These β-TrCP2|sup|f|/sup| β-TrCP1|sup|-|/sup| mice harbor two mutations; the β-TrCP2 floxed allele (|i|Fbxw11|sup|tm1.1Ybn|/sup||/i|) and the β-TrCP1 knockout allele (|i|Btrc|sup|tm1Paga|/sup||/i|). These mice are a Cre recombinase-inducible double knockout of β-TrCP function and allow study of multiple cellular processes (Wnt pathway, NF-κB pathway, cell cycle regulation, DNA damage response) and cancer.

Khaw, L.E., Bohm, G.A., Metcalfe, S., Staunton, J. and Leadlay, P.F. (1998). „Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase. J. Bacteriol. 180: 809-814. PMID 9473033 ...

TY - JOUR. T1 - Crystal structure of β-galactosidase from Bacillus circulans ATCC 31382 (BgaD) and the construction of the thermophilic mutants （共著）. AU - Nakabayashi, Makoto. PY - 2015. Y1 - 2015. M3 - Article. JO - FEBS J. 2015 Jul;282(13):2540-52.. JF - FEBS J. 2015 Jul;282(13):2540-52.. ER - ...

Neste trabalho foi desenvolvido um novo bioprocesso para a síntese de lactosacarose, um candidato a prebiótico. A lactosacarose foi produzida por transgalactosilação, catalisada pela β-galactosidase de Bacillus circulans imobilizada em macroesfera de quitosana, utilizando a lactose e a sacarose como substratos. No processo de imobilização, os resultados indicam que a melhor razão entre a concentração de enzima e de suporte foi de 200 mg.g-1. A estabilidade térmica da enzima imobilizada foi determinada e comparada com a estabilidade térmica da enzima livre em temperaturas de 50, 60 e 70 °C e para esta última foi verificada a estabilidade na presença e ausência de substrato. A imobilização aumentou de 10 a 260 vezes a estabilidade térmica da enzima, sendo este efeito inversamente relacionado com a temperatura. A otimização das condições de produção indica, para a β-galactosidase imobilizada e livre, que a melhor condição de produção de lactosacarose e de ...

IMMOBILIZATION AND PROPERTIES OF BACILLUS CIRCULANS LEVANSUCRASE HEBA A EL-REFAİ, AHMED F ABDEL-FATTAH, FATEN A MOSTAFA Abstract PDF Similar Articles ...

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Also acts on the antibiotics neomycin, paromomycin, neamine, paromamine, vistamycin and gentamicin A. An enzyme from Pseudomonas aeruginosa also acts on butirosin ...

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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

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Deoxyribonucleic acid reassociation measurements revealed that 50 of 111 Bacillus circulans Jordan 1890 strains examined could be separated into 10 groups of genetically related organisms. The results of deoxyribonucleic acid relatedness studies and phenotypic comparisons suggested that five of these groups were hitherto undescribed species and that four others probably represented previous ly, but inadequately, described species. The tenth group, which included the type strain, represented the species B. circulans sensu stricto. Our data show that B. circulans and Bacillus macerans Schardinger 1905 are genetically and phenotypically distinct species. Thus, the phenotypic heterogeneity of the species B. circulans sensu lato is not due to inherent variability of genetically related strains but rather is due to inclusion of genetically unrelated organisms in this taxon.

tRNA 2-phosphotransferase, catalyzes the final step in yeast tRNA splicing: the transfer of the 2-PO(4) from the splice junction to NAD(+) to form ADP-ribose 1-2cyclic phosphate and ...

The 20 kDa Bacillus circulans Bcx is a well-studied endoxylanase with a beta-jellyroll fold that places its N- and C-termini in salt bridge contact. initial experiments verified that Bcx could be circularly permuted. by PCR methods to introduce new termini in loop regions while linking its native termini directly or via one or two glycines. Subsequently, a library Of circular permutants, generated by random DNase cleavage of the circularized Bcx gene, was screened for xylarlase activity oil xylan in Congo Red-stained agar. Analysis of 35 unique active circular permutants revealed that, while many of the new termini were introduced in external loops as anticipated, a surprising number were also located within beta-strands. Furthermore, several permutations placed key catalytic residucs at or near the new termini with minimal delecterious effects Oil activity and, in one case, a 4-fold increase. The structure of one permutant Was determined by X-ray crystallography, whereas three others were ...

Regulation and production of Fibrinolytic enzymes from bacterial sources especially from Bacillus strains has taken a leading role in the medical sciences for the treatment of cardiovascular disorders as it removes thrombus or clots adding to its significant role in curing human health issues saving millions. Significant progress has been made during the last few years on the studies of fibrinolytic enzymes in identifying, cloning, purification, characterization and overproduction of these for commercialization in medical sciences and in fields like detergents development. Production of fibrinolytic enzyme from Bacillus circulans was done using Nutrient broth medium. In addition, a strong fibrinolytic enzyme was purified from the cultivation media. The purified enzyme was almost homogeneous with other species of same genus, as examined by SDS−PAGE and sephadex G-75 column chromatography. The enzyme had an optimal pH of 7-12, an optimal temperature of 50 °C, for fibrin hydrolysis. The molecular mass

Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in stomach 1 tissue. Antibody staining with HPA072204 in immunohistochemistry.

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The bacterium Legionella pneumophila is the responsible agent for Legionnaires disease and has recently been shown to harbor a gene encoding a kinase that confers resistance to the aminoglycoside antibiotic spectinomycin (Suter, T. M., Viswanathan, V. K., and Cianciotto, N. P. (1997) Antimicrob. Agents Chemother. 41, 1385-1388). We report the overproduction, purification, and characterization of this spectinomycin kinase from an expressing system in Escherichia coli. The purified protein shows stringent substrate specificity for spectinomycin with Km = 21.5 microM and kcat = 24.2 s-1 and does not bind other aminoglycosides including kanamycin, amikacin, neomycin, butirosin, streptomycin, or apramycin. Purification of spectinomycin phosphate followed by characterization by mass spectrometry and 1H, 13C, and 31P NMR established the site of phosphorylation to be at the hydroxyl group at position 9. Thus this enzyme is designated APH(9)-Ia (where APH is aminoglycoside kinase). The enzyme was inactivated by

Expression of FBXW11 (BTRC2, BTRCP2, Fbw11, Fbw1b, FBXW1B, Hos, KIAA0696) in heart muscle tissue. Antibody staining with HPA072204 in immunohistochemistry.

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Two clinical isolates of Pseudomonas aeruginosa, one a pyocin type 5 strain from Atlanta, could transfer gentamicin resistance by conjugation. Donor and recipient strains inactivated gentamicin by acetylation. The R plasmids, pMG1 and pMG2, also determined resistance to sisomicin, another substrate of gentamicin acetyltransferase I, sulfonamides, and streptomycin, but not resistance to kanamycin, neomycin, tobramycin, butirosin, or BB-K 8. They were transmissible to many strains of P. aeruginosa, including a Rec− strain, but not to Escherichia coli or other enterobacteriaceae. These R plasmids were compatible with R plasmids transmissible to P. aeruginosa from E. coli, including members of C, N, P, and W incompatibility groups. From a strain carrying pMG1 and a compatible plasmid, pMG1 was transferred independently but transfer of the second plasmid often resulted in cotransfer of pMG1. In contrast, pMG1 and pMG2 were incompatible with pseudomonas R plasmids R931 and R3108, and with R931 they ...

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1CDG: Nucleotide sequence and X-ray structure of cyclodextrin glycosyltransferase from Bacillus circulans strain 251 in a maltose-dependent crystal form.

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Wiki-Pi: a web resource for human protein-protein interactions. It shows genes and PPIs with information about pathways, protein-protein interactions (PPIs), Gene Ontology (GO) annotations including cellular localization, molecular function and biological process, drugs, diseases, genome-wide association studies (GWAS), GO enrichments, PDB ID, Uniprot ID, HPRD ID, and word cloud from pubmed abstracts.

Tn5 ATCC ® 87140™ Designation: pBSL97 TypeStrain=False Application: contains easily purifiable cassette(s) for construction aminoglycoside phosphotransferase kanamycin resistance, neomycin resistance

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The aminoglycoside phosphotransferases (APHs) are responsible for the bacterial inactivation of many clinically useful aminoglycoside antibiotics. We report the characterization of an enterococcal enzyme, APH(3)-IIIa, which inactivates a broad spectrum of aminoglycosides by ATP-dependent O-phosphorylation. Overproduction of APH(3)-IIIa has permitted the isolation of 30-40 mg of pure protein/(L of cell culture). Purified APH(3)-IIIa is a mixture of monomer and dimer which is slowly converted to dimer only over time. Dimer could be dissociated into monomer by incubation with 2-mercaptoethanol, suggesting that dimerization is mediated by formation of disulfide bond(s). Both monomer and dimer show Km values in the low micromolar range for good substrates such as kanamycin and neomycin, and kcat values of 1-4 s-1. All aminoglycosides show substrate inhibition except amikacin and kanamycin B. Determination of minimum inhibitory concentrations indicates a positive correlation between antibiotic activity and

1OT1: The fully conserved Asp residue in Conserved sequence region I of the alpha-amylase Family is crucial for the Catalytic Site Architecture and Activity

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Bacillus circulans subsp. circulans clone Bcir-1R 16S ribosomal RNA gene,partial sequence; 16S-23S internal transcribed spacer, complete sequence;and 23S ribosomal RNA gene, partial ...

In 1730, the Inquisition of Lisbon arrested José Francisco, an enslaved man raised in West Africa, who had learned in Brazil the art and craft of making amulets known as bolsas de mandinga. In their composition, use, and afterlives in the Inquisition the bolsas reveal the deep and mutually transformative spiritual and material connections that the slave trade engendered between Europeans and Africans in the early modern Atlantic World. Created and used in parallel to similar objects made elsewhere on the continent, once deemed fetishes and now considered central to the canon of African art, they waged a spirited battle against the witchcraft of the slave trade.. Cécile Fromont is an associate professor in the history of art department at Yale University. Her writing and teaching focus on the visual, material, and religious culture of Africa and Latin America with a special emphasis on the early modern period (ca 1500-1800) and on the Portuguese-speaking Atlantic World.. Her first book, The Art ...