Using published dairy cattle liver transcriptomics dataset along with novel blood biomarkers of liver function, metabolism, and inflammation we have attempted an integrative systems biology approach applying the classical functional enrichment analysis using DAVID, a newly-developed Dynamic Impact Approach (DIA), and an upstream gene network analysis using Ingenuity Pathway Analysis (IPA). Transcriptome data was generated from experiments evaluating the impact of prepartal plane of energy intake [overfed (OF) or restricted (RE)] on liver of dairy cows during the peripartal period. Blood biomarkers uncovered that RE vs. OF led to greater prepartal liver distress accompanied by a low-grade inflammation and larger proteolysis (i.e., higher haptoglobin, bilirubin, and creatinine). Post-partum the greater bilirubinaemia and lipid accumulation in OF vs. RE indicated a large degree of liver distress. The re-analysis of microarray data revealed that expression of ,4,000 genes was affected by diet x ...
The ARIC (Atherosclerosis Risk in Communities) study recruited a population-based cohort of 15,792 persons between 45 and 64 years of age in 1987-1989, from Forsyth County, NC, and Jackson, MS. In the ARIC study cohort, the investigators measured multiple blood biomarkers of inflammation, hemostasis, thrombin generation, cardiac dysfunction, and vascular stiffness, and identified incident AAAs during follow-up using hospital discharge codes. The authors performed multivariate modeling to obtain hazard ratios of incident clinical AAA in relation to biomarker quartiles by use of Cox proportional hazards regression.. ...
This trial will investigate the effiects of nintedanib [OFEV; Boehringer Ingelheim] on chnages in specific blood biomarkers that can predict course of
In this post-hoc analysis of a subcohort of a large, multicenter prospective cohort study of cardiac surgery patients without creatinine-based acute kidney injury, we did not find evidence of association between either pre- or postoperative biomarker levels and age or sex. However, there were significant differences in biomarker levels associated with urine dipstick findings, including proteinuria, hematuria, leukocyte esterase and nitrites.. All biomarkers were mildly correlated with hematuria, indicating that blood might interfere with biomarker levels or their measurement even in the absence of overt kidney injury evidenced by creatinine rise. Preexisting substances in the urine interfering with assays for novel analytes has been previously described. [10]. We found evidence that proteinuria is associated with all four biomarkers both pre- and post-operatively. Dipstick proteinuria is a marker of preexisting kidney damage and is associated with both the incidence and outcomes of acute kidney ...
Cardiac markers are biomarkers measured to evaluate heart function. They are often discussed in the context of myocardial infarction, but other conditions can lead to an elevation in cardiac marker level. Most of the early markers identified were enzymes, and as a result, the term "cardiac enzymes" is sometimes used. However, not all of the markers currently used are enzymes. For example, in formal usage, troponin would not be listed as a cardiac enzyme. Measuring cardiac biomarkers can be a step toward making a diagnosis for a condition. Whereas cardiac imaging often confirms a diagnosis, simpler and less expensive cardiac biomarker measurements can advise a physician whether more complicated or invasive procedures are warranted. In many cases medical societies advise doctors to make biomarker measurements an initial testing strategy especially for patients at low risk of cardiac death. Many acute cardiac marker IVD products are targeted at nontraditional markets, e.g., the hospital ER instead ...
The results of this study show that changes in numbers of synovial sublining macrophages correlate with clinical improvement independently of the therapeutic strategy. Furthermore, this study demonstrates that the change in the numbers of sublining macrophages may be used to explain clinical outcome. Of importance, the data indicate that the change in the number of sublining macrophages could be used as a sensitive biomarker to predict possible efficacy of a new antirheumatic treatment.. Previous work suggested an association between the number of synovial macrophages and joint destruction in RA.14 Moreover, analysis of the synovial cell infiltrate demonstrated a positive correlation between scores for local disease activity and the number of macrophages as well as expression of macrophage derived cytokines (tumour necrosis factor α (TNFα) and interleukin (IL) 6) in rheumatoid ST, suggesting that macrophage numbers are associated with clinical signs of inflammation.2 In keeping with this ...
SirYus technology enables sensitive and reproducible tests for cancer screening by combining several biomarkers to establish an appropriate diagnosis.. Considerable efforts have been devoted to search specific markers with relatiely few results. In fact, from 1261 proteins identified as potentially discriminatory in human cancers, only nine have received final approval from the FDA as biomarkers of tumors.. Many opinion leaders now believe that the pursuit of individual biomarkers will be vain, and that several biomarkers are needed to properly identify cancers in screening protocols.. SirYus multiplex technology brings new solutions to this complex challenge. Simultaneous testings of several biomarkers can largely improve screening sensitivity and specificity.. ...
In clinical practice, biomarkers can be used to identify risk and susceptibility, diagnose a disease, assess disease severity or progression, classify patients, guide treatment, and predict prognosis. In drug development and the pharmaceutical industry, biomarkers can be used to predict toxicity, safety, or efficacy of a drug. Biomarkers can be categorized as target, mechanism and clinical to indicate if a drug hits its intended target, alters any mechanisms and if it is effective in vivo. Biomarkers can be also classified into three types: type 0 - natural history markers, type 1 - biological or drug activity markers, and type 2 - surrogate markers. Type 0 biomarkers measure the natural history of a disease and should correlate over time with known clinical indicators. They can be characterized in phase 0 clinical trials. Symptoms over the full range of a disease and most prognosis markers are type 0 biomarkers. In most cases, type 1 biomarkers are the markers that capture the effects of a ...
CSDE is offering a hands-on workshop in biomarker methods that provide quick on-site measurements in non-clinical settings without the need to transport samples back to a lab. Well practice proper technique for minimally invasive measures like height, weight, body composition, and blood pressure, and the use of simple tools for biomarker testing with blood collected from a finger prick. A brief lecture component will cover costs, logistics, human subjects implications, and strengths and limitations of this approach to biomarker data collection. Please email Ellie Brindle ([email protected]) to register.. ...
Attempts to find a lone biomarker for Alzheimers disease - whether its in blood, spinal fluid, or the brain - have largely failed. The Texas Alzheimers Research Consortium project conducted a longitudinal case-control study, using stored blood samples to develop an algorithm that separates patients with Alzheimers disease from controls. The biomarker assays looked at hundreds of proteins, including thrombopoietin, TNF-alpha, creatine kinase, and various interleukins. The his team focused on a large array of blood-based proteins, since assay technology has now made it possible to evaluate large amounts of data. Screening for these biomarkers and factoring in age, sex, education, and APOE status led to a sensitivity of 0.94 and a specificity of 0.84, as reported by Sid OBryant, PhD, of Texas Tech University, in Lubbock, Texas, and colleagues in the Archives of Neurology. They also saw that many of the proteins with the highest importance were inflammatory in nature, which suggests that the ...
In this study, biomarker response after supplementation with oral and intramuscular vitamin B12 will be compared in a randomized clinical trial. Electronic compliance monitoring will be used to control for non compliance as a possible confounder in oral treatment. Additionally subjective acceptance in terms of presumed preferences will be compared with oral vs. intramuscular supplementation of vitamin B12 in the view of the patient ...
A diagnostic study of 136 premature infants found that a protein involved in managing harmful bacteria in the human intestine is a reliable biomarker for the noninvasive detection of necrotizing enterocolitis.
Purdue University researchers have found a method of identifying biological markers in small amounts of blood that they believe could be used to detect a myriad of diseases, infections and different medical conditions at early stages.
Hypothesis: The association of excess weight with an adverse cardiometabolic profile in patients with type 1 diabetes (T1D) is unclear. The purpose of this study was to test the hypothesis that increasing BMI and adiposity indicators in youth with T1D are adversely associated with glycemic control and cardiovascular biomarkers.. Methods: Subjects were youth participants of a family-based randomized controlled dietary intervention (N=136, age=12.3±2.5y, baseline A1c=8.1±1.1%). Glycemic control (A1c and 1,5-Anhydroglucitol, 1,5-Ag), body mass index (BMI, from measured height and weight), serum lipids (total cholesterol, TC; HDL-cholesterol, HDL-C; LDL-cholesterol, LDL-C; triglycerides, TG), inflammation (c-reactive protein, CRP), oxidative stress (8-iso-prostaglandin F2alpha, 8-iso-PGF2α), adiponectin and blood pressure (systolic, SBP; diastolic, DBP) were assessed at baseline and every 6 months for 18 months. Total and truncal lean, fat-free mass and percent fat (%fat) were measured by Dual ...
Nivolumab response rate is 40% in metastatic melanoma. Few studies have evaluated pre-treatment biomarkers predictive of response. The aim of this study was to identify potential peripheral blood biomarkers associated with survival in patients with advanced melanoma treated with nivolumab. All advanced melanoma cases treated with anti-programmed cell death protein 1 (anti-PD1) over a 3-year period in our department were identified. For each case, 9 potential blood biomarkers were identified. Bivariate and multivariate analyses, adjusted for the American Joint Committee on Cancer (AJCC) classification stage, Eastern Cooperative Oncology Group (ECOG) performance status, lactate dehydrogenase (LDH) level and failure to respond to first-line therapy, were used to test the association between biomarkers and overall survival (primary outcome) or progression-free survival (secondary outcome ...
Biomarkers in the bloodstream could provide a rapid assessment of therapeutic intervention in ischemic stroke and allow patient stratification Researc
ESMO) 2016 Congress. The presentation included interim efficacy and safety outcomes, as well as initial findings from the companys ongoing biomarker analysis of samples collected during the study. The SUNRISE Phase III trial was discontinued earlier this year based on a pre-specified interim analysis although patient treatment and follow-up in the study were allowed to continue. The pre-planned biomarker analysis has been taking place as patient follow-up has continued and available results were evaluated as part of the recent top-line data analysis. The study protocol pre-specified the collection of thousands of patient samples for exploratory analyses over a wide range of possible biomarkers, including pre-treatment levels of beta-2 glycoprotein-1 (β2GP1). Data presented at ESMO demonstrated that patients with pre-treatment β2GP1 levels between 200 and 240 (representing approximately 30% of randomized patients) achieved a statistically significant, 5.5-month improvement (13.2 months vs. 7.7 ...
Our literature search identified 93 studies evaluating an overall of 70 different blood markers for CRC, including long discussed ones like carcinoembryonic antigen and carbohydrate antigens, as well as newly introduced ones like proteins identified through MS analysis. A broad timeframe with first studies from 1979 (75) to 2006 (49, 73, 74, 77, 81, 89-91, 102) was covered. The majority of studies evaluated protein markers, but in recent years, an interesting number of studies also evaluated genetic and epigenetic markers.. Overall, a broad range of sensitivity and specificity was reported for the various markers. A direct comparison of results from different studies is complicated due to the diverse populations used (different age, origin, "normal," or diseased controls), the diverse number of markers evaluated (single versus combined markers) and use of different cutoff points for the same marker (M2-PK and CEA). Furthermore, the majority of markers were evaluated in only one study, especially ...
Alzheimer disease biomarker testing in cerebrospinal fluid : a method to harmonize assay platforms in the absence of an absolute reference standard ...
Serological and proteomic biomarkers can help clinicians diagnose rheumatic diseases earlier and assess disease activity more accurately. These markers have been incorporated into the recently revised classification criteria of several diseases to enable early diagnosis and timely initiation of treatment. Furthermore, they also facilitate more accurate subclassification and more focused monitoring for the detection of certain disease manifestations, such as lung and renal involvement. These biomarkers can also make the assessment of disease activity and treatment response more reliable. Simultaneously, several new serological and proteomic biomarkers have become available in the routine clinical setting-for example, a protein biomarker panel for rheumatoid arthritis and a myositis antibody panel for dermatomyositis and polymyositis. This review will focus on commercially available antibody and proteomic biomarkers in rheumatoid arthritis, systemic lupus erythematosus, systemic sclerosis ...
Serum cytokeratin-18 is a non-invasive biomarker for evaluating disease severity in patients with liver cirrhosis and hepatocellular carcinoma
Semantic Scholar extracted view of Dynamic changes in calprotectin and its correlation with traditional markers of oxidative stress in patients with acute ischemic stroke. by Antonios Chatzopoulos et al.
Biomarker classification depends on its context of use, which in turn defines the extent to which the biomarker must be "validated." In terms of requirements for validation, the "holy grail" of biomarker application is one that can serve as a surrogate endpoint in a clinical trial. A surrogate endpoint is a laboratory (or physical) measurement that is used in a clinical trial as a substitute for a clinical endpoint (e.g., survival or functional endpoint). Therefore, a surrogate endpoint must accurately predict the effect of therapeutic intervention. Guidance for Industry: Pharmacogenomic Data Submissions. The use of "companion diagnostics" to drive molecularly targeted drug development also requires that the "diagnostic" be clinically validated through the FDAs PMA or 510(K) process. In both of these instances the biomarker must be analytically validated (inclusive of the test platform, reagents, etc.).. The subtle differences between biomarkers, diagnostics, surrogate endpoints and companion ...
This study has been designed to evaluate a new oral test for therapeutic monitoring of HIV/AIDS patients that are receiving the combination Anti-Retroviral Therapy (cART). The test will measure saliva-based Stress Response Profiling(SRP) biomarkers using laboratory assays. Results of the test will show if HIV/AIDS patients successfully responded to cART. Preliminary studies showed that SRP biomarkers were strongly increased in cART-unresponsive AIDS patients. However, the diagnostic accuracy of the oral test, patients will be recruited to donate saliva: AIDS patients responsive or unresponsive to cART, and controls (acute or early HIV patients, and HIV-negative patients with hepatitis).. The saliva samples will be used to measure SRP biomarker concentrations. Results will show whether the biomarker measurements provide accurate and specific diagnostics for ART response. ...
This paper addresses the problem of classifying cells expressing different biomarkers. A deep learning based method that can automatically localize and count the cells expressing each of the different biomarkers is proposed. To classify the cells, a Convolutional Neural Network (CNN) was employed. Images of Immunohistochemistry (IHC) stained slides that contain these cells were digitally scanned. The images were taken from digital scans of IHC stained cervical tissues, acquired for a clinical trial. More than 4,500 RGB images of cells were used to train the CNN. To evaluate our method, the cells were first manually labeled based on the expressing biomarkers. Then we performed the classification on 156 randomly selected images of cells that were not used in training the CNN. The accuracy of the classification was 92% in this preliminary data set. The results have shown that this method has a good potential in developing an automatic method for immunohistochemical analysis ...
Normally distributed continuous variables were compared with the Student t test and skewed-distributed with the Wilcoxon rank-sum test. Categorical variables were compared with the chi-square test. The prognostic value of the biomarkers was assessed by investigating the relationship between mortality and biomarkers in 2 sets of Cox proportional-hazards analyses: univariate analyses of single biomarkers and multivariate analysis including all biomarkers (multimarker). The univariate analyses were used to determine cutoff values for the biomarkers. These cutoff values were based on the quartiles of the individual biomarker levels. When hazard did not differ significantly between adjacent quartiles, as indicated by the p value from the Cox model, these were merged.. A multivariate model initially was developed incorporating the 5 biomarkers. Biomarkers with p < 0.05 by the Wald test were identified by backward selection. From the final model, a simplified score was obtained by putting weights to ...
Learn about the veterinary topic of Other Serum Biochemical Measures in Hepatic Disease in Small Animals. Find specific details on this topic and related topics from the Merck Vet Manual.
Press Release issued Jul 8, 2014: Biomarkers also known as biological markers are characteristics that are measured in order to assess and predict biological condition of the body and thus help physicians in clinical decision making. Some major diseases which are diagnosed by using biomarkers include diabetes, cancer and cardiovascular and central nervous system diseases. Biomarkers play critical role in identifying and accurate diagnosis of these diseases at early stage and provide better treatment option. Imaging biomarkers can be classified as diagnostic biomarkers, monitoring biomarkers and predictive biomarkers. Diagnostic biomarkers are helpful in predicting a patients exact clinical outcome. Monitoring biomarkers provides clinical outcome of a patient after intake of a particular drug while predictive biomarkers help in predicting if a specific drug is likely to change clinical outcome of a patient.
The dependence of tumor growth and metastasis on blood vessels makes tumor angiogenesis a rational target for therapy. Strategies have been pursued to inhibit neovascularization and to destroy existing tumor vessels, or both. These include direct targeting of endothelial cells, and indirect targeting by inhibiting the release of proangiogenic growth factors by cancer or stromal cells. Many patients benefit from antiangiogenic therapies; thus, development of noninvasive biomarkers of disease response and relapse is a crucial objective to aid in their management. A number of non-invasive tools are described with their potential benefits and limitations. We review currently available candidate biomarkers of anti-angiogenic agent effect. Including these markers into clinical trials may provide insight into appropriate dosing for desired biological effects, appropriate timing of additional therapy, and prediction of individual response. This has important consequences for the clinical use of angiogenesis
The Applied Biosystems TaqMan Advanced Serum/Plasma 96-Well Plates, Standard, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well standard plate format. This product contains a set of 2 plates for profiling of up to 188 unique miRNAs in serum/plasma samples, and includes
In [11], we first proposed an efficient algorithm, AMFES (Adaptive Multiple FEatues Selection), to select important biomarkers for cancers. Based on that initial success, this paper reports the extension of previous results on the datasets provided by Maes et al. in an attempt to discover important biomarkers for AD from the blood-based samples [12]. Unlike traditional statistical analyses, AMFES is an SVM-based methodology, which can select a much smaller subset of important biomarkers. In addition, AMFES applies an adaptive method which enables selection of a globally optimal subset of important biomarkers compared to SVM-RFE. AMFES is particularly useful for differentiating noisy biomarkers from the relevant ones when interferences between biomarkers exist. Our results are supported by a high ROC/AUC (Receiver Operating Characteristic/Area Under Curve) value when we apply a cross-validation verification. Thus, AMFES should play an important role in the classification framework of ...
Press Release issued Apr 25, 2014: Cardiovascular diseases are leading cause of morbidity and mortality globally. Biomarkers are generally used as an indicator forparticular diseases such as rheumatic and congenital heart diseases and cardiac arrhythmia. Cardiac biomarkers are measurable and quantifiable biological parameters. These are specifically used to check the presence or severity of cardiovascular diseases. Cardiac biomarkers are the substances that are released into blood when heart is damaged. These biomarkers are also used for diagnosing and monitoring diseases such as coronary syndrome or cardiac ischemia. The symptoms of these diseases includes chest pain, shortness of breath and nausea that ultimately leads to heart attack or angina. Cardiac biomarker tests helps to detect presence of coronary syndrome and cardiac ischemia to evaluate its severity and diagnosis.
VOLUNTARY PROVISION OF INFORMATION ON BIOMARKERS AND INTERMEDIATEENDPOINTS IN PREVENTION TRIALS NIH GUIDE, Volume 23, Number 12, March 25, 1994 P.T. Keywords: National Cancer Institute The National Cancer Institute (NCI) announces the establishment of a biomarker registry comprised of information that may be useful to investigators when biomarkers are being considered as intermediate endpoints or as outcome measures in prevention trials. In an effort to collect information on biomarkers, the NCI seeks cooperation from the extramural scientific community on a voluntary basis. Information may be submitted by anyone currently engaged in biomarker research. The following characteristics of biomarkers are of interest: 1. Biological Characteristics: The description should include, but is not limited to, the spontaneous biological rate of progression and/or regression, site-specificity, histology, chromosomal aberrations, epigenetic changes, mutations, histologic correlation, and the phase, i.e., ...
Nephrotoxicity is a common side effect of chemotherapy treatment. Traditional blood and urine markers for diagnosis of nephrotoxicity are insensitive and non-specific. This study aimed to investigate the efficiency of kidney injury molecule-1 (KIM-1) as a sensitive biomarker for early kidney injury in chemotherapy treated cancer patients. This study included fifteen female breast cancer patients treated with adrimycin (ADR) and cyclophosphamide (CP). Urinary markers such as KIM-1 and microalbuminuria (MALB) were measured. Glomerular Filtration Rate (GFR), serum levels of creatinine (SCr), blood urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), malonaldehyde (MDA) and electrolytes were analyzed. All markers were detected before and after 24h of treatment. A significant increase in MALB (P , 0.001) and KIM-1 (P , 0.000) levels were observed after chemotherapy treatment while no significant differences in the mean value of GFR, SCr, BUN, and electrolytes levels were found. The ...
The idiopathic nature of UCPPS has prompted an intense search for diagnostic and prognostic clinical biomarkers of this syndrome. The development of clinically...
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YKL-40 is an inflammatory biomarker associated with disease activity and mortality in patients with diseases characterized by inflammation and tissue remodelling. The aim of this study was to describe the prognostic value of YKL-40 in an unselected patient population ...
Identification of Biomarker Panels for Diagnosis of Disease (Jim Osborne, Craig Adams): Biomarkers are measurable entities used to diagnose disease and monitor clinical responses to therapy. Many diseases are difficult to diagnose, and patients with the same diagnosis often respond differently to a given therapy. Most approved diagnostic tests are based on a single biomarker. The Center for Biomarker Research is investigating the use of biomarker panels to better diagnose disease and stratify patient populations for selection of therapy. Flow cytometry is a very powerful technique for measuring multiple markers on the surface and inside of cells. Nucleic acid and protein assays can also be multiplexed in a flow cytometer by designing assays on multicolored beads. Dr. Osborne and The Center for Biomarker Research are developing and validating protocols using multiplex flow cytometry to investigate biomarker panels for diseases that have good therapeutic options, but are difficult to ...
A kind of Structural Testing Method that diagnosis or treatment for chronic are supported and its equipment.Equipment realizes that process is collected in the structuring based on medical service condition and/or problem, structuring collects process and provides at least one or more parameter, and the parameter definition at least one entry criteria, event arrangement, at least one abides by criterion and at least one exits criterion.Entry criteria establishes the condition for needing to meet before obtaining biomarker data from patient.Each event in event arrangement may include implementing time, the guidance for carrying out event, the request to the information from patient, the request to patient motion and at least one of request of collection of biomarker data of at least one type from patient or more.It is used to qualitatively assess in accordance with criterion and solves the problems, such as medical service condition and/or acceptable data according to whether the event that event
TY - JOUR. T1 - The clinical impact of circulating caspase-3 p17 level. T2 - A potential new biomarker for myocardial injury and cardiovascular disease. AU - Singh, Kanwar P.. AU - Jaffe, Allan S. AU - Liang, Bruce T.. PY - 2011/7. Y1 - 2011/7. KW - apoptosis. KW - heart failure. KW - infarct. KW - reperfusion. UR - http://www.scopus.com/inward/record.url?scp=79961076739&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=79961076739&partnerID=8YFLogxK. U2 - 10.2217/fca.11.29. DO - 10.2217/fca.11.29. M3 - Article. C2 - 21797739. AN - SCOPUS:79961076739. VL - 7. SP - 443. EP - 445. JO - Future Cardiology. JF - Future Cardiology. SN - 1479-6678. IS - 4. ER - ...
These four biomarkers were chosen because they provide a characteristic profile that is significant for health-status determination, as said by the researchers. The device can also determine sweat rate and loss, and it can store samples for subsequent laboratory analysis, if necessary.. This device is designed as a one-timer used only for a few hours. In the future, it may be more largely used for disease diagnosis.When a Smartphone is brought into proximity with this device, the wireless electronics activates an app that captures a photo of the device and study the image to yield data on the biomarker concentrations.. During moderate or brisk exercise, sweat winds through the tiny microscopic channels of the device and into the four different small, circular compartments. In the compartments, reactions with chemical reagents result in observable color changes, in ways that quantitatively connect to pH and concentration of glucose, chloride and lactate.. "The aim is for the sensors to come in ...
A key to develop nanodevices with high performance for biosensors applications is to explore advanced functional nanomaterials . Among these materials , 2D - based nanohybrids clearly stand out in the field of electrochemical immunosensors for clinical biomarkers, where they offer unparalleled advan...
The graph, and the table below it, show key results from all meta-analyses. As the overall result, the effect size-a ratio of the mean biomarker concentration of one condition over another (e.g. AD/control)-is plotted against the z score, a statistic indicating how far away the observed ratio is from the null hypothesis of no difference. For example, a z score of 1.96 means that the observed ratio is 1.96 times larger than the standard error; it is equivalent to p=0.05. A z score of 3.89 is equivalent to p=0.0001. Customize the plot by selecting group comparisons and/or biomarkers. The size of each circle reflects the total number of subjects in the meta-analysis. Mouse over the circle to learn more. In the table, click the arrowheads to sort each data column.. ...
Expression of the various markers in normal brain. A-D. CD31, CD34 and collagen IV are all expressed in capillaries. αSMA is variably expressed (panels A, B,
The analysis of extracellular vesicles (EVs) in biofluids is an expanding area of biomarker discovery. To be effective, a diagnostic test must differentiate EVs originating from cancer cells versus non-cancerous origins, and also define biomarkers enriched in cancer EVs. Our team has developed a micro-flow cytometry platform using the Apogee A50 to analyze prostate cancer (PCa) EVs in different biofluids. While flow cytometry is powerful tool for quantitative and qualitative evaluation of cellular biomarkers, direct translation of most applications to EVs is challenging. For example, EVs in biofluids typically range in size from 50-1000nm, and are present as low refractive index, polydisperse populations. We have optimized 30 assay parameters for the identification of prostate specific EVs so that disease specific biomarkers may be evaluated. To illustrate the sensitivity of the system, conditioned media from palmitoylated-GFP-LNCaP cells was used as a positive signal sample. When spiked into ...
At ASCO 2016 I was fascinated to see some of the long-term results of immunotherapy clinical trials which had begun for multipl... | Immuno-oncology | Biomarkers, Emerging science, Immuno-oncology
Children who were later diagnosed with autism spectrum disorder had excessive cerebrospinal fluid and enlarged brains in infancy, a study by a multidisciplinary team of researchers with the UC Davis MIND Institute has found, raising the possibility that those brain anomalies may serve as potential biomarkers for the early identification of the neurodevelopmental disorder. [en español or 中文 Chinese]
Children who were later diagnosed with autism spectrum disorder had excessive cerebrospinal fluid and enlarged brains in infancy, a study by a multidisciplinary team of researchers with the UC Davis MIND Institute has found, raising the possibility that those brain anomalies may serve as potential biomarkers for the early identification of the neurodevelopmental disorder. [en español or 中文 Chinese]
Purpose: There is currently no reliable biomarker to predict who would benefit from anti-PD-1/PD-L1 inhibitors. We comprehensively analyzed the immunogenomic properties in The Cancer Genome Atlas (TCGA) according to the classification of tumor into four groups based on PD-L1 status and tumor-infiltrating lymphocyte recruitment (TIL), a combination that has been suggested to be a theoretically reliable biomarker of anti-PD-1/PD-L1 inhibitors.. Experimental Design: The RNA expression levels of PD-L1 and CD8A in the samples in the pan-cancer database of TCGA (N = 9,677) were analyzed. Based on their median values, the samples were classified into four tumor microenvironment immune types (TMIT). The mutational profiles, PD-L1 amplification, and viral association of the samples were compared according to the four TMITs.. Results: The proportions of TMIT I, defined by high PD-L1 and CD8A expression, were high in lung adenocarcinoma (67.1%) and kidney clear cell carcinoma (64.8%) among solid cancers. ...
Next Professor Mady Hornig from Columbia University, USA, gave a detailed and highly informative account of her work towards developing blood biomarkers. She explained how immunity and gut microbiota can be linked to the brain, its functioning and mood. She talked about the epigenetic changes that can occur in the microbiome during a persons lifetime and the link to serotonin production. She also talked about measuring blood molecules such as cytokines before and after an exercise challenge in ME patients, and comparing these changes to healthy controls. These cytokines being the most promising as potential biomarkers. Although much of what she described cannot be simply summarised, the audience was left with a strong feeling that Professor Hornig is now working determinedly to solve the biochemical riddles produced by ME. ...
To study the question further, the Weill Cornell team recruited 174 people into the trial. They spanned in age from 25 to 86 and each had a reason for being there-either worries about family history or a subjective feeling that their cognition was falling short of where they had been. They completed a comprehensive screening evaluation that included a detailed clinical history, physical examination, anthropometrics, blood biomarkers, APOE genotyping, and cognitive assessments. The trial included people who were completely cognitively normal, those who had a subjective feeling of decline, and others who were cognitively normal but who had positive biomarkers that would point to preclinical AD. These people were placed in the prevention arm of the study. ...
Strategy for identifying putative biomarkers of AD.(A) Experimental scheme for analysis of regulated proteins from post-mortem AD tissues using mass spectrometr
Background: Pioneer studies demonstrate the impact of extreme sport load on the human brain, leading to threatening conditions for athletes health such as cerebral edema. The investigation of brain water diffusivity, allowing the measurement of the intercellular water and the assessment of cerebral edema, can give a great contribution to the investigation of the effects of extreme sports on the brain. We therefore assessed the effect of supra-physiological effort (extreme distance and elevation changes) in mountain ultra-marathons (MUMs) athletes combining for the first time brain magnetic resonance imaging (MRI) and blood parameters.. Methods:This longitudinal study included 19 volunteers (44.2 ± 9.5 years) finishing a MUM (330 km, elevation + 24000 m). Quantitative measurements of brain diffusion-weighted images (DWI) were performed at 3 time-points: Before the race, upon arrival and after 48 h. Multiple blood biomarkers were simultaneously investigated. Data analyses included brain apparent ...
Blood Biomarker Monitoring, Part 1 With the recent rise in athletes switching to a plant-based nutrition pattern, you can find a number of controversial aspect
6. In Trimegas reports on the mother dated 18 June 2013 and 17 July 2013 it said that:. "The CDT screening test has been found to be one of the most accurate blood biomarkers for alcohol abuse because individuals with a daily intake of more than 60 grams of alcohol over more than two weeks have elevated levels of CDT. In regular drinkers their level of CDT continues to be elevated for between two to four weeks after abstaining, depending on the original increase in the level that existed for that individual. That means that for most people who are dependent their elevated CDT level will be detected even if they find themselves able to abstain for a short period before a test is performed.". 7. Trimega, in considering the significance of the raised CDT level as instructed after 25 July 2013, found that it had made a mistake and the CDT figure should have been 0.2% and not 1.6%. Trimega admitted the error and apologised then to the mothers solicitors by email dated 9 August 2013. An interim ...
As you peruse Alzforum news from the AAT-AD/PD conference held last month in Turin, Italy, you may want to catch our three most recent stories on therapeutic approaches, microbial influences, and emerging research tools for Parkinsons disease. Read the whole series, or download a PDF, for the latest on therapeutic tau antibodies, PET imaging, and blood biomarkers for Alzheimers disease, as well as a DIAN-ADNI data comparison and a story on the provocative idea that the incidence of AD is rising, not falling, as has been reported. ...
Placebos are chosen because they have no physiological effects. But, if they dont affect our physiology, how can they trigger responses, let alone responses that reflect our genetic makeup? Yet the lauded 2015 research is beginning to reveal that specific genetic and blood biomarkers correlate well with responsiveness to placebos, even to the extent that a group of genes, dubbed a placebome (a subset of the genome), can be identified as a collective contributor to self-regulation of healing.. While still on pause, lets remember that individuals vary in their response to pharmaceuticals, as we know from personal experience as well as clinical trials. Further, most of these same clinical studies have also found that expectation of treatment can also trigger responses that vary from strongly beneficial to negligible. Informing us that we are receiving a potentially effective drug, but giving us a placebo (an inactive pill that appears identical to the drug) can indeed be effective. Even telling ...
Assays for biomarkers can be very useful in the selection of a lead compound, determining the mechanism of action of a drug, as a surrogate marker for monitoring clinical efficacy, or for identifying intermediate endpoints of success to decrease follow-up time for a specific treatment.
A small number of studies have looked at inflammatory marker response in asthmatics to a physical activity intervention. Serum markers and fractional exhaled nitric oxide (FeNO) being most commonly used. There were a wide range of serum markers assessed including CRP, plasma cytokines, mite-specific IgE, malondialdehyde, glutathione peroxidase (GSH-Px), and superoxide dismutase. Improvement in levels were found in some studies within the groups exercising as well as the groups who had their medication optimised however not all studies showed this. Fractional exhaled nitric oxide (FeNO) when breathed out in high levels is thought to be related to lung inflammation and poorly controlled asthma. The one study assessing FeNO showed a statistically significant decrease in this reading post exercise intervention.. ...
She pointed out that in 1999, nine percent of the population was in their 60s and in 2050, it will increase to 30%. “So onethird of the population is going to be elderly citizens and thus, it’s important to gauge their health condition. Here comes the importance of biomarker (refer to a protein measured in blood whose concentration reflects the severity or presence of
Biomarker Research encompasses every aspect of biomarker investigation from all biomedical disciplines. The journal recognizes that the study of biomarkers ...
Biomarker Research encompasses every aspect of biomarker investigation from all biomedical disciplines. The journal recognizes that the study of biomarkers ...
Distinct genetic signatures can help distinguish responders from nonresponders of chimeric antigen receptor (CAR) T-cell treatment in patients diagnosed with chronic lymphocytic leukemia (CLL).
in Journal of Proteome Research (2011), 10(7), 3160-82. The identification of specific biomarkers obtained directly from human pathological lesions remains a major challenge, because the amount of tissue available is often very limited. We have developed a ... [more ▼]. The identification of specific biomarkers obtained directly from human pathological lesions remains a major challenge, because the amount of tissue available is often very limited. We have developed a novel, comprehensive, and efficient method permitting the identification and absolute quantification of potentially accessible proteins in such precious samples. This protein subclass comprises cell membrane associated and extracellular proteins, which are reachable by systemically deliverable substances and hence especially suitable for diagnosis and targeted therapy applications. To isolate such proteins, we exploited the ability of chemically modified biotin to label ex vivo accessible proteins and the fact that most of these ...
Chih-Ching Wu, Chia-Wei Hsu, Chi-De Chen, Chia-Jung Yu, Kai-Ping Chang, Dar-In Tai, Hao-Ping Liu, Wen-Hui Su, Yu-Sun Chang and Jau-Song Yu ...
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We aimed to characterise where in the brain and when in the course of the disease neuroimaging biomarkers become abnormal. Findings suggesting differential regional and temporal vulnerabilities to Aβ, metabolic decline, and structural atrophy, which should be taken into account when using biomarkers in a clinical setting as well as designing and evaluating clinical trials.
Lab tests can help you improve your future health. Knowing the predictive biomarkers can guide your lifestyle. Predictive biomarkers are tests that ca...
Some children who are unwell can have damage caused to their kidneys either by the illness itself, or the drugs used to treat the illness. Currently we measure a substance called creatinine in blood tests to check how well the kidneys are working. However, creatinine is slow to show any damage to the kidney, so we may not find out that damage has occurred until it is too late to reverse it. Also doing this test means having to take a blood sample. A number of substances, which we call biomarkers, can be measured in the urine. We think that some of these biomarkers might be useful at telling us how well a child s kidneys are working, and whether any damage is occurring ...
We have described what some have called a paradigm shifting pathway of neutrophilic inflammation which, unlike the classic mode associated with...
What is missing in linking overarching determinants like socio-economic status to health and poor ageing is an understanding of the intermediate mechanisms and biological pathways that relate low SES with deterioration of organic parameters. For example, research on immune markers in the Whitehall II study has shown that glucocorticoids and inflammation may in part explain how the body mediates the effects of low SES thus leading to disease, and this is partly independent from common/known risk factors. Also, evidence has accumulated on the key role of epigenetic modifications induced by the experience of psychosocial adversity in initiating physiological dysregulations ...
The aim of this study was to investigate factors such as ß-tubulin class III, GSTP1 and TLE3 to identify possible biomarkers for predicting the efficacy of
Efter centrifugering skall serum/plasma hällas av från blodkropparna. Centrifugerat rör innehållande separationsgel kan förvaras 1 dygn. Serum/plasma kan förvaras 24 h vid 15 - 25°C och 7 dygn vid 2 - 8° C. Fryst vid <-15° C kan serum/plasma förvaras 60 dygn ...
Network biomarker as a new type of biomarkers with protein-protein interactions was initiated and investigated with the integration of knowledge on protein annotations, interaction, and signaling pathway. A number of methodologies and computational programs have been developed to integrate selected proteins into the knowledge-based networks via the combination of genomics, proteomics and bioinformatics. Alterations of network biomarkers can be monitored and evaluated at different stages and time points during the development of diseases, named dynamic network biomarkers. Dynamic network biomarkers should be furthermore correlated with clinical informatics, including patient complaints, history, therapies, clinical symptoms and signs, physicians examinations, biochemical analyses, imaging profiles, pathologies and other measurements.
Scientists in Switzerland have developed a tiny lab on a chip that when implanted just under the skin can track levels of up to five substances in the bl
Currently, autism is diagnosed and treated based on information gathered from clinical assessments and parent questionnaires. ABC-CT will add to this pool of knowledge by developing a more objective battery of tools to measure social function. Using EEG to measure brain function, eye tracking technology to measure visual attention, and automated recording techniques to assess behavior and speech, children aged 6 to 11 will be monitored over a six-month period. In addition to the behavioral measures and biomarker data, this community resource will also include DNA samples from children with ASD and their parents for use in future genetic studies ...
Although most of the literature in reproductive epidemiology focuses on clinically recognized biological events, such as fetal loss or malformations, current and future studies are likely to involve a range of biologic markers, either as outcomes or to indicate exposure or susceptibility. Literature on effect markers such as semen analysis and early pregnancy loss is growing. Advances in molecular
The use of biomarkers to evaluate coagulation status or anticoagulant effect is controversial at best, as there are few reliable biomarkers that are predictive of outcome. Measuring indirect biomarkers, such as drug levels, is ineffective. Measuring anti-Xa and anti-IIa values, or correction of these values by a reversal agent, is a surrogate marker and needs supplementary data to support its direct relevance to the clinical event of interest (i.e., correction of bleeding). , TV Network
Changes in bone biological markers after treatment of Iranian diabetic patients with pioglitazone: No relation to polymorphism of PPAR- γ (Pro12Ala)
E7046 treatment was associated with manageable toxicity in patients with advanced malignancies. No DLTs were reported, and no apparent correlation between TEAE incidence and exposure was observed, indicating that safety did not limit the RP2D selection (within the range of 125 to 750 mg examined in this study).. Pharmacodynamic biomarker analyses showed that treatment with E7046 resulted in significant changes in the circulating gene-expression levels of several EP4-regulated genes including decreased expression of EOMES, PTGER4 (gene encoding the EP4 receptor) and IDO1; and increased expression of CD274 (gene encoding PD-L1) and PDCD1LG2 (gene encoding PD-L2). Additionally, increased expression of the following EP4-regulated cytokines2 7 15-18 was observed: IL-10, IL-8, IL-12p40, IP-10 (CXCL10), CCL5, and CXCL2. These serum biomarker changes indicated that E7046 successfully antagonizes EP4 in the clinical setting and underscores the unique mechanism of action of E7046. Moreover, these results ...
A simple blood test may be able to determine how physically fit you are, according to a new study conducted by scientists at the Stanford University School of Medicine.|br /|
D. Koumoutsea, I. Karydis, P. Thalassinou, V. German, P. Kapralos, S. Papaoikonomou, N. Filiotis, K. Filioti, C. Poziopoulos, I. Koutandos, D. Patsios, K. Lazaridis. Serum fibtinogen levels as potential surrogate marker of cardiovascular disease in oncologic patients. European Journal of Internal Medicine 2011, 22, ...
Ideally, a clearly defined clinical utility should be integrated into the study design at the earliest phase of biomarker discovery that would allow the selection of the right samples and ask the right question to find the right biomarkers. However, the reality of biomarker research is that many biomarkers under study are the results of exploratory studies analyzing expression profiles of clinical samples that are heterogeneous within groups. It is expected that with progression in the stage/phase of the biomarker study and definitely as part of the decision to further develop them into clinical diagnostics, the intended clinical utility of the diagnostics and the corresponding target populations will have to be further refined.. The clinical applications of a biomarker should not be simply defined as biomarker for a particular cancer, or even by terms such as "detection," "screening," or "prognosis" alone. The clinical applications of a biomarker should be defined by their clinical utilities at ...
The addition of three biological markers to conventional breast cancer risk models significantly improved the ability to distinguish women at high and low risk.
Study Objective: To evaluate serum values of cluster of differentiation 95 (CD95/FAS), hypoxia-inducible factor 1-alpha (HIF-1 alpha), and tyrosine kinase receptor 2 (Tie-2) as possible biomarkers of disease presence and ...
Study Objective: To evaluate serum values of cluster of differentiation 95 (CD95/FAS), hypoxia-inducible factor 1-alpha (HIF-1 alpha), and tyrosine kinase receptor 2 (Tie-2) as possible biomarkers of disease presence and ...
HTG Molecular Diagnostics has launched the HTG EdgeSeq Oncology Biomarker Panel for profiling the expression of more than 2,500 oncology-related biomarkers. The panel runs on the fully automated HTG EdgeSeq system, and couples the companys proprietary nuclease protection chemistry with next-generation sequencing. According to the company, the new panel analyzes a comprehensive set of biomarkers for profiling tumor tissues, analyzing cancer pathways, and identifying new biomarkers across both solid tumors and hematolymphoid neoplasms.
Learn how our broad spectrum of immunoassay systems and comprehensive cardiac biomarkers can help manage patients throughout the continuum of cardiovascular disease
IHC refers to the method of identification of tissue antigens by immunological reaction and has become one of the important diagnostic tool in Oncopathology and molecular research. IHC is now widely used for diagnosis of various tumours by identification and localization of specific biomarkers in tumour tissue. Detection of Hormone Receptors like Estrogen and Progesterone in tumour tissue is the gold standard the treatment of breast cancer. IHC identification of specific Receptor proteins like Her2-Neu, C-KIT, EGFR etc. help in Target Therapy in Breast Carcinoma and GIST . Diagnosis and classification of various Leukaemias and Lymphomas are also done by IHC evaluation. IHC Biomarkers play an important role in assessing the prognosis of Lymphomas, especially NHL. Over 200 antibodies are available for tissue diagnosis by IHC. (Cytokeratin, Vimentin, CD117, CD99, CD10, CD30, CD20, PSA, AFP etc.). Tissue antigens are routinely detected by using chromogenic Peroxidase-Anti Peroxidase method in which ...
VXM01 phase I pilot study in patients with operable recurrence of a glioblastoma to examine safety, tolerability, immune and biomarker response to the investigational VEGFR-2 DNA vaccine VXM01 (Study Code VXM01-02-DE) ...
When and how often laboratory tests are done may depend on many factors. The timing of laboratory tests may rely on the results or completion of other tests, procedures, or treatments. Lab tests may be performed immediately in an emergency, or tests may be delayed as a condition is treated or monitored. A test may be suggested or become necessary when certain signs or symptoms appear. Due to changes in the way your body naturally functions through the course of a day, lab tests may need to be performed at a certain time of day. If you have prepared for a test by changing your food or fluid intake, lab tests may be timed in accordance with those changes. Timing of tests may be based on increased and decreased levels of medications, drugs or other substances in the body. The age or gender of the person being tested may affect when and how often a lab test is required. Chronic or progressive conditions may need ongoing monitoring through the use of lab tests. Conditions that worsen and improve may ...
Our overall objective is to develop and standardize methodologies, accelerating the identification and development of non-invasive biomarkers as well as define new strategies to tackle major diseases such as cancer and parasitic diseases. Thus, REDiEX signifies a significant advance towards personalized medicine aiming to develop new tools and markers with high social impact that will generate economic benefits and equity in global health.
Our overall objective is to develop and standardize methodologies, accelerating the identification and development of non-invasive biomarkers as well as define new strategies to tackle major diseases such as cancer and parasitic diseases. Thus, REDiEX signifies a significant advance towards personalized medicine aiming to develop new tools and markers with high social impact that will generate economic benefits and equity in global health.
Tumor heterogeneity is a major hurdle for cancer treatment success. To effectively enroll patients in clinical trials, the identification of predictive biomarkers at the preclinical stage is cruci ...
Title: Non-invasive biomarkers of fetal brain development reflecting prenatal stress: an integrative multi-scale multi-species perspective on data collection and analysis ...
The physiological reserves of elderly patients with cancer were evaluated and the most relevant biomarkers indicating their functional biological age were described in a recent publication by the Elderly Task Force of the European Organization for Research and Treatment of Cancer.
Interview with Razelle Kurzrock, MD, author of Association of Biomarker-Based Treatment Strategies With Response Rates and Progression-Free Survival in Refractory Malignant Neoplasms: A Meta-analysis
There is increasing demand for organ and tissue donations to cater for a growing waiting list of recipients. Serological screening of donors remains the in
Researchers have linked a structural protein called nestin to a particularly deadly form of breast cancer, identifying a new biomarker that could lead to earlier detection and better treatment.