is Ca(2+)-dependent. Preliminary characterization of this interaction indicated that the region in p53 involved with binding to S100A2 is located at the C ...

Results qPCR analysis showed increased expression of the alarmins S100A8/A9 and several members of the canonical Wnt signaling pathway in the CIOA model at all time points measured. In the DMM model, the expression of S100A8/A9 and Wnt16 and WISP1 was mainly increased at day 28 after induction. Kinetics of S100 and Wnt expression were comparable, as was observed in both models. This gave rise to the question if an interrelationship existed between these factors. Therefore, we overexpressed Wnt8a and Wnt16, two canonical Wnts, with the use of adenoviral vectors. However, this did not result in increased expression of S100A8 and S100A9, both on RNA and protein level. In contrast, we found that injection of S100A8 increased the expression of Wnt16 in the synovium and accumulation of β-catenin, a hallmark of canonical Wnt signaling, in both cartilage and synovium. In addition, the downstream mediator of canonical Wnt signaling WISP1, was increased. Furthermore, we found reduced β-catenin ...

Calprotectin (CP) is an antimicrobial protein produced and released by neutrophils that inhibits the growth of pathogenic microorganisms by sequestering essential metal nutrients in the extracellular space. In this work, spectroscopic and thermodynamic metal-binding studies are presented to delineate the zinc-binding properties of CP. Unique optical absorption and EPR spectroscopic signatures for the interfacial His3Asp and His4 sites of human calprotectin are identified by using Co(II) as a spectroscopic probe. Zinc competition titrations employing chromophoric Zn(II) indicators provide a 2:1 Zn(II):CP stoichiometry, confirm that the His[subscript 3]Asp and His[subscript 4] sites of CP coordinate Zn(II), and reveal that the Zn(II) affinity of both sites is calcium-dependent. The calcium-insensitive Zn(II) competitor ZP4 affords dissociation constants of K[subscript d1] = 133 ± 58 pM and K[subscript d2] = 185 ± 219 nM for CP in the absence of Ca(II). These values decrease to K[subscript d1] ...

Calprotectin is a 36kDa calcium and zinc binding protein expressed by the gene S100 calcium-binding protein A8, S100A8. It accounts for 30 to 40% of neutrophils cytosol. In vitro studies show it has bacteriostatic and fungistatic properties. It is...

Recombinant human S100B Calcium-Binding Protein, Western Blot Control protein - 230-00002-WBC. Liquid . Expression system is Escherichia coli (E.coli).