TY - THES. T1 - Chemical virology. T2 - decorating the interior of the cowpea chlorotic mottle virus. AU - Minten, Inge Jeannette. PY - 2011/4/4. Y1 - 2011/4/4. KW - METIS-283479. M3 - PhD Thesis - Research external, graduation external. SN - 978-90--9025926-0. PB - Radboud University Nijmegen. CY - Nijmegen. ER - ...

Positive-strand RNA viruses are the largest virus class and include many pathogens such as hepatitis C virus and the severe acute respiratory syndrome coronavirus (SARS). Brome mosaic virus (BMV) is a representative positive-strand RNA virus whose RNA replication, gene expression, and encapsidation have been reproduced in the yeast Saccharomyces cerevisiae. By using traditional yeast genetics, host genes have been identified that function in controlling BMV translation, selecting BMV RNAs as replication templates, activating the replication complex, maintaining a lipid composition required for membrane-associated RNA replication, and other steps. To more globally and systematically identify such host factors, we used engineered BMV derivatives to assay viral RNA replication in each strain of an ordered, genome-wide set of yeast single-gene deletion mutants. Each deletion strain was transformed to express BMV replicase proteins and a BMV RNA replication template with the capsid gene replaced by a ...

Shop RNA replication protein ELISA Kit, Recombinant Protein and RNA replication protein Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.

Read Remarkable variability of apple mosaic virus capsid protein gene after nucleotide position 141, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Under conditions of low ionic strength and a pH ranging between about 3.7 and 5.0,solutions of purified coat proteins of cowpea chlorotic mottle virus (CCMV) form sphericalmultishell structures in the absence of viral RNA. The outer surfaces of the shells in thesestructures are negatively charged, whereas the inner surfaces are positively charged due to a disordered cationic N-terminal domain of the capsid protein, the arginine-rich RNA-binding motif that protrudes into the interior. We show that the main forces stabilizing these .... ...

Aniagyei, Stella E. and Kennedy, Chelsea J. and Stein, Barry and Willits, Deborah A. and Douglas, Trevor and Young, Mark J. and De, Mrinmoy and Rotello, Vincent M. and Srisathiyanarayanan, D. and Kao, C. Cheng and Dragnea, Bogdan. (2009) Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids. Nano Letters, 9 (1). pp. 393-398. ISSN 1530-6984 ...

The genome is segmented and tripartite. The segments are distributive among 3 particle types of different sizes. The genome consists of three segments to four segments of linear, positive-sense, single-stranded RNA. The virion may contain minor species of non-genomic nucleic acid. Although the encapsidated nucleic acid is mainly of genomic origin, the virions may also contain subgenomic RNA. Subgenomic RNA is the mRNA (RNA-4, a subgenomic fragment of RNA-3) derived from genomic RNA-3. The complete genome is fully sequenced and is 8620-8800 nucleotides long. RNA-1 has been fully sequenced and 3158-3372-3900 nucleotides long; RNA-2 has been fully sequenced and 2799-3032-3400 nucleotides long; RNA-3 is sequenced but only an estimate is given and the sequence is 2117-2296-2600 nucleotides long; RNA-4 has been fully sequenced and is 800 nucleotides long. The genome has a base ratio of 24.4-25.95-28 % guanine; 24.9-26.05-27 % adenine; 19-20.62-22.2 % cytosine; 24-27.42-29 % uracil. The genome has a ...

The genome is segmented and tripartite. The segments are distributive among 3 particle types of different sizes. The genome consists of three segments to four segments of linear, positive-sense, single-stranded RNA. The virion may contain minor species of non-genomic nucleic acid. Although the encapsidated nucleic acid is mainly of genomic origin, the virions may also contain subgenomic RNA. Subgenomic RNA is the mRNA (RNA-4, a subgenomic fragment of RNA-3) derived from genomic RNA-3. The complete genome is fully sequenced and is 8620-8800 nucleotides long. RNA-1 has been fully sequenced and 3158-3372-3900 nucleotides long; RNA-2 has been fully sequenced and 2799-3032-3400 nucleotides long; RNA-3 is sequenced but only an estimate is given and the sequence is 2117-2296-2600 nucleotides long; RNA-4 has been fully sequenced and is 800 nucleotides long. The genome has a base ratio of 24.4-25.95-28 % guanine; 24.9-26.05-27 % adenine; 19-20.62-22.2 % cytosine; 24-27.42-29 % uracil. The genome has a ...

Cytoplasmic Protein Required For Replication Of Brome Mosaic Virus; S. Cerevisiae Is A Model System For Studying Replication Of Positive-strand RNA Viruses In Their Natural Hosts

Harrison Brome, the 20-year old brooding crooner released his debut EP Fill Your Brains in July 2016. The EP featured Spotify & blog favorites Sex Calls, Boy, Pools, Midnight Island, Gambling Hearts and title track Fill Your Brain. With the recent release of Body High EP, Bromes music is a powerful composition of work showcasing sophistication beyond his years. Its evident that the young Brome has been honing his craft; its poignant melodies work together with his harmonized howls for a hauntingly somber listening, evocative of the notoriously dark and gloomy weather of his hometown of Vancouver. This is just the beginning of the emotional roller coaster that is Harrison Brome.. ...

Principal Investigator：NOMURA Yasuyuki, Project Period (FY)：1990 - 1991, Research Category：Grant-in-Aid for General Scientific Research (B), Research Field：Biological pharmacy

Brome mosaic virus (BMV) is a small (28 nm, 86S), positive-stranded, icosahedral RNA plant virus belonging to the genus Bromovirus, family Bromoviridae, in the alphavirus-like superfamily. BMV commonly infects Bromus inermis (see Bromus) and other grasses, can be found almost anywhere wheat is grown, and thrives in areas with heavy foot or machinery traffic. It is also one of the few grass viruses that infects dicotyledonous plants; however, it primarily infects monocotyledonous plants, such as barley and others in the family Gramineae. BMV was first isolated in 1942 from bromegrass (Bromus inermis), had its genomic organization determined by the 1970s, and was completely sequenced with commercially available clones by the 1980s. The alphavirus-like superfamily includes more than 250 plant and animal viruses including Tobacco mosaic virus, Semliki forest virus, Hepatitis E virus, Sindbis virus, and arboviruses (which cause certain types of encephalitis). Many of the positive-strand RNA viruses ...

Daniel, Marie-Christine and Tsvetkova, Irina B. and Quinkert, Zachary T. and Murali, Ayaluru and De, Mrinmoy and Rotello, Vincent M. and Kao, C. Cheng and Dragnea, Bogdan. (2010) Role of Surface Charge Density in Nanoparticle-Templated Assembly of Bromovirus Protein Cages. ACS Nano, 4 (7). p. 3853. ISSN 1936-0851 Aniagyei, Stella E. and Kennedy, Chelsea J. and Stein, Barry and Willits, Deborah A. and Douglas, Trevor and Young, Mark J. and De, Mrinmoy and Rotello, Vincent M. and Srisathiyanarayanan, D. and Kao, C. Cheng and Dragnea, Bogdan. (2009) Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids. Nano Letters, 9 (1). pp. 393-398. ISSN 1530-6984 De, Mrinmoy and Rana, Subinoy and Akpinar, Handan and Miranda, Oscar R. and Arvizo, Rochelle R. and Bunz, Uwe H. F. and Rotello, Vincent M.. (2009) Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein. Nature Chemistry, 1 (6). p. 461. ...

Page contains details about doxorubicin-conjugated tobacco mosaic virus capsid protein mutant assemblies . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com

In the summer of 2005, Giau was a REU intern in Dr. C. Kaos lab at Texas A&M University. Her project was titled, Visualization of Fluorescent Brome Mosaic Virus RNA3 in an In-vivo Environment.. During my stay at Texas, I worked with a plant virus called Brome Mosaic Virus. In recent years, this virus has become a model system for positive strand RNA viruses.. The internship was a rewarding experience because it was my first time being exposed to scientific research on a larger scale. Within one lab there were at least 4-5 large scale projects going on. It was definately an action-packed environment for science majors.. Secondly, I was very amazed to see how these scientists were so dedicated to their work. The Post-doc that I worked under spent at least 12 hours a day in the lab during weekdays, and he was always in the lab on the weekends as well. Through this internship I found out that the lab is where I want to be in the future.. Giau graduated magna cum laude with a double major in ...

1I4B: Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter

BMV development was studied in well defmed physiological conditions, avoiding the use of antibiotics or excision of the roots. For an adequate labelling, to distinguish between host and viral RNAs, different periods of exposure to 32p were required, depending upon the post-inocu1ation time of infected plants and corresponding age of healthy controL Within the period of normal outlook of the plants, the rate of 32p· incorporation into BMV RNA was much higher than into barley nboso-mal RNA in infected tissue or in comparable healthy tissue.Analysis of the replicative structures isolated from infected plants revealed that only the three largest RNAs (A,K,B) had their own replicative intermediates (R I). This material was shown to con-tain a certain amount of intact viral RNA species, which were stable when treated with heat and for-mamide, excluding the possibility of hidden breaks. read more ...

By using the ability of the positive-strand RNA ((+)RNA) virus BMV to replicate in yeast it was previously shown that subunits of the LSm1-7 ring, as well as Pat1 and Dhh1 play an essential role in the transit of the BMV ...

POSTDOCTORAL POSITIONS IN VIRAL RNA REPLICATION, GENE EXPRESSION AND VIRUS-HOST INTERACTIONS Creative, highly motivated researchers are sought for postdoctoral positions studying the functions and interactions of viral and host factors in RNA replication, gene expression, and related events in positive-strand RNA viruses. Unique opportunities to identify and study relevant host factors are available through our demonstration that bromovirus and nodavirus RNA replicons can replicate and express foreign genes in yeast, allowing the use of powerful yeast genetics to identify pertinent host genes and to greatly facilitate other experiments (Janda and Ahlquist [1993] Cell 72:961-970; Quadt et al. [1995] PNAS 92:4892-4896; Price et al. (1996) PNAS 93:9465-9470). Bromoviruses and nodaviruses are well-studied viruses with excellent genetic and biochemical resources for in vivo and in vitro studies of fundamental replication mechanisms. Significant opportunities are available in several areas of ...

in fact, both the 1a and 2a genes of brome mosaic virus were expressed as , transgenes, and an RNA3 derivitave as a replication substrate. I was just , trying to make two points to the curious layperson: first, that it is possible , for a virus to cross kingdoms (beyond the bunyaviridae) and second, that it is , difficult, and is unlikely to occur without a good deal of human or evolutionary effort, , Cheers! Good answer...but as Terry Hanzlik pointed out, flock house nodavirus infects plants (done in your institution) AND insects, and can multiply in cells of a number of animal cell lines...so maybe some of the effort isnt needed! And its not only Bunyaviridae; also Reoviridae and Rhabdoviridae, and some other as yet unclassified animal-type viruses which multiply in pants [Freudian, that - mean PLANTS, of course!] and insects. And there is evidence accumulating to suggest certain Potyviridae multiply in plants and fungi, and someone in our institute is convinced a certain relatively obscure ...

Several Indiana Bureau of Motor Vehicles (BMV) branches are temporarily closed in order to reallocate staff to more accessible branches because of staff shortages.

TY - JOUR. T1 - Mutational Analysis of Cucumber Mosaic Virus Movement Protein Gene. AU - You, Jin Sam. AU - Baik, Hyung Suk. AU - Paek, Kyung Hee. PY - 1999/1/31. Y1 - 1999/1/31. N2 - The movement protein of cucumber mosaic virus (CMV) is required for cell-to-cell movement of viral RNA. The movement of viral RNA occurs through the plant intercellular connection, the plasmodesmata. The viral movement protein was known to be multifunctional. In this work, a series of deletion mutants of CMV movement protein gene were created to identify the functional domains. The mutated movement proteins were produced as inclusion body in E. coli, and purified and renatured. A polyclonal antibody was raised against the CMV-Kor strain (Korean isolate) movement protein expressed in E. coli. The ability of the truncated proteins to bind to ssRNA was assayed by UV cross-linking and gel retardation analyses. The results indicate that the domain between amino acids 118 and 160 of CMV movement protein is essential for ...

Machlomovirus is a genus of plant viruses, in the family Tombusviridae. Plants serve as natural hosts. There is currently only one species in this genus: the type species Maize chlorotic mottle virus (MCMV), which causes significant losses in maize production worldwide. MCMV was first identified in the U.S. state of Kansas causing corn lethal necrosis, a severe disease that negatively affects all stages of development for maize plants. Group: ssRNA(+) Order: Unassigned Family: Tombusviridae Genus: Machlomovirus Maize chlorotic mottle virus Viruses in Machlomovirus are non-enveloped, with icosahedral and spherical geometries, and T=3 symmetry. The diameter is around 28-34 nm. Genomes are linear, around 4-5.4kb in length. Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription, using the premature termination model of subgenomic RNA ...

Maize chlorotic mottle virus (MCMV) has become one of the most widespread corn viruses in the Hawaiian Islands. In the US Mainland, MCMV has been reported to be transmitted by chrysomelid beetles. However, none of these beetle species are established in Hawaii where the corn thrips, Frankliniella williamsi has been identified to be the main vector. In this research, I showed that thrips transmitted the virus with no evidence for latent periods. Both larvae and adults transmitted MCMV for up to 6 days after acquisition, with decreasing rates of transmission as time progressed. There was also no evidence that adult thrips that acquired the virus as larvae were competent vectors. Real-time RT-PCR assays showed that viral load was depleted from the vectors body after thrips had access to healthy plant tissue. Depletion of viral load was also observed when thrips matured from larvae to adults. Thrips were able to transmit MCMV after acquisition and inoculation access periods of 3 hours. In addition, ...

How is Feathery Mottle Virus (plant disease) abbreviated? FMV stands for Feathery Mottle Virus (plant disease). FMV is defined as Feathery Mottle Virus (plant disease) somewhat frequently.

Cowpea mottle virus ATCC ® PVAS-518™ Designation: Mokwa [Cowpea Mottle Carmovirus Antiserum] Application: Test animal used: rabbit Plant research

83062-04-8 - RNA (velvet tobacco mottle virus 2) - Searchable synonyms, formulas, resource links, and other chemical information.

Custom Spherical Mirrors - BMV Optical Technologies Inc. - Routinely manufactures both concave and convex spherical mirrors in virtually any material

Prune dwarf virus (PDV) is one of the members of Bromoviridae family, genus Ilarvirus. Host components that participate in the regulation of viral replication or cell-to-cell movement via plasmodesmata are still unknown. In contrast, viral infections caused by some other Bromoviridae members are well characterized. Bromoviridae can be distinguished based on localization of their replication process in infected cells, cell-to-cell movement mechanisms, and plant-specific response reactions. Depending upon the genus,

Pest/Path: Corn Lethal Necrosis (CLN) (Maize Chlorotic Mottle Virus (MCMV) and Wheat Streak Mosaic Virus or Maize Dwarf Mosaic Virus (MDMV ...

Pest/Path: Corn Lethal Necrosis (CLN) (Maize Chlorotic Mottle Virus (MCMV) and Wheat Streak Mosaic Virus or Maize Dwarf Mosaic Virus (MDMV ...

Maize Chlorotic Mottle Virus (MCMV) is a deleterious pathogen which causes Maize Lethal Necrosis Disease that result in substantial yield loss of Maize crop worldwide. The positive-sense RNA genome of MCMV (4.4 kb) encodes six proteins: P32 (32kDa protein), RNA dependent RNA polymerases (P50 and P111), P31 (31kDa protein), P7 (7kDa protein), coat protein (25 kDa). P31, P7 and coat protein are encoded from sgRNA1, located at the 3′end of the genome and sgRNA2, located at the extremity of the 3′genome end. The objective of this study is to locate the possible attachment sites of Zea mays derived miRNAs in the genome of MCMV, using four diverse efficient miRNA target prediction algorithms. In total, 321 mature miRNAs were retrieved from miRBase (miRNA database) and were tested for hybridization of MCMV. These algorithms considered the parameters of seed pairing, minimum free energy, target site accessibility, multiple target sites, pattern recognition and folding energy for attachment. Out of 321 only

watermelon silver mottle virus ATCC ® PVMC-55™ Designation: WS-Y NP Application: contains sequence nucleocapsid protein C polypeptide Plant research

Downy brome or cheat grass (Bromus tectorum L.) and field brome (B. arvensis L.; Synonym = Bromus japonicus Thunb. ex Murr.; Japanese brome) are two annual exotic species that have increased the intensity and frequency of fire cycles in the Intermountain West of the United States, with millions of dollars in associated costs (DiTomaso 2000). These invasive brome species have a different impact in the Northern Great Plains of North America where they commonly co-occur in disturbed sites (White and Currie 1983, Haferkamp et al. 1993). In these mixed-grass prairie rangelands, annual bromes compete against other forage species (Haferkamp et al. 1997) and reduce litter decomposition rates (Ogle et al. 2003), which negatively impacts ecosystem services of biomass production and soil nutrient availability. In central plains croplands, downy brome invades alfalfa fields (Kapusta and Strieker 1975), wheat fields (Wicks 1984, Ostlie and Howatt 2013), and perennial grass pastures and seed production areas (Wicks

PAN Czytelnia Czasopism, Phylogenetic Analysis of PDV Movement Protein Compared to Bromoviridae Members as Justification of Possible Intercellular Movement - Acta Biologica Cracoviensia s. Botanica

Scientists at the University of Wisconsin-Madison have captured strikingly improved images of the machinery positive-strand RNA viruses use to make copies of, or replicate, their RNA genomes.

Our office hours are from 9am-5:30pm EST Monday to Friday. If you would like us to call you during hours, send us your name and phone number we will gladly call you back. Or please call us back during office hours at 1-800-856-5685 ...

Bruce and Rachel Gunters Mk III Interim Commer 36619H, Haulin The Hume on 27/8/11. Bruce was one of the organisers of the run. (Andy Wright)

Non-radioactive biotinylated RNA probes specific for plus (+) and minus (-) sense RNAs of brome mosaic virus (BMV) were synthesized in vitro from a plasmid bearing a 200 base pair fragment complementary to the 3 terminus of each of the three genomic RNAs of the virus. Using virion RNAs isolated from BMV infected barley plants, the sensitivity of biotinylated RNAs as hybridization probes was compared with that of 32P-labeled probes in Northern hybridization assays. Although the sensitivity of biotinylated and 32P-labeled probes is similar (approximately 5 pg), the time required to detect the RNA bands was much less than for autoradiography; (-) sense RNAs could be detected in 30 min whereas 48 h or more were required by autoradiography. The value of biotinylated probes for following RNA stability was exemplified by the detection of supplied inocula in protoplasts 24 h post-inoculation. Quantitation of relative accumulation of progeny (+) and (-) sense RNAs by densitometry of the Northern blots ...

Rapid response to a food security threat. MLN is caused by the combination of the maize chlorotic mottle virus (MCMV) and other common cereal viruses mostly from the potyviridae family - a set of viruses that encompasses over 30 percent of known plant viruses - like the sugarcane mosaic virus (SCMV). This viral disease can result in up to 100 percent yield loss and has devastated the incomes and food security situation of many smallholder farmers in the region.. CIMMYT, in collaboration with national agricultural research institutions, national plant protection agencies and seed sector partners, developed a multi-layered response system including real-time intensive surveillance, screening, and fast-tracking of the MLN resistance breeding program. Thanks to the MLN Screening Facility in Naivasha, Kenya, maize breeders rapidly discovered that most popular maize varieties were susceptible, which could expose poor farmers to the risk of losing their entire maize crops.. Using its global collection ...

387440791 - EP 0832191 A4 2000-11-15 - RECOMBINANT VIRAL NUCLEIC ACIDS - [origin: WO9640867A1] The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systematically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates

Define mottle. mottle synonyms, mottle pronunciation, mottle translation, English dictionary definition of mottle. tr.v. mot·tled , mot·tling , mot·tles To mark with spots or blotches of different shades or colors. n. 1. A spot or blotch of color. 2. A variegated...

Optimization of an Elastic Network Augmented Coarse Grained Model to Study CCMV Capsid Deformation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.

Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the most common hereditary vascular dementia. CADASIL is a systemic disease of small and medium-sized arteries although the symptoms are almost exclusively neurological, including migraineous headache, recurrent ischemic episodes, cognitive impairment and, finally, subcortical dementia. CADASIL is caused by over 170 different mutations in the NOTCH3 gene, which encodes a receptor expressed in adults predominantly in the vascular smooth muscle cells. The function of NOTCH3 is not crucial for embryonic development but is needed after birth. NOTCH3 directs postnatal arterial maturation and helps to maintain arterial integrity. It is involved in regulation of vascular tone and in the wound healing of a vascular injury. In addition, NOTCH3 promotes cell survival by inducing expression of anti-apoptotic proteins. NOTCH3 is a membrane-spanning protein with a large extracellular domain (N3ECD) ...

Bromus inermis is a PERENNIAL growing to 1.2 m (4ft). It is hardy to zone (UK) 3. It is in flower in May, and the seeds ripen from August to September. The species is hermaphrodite (has both male and female organs) and is pollinated by Wind. Suitable for: light (sandy), medium (loamy) and heavy (clay) soils. Suitable pH: acid, neutral and basic (alkaline) soils. It can grow in semi-shade (light woodland) or no shade. It prefers dry or moist soil.

Summary:Delayed planting has been suggested to reduce density of the bean leaf beetle Cerotoma trifurcata (Förster), the principal vector of Bean pod mottle virus. Therefore, planting date was explored to determine if it might impact damage caused by the virus. Four planting dates, ranging from mid-March to mid-June, and two soybean Glycine max (L.) cultivars were examined for their effect on the relative damage caused by the virus in central Iowa for the years 2000-2002. Damage was assessed in terms of ...

Read Cactus mild mottle virus is a new cactus-infecting tobamovirus, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.

Eduardo C. Alfonso, MD, Professor and Chair of Ophthalmology serves as the Medical Director of the Ocular Microbiology Core. Darlene Miller, Research Associate Director is the Scientific Director and imitates and collaborates on research projects and studies. Jorge Maestre-Mesa, MD, PhD is our molecular biology expert and is instrumental in molecular studies on ocular microbiomes and individual microbes. Medical Technologists, Edith Perez, BS, MT (ASCP) and Martha Diaz, BS MT (ASCP-retiring), assist in maintaining the ocular microbiology databank.. ...

Lindenbach Lab is a Yale University research laboratory that studies the replication of hepatitis C virus (HCV) and related positive-strand RNA viruses, led by Brett Lindenbach.

Here are the steps to install the Pole Adapter.. The Pole Adapter kit consists of two parts: 1- the pole adapter and 2- the mid ring. First place your pole adapter onto a 1 inch pole.. Install the mid ring next:. 1.Loosen the wing nut at the bottom of the feeder (do not remove).. 2.Remove the cardinal ring.. 3.Remove every other perch.. 4.Install the mid ring.. 5.Place the perches back on.. 6.Place the cardinal ring back on.. 7.Extend opposing perches until they sit nicely in a seat.. 8.Tighten the bottom wing nut.. Now you are ready to install the feeder onto the pole adapter. ...

Codoñer, F.M., Cuevas, J.M., Sánchez-Navarro, J.A., Pallás, V. and Elena, S.F.(2005) Molecular Evolution of the Plant Virus Family Bromoviridae Based on RNA 3 Encoded Proteins. Journal of Molecular Evolution 61: 697-705. ...

Back in June 1973 the HCVC (as it then was) held a small rally in Peckham Rye Park, not far from Mr Banfields coach depot. A visiting fair was close by, and among the attractions was this mighty Albion of Irvins carrying the name Marvellous from one of their showmans engines