TY - THES. T1 - Chemical virology. T2 - decorating the interior of the cowpea chlorotic mottle virus. AU - Minten, Inge Jeannette. PY - 2011/4/4. Y1 - 2011/4/4. KW - METIS-283479. M3 - PhD Thesis - Research external, graduation external. SN - 978-90--9025926-0. PB - Radboud University Nijmegen. CY - Nijmegen. ER - ...
Positive-strand RNA viruses are the largest virus class and include many pathogens such as hepatitis C virus and the severe acute respiratory syndrome coronavirus (SARS). Brome mosaic virus (BMV) is a representative positive-strand RNA virus whose RNA replication, gene expression, and encapsidation have been reproduced in the yeast Saccharomyces cerevisiae. By using traditional yeast genetics, host genes have been identified that function in controlling BMV translation, selecting BMV RNAs as replication templates, activating the replication complex, maintaining a lipid composition required for membrane-associated RNA replication, and other steps. To more globally and systematically identify such host factors, we used engineered BMV derivatives to assay viral RNA replication in each strain of an ordered, genome-wide set of yeast single-gene deletion mutants. Each deletion strain was transformed to express BMV replicase proteins and a BMV RNA replication template with the capsid gene replaced by a ...
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Read "Remarkable variability of apple mosaic virus capsid protein gene after nucleotide position 141, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Under conditions of low ionic strength and a pH ranging between about 3.7 and 5.0,solutions of purified coat proteins of cowpea chlorotic mottle virus (CCMV) form sphericalmultishell structures in the absence of viral RNA. The outer surfaces of the shells in thesestructures are negatively charged, whereas the inner surfaces are positively charged due to a disordered cationic N-terminal domain of the capsid protein, the arginine-rich RNA-binding motif that protrudes into the interior. We show that the main forces stabilizing these .... ...
The genome is segmented and tripartite. The segments are distributive among 3 particle types of different sizes. The genome consists of three segments to four segments of linear, positive-sense, single-stranded RNA. The virion may contain minor species of non-genomic nucleic acid. Although the encapsidated nucleic acid is mainly of genomic origin, the virions may also contain subgenomic RNA. Subgenomic RNA is the mRNA (RNA-4, a subgenomic fragment of RNA-3) derived from genomic RNA-3. The complete genome is fully sequenced and is 8620-8800 nucleotides long. RNA-1 has been fully sequenced and 3158-3372-3900 nucleotides long; RNA-2 has been fully sequenced and 2799-3032-3400 nucleotides long; RNA-3 is sequenced but only an estimate is given and the sequence is 2117-2296-2600 nucleotides long; RNA-4 has been fully sequenced and is 800 nucleotides long. The genome has a base ratio of 24.4-25.95-28 % guanine; 24.9-26.05-27 % adenine; 19-20.62-22.2 % cytosine; 24-27.42-29 % uracil. The genome has a ...
The genome is segmented and tripartite. The segments are distributive among 3 particle types of different sizes. The genome consists of three segments to four segments of linear, positive-sense, single-stranded RNA. The virion may contain minor species of non-genomic nucleic acid. Although the encapsidated nucleic acid is mainly of genomic origin, the virions may also contain subgenomic RNA. Subgenomic RNA is the mRNA (RNA-4, a subgenomic fragment of RNA-3) derived from genomic RNA-3. The complete genome is fully sequenced and is 8620-8800 nucleotides long. RNA-1 has been fully sequenced and 3158-3372-3900 nucleotides long; RNA-2 has been fully sequenced and 2799-3032-3400 nucleotides long; RNA-3 is sequenced but only an estimate is given and the sequence is 2117-2296-2600 nucleotides long; RNA-4 has been fully sequenced and is 800 nucleotides long. The genome has a base ratio of 24.4-25.95-28 % guanine; 24.9-26.05-27 % adenine; 19-20.62-22.2 % cytosine; 24-27.42-29 % uracil. The genome has a ...
Harrison Brome, the 20-year old brooding crooner released his debut EP Fill Your Brains in July 2016. The EP featured Spotify & blog favorites "Sex Calls," "Boy," "Pools," "Midnight Island," "Gambling Hearts" and title track "Fill Your Brain". With the recent release of Body High EP, Bromes music is a powerful composition of work showcasing sophistication beyond his years. Its evident that the young Brome has been honing his craft; its poignant melodies work together with his harmonized howls for a hauntingly somber listening, evocative of the notoriously dark and gloomy weather of his hometown of Vancouver. This is just the beginning of the emotional roller coaster that is Harrison Brome.. ...
Principal Investigator:NOMURA Yasuyuki, Project Period (FY):1990 - 1991, Research Category:Grant-in-Aid for General Scientific Research (B), Research Field:Biological pharmacy
Brome mosaic virus (BMV) is a small (28 nm, 86S), positive-stranded, icosahedral RNA plant virus belonging to the genus Bromovirus, family Bromoviridae, in the alphavirus-like superfamily. BMV commonly infects Bromus inermis (see Bromus) and other grasses, can be found almost anywhere wheat is grown, and thrives in areas with heavy foot or machinery traffic. It is also one of the few grass viruses that infects dicotyledonous plants; however, it primarily infects monocotyledonous plants, such as barley and others in the family Gramineae. BMV was first isolated in 1942 from bromegrass (Bromus inermis), had its genomic organization determined by the 1970s, and was completely sequenced with commercially available clones by the 1980s. The alphavirus-like superfamily includes more than 250 plant and animal viruses including Tobacco mosaic virus, Semliki forest virus, Hepatitis E virus, Sindbis virus, and arboviruses (which cause certain types of encephalitis). Many of the positive-strand RNA viruses ...
Daniel, Marie-Christine and Tsvetkova, Irina B. and Quinkert, Zachary T. and Murali, Ayaluru and De, Mrinmoy and Rotello, Vincent M. and Kao, C. Cheng and Dragnea, Bogdan. (2010) Role of Surface Charge Density in Nanoparticle-Templated Assembly of Bromovirus Protein Cages. ACS Nano, 4 (7). p. 3853. ISSN 1936-0851 Aniagyei, Stella E. and Kennedy, Chelsea J. and Stein, Barry and Willits, Deborah A. and Douglas, Trevor and Young, Mark J. and De, Mrinmoy and Rotello, Vincent M. and Srisathiyanarayanan, D. and Kao, C. Cheng and Dragnea, Bogdan. (2009) Synergistic Effects of Mutations and Nanoparticle Templating in the Self-Assembly of Cowpea Chlorotic Mottle Virus Capsids. Nano Letters, 9 (1). pp. 393-398. ISSN 1530-6984 De, Mrinmoy and Rana, Subinoy and Akpinar, Handan and Miranda, Oscar R. and Arvizo, Rochelle R. and Bunz, Uwe H. F. and Rotello, Vincent M.. (2009) Sensing of proteins in human serum using conjugates of nanoparticles and green fluorescent protein. Nature Chemistry, 1 (6). p. 461. ...
Page contains details about doxorubicin-conjugated tobacco mosaic virus capsid protein mutant assemblies . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
In the summer of 2005, Giau was a REU intern in Dr. C. Kaos lab at Texas A&M University. Her project was titled, "Visualization of Fluorescent Brome Mosaic Virus RNA3 in an In-vivo Environment.". During my stay at Texas, I worked with a plant virus called Brome Mosaic Virus. In recent years, this virus has become a model system for positive strand RNA viruses.. The internship was a rewarding experience because it was my first time being exposed to scientific research on a larger scale. Within one lab there were at least 4-5 large scale projects going on. It was definately an "action-packed" environment for science majors.. Secondly, I was very amazed to see how these scientists were so dedicated to their work. The Post-doc that I worked under spent at least 12 hours a day in the lab during weekdays, and he was always in the lab on the weekends as well. Through this internship I found out that the lab is where I want to be in the future.. Giau graduated magna cum laude with a double major in ...
1I4B: Structural and thermodynamic studies on mutant RNA motifs that impair the specificity between a viral replicase and its promoter
By using the ability of the positive-strand RNA ((+)RNA) virus BMV to replicate in yeast it was previously shown that subunits of the LSm1-7 ring, as well as Pat1 and Dhh1 play an essential role in the transit of the BMV ...
POSTDOCTORAL POSITIONS IN VIRAL RNA REPLICATION, GENE EXPRESSION AND VIRUS-HOST INTERACTIONS Creative, highly motivated researchers are sought for postdoctoral positions studying the functions and interactions of viral and host factors in RNA replication, gene expression, and related events in positive-strand RNA viruses. Unique opportunities to identify and study relevant host factors are available through our demonstration that bromovirus and nodavirus RNA replicons can replicate and express foreign genes in yeast, allowing the use of powerful yeast genetics to identify pertinent host genes and to greatly facilitate other experiments (Janda and Ahlquist [1993] Cell 72:961-970; Quadt et al. [1995] PNAS 92:4892-4896; Price et al. (1996) PNAS 93:9465-9470). Bromoviruses and nodaviruses are well-studied viruses with excellent genetic and biochemical resources for in vivo and in vitro studies of fundamental replication mechanisms. Significant opportunities are available in several areas of ...
in fact, both the 1a and 2a genes of brome mosaic virus were expressed as , transgenes, and an RNA3 derivitave as a replication substrate. I was just , trying to make two points to the curious layperson: first, that it is possible , for a virus to cross kingdoms (beyond the bunyaviridae) and second, that it is , difficult, and is unlikely to occur without a good deal of human or evolutionary effort, , Cheers! Good answer...but as Terry Hanzlik pointed out, flock house nodavirus infects plants (done in your institution) AND insects, and can multiply in cells of a number of animal cell lines...so maybe some of the effort isnt needed! And its not only Bunyaviridae; also Reoviridae and Rhabdoviridae, and some other as yet unclassified animal-type viruses which multiply in pants [Freudian, that - mean PLANTS, of course!] and insects. And there is evidence accumulating to suggest certain Potyviridae multiply in plants and fungi, and someone in our institute is convinced a certain relatively obscure ...
Machlomovirus is a genus of plant viruses, in the family Tombusviridae. Plants serve as natural hosts. There is currently only one species in this genus: the type species Maize chlorotic mottle virus (MCMV), which causes significant losses in maize production worldwide. MCMV was first identified in the U.S. state of Kansas causing corn lethal necrosis, a severe disease that negatively affects all stages of development for maize plants. Group: ssRNA(+) Order: Unassigned Family: Tombusviridae Genus: Machlomovirus Maize chlorotic mottle virus Viruses in Machlomovirus are non-enveloped, with icosahedral and spherical geometries, and T=3 symmetry. The diameter is around 28-34 nm. Genomes are linear, around 4-5.4kb in length. Viral replication is cytoplasmic. Entry into the host cell is achieved by penetration into the host cell. Replication follows the positive stranded RNA virus replication model. Positive stranded RNA virus transcription, using the premature termination model of subgenomic RNA ...
Cowpea mottle virus ATCC ® PVAS-518™ Designation: Mokwa [Cowpea Mottle Carmovirus Antiserum] Application: Test animal used: rabbit Plant research
83062-04-8 - RNA (velvet tobacco mottle virus 2) - Searchable synonyms, formulas, resource links, and other chemical information.
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Prune dwarf virus (PDV) is one of the members of Bromoviridae family, genus Ilarvirus. Host components that participate in the regulation of viral replication or cell-to-cell movement via plasmodesmata are still unknown. In contrast, viral infections caused by some other Bromoviridae members are well characterized. Bromoviridae can be distinguished based on localization of their replication process in infected cells, cell-to-cell movement mechanisms, and plant-specific response reactions. Depending upon the genus,
Pest/Path: Corn Lethal Necrosis (CLN) (Maize Chlorotic Mottle Virus (MCMV) and Wheat Streak Mosaic Virus or Maize Dwarf Mosaic Virus (MDMV ...
Pest/Path: Corn Lethal Necrosis (CLN) (Maize Chlorotic Mottle Virus (MCMV) and Wheat Streak Mosaic Virus or Maize Dwarf Mosaic Virus (MDMV ...
Maize Chlorotic Mottle Virus (MCMV) is a deleterious pathogen which causes Maize Lethal Necrosis Disease that result in substantial yield loss of Maize crop worldwide. The positive-sense RNA genome of MCMV (4.4 kb) encodes six proteins: P32 (32kDa protein), RNA dependent RNA polymerases (P50 and P111), P31 (31kDa protein), P7 (7kDa protein), coat protein (25 kDa). P31, P7 and coat protein are encoded from sgRNA1, located at the 3′end of the genome and sgRNA2, located at the extremity of the 3′genome end. The objective of this study is to locate the possible attachment sites of Zea mays derived miRNAs in the genome of MCMV, using four diverse efficient miRNA target prediction algorithms. In total, 321 mature miRNAs were retrieved from miRBase (miRNA database) and were tested for hybridization of MCMV. These algorithms considered the parameters of seed pairing, minimum free energy, target site accessibility, multiple target sites, pattern recognition and folding energy for attachment. Out of 321 only
watermelon silver mottle virus ATCC ® PVMC-55™ Designation: WS-Y NP Application: contains sequence nucleocapsid protein C polypeptide Plant research
PAN Czytelnia Czasopism, Phylogenetic Analysis of PDV Movement Protein Compared to Bromoviridae Members as Justification of Possible Intercellular Movement - Acta Biologica Cracoviensia s. Botanica
Scientists at the University of Wisconsin-Madison have captured strikingly improved images of the machinery positive-strand RNA viruses use to make copies of, or replicate, their RNA genomes.
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387440791 - EP 0832191 A4 2000-11-15 - RECOMBINANT VIRAL NUCLEIC ACIDS - [origin: WO9640867A1] The present invention relates to a recombinant viral nucleic acid selected from a (+) sense, single stranded RNA virus possessing a native subgenomic promoter encoding for a first viral subgenomic promoter, a nucleic acid sequence that codes for a viral coat protein whose transcription is regulated by the first viral subgenomic promoter, a second viral subgenomic promoter and a second nucleic acid sequence whose transcription is regulated by the second viral subgenomic promoter. The first and second viral subgenomic promoters of the recombinant viral nucleic acid do not have homologous sequences relative to each other. The recombinant viral nucleic acid provides the particular advantage that it systematically transcribes the second nucleic acid in the host. Host organisms encompassed by the present invention include procaryotes and eucaryotes, particularly animals and plants. The present invention also relates
Optimization of an Elastic Network Augmented Coarse Grained Model to Study CCMV Capsid Deformation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Cerebral Autosomal Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) is the most common hereditary vascular dementia. CADASIL is a systemic disease of small and medium-sized arteries although the symptoms are almost exclusively neurological, including migraineous headache, recurrent ischemic episodes, cognitive impairment and, finally, subcortical dementia. CADASIL is caused by over 170 different mutations in the NOTCH3 gene, which encodes a receptor expressed in adults predominantly in the vascular smooth muscle cells. The function of NOTCH3 is not crucial for embryonic development but is needed after birth. NOTCH3 directs postnatal arterial maturation and helps to maintain arterial integrity. It is involved in regulation of vascular tone and in the wound healing of a vascular injury. In addition, NOTCH3 promotes cell survival by inducing expression of anti-apoptotic proteins. NOTCH3 is a membrane-spanning protein with a large extracellular domain (N3ECD) ...
Summary:Delayed planting has been suggested to reduce density of the bean leaf beetle Cerotoma trifurcata (Förster), the principal vector of Bean pod mottle virus. Therefore, planting date was explored to determine if it might impact damage caused by the virus. Four planting dates, ranging from mid-March to mid-June, and two soybean Glycine max (L.) cultivars were examined for their effect on the relative damage caused by the virus in central Iowa for the years 2000-2002. Damage was assessed in terms of ...
Read "Cactus mild mottle virus is a new cactus-infecting tobamovirus, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Eduardo C. Alfonso, MD, Professor and Chair of Ophthalmology serves as the Medical Director of the Ocular Microbiology Core. Darlene Miller, Research Associate Director is the Scientific Director and imitates and collaborates on research projects and studies. Jorge Maestre-Mesa, MD, PhD is our molecular biology expert and is instrumental in molecular studies on ocular microbiomes and individual microbes. Medical Technologists, Edith Perez, BS, MT (ASCP) and Martha Diaz, BS MT (ASCP-retiring), assist in maintaining the ocular microbiology databank.. ...
... is a Yale University research laboratory that studies the replication of hepatitis C virus (HCV) and related positive-strand RNA viruses, led by Brett Lindenbach.
Codoñer, F.M., Cuevas, J.M., Sánchez-Navarro, J.A., Pallás, V. and Elena, S.F.(2005) Molecular Evolution of the Plant Virus Family Bromoviridae Based on RNA 3 Encoded Proteins. Journal of Molecular Evolution 61: 697-705. ...
Abstract: Clinical, cytogenetic and molecular observations on a sterile stallion 2n=65, XXY and a sterile mare 2n=64, XY are reported. The XXY stallion was a pure case since all cells showed the same chromosome constitution. In the cells of mare XY, no SRY gene was found by both FISH- and molecular analyses. Both carriers show normal body conformation but were sterile because the stallion had no spermatozoa in the ejaculate, as revealed by microscope observation, and the mare showed the typical gonadal dysgenesis since both the uterus and ovaries were hypoplasic, as revealed by both rectal palpation and ultrasonic analysis. Although a mutation and/or deletion of SRY gene seems to be involved in the sex reversal, this syndrome is not yet fully understood. The possibility of other genes playing an important role in this syndrome and changes in the protein encoded by SRY are discussed.. ...
Abstract We describe the molecular identification of human tuberculosis (TB) from vertebral bone tissue samples from three different populations of ancient Egypt. The specimens wer..
ID JQ809577; SV 1; linear; genomic RNA; STD; VRL; 335 BP. XX AC JQ809577; XX DT 18-JUN-2012 (Rel. 113, Created) DT 18-JUN-2012 (Rel. 113, Last updated, Version 1) XX DE Apple stem grooving virus isolate BJ-ml movement protein gene, partial cds. XX KW . XX OS Apple stem grooving virus OC Viruses; ssRNA viruses; ssRNA positive-strand viruses, no DNA stage; OC Tymovirales; Betaflexiviridae; Trivirinae; Capillovirus. XX RN [1] RP 1-335 RA Hu G.J., Hong N., Wang G.P.; RT "Apple stem grooving virus isolates from Chinese pears"; RL Unpublished. XX RN [2] RP 1-335 RA Hu G.J., Hong N., Wang G.P.; RT ; RL Submitted (21-MAR-2012) to the INSDC. RL College of Plant Science and Technology, Huazhong Agricultural University, RL Shizishan No. 1, Wuhan, Hubei 430070, China XX DR MD5; cec29cc7e6c2cbde6eb9d8f732fc9c7b. XX FH Key Location/Qualifiers FH FT source 1..335 FT /organism="Apple stem grooving virus" FT /host="pear" FT /isolate="BJ-ml" FT /mol_type="genomic RNA" FT /country="China" FT ...
Bean pod mottle virus (BPMV) is a virus disease spread primarily by the bean leaf beetle, Cerotoma trifurcata. Seed transmission is al
GNH is a holistic and sustainable approach to development, which balances material and non-material values with the conviction that humans want to search for happiness
Pharmacological and molecular studies on the interaction of varenicline with different nicotinic acetylcholine receptor subtypes. Potential mechanism underlying partial agonism at human a4 2 and a3 4 subtypes ...
Eesti Teadusinfosüsteem koondab informatsiooni teadus- ja arendusasutuste, teadlaste, teadusprojektide ning erinevate teadustegevuste tulemuste kohta.
Over the longer term this particular set of economic variables may actually pull the country out of its decadeslong deflationary quagmire. Japan as a nation needs to spend again, build again and buy again. Up until now, there hasnt been the will or a really compelling reason to do so. Now, whether you call it divine intervention or simply the flip side of a bad set of circumstances, Japan has its mojo back. It may take a few years before all of the above unfolds, but I think we are on the cusp of dramatic change within this country. Remember, you heard it here first, folks. Bill Schmick is an independent investor with Berkshire Money Management. (See About for more information.) None of the information presented in any of these articles is intended to be and should not be construed as an endorsement of BMM or a solicitation to become a client of BMM. The reader should not assume that any strategies, or specific investments discussed are employed, bought, sold or held by BMM. Direct your ...