Comment on Biotinidase Deficiency (BTD)
Incidence - The incidence of profound biotinidase deficiency is approximately 1 in 137,000 births. The prevalence of partial biotinidase deficiency is approximately 1 in 110,000 people. Since partial biotinidase deficiency can be mild, it is possible that the true prevalence is more common.. The condition is most common among individuals of European descent. However, it is also reported among individuals of Turkish, Saudi Arabian, and Japanese descent.. The early-onset form usually begins during the newborn (neonatal) period. The juvenile form usually begins at about three months of age. Males and females are affected in equal numbers.. Inheritance This condition is inherited in an autosomal recessive pattern. The parents of an individual with biotinidase deficiency each carry one copy of the mutated gene, but they typically do not have any health problems associated with the condition.. Cause - Mutations in the BTD gene cause biotinidase deficiency. The BTD gene provides instructions for making ...
High incidence of profound biotinidase deficiency detected in newborn screening blood spots in the Somalian population in...
TY - JOUR. T1 - High incidence of profound biotinidase deficiency detected in newborn screening blood spots in the Somalian population in Minnesota. AU - Sarafoglou, K.. AU - Bentler, K.. AU - Gaviglio, A.. AU - Redlinger-Grosse, K.. AU - Anderson, C.. AU - McCann, M.. AU - Bloom, B.. AU - Babovic-Vuksanovic, D.. AU - Gavrilov, D.. AU - Berry, S. A.. PY - 2009/12. Y1 - 2009/12. N2 - Newborns identified with profound biotinidase deficiency (BTD) by the Minnesota Newborn Screening Program (MN NBS) between 1 October 2004 and 30 May 2008 were all from new immigrant groups. Thirty-three positive cases of BTD were identified out of 264 727 infants screened by the Wolf colorimetric system during the period of this study by MN NBS. Five cases of profound BTD (0.1 to ,0.6 nmol/min per ml) and 26 cases of partial BTD (0.9 to 2.3 nmol/min per ml) were later confirmed through measurement of serum biotinidase activity. The incidence of combined partial and profound BTD of 1/8540 and that of profound BTD of ...
Neonatal screening for profound biotinidase deficiency in the Netherlands: consequences and considerations | European Journal...
Biotinidase deficiency is a rare inherited metabolic disorder that can cause severe neurological symptoms. To prevent severe clinical presentations, it was included in the Dutch neonatal screening programme in 2007. Since then the number of cases detected has been high. This study set out to describe the incidence of the disease, the clinical and demographic characteristics of the neonates identified and the type of mutations found. In the south-western Netherlands, 304 982 neonates were screened between 2007 and 2012; and 92 were identified for further testing. Confirmatory testing revealed 6 (7%) with a profound biotinidase deficiency (|10% enzyme activity), 44 (48%) with a partial deficiency (10-30%) and 42 (46%) with normal activity (|30%). All six patients whose profound deficiency was confirmed had enzyme activities below 15% on neonatal screening. Mutation analysis was performed in 61 neonates: 5 profound, 35 partial and 21 normal. All five profound cases had two severe mutations.
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Biotinidase Deficiency: Background, Pathophysiology, Epidemiology
Biotinidase (BTD), a ubiquitous mammalian cell enzyme, is present in high levels in the serum, liver, and kidneys. Its primary enzymatic function is to cleave the vitamin biotin (also known as coenzyme R, vitamin H, or vitamin B7) from the organic compound biocytin.
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Kit Component:- KN204153G1, BTD gRNA vector 1 in pCas-Guide vector- KN204153G2, BTD gRNA vector 2 in pCas-Guide vector- KN204153D, donor vector…
Epigenetic regulation of chromatin structure and gene function by biotin<...
TY - JOUR. T1 - Epigenetic regulation of chromatin structure and gene function by biotin. AU - Hassan, Yousef I.. AU - Zempleni, Janos. PY - 2006/7/6. Y1 - 2006/7/6. N2 - Covalent modifications of histones are a crucial component of epigenetic events that regulate chromatin structures and gene function. Evidence exists that distinct lysine residues in histones are modified by covalent attachment of the vitamin biotin, catalyzed by biotinidase and holocarboxylase synthetase. Biotinylation of histones appears to be conserved across species. The following biotinylation sites were identified using both MS and enzymatic biotinylation of synthetic peptides: K9, K13, K125, K127, and K129 in histone H2A; K4, K9, and K18 in histone H3; and K8 and K12 in histone H4. Evidence was provided that biotinylated histone H4 is enriched in pericentromeric heterochromatin, and that biotinylation of histone H4 participates in gene silencing, mitotic condensation of chromatin, and the cellular response to DNA damage. ...
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This gene encodes a member of the vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The…
Aetiological investigations in early developmental impairment: are they worth it? | Archives of Disease in Childhood
Results 699 participants, 68.8% boys, median age at investigation 2 years 8 months (range 3 months to 11 years 5 months). 61 (8.7%) of participants had no investigations, and children with EDI∠were less likely to be investigated (χ2=12.5, p,0.05). A diagnosis was made in 166 children (23.7%) and was more frequent in EDI+ (EDI− 9.9%, EDI+ 27.3%, χ2=19.0; p,0.05). Full blood count, zinc protoporphyrin, renal or liver function, bone profile, biotinidase, creatine kinase or lead level revealed no diagnoses. The following investigations found causes for EDI: MRI (23.1%), microarray (11.5%), Fragile X (0.9%), plasma amino acids (1.2%), urine organic acids (0.9%) and thyroid function tests (0.5%). ...
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Biotinidase deficiency - Wikipedia
Biotinidase deficiency is an autosomal recessive metabolic disorder in which biotin is not released from proteins in the diet during digestion or from normal protein turnover in the cell. This situation results in biotin deficiency. Biotin, also called vitamin B7, is an important water-soluble nutrient that aids in the metabolism of fats, carbohydrates, and proteins. Biotin deficiency can result in behavioral disorders, lack of coordination, learning disabilities and seizures. Biotin supplementation can alleviate and sometimes totally stop such symptoms. Signs and symptoms of a biotinidase deficiency can appear several days after birth. These include seizures, hypotonia and muscle/limb weakness, ataxia, paresis, hearing loss, optic atrophy, skin rashes (including seborrheic dermatitis and psoriasis), and alopecia. If left untreated, the disorder can rapidly lead to coma and death. Biotinidase deficiency can also appear later in life. This is referred to as late-onset biotinidase deficiency. ...
Medical Home Portal - Biotinidase Deficiency
Diagnosis of partial and profound biotinidase deficiency
information. Symptoms may include seizures rash, ataxia, and developmental delay.
Prognosis is excellent with treatment. Untreated biotinidase deficiency can lead to
irreversible hearing and vision lost. Resources for children with biotinidase deficiency
are included.
Multiple Carboxylase Deficiency (Biotinidase deficiency)
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Differential gene expression during early development in brains of wildtype and biotinidase-deficient mice<...
TY - JOUR. T1 - Differential gene expression during early development in brains of wildtype and biotinidase-deficient mice. AU - Brigolin, Christian. AU - McKenty, Nathan. AU - Pindolia, Kirit. AU - Wolf, Barry. N1 - Funding Information: This work was funded by the Safra Research Fund at Henry Ford Hospital . We thank Dr. Alan Dombkowski at the Functional Genomics and Bioinformatics Core Facility at Wayne State University, Detroit, MI for his help with the analysis and helpful discussions. Publisher Copyright: © 2016 The Authors. PY - 2016/12/1. Y1 - 2016/12/1. N2 - Biotinidase deficiency is an autosomal recessively inherited disorder characterized by neurological and cutaneous abnormalities. Untreated individuals with biotinidase deficiency cannot recycle biotin from biocytin (N-biotinyl-ϵ-lysine), the proteolytic digestion product of protein-bound biotin. Biotin therapy can markedly resolve symptoms, or can prevent the development of symptoms if initiated early. To understand better the ...
Neonatal screening in Sweden and disease-causing variants in phenylketonuria, galactosaemia and biotinidase deficiency
Phenylketonuria (PKU) is caused by a defect in the conversion of the amino acid phenylalanine (Phe) to tyrosine (Tyr). Without treatment, patients develop mental retardation. Inclusion of the Phe/Tyr ratio has decreased the number of false positive screening outcomes to the present PPV of 0.92 without any known missed cases. The recall levels have been lowered several times since the start of screening. An increase in the incidence of patients with milder disease has been observed with time. We were able to show that the impact of the adjusted recall levels was low. Instead, milder genetic variants, which are more common in Southern Europe, are found more often, which is an effect of the large number of non-Nordic immigrants who have come to Sweden during the last 25 years. The immigration has widened the spectrum of detected pathogenic variants ...
Biotinidase deficiency should be considered in individuals thought to have multiple sclerosis and related disorders. - PubMed ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
2020 ICD-10-CM Diagnosis Code D81.810: Biotinidase deficiency
Free, official coding info for 2020 ICD-10-CM D81.810 - includes detailed rules, notes, synonyms, ICD-9-CM conversion, index and annotation crosswalks, DRG grouping and more.
Multiple Carboxylase Deficiency | Profiles RNS
A deficiency in the activities of biotin-dependent enzymes (propionyl-CoA carboxylase, methylcrotonyl-CoA carboxylase, and PYRUVATE CARBOXYLASE) due to one of two defects in BIOTIN metabolism. The neonatal form is due to HOLOCARBOXYLASE SYNTHETASE DEFICIENCY. The late-onset form is due to BIOTINIDASE DEFICIENCY ...
Imperial College Healthcare - Clinical Biochemistry
Symptoms of untreated biotinidase deficiency may appear at any time from 1 week to 10 years of age. The most common early symptoms include seizure activity of various types (myoclonic, grand mal, and focal or infantile spasms) and hypotonia. Other early symptoms include breathing problems (tachypnoea, hyperventilation, stridor and apnoea), skin rashes and alopecia. Later developmental delay, speech problems, ataxia, and vision and hearing problems may occur. Less frequent findings include feeding difficulties, vomiting/diarrhoea, fungal infections, hepatomegaly and splenomegaly. ...
RCPA - Organic acidaemias
A possible genetic basis should be considered in children with metabolic acidosis not attributable to another disorder, especially if the acidosis cannot be explained by elevated lactate and/or ß-hydroxybutyrate.. Blood gas arterial or venous.. Organic acids urine (collected during acidotic episode); a normal result, when not acutely ill, does not exclude these disorders. In some diseases (eg, biotinidase deficiency) metabolic acidosis may not occur, but the abnormal organic acids can be detected in the urine.. Identification of the specific disorder depends on the pattern of organic acids excreted during attacks.. Ammonia and Glucose should be measured, as many organic acidaemias are associated with hyperammonaemia and/or hypoglycaemia.. See also Hyperammonaemia, Hypoglycaemia. Naming of specific disorders is usually based on the acid(s) excreted or the deficient enzyme(s).. ...
SEPARATION DEVICES AND METHOD FOR SEPARATING PHOSPHORYLATED PEPTIDES AND PROTEINS - Patent application
TABLE-US-00004 TABLE 2 Fraction (4, 5, 6) Swissprot Protein Name Accession # ITIH4_HUMAN (Q14624) Inter-alpha-trypsin inhibitor heavy chain H4 precursor (ITI heavy chain H4) (Inter-alpha-i ITIH4_HUMAN (Q14624) Inter-alpha-trypsin inhibitor heavy chain H4 precursor (ITI heavy chain H4) (Inter-alpha-i PHL1_HUMAN (P80108) Phosphatidylinositol-glycan-specific phospholipase D 1 precursor (EC 3.1.4.50) (PI-G PLD) BTD_HUMAN (P43251) Biotinidase precursor (EC 3.5.1.12) KAIN_HUMAN (P29622) Kallistatin precursor (Serpin A4) (Kallikrein inhibitor) (Protease inhibitor 4) ZA2G_HUMAN (P25311) Zinc-alpha-2-glycoprotein precursor (Zn-alpha-2-glycoprotein) (Zn-alpha-2-GP) A1AG2_HUMAN (P19652) Alpha-1-acid glycoprotein 2 precursor (AGP 2) (Orosomucoid-2) (OMD 2) IBP3_HUMAN (P17936) Insulin-like growth factor binding protein 3 precursor (IGFBP-3) (IBP-3) (IGF-binding prot CO6_HUMAN (P13671) Complement component C6 precursor CLUS_HUMAN (P10909) Clusterin precursor (Complement-associated protein SP-40,40) ...
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Human Histone H3 (di methyl K36) peptide (ab1784) | アブカム
Human Histone H3 (di methyl K36) peptide Peptide datasheet (ab1784). Abcam offers quality products including antibodies, assays and other reagents.
Human Histone H3 (tri methyl K36) peptide (ab1785)
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Human Histone H4 (tri methyl K20) peptide (ab17567)
Buy our Human Histone H4 (tri methyl K20) peptide. Ab17567 is a blocking peptide for ab9053 and has been validated in BL. Abcam provides free protocols, tips…
Human Histone H3 (mono methyl K4) peptide (ab1340)
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Makita BL1430, BL1415, JT6226, LGG1230, LGG1430, MAK1430Li, MET1821 14.4V 3000mAh tööriistaaku Li-ion | PATAREID.EE
Fits for: MAKITA: BBO140, BCF050, BCL140, BCL140Z, BCL142, BCL142Z, BDA340, BDA340RFE, BDA340Z, BDA341, BDA341RFE, BDA341Z, BDF343, BDF343446RFJ, BDF343RHEX, BDF343RHEX4, BDF343RHEX5, BDF343RHJ, BDF440, BDF440SFE, BDF440Z, BDF441, BDF441RFE, BDF441SFE, BDF441Z, BDF442, BDF442RFE, BDF444RFE, BDF444Z, BDF446RFE, BDF446Z, BDF448, BDF448RFE, BFL201RZ, BFL301RZ, BFL402RZ, BFR440, BFR440RFE, BFR440SFE, BFR540, BFR540RFE, BFR540Z, BFS440, BFS440RFE, BFS441RFE, BFS441Z, BFT041RZ, BFT082RZ, BFT124RZ, BGA450RFE, BGA450Z, BHP343Z, BHP440, BHP440SFE, BHP441, BHP441RFE, BHP441SFE, BHP441Z, BHP442RF, BHP444RFE, BHP444Z, BHP446RFE, BHP446Z, BHR162, BHR162RFE, BHR162SFE, BHR162Z, BJR141, BJR141Z, BJV140, BJV140RF, BJV140RFE, BJV140Z, BML145, BMR100, BNJ160, BPT350RFE, BPT350Z, BSS500RFE, BSS500Z, BST110, BST110RFE, BST110Z, BST220, BST220RFE, BTD130, BTD130F, BTD130FRFE, BTD130FSFE, BTD130FSFER, BTD130FSFEW, BTD130FW, BTD130FZ, BTD130SFE, BTD132RFE, BTD133RFE, BTD133Z, BTD134D, BTD134RFE, BTD134Z, BTL060RFE, ...
Human Histone H2B type 1-D (HIST1H2BD) ELISA Kit
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4 Tips About Newborn Screening Tests
For more information about newborn screening tests, check out Newborn Screening Tests from KidsHealth.. The following is a list from the National Newborn Screening and Genetic Resource Center of the standard newborn screening procedures by state. For a pdf of this list, click here.. Newborn Screener: Alabama. Infants must be screened for:. Phenylketonuria (PKU), Congenital hypothyroidism, Galactosemia, Sickle cell disease, Congenital adrenal hyperplasia. Newborn Screener: Alaska Infants must be screened for:. Phenylketonuria (PKU), Congenital hypothyroidism, Galactosemia, Maple syrup urine disease, Biotinidase deficiency, Congenital adrenal hyperplasia. Newborn Screener: Arizona Infants must be screened for:. Phenylketonuria (PKU), Congenital hypothyroidism, Galactosemia, Maple syrup urine disease, Homocystinuria, Biotinidase deficiency, Sickle cell disease. Newborn Screener: Arkansas Infants must be screened for:. Phenylketonuria (PKU), Congenital hypothyroidism, Galactosemia, Sickle cell ...
SYSTEM AND METHODS FOR PROCESSING ANALYTE SENSOR DATA - Patent application
0248] The term analyte, as used herein, is a broad term and is used in its ordinary sense, including, without limitation, to refer to a substance or chemical constituent in a biological fluid (for example, blood, interstitial fluid, cerebral spinal fluid, lymph fluid or urine) that can be analyzed. Analytes may include naturally occurring substances, artificial substances, metabolites, and/or reaction products. In some embodiments, the analyte for measurement by the sensor heads, devices, and methods is analyte. However, other analytes are contemplated as well, including but not limited to acarboxyprothrombin; acylcarnitine; adenine phosphoribosyl transferase; adenosine deaminase; albumin; alpha-fetoprotein; amino acid profiles (arginine (Krebs cycle), histidine/urocanic acid, homocysteine, phenylalanine/tyrosine, tryptophan); andrenostenedione; antipyrine; arabinitol enantiomers; arginase; benzoylecgonine (cocaine); biotinidase; biopterin; c-reactive protein; carnitine; carnosinase; CD4; ...
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Studies of Disorders With Increased Susceptibility to Fungal Infections - Full Text View - ClinicalTrials.gov
This study is designed for the evaluation, diagnosis, and long-term follow up of selected patients with primary immune deficiencies and other conditions associated with fungal, and more specifically with Candida spp. infections. The primary immune deficiencies to be studied include, but are not limited to, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED), chronic mucocutaneous candidiasis (CMC), myeloperoxidase deficiency (MPO), immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX), Job s syndrome, chronic granulomatous disease (CGD), and biotinidase deficiency. Diabetic patients and infants also show increased susceptibility to such infections and might be studied. Patient participants (who we will refer to as patients in this study) will undergo evaluations that include history/physical, blood sampling, genetic testing, and possible tissue sampling. We may use some of the blood cells to investigate the utility of induced pluripotent stem cells (iPS) for ...
Studies of Disorders With Increased Susceptibility to Fungal Infections - Full Text View - ClinicalTrials.gov
This study is designed for the evaluation, diagnosis, and long-term follow up of selected patients with primary immune deficiencies and other conditions associated with fungal, and more specifically with Candida spp. infections. The primary immune deficiencies to be studied include, but are not limited to, autoimmune polyendocrinopathy candidiasis ectodermal dystrophy (APECED), chronic mucocutaneous candidiasis (CMC), myeloperoxidase deficiency (MPO), immune dysregulation polyendocrinopathy enteropathy X-linked (IPEX), Job s syndrome, chronic granulomatous disease (CGD), and biotinidase deficiency. Diabetic patients and infants also show increased susceptibility to such infections and might be studied. Patient participants (who we will refer to as patients in this study) will undergo evaluations that include history/physical, blood sampling, genetic testing, and possible tissue sampling. We may use some of the blood cells to investigate the utility of induced pluripotent stem cells (iPS) for ...
Clinical Trial: Interference Of Biotin Supplementation In Biotin-Streptavidin Platforms For Hormone Testing - Healthcare /...
U.S., Feb. 27 -- ClinicalTrials.gov registry received information related to the study (NCT03034707) titled Interference of Biotin Supplementation in Biotin-streptavidin Platforms for Hormone Testing on Jan. 17. Brief Summary: The B vitamin biotin is widely available as an over the counter supplement, often advertised and used to promote health of hair, skin and nails. Commercially available over the counter biotin supplements contain dose ranges up to 10 mg/day (ie 333 times higher than the recommended dietary allowance). The biotin molecule is also sometimes used as part of the lab technology to measure hormone and protein levels in the blood. It is possible that high doses of ingested biotin may interfere with accurate hormone or protein measurement using biotin related in vitro measurement systems. Such interference, if present, could lead to misdiagnosis. The study will analyze laboratory levels obtained with streptavidin-biotin assay systems while ingesting biotin in currently available ...
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Streptavidin, recombinant
Fluorescent Dyes , Biotins and Streptavidins , Streptavidin, recombinant; Streptavidin is a nonglycosylated, tetrameric protein, with each subunit able to bind a single molecule of the vitamin biotin. Streptavidin-biotin bond is the strongest known non-covalent interaction with Kd ~10-15 M. Because streptavidin lacks any carbohydrate modification and has a near-neutral pI, it has the advantage of much lower nonspecific binding than avidin. Streptavidin is broadly used in various applications such as immunoassays, histochemistry, FISH (Fluorescence In Situ Hybridization), flow cytometry, microarrays and blot analysis.
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Description Synthesized phospho histone Ser28 peptide derived from residues 1-100 of human histone H3. Purity gt;90% by HPLC analysis Concentration 0.3 mg/ml (100 micro;M) Formulation Tris-buffered solution Storage -20deg;C,...
BTD and beyond? - Print Topic - Archive
Despite regular organ function tests at our Mds laboratory being clear, the scan showed weak Adrenals and Liver weakness due to the Leaky Gut problems. I believe the Liver is responsible for the periodic vision distortion problems I have been experiencing for as long as I can remember. I was a very jaundiced colicy baby according to my late mother. Pieces of the jigsaw of problems I have had through life with food are now starting to fall into place and thanks to the lead given by the BTD I think I am going to make some more progress ...
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Biotin Supplements Strengthen Weak Nails - Health - NAILS Magazine
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Molecular Expressions Microscopy Primer: Specialized Microscopy Techniques - Hoffman Modulation Contrast Photomicrography...
This page illustrates crystallites of the important vitamin biotin (vitamin H) viewed under a combination of crossed polarization and Hoffman modulation contrast illumination.
Biotinylated Phospho Histone Ser 10 Peptide | EpiGentek
Description Synthesized phospho histone ser10 peptide derived from residues 1-100 of human histone H3, biotin conjugated. Purity gt;90% by HPLC analysis Concentration 0.28 mg/ml (100 micro;M) Formulation PBS buffer Storage...
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075: Why Biotin Can Mess Up Your Labs (Big Time)
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BioFlax 20 is ...the hands-down choice for coat, skin and hoof problems - Horse Journal, June 2000 issue. Biotin supplementation has been shown to be helpful in improving hoof qu...
BTD and Children - weight gain
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Biotinylation Reagents
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