The amount of cell-surface Epidermal Growth Factor Receptor (EGFR) available to secreted ligand (EGF) dictates a cells ability to mediate cell proliferation, differentiation or migration. Multiple factors regulate EGFR cell-surface expression including the rates of protein synthesis and protein deg …

Human granulocytes are characterized by a variety of specific effector functions involved in host defense. Several widely expressed protein kinases have been implicated in the regulation of these effector functions. A polymerase chain reaction- based strategy was used to identify novel granulocyte-specific kinases.Anovel protein kinase complementary DNA with an open reading frame of 357 amino acids was identified with homology ... read more to calciumcalmodulin- dependent kinase I (CaMKI). This has been termed CaMKI-like kinase (CKLiK). Analysis of CKLiK messenger RNA (mRNA) expression in hematopoietic cells demonstrated an almost exclusive expression in human polymorphonuclear leukocytes (PMN). Up-regulation of CKLiK mRNA occurs during neutrophilic differentiation of CD341 stem cells. CKLiK kinase activity was dependent on Ca11 and calmodulin as analyzed by in vitro phosphorylation of cyclic adenosine monophosphate responsive element modulator (CREM). Furthermore, CKLiKtransfected cells treated ...

1MXE: Structure of the Complex of Calmodulin with the Target Sequence of Calmodulin-Dependent Protein Kinase I: Studies of the Kinase Activation Mechanism

Involvement of PCNK in CaMKIα phosphorylation.MCF-7 cells were transfected with siRNA targeting PNCK or control siRNA (NT1) and 72 h later, cells were exposed

We have professional and advanced research and production capacity for CAMKI reagents production, including Proteins, Antibodies, cDNA Clones,etc. All CAMK1 products are produced in house and quality controlled.

4316 An exciting recent advancement in EGFR biology is the discovery of EGFR mutations in the kinase domain that confer sensitivity to EGFR tyrosine kinase inhibitors and increased ligand (EGF)-induced EGFR phosphorylation. An important question is the mechanism by which these EGFR mutants have intrinsic kinase activity. For this study, we utilized a non-small cell lung cancer (NSCLC) cell line HCC827 with deletion of aa 746-750 in the kinase domain of EGFR. In the presence of 10% serum as well as after serum-deprivation for 2 days, HCC827 had increased levels of phosphorylated EGFR (p-EGFR) at the autophosphorylation site Y1068 as compared to six other cell lines with wild type EGFR. This suggests that HCC827 has ligand-independent EGFR kinase activity. This was also supported by the observation that treatment with GM6001, an inhibitor of matrix metalloproteinases that release EGFR ligands, or with the EGFR antibody C225, did not detectably affect basal p-EGFR in HCC827. In a panel of cell ...

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in a range of conditions and pathologies from prostate to hepatic cancer. Here, we describe the expression in Escherichia coli and the purification protocol for the following constructs: full-length CaMKK2 in complex with CaM, CaMKK2 apo, CaMKK2 (165-501) in complex with CaM, and the CaMKK2 F267G mutant. The protocols described have been optimized for maximum yield and purity with minimal purification steps required and the proteins subsequently used to develop a fluorescence-based assay for drug binding to the kinase, Using the fluorescent properties of STO-609 as a tool to assist structure-function analyses of recombinant CaMKK2 [1].

An influx of calcium into neurons causes mitochondrial fission, according to Han et al.. To respond to changing cellular needs, mitochondria move, fuse together, or undergo fission. Han et al. now reveal some of the proteins and signals that regulate fission in neurons.. The authors tested mitochondrial responses to an increase in potassium (K+) levels, which mimics an action potential. The K+ spike brought mitochondria to a halt and prompted their division, making them shorter and rounder. These changes depended on the opening of voltage-dependent calcium channels and the resulting Ca2+ gradient, which in turn activates the Ca2+/calmodulin-dependent protein kinase (CaMKIα). A CaMKIα antagonist blocked K+-triggered changes in mitochondrial shape.. In their search for CaMKIα substrates, the authors turned to Drp1 (dynamin-related protein 1), which promotes mitochondrial fission and whose sequence suggested it could be phosphorylated. And the authors found that it was, in fact, a CaMKIα ...

Buy our Recombinant mouse PNCK protein. Ab60891 is an active full length protein produced in Baculovirus infected Sf9 cells and has been validated in FuncS…

克拉玛尔专业提供CaMKII Phospho (pT286) (CAMK2A)高品质试剂,可查询CaMKII Phospho (pT286) (CAMK2A)价格,提供CaMKII Phospho (pT286) (CAMK2A)技术资料,CaMKII Phospho (pT286) (CAMK2A)定制合成和大包装,是专业生产各种高端试剂的制造商和供应商.联系电话4001650900

Plasmid pDONR223-CAMK1G from Dr. William Hahns lab contains the insert CAMK1G and is published in Nature. 2010 Nov 24. ():. This plasmid is available through Addgene.

Plasmid pWZL Neo Myr Flag CAMK4 from Dr. Jean Zhaos lab contains the insert CAMK4 and is published in Cell. 2007 Jun 15. 129(6):1065-1079. This plasmid is available through Addgene.

Calcium/calmodulin-dependent kinase kinase 2 (CaMKK2) has been implicated in the regulation of metabolic activity in cancer and immune cells, and affects whole-body metabolism by regulating ghrelin-signalling in the hypothalamus. This has led to efforts to develop specific CaMKK2 inhibitors, and STO-609 is the standardly used CaMKK2 inhibitor to date. We have developed a novel fluorescence-based assay by exploiting the intrinsic fluorescence properties of STO-609. Here, we report an in vitro binding constant of KD ∼17 nM between STO-609 and purified CaMKK2 or CaMKK2:Calmodulin complex. Whereas high concentrations of ATP were able to displace STO-609 from the kinase, GTP was unable to achieve this confirming the specificity of this association. Recent structural studies on the kinase domain of CaMKK2 had implicated a number of amino acids involved in the binding of STO-609. Our fluorescent assay enabled us to confirm that Phe(267) is critically important for this association since mutation of this

CaMK2 (calcium/calmodulin-dependent protein kinase 2), a multifunctional serine/threonine-protein kinase involved in diverse cellular processes, is vital for the transduction of the Ca2+ signaling cascade. Recently, research has highlighted the involvement of CaMK2 in cancer development. However, the specific effects of CaMK2 on cancer have not been fully elucidated. In this review, we summarize not only the altered expression of CaMK2 in a range of cancers, as evidenced by bioinformatics analysis, but also the significant role of CaMK2 in regulating cancer progression, such as proliferation and metastasis. In addition, we described the functional influence of CaMK2 on cancer stemness and resistance. Understanding the critical effects and mechanisms of CaMK2 in cancer would facilitate the development of a promising therapeutic strategy for cancer treatment.

TABLE-US-00006 TABLE 6 THI + 2 + 4 + 5 + 7 Composite (Meta-THc) OG- OG- OG- OG- OG- 3116 @ 3116 @ 3116 @ 3116 @ 3116 @ 50 100 1 μg/ml 5 μg/ml 25 μg/ml μg/ml μg/ml Abl(H396P)(h) 91 88 73 55 50 Abl(M351T)(h) 100 87 62 50 38 Abl(Q252H)(h) 89 86 58 45 44 Abl(h) 98 85 65 41 49 Abl(m) 99 87 60 47 43 Abl(T315I)(h) 100 91 79 65 52 Abl(Y253F)(h) 93 90 75 54 51 ACK1(h) 122 112 97 102 82 ALK(h) 76 38 17 16 26 ALK4(h) 96 95 85 68 48 Arg(h) 94 91 68 52 42 Arg(m) 100 99 94 73 50 ARK5(h) 100 97 82 64 75 Aurora-A(h) 92 79 40 41 27 Axl(h) 97 99 83 77 60 Blk(m) 95 102 71 49 54 Bmx(h) 91 94 84 77 43 BrSK1(h) 95 90 71 47 51 BrSK2(h) 91 82 77 70 63 BTK(h) 99 97 64 44 28 CaMKI(h) 95 84 46 28 48 CaMKII(r) 97 106 89 69 63 CaMKIIβ(h) 94 99 85 52 34 CaMKIIγ(h) 107 103 94 92 134 CaMKIIδ(h) 103 97 84 83 84 CaMKIV(h) 107 108 95 75 58 CaMKIδ(h) 91 93 92 75 80 CDK1/cyclinB(h) 99 101 91 71 58 CDK2/cyclinA(h) 105 106 92 83 63 CDK2/cyclinE(h) 99 103 75 60 42 CDK3/cyclinE(h) 108 100 96 79 45 CDK5/p25(h) 102 89 84 77 72 ...

THADA antibody [C3], C-term (thyroid adenoma associated) for WB. Anti-THADA pAb (GTX104909) is tested in Human samples. 100% Ab-Assurance.

Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.

View Tg(Camk2a-tTA)1Mmay/0 Tg(tetO-APPSwInd)102Dbo/0 involves: C3H/HeJ * C57BL/6 * CBA: phenotypes, images, diseases, and references.

Looking for online definition of CaM-kinase kinase 1 in the Medical Dictionary? CaM-kinase kinase 1 explanation free. What is CaM-kinase kinase 1? Meaning of CaM-kinase kinase 1 medical term. What does CaM-kinase kinase 1 mean?

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. In vitro phosphorylates CREB1 and SYN1/synapsin I. Phosphorylates and activates CAMK1 (By similarity).

Integrin signalling co-ordinates with signalling originating from growth factor receptors in the co-operative control of cell proliferation, survival and migration. Increasing evidence suggests that integrins form physical complexes at the cell membrane with growth factor receptors, giving rise to signalling platforms at the adhesive sites. It is probable that at these sites integrins regulate adhesion and at the same time physically constrain and direct the response to soluble growth factors towards proliferation or survival stimuli. These co-operative effects might depend on integrin ability to activate growth factor receptors. In the present paper, we summarize our recent study showing that integrin-dependent adhesion triggers ligand-independent EGFR (epidermal growth factor receptor) activation to transduce downstream signalling. In addition, we also show that integrin-induced signalling pathways are necessary for EGF-dependent transcriptional response, demonstrating the requirement of the ...

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

calmodulin-dependent protein kinase V: widely distributed in various tissues, involved in calcium-regulated processes; from rat brain; may exist in 40 & 41 kDa isoforms; amino acid sequence has been determined

Camk1g - Camk1g (untagged ORF) - Rat calcium/calmodulin-dependent protein kinase IG (Camk1g), (10 ug) available for purchase from OriGene - Your Gene Company.

Camk2d2 antibody (calcium/calmodulin-dependent protein kinase (CaM kinase) II delta 2) for IHC-P, WB. Anti-Camk2d2 pAb (GTX124377) is tested in Zebrafish samples. 100% Ab-Assurance.

Expression of CAMK4 (CaMK-GR) in duodenum tissue. Antibody staining with HPA011753, HPA017206 and CAB004347 in immunohistochemistry.

2IZV: Structure of the Socs4-Elonginb/C Complex Reveals a Distinct Socs Box Interface and the Molecular Basis for Socs-Dependent Egfr Degradation.

We demonstrate for the first time that 4H-1,2,6-thiadiazin-4-one (TDZ) can function as a chemotype for the design of ATP-competitive kinase inhibitors. Using insights from a co-crystal structure of a 3,5-bis(arylamino)-4H-1,2,6-thiadiazin-4-one bound to calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2), several analogues were identified with micromolar activity through targeted displacement of bound water molecules in the active site. Since the TDZ analogues showed reduced promiscuity compared to their 2,4-dianilinopyrimidine counter parts, they represent starting points for development of highly selective kinase inhibitors.

Background: CaMKK β is a major kinase activated by elevated levels of intracellular calcium. Our previous data has suggested that CaMKK β is neuroprotective after stroke in young mice as inhibition of this kinase aggravated stroke outcome. As aging is an important determinant of stroke outcomes, here we evaluated the functional role of CaMKK β in stroke in aged mice.. Methods: Aged wild type (WT) males received intracerebral injections of lentiviral vectors carrying either CaMKK β (LV-CaMKK β) or GFP (LV-GFP) 7 days before middle cerebral artery occlusion (MCAO, 90 minutes). Acute infarcts and neurological deficits scores were then analyzed at 72 hours post MCAO. In chronic survival studies, aged male CaMKK β knockout (KO) and WT mice were subjected to 60 minutes MCAO. Following stroke, long-term behavioral assessments were continuously performed for 3 weeks in KO and WT mice until the sacrifice for tissue loss assessments.. Results: Baseline levels of CaMKK β in aged brain were ...

Recombinant Calcium/calmodulin-Dependent Protein Kinase IV (CAMK4) Protein (His tag). Spezies: Human. Quelle: Escherichia coli (E. coli). Jetzt Produkt ABIN668012 bestellen.

The IUPHAR/BPS Guide to Pharmacology. calcium/calmodulin-dependent protein kinase II gamma subunit - CAMK2 family. Detailed annotation on the structure, function, physiology, pharmacology and clinical relevance of drug targets.

TY - JOUR. T1 - Amphetamine activate protein kinase C and calcium/calmodulin-dependent protein kinase via NMDA receptor in primary cultures of rat cortical neurons. AU - Wu, Hsueh-Hsia. AU - Lee, Horng-Mo. PY - 1999. Y1 - 1999. M3 - Article. VL - 1. SP - 12. EP - 19. JO - New Taipei Journal of Medicine. JF - New Taipei Journal of Medicine. SN - 1562-4242. ER - ...

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Expression of CAMK2A (CAMKA, CaMKIINalpha, KIAA0968) in ovary tissue. Antibody staining with HPA051783, HPA051785, HPA053973 and CAB004330 in immunohistochemistry.

This page contains the article Correctly Completing a Medicare Claim http://www.chiro.org/Medicare/ABSTRACTS/Correctly_Completing_a_Medicare_Claim.shtml

Olomoucine is known as a cyclin-dependent kinase inhibitor. We found that olomoucine blocked insulins ability to stimulate glucose transport. It did so without affecting the activity of known insulin signaling proteins. To identify the olomoucine-sensitive kinase(s), we prepared analogs that could be immobilized to an affinity resin to isolate binding proteins. One of the generated analogs inhibited insulin-stimulated glucose uptake with increased sensitivity compared with olomoucine. The IC50 for inhibition of insulin-stimulated glucose uptake occurred at analog concentrations as low as 0.1 μm. To identify proteins binding to the analog, [35S]-labeled cell lysates prepared from 3T3-L1 adipocytes were incubated with analog chemically cross-linked to a resin support and binding proteins analyzed by SDS-PAGE. The major binding species was a doublet at 50-60 kDa, which was identified as calcium/calmodulin-dependent protein kinase II (CaMKII) by N-terminal peptide analysis and confirmed by ...

Camkk1 - Camkk1 (Myc-DDK-tagged ORF) - Rat calcium/calmodulin-dependent protein kinase kinase 1, alpha (Camkk1), (10 ug) available for purchase from OriGene - Your Gene Company.

Neonatal hypoxia-ischemia (HI) is a major cause of death and disability in neonates. HI leads to a dramatic rise in intracellular calcium levels, which was originally thought to be detrimental to the brain. However, it has been increasingly recognized that this calcium signaling may also play an important protective role after injury by triggering endogenous neuroprotective pathways. Calcium/calmodulin-dependent protein kinase kinase ß (CaMKK ß) is a major kinase activated by elevated levels of intracellular calcium. Here we evaluated the functional role of CaMKK ß in neonatal mice after HI in both acute and chronic survival experiments. Postnatal day ten wild-type (WT) and CaMKK ß knockout (KO) mouse male pups were subjected to unilateral carotid artery ligation, followed by 40 min of hypoxia (10% O2 in N2). STO-609, a CaMKK inhibitor, was administered intraperitoneally to WT mice at 5 minutes after HI. TTC (2,3,5-triphenyltetrazolium chloride monohydrate) staining was used to assess ...

Results We show that culture of MRL/lpr Foxp3-GFP T cells in the presence of KN-93 promotes Treg differentiation in a dose dependent manner (Fig. F). Treatment of MRL/lpr Foxp3-GFP mice with KN-93 results in significant induction of Treg cells in the spleen, peripheral lymph nodes (Fig. B-E) and peripheral blood (Fig. A and B) and this is accompanied by decreased skin and kidney damage. Notably, KN-93 clearly diminishes the accumulation of inflammatory cells along with reciprocally increased Treg cells in target organ.. ...

Overview Protocols Specifications Resources Convenient, 1-Hour Kinase Activity Assays The SignaTECT® Protein Kinase Assay Systems provide a rapid, radioactivity-based method for detection of kinase activity. The method is based on capture of phosphorylated labeled substrates on the SAM2® Biotin Capture Membrane, which

AMPK is a ubiquitously expressed heterotrimeric kinase that plays a key role in cellular energy homeostasis (2). AMPK consists of an α, a β, and a γ subunit and is activated by the upstream kinases such as calcium calmodulin-dependent protein kinase kinase (CaMKK) and serine/threonine kinase 11 (LKB1) via phosphorylation of threonine residue 172 (47). Activation of AMPK by CaMKK and LKB1 is dependent, respectively, on intracellular calcium and the AMP:ATP ratio (47). During energy-depleted conditions, intracellular concentrations of AMP rise while ATP levels fall, leading to increased activation of AMPK and phosphorylation of its multiple substrates to enhance catabolism and suppress anabolic energy consumption. In excess energy states, reduced AMPK activation stimulates protein synthesis, cell growth, and storage. AMPK activity is also independently regulated by circulating hormones and cytokines, including bradykinin and the adipokines leptin and adiponectin (2,47). AMPK is also induced by ...

Supplementary MaterialsAdditional document 1: Body S1. for profiling little RNAs. Body S15. The saturation curves of miRNA. Body S16. RPM scatterplots of portrayed small RNAs. Body S17. Comparative expression heat maps of super-enhancer-regulated professional mRNAs and miRNAs. Body S18. Hematoxylin and Eosin (HE) staining from the HCC tissues. Figure S19. Comparative expression degrees of gene groupings between HCC Exp-subpopulations. Body S20. mRNA catch sequencing from the Holo-Seq total RNA collection. Body S21. mRNA and miRNA single transcriptome analyses of hepatocellular carcinoma (HCC) one cells. (DOCX 5908 kb) 13059_2018_1553_MOESM1_ESM.docx (5.7M) GUID:?8BF5D1B7-5F74-410D-8E95-CCE7DDE5D5D7 Extra file YM348 2: Desk S1. Not really1-site-containing transcripts in mouse. Desk S2. Not really1-site-containing transcripts in individual. Desk S3. Sequencing figures of RNA libraries. Desk S4. One cell collection price with different strategies. (XLSX 171 kb) 13059_2018_1553_MOESM2_ESM.xlsx ...

Human CAMK2D full-length ORF ( NP_742126.1, 1 a.a. - 478 a.a.) recombinant protein with GST-tag at N-terminal. (H00000817-P01) - Products - Abnova

If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...

Contact Us. Tel:732-484-9848. Fax:888-484-5008. Email:[email protected]. Add:1 Deer Park Dr, Suite Q,. Monmouth Junction, NJ 08852, USA. ...

Raval A, Tanner SM, Byrd JC, Angerman EB, Perko JD, Chen SS, Hackanson B, Grever MR, Lucas DM, Matkovic JJ, Lin TS, Kipps TJ, Murray F, Weisenburger D, Sanger W, Lynch J, Watson P, Jansen M, Yoshinaga Y, Rosenquist R, de Jong PJ, Coggill P, Beck S, Lynch H, de la Chapelle A, Plass C. Downregulation of death-associated protein kinase 1 (DAPK1) in chronic lymphocytic leukemia. Cell. 2007 Jun 01; 129(5):879-90 ...

The abundantly expressed calcium/calmodulin-dependent protein kinase II (CAMK2), alpha (CAMK2A), and beta (CAMK2B) isoforms are essential for learning and memory formation. Recently, a de novo candidate mutation (p.Arg292Pro) in the gamma isoform of CAMK2 (CAMK2G) was identified in a patient with severe intellectual disability (ID), but the mechanism(s) by which this mutation causes ID is unknown. Here, we identified a second, unrelated individual, with a de novo CAMK2G p.Arg292Pro mutation, and used in vivo and in vitro assays to assess the impact of this mutation on CAMK2G and neuronal function. We found that knockdown of CAMK2G results in inappropriate precocious neuronal maturation. We further found that the CAMK2G p.Arg292Pro mutation acts as a highly pathogenic gain-of-function mutation, leading to increased phosphotransferase activity and impaired neuronal maturation as well as impaired targeting of the nuclear CAMK2G isoform. Silencing the catalytic site of the CAMK2G p.Arg292Pro protein ...

The graphic displays domains and Protease cut sites on the protein sequence. Drag your mouse right/left over the graphic. Use the selection boxes on the right to select which annotations to view simultaneously. Combine annotation with multiple checkmarks.. ...

Radial glial cell-neuron interaction directs axon formation at the opposite side of the neuron from the contact siteRadial glial cell-neuron interaction directs axon formation at the opposite side of the neuron from the contact site ...

Recombinant full-length human CAMK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CAMK4 is a multifunctional serine/threonine protein kinase and a member of Ca2+/calmodulin-dependent protein kinase family.

STO-609 acetate,7-Oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylicacidacetate 52029-86-4 NMR spectrum, STO-609 acetate,7-Oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylicacidacetate H-NMR spectral analysis, STO-609 acetate,7-Oxo-7H-benzimidazo[2,1-a]benz[de]isoquinoline-3-carboxylicacidacetate C-NMR spectral analysis ect.