The objective of this study was to review the literature on bronchoalveolar lavage fluid cell profiles in healthy children and adolescents, as well as on the use of BAL as a diagnostic and follow-up tool for lung disease patients in this age bracket. To that end, we used the Medline database, compiling studies published between 1989 and 2009 employing the following MeSH descriptors (with Boolean operators) as search terms: bronchoalveolar lavage AND cytology OR cell AND child. In healthy children, the cell profile includes alveolar macrophages (> 80%), lymphocytes (approximately 10%), neutrophils (approximately 2%) and eosinophils (< 1%). The profile varies depending on the disease under study. The number of neutrophils is greater in wheezing children, especially in non-atopic children, as well as in those with pulmonary infectious and inflammatory profiles, including cystic fibrosis and interstitial lung disease. Eosinophil counts are elevated in children/adolescents with asthma and can reach ...
TY - JOUR. T1 - Increased angiostatin levels in bronchoalveolar lavage fluids from ARDS patients and from human volunteers after lung instillation of endotoxin. AU - Lucas, Rudolf. AU - Lijnen, H. Roger. AU - Suffredini, Anthony F.. AU - Pepper, Michael S.. AU - Steinberg, Kenneth P.. AU - Martin, Thomas R.. AU - Pugin, Jérôme. PY - 2002/1/1. Y1 - 2002/1/1. N2 - Acute respiratory distress syndrome (ARDS) is characterized by a disruption of the alveolar-capillary barrier, due to both an epithelial and an endothelial dysfunction. Whereas epithelial apoptosis seems to be mainly mediated by Fas ligand, the mediators of endothelial damage remain to be identified. Angiostatin, a powerful inhibitor of angiogenesis in vivo, also specifically induces apoptosis in endothelial cells. The concentration of various enzymes that cleave angiostatin from plasminogen was reported to be significantly increased in bronchalveolar lavage (BAL) fluids from patients with ARDS. Therefore, in this study, we ...
PAN Czytelnia Czasopism, Cytological evaluation of tracheal aspirate and broncho-alveolar lavage fluid in comparison to endoscopic assessment of lower airways in horses with recurrent airways obstruction or inflammatory airway disease - Polish Journal of Veterinary Sciences
The usefulness of bronchoalveolar lavage in the diagnosis of pulmonary infiltrates in the immunosuppressed patient was studied in 97 patients. In immunosuppressed patients, the available diagnostic procedures are often invasive and have variable yield and a potential for serious complications. Bronchoalveolar lavage had an overall diagnostic yield of 66% (61 of 92 diseases). It was most effective in the diagnosis of opportunistic infections, including infection with Pneumocystis carinii (18 of 22 cases), cytomegalovirus pneumonia (10 of 12 cases), fungal pneumonia (5 of 6 cases), and mycobacterial disease (4 of 5 cases). The technique was also helpful in confirming pulmonary hemorrhage (7 of 9 cases) but was less useful for diagnosing malignancy (10 of 22 cases) and drug-induced toxicity (6 of 15 cases). Findings of bronchoalveolar lavage were additive with those of transbronchial biopsies, brushings, and washings in the diagnosis of most of the diseases. The procedure was safe, even in ...
en] An increased production of NO* and peroxynitrite in lungs has been suspected during acute lung injury (ALI) in humans, and recent studies provided evidence for an alveolar production of nitrated compounds. We observed increased concentrations of nitrites/nitrates, nitrated proteins and markers of neutrophil degranulation (myeloperoxidase, elastase and lactoferrine) in the fluids recovered from bronchoalveolar lavage fluids (BALF) of patients with ALI and correlated these changes to the number of neutrophils and the severity of the ALI. We also observed that BALFs stimulated the DNA-binding activity of the nuclear transcription factor kappa B (NF-kappaB) as detected by electrophoretic mobility shift assay in human alveolar cells (A549) and monocytes (THP1). The level of activation of the NF-kappaB-binding activity was correlated to the concentration of nitrated proteins and myeloperoxidase. Furthermore, in vitro studies confirmed that NO*-derived species (peroxynitrite and nitrites) and the ...
Several components of cellular and humoral immunity were examined in bronchoalveolar lavage fluid and blood of 15 patients with the acquired immunodeficiency syndrome, and the results were compared to data from 25 healthy controls (including 5 asymptomatic homosexual men). Compared with that of controls, bronchoalveolar lavage fluid from patients tended to have more lymphocytes and significantly more neutrophils; a lower OKT4/OKT8 ratio, due to an increase in total OKT8 cells; and normal total OKT4 cell counts, despite a significant decrease in numbers of OKT4 cells in peripheral blood. Patients also had significantly more IgG-releasing cells and higher IgG levels than controls in lavage fluid. These data show that, in the lung lining fluid of patients with the acquired immunodeficiency syndrome, significant alterations in cellular and humoral immunity exist that differ in several important respects from immunity in controls and from corresponding changes in patients peripheral blood. ...
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Patrolling Monocytes Promote the Pathogenesis of Early Lupus-Like Glomerulonephritis Researchers found that glomerulonephritis was driven by TLRs but, remarkably, proceeded independently of immune complexes. Rather, disease in three different mouse models and patients with systemic lupus erythematosus was characterized by glomerular accumulation of patrolling monocytes, a cell type with an emerging key function in vascular inflammation. [J Clin Invest] Full Article Lipopolysaccharide Inhalation Recruits Monocytes and Dendritic Cell Subsets to the Alveolar Airspace The authors used lipopolysaccharide inhalation to induce acute inflammation in healthy volunteers and examined the impact on bronchoalveolar lavage fluid and blood mononuclear phagocyte repertoire. Classical monocytes and two dendritic cell subsets were expanded in bronchoalveolar lavage fluid eight hours after lipopolysaccharide inhalation. [Nat Commun] Full Article FcγRIIb Differentially Regulates Pre-Immune and Germinal Center B ...
Background: Cytologic examination of specimens obtained from the respiratory tract is a lung cancer diagnostic procedure with high specificity, but moderate sensitivity. The use of molecular biomarkers may enhance the sensitivity of cytologic examination in the detection of lung cancer. Methods: Complement factor H, a protein secreted by lung cancer cells, was quantified in a series of bronchoalveolar lavage supernatants from lung cancer patients and patients with non-malignant respiratory diseases. Albumin, total protein content, and hemoglobin were also analyzed. Results were validated in independent sets of bronchoalveolar lavage and sputum supernatants. Results: There was a significantly higher concentration of factor H in bronchoalveolar lavage samples from lung cancer patients. The sensitivity and specificity of the factor H test was 82% and 77%, respectively. These results were validated in an independent set of patients with nearly identical results. Furthermore, 70% and 45% of ...
The purpose of this study is to explore the correlations of interleukin 36 (IL-36) and Soluble B7-H3 (sB7-H3) levels in bronchoalveolar lavage fluid (BALF) with clinical characteristics and laboratory findings. A total of 35 children with M. pneumnoiae pneumonia (MPP) and 15 control subjects were enrolled. BALF concentrations of sB7-H3 and IL-36 were detected using enzyme-linked immunosorbent assays and clinical profiles of children with MPP were obtained. Children with MPP had significantly higher levels of sB7-H3 and IL-36 compared to control subjects (both P < 0.05). Meanwhile, children with pleural effusion had significantly higher levels of sB7-H3 and IL-36 compared to children without pleural effusion (both P < 0.05). BALF concentration of sB7-H3 was strongly associated with concentration of IL-36 (r = 0.796, P < 0.0001) and sB7-H3 was correlated with duration of fever (r = 0.427, P = 0.11) and length of stay (r = 0.345, P = 0.043). Both concentrations of sB7-H3 and IL-36 were significantly
Vitamin E acetate was universally found in bronchoalveolar lavage fluid samples from patients with vaping lung injury, providing the first direct evidence of vitamin E acetate at the site of injury.
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Cytokine Levels In Bronchoalveolar Lavage Fluid (Balf) In Patients With Systemic Sclerosis (SSc). In comparison to other diseases, SSc patients present a spe...
Bronchoscopy with bronchoalveolar lavage. Refractory pneumonitis. A 69-year-old man status post trauma, slightly prolonged respiratory failure status post tracheostomy, requires another bronchoscopy for further evaluation of refractory pneumonitis.
TY - JOUR. T1 - Bronchoalveolar lavage in the study of asthma. AU - Rennard, S. I.. AU - Thompson, A. B.. AU - Floreani, A. A.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026770648&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026770648&partnerID=8YFLogxK. U2 - 10.1378/chest.102.1.1. DO - 10.1378/chest.102.1.1. M3 - Editorial. C2 - 1623734. AN - SCOPUS:0026770648. VL - 102. SP - 1. EP - 2. JO - Chest. JF - Chest. SN - 0012-3692. IS - 1. ER - ...
... ,ARUP Laboratories is a national reference laboratory and a worldwide leader in innovative laboratory research and development. ARUP offers an extensive test menu of highly complex and unique medical tests in clinical and anatomic pathology. Owned by the University of Utah, ARUP Laboratories client,medicine,medical supply,medical supplies,medical product
bronchoalveolar definition: Adjective (comparative more bronchoalveolar, superlative most bronchoalveolar) 1. Relating to both the bronchi and the alvioli of the lungs...
The pathophysiological role of inflammation in lung diseases including asthma and chronic obstructive pulmonary disease is well established. Quantifying lung inflammation is relevant for the management of inflammatory airway diseases as it may indicate that pharmacological intervention is required before symptom onset and reduction in lung function. Moreover, monitoring of airway inflammation might be useful in the follow-up of patients with asthma and COPD, and for guiding pharmacological therapy. Quantification of pulmonary inflammation is currently based more on invasive methods including the analysis of broncho-alveolar lavage fluid, bronchoscopy, and bronchial biopsies, semi-invasive methods such as sputum induction, and the measurement of inflammatory biomarkers in plasma and urine, which are likely to reflect systemic rather than lung inflammation. Interest in the identification of non-invasive biomarkers for inflammatory airway diseases has been growing. Along these years less invasive ...
Pulmonary infection is normally a major reason behind mortality and morbidity as well as the magnitude from the lung inflammatory response correlates with affected person survival. and peptidoglycan (PGN) implemented intratracheally significantly elevated the amounts of neutrophils retrieved in the bronchoalveolar lavage liquid (BALF). F344 got approximately 10-flip even more neutrophils in the BALF weighed against DA (< 0.001) and higher BALF concentrations of total proteins tumor necrosis aspect-α and macrophage inflammatory proteins 2. LTA/PGN administration in DA×F344 congenic strains (with LTA/PGN created significantly higher degrees of keratinocyte-derived chemokine and macrophage inflammatory proteins 2 than alveolar macrophages from DA rats. The distinctions had been linked to differential mitogen-activated proteins kinase phosphorylation. We conclude the fact that factors adding to irritation could be site and problem dependent. A better knowledge of site-specific irritation can lead ...
To transport BAL samples from the field, pour samples into EDTA tubes or dilute 50:50 with grain alcohol or Vodka (100 Proof) and transport in clot tubes. Dont drink and drive! ...
This project will identify the characteristic immune cell profiles for two patient groups with allergic reactions with or without specific IgE in serum, also compared with healthy controls.
The figure above shows the overall detectability of the specified assay in a range of different mouse sample types. Detectability in each sample matrix is presented as the mean % of all samples included in the validation studies that could be measured above the limit of detection (LOD).. Dilution of samples may be necessary for some sample matrices other than plasma or serum to achieve optimal assay performance - the data shown above represents the values obtained at the optimal dilution identified in each case. Details regarding possible dilutions should be discussed with your Olink representative prior to commencing the study. The data is compiled from multiple studies comprising a range of healthy and disease samples, including samples from genetically modified mouse lines in some cases.. (Note: CSF = cerebrospinal fluid; BAL = bronchoalveolar lavage).. ...
Source: Adapted from the National Institutes of Health. What does the term "lavage" mean? The term "lavage" refers to washing out of an organ, especially the stomach. To find out more about this term, please search the news section of this website for related articles and information.. ...
Trimesulfin Balsámico is a medicine available in a number of countries worldwide. A list of US medications equivalent to Trimesulfin Balsámico is available on the Drugs.com website.
Confused on this one. Inpatient visist: When a Intensivist does a procedure of BAL Lavage and its done on both lungs. Is this report twice with CPT 3
differential cell counting in mixed cultures - posted in Tissue and Cell Culture: hello, i will be culturing neurons and macrophages together in a mixed culture. does anyone know of any methods for counting one cell type alone?
Also known as: bal, Bal., Bal, BaL, Ba.L, Ba-L. An, Ba-L, BA¶l, BAl, BAL., BAL, BA-L, B=Al, B.A.L., B.A.L, B. A. L., B-aL, B-AL ...
It was initially thought the use of chemical-grade silicone was limited to implants made during the past decade, but the TGA today said there were indications from European regulators the problem extended back to the late 1990s.
Data & statistics on Acute Lung Injury: MRNA expression of Interleukin-8 in bronchoalveolar lavage fluid cells before injury and after injury. NG, acute lung injury with normoglycemia group; HG, acute lung injury with hyperglycemia group; HG-VI, acute lung injury with hyperglycemia treated with intravenous insulin group; HG-AI, acute lung injury with hyperglycemia treated with aerosolized insulin group; IL-8, interleukin-8. Boxes extend ..., Particle-induced acute lung injury. Results obtained from analysis performed on bronchoalveolar lavage fluid samples recovered from animals at 24 h post-exposure. Animals were exposed by intratracheal instillation to: saline ( E); OFA100 ( C); OFA400 (P); ROFA#6 ( 1); or ARD ([). Various biomarkers of acute lung injury were assessed such as: A) edema/secretory activity (protein/mL); B) edema (albumin/mL); ..., Inhaltsverzeichnis Einleitung Hypothesen Theoretische Grundlagen der Hypothesen Patienten und Methodik Patienten Pneumonie Acute lung injury / Acute
TY - JOUR. T1 - Removal of bronchoalveolar cells augments the late eosinophilic response to segmental allergen challenge. AU - Hunt, L. W.. AU - Gleich, G. J.. AU - Kita, H.. AU - Weiler, D. A.. AU - Schroeder, D. R.. AU - Vuk Pavlovic, Z.. AU - Sur, Sanjiv. PY - 2002. Y1 - 2002. N2 - Background: In patients with quiescent asthma, macrophages are the most prevalent cells recovered by bronchoalveolar lavage (BAL). Through activation via their FcεRII receptors or by acting as antigen-presenting cells, macrophages could, in theory, promote the late airway response to allergen. Objective: In order to investigate the importance of macrophages and other airway luminal cells in inducing the late airway response, a novel washout experiment was designed. Methods: Five patients with ragweed-allergic asthma underwent bronchoscopy and segmental bronchial challenge with either normal saline or short ragweed extract in two segments of one lung. In a third segment of the opposite lung, 12 successive BALs ...
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in patients (pts) with hematologic malignancies. As culture-based methods only yield results in a minority of patients, using non-culture-based methods for detection of aspergillosis in clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL) samples with polymerase chain reaction (PCR) is promising, however, the influence of current antifungal drugs on the performance of this diagnostic tool remains controversial.. The aim of the trial is to elucidate on the performance of BAL PCR under antifungal treatment.. Patients with high risk of invasive aspergillosis and lung infiltrates are sampled via BAL, the sample is analyzed for fungal DNA by Apsergillus specific PCR. Clinical data including treatment data is assessed and evaluated. ...
This study was designed to determine whether cell populations in bronchoalveolar lavage fluid represent a reflection of disease activity in sarcoidosis. Bronchoalveolar lavage fluid cells were obtained from 22 patients with sarcoidosis and from 10 normal control subjects and investigated by immunocytological methods. A panel of monoclonal antibodies was used to determine the relative proportions of phenotypically distinct subsets of macrophages and lymphocytes in the patients with sarcoidosis and to correlate them with clinical indices, such as disease duration, serum angiotensin converting enzyme, the chest radiograph, and results of pulmonary function tests. Patients with sarcoidosis had a higher percentage than the normal subjects of macrophage like cells expressing RFD1 (a class II associated antigen preferentially expressed by dendritic cells), an epithelioid cell antigen (RFD9), and a circulating monocyte antigen (UCHMI). The increase in RFD1+ cells appeared to be due to detection of ...
TY - JOUR. T1 - Noninvasive quantification of airway inflammation following segmental allergen challenge with functional MR imaging. T2 - A proof of concept study. AU - Renne, Julius. AU - Hinrichs, Jan. AU - Schönfeld, Christian. AU - Gutberlet, Marcel. AU - Winkler, Carla. AU - Faulenbach, Cornelia. AU - Jakob, Peter. AU - Schaumann, Frank. AU - Krug, Norbert. AU - Wacker, Frank. AU - Hohlfeld, Jens M.. AU - Vogel-Claussen, Jens. PY - 2015/1/1. Y1 - 2015/1/1. N2 - Purpose: To evaluate oxygen-enhanced T1-mapping magnetic resonance (MR) imaging as a noninvasive method for visualization and quantification of regional inflammation after segmental allergen challenge in asthmatic patients compared with control subjects.Materials and After institutional review board approval, nine asthmatic Methods: and four healthy individuals gave written informed consent. MR imaging (1.5 T) was performed by using an inversion-recovery snapshot fast low-angle shot sequence before (0 hours) and 6 hours and 24 hours ...
Myeloid dendritic cells (DCs) are increased in the airway wall of patients with chronic obstructive pulmonary disease (COPD), and postulated to play a crucial role in COPD. However, DC phenotypes in COPD are poorly understood. Function-associated surface molecules on bronchoalveolar lavage fluid (BALF) DCs were analyzed using flow cytometry in current smokers with COPD, in former smokers with COPD and in never-smoking controls. Myeloid DCs of current smokers with COPD displayed a significantly increased expression of receptors for antigen recognition such as BDCA-1 or Langerin, as compared with never-smoking controls. In contrast, former smokers with COPD displayed a significantly decreased expression of these receptors, as compared with never-smoking controls. A significantly reduced expression of the maturation marker CD83 on myeloid DCs was found in current smokers with COPD, but not in former smokers with COPD. The chemokine receptor CCR5 on myeloid DCs, which is also important for the uptake and
Background. In acute respiratory distress syndrome or pneumonia, a procoagulant shift is observed in bronchoalveolar lavage fluid (BALF). The effect of a primarily extrapulmonary infection on coagulation and fibrinolysis in the pulmonary compartment is unclear. Methods. In 35 patients, 87 bronchoalveolar lavages were performed on the day of operation for secondary peritonitis (day 0) and on days 2 and 3 after surgery. Two noninfectious control groups were included: subjects undergoing bronchoalveolar lavage after elective surgery (n = 8) and those undergoing long-term mechanical ventilation (n = 10). Results. In BALF from patients with peritonitis, a tissue factor (TF)/factor VIIaâ€"mediated activation of coagulation was shown (high levels of thrombin-antithrombin complexes). Levels of fibrinolysis activators decreased rapidly after day 0, whereas levels of inhibitors increased. The net effect was reduced fibrinolysis (plasminogen activator activity). The sequential comparison of plasma ...
We report the case of a 55-year-old woman, former smoker since September 2012 (18 pack-years), with a history of allergic rhinoconjunctivitis and bronchial asthma. No infiltrates were observed in a chest X-ray performed in 2012. Skin prick tests showed sensitization to cat dander and grass and olive pollen.. She was admitted in 2014 for an acute episode of dyspnea, breath sounds, and non-productive cough without fever. Chest X-ray revealed an alveolointersticial pattern predominantly in the lung bases. Chest computed tomography showed bilateral patchy ground glass infiltrates. Bronchoscopy was performed with transbronchial biopsy and bronchoalveolar lavage: neutrophils: 11%; eosinophils: 85.9%; lymphocytes: 0.6%. Biopsy showed a histological pattern of pulmonary eosinophilia suggestive of chronic eosinophilic pneumonia (CEP), with a focal pattern typical of organizing pneumonia. Lung function tests highlighted a restrictive pattern with carbon monoxide diffusion changes: FVC 1830 - 59%; FEV1 ...
Lu et al prospectively studied 165 patients with culture-confirmed (bronchoalveolar lavage samples), ventilator-associated pneumonia (VAP) caused by either Pseudomonas aeruginosa or Acinetobacter baumannii.
Table II shows differential count for the three patients. There is increased neutrophil count after CPB with predominance of macrophages in both samples.. DISCUSSION. Inflammation may be understood as a protective response against the consequences of tissue aggression, organ dysfunction and cell necrosis 10, being a systemic process present even in the absence of systemic inflammatory response syndrome (SIRS) 11. However, if SIRS evolves to organ dysfunction, the inflammatory response may be noxious 10 with pulmonary function changes, renal failure, multiorgan failure syndrome (MOFS) and shock 11.. Postoperative pulmonary dysfunction is frequent and contributes to morbidity, mortality and hospitalization-related costs 12. The understanding of postoperative pulmonary dysfunction pathophysiology is critical for postoperative clinical pulmonary complication evaluation and the definition of therapeutic regimens.. Postoperative pulmonary dysfunction pathogenesis is associated to changes in gas ...
Case Report Our case is a 4 month old, Sudanese boy, residing in Qatar, who presented to our hospital with fever for two weeks. He remained febrile in the hospital for two weeks, despite receiving Intravenous antibiotics for presumed partially treated meningitis. He also received IVIG for the suspicion of atypical Kawasaki. Computerised tomography of the head and abdomen and bone marrow studies were also inconclusive. The mother subsequently revealed that a previous child had died during infancy with prolonged fever and no specific diagnosis. This alerted the treating physicians to the possibility of immunodeficiency. Our patient had received BCG vaccine at birth without any complications, and three gastric aspirate samples were negative for tuberculosis. A broncho-alveolar lavage was performed, despite the absence of any respiratory symptoms, to exclude any unusual organisms causing prolonged fever. The broncho-alveolar lavage revealed mycobacterium tuberculosis which was resistant to Isoniazid ...
Metagenomic technologies enable the study of microbial genetic material in human biomedical sample types such as stool, nasal, oral, urogenital, skin and bronchoalveolar lavage samples. In addition, environmental samples such as soil, water, air and biofilms can be also be analyzed. Metagenomic data can not only be used to examine healthy microbiomes and shed light on causes, effects, and future therapies for a variety of diseases, but it can also be useful to help understand the environments microbial biodiversity. QIAGEN provides next-generation sequencing technologies for metagenomics, as well as qPCR assays and arrays for verification of sequencing results and screening for specific bacterial species, virulence factor genes, and antibiotic resistance genes ...
TY - JOUR. T1 - Intratracheal administration of endotoxin and cytokines. T2 - VIII. LPS induces E-selectin expression; anti-E-selectin and soluble E-selectin inhibit acute inflammation. AU - Ulich, T. R.. AU - Howard, S. C.. AU - Remick, D. G.. AU - Yi, E. S.. AU - Collins, T.. AU - Guo, K.. AU - Yin, S.. AU - Keene, J. L.. AU - Schmuke, J. J.. AU - Steininger, C. N.. AU - Welply, J. K.. AU - Williams, J. H.. PY - 1994. Y1 - 1994. N2 - E-selectin is an inducible endothelial adhesion molecule that binds neutrophils. E-selectin mRNA is not constitutively detectable in the lungs of rats. Intratracheal injection of LPS induces pulmonary E-selectin mRNA expression at 2-4 h. Intratracheal injection of LPS followed at 2 and 4 h by intravenous injection of mouse F(ab) 2 or F(ab) anti-E-selectin monoclonal antibody inhibits the emigration of neutrophils into the bronchoalveolar space at 6 h by 50-70% . TNF and IL-6 bioactivity are not decreased in bronchoalveolar lavage fluid after treatment with ...
BioAssay record AID 423142 submitted by ChEMBL: Effect on pulmonary inflammation in BALB/c mouse lung assessed as change in peribronchiolar inflammation after 5 days by histopathological analysis.
Asthma is a chronic respiratory disease that is characterized by airway inflammation, bronchoconstriction and the influx of pro-inflammatory cells, mostly eosinophils in the lung tissue and bronchoalveolar space. Amongst the many physiopathological roles attributed to endothelins (ETs), one is to modulate pulmonary functions. It is established that Balb/c mice develop allergen-induced Th2-cytokine gene expression, airway inflammation and hyper-responsiveness whereas C57Bl/6 mice are much less reactive. In the present study, we investigated the roles of ETs in these two murine models of allergic asthma (AA). Mice were sensitized and challenged with either saline (S) and/or ovalbumin (O) over 6 weeks (groups S/S and O/O) and treated chronically with ABT-627 or its vehicle. Twenty-four hours after the last sensitization, challenged mice developed a marked airway inflammatory response characterized by the accumulation of total inflammatory cells (a 21-fold increase) in the bronchoalveolar space, ...
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Recent studies have revealed that bronchoalveolar lavage (BAL) fluid contains previously unappreciated communities of bacteria. In vitro and in vivo studies have shown that host inflammatory signals prompt bacteria to disperse from cell-associated biofilms and adopt a virulent free-living phenotype. The proportion of the lung microbiota that is cell-associated is unknown. Forty-six BAL specimens were obtained from lung transplant recipients and divided into two aliquots: whole BAL and acellular BAL, the latter processed with a low-speed, short-duration centrifugation step. Both aliquots were analyzed via bacterial 16S rRNA gene pyrosequencing. The BAL specimens represented a wide spectrum of lung health, ranging from healthy and asymptomatic to acutely infected. Bacterial signal was detected in 52% of acellular BAL aliquots, fewer than were detected in whole BAL (96%, p ≤ 0.0001). Detection of bacteria in acellular BAL was associated with indices of acute infection [BAL neutrophilia, high total
Principal Investigator:HAYASHI Seiji, Project Period (FY):1992 - 1993, Research Category:Grant-in-Aid for General Scientific Research (C), Research Field:Respiratory organ internal medicine
An alteration in the balance between a T-helper type 2 cell (Th2) response and a Th1 response may predispose to the development of bronchial asthma. Interleukin-18 (IL-18) has an ability to promote both Th1 and Th2 responses, depending on the surrounding cytokine environment. Reactive oxygen species (ROS) play a crucial role in the pathogenesis of airway inflammation and hyperresponsiveness. Recent studies have demonstrated that antioxidants are able to reduce airway inflammation and hyperreactivity in animal models of asthma. In this study, we used a C57BL/6 mouse model of allergic asthma to examine the effects of antioxidants on the regulation of IL-18 expression. Our present study with ovalbumin-induced murine model of asthma revealed that ROS production in cells from bronchoalveolar lavage fluids was increased and that administration of l-2-oxothiazolidine-4-carboxylic acid or α-lipoic acid reduced the increased levels of ROS, the increased expression of IL-18 protein and mRNA, airway ...
... u. ... .... _n of lung parenchyma by CD8+ cells ~hl!eOI~cut immunohistological observations f!CU:ii~J CD8+ infiltrate in the group of continued to ~ regularly exposed, and of CD4+ cells after 6 months in the lungs of were not. indicate that alveolitis in RP patients is a and its intensity might be modulated by to relevant antigens, amount of antigen of sensitization, thus explaining the BAL lymphocytosis observed in patients exposed to the specific antigens compared are noL Increased cells with supprcssor/cyin the lung of these patients is related to a local immunological response . These mechanisms may be relevant in the pathogenesis of HP.
This is because co-receptor CD4 amplifies the signal produced for the T-cell receptor by recruiting lymphocyte protein kinase Lck, which consequently triggers a cascade of gatherings leading to T-cell activation. Curiously, DHLA didnt downregulate CD4 from the surface area of human peripheral blood mononuclear cells [a hundred forty five]. Instead or Along with CD4 downregulation, the immunomodulatory Houses of LA may perhaps include the upregulation of cAMP in T-cells and organic killer cells [146]. Mobile migration and neovascularization were being also inhibited by LA (86 μg/day in consuming h2o) in c57/black mice injected with Kaposis sarcoma inside of a matrigel sponge, in addition to in nude mice injected with KS cells [147]. In the mouse product of bronchial asthma, dietary LA considerably attenuated airway hyper-responsiveness, reduced the eosinophil depend among the bronchoalveolar lavage cells, and considerably improved pathologic lesion scores with the lungs [148]. LA inhibits ...
Results C57 mice demonstrated a higher total bronchoalveolar lavage (BAL) and BAL lymphocyte count at 3 and 7 days after intraperitoneal infection compared with BALB mice. There were no differences in BAL cytokine production; however, we were able to demonstrate differences in CMV DNA load in the lungs of BALB mice compared with that of C57 mice. In addition, there appeared to be increased whole-lung production of the TH2 cytokine IL-10 in the BALB mice versus the C57 mice. ...