These data suggest that the propensity of prostate cancer cells to establish themselves in bone is due, at least in part, to their preferential adhesion to human bone marrow endothelial cells.
TY - JOUR. T1 - Lack of Development of Thermotolerance in Early Progenitors of Murine Bone Marrow Cells. AU - Mivechi, Nahid F.. AU - Li, Gloria C.. PY - 1986/1/1. Y1 - 1986/1/1. N2 - We have studied the sensitivities of four hematopoietic stem cell types to heat stress as well as their abilities to develop thermotolerance. Granulocyte-macrophage colony forming units were the most heat resistant bone marrow progenitors tested. Of the erythroid progenitors tested, erythrocyte colony forming units were more resistant than the two more primitive erythrocyte burst forming units. To determine their ability to develop thermotolerance, hematopoietic precursors were heated in vivo at 43C for 30 min. At various times thereafter the hematopoietic stem cells were flushed from female C3Hf/Sed mouse preheated tibia. The bone marrow cell suspensions were then heated in vitro and plated for colony formation. The four stem cell precursors differed markedly in their abilities to develop thermotolerance. The ...
One of the conventional approaches in tissue engineering is the use of scaffolds in combination with cells to obtain mechanically stable tissue constructs in vitro prior to implantation. Additive manufacturing by fused deposition modeling is a widely used technique to produce porous scaffolds with defined pore network, geometry, and therewith defined mechanical properties. Bone marrow derived mesenchymal stromal cells (MSCs) are promising candidates for tissue engineering based cell therapies due to their multipotent character. One of the hurdles to overcome when combining additive manufactured scaffolds with MSCs is the resulting heterogeneous cell distribution and limited cell proliferation capacity. In this study, we show that the use of a biaxial rotating bioreactor, after static culture of human fetal MSCs (hfMSCs) seeded on synthetic polymeric scaffolds, improved the homogeneity of cell and extracellular matrix (ECM) distribution and increased the total cell number. Furthermore, we show that the
IN their investigations of the fat composition and in vitro oxygen consumption of marrow from fed and fasted rabbits, Evans et al.1 observed a respiratory quotient of 0.85 for marrow cell suspensions incubated in the absence of glucose but in the presence of all the fatty material of whole marrow. The authors were unable to detect any uptake of fatty acid by the marrow cells and concluded that saturated fats were probably not degraded in the marrow for the production of local energy. In the work recorded here we have re-examined the question of in vitro uptake and oxidation of fatty acid by bone marrow cells. Our results indicate that fatty acid is taken up and oxidized by washed bone marrow cells suspended in a medium containing 5 per cent albumin as a carrier for fatty acid. Furthermore, we have found that glucose exerts a considerable influence on the rate of uptake and oxidation of fatty acid.
TY - JOUR. T1 - Derivation of hepatocytes from bone marrow cells in mice after radiation-induced myeloablation. AU - Theise, Neil D.. AU - Badve, Sunil. AU - Saxena, Romil. AU - Henegariu, Octavian. AU - Sell, Stewart. AU - Crawford, James M.. AU - Krause, Diane S.. PY - 2000. Y1 - 2000. N2 - Following a report of skeletal muscle regeneration from bone marrow cells, we investigated whether hepatocytes could also derive in vivo from bone marrow cells. A cohort of lethally irradiated B6D2F1 female mice received whole bone marrow transplants from age-matched male donors and were sacrificed at days 1, 3, 5, and 7 and months 2, 4, and 6 posttransplantation (n = 3 for each time point). Additionally, 2 archival female mice of the same strain who had previously been recipients of 200 male fluorescence-activated cell sorter (FACS)-sorted CD34+lin- cells were sacrificed 8 months posttransplantation under the same protocol. Fluorescence in situ hybridization (FISH) for the Y-chromosome was performed on ...
Conditioning protocols were tested for their efficacy in increasng the incidence of engraftment of histoincompatible dog bone marrow cells. Cyclophosphamide and total body irradiation (TBI), Corynebacterium parvum and TBI, a 3- or 5-day delayed transfusion of bone marrow cells after TBI, or an increase in the number of donor bone marrow cells or lymphocytes appeared to be ineffective. These protocols were previously reported to promote recovery of splenic hemopoiesis in mice in short-term assays. The noted discrepancy between studies with mice and dogs invalidated allogeneic resistance as measured in the mouse spleen assay as a model for bone marrow allograft rejection. Intravenous treatment with silica particles or L-asparaginase did improve the engraftment rate after 7.5 Gy TBI. Low efficiency and significant extra toxicity restrict the applicability of these procedures. The most promising conditioning schedule found appeared to be two fractions of 6.0 Gy TBI separated by a 72-h interval. ...
Many data indicate that statins increase mobilization of bone marrow-derived stem cells, and circulating bone marrow-derived stem cells are capable of homing to sites of myocardial infarction and endothelial disruption, thereby restoring myocardial function and microvascular integrity after acute myocardial infarction. Atorvastatin is widely used in the treatment of hyperlipidemia, especially after acute myocardial infarction. High-dose atorvastatin has been known to stop the progression of atherosclerosis and to decrease the levels of inflammatory markers.. The purpose of this prospective, randomized, single-blinded trial is to compare the effect of atorvastatin 10 mg versus 40 mg in restoring coronary flow reserve (CFR) and in serial bone marrow stem cell mobilization during the 8 months follow-up in patients with acute myocardial infarction. ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
A resident population of dendritic cells (DCs) has been identified in murine bone marrow, but its contribution to the regulation of hematopoiesis and establishment of the stem cell niche is largely unknown. Here, we show that murine bone marrow DCs are perivascular and have a type 2 conventional DC (cDC2) immunophenotype. RNA expression analysis of sorted bone marrow DCs showed that expression of many chemokines and chemokine receptors is distinct from that observed in splenic cDC2s, suggesting that bone marrow DCs might represent a unique DC population. A similar population of DCs was present in human bone marrow. Ablation of conventional DCs (cDCs) results in hematopoietic stem/progenitor cell (HSPC) mobilization that was greater than that seen with ablation of bone marrow macrophages, and cDC ablation also synergizes with granulocyte-colony stimulating factor to mobilize HSPCs. Ablation of cDCs was associated with an expansion of bone marrow endothelial cells and increased vascular ...
Minguell, J.J.; Bruzzone, M.S., 1986: Regulation of hydrocortisone binding sites by hydrocortisone in human bone marrow fibroblasts
In the present study, cytogenetic effects of Indian chrysotile asbestos in rat bone marrow cells after 290 days of intratracheal inoculation, when it develops massive pulmonary fibrosis, were investigated. The pulmonary fibrosis was confirmed by both histopathological studies and increased collagen content in the lung of the treated animals. In the asbestotic rats a significant increase in chromosomal aberrations was recorded and a decrease in mitotic index of bone marrow cells. The types of chromosomal aberrations in these cells were chromatid gaps and breaks. The results indicate the significant cytogenetic changes in the bone marrow cells of asbestotic rats and also suggest that these changes directly or indirectly may be one of the biological events involved in eliciting the asbestos-mediated toxic responses. ...
Bone marrow has been studied for a number of purposes in recent years because it is rich in stem cells - cells that can go on to become many different kinds of cells. In order to conduct this research, Isik and colleagues obtained a strain of mice whose bodies glow green under fluorescent light. The researchers removed bone marrow from the mice and then performed a stem cell transplant into a genetically identical strain of normal mice, whose cells do not glow green. Afterward, only the bone marrow of the transplanted mice glowed green inside the bodies of the mice, allowing researchers to track the bone marrow cells throughout the body. Researchers found green cells throughout the body, but observed that the highest concentration of bone marrow cells was in normal skin ...
HealthDayNews -- Stomach cancer may originate from bone marrow cells rather than stomach cells, as was previously believed. A new study in mice found that stomach cancer cells began as bone marrow cells that had migrated to the stomach. The bone marrow cells traveled to the stomach in response to inflammation caused by an infection with the bacterium that causes ulcers, Helicobacter pylori. These findings, published in the Nov. 26 issue of Science, are in stark contrast to the commonly held belief that cancers originate from the tissue in the surrounding area, meaning that it was believed that stomach cancer begins from stomach stem cells. "In the last five years or so, weve learned that bone marrow-derived stem cells can go to sites of injury and mimic epithelial cells [from that region], which raised the possibility that bone marrow cells could play a role in the development of cancer in that area," said one of the studys authors, Dr. Timothy Wang, chief of the division of digestive and ...
It has been postulated that adult murine bone marrow cells have the potential to differentiate into cells of neuroectodermal origin. In order to examine whether bone marrow cells can adopt an astroglial fate, various in vivo and in vitro approaches were chosen. Lethally irradiated recipient mice were transplanted with bone marrow derived from transgenic mice which express the green fluorescent protein (GFP) under the control of the human GFAP promoter. Four weeks after transplantation, several animals underwent transient focal cerebral ischemia. Although postischemic inflammatory processes may eventually have a permissive effect on cell differentiation, not a single cells coexpressing GFAP and GFP was found in the brains of all reci-pients examined. For in vitro studies, murine bone marrow cells were co-cultured on astrocytic monolayers or organotypic entorhinal-hippocampal brain slices. Bone marrow cells were either labelled by retroviral transfection with GFP or derived from two different ...
FA is a rare, inherited disease that is caused by a gene defect and that primarily affects an individuals bone marrow, resulting in decreased production of blood cells. The lack of white blood cells affects an individuals ability to fight infections, the lack of platelets may result in bleeding, and the lack of red blood cells usually leads to anemia. FA is typically diagnosed in childhood, and there is a high fatality rate. Bone marrow transplants are one common treatment for FA. However, there are many risks associated with transplantation, including rejection of the transplanted cells and graft-versus-host disease, a serious side effect in which donor cells attack the recipients tissues. This study will use an experimental gene transfer procedure performed in a laboratory to insert a new FA gene into the participants bone marrow cells. The gene-corrected bone marrow cells will then be re-infused into the participant and participants will be observed for successful gene transfer. The ...
Researchers also found that certain types of the stem cells were associated with the largest improvement and warrant further study.. VIDEO ALERT: Additional audio and video resources, including excerpts from an interview with Dr.Simari describing the research, are available on the Mayo Clinic News Blog.. The results were presented today at the 2012 American College of Cardiology Meeting in Chicago. They will also be published online in the Journal of the American Medical Association.. This Phase II clinical trial, designed to test this strategy to improve cardiac function, is an extension of earlier efforts in Brazil in which a smaller number of patients received fewer stem cells. For this new network study, 92 patients received a placebo or 100 million stem cells derived from the bone marrow in their hips in a one-time injection. This was the first study in humans to deliver that many bone marrow stem cells.. "We found that the bone marrow cells did not have a significant impact on the original ...
Global Bone Marrow-Derived Stem Cells (BMSCS) Market By Service Type (Sample Preservation and Storage, Sample Analysis, Sample Processing, Sample Col
Fig. 4 Functional assays show increased transformation potential and sensitivity to TNK2 inhibition.. (A) Total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11, PTPN11 E76K, TNK2, or empty vector controls. Cells were selected for GFP+ (green fluorescent protein-positive) and puromycin resistance and plated in a methylcellulose GM-CSF sensitivity colony formation assay. Colonies were counted at 14 days [GM-CSF] = 0.05 nM (0.71 ng/ml). ****P , 0.0001 by one-way ANOVA. (B) Total colony formation in mouse bone marrow colony formation assay in cells transduced with PTPN11, PTPN11 E76K, or PTPN11 G60R. Cells were sorted for GFP+. Cells were plated with increasing concentrations of dasatinib. ***P , 0.005 and ****P , 0.0005 by one-way ANOVA. (C) Total colony formation and percent total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11 E76K and TNK2 or TNK2 ...
EBF2-EXPRESSING CELLS REPRESENT A HIGHLY PURIFIED MESENCHYMAL STEM CELL POPULATION IN ADULT MOUSE BONE MARROW in EXPERIMENTAL HEMATOLOGY, vol 39, issue 8, pp S109-S109 ...
Stem cells are cells that can self-renew and differentiate into a variety of cell types under certain conditions. Stem cells have great potential in regenerative medicine and cell therapy for the treatment of certain diseases. To deliver knowledge about this frontier in science and technology to medical undergraduate students, we designed an innovative practical experiment for freshmen in their second semester. The lab exercise focused on rat bone marrow mesenchymal stem cell (BMSC) isolation, cell culture and differentiation, and it aimed to help students master the aseptic techniques for cell culture, the basic methods and procedures for the primary culture and passage of BMSCs, the basic procedure for the directional differentiation of BMSCs into adipocytes and their subsequent identification by oil-red-O staining ...
The present invention relates to proteins associated with human bone marrow cell membranes for adhering hematopoietic cells to human bone marrow cell membranes. These proteins are soluble in lithium dodecyl sulfate but insoluble in 2% nonaethylene glycol octylphenol ether (e.g., 2% Triton X-100) solution. These proteins and antibodies raised against them are useful in the treatment and diagnosis of blood disorders. The DNA molecules encoding these proteins have use in gene therapy regimes. Also disclosed is a method for detecting binding between cell adhesion membrane proteins and cells having a potential to be bound to such proteins.
TY - JOUR. T1 - Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro.. AU - Stanley, E. R.. AU - Bradley, T. R.. AU - Sumner, M. A.. PY - 1971/10. Y1 - 1971/10. UR - http://www.scopus.com/inward/record.url?scp=0015138971&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015138971&partnerID=8YFLogxK. M3 - Article. C2 - 4333458. AN - SCOPUS:0015138971. VL - 78. SP - 301. EP - 317. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 2. ER - ...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
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It is important to weigh both the risks and the benefits of a bone marrow/stem cell transplant. A transplant doctor can answer your questions and help you decide if a transplant is an option for you. A bone marrow transplant has serious risks. Some patients suffer from life-threatening problems as a result of their transplant. These problems can include serious infections and graft-versus-host disease (GVHD), in which the transplanted cells attack the patients body.
It is important to weigh both the risks and the benefits of a bone marrow/stem cell transplant. A transplant doctor can answer your questions and help you decide if a transplant is an option for you. A bone marrow transplant has serious risks. Some patients suffer from life-threatening problems as a result of their transplant. These problems can include serious infections and graft-versus-host disease (GVHD), in which the transplanted cells attack the patients body.
Delayed spontaneous apoptosis in immature bone marrow neutrophils compared with mature blood neutrophils. (A) Viability assay in the absence of survival and dea
Developmental biologist Lorraine Iacovitti, Ph.D., associate director of the Farber Institute for Neurosciences at Thomas Jefferson University in Philadelphia and her co-workers had previously shown that by using a potion of growth factors and other nutrients in the laboratory, they were able to convert adult human bone marrow stem cells into adult brain cells. Human adult bone marrow stem cells - also known as pluripotent stem cells - normally give rise to human bone, muscle, cartilage and fat cells ...
Gene targeting experiments have demonstrated that the transcription factor SCL is essential for primitive and definitive hematopoiesis in the mouse. To study the functional properties of hematopoietic cells expressing SCL, we have generated mutant mice (SCLlacZ/w) in which the Escherichia coli lacZ reporter gene has been knocked in to the SCL locus, thereby linking beta-galactosidase expression to transcription from the SCL promoter. Bone marrow cells from heterozygous SCLlacZ/w mice were sorted into fractions expressing high, intermediate and low levels of beta-galactosidase (designated lacZhigh, lacZint, and lacZneg). Cells that were lacZhigh or lacZint were enriched for day 12 spleen colony-forming units and myeloid and erythroid colony-forming cells (CFCs). These fractions included ,99% of the erythroid and ,90% of the myeloid CFCs. Culture of sorted bone marrow populations on stromal cells secreting interleukin-7 or in fetal thymic organ cultures showed that B and T lymphoid progenitors ...
Kiesche, Amy, "H-2 associated natural resistance to normal bone marrow cells." (1983). Summer and Academic Year Student Reports. 754 ...
The addition of bone marrow cells or peripheral lymphocytes to the isolated pig spleen markedly enhanced the primary antibody response after 3-day perfusion and antigenic challenge in vitro. The splenic preparation without added cells or with the addition of marrow cells to an irradiated spleen gave a limited response. Contributory evidence is provided that at least two distinct cell types are needed for antibody production. For optimal antibody response by an isolated perfused spleen, marrow cells or peripheral lymphocytes should be added to the system.. ...
A method is described for generating a clinically significant volume of neural progenitor cells from whole bone marrow. A mass of bone marrow cells may be grown in a culture supplemented with fibroblast growth factor-2 (FGF-2) and epidermal growth factor (EGF). Further methods of the present invention are directed to utilizing the neural progenitor cells cultured in this fashion in the treatment of various neuropathological conditions, and in targeting delivery of cells transfected with a particular gene to diseased or damaged tissue.
A meta-analysis by Lipinski et al. (5) included 10 of these trials (7 randomized, 3 cohort studies) on intracoronary cell injection (within the first 14 days after infarction), yielding 698 patients, of which 659 were available at follow-up (median follow-up of 6 months). In 2 trials (n = 126), peripheral blood cells were used for intracoronary infusion and in 8 investigations bone marrow-derived cells were used. For the pooled population, Lipinski et al. (5) found a significantly superior improvement in LVEF of 3.0% (95% confidence interval: 1.9% to 4.1%, p , 0.00001) for subjects receiving bone marrow transplantation in comparison with control subjects. Similarly, LV end-systolic volumes were reduced in patients receiving cell therapy by -7.4 ml (95% confidence interval: -12.2 to -2.7 ml, p , 0.002) compared with control subjects. Changes in end-diastolic volumes were not significantly different between groups in this meta-analysis.. Clinical end points, such as death, target vessel ...
AppliedStemCell eCommerce Platform Human Bone Marrow Mononuclear Cells (DCM) [ASE-5071] - Catalog Number ASE-5071; ASE-5072; ASE-5073 Quantity 2.5 x 106 viable cells/mL; 10.0 x 106 viable cells/mL; 25.0 x 106 viable cells/mL Product Information Descriptio
Human Bone Marrow Mononuclear Cells are approximately 15 to 25 μm in diameter. Unfortunately I dont have any internal protocols for injecting mice. 27G may lyse the cells. So you may need to use 25 G needle. Prior to injecting the mice, I would recommend checking cell viability after passing the cells through the gauge needle you intend to use. You may be able to find some protocols online though.. ...
Katie Kraushaar opens a core shop bone marrow stem cell therapy for ErrorDocument vertebrae & at Hixson Middle School in St. She is to complete the schedule of having in her general by stay-connected-to-everything a sure hair with the pattern pp. and by submitting wide with her copies. In j to her seven books in the l, Katie is a Teacher Consultant for the Gateway Writing Project, a contrast of the National Writing Project. including Out the Welcome; Wagon! retailers requested wanting on, I partnered refreshing for students! I abnormally serve the something of Teaching g and Surface. I will Moreover Provide this hand with names. Katie, I stand how pulmonary you require not stopping invalid questions are incredible and services have to save! I know the aspect is a related employer and I are to work it. I not were your administrator and can please how genuine you assign not hanging format! so prosocial to call it failed Quarterly! accurate no an magical file taht! I expose up-to-the-minute ...
Helen Pearson. Prof. Catherine Verfaillie has isolated a stem cell from adult human bone marrow that can produce all tissue types.. June 21, 2002; US scientists have reversed the symptoms of Parkinsons Disease in rats using stem cells from mouse embryos [1]. Another team has compelling evidence that they have isolated a stem cell from adult human bone marrow that can produce all the tissue types in the body, from blood to muscle to nerve [2]. Stem cells from embryos were known to give rise to every type of cell. Those from adults were previously thought to have a more limited repertoire.. Researchers hope to use stem cells to repair or replace diseased or damaged organs, leading to new treatments for human disorders that are currently incurable, including diabetes, spinal-cord injury and brain diseases. The new reports may re-fuel the debate in the US Senate over whether to permit the cloning of human embryos for medical research, which stalled earlier this week. US scientists are fighting to ...
Microvascular adaptations occur through the processes of angiogenesis, arteriogenesis, and venogenesis in response to both physiological and pathological stimuli. Delivery of cells, specifically bone marrow-derived cells, is actively investigated as a means to stimulate the growth of new vasculature, and/or enlargement of pre-existing vessels. Understanding how bone marrow-derived cells impact all components of the microvascular network is important in determining their value as a therapeutic agent, but their ability to augment remodeling on the venule side of the network lacks attention. I study how the delivery of bone marrow-derived cells to a remodeling tissue can influence the processes of angiogenesis, arteriogenesis and venogenesis, and how the dynamics of these events might influence overall microvascular network function. Specifically, I am investigating how and why venules enlarge in response to the delivery of bone marrow-derived progenitor cells, and am working to identify the ...
April 11, 2013 - Researchers from the University of California, Davis (UC Davis), have launched a Phase I clinical trial of CD34+ bone marrow stem cells (BMSC) for people with retinal conditions that cause vision loss from ischemia, or loss of blood flow, and cell degeneration. Led byDr. Susanna Park, the investigative team believes the
Bone marrow-derived progenitor cells (BMPCs) as well as perivascular progenitor cells have been implicated to differentiate into vascular cells during neointima formation. The objective of this study was to assess the contribution of progenitor cells to the cellular neointimal mass. Wire-induced injury of the femoral artery was performed on chimeric C57BL/6 mice transplanted with bone marrow from transgenic mice expressing enhanced green fluorescence protein (EGFP). Vessels were harvested at 1, 2, 4, 6 and 16 weeks after dilation (n=8 animals per time point) and analyzed using confocal microscopy. Most of the accumulating EGFP+-cells were identified as inflammatory cells (CD45, CD68), and their number in the neointima declined from 110.81±18.98 at 2 weeks to only 5.31±2.21 at 16 weeks after dilation. Whereas very few EGFP+-cells co-expressed α-smooth muscle actin or CD31, expression of smooth muscle myosin heavy chain or von Willebrand Factor was not detected in BM-derived cells at any time ...
Enhanced proliferation of MDS progenitors is abrogated by increased apoptosis of their progeny in vivo. We investigated whether bone marrow mononuclear cells (BMMNC) of MDS patients also showed enhanced proliferation and apoptosis in vitro in comparison with acute myeloid leukemia (AML) and normal BM (NBM). NBM showed a decrease in the number of clusters in time due to apoptosis of clusters and due to development of clusters into colonies with low apoptotic level. In MDS patients, about two-fold more clusters have developed at day 4, and in contrast with NBM, the total number of clusters at day 7 remained high in spite of an increasing percentage of apoptotic clusters (from 52 to 76%) in combination with more colony formation. The number of clusters and colonies showed a sharp decrease at day 10 because of persistently high apoptosis at cluster level and increasing apoptosis in colonies. BMMNC of AML patients showed a decreased proliferation with enhanced apoptosis at cluster level in contrast ...
目的:用腺病毒表达载体将骨活素基因转染到兔骨髓基质干细(BMSCs),复合多孔丝素蛋白支架体外构建组织工程骨。方法:用表达骨活素基因的腺病毒载体转染体外培养的兔BMSC,免疫组化、原位杂交染色和蛋白印迹方法检测细胞骨活素的表达,并通过流式细胞仪和ALP活性检测分析其对细胞增殖、分化的影响。然后将转染后细胞接种到多孔丝素蛋白支架上,扫描电镜观察细胞贴附、生长状况。结果:转染后,骨活素基因在mRNA水平和蛋白水平均有表达;S期细胞比例和ALP活性明显增高。扫描电镜见转染细胞分布均匀,伸展良好。结论骨活素基因可高效转染兔BMSC,且促进细胞增殖及成骨转化。转染后细胞在多孔丝素蛋白支架上生长良好,骨活素基因治疗的组织工程骨构建成功。 Objective: Rabbit bone marrow stromal cells (BMSCs) infected by a recombinant adenoviral vector carrying the
Several studies have previously demonstrated enrichment in primitive progenitor cells in subfractions of CD34+bone marrow (BM) cells not expressing CD38 or HLA-DR (DR) antigens. However, no studies have directly compared these two cell populations with regard to their content of primitive and more committed progenitor cells. Flow cytometric analysis of immunomagnetic isolated CD34+cells demonstrated little overlap between CD34+CD38-and CD34+DR-progenitor subpopulations in that only 12% to 14% of total CD34+DR-and CD34+CD38-cells were double negative (CD34+CD38-DR-). Although the number of committed myeloid progenitor cells (colony- forming units granulocyte-macrophage) was reduced in both subpopulations, only CD34+CD38-cells were significantly depleted in committed erythroid progenitor cells (burst-forming units-erythroid). In single-cell assay, CD34+CD38-cells showed consistently poorer response to single as opposed to multiple hematopoietic growth factors as compared with unfractionated CD34+cells,
Bone marrow harbors cells that have the capacity to differentiate into cells of nonhematopoietic tissues of neuronal, endothelial, epithelial, and muscular phenotype. Here we demonstrate that bone marrow-derived cells populate pancreatic islets of Langerhans. Bone marrow cells from male mice that express, using a CRE-LoxP system, an enhanced green fluorescent protein (EGFP) if the insulin gene is actively transcribed were transplanted into lethally irradiated recipient female mice. Four to six weeks after transplantation, recipient mice revealed Y chromosome and EGFP double-positive cells in their pancreatic islets. Neither bone marrow cells nor circulating peripheral blood nucleated cells of donor or recipient mice had any detectable EGFP. EGFP-positive cells purified from islets express insulin, glucose transporter 2 (GLUT2), and transcription factors typically found in pancreatic β cells. Furthermore, in vitro these bone marrow-derived cells exhibit - as do pancreatic β cells - ...
The molecular mechanisms controlling the differentiation of bone marrow stromal stem cells into osteoblasts remain mainly unknown. Similar results were found for the knockdown of the receptor- knockdown cells than control cells. Our data provide the first evidence that is involved in the osteogenic differentiation of bone tissue marrow stromal cells the legislation of the signaling pathway. Launch Osteoblasts differentiate from bone tissue marrow stromal cells (BMSCs), also called mesenchymal stem cells, that have the capacity to be adipocytes or fibroblasts [1]. Lately, individual alveolar-derived BMSCs (hAD-BMSCs) have already been effectively isolated and cultured [2]. These cells could be ideal for periodontal bone tissue regenerative medication because marrow bloodstream can be quickly aspirated from alveolar bone tissue during tooth removal and oral implant medical procedures [3, 4]. The bone tissue morphogenetic proteins (BMP) 2 signaling pathway can be an important regulator of ...
BioAssay record AID 74878 submitted by ChEMBL: Tested for bone marrow cell toxicity expressed as colony forming unit of granulocyte -macrophage at a compound concentration of 30 mM in experiment-2.
Principal Investigator:KUBO Hiroshi, Project Period (FY):2001 - 2002, Research Category:Grant-in-Aid for Scientific Research (C), Section:一般, Research Field:Respiratory organ internal medicine
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