Biosensor technology is a powerful alternative to conventional techniques, harnessing the specificity and sensitivity of biological systems in small, low cost devices. Despite the promising biosensors developed in research laboratories, there are not many reports of applications in agricultural monitoring. The authors review biosensor technology and discuss the different bio-receptor systems and methods of transduction. The difference between a biosensor and a truly integrated biosensor system are defined and the main reasons for the slow technology transfer of biosensors to the marketplace are reported. Biosensor research and development has been directed mainly towards health care, environmental applications and the food industry. The most commercially important application is the hand-held glucose meter used by diaberics. The agricultural/veterinary testing market has seen a number of diagnostic tests but no true biosensor systems have made an impact. The need for fast, on-line and accurate ...
Ink-jet printing has been demonstrated as a manufacturing technique that facilitates the rapid, reproducible and economical production of amperometric glucose biosensors. Glucose was chosen as the analyte for demonstrating the process. For eight electrodes produced, the relative standard deviation of the response was less than 5%. The technique is extremely versatile, and will enable a wide variety of reagents to be placed on virtually any sensor design. This technique will be of particular benefit for the mass manufacture of intricate devices, where existing production techniques, such as screen-printing, may not be suitable.. ...
A biosensor system determines analyte concentration from an output signal generated by an oxidation/reduction reaction of the analyte. The biosensor system adjusts a correlation for determining analyte concentrations from output signals at one temperature to determining analyte concentrations from output signals at other temperatures. The temperature-adjusted correlation between analyte concentrations and output signals at a reference temperature may be used to determine analyte concentrations from output signals at a sample temperature.
A Rutgers-led team has created better biosensor technology that may help lead to safe stem cell therapies for treating Alzheimers and Parkinsons diseases and other neurological disorders. The technology, which features a unique graphene and gold-based platform and high-tech imaging, monitors the fate of stem cells by detecting genetic material (RNA) involved in turning such cells into brain cells (neurons), according to a study in the journal Nano Letters.
Antibacterials are among of the most important medications used in health care. However, their efficacy is increasingly impeded by a tremendous and globally spread bacterial resistance phenomenon. This bacterial resistance is accelerated by inadequate application of antibacterial drugs in humans, the widespread veterinary use of antibacterials, and antibacterial occurrence in the environment and food. Further, there is a lack of development of innovative novel drugs. Therefore, the search for novel antibacterials has to be intensified and the spread of antibacterials in the environment has to be restricted. Due to the fundamental progress in biosensor development and promising applications in the antibiotic field, this review gives for the first time an overview on the use and prospects of biosensor applications in that area. A number of reports have applied biosensors of different design and techniques to search for antibacterials in environmental and foodstuff matrices. These studies are discussed
In recent years, there has been rapidly growing interest in developing hand held, sensitive and cost-effective on-chip biosensing systems that directly translate the presence of certain bioanalytes (e.g., biomolecules, cells and viruses) into an electronic signal. The impressive and rapid progress in micro- and nanotechnology as well as in biotechnology enables the integration of a variety of analytical functions in a single chip. All necessary sample handling and analysis steps are then performed within the chip. Microfluidic systems for biomedical analysis usually consist of a set of units, which guarantees the manipulation, detection and recognition of bioanalytes in a reliable and flexible manner. Additionally, the use of magnetic fields for performing the aforementioned tasks has been steadily gaining interest. This is because magnetic fields can be well tuned and applied either externally or from a directly integrated solution in the biosensing system. In combination with these applied magnetic
Ms. Witz led GE Healthcares Healthcare Systems Interventional global business, which researches and develops innovative medical technologies to help physicians diagnose and treat disease earlier, faster and more accurately. "Leading the GE Medical Diagnostics team is an honor," said Witz. "Im eager to continue the mission of advancing medicine through novel diagnostic agents. We will raise the bar in diagnosing critical diseases such as Parkinsons disease, Alzheimers and heart failure.". "Pascale has a breadth of experience that makes her an ideal person to lead the Medical Diagnostics business," said Dineen. "She brings an extensive background in molecular biology as well as imaging, both of which are critical to driving Medical Diagnostics development of its portfolio of innovative molecular diagnostic imaging agents.". For more information: www.gehealthcare.com. ...
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Biosensors are devices that use biological components to detect important analytes. Biosensing systems have various applications in areas such as medicine, environmental monitoring, and process control. Classical biosensors are often based on bacteria or purified enzymes that have limitations on efficiency or stability. I have developed several new biosensors to overcome these disadvantages. Two preliminary biosensors were first created based on the extremely strong and specific interaction between biotin and (strept)avidin. Both biosensors showed high sensitivity and reliability for measuring biotin with detection limits of 50-1000 pg/ml and 20-100 ng/ml, respectively. Following these, a new biosensor was developed by coupling a mobile, functionalized microsurface with cell-free expression approaches. This biosensor demonstrated a dynamic range of 1- 100 ng/ml. In addition, I also explored the possibility of combining these biosensing systems with engineered living cells. By leveraging the ...
To boost our understanding of Taspase1s substrate specificity we used our biosensor assay mixed with positional scanning mutagenesis
Biosensors are poised to make a large impact in environmental, food, and biomedical applications, as they clearly offer advantages over standard analytical methods, including minimal sample preparation and handling, real-time detection, rapid detection of analytes, and the ability to be used by non-skilled personnel. Covering numerous applications of biosensors used in food and the environment, Portable Biosensing of Food Toxicants and Environmental Pollutants presents basic knowledge on biosensor technology at a postgraduate level and explores the latest advances in chemical sensor technology for researchers. By providing useful, state-of-the-art information on recent developments in biosensing devices, the book offers both newcomers and experts a roadmap to this technology. In the book, distinguished researchers from around the world show how portable and handheld nanosensors, such as dynamic DNA and protein arrays, enable rapid and accurate detection of environmental pollutants and pathogens. ...
Fluorescent glucose biosensors are devices that measure the concentration of glucose in diabetic patients by means of sensitive protein that relays the concentration by means of fluorescence, an alternative to amperometric sension of glucose. No device has yet entered the medical market, but, due to the prevalence of diabetes, it is the prime drive in the construction of fluorescent biosensors. Keeping glucose levels in check is crucial to minimize the onset of the damage caused by diabetes. As a consequence, in conjunction with insulin administrations, the prime requirement for diabetic patients is to regularly monitor their blood glucose levels. The monitoring systems currently in general use have the drawback of below optimal number of readings, due to their reliance on a drop of fresh blood. Some continuous glucose monitors are commercially available, but suffer from the severe drawback of a short working life of the probe. The majority of these work amperometrically. As a result, there is ...
Books Biofunctionalization of Nanomaterials Nanotechnologies for the Life Sciences, Vol. 1 Edited by Challa Kumar. Wiley-VCH, Weinheim 2005. 425 pp., hardcover E 139.00.-ISBN 3-527-31381-8 With this first volume, Biofunctionalization of Nanomaterials, Wiley-VCH and the editor, Challa Kumar, introduce a series that has an ambitious aim. The plan is for a ten-volume encyclopedia that will collect together and critically evaluate all aspects of the current state of knowledge about the use of nanotechnology in the life sciences. This is certainly one of the most dynamic areas of research at present, and therefore to attempt a comprehensive summary and evaluation is a challenging task. Moreover, this field has the distinction that, perhaps more than any other current area of research, it has inputs from chemistry, biology, biochemistry, materials science, and physics. Therefore, it is important that the knowledge must be communicated in a way that extends beyond the boundaries of the classical ...
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A new approach is presented to obtain fluorescent sensors for pH windows that work in water and under biomimetic conditions. A single molecule that features all-covalently linked components is used, thus making it capable of working as a fluorescent sensor with an OFF/ON/OFF response to pH value. The components are a tertiary amine, a pyridine, and a fluorophore (pyrene). The forms with both protonated bases or both neutral bases quench the pyrene fluorescence, whereas the form with the neutral pyridine and protonated amine groups is fluorescent. The molecular sensor is also equipped with a long alkyl chain to make it highly hydrophobic in all its protonated and unprotonated forms, that is, either when neutral or charged. Accordingly, it can be confined at any pH value either in traditional (i.e., low-molecular-weight) nonionic surfactant micelles or inside polymeric, biocompatible micellar containers. Relevant for future applications in vivo, thanks to its strong hydrophobicity, no leakage of ...
The aim of this work is the development of a cost effective, rapid, portable analysis system for the measurement of bioavailable toxic metal or organic contaminants, which may be present at natural attenuation landfill sites requiring remediation. The proposed system is intended to be used as a tool in the monitoring or characterisation of a site by discrete measurement. A portable system will be developed, comprising a miniaturised extraction and separation device employing capillary electrophoresis, detectors for specific analyses (metals or organics) by biosensors or immunosensors and dedicated instrumentation to process the signals generated. Development of extraction procedures for the analysis of plant material will also be part of the project, to be used when phytoremediation is employed as part of the site remediation process. Biosensors to detect toxic metal will use bioengineered proteins to interact with bioavailable metals on an electrode surface. The conformational change in the
We describe a novel biosensor system for reporting proximity between cell surface proteins in live cultured cells. The biosensor takes advantage of recently developed fluorogen-activating proteins (FAPs) that display fluorescence only when bound to otherwise-nonfluorescent fluorogen molecules. To demonstrate feasibility for the approach, two recombinant rapamycin-binding proteins were expressed as...
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Neural Networks for an Analysis of the Hemometabolites Biosensor Response: 10.4018/ijehmc.2013100106: In this work, the concentration dependent response of amperometric biosensor array for the biomarkers glucose, cholesterol and urease was explored, using
Contamination of drinking water with arsenic is a recurring problem in both industrialized and developing countries. Supplies for large populations can have concentrations much higher than the permissible levels, set by the World Health Organization (WHO) to 10 μg As/L for most European countries and the United States and to 50 μg As/L elsewhere. As arsenic analysis requires high-end instruments, which are largely unavailable in developing countries, bioassays based on genetically engineered bacteria have been proposed as suitable alternatives. Yet, such tests would profit from better standardization and direct incorporation into sensing devices. The final objective of this work was to develop a microfluidic device in which bacterial bioreporters could be embedded, actively maintained for at least one week, exposed to arsenic and which allowed direct detection of the reporter signal produced, as a further step towards a complete miniaturized bacterial biosensor. The signal element in the biosensor is
The importance of nanotechnology in medical technologies, especially biomedical diagnostics, is indubitable. By taking advantages of nanomaterials, many medical diagnostics methods have been developed so far, including electrochemical nanobiosensors. They have been used for quantification of different clinical biomarkers for detecting, screening, or follow up a disease. microRNAs (miRNAs) are one of the most recent and reliable biomarkers used for biomedical diagnosis of various diseases including different cancer types. In addition, there are many electrochemical nanobiosensors explained in publications, patents, and/or a commercial device which have been fabricated for detection or quantification of valuable miRNAs. The aim of this article is to review the concept of medical diagnostics, biosensors, electrochemical biosensors and to emphasize the role of nanotechnology in nanobiosensor development and performance for application in microRNAs detection for biomedical diagnosis. We have also summarized
The global Glucose Biosensor Market is anticipated to witness lucrative growth during the period of 2015 to 2022. The homecare diagnostics Glucose Biosensor Market is projected to flourish over the forecast period. Ease-of-use, quick results, and low costs of the tests are some aspects supporting market growth. On the other hand, the hospital-based Glucose Biosensor Market is projected to grow at a significant CAGR of 10.3% during the period 2015 to 2022.. Browse Detail Report With TOC @ http://www.hexareports.com/report/glucose-biosensor-market/details. Some key factors attributing to the growth of this market include the increasing incidence of diabetes across the globe, development of technologically advanced products, growing demand for minimally and non-invasive products, patient awareness levels, and the need for early diabetes detection. However, presence of other alternatives for management of the diabetes, such as laboratory methods is expected to restrict market growth.. Request A ...
Analysis of biological components is central in bioprocess monitoring, process control, product quality control and cell based toxicity assaying. One of these themes that is pursued in this thesis is the use of biosensors for monitoring of molecular markers, exploiting the natural selectivity of biomolecules. Another is the use of glycoconjugates to monitor the activity of biomolecules in a flu vaccine process is studied and were the sensor is based on the concept of weak affinity giving fast response time for the sensor.. A third theme is monitoring of cell cultures used for toxicity testing different protein markers is of interest.. When developing biosensor surfaces for new antigens commercial preparations of antibodies are often used. In this work we have chosen to look at lactate dehydrogenase (LDH) and describe the preparation and characterisation of antibody used in biosensor surface development.. The design of a sensor surface is important for the characteristics of a sensor. By binding ...
Genetically-encoded fluorescent sensor proteins are attractive tools for studying intracellular Zn2+ homeostasis and signaling. Here we provide an overview of recently developed sensors based on Förster Resonance Energy Transfer (FRET). The pros and cons of the various sensors are discussed with respect to Z Iron and zinc sensing in cells and the body
The Nova StatStrip Glucose Hospital Meter System (StatStrip) and the StatStrip Xpress2 Glucose Hospital Meter System (StatStrip Xpress2) are the only glucose analyzers cleared by the FDA for use in all types of hospital patients, including critically ill patients receiving intensive medical intervention. For healthcare settings other than critical care, StatStrip and StatStrip Xpress2 are cleared for use with capillary finger stick, arterial, venous, neonatal heel stick and neonatal arterial specimens. When using the system to measure glucose on critically ill patients, StatStrip and StatStrip Xpress2 are cleared for use with arterial, venous, neonatal heel stick and neonatal arterial specimens. StatStrip and StatStrip Xpress2 are not cleared for capillary specimens on patients receiving intensive medical intervention/therapy because capillary samples from these patients, particularly those with decreased peripheral blood flow, may not truly reflect a patients physiological state. Some ...
Researchers at Harvard University have developed a non-toxic wearable sensor consisting of a silicon-rubber that attaches to the hand and capable of mearin | Technology
Waltham, MA, January 2011 - Nova Biomedical today announced that, in response to rapid growth in its diabetes and whole blood point-of-care testing products business, it has purchased an additional 80,000 square-foot manufacturing/warehouse facility in Billerica, MA. According to Lou Borrelli, Nova Biomedical CFO, "This additional state of the art manufacturing facility will ensure that our manufacturing capabilities keep pace with the increasing demand for our StatStrip Hospital Glucose products as well as our Nova Max consumer diabetes products.". One of the main drivers for Novas strong growth is the rapid adoption of its StatStrip Hospital Glucose Monitoring System. Since its inception just four years ago, StatStrip has become the fastest growing hospital glucose meter in the world. StatStrip uses a novel glucose test strip technology that measures hematocrit and other common interferences such as maltose, galactose, xylose, acetaminophen, ascorbic acid and oxygen, and eliminates erroneous ...
The most rapid and reliable way of detecting HIV is to search for the HIV genetic material directly in blood, using a process called PCR (polymerase chain reaction). This has the advantage of not needing to wait for such a long time after exposure, being used after only 3 days. Traditionally this process, known as molecular diagnostics, has been performed in a laboratory by trained personnel. Recent technological advances have however brought the possibility of conducting molecular diagnostics out of the laboratory and next to the patient at the point-of-care ...
Boyce Thompson Insitute scientist Maria Harrison will collaborate with Wayne Versaw of Texas A&M University to create a new imaging system to visualize and measure the movement of phosphate from soil fungi into plant root cells in a $1.2 million DOE-funded project.
TY - JOUR. T1 - A contour-mode film bulk acoustic resonator of high quality factor in a liquid environment for biosensing applications. AU - Xu, Wencheng. AU - Choi, Seokheun. AU - Chae, Junseok. PY - 2010/2/17. Y1 - 2010/2/17. N2 - This letter reports an acoustic resonator of high quality factors (Qs) operating in liquid media. The film bulk acoustic resonator (FBAR) is made of a ring-shaped piezoelectric aluminum nitride thin film, and is excited in a contour mode. By having a low motional resistance upon coupling with liquids, the contour mode FBAR achieved Qs up to 189, more than 12× over the state-of-the-art FBARs in liquids. The resonator was characterized by an aptamer-thrombin binding pair for a biosensor and showed a mass resolution of 1.78 ng/ cm2.. AB - This letter reports an acoustic resonator of high quality factors (Qs) operating in liquid media. The film bulk acoustic resonator (FBAR) is made of a ring-shaped piezoelectric aluminum nitride thin film, and is excited in a contour ...
This thesis treats the development of an integrated optical sensor array. The sensors are slot-waveguide ring resonators, integrated with on-chip surface grating couplers and light splitters, for alignment tolerant, real-time, refractive index sensing, and label-free biosensing. The work includes: the design of components and system layouts, the development of fabrication methods, the fabrication of sensor chips, the characterization of the chips, and the development of physical system models for accurate extraction of resonance wavelengths in measured spectra.. The main scientific achievements include: The evaluation of a novel type of nano-structured optical waveguide for biochemical sensing. The realization of an array of such slot-waveguide sensors, integrated with microfluidic sample handling, for multiplex assays. The first study of the thermal behavior of slot-waveguide sensors and the discovery of unique temperature compensation capabilities.. From an application perspective, the use of ...
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Environmental Applications of Nanoscale and Microscale Reactive Metal Particles takes the reader from current laboratory studies in the function and use of these particles through scale-up and finally to the state-of-the-science in field-scale demonstrations and applications.
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
Cornings benchtop label-free detection system features a compact design, a more affordable price point, simple data collection software, and whole microplate analysis using charge-coupled device (CCD) detection of wavelength shift. This innovative new system is designed to improve your assay capabilities and allows researchers, including academic laboratories, easy access to the Epic label-free detection technology.. ...
A method for determining one member of an enzyme-substrate pair (the analyte), comprises bringing the members of the pair into contact so as to form, directly or indirectly, a soluble reaction product at or adjacent the surface of an optical waveguide biosensor. The biosensor is preferably a resonant optical biosensor based on the principle of frustrated total reflection.
A solid-state electrochemiluminescence (ECL) switch biosensor using Ru(bpy)32+/β-cyclodextrin-Pd nanoparticles (β-CY-PdNPs)/Gelatin (Gel) complex and ferrocene-labeled DNA probe (Fer-DNA) for the detection of Hg2+ was successfully developed. The ECL biosensor includes an ECL substrate and an ECL signal switc
John Fourkas, a chemistry professor at the University of Maryland, and his research team have developed materials and nanofabrication techniques to build miniaturized versions of components needed for sensors, medical diagnostics and other applications.. This approach allows for faster analysis at a lower cost with small sample volumes. The materials provide simultaneous 3D manipulation of microscopic objects using optical tweezers and a unique method for lithography, which is the process of using light in etching silicon or other substrates to create electronic components. These techniques allow complex 3D structures to be created from many microscopic components.. Previous work by this UMD research team found a breakthrough in the use of visible light for making small structures for applications including optical communications, cell behavior control and the manufacturing of integrated circuits.. Fourkas said, "One of the exciting aspects of this set of techniques is that it is compatible with ...
Title:Sol-Gel Chemistry in Biosensing Devices of Electrical Transduction: Application to CEA Cancer Biomarker. VOLUME: 15 ISSUE: 3. Author(s):Liliana A.A.N.A. Truta and M. Goreti F. Sales. Affiliation:BioMark Sensor Research, Instituto Superior de Engenharia do Porto, R. Dr. Antonio Bernardino de Almeida, 431, 4200-072 Porto, Portugal.. Keywords:Antibody, Biosensors, Cancer Biomarker, CEA, Sol-gel.. Abstract:Sol-gel chemistry allows the immobilization of organic molecules of biological origin on suibtable solid supports, permitting their integration into biosensing devices widening the possibility of local applications. The present work is an application of this principle, where the link between electrical receptor platform and the antibody acting as biorecognition element is made by sol-gel chemistry. The immunosensor design was targeted for carcinoembryonic antigen (CEA), an important biomarker for screening the colorectal cancer, by electrochemical techniques, namely electrochemical impedance ...
Sarvazyan, A.P. (1998) Mechanical Imaging A New Technology for Medical Diagnostics. International Journal of Medical Informatics, 49, 195-216.
Generally, an immunoaffinity SPR biosensor detects a target analyte in a sample through highly selective adsorption by using the antigen–antibody interaction. For improving the sensitivity, various kinds of particles have been added to the already bound analytes on the SPR biosensor (sandwich assay). In this work, signal amplification was demonstrated by the expression of the IgG-binding Z-domain of protein A on the outer membrane of Escherichia coli via "Autodisplay". The amount of Z-domain of protein A expressed on the outer membrane was calculated to be 280,000 molecules per cell. In addition, the IgGbinding ability of the expressed proteinwas characterized using FACS analysis. The signal amplification of the SPR biosensor was performed in the sandwich assay format using a model of horseradish peroxidase (HRP); the limit of detectionwas determined to be significantly improved from1 g/ml to 1 ng/ml. Finally, myoglobin analysis was demonstrated for the medical diagnosis of cardiac ...
Biomoda granted Mexican patent for proprietary porphyrin-based compound Cancer diagnostics organization Biomoda, Inc. porphine, or TCPP, may be the foundation for many Biomoda products and medical diagnostics. Email address details are in comparison to CT Pap and scans spots examine by independent radiologists and cytopathologists, respectively, to confirm precision. Biomoda is seeking Meals and Medication Administration acceptance of its cytology-centered screening technology as a Course III medical gadget..Patients with any registration of a congenital heart defect and the ones with coronary disease diagnosed at ...
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We report on results achieved with three different types of polymer-coated chemical microsensors fabricated in industrial CMOS technology. The first and most extensively studied transducer is a microcapacitor sensitive to changes in dielectric properties of the polymer layer due to analyte absorption. An on-chip integrated (Sigma) (Delta) -converter allows for detecting the minute capacitance changes. The second transducer is a resonant cantilever sensitive to predominantly mass changes. The cantilever is electrothermally excited, its vibrations are detected using a piezoresistive Wheatstone bridge. In analogy to acoustic wave devices, analyte absorption in the polymer causes resonance frequency shifts as a consequent of changes in the vibrating mass. The last transducer is a microcalorimeter consisting of a polymer-coated sensing thermopile and an uncoated reference thermopile each on micromachined membranes. The measurand is the absorption or desorption heat of organic volatiles in the polymer ...
The potential uses of smell technology are endless," notes Josep Samitier, the coordinator of the SPOT-NOSED project that developed nanobiosensors to mimic the way human and animal noses respond to different odours. This new nose biosensor is unusual in how its made. By placing a layer of proteins that constitute the olfactory receptors in animal noses on a microelectrode and measuring the reaction when the proteins come into contact with different odorants, the system is capable of detecting odorants at concentrations that would be imperceptible to humans.. "Our tests showed that the nanobiosensors will react to a few molecules of odorant with a very high degree of accuracy. Some of the results of the trials surpassed even our expectations," Samitier says. These tiny bioelectronic sensors, he says, represent a major leap forward in smell technology and a clear example of a biomimetic devices obtained by converging Nano-Bio-Info technologies.. Several hundred different proteins, which the ...