Retinoid agonist Am80-enhanced neutrophil bactericidal activity arising from granulopoiesis in vitro and in a neutropenic mouse model.
When an antibody-sensitized Escherichia coli B (parent strain) is treated with complement in the absence of lysozyme, free fatty acids (FFA) and lysophosphatidylethanolamine (LPE) are liberated into the lipid fraction of the surrounding medium in addition to a little amount of phosphatidylethanolamine (PE). However, when a phospholipase A-deficient mutant isolated from the parent strain is similarly treated, PE appears in the lipid fraction, but only traces of its degradation products are found. Therefore, most of the degradation of bacterial PE to FFA and LPE observed in the usual immune bactericidal reaction must be the result of the action of bacterial phospholipase A.. When liposomes which were made from a mixture of lipid fractions of sheep erythrocytes and of 14C-labeled E. coli are treated with complement, 14C-phospholipids are also liberated into the surrounding medium, but the degradation products (FFA and LPE) are not observed. Some of the liberated lipids seem to bind with some ...
Inspired by nature, CiMaas will bring two arms of the immune system together to enhance the killing capacity of the system as a whole.
Do you know that smoking is killing you both mentally and physically? Here are the reasons why you should quit smoking as early possible.
Definition of intracellular killing assay in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is intracellular killing assay? Meaning of intracellular killing assay as a finance term. What does intracellular killing assay mean in finance?
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Differences in molecular composition of lipopolysaccharides (LPS) between serum-sensitive (S) clinical isolates of Escherichia coli and serum-resistant (R) clones derived by serial passage in serum were demonstrated to determine sensitivity or resistance to killing by normal human serum (NHS). LPS from R clones had a greater proportion of higher-molecular-weight, more highly O-antigen-substituted subunits than LPS from their serum S parents. Utilization of a liposomal model with inserted LPS simulating bacterial cell walls established LPS as the site of serum bactericidal action. Liposomes containing S LPS were lysed, while liposomes containing R LPS were unaffected by NHS. R and S LPS were fractionated into higher (F1)- and lower (F2)-molecular-weight fractions. Liposomes containing R LPS or the F1 fraction of S and R LPS were not lysed by serum. Liposomes containing the F2 fraction of S or R LPS were lysed by serum analogous to that observed with liposomes containing intact S LPS. These ...
Disclosed are novel bactericidal/permeability-increasing (BPI) protein products wherein cysteine residue number 132 or 135 is replaced by another amino acid residue, preferably an alanine or serine residue and/or wherein the leucine residue at position 193 is the carboxy terminal residue. Also disclosed are DNA sequences encoding methods for the production of the same in appropriate host cells, and stable homogeneous pharmaceutical compositions containing the analogs suitable for use treatment of gram negative bacterial infection and its sequelae.
Forty strains of Aeromonas hydrophila and Aeromonas veronii recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (PHS). Based upon the results of this assay, two major populations could be defined. The first group (n = 20) consisted of serogroup 0:11 strains, all of which possessed a paracrystalline surface layer (S layer); all of these strains were refractory to the bactericidal activity of 65% PHS with the exception of A. hydrophila strain AH-121, which was composed of mixed subpopulations of serum-susceptible and serum-resistant clones. A second collection of isolates (n = 20), all of which were S-layer-negative, contained a subgroup of strains (n = 7) that were highly susceptible to complement-mediated lysis, showing a greater than 100-fold reduction of viable progeny within 30 min of exposure to 65% PHS. Serum-resistant strains from both groups could not be lysed by exposure of bacterial cells to polyclonal
Like the findings from Africa (6, 8), our results indicate the widespread occurrence of complement-dependent, antibody-mediated bactericidal activity against S. Typhimurium in sera from healthy adults in the Boston area, as well as an age-dependent increase in this activity during maturation from child to adult. However, in contrast to the results from Africa, the bactericidal antibodies in our study appear to be directed against S. Typhimurium LPS rather than outer membrane proteins. Our data also indicate that the bactericidal antibodies discriminate between LPS from S. Typhimurium and E. coli, suggesting that the antibodies recognize a determinant in the outer polysaccharide of LPS (22, 23). The bactericidal activities of 2 of our samples appeared to be affected by acetylation of the abequose residue in the LPS outer polysaccharide, suggesting fine differences in antigen specificity within our collection. IgG purified from our samples was sufficient to recapitulate the bactericidal activity ...
Bactericidal/permeability-increasing protein (BPI), a cationic protein isolated from human neutrophils, binds lipopolysaccharide (LPS), kills gram-negative bacteria, and neutralizes many of the effects of LPS in vitro and in vivo. We hypothesized that a recombinant 23-kDa NH2-terminal fragment of BPI (BPI23) would reduce acute lung injury in endotoxemic pigs. At -18 h, pigs received an intravenous priming dose of LPS (20 micrograms/kg). Anesthetized ventilated swine were randomized to receive 1) no further treatment (n = 4); 2) LPS (250 micrograms/kg over 50 min) and BPI23 (3-mg/kg bolus and 3 mg/kg over 60 min) (n = 6); or 3) LPS and thaumatin, a cationic protein devoid of LPS neutralizing activity that has a molecular mass and isoelectric point that are similar to that of BPI23 (n = 7). BPI23 treatment significantly ameliorated LPS-induced hypoxemia, functional upregulation of opsonin receptors on circulating phagocytes, and alveolitis but had no effect on the elaboration of tumor necrosis ...
BioAssay record AID 367413 submitted by ChEMBL: Bactericidal activity against Escherichia coli NIHJ JC-2 preincubated for 1 min before addition of growth medium measured after 40 to 48 hrs.
Overview of antimicrobial proteins called lysozymes Lysozymes, also known as muramidase, are the most important bactericidal proteins of the mucosal immunity. T
Normal兔Serum (Sterile)试剂datasheet (ab7487).Abcam抗体、ELISA、激动剂拮抗剂、表观遗传试剂、蛋白多肽,使用效果保证,中国70%以上现货。
Opsonic activity in sera from 27 military recruits vaccinated with the Norwegian meningococcal serogroup B outer membrane vesicle vaccine was measured as respiratory burst with polymorphonuclear leukocytes as the effector cells and meningococci of the epidemic strain as the target. The results were compared with antimeningococcal IgG antibodies against an outer membrane vesicle coat in an enzyme-linked immunosorbent assay and with serum bactericidal activity. The vaccinees were immunized twice, with a 6-week interval between the two. The serum samples studied were collected at day zero, after 6 weeks, and after 12 weeks. Both serum bactericidal activity and respiratory burst were measured by adding external serum as the complement source. The results revealed a significant increase in specific IgG response, serum bactericidal activity, and respiratory burst after vaccination. We found a highly significant correlation between the responses in all three assays (P , 0.0001). The highest correlation ...
It has been suggested that a hand rub should have at least activity against bacteria, yeasts and enveloped viruses [1]. In our study only the bactericidal activity of a hand gel based on 85% (w/w) ethanol was looked at. We were able to show for the first time that the tested gel kills the most relevant nosocomial bacterial pathogens in only 15 s. Ethanol is known to have a strong bactericidal activity with log10 reductions , 5 which has been demonstrated against some of the most common nosocomial pathogens such as Staphylococcus aureus and Pseudomonas aeruginosa [7, 19-22] and which includes activity against various mycobacteria [23-25]. Nevertheless, this type of comprehensive bactericidal activity as described in our study has so far only been demonstrated with a propanol-based hand rub and an exposure time of 30 s [8].. Suspension tests, however, are not the critical part in the assessment of the efficacy assessment of alcohol-based hand antiseptics [26]. In a study with tests under practical ...
Sialic acid in the lipolysaccharide of Haemophilus influenzae: Strain distribution, influence on serum resistance and structural characterization: Mol.Microbiol.
bactericidal activity under conditions of use. UNE-EN 1276: 2010 and 2011. Erratum Quantitative suspension test for the evaluation of bactericidal activity in the food, household and institutional area (phase 2, step 1). ENAC.
Normal Rabbit Serum, 5 ml. Serum is the clear, straw-colored, liquid portion of blood plasma that does not contain fibrinogen or blood cells and remains fluid after clotting.
1. Healthy adolescents and young adults treated with the Neisseria meningitidis vaccine MenB-FHbp had increased bactericidal antibody responses (hSBA titer)
Sunni Islamist Baluch militants have claimed responsibility for the November 6 killing of a prosecutor in Irans restive southeastern province of Sistan-Baluchistan.
Moreover the human α-Defensin human neutrophil peptide 1 was revealed to show anti-HIV activity. HNP-one inhibits the binding of the virus to its coreceptor , the endocytosis of the virus into the target cell as well as the release of the HIV-genome from the endosome into the cytoplasm. Even so HNP-one did not inhibit the endocytosis of Influenza A virus displaying some selectivity of the AMPs in their tropism. These final results evidently demonstrate that defensins not only screen antimicrobial activity but in addition are lively from viruses as nicely.Bactericidal/permeability-increasing protein belongs to the class of AMPs. In distinction to the over mentioned defensins BPI owing to the 55 kDa molecular measurement of the protein is structurally significantly a lot more intricate than the peptides, which are in the selection of 3-five kDa. The BPI protein loved ones contains of far more than ten associates but only BPI alone displays a powerful antimicrobial exercise. BPI functions ...
The immunological basis for resistance of certain strains of Neisseria gonorrhoeae to the bactericidal action of normal human serum was studied by investigating the potential role of factors which are known to interfere with each of the sequential steps that result in immune lysis of Gram-negative b …
Seham, M. Shash1, Ahmad, Z. Al-Herrawy2, Mohammed, M. Kamel2 and Hamdy, A.El-Sadik3 1 Department of Botany, Faculty of Science, Benha University, Benh...
Seham, M. Shash1, Ahmad, Z. Al-Herrawy2, Mohammed, M. Kamel2 and Hamdy, A.El-Sadik3 1 Department of Botany, Faculty of Science, Benha University, Benh...
Amox-Tabs is a broad-spectrum antibiotic which provides bactericidal activity against a wide range of common gram-positive and gram-negative pathogens. Amoxicillin has bactericidal activity against susceptible organisms similar to that of ampicillin.
Bactericidal effects of 8,9-dihydroxy-1,5,6,10b-tetrahydropyrrolo[2,1-a]isoquinolin- 3(2H)-one (Trolline) on selected entero-pathogenic bacteria
TY - JOUR UR - http://lib.ugent.be/catalog/pug01:131442 ID - pug01:131442 LA - eng TI - Decreased neutrophil bactericidal activity during phagocytosis of a slime-producing Staphylococcus aureus strain PY - 2000 JO - (2000) VETERINARY RESEARCH SN - 0928-4249 PB - 2000 AU - Barrio, Belén AU - Vangroenweghe, Fr DI08 801001347145 053711611855 AU - Dosogne, Hilde AU - Burvenich, Christian DI07 801000346934 0000-0002-1156-0981 AB - Phagocytosis and intracellular killing by bovine polymorphonuclear leukocytes (PMN) are important host defence mechanisms against mastitis caused by Staphylococcus aureus. We compared the phagocytosis and overall killing of a non slime-producing (NSP) S. aureus and its slime-producing (SP) variant by blood PMN, using an in vitro bacteriological assay. Seven clinically healthy Holstein-Friesian dairy cows in mid-lactation stage were used for this purpose. The percentages of overall killing for the NSP and SP variant were 34 +/- 3% and 21 +/- 4% (P < 0.05) and the ...
TY - JOUR. T1 - An apoptosis-differentiation program in human polymorphonuclear leukocytes facilitates resolution of inflammation. AU - Kobayashi, Scott D.. AU - Voyich, Jovanka M.. AU - Somerville, Greg A.. AU - Braughton, Kevin R.. AU - Malech, Harry L.. AU - Musser, James M.. AU - DeLeo, Frank R.. PY - 2003/2/1. Y1 - 2003/2/1. N2 - Human polymorphonuclear leukocytes (PMNs) are an essential part of innate immunity and contribute significantly to inflammation. Although much is understood about the inflammatory response, the molecular basis for termination of inflammation in humans is largely undefined. We used human oligonucleotide microarrays to identify genes differentially regulated during the onset of apoptosis occurring after PMN phagocytosis. Genes encoding proteins that regulate cell metabolism and vesicle trafficking comprised 198 (98 genes induced, 100 genes repressed) of 867 differentially expressed genes. We discovered that complex cellular pathways involving glutathione and ...
EN] The evaluation of animal welfare through innate immunity (Serum Bactericidal Activity - SBA, Hemolytic Complement Assay - HCA, lysozyme) and the antioxidant status of the body (Reactive Oxygen Substances - ROS and Antioxidant Power of plasma, AP) offers a reliable prognostic and diagnostic tool. The aim of the present study was to investigate trends and correlations between some traits of innate immunity and the oxidative status of fattening rabbits at different ages. Blood samples from 120 New Zealand White fattening rabbits at 45, 55, 65, and 75 d of age were collected and analyzed. The results showed that SBA did not have a normal distribution because of numerous 0 values. Data distribution was normal when only SBA , 0 values were considered. Lysozyme (mean value 27.19 microg/mL) and HCA (mean value 50.84 CH50% ) had stable trends at different ages and showed a tendency that was comparable to that obtained in other animal species. On the contrary, SBA (mean value 42.15%) showed an ...
I never really gave much thought to commercial cleaning products! they cleaned and thats what they were supposed to do… I knew that I was not supposed to get them in my eye or accidentally consume them of course! but other than that it had never occurred to me that they might be slowly killing…
Former motocross ace Mark Donnelly - recently acquitted of the one-punch killing of Monaghan teenager Jason McGovern - walked free from court yesterday when a judge gave him a suspended
MenBvac is an outer membrane vesicle vaccine against systemic meningococcal disease caused by serogroup B Neisseria meningitidis. In this placebo-controlled double-blind study including 374 healthy adolescents, the safety and immunogenicity of a schedule of three primary doses 6 weeks apart followed by a fourth dose a year later were evaluated. Antibody responses to the vaccine strain and heterologous strains (non-vaccine-type strains) and the persistence of these antibodies were measured by the serum bactericidal assay (SBA) and enzyme-linked immunosorbent assay up to 1 year after the last dose. The proportion of subjects with SBA titers of ≥4 against the vaccine strain increased from 3% prevaccination to 65% after the third dose. Ten months later, this proportion had declined to 28%. The fourth dose induced a booster response demonstrated by 93% of subjects achieving a titer of ≥4. One year after the booster dose, 64% still showed SBA titers of ≥4. Cross-reacting antibodies were induced ...
Klebsiella pneumoniae (KP) expressing hypermucoviscosity phenotype (HV-KP) has abundant capsular polysaccharide (CPS) and is capable of causing invasive syndrome. Sodium salicylate (SAL) reduces the production of CPS. The study was aimed to investigate the relationship between aspirin usage and KP-mediated invasive syndrome and the effect of SAL on HV-KP. Patients with community-acquired KP bacteraemia were prospectively enrolled. KP-M1, a serotype-K1 HV-KP clinical isolate, was used in the following experiments: CPS production, HV-KP phenotype, and the effect of SAL on neutrophils phagocytosis. The effect of oral aspirin intake on the leukocyte bactericidal activity was evaluated. Patients infected by HV-KP and diabetic patients with poor glycemic control were at an increased risk for invasive syndrome (p | 0.01); those who had recent use of aspirin (p = 0.02) were at a lower risk. CPS production was significantly reduced in the presence of SAL. The HV-KP phenotype and resistance to neutrophil
Background: Community-acquired pneumonia (CAP) is a common and potentially life-threatening infection. Innate immunity is the first line of defence, and antimicrobial peptides (AMPs) produced by white blood cells and at epithelial barriers participate by killing microorganisms and neutralizing bacterial toxins. We wanted to investigate whether concentrations of AMPs (1) are increased in CAP, (2) predict the clinical outcome, and (3) differ depending on the causative microbe. Methods: Plasma concentrations of AMPs were measured using an enzyme-linked immunosorbent assay in 89 patients with CAP, 21 patients with non-respiratory tract infections (non-RTI), and 63 healthy control subjects. Results: In subjects with CAP, mean plasma concentrations of secretory leukocyte protease inhibitor (SLPI) and bactericidal/permeability-increasing protein (BPI) were significantly higher than in healthy control subjects (85 vs 45 ng/ml, p , 0.001 and 48 vs 10 ng/ml, p , 0.001, respectively), but less markedly ...
Two Pfizer, Inc., studies released on Friday found that its bivalent rLP2086 recombinant vaccine candidate for meningococcal disease was able to generate bactericidal responses.
Immunogenicity was measured as the percentages of overall subjects with hSBA (human serum bactericidal assay) seroresponse, directed against Neisseria meningitidis (N meningitidis) serogroups A, C, W and Y, 28 days after one vaccination of MenACWY-CRM (day 29).. The seroresponse is defined as the percentages of subjects achieving hSBA ≥1:8 postvaccination with a prevaccination hSBA ,1:4 and the percentages of subjects achieving at least four-fold increases in postvaccination hSBA from day 1 in subjects with a baseline hSBA ≥1:4 ...
Percentage of participants with serum bactericidal activity (SBA) of the Meningococcal ACWY vaccine at one month after vaccination against A, C, W-135 and Y strains. Bactericidal activity (% ≥ 1:4, i.e., percentage of subjects with BCA titer ≥ 1:4; % ≥ 1:8, i.e. percentage of subjects with BCA titer ≥ 1:8) against a panel of genetically distinct meningococcal B strains:. ...
119 children with recurrent respiratory infections were investigated for immunocompetence. They were divided into two groups. The first, group A, comprised children who had had predominantly upper respiratory infections. Group B comprised those who had had repeated pneumonia in addition. The groups were comparable for gender and age (mean 4.2 years). All the children had reduced neutrophil chemotaxis, reduced neutrophil fungicidal capacity, and perhaps reduced T-cell percentages. Group B children had, in addition to the above, decreased neutrophil bactericidal capacity and a more marked depression of neutrophil chemotaxis. In both groups, some children had reduced immunoglobulin concentrations while others had either normal or increased concentrations. Serum complement, neutrophil iodination, and mitogen-induced lymphocyte transformation were comparable with adult controls in both groups.. ...
We assessed the bactericidal activity and mechanism of action of Revamil® source (RS) honey and manuka honey, the sources of two major medical-grade honeys. RS honey killed Bacillus subtilis, Escherichia coli and Pseudomonas aeruginosa within 2 hours, whereas manuka honey had such rapid activity only against B. subtilis ...
The recovery, structure and function of dog granulocytes were determined before and after freeze preservation. Leucocytes were isolated from defibrinated or anticoagulated whole blood and subsequent erythrocyte sedimentation on a column of 2:1 dextran (6%)-Isopaque (33.9%). Granulocytes isolated by these procedures were examined for changes in O2 consumption associated with phagocytosis, in vitro directed migration (chemotaxis), bactericidal activity, and ultrastructure before and after freezing. Granulocytes were frozen in DMSO (7.5%) and autologous serum of HBSS and 20% autologous serum at the rate of -1 C/min to -80 C and stored in liquid N2 vapor. It is concluded that dog granulocytes freeze preserved by these methods are functional in vitro, but that phagocytic, directed migration, and bactericidal functions and ultrastructure are impaired to different degrees, according to the method of isolation and preparation for storage. These results indicate the need for continued investigation on the
Definition of neutrophil microbicidal assay in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is neutrophil microbicidal assay? Meaning of neutrophil microbicidal assay as a legal term. What does neutrophil microbicidal assay mean in law?
Emergency medicine. The focus of adaptive changes in the respiratory system in its most General form can be reduced to finding the most perfect forms of regulation during muscular work. This search is completed with a high efficiency during low-intensity work, provide aerobic (oxygen) energy sources.. Respiration in terms of relative muscular rest in the vast majority of children of school age bears the cost of civilization" - prolonged sitting at a Desk, school Desk limits the mobility of the chest. Breathing becomes. shallow, shallow. Wellness, the value of such physiological respiration is small. The situation worsens when the child breathes through the mouth. The nose in this case is not fulfilling its bactericidal functions. The air is cleaned from dust particles, and at a low temperature and does not doubt. The subsequent chain of events completes the vicious circle: inflammatory processes in the nasopharynx doing breathing through KOs impossible or extremely difficult.. School-age ...
Lung tissue polymorphonuclear leukocyte (PMN) counts (expressed as PMNs/field) in 10 different fields, excluding airways and pulmonary vessels under 400 × magn
Amoxicillin is a semisynthetic antibiotic with a broad spectrum of activity. It provides bactericidal activity against a wide range of common gram-positive and gram-negative pathogens.
The full article is here at sciencemag.org A group led by engineer Xinpei Lu at the Huazhong University of Science and Technology in China believes it...
Discover Lifes page about the biology, natural history, ecology, identification and distribution of Discover Life -- BPI188783
Discover Lifes page about the biology, natural history, ecology, identification and distribution of Discover Life -- BPI446511
A Janesville man has been named as a person of interest in the killing of a Mazomanie woman, Dane County authorities said Wednesday.
A Janesville man has been named as a person of interest in the killing of a Mazomanie woman, Dane County authorities said Wednesday.