Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...
Ink-jet printing has been demonstrated as a manufacturing technique that facilitates the rapid, reproducible and economical production of amperometric glucose biosensors. Glucose was chosen as the analyte for demonstrating the process. For eight electrodes produced, the relative standard deviation of the response was less than 5%. The technique is extremely versatile, and will enable a wide variety of reagents to be placed on virtually any sensor design. This technique will be of particular benefit for the mass manufacture of intricate devices, where existing production techniques, such as screen-printing, may not be suitable.. ...
A biosensor system determines analyte concentration from an output signal generated by an oxidation/reduction reaction of the analyte. The biosensor system adjusts a correlation for determining analyte concentrations from output signals at one temperature to determining analyte concentrations from output signals at other temperatures. The temperature-adjusted correlation between analyte concentrations and output signals at a reference temperature may be used to determine analyte concentrations from output signals at a sample temperature.
Lead Researcher: Dr Katrina Campbell. For more than a decade the Advanced Asset laboratory has been pioneering the use of biosensor technology in the area of food safety monitoring and we are recognised as world-leading research group in this area. We are experts in the development of highly innovative sensor based detection systems relating to biological and chemical contaminants in foods of pertinence to food integrity, safety, defence, quality and sustainability. Examples of such contamination include: naturally occurring toxins of fungal, algal, plant and bacterial origin present in the environment (e.g. mycotoxins and marine biotoxins); improper use of agrichemicals (e.g. antibiotics, pesticides), the illegal use of growth promoting compounds in animal production (e.g. B-agonists), by-products from food processing (e.g. acrylamide, allergens) and food-borne pathogens.. In addition to the traditional enzyme and luminescence based immunoassay design capabilities (e.g. ELISA and LFDs) the ...
A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.
Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed
In recent years, there has been rapidly growing interest in developing hand held, sensitive and cost-effective on-chip biosensing systems that directly translate the presence of certain bioanalytes (e.g., biomolecules, cells and viruses) into an electronic signal. The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. All necessary sample handling and analysis steps are then performed within the chip. Microfluidic systems for biomedical analysis usually consist of a set of units, which guarantees the manipulation, detection and recognition of bioanalytes in a reliable and flexible manner. Additionally, the use of magnetic fields for performing the aforementioned tasks has been steadily gaining interest. This is because magnetic fields can be well tuned and applied either externally or from a directly integrated solution in the biosensing system. In combination with these applied magnetic
Ms. Witz led GE Healthcares Healthcare Systems Interventional global business, which researches and develops innovative medical technologies to help physicians diagnose and treat disease earlier, faster and more accurately. Leading the GE Medical Diagnostics team is an honor, said Witz. Im eager to continue the mission of advancing medicine through novel diagnostic agents. We will raise the bar in diagnosing critical diseases such as Parkinsons disease, Alzheimers and heart failure.. Pascale has a breadth of experience that makes her an ideal person to lead the Medical Diagnostics business, said Dineen. She brings an extensive background in molecular biology as well as imaging, both of which are critical to driving Medical Diagnostics development of its portfolio of innovative molecular diagnostic imaging agents.. For more information: www.gehealthcare.com. ...
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Biosensors are devices that use biological components to detect important analytes. Biosensing systems have various applications in areas such as medicine, environmental monitoring, and process control. Classical biosensors are often based on bacteria or purified enzymes that have limitations on efficiency or stability. I have developed several new biosensors to overcome these disadvantages. Two preliminary biosensors were first created based on the extremely strong and specific interaction between biotin and (strept)avidin. Both biosensors showed high sensitivity and reliability for measuring biotin with detection limits of 50-1000 pg/ml and 20-100 ng/ml, respectively. Following these, a new biosensor was developed by coupling a mobile, functionalized microsurface with cell-free expression approaches. This biosensor demonstrated a dynamic range of 1- 100 ng/ml. In addition, I also explored the possibility of combining these biosensing systems with engineered living cells. By leveraging the ...
BioTek Notas de Aplicações, 08-Nov-13, Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
To boost our understanding of Taspase1s substrate specificity we used our biosensor assay mixed with positional scanning mutagenesis
Biosensors are poised to make a large impact in environmental, food, and biomedical applications, as they clearly offer advantages over standard analytical methods, including minimal sample preparation and handling, real-time detection, rapid detection of analytes, and the ability to be used by non-skilled personnel. Covering numerous applications of biosensors used in food and the environment, Portable Biosensing of Food Toxicants and Environmental Pollutants presents basic knowledge on biosensor technology at a postgraduate level and explores the latest advances in chemical sensor technology for researchers. By providing useful, state-of-the-art information on recent developments in biosensing devices, the book offers both newcomers and experts a roadmap to this technology. In the book, distinguished researchers from around the world show how portable and handheld nanosensors, such as dynamic DNA and protein arrays, enable rapid and accurate detection of environmental pollutants and pathogens. ...
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
A novel biosensing approach for the label-free detection of nucleic acid sequences of short and large lengths has been implemented, with special emphasis on targeting RNA sequences with secondary structures. The approach is based on selecting 8-aminoadenine-modified parallel-stranded DNA tail-clamps as affinity bioreceptors. These receptors have the ability of creating a stable triplex-stranded helix at neutral pH upon hybridization with the nucleic acid target. A surface plasmon resonance biosensor has been used for the detection. With this strategy, we have detected short DNA sequences (32-mer) and purified RNA (103-mer) at the femtomol level in a few minutes in an easy and level-free way. This approach is particularly suitable for the detection of RNA molecules with predicted secondary structures, reaching a limit of detection of 50fmol without any label or amplification steps. Our methodology has shown a marked enhancement for the detection (18 for short DNA and 54 for RNA), when compared ...
Books Biofunctionalization of Nanomaterials Nanotechnologies for the Life Sciences, Vol. 1 Edited by Challa Kumar. Wiley-VCH, Weinheim 2005. 425 pp., hardcover E 139.00.-ISBN 3-527-31381-8 With this first volume, Biofunctionalization of Nanomaterials, Wiley-VCH and the editor, Challa Kumar, introduce a series that has an ambitious aim. The plan is for a ten-volume encyclopedia that will collect together and critically evaluate all aspects of the current state of knowledge about the use of nanotechnology in the life sciences. This is certainly one of the most dynamic areas of research at present, and therefore to attempt a comprehensive summary and evaluation is a challenging task. Moreover, this field has the distinction that, perhaps more than any other current area of research, it has inputs from chemistry, biology, biochemistry, materials science, and physics. Therefore, it is important that the knowledge must be communicated in a way that extends beyond the boundaries of the classical ...
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A new approach is presented to obtain fluorescent sensors for pH windows that work in water and under biomimetic conditions. A single molecule that features all-covalently linked components is used, thus making it capable of working as a fluorescent sensor with an OFF/ON/OFF response to pH value. The components are a tertiary amine, a pyridine, and a fluorophore (pyrene). The forms with both protonated bases or both neutral bases quench the pyrene fluorescence, whereas the form with the neutral pyridine and protonated amine groups is fluorescent. The molecular sensor is also equipped with a long alkyl chain to make it highly hydrophobic in all its protonated and unprotonated forms, that is, either when neutral or charged. Accordingly, it can be confined at any pH value either in traditional (i.e., low-molecular-weight) nonionic surfactant micelles or inside polymeric, biocompatible micellar containers. Relevant for future applications in vivo, thanks to its strong hydrophobicity, no leakage of ...
The aim of this work is the development of a cost effective, rapid, portable analysis system for the measurement of bioavailable toxic metal or organic contaminants, which may be present at natural attenuation landfill sites requiring remediation. The proposed system is intended to be used as a tool in the monitoring or characterisation of a site by discrete measurement. A portable system will be developed, comprising a miniaturised extraction and separation device employing capillary electrophoresis, detectors for specific analyses (metals or organics) by biosensors or immunosensors and dedicated instrumentation to process the signals generated. Development of extraction procedures for the analysis of plant material will also be part of the project, to be used when phytoremediation is employed as part of the site remediation process. Biosensors to detect toxic metal will use bioengineered proteins to interact with bioavailable metals on an electrode surface. The conformational change in the
TY - JOUR. T1 - Fluorescent alizarin-phenylboronic acid ensembles. T2 - Design of self-organized molecular sensors for metal ions and anions. AU - Kubo, Yuji. AU - Ishida, Tomohisa. AU - Kobayashi, Atushi. AU - James, Tony D.. PY - 2005/7/21. Y1 - 2005/7/21. N2 - Alizarin-phenylboronic acid interactions were used in a new design strategy for self-organized fluorescent sensors of metal ions and anions. First, Alizarin Red S (ARS), which binds effectively to phenylboronic acid (PBA) at neutral conditions in water, proved to be an excellent fluorescent indicator for the selective detection of metal ions; the fluorescent-active ARS-PBA ensemble causes an efficient and selective quenching in the fluorescence by adding metal ions. It was also found that anion-induced phenylboronates can bind effectively to alizarin and greatly enhance the fluorescence in MeOH. The observation makes it possible to design new self-organized anion sensory systems composed of alizarin (as reporter) and phenylboronic acids ...
We describe a novel biosensor system for reporting proximity between cell surface proteins in live cultured cells. The biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules. To demonstrate feasibility for the approach, two recombinant rapamycin-binding proteins were expressed as...
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Neural Networks for an Analysis of the Hemometabolites Biosensor Response: 10.4018/ijehmc.2013100106: In this work, the concentration dependent response of amperometric biosensor array for the biomarkers glucose, cholesterol and urease was explored, using
TY - JOUR. T1 - Engineered cells as biosensing systems in biomedical analysis. AU - Raut, Nilesh. AU - OConnor, Gregory. AU - Pasini, Patrizia. AU - Daunert, Sylvia. N1 - Funding Information: Acknowledgments This work was supported in part by the National Science Foundation (grant CHE-0416553); the National Institute of Environmental Health Sciences, Superfund Research Program (grant P42ES07380); the Broad Foundation, Broad Medical Research Program (grant IBD-0198R); the National Institute of Hometown Security; and the Childrens Miracle Network. S.D. is grateful for support from the Lucille P. Markey Chair in Biochemistry and Molecular Biology of the Miller School of Medicine of the University of Miami, as well as from a Gill Eminent Professorship from the University of Kentucky. N. R. acknowledges support from a Research Challenge Trust Fund Fellowship from the University of Kentucky.. PY - 2012/4. Y1 - 2012/4. N2 - Over the past two decades there have been great advances in biotechnology, ...
Contamination of drinking water with arsenic is a recurring problem in both industrialized and developing countries. Supplies for large populations can have concentrations much higher than the permissible levels, set by the World Health Organization (WHO) to 10 μg As/L for most European countries and the United States and to 50 μg As/L elsewhere. As arsenic analysis requires high-end instruments, which are largely unavailable in developing countries, bioassays based on genetically engineered bacteria have been proposed as suitable alternatives. Yet, such tests would profit from better standardization and direct incorporation into sensing devices. The final objective of this work was to develop a microfluidic device in which bacterial bioreporters could be embedded, actively maintained for at least one week, exposed to arsenic and which allowed direct detection of the reporter signal produced, as a further step towards a complete miniaturized bacterial biosensor. The signal element in the biosensor is
The importance of nanotechnology in medical technologies, especially biomedical diagnostics, is indubitable. By taking advantages of nanomaterials, many medical diagnostics methods have been developed so far, including electrochemical nanobiosensors. They have been used for quantification of different clinical biomarkers for detecting, screening, or follow up a disease. microRNAs (miRNAs) are one of the most recent and reliable biomarkers used for biomedical diagnosis of various diseases including different cancer types. In addition, there are many electrochemical nanobiosensors explained in publications, patents, and/or a commercial device which have been fabricated for detection or quantification of valuable miRNAs. The aim of this article is to review the concept of medical diagnostics, biosensors, electrochemical biosensors and to emphasize the role of nanotechnology in nanobiosensor development and performance for application in microRNAs detection for biomedical diagnosis. We have also summarized
The global Glucose Biosensor Market is anticipated to witness lucrative growth during the period of 2015 to 2022. The homecare diagnostics Glucose Biosensor Market is projected to flourish over the forecast period. Ease-of-use, quick results, and low costs of the tests are some aspects supporting market growth. On the other hand, the hospital-based Glucose Biosensor Market is projected to grow at a significant CAGR of 10.3% during the period 2015 to 2022.. Browse Detail Report With TOC @ http://www.hexareports.com/report/glucose-biosensor-market/details. Some key factors attributing to the growth of this market include the increasing incidence of diabetes across the globe, development of technologically advanced products, growing demand for minimally and non-invasive products, patient awareness levels, and the need for early diabetes detection. However, presence of other alternatives for management of the diabetes, such as laboratory methods is expected to restrict market growth.. Request A ...
Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...
Genetically-encoded fluorescent sensor proteins are attractive tools for studying intracellular Zn2+ homeostasis and signaling. Here we provide an overview of recently developed sensors based on Förster Resonance Energy Transfer (FRET). The pros and cons of the various sensors are discussed with respect to Z Iron and zinc sensing in cells and the body
The Nova StatStrip Glucose Hospital Meter System (StatStrip) and the StatStrip Xpress2 Glucose Hospital Meter System (StatStrip Xpress2) are the only glucose analyzers cleared by the FDA for use in all types of hospital patients, including critically ill patients receiving intensive medical intervention. For healthcare settings other than critical care, StatStrip and StatStrip Xpress2 are cleared for use with capillary finger stick, arterial, venous, neonatal heel stick and neonatal arterial specimens. When using the system to measure glucose on critically ill patients, StatStrip and StatStrip Xpress2 are cleared for use with arterial, venous, neonatal heel stick and neonatal arterial specimens. StatStrip and StatStrip Xpress2 are not cleared for capillary specimens on patients receiving intensive medical intervention/therapy because capillary samples from these patients, particularly those with decreased peripheral blood flow, may not truly reflect a patients physiological state. Some ...
Researchers at Harvard University have developed a non-toxic wearable sensor consisting of a silicon-rubber that attaches to the hand and capable of mearin | Technology
Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.. One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...
The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...
Boyce Thompson Insitute scientist Maria Harrison will collaborate with Wayne Versaw of Texas A&M University to create a new imaging system to visualize and measure the movement of phosphate from soil fungi into plant root cells in a $1.2 million DOE-funded project.
TY - JOUR. T1 - A contour-mode film bulk acoustic resonator of high quality factor in a liquid environment for biosensing applications. AU - Xu, Wencheng. AU - Choi, Seokheun. AU - Chae, Junseok. PY - 2010/2/17. Y1 - 2010/2/17. N2 - This letter reports an acoustic resonator of high quality factors (Qs) operating in liquid media. The film bulk acoustic resonator (FBAR) is made of a ring-shaped piezoelectric aluminum nitride thin film, and is excited in a contour mode. By having a low motional resistance upon coupling with liquids, the contour mode FBAR achieved Qs up to 189, more than 12× over the state-of-the-art FBARs in liquids. The resonator was characterized by an aptamer-thrombin binding pair for a biosensor and showed a mass resolution of 1.78 ng/ cm2.. AB - This letter reports an acoustic resonator of high quality factors (Qs) operating in liquid media. The film bulk acoustic resonator (FBAR) is made of a ring-shaped piezoelectric aluminum nitride thin film, and is excited in a contour ...
This thesis treats the development of an integrated optical sensor array. The sensors are slot-waveguide ring resonators, integrated with on-chip surface grating couplers and light splitters, for alignment tolerant, real-time, refractive index sensing, and label-free biosensing. The work includes: the design of components and system layouts, the development of fabrication methods, the fabrication of sensor chips, the characterization of the chips, and the development of physical system models for accurate extraction of resonance wavelengths in measured spectra.. The main scientific achievements include: The evaluation of a novel type of nano-structured optical waveguide for biochemical sensing. The realization of an array of such slot-waveguide sensors, integrated with microfluidic sample handling, for multiplex assays. The first study of the thermal behavior of slot-waveguide sensors and the discovery of unique temperature compensation capabilities.. From an application perspective, the use of ...
View details for this PhD Studentship: The Computational Design of Drug Sensors and Adsorbents for Environmental Applications job vacancy at...
TY - JOUR. T1 - The ongoing quest for understanding the novel environmental applications and implications of nanotechnology. AU - Cledon, Maximiliano. AU - Hristovski, Kiril. PY - 2018/3/15. Y1 - 2018/3/15. UR - http://www.scopus.com/inward/record.url?scp=85040322403&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85040322403&partnerID=8YFLogxK. U2 - 10.1016/j.scitotenv.2017.09.122. DO - 10.1016/j.scitotenv.2017.09.122. M3 - Editorial. C2 - 29066206. AN - SCOPUS:85040322403. VL - 618. JO - Science of the Total Environment. JF - Science of the Total Environment. SN - 0048-9697. ER - ...
Environmental Applications of Nanoscale and Microscale Reactive Metal Particles takes the reader from current laboratory studies in the function and use of these particles through scale-up and finally to the state-of-the-science in field-scale demonstrations and applications.
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
Purchase Supercritical Fluid Technology for Energy and Environmental Applications - 1st Edition. Print Book & E-Book. ISBN 9780444626967, 9780444626974
A molecular sensor or chemosensor is a molecule that interacts with an analyte to produce a detectable change. Molecular sensors combine molecular recognition with some form of reporter so the presence of the guest can be observed. The term supramolecular analytical chemistry has recently been coined to describe the application of molecular sensors to analytical chemistry. Early examples of molecular sensors are crown ethers with large affinity for sodium ions but not for potassium and forms of metal detection by so-called complexones which are traditional pH indicators retrofitted with molecular groups sensitive to metals. This receptor-spacer-reporter concept is a recurring theme often with the reporter displaying photoinduced electron transfer (PET). One example is a sensor sensitive to heparin. Other receptors are sensitive not to a specific molecule but to a molecular compound class. One example is the grouped analysis of several tannic acids that accumulate in ageing Scotch whisky in oak ...
The simultaneous detection of multiple analytes is an important consideration for the advancement of biosensor technology. Currently, few sensor systems possess the capability to accurately and precisely detect multiple antigens. This work presents a simple approach for the functionalization of sensor surfaces suitable for multichannel detection. This approach utilizes self-assembled monolayer (SAM) chemistry to create a nonfouling, functional sensor platform based on biotinylated single-stranded DNA immobilized via a streptavidin bridge to a mixed SAM of biotinylated alkanethiol and oligo(ethylene glycol). Nonspecific binding is minimized with the nonfouling background of the sensor surface. A usable protein chip is generated by applying protein-DNA conjugates which are directed to specific sites on the sensor chip surface by utilizing the specificity of DNA hybridization. The described platform is demonstrated in a custom-built surface plasmon resonance biosensor. The detection capabilities of ...
Worden, a professor in the MSU Department of Chemical Engineering and Materials Science (CHEMS), began working in oxidation-reduction reactions, also known as redox, in the late 1990s. These chemical reactions are important in a number of areas, including biofuel production. As the research progressed, Worden developed expertise in nanotechnology and biocatalysts, which are often used to perform chemical transformations on organic compounds.. Over the years, various stages of this project received funding from the National Science Foundation (NSF) and other organizations. In looking at various aspects of these reactions and trying to exploit their economic benefits, Worden developed a biosensor system that was recently patented by MSU.. Now Worden is collaborating on a new NSF project with Paul Satoh, MSU adjunct professor in engineering and food science and former vice president of research at Neogen, a Lansing-based company that develops and markets products dedicated to food and animal ...
Global Gold Nanoparticles Market is expected to reach USD 4.86 billion by 2020, according to a new study by Grand View Research, Inc. Medical & dentistry was the largest end-use segment for the gold nanoparticles and accounted for over 50% of total demand in 2013. Growing metal nanomaterials use in medical diagnostics & imaging especially for drug delivery systems in cancer & tumor cell detection is expected to drive gold nanoparticles demand over the forecast period.. North America dominated the global gold nanoparticles market accounting for over 30% of global volume in 2013. Increasing R&D spending by individual nanotechnology companies and universities along with expanding medical diagnostics industry is expected to be the major factors driving gold nanoparticles demand in the region. Asia Pacific is estimated to witness the fastest growth at a CAGR of over 25% from 2014 to 2020. Growth of nanomaterials industry in countries including China, Taiwan, South Korea and Japan along with emergence ...
ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHORS REQUEST.] Low-temperature plasma polymerization was performed to coat the individual nanomaterial not only for better suspension but also for thermal conductivity enhancement with water. With the increased hydrophilicity of nanomaterials surfaces, more stable nanomaterials-in-water suspension resulted in the anomalous thermal conductivity increase of fluid. Electrochemical DNA biosensor is the device to detect the DNA specifically related to the pathogenic microbes. A basic DNA biosensor is designed by the immobilization of a single-stranded oligonucleotide (probe) on a transducer surface to recognize its complementary (target) DNA sequence via hybridization. Cyclic voltammetry (CV) and direct pulse voltammetry (DPV) were used to measure the electrochemical response before and after target hybridization with two red-ox indicators, Ru(III) and Fe(III). Consequently, developed nanostructured microelectrode exhibited atto-molar sensitivity ...
en] Surface plasmon resonance (SPR)-based biosensors are very powerful tools for the study of biomolecular interactions, chemical detection and immunoassays. This paper reviews the performance of various SPR structures and detection schemes focusing on propagating surface plasmons generated in planar structures. Some aspects of their surface functionalization, the key element which imparts biofunctionality to these structures and hence transforming them into biosensors, will also be discussed accordingly. The ultimate performance of SPR-based biosensors will thus be determined by both their inherent optical performance and suitable surface functionalization. (C) 2011 Elsevier Ltd. All rights reserved ...
The scope of this thesis is the synthesis of nanostructured materials, their functionalization and use for optical lactate biosensing applications. Rapid detection of L-lactate is important in many applications in the clinical sector, in the food industry, or in biotechnology. The formation of enzyme loaded nanostructured materials is a promising approach to obtain performing, reliable and stable enzyme-based optical biosensors. Two different sensing schemes are proposed: the development of lactate-responsive films (pathches) and the development of a microparticle based lactate detection system. The content of this work can be divided in three main tasks: (i) the synthesis of nanostructured support materials for enzyme immobilization, (ii) the functionalization of these materials towards lactate detection, and (iii) the assessment of the performance and sensitivity of these enzyme-loaded films and particles for biosensor applications. The design of porous supports aims at providing large surface ...
Handheld electrochemical sensors are part of the daily routine for millions of people with diabetes around the globe who monitor their blood sugar levels with electric glucometers. While such sensors have revolutionized at-home medical testing for diabetics, they have not yet been successfully applied to diagnosing other conditions. Sensors like glucometers detect glucose in blood based on the activity of an enzyme, and there are only a limited number of enzymes that can be used to sense biomarkers of human disease. An alternative detection strategy based on binding events between antibodies and their molecular targets have been investigated to expand the use of electrochemical sensors for medicine, but these sensors fall victim to the rapid accumulation of fouling substances from biological fluids on their conductive surfaces, which deactivate them. Existing antifouling coatings are difficult to mass-manufacture, suffer from quality and consistency issues, and are not very effective.. ...
Over the past decade, the development and applications of immunoassays have continued to grow exponentially. This book focuses on some of the latest advances in immunoassay technology, which include new materials and methods. The book contains nine invited chapters that are divided into three sections. In the first section, the basics for producing recombinant antibodies, the use of polyacrylonitrile fibre as a solid surface, and the nature of interference in immunoassays are summarized. The second section begins with a chapter on the basic concepts of different types of immunosensors, some of which allow label-free detection of specific analytes. This is followed by chapters on piezoelectric immunosensors and surface plasmon resonance biosensors. A chapter on using nanopores as a label-free biosensing platform and its potential for immunosensing is also included in the second section. The third section starts with a chapter that describes different platforms for carrying out multiplexed ...
TY - JOUR. T1 - Oligonucleotide-based systems. T2 - DNA, microRNAs, DNA/RNA aptamers. AU - Jolly, Pawan. AU - Estrela, Pedro. AU - Ladomery, Michael. N1 - Special volume Biosensor technologies for detection of biomolecules (Ed: P. Estrela) PY - 2016/6/30. Y1 - 2016/6/30. N2 - There is an increasing number of applications that have been developed for oligonucleotide-based biosensing systems in genetics and biomedicine. Oligonucleotide-based biosensors are those where the probe to capture the analyte is a strand of DNA, RNA or a synthetic analogue to naturally occurring nucleic acids. This chapter will draw light upon various types of nucleic acids such as DNA, RNA (particularly microRNAs), their role and their application in biosensing. Also, it will cover DNA/RNA aptamers, which can be used as bioreceptors to a wide range of targets such as proteins, small molecules, bacteria and even cells. It will also highlight how the invention of synthetic oligonucleotides like PNA or LNA has pushed the ...
Ultrafast molecular sensors, used with high-speed multiphoton microscopy, enable direct visualization of chemical changes in the brain. Rumiana Bakalo
This dissertation presents one-dimensional nanostructures for novel biosensors and transparent electronics applications. In chapter 1, background information regarding nanomaterials studied in this dissertation is described. ❧ In chapter 2, I describe the first application of antibody mimic proteins (AMPs) in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at sub-nanomolar concentration in the presence of 44 µM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. ❧ In chapter 3, I demonstrate an In2O3 nanowire-based biosensing system that is capable of performing rapid, label-free, electrical detection of cancer biomarkers directly from human whole blood collected by a ...
This dissertation presents one-dimensional nanostructures for novel biosensors and transparent electronics applications. In chapter 1, background information regarding nanomaterials studied in this dissertation is described. ❧ In chapter 2, I describe the first application of antibody mimic proteins (AMPs) in the field of nanobiosensors. In2O3 nanowire based biosensors have been configured with an AMP (Fibronectin, Fn) to detect nucleocapsid (N) protein, a biomarker for severe acute respiratory syndrome (SARS). Using these devices, N protein was detected at sub-nanomolar concentration in the presence of 44 µM bovine serum albumin as a background. Furthermore, the binding constant of the AMP to Fn was determined from the concentration dependence of the response of our biosensors. ❧ In chapter 3, I demonstrate an In2O3 nanowire-based biosensing system that is capable of performing rapid, label-free, electrical detection of cancer biomarkers directly from human whole blood collected by a ...
By Ajit Sadana. Biosensors are discovering expanding purposes in several components. during the last few years the parts the place biosensors can be utilized successfully has elevated dramatically. This publication just like the past 4 books on analyte-receptor binding and dissociation kinetics via this writer addresses the customarily missed sector. The kinetics of binding and dissociation in technique to acceptable receptors immobilized on biosensor surfaces happens less than diffusional barriers on established surfaces. The receptors immobilized at the biosensor floor give a contribution to the measure of heterogeneity at the sensor chip floor. The fractal research examples provided through the publication offer a handy capability to make quantitative the measure of heterogeneity current at the sensor floor, and relates it to the binding and dissociation cost coefficients. The fractal measurement is a quantitative degree of the measure of heterogeneity current at the biosensor floor. The ...
1. A system comprising: a first medical device including: a sensor signal processor configured to receive a plurality of electrical sensor signals produced by a plurality of sensors and at least one sensor signal produced by an implantable sensor; a decision circuit configured to apply a detection threshold to a sensor signal to determine whether an event associated with worsening heart failure (HF) occurred in a subject, and to output an indication of whether the event associated with worsening HF occurred to a user or process; and a first communication circuit configured to communicate information with a second separate device; and a second medical device including: a second communication circuit configured to communicate information with the first device; a port configured to receive information from a third device via a communication network, wherein the information indicates a co-morbidity of a subject; a sensor signal selection circuit configured to select a sensor signal to monitor from ...
The Journal of Nanotechnology is a peer-reviewed, Open Access journal that reports original research, as well as review articles, in all areas related to the science and technology of nanosized and nanostructured materials. Importantly, a special emphasis in published work is placed on materials design, characterisation, functionality, and preparation for implementation in systems and devices. The journal is naturally multidisciplinary, and welcomes submissions across biological, physical, engineering and computer sciences. Contributions from both academia and industry are equally encouraged.
Solar visible light is a source of clean and cheap energy. Herein, a new kind of hierarchical CuO/ZnO nanomaterial was synthesized using a facile process. Characterized by FESEM, TEM, XRD, XPS, and so forth, this CuO/ZnO naomaterial shows a special hierarchical nanostructure with CuO nanoparticles grown on ZnO nanorods. By assembling the hierarchical CuO/ZnO nanomaterials on a piece of commercial glassfiber membrane, a novel hierarchical CuO/ZnO membrane was fabricated. This CuO/ZnO membrane demonstrated excellent environmental applications, such as improved photodegradation of contaminants and antibacterial activity, under the irradiation of visible light. Compared with pure ZnO nanorod membrane, the improved photodegradation and antibacterial capacities of this hierarchical CuO/ZnO membrane result from the special hierarchical nanostructure of CuO/ZnO nanomaterials, which could enhance light utilization rate, enlarge specific surface area, and retard the recombination of electrons and holes at ...
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Reduced Graphene Oxide (rGO) is a good absorber of light, and is a potential material for use in photothermal therapy. rGO-based nanocomposites have been considered for cancer theranostics, but this method has rarely been used for pathogen destruction. This is even more surprising considering the widespread commercial availability of graphene oxide.. Graphenea explored the possibility of destroying E. coli pathogens in its study featured in the Journal of Materials Chemistry B. Reduced graphene oxide (rGO-PEG- NH2) and Au nanorods (Nrs) coated with rGO-PEG-NH2 were used to destroy the pathogens by laser irradiation. The rGO-PEG coating serves a dual purpose by mitigating the toxicity of Au NRs and improving the overall photo-thermal process and so the temperatures reachable.. In this paper, Graphenea showed 99% bacterial killing efficiency at low concentrations (20-49mg/ml) in a water solution, demonstrating the potential of reduced graphene oxide as an effective anti-pathogen agent.. In another ...
Get this from a library! The physical chemistry of materials : energy and environmental applications. [Rolando M A Roque-Malherbe] -- This text provides comprehensive data on the physical chemistry of materials used in these resources, including adsorbents, ion exchangers and conductors, catalysts, and permeable materials.
Aptamers, which are in vitro-selected functional oligonucleotides, have been employed to design novel aptasensor due to their inherent high selectivity and affinity compared to traditional biorecognition elements. This report presents a novel aptamer biosensor for determining the endocrine disrupting compound (EDC), 17~-estradiol (E2), which was constructed from a SELEX-synthesized 76-mer biotinylated aptamer for 17~-estradiol incorporated in a dendritic generation 1 poly(propylene imine)- poly thiophene (G 1PPT-co-PEDOT) star copolymer-functionalised Au electrode via biotin-avidin interaction. The sensor platform and aptasensor were interrogated with scanning electron microscopy (SEM), FTIR, electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and square wave voltammetry (SWV). The kinetic parameters of the sensor platform were determined by modelling the [Fe(CN)6r3/ - 4 (redox probe) Nyquist and Bode impedimetric spectra to the appropriate equivalent electrical circuit. The ...
PROMEGA NOTES WWW.PROMEGA.COM NUMBER 100 SEPTEMBER 2008 LIVE-CELL BIOSENSOR is required. A standard luminometer with injectors is suf- Intracellular biosensor technologies remain an area of keen ficient to detect signal readout and obtain real-time interest that until recently was dominated mostly by flu- kinetic data, all without any loss in the information that orescent approaches. The most common examples are can be collected. As evident in Figure 2, signal kinetics FRET-based biosensors using variants of GFP. However, of reversible agonists and antagonists can be obtained in FRET-based biosensors suffer from low dynamic range of minutes following treatment. Important to HTS applica- response due to characteristics inherent in the detection tions, these assays can be performed at room temperature modality (2). The results are FRET ratios of 30-100% or with little difference in the range of response and more 2-fold response at best, which can be a significant draw- stable kinetics ...
Human papillomavirus (HPV) is one of the most common sexually transmitted disease, transmitted through intimate skin contact or mucosal membrane. The HPV virus consists of a double-stranded circular DNA and the role of HPV virus in cervical cancer has been studied extensively. Thus it is critical to develop rapid identification method for early detection of the virus. A portable biosensing device could give rapid and reliable results for the identification and quantitative determination of the virus. The fabrication of electrochemical biosensors is one of the current techniques utilized to achieve this aim. In such electrochemical biosensors, a single-strand DNA is immobilized onto an electrically conducting surface and the changes in electrical parameters due to the hybridization on the electrode surface are measured. This review covers the recent developments in electrochemical DNA biosensors for the detection of HPV virus. Due to the several advantages of electrochemical DNA biosensors, their ...
The focus of the research reported in this dissertation has focused on two areas: (1) Synthesis of silane-containing polymers and (2) the application of these polymers to surface modification of substrates such as PDMS (for microfluidic applications), glass, quartz, silicon wafers and various nanoparticles (magnetic iron oxide, silica) for use in chemical and biological sensing and separation applications as well as in biofunctionalization of magnetic nanoparticles. Chapter 1 of this dissertation described the preparation of a library of silane containing co- and terpolymer prepared with various substituted maleimides along with their characterization by FTIR, UV-Visible, 1H- and 13C-NMR, TGA and DSC. The ability to control the reactive functionalities incorporated into the polymer structure affords direct control over the resulting properties of the materials. Chapter 2 describes the conversation of the polymers prepared in chapter 1 into various functional forms that can be used in the design of
3D printing technologies are currently appealing for the research community due to their demonstrated versatility for different scientific applications. One of the most commonly used materials for 3D printing is polylactic acid (PLA), a biodegradable polymer that can be fully or partially digested by enzymes Nanocarbons
This paper presents a novel ultrasensitive and rapid impedimetric biosensor with new immobilisation materials for parathyroid hormone (PTH) with the aim to determine the PTH level in serum for the diagnosis and monitoring of parathyroid diseases such as hyperparathyroidism, adenoma, and thyroid cancer. The interaction between PTH and the biosensor was investigated with an electrochemical method. The biosensor was based on the gold electrode modified by mercaptohexanol (6-MHL). Anti-parathyroid hormone (anti-PTH) was covalently immobilised onto a self-assembled monolayer (SAM) by using epiclorhidrina (EPI) with ethanolamine (EA). The EPI-EA interaction represents the first use of these for the construction of biosensors in published reports. The immobilisation of the anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM) techniques. After the optimisation studies of immobilisation materials such as 6-MHL, EPI, EA and ...
The Journal of The Electrochemical Society is publishing a focus issue dedicated to the late Professor Nongjian Tao for his longstanding contribution to the development of chem/biosensors and nanoscale measurements. This issue also serves as a celebration of the 70th birthday of Professor Stuart Lindsay, Professor Taos doctoral advisor and longtime collaborator for over 35 years.. Contributions are invited in all aspects of chem/biosensor technology with topics that include, but are not limited to: Electric, magnetic, and optical biosensors; novel transduction modes; molecular recognition elements; nanomaterials and their system level integration for biological/medical applications such as point-of-care, implantable, and wearable sensors. In addition, novel nanoscale measurements of cells and cellular/molecular components (e.g., nucleic acid, proteins, organic molecules), including scanning probe microscopy, plasmonic, and optical imaging of biomolecules, are also highly encouraged. Many of ...
A technology for detecting chemical vapors using low power, low cost, tunable microsensors. This technology is based on metal nanoparticles encapsulated by a single layer of organic molecules.
A composition of redox-reagent containing metal-containing complex and thionine or its derivative as electron transfer mediator for use in an electrochemical biosensor, and a biosensor containing the same are provided. With the increase in reaction rate between redox enzyme-thionine (or its derivati
Biosensor technology is a powerful alternative to conventional analytical techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. One of the factors that affect the performance of a biosensor is the immobilization of the biological sensing element, or bioreceptor, on the transducer surface. The objectives of this study were to determine the effects of polymer concentration, ultraviolet (UV) light exposure, and film thickness on the activity and stability of horseradish peroxidase (HRP) and alcohol oxidase (AOX) when immobilized in a photo-crosslinkable and water-soluble polymer, poly (vinyl alcohol) azide-unit water pendant (PVA-AWP). The immobilized enzyme films were to be used in biosensing applications and their stability and activity were determined colorimetrically and electrochemically. Compared to other gel immobilization techniques, PVA hydrogels offer several advantages, such as better elasticity, low-toxicity, biocompatibility with ...
Surface plasmon resonance (SPR) biosensors are used to characterize and quantify biomolecular interactions. Real-time and fast measurement, high sensitivity and specificity, no need of labeled reagents are the unique properties of SPR biosensors. SPR biosensors have been used for detection of several analytes. Recently, molecular imprinting technology is used for creation of biorecognition surfaces on the SPR biosensors. Molecular imprinting is the technology of designing materials with robust recognition sites suitable for interaction with the print molecule. Due to their stability in complex matrices under tough conditions, ease of preparation, low cost, reusability, high recognition capacity to the template molecule and binding characteristics as high as those of natural binders, molecularly imprinted polymers (MIPs) are extensively used in a variety of areas, such as catalysis, separations, solid-phase extractions and biosensors. In this study, an SPR biosensor was prepared for prostate ...
BACKGROUND: Direct acting antivirals (DAAs) provide efficient hepatitis C virus (HCV) therapy and clearance for a majority of patients, but are not available or effective for all patients. They risk developing HCV-induced hepatocellular carcinoma (HCC), for which the mechanism remains obscure and therapy is missing. Annexin A2 (AnxA2) has been reported to co-precipitate with the non-structural (NS) HCV proteins NS5B and NS3/NS4A, indicating a role in HCC tumorigenesis and effect on DAA therapy.. METHODS: Surface plasmon resonance biosensor technology was used to characterize direct interactions between AnxA2 and HCV NS5B, NS3/NS4 and RNA, and the subsequent effects on catalysis and inhibition.. RESULTS: No direct interaction between AnxA2 and NS3/NS4A was detected, while AnxA2 formed a slowly dissociating, high affinity (K D = 30 nM), complex with NS5B, decreasing its catalytic activity and affinity for the allosteric inhibitor filibuvir. The RNA binding of the two proteins was independent and ...
A. Heiniger, B. L. Miller, and P. M. Fauchet, Limits of Diffusive Transport to an Optical Biosensor and the Impact of Optical Forces, in Frontiers in Optics 2012/Laser Science XXVIII, OSA Technical Digest (online) (Optical Society of America, 2012), paper FTu4C.7 ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Bioelectron. 19, 1497-1504. Oh, B. , Chun, B. , Bae, Y. , Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation of Escherichia coli O157:H7 using O157 specific antibody coated beads. Lee, W. , and Choi, J. W. (2005). The fabrication of protein chip based on surface plasmon resonance for detection of pathogens. Biosens. Bioelectron. 20, 1847-1850. , and Matsunaga, T. (1999). Electrochemical detection of allergen in small-volume whole blood using an array microelectrode: A simple method for detection of allergic reaction. Biotechnol. Bioeng. 65, 480-484. Okrend, A. J. , Rose, B. , and Lattuda, C. P. (1992). Isolation ...
Cardiac myocytes have provided a key paradigm for the concept of the compartmentalized cAMP generation sensed by AKAP-anchored PKA. Phosphodiesterases (PDEs) provide the sole route for degrading cAMP in cells and are thus poised to regulate intracellular cAMP gradients. PDE3 and PDE4 represent the major cAMP degrading activities in rat ventriculocytes. By performing real-time imaging of cAMP in situ, we establish the hierarchy of these PDEs in controlling cAMP levels in basal conditions and on stimulation with a beta-adrenergic receptor agonist. PDE4, rather than PDE3, appears to be responsible for modulating the amplitude and duration of the cAMP response to beta-agonists. PDE3 and PDE4 localize to distinct compartments and this may underpin their different functional roles. Our findings indicate the importance of distinctly localized PDE isoenzymes in determining compartmentalized cAMP signaling.
Cardiac myocytes have provided a key paradigm for the concept of the compartmentalized cAMP generation sensed by AKAP-anchored PKA. Phosphodiesterases (PDEs) provide the sole route for degrading cAMP in cells and are thus poised to regulate intracellular cAMP gradients. PDE3 and PDE4 represent the major cAMP degrading activities in rat ventriculocytes. By performing real-time imaging of cAMP in situ, we establish the hierarchy of these PDEs in controlling cAMP levels in basal conditions and on stimulation with a beta-adrenergic receptor agonist. PDE4, rather than PDE3, appears to be responsible for modulating the amplitude and duration of the cAMP response to beta-agonists. PDE3 and PDE4 localize to distinct compartments and this may underpin their different functional roles. Our findings indicate the importance of distinctly localized PDE isoenzymes in determining compartmentalized cAMP signaling.
There are two broad categories of COVID-19 tests on the market. The first category uses reverse transcriptase real-time polymerase chain reaction (RT-PCR) and nucleic acid hybridization strategies to identify viral RNA. Current FDA-approved diagnostic tests use this technique. Some drawbacks include the amount of time it takes to complete the test, the need for specialized personnel and the availability of equipment and reagents. The second category of tests focuses on the detection of antibodies. However, there could be a delay of a few days to a few weeks after a person has been exposed to the virus for them to produce detectable antibodies.. In recent years, researchers have had some success with creating point-of-care biosensors using 2D nanomaterials such as graphene to detect diseases. The main advantages of graphene-based biosensors are their sensitivity, low cost of production and rapid detection turnaround. The discovery of graphene opened up a new era of sensor development due to its ...
TY - JOUR. T1 - Simultaneous live cell imaging using dual FRET sensors with a single excitation light. AU - Niino, Yusuke. AU - Hotta, Kohji. AU - Oka, Kotaro. PY - 2009/6/24. Y1 - 2009/6/24. N2 - Fluorescence resonance energy transfer (FRET) between fluorescent proteins is a powerful tool for visualization of signal transduction in living cells, and recently, some strategies for imaging of dual FRET pairs in a single cell have been reported. However, these necessitate alteration of excitation light between two different wavelengths to avoid the spectral overlap, resulting in sequential detection with a lag time. Thus, to follow fast signal dynamics or signal changes in highly motile cells, a single-excitation dual-FRET method should be required. Here we reported this by using four-color imaging with a single excitation light and subsequent linear unmixing to distinguish fluorescent proteins. We constructed new FRET sensors with Sapphire/RFP to combine with CFP/YFP, and accomplished simultaneous ...