Detect BrdU also known as Bromodeoxyuridine with Anti-BrdU Antibody, clone BU-1 (Mouse Monoclonal Antibody), that has been demonstrated to work in ICC. Find MSDS or SDS, a COA, data sheets and more information.
PromoCells Cell Proliferation Assay Kit II (BrdU) detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as less as 50-100 proliferating cells. ...
Chinese hamster ovary cells were seeded in the absence or presence of the polyamine synthesis inhibitor 2-difluoromethylornithine (DFMO). At 1-4 days after seeding, the cells were labelled for 15-120 min with the thymidine analogue bromodeoxyuridine (BrdUrd) and they were then fixed directly after the labelling period. In addition, cells were labelled for 30 min and they were then allowed to progress in BrdUrd-free medium during a defined post-labelling time before fixation. An indirect immunofluorescence technique, using the monoclonal BrdUrd antibody and the intercalating stochiometric DNA stain, propidium iodide, was applied to enable quantification of cellular BrdUrd and DNA contents, respectively, by flow cytometry (FCM). By comparing the mean DNA content of BrdUrd-labelled cells to the mean DNA contents of G(1) and G(2) cells, a relative measure of the position of the BrdUrd-labelled cells was obtained (relative movement). Relative movement data, obtained from control and DFMO-treated ...
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DiviTum™ assay determines the enzymatic activity of TK in patient samples. During the assay procedure, thymidine is replaced by its synthetic analog bromodeoxyuridine (BrdU), which gets phosphorylated and then incorporated into a synthetic DNA strand fixated in each well of a 96-well ELISA immunosorbent titer-plate.. The extent of BrdU incorporation depends on the activity of TK present in the serum sample; the more TK activity in the sample, the more BrdU is incorporated into synthetic DNA strands in the titer-plate well. The synthetic BrdU is then detected with anti-BrdU specific antibodies using the well-known ELISA assay technique. The DiviTum™ technology amplifies the signal, enabling the assay to measure thymidine kinase activity with high sensitivity.. ...
Abstract The interaction of a nucleoside analogue bromodeoxyuridine (BrdU) with human serum albumin (HSA) was studied to investigate the binding phenomenon and analyse the protein conformation upon...
Mouse anti-BrdU antibody, clone Bu20a recognizes incorporated BrdU in a variety of cell types. It is suitable for use on tissue sections in double-labeling techniques.
Anti-BrdU monoclonal antibody is offered as a stand alone product, for use in labeling cells that have incorporated BrdU (cells incubated in the presence of BrdU will incorporate this thymidine analog into any DNA that is synthesized during that time). This antibody, from the PRB-1 clone ... ...
Monoclonal antibody against 5-bromo-2-deoxyuridine BrdU expressed by BrdU for use in FACS, FFPE, Immunofluorescence, Immunohistochemistry against All species labeled with BrdU
We have brought together our full range of anti-BrdU antibodies in one place, making it easy for you to find exactly what you need. Buy online today
TY - JOUR. T1 - Increased incorporation of 5-bromodeoxyuridine into the DNA of proliferating tissues in partially hepatectomised mice. AU - Hill, Bridget T.. AU - Augenlicht, Leonard H.. AU - Baserga, Renato. PY - 1973/12/1. Y1 - 1973/12/1. UR - http://www.scopus.com/inward/record.url?scp=0015897107&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015897107&partnerID=8YFLogxK. U2 - 10.1016/0014-5793(73)80482-X. DO - 10.1016/0014-5793(73)80482-X. M3 - Article. C2 - 4763337. AN - SCOPUS:0015897107. VL - 37. SP - 298. EP - 302. JO - FEBS Letters. JF - FEBS Letters. SN - 0014-5793. IS - 2. ER - ...
Return to Modified Bases Modifications 5-Bromo-deoxyuridine is a photoreactive halogenated base that can be incorporated into oligonucleotides to crosslink them to DNA, RNA or proteins with exposure to UV light. Crosslinking is maximally efficient with light at 308 nm.. ...
Mouse genotyping. Null and wild-type mice were obtained from heterozygous p27Kip1 breeding pairs (in a mixed genetic background of C57BL/6 and B6SJL) (Kiyokawa et al., 1996). The genotype was determined by PCR analysis of genomic tail DNA. Briefly, tails were digested in lysis buffer (100 mm NaCl, 10 mm Tris, pH 8.0, 0.5% SDS, 25 mm EDTA, 148 μg/ml proteinase K stock) for 14-18 hr at 55°C. Genomic DNA was isolated and amplified by PCR using the following primers: 5′-CGCCCCGACTGCATCTGCGTGTTCGAA-3′ and 5′-TCAAACGTGAGAGTGTCTAACGG-3′ directed against thep27Kip1 gene and 5′-AGGGCTTATGATTCTGAAAGTCG-3′ corresponding to the neo sequence. After a 35 cycle reaction (denaturation at 94°C for 45 sec, annealing at 62°C for 1 min, extension at 72°C for 1 min), the wild-type allele yielded a 210 bp PCR product and the null allele yielded a longer 290 bp product because of neo insertion in the first exon.. Whole-mount bromodeoxyuridine incorporation.Bromodeoxyuridine (BrdU, Sigma, St. Louis, ...
Buy our Pescadillo 293T transfected lysate (positive control). ab94282 has been validated in western blot. Abcam now offers a 12-month guarantee.
The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Listing a study does not mean it has been evaluated by the U.S. Federal Government. Read our disclaimer for details ...
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 550]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Mouse Monoclonal Anti-Bromodeoxyuridine/BrdU Antibody (BU20a) [DyLight 488]. Proliferation Marker. Validated: Flow, ICC/IF, IHC-Fr, IHC-P. Tested Reactivity: All Species. 100% Guaranteed.
Analysis of turnover of Treg by BrdU incorporation. BALB/c mice were treated with BrdU administered continuously for 7 d using osmotic pumps. Then, peripheral L
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hey its me ur hot food Not a bad idea, I recall our mouths can take exposure to much higher temperatures I grip it with my teeth and le...
Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
Under normal conditions, the adult zebrafish telencephalic VZ continuously supplies new neurons to the OB (Byrd and Brunjes, 2001; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We next performed a BrdU pulse-chase experiment to trace the migrating newly born progeny of ventricular proliferative cells from the telencephalic VZ to the injury site (Zupanc et al., 2005; Grandel et al., 2006; Adolf et al., 2006; Kishimoto et al., 2011). We followed the BrdU-labeled cells in three telencephalic regions - the VZ, the subpallium and the mdlPa (the region surrounding the injury site) - on 0, 3, 7, 10 and 14 dpl (Fig. 4A). At 0 dpl, a large number of BrdU-labeled cells was detected in the telencephalic VZ (n=5; Fig. 4B,B′,F). At 3 dpl, BrdU-labeled cells appeared in the subpallium and pallium along the pathway connecting the telencephalic VZ and the injury site in the injured hemisphere (n=5; Fig. 4C,C′,G). From 3 dpl onwards, the number of BrdU-labeled cells decreased in the ...
In the present study, we examined AQP4 function in reactive astrocytes compared between wild-type (WT) and AQP4-deficient (AQP4/KO) mice after a stab wound to the cerebral cortex. To examine activity of astrocytes, proliferating cells were labeled with 5-bromo-2-deoxyuridine (BrdU) incorporated in the drinking water provided to mice. By the immunofluorescent analysis using anti-BrdU antibody, astrocyte reactivity was at high level around the lesion site for WT mice 3 days after the stab wound to the brain, while it was much less for AQP4/KO mice. To identify the molecules related in injured mouse brain, we performed microarray analysis and found that more than 400 genes around the lesion site were upregulated 3 days after the wounding in WT mice ...
国内在庫あります!Biotin標識済みシープ・ポリクローナル抗体 ab2284 適用: IP,ELISA,IHC-FoFr,IHC-P,IHC-Fr,ICC/IF…BrdU抗体一覧 一次抗体にBiotinを直接標識し、操作時間の短縮と低いバックグラウンドを実現。
Bromodeoxyuridine (BrdU) (Proliferation Marker) Antibody - With BSA and Azide, Mouse Monoclonal Antibody [Clone BRD469 + BRD494 + BRD.3 ] validated in IHC-P, IF, FC (AH10977-20), Abgent
Now i have a new problem! This BrdU protocol needs Denaturation. Now i want to monitor GFP-transfected cells too. The HCl seems to denature the endogeneous GFP too. Is there a more acid resistable GFP? Perhaps an anti-GFP antibody? Or may i choose another method to denaturize the DNA for the anti-BrdU? Maybe temp.?Or stain for myc-tagged GFP ...
Monoclonal Anti-Bromodeoxyuridine (Clone BU6-4) is often used in cell cycle analysis studies; the level of bromodeoxyuridine (BrdU) incorporation into DNA is a direct parameter of cell number and growth. The monoclonal anti-bromodeoxyuridine antibody can be used in Western blot analysis under non-reducing and non-heating conditions and in flow cytometry applications. ...
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Because our examination of cell death did not reveal a loss of newly generated neurons in the cortical plate, we asked whether the reduced neuronal numbers in p107-deficient mice was caused by fewer neurons born at E13.5 (the time of BrdU injection). To address this question, we performed a 24-h BrdU incorporation to measure the rate of neuronal commitment. Pregnant dams were injected at E13.5, embryos were collected 24 h later at E14.5, and the number of strongly labeled BrdU-positive cells were counted (i.e., cells that underwent terminal mitosis at the time of injection). In addition, sections were double stained with PCNA to show that these cells are no longer cycling. Double labeling with BrdU and PCNA revealed that most BrdU-positive cells within the SVZ and IZ were no longer expressing PCNA, indicating that they were newly postmitotic (Fig. 8, d-f). Cell counts of this newly postmitotic population revealed a two-fold reduction in p107-deficient brains compared with wild-type controls ...
Mice exposed to the OIR model were injected with 0.1 mg/g 5-bromo-2-deoxyuridine or 5-ethynyl-2-deoxyuridine (EdU) dissolved in sterile water 21 and were humanely killed 2 hours after injection. For BrdU, the eyes were fixed in 4% paraformaldehyde (PFA) on ice for 3 minutes, then transferred to 70% ethanol and stored at −20°C for 2 hours. They were then washed in decreasing concentrations of ethanol for 10 minutes each, dissected in PBS, and washed for 30 minutes in 1 mL PBS/1% Triton X-100 on a horizontal shaker at low speed. The retinas were incubated at 37°C for 1 hour with 2 N HCl, washed in 1 mL 0.1 M sodium borate twice for 15 minutes, incubated overnight at 4°C with biotin-conjugated anti-BrdU antibody diluted 1:300 in PBS containing 1% BSA, and then washed three times with PBS-1% Triton X-100 for 5 minutes while being shaken at low speed. Retinas were incubated in Texas Red-labeled anti-biotin diluted 1:500 in PBS, 1% BSA for 2 hours with shaking while shielded from light, then ...
As the previously described method [4,22], 5-bromo-2-deoxyuridine (BrdU) immunohistochemistry was performed. The brain sections were treated with 0.5% Trioton X-100 in PBS for 20 minutes, treated with 50% formamide-2 x standard saline citrate at 65℃ for 2 hours, treated with 2 N HCl at 37℃ for 30 minutes, and then treated with 100mM sodium borate (pH, 8.5). The sections were treated with mouse monoclonal anti-BrdU antibody (1:600; Roche) during overnight at 4℃, treated with biotionylated mouse secondary antibody (1:200; Vector Laboratories, Burlingame, CA, USA) for 90 minutes, and treated with avidin-peroxidase complex (1:100; Vector Laboratories). To visualize, the section was treated with 50mM Tris-HC1 (pH, 7.6) in 0.02% DAB, 40-mg/mL nickel chloride, and 0.03% H2O2 during 5 minutes. With a mouse monoclonal anti-neuronal nucleic antibody (1:300; Chemicon International, Temecula, CA, USA), counter-staining was conducted upon the same sections. After the slides were dried under the room ...
Purpose: To examine the anatomical localization of limbal T cells relative to label-retaining cells (LRCs). Previously, we demonstrated the presence of presumed limbal regulatory (Treg) Foxp3-GFP+ T cells via in vivo epifluorescent imaging. In this study, we further characterize the localization of T cells in the limbus in conjunction with LRCs, i.e. presumed limbal stem cells, via immunofluorescence tomography.. Methods: H2B-GFP/K5tTA (HGK) mice express histone H2B-green fluorescent protein (GFP) under the control of the tetracycline and keratin 5 promoters, resulting in retention of GFP in slow-cycling LRCs after doxycycline administration. Following a 16 week pulse-chase period, the limbal region of HGK mice was fixed with 2% paraformaldehyde, sectioned and embedded in butyl-methyl-methacrylate, subjected to immunohistochemistry (IHC) with the T cell marker CD3, and imaged via immunofluorescence tomography to create high-resolution 3-D reconstructions of 200 sections. 3-D reconstructions were ...
Overall, our 2H2O labeling results are consistent with measurements of hippocampal neurogenesis by BrdU labeling. Assuming that the hippocampus has ≈2 × 106 total cells (Abusaad et al., 1999), the labeling rate of 0.2% per day in total C57Bl/6 hippocampus that we observed with 2H2O would be equivalent to ≈4000 labeled cells/day, a value comparable with estimates by 12- to 24-h saturation labeling with BrdU (Hayes and Nowakowski, 2002). Palmer et al. (1999) reported that 0.7% of progenitor cells were labeled after daily BrdU injection for 6 days in a confocal analysis of BrdU incorporation into gradient-isolated progenitor cells from rat hippocampus, a similar value as we measured by 2H2O labeling on similarly isolated rat hippocampal progenitors (1% per week). In mice, the hierarchy of baseline progenitor cell proliferation across different mouse strains in our study agreed well with strain effects on total hippocampal cell proliferation obtained by BrdU labeling (C57Bl/6 , BALB/c , ...
One of the major drawbacks of droplet sorting in a flow cytometer is the relatively low sorting speed. Thus, we have developed an alternative, faster sorting technique: photodamage cell sorting. In a photodamage cell sorter all unwanted cells, as detected with the first, measuring laser, are killed with the second, damaging laser. Thus, the cells need to be photosensitive to the second laser. In addition, a mechanism is needed to switch this laser on and off based on the sorting criteria. In our photodamage cell sorter, the ZAPPER, we use an acousto-optic crystal to switch the laser beam. Cells are made photosensitive by vital staining with photosensitizers. With cells grown in the presence of 5-bromo-2′-deoxyuridine (BrdUrd) and stained with Hoechst 33342 (H42) at least a 5-decade cell reduction is accomplished after irradiation with 400 mW UV light. With this system, sorting rates have been achieved of 30,000 cells per second. Due to the selection based on photodynamic killing, this sorting ...
Results: This study reports the eventual outcome of the 97 patients after 12 years. There were no significant associations between proliferation data of the index tumours and patient outcome. No adverse events were identified which could be attributed to the use of the halogenated pyrimidine label in vivo ...
Microfiche of typescript. [Urbana, Ill.]: Photographic Services, University of Illinois, U of I Library, [1989]. 2 microfiches (41 frames): negative ...
We have studied the effect of prenatal (late intrauterine) 5-bromo-2-deoxyuridine (BrdU) administration on the development of the GnRH pathway. BrdU is widely accepted as a tool for studying neurogenesis (pre and postnatal). On the other hand, this thymidine analogue proved to be effective influencing different developmental events too. Dams of C57Bl6 mice were treated on the last days of pregnancy (between E16, E17, E18, E19 and the time of birth, E20/21 days, respectively). The dams received daily injections of 15 ug/g body weight BrdU subcutaneously, dissolved in physiological saline, between 11 and 12 am. BrdU treatment during the last 3-4 days of pregnancy resulted in a significant decrease of the number of GnRH immunoreactive (ir.) neuronal cell bodies as well as ir. fibers. This decrease could be detected at all postnatal ages (P0/P1, P3/4, P6/8 and P21/23) studied. The higher cumulative doses of BrdU produced a more significant decrease of the GnRH ir. structures in comparison with the ...
symbol: BrUra; 5‐bromouracil; 5‐bromo‐2,4(1H,3H)‐pyrimidine‐dione; a synthetic analogue of thymine with mutagenic activity. It is incorporated into DNA as bromodeoxyuridine, which replaces thymidine and induces transitions of G‐C base‐pairing to A‐T ... ...
We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we fou …
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Several types of assays can be performed measuring galactosidase activity in yeast using 5-Bromo-4-chloro-3-indoxyl-α-D-fucopyranoside as subtrate.
chemBlink provides information about CAS # 160892-07-9, 5-Bromo-1,3-benzenedicarbonitrile, 5-Bromoisophthalonitrile, molecular formula: C8H3BrN2.
Kent. D. Dunlap, Anna C. Silva and Michael Chung. Environmental complexity, seasonality and brain cell proliferation in a weakly electric fish, Brachyhypopomus gauderio. J exp Biol 2011; 214, 794-805 Full Text IH. Pytte CL, Parent C, Wildstein S, Varghese C, Oberlander S. Deafening decreases neuronal incorporation in the zebra finch caudomedial nidopallium (NCM). Behav Brain Res. 2010 Aug 25;211(2):141-7 Full Text IH. Lassmann J, Sliwoski J, Chang A, Canning DA, Zderic SA. Deletion of One SERCA2 Allele Confers Protection Against Bladder Wall Hypertrophy in a Murine Model of Partial Bladder Outlet Obstruction. Am J Physiol Regul Integr Comp Physiol. 2008 Jan;R58-65 Full Text ...
TY - JOUR. T1 - Wound splinting modulates granulation tissue proliferation. AU - Carlson, Mark Alan. AU - Thompson, Jon S. PY - 2004/7/1. Y1 - 2004/7/1. N2 - Attachment of the extracellular matrix to a substratum is important for fibroblast survival and proliferation in three-dimensional in vitro culture systems. We hypothesized that wound matrix attachment in a wound splinting model would modulate wound cell proliferation in vivo. Male rats were excisionally wounded on the dorsum, and a splint was sutured to the wound edge. In one experiment (N=12), 6 rats were desplinted on day 5, and then all were sacrificed 24 h later, 6 h after 5-bromo-2′-deoxyuridine (BrdU) injection. In the second experiment (N=18), 6 rats each were desplinted, desplinted with wound edge release, or not disturbed, followed by BrdU injection and sacrifice 24 h later. BrdU-labeled nuclei were quantified on frozen sections of granulation tissue, cut at three different levels. In the first experiment, the percentage of ...
Subventricular Zone-Derived Neural Progenitor Cells Migrate Along a Blood Vessel Scaffold Toward the Post-Stroke Striatum Kojima T, Hirota Y, Ema M, Takahashi S, Miyoshi I, Okano H, Sawamoto K.. The subventricular zone (SVZ) of the adult brain contains neural stem cells that have the capacity to regenerate new neurons after various insults. Brain ischemia causes damage to brain tissue and induces neural regeneration together with angiogenesis. We previously reported that, after ischemic injury in mice, SVZ-derived neural progenitor cells (NPCs) migrate into the striatum, and these NPCs are frequently associated with blood vessels in the regenerating brain tissue. Here we studied the role of blood vessels during the neural regeneration in more detail. BrdU administration experiments revealed that newly generated NPCs were associated with both newly formed and pre-existing blood vessels in the ischemic striatum, suggesting that the angiogenic environment is not essential for the ...
This family represents the N-terminal region of Pescadillo. Pescadillo protein localises to distinct substructures of the interphase nucleus including nucleoli, the site of ribosome biogenesis. During mitosis pescadillo closely associates with the periphery of metaphase chromosomes and by late anaphase is associated with nucleolus-derived foci and prenucleolar bodies. Blastomeres in mouse embryos lacking pescadillo arrest at morula stages of development, the nucleoli fail to differentiate and accumulation of ribosomes is inhibited. It has been proposed that in mammalian cells pescadillo is essential for ribosome biogenesis and nucleologenesis and that disruption to its function results in cell cycle arrest [1]. This family is often found in conjunction with a PF00533 domain. ...
Stem cells of somatic tissues are hypothesized to protect themselves from mutation and cancer risk through a process of selective segregation of their template DNA strands during asymmetric division. Mouse mammary epithelium contains label-retaining epithelial cells that divide asymmetrically and retain their template DNA. Immunohistochemistry was used in murine mammary glands that had been labeled with [3H]thymidine during allometric growth to investigate the co-expression of DNA label retention and estrogen receptor (ER)-α or progesterone receptor (PR). Using the same methods, we investigated the co-localization of [3H]thymidine and ER-α or PR in mammary tissue from mice that had received treatment with estrogen, progesterone, and prolactin subsequent to a long chase period to identify label-retaining cells. Label-retaining epithelial cells (LRECs) comprised approximately 2.0% of the entire mammary epithelium. ER-α-positive and PR-positive cells represented about 30-40% of the LREC subpopulation.