... ,One-step, microplate or cuvet, colorimetric, linear detection range 8 mg/dL to 300 mg/dL. Procedure: 5 min.,biological,biology supply,biology supplies,biology product
... ,One-step, microplate or cuvet, colorimetric, linear detection range 0.2 mg/dL to 20 mg/dL. Procedure: 20 min.,biological,biology supply,biology supplies,biology product
BioAssay Systems Phospolipid Assay Kit (EPLP-100) measures choline-containing phospholipids in biological samples. The absorbance at 570nm or fluorescence intensity at 530/585 nm is proportional to the phospholipid concentration. Detection range: 3 - 200 µM (colorimetric), 0.6 - 20 µM (fluorimetric).
BioAssay Systems fluorometric CellQuanti-Blue Cell Viability Assay Kit (CQBL-05K) is an assay for cell viability, proliferation, and cytotoxcity. This HTS-capable assay involves adding a reagent to the cell culture and measuring the fluorescence after incubation. As low as 100 cells can be quantified.
Cover by D.Meissner WHO/GRA These Guidelines were adopted by the WHO Expert Committee on Biological Standardization at its 59th meeting which took place in Geneva…
Journal Article 2016; 37(Suppl 1): 17-24 PubMed PMID: 28263526 Citation Keywords: Animals, Biological Assay:methods, Environmental Monitoring, Fishes, Toxicity Tests:methods, Waste Water:toxicity, Water Pollutants, Chemical:toxicity,. : Wastewater, especially containing hospital effluents, exhibits high chemical complexity and specificity since it includes various chemicals, biocides, pharmaceuticals, surfactants, radionuclides, disinfectants and pathogens. Biological tests provide true evidence of the wastewater quality and unlike chemical analytical tests show comprehensive pollution effects on the environment and human health. Normalized conventional bioassays are not sensitive enough for ecotoxicological evaluation of wastewater and there is a great need for the development of suitable sensitive bioassays in order to characterize properly the residual toxicity of treated effluents. Provisions of binding EU legislation regarding protection of animals used for scientific purposes and ...
Plasma samples obtained from patients receiving a 24-h continuous infusion of human recombinant (hr)TNF or a combination of two overlapping, 24-h continuous infusions of hrIFN-gamma and hrTNF were analyzed for IL-6 in a sensitive bioassay. A transient appearance of circulating IL-6 was observed with peak levels between 3 and 6 h after the start of the hrTNF infusion. These peak levels correlated quite well with the dose of hrTNF administered (r = 0.86; p less than 0.001). The maximal value observed was 27.5 ng/ml IL-6 in a sample of a patient receiving 545 micrograms/m2 hrTNF. The combination of hrIFN-gamma (200 micrograms/m2) and hrTNF in the infusions resulted in higher IL-6 levels than a comparable dose of hrTNF alone. A maximal value of 23.5 ng/ml IL-6 was observed in a patient receiving 205 micrograms/m2 hrTNF. No IL-6 was found in the plasma of patients during the 12-h infusion with hrIFN-gamma alone, except for two borderline samples. ...
Preclinical CRO for pharmacology, dermatology and cosmetics, in vitro models and bioassays alternative methods to animal testing.
TY - JOUR. T1 - Soluble CD141-152 confers responsiveness to both lipoarabinomannan and lipopolysaccharide in a novel HL-60 cell bioassay. AU - Yu, Weiming. AU - Soprana, Elisa. AU - Cosentino, Giovanna. AU - Volta, Manuela. AU - Lichenstein, Henri S.. AU - Viale, Giovanna. AU - Vercelli, Donata. PY - 1998/10/15. Y1 - 1998/10/15. N2 - CD14 is a pattern recognition receptor involved in the interaction with multiple ligands, including LPS from Gram-negative bacteria and lipoarabinomannan (LAM) from mycobacteria. While the interactions between LPS and soluble CD14 (sCD14) have been analyzed in detail, LAM/CD14 interactions remain uncharacterized due to the lack of suitable functional assays. We describe herein a novel bioassay for the analysis of CD14/ligand interactions. CD14-negative myeloid HL-60 cells up-regulate endogenous CD14 gene expression when stimulated with LPS in the presence of recombinant soluble CD141-348. Using the HL-60 bioassay, we showed that sCD141- 348 confers responsiveness ...
BBI SolutionsBBI solutions為一家領先的開發商以及原料/成品測試製造商,目前已於五個不同的國家設立據點,生產的產品包括:生物原料:抗原、酶、monoclonal / 重組的Fab antibody血清和血漿產品:正常人血清及患者樣本,疾病狀態離子另外還有金奈米粒子及Horseraddish過氧化物及葡萄糖氧化物的生產。BioAssay SystemsBioAssay Systems 公司從事研發和銷售創新高通量的檢測試劑組,以滿足不斷增加需求的生命科學產業。提供的生物測定系統檢測試劑盒,使用簡單方便且性能優越。BioAssay Systems 著重於安全、無放射性、吸收熒光和發光檢測技術,目前的產品包括以下幾個方面:*檢測試劑盒及試劑»血液和尿液化學»代謝/酶活性»陰離子和陽離子»氧化激活»信號轉導»HTS試劑*設備及配件»手持式讀寫器»酶標儀»微孔板*服務»分析服務»含量定制/設計與開發Bioassay Technology LaboratoryBT
C55 High throughput screening technologies have revolutionized the manner in which potential therapeutics are identified. One casualty of the move to high throughput screening technologies has been the widespread disappearance of natural product extracts from pharmaceutical screening programs. Though they are the source of lead compounds for ~65% of anticancer and antimicrobial drugs approved by the FDA between 1981-2002, natural products have often been excluded from modern screening programs. This is due, at least in part, to the inherent difficulties in testing complex extract mixtures, which often contain nuisance compounds, in modern bioassay systems. Here we present a novel electrochemiluminescent assay system that is suitable for testing natural product extracts in high throughput screening systems. To demonstrate its utility, we have developed a screen for inhibition of E3 (ubiquitin ligase) activity utilizing the cancer-relevant molecular target MDM2. Additional screens for inhibition ...
Hi For the last 8 months Ive been working on an in vitro bioassay for FSH. However, I have been struggling with a lack of sensativity when dealing with serum samples which are typically around the 2 ng/ml region. The assay involves incubating sertoli cells from the testes of 7-10 day old rats in the presence of FSH. The presence of FSH then causes a dose-dependent increase in aromatase activity when adrostendione is converted to estradiol which can be measured to determine the concentration of FSH. If anyone out there is familiar with this assay and can help me to increase the sensativity (by decreasing the variability) then please e-mail me Wayne Young Youngwa at agresearch.cri.nz AgResearch New Zealand ...
CHI s Bioassays for Immuno-Oncology Symposium, October 23, 2017, Washington, DC, brings together experts in bioassays for immuno-oncology therapies will discuss selection, development, and standards for bioassays and immunoassays. Special attention will be given to understanding the mechanism-of-action for immunotherapies, whether they be antibody- or cell-based. Overall, this one-day immersive symposium will outline a product life cycle approach for developing and implementing biological assays from preclinical studies to clinical development. This symposium is part of the Immunogenicity & Bioassay Summit.
A method for performing time resolved homogeneous assays using a long-lifetime luminescent dye as a donor. A reaction well containing a sample portion, donor reagent, and acceptor reagent and a matrix well containing a sample portion and donor reagent are excited and the resulting emission from each is measured at a single wavelength associated with the acceptor. The measurement obtained from the matrix well is used to provide a correction for the measurement obtained from the reaction well. The sample may be a biological fluid such as an oral fluid.
In general, all constituents of a multi-component compound are not equally important for the process in hand. If we restrict ourselves to some sort of biological assay or property measure such as logD or pKa, then these are easily understood as reflecting the presence of a parent molecule. Data such as aD reflect the presence of isomeric parent molecule. Note that neither of these parent molecules have an independent existence as a physical entity, in the Daylight sense they are identifiers or names, about which, data exist. Data such as melting point are clearly about a particular sample, as are the experimental results of both physicochemical and biological assays. A version molecule structure may also have been assigned to this sample perhaps a salt or solvate or even a impure isomer.. Calculated data such as cLogP are about the parent molecule, whereas a rubicon structure is a datum about the isomeric parent. An x-ray structure on the other hand is a datum about the sample, and is related to ...
Secondly we were interested in the amount and distribution of PrPSc at 24 mpi, since the extent of this accumulation varied at earlier time points with peaks at 8 and in particular 12 mpi and lows at 16 mpi respectively [10]. However, these earlier studies did also suggest a higher amount in animals at 24 mpi suggesting an undulant pattern of about 12 months. This finding is now supported by the bioassay results, as three out of the four cattle from the 24 mpi group showed levels and distribution of infectivity comparable to the peaks seen at 12 mpi. However it has to be bear in mind that only four animals per time point were investigated here and in earlier studies and that the variations between individuals are very high [10,14]. This is reflected in the present study by variable detection rates in different animals of the 24 mpi group and might explain the differences seen in infectivity levels reported for ileal samples by several authors before [6,9-11,15]. In summary, data presented here ...
Secondly we were interested in the amount and distribution of PrPSc at 24 mpi, since the extent of this accumulation varied at earlier time points with peaks at 8 and in particular 12 mpi and lows at 16 mpi respectively [10]. However, these earlier studies did also suggest a higher amount in animals at 24 mpi suggesting an undulant pattern of about 12 months. This finding is now supported by the bioassay results, as three out of the four cattle from the 24 mpi group showed levels and distribution of infectivity comparable to the peaks seen at 12 mpi. However it has to be bear in mind that only four animals per time point were investigated here and in earlier studies and that the variations between individuals are very high [10,14]. This is reflected in the present study by variable detection rates in different animals of the 24 mpi group and might explain the differences seen in infectivity levels reported for ileal samples by several authors before [6,9-11,15]. In summary, data presented here ...
Enzymes are crucial for a variety of cellular functions (e.g. metabolism, signal transduction). Enzymatic activity assays measure enzyme kinetics and inhibition, by different detection methods (e.g. absorbance, fluorescence, or chemiluminescence).
Clonogenic assays measure the ability of single cells to proliferate and form a colonyThis process approximates closely the regrowth and recurrence of tumors after treatment with radiation or chemotherapyproviding an assay to screen for drugs that block this processYetclonogenic assays are labor intensive and too cumbersome for high throughout screeningHTSof compound librariesHere we propose to de .... ...
PIPETMAX® is an automated pipetting solution for the efficient processing of high-throughput biological assays. It will help you to improve on the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that ...
ZenBio offers a variety of assays to using our ArrayScan XTI. Customers can choose from any of the following assays or we can design a custom program tailored to each clients specific research needs.
Annex 1: Release procedure of International Chemical Reference Substances; Annex 2: WHO good practices for pharmaceutical microbiology laboratories; Annex 3: WHO good manufacturing practices: main principles for pharmaceutical Products; Annex 4: WHO good manufacturing practices for blood establishments (jointly with the Expert Committee on Biological Standardization); Annex 5: WHO guidelines on good manufacturing practices for heating, ventilation and air-conditioning systems for non-sterile pharmaceutical dosage forms; Annex 6: WHO good manufacturing practices for sterile pharmaceutical products; Annex 7: WHO guidelines on transfer of technology in pharmaceutical manufacturing; Annex 8: Good pharmacy practice: standards for quality of pharmacy services (joint FIP/WHO); Annex 9: Model guidance for the storage and transport of time- and temperature-sensitive pharmaceutical products (jointly with the Expert Committee on Biological Standardization); Annex 10: Procedure for prequalification of ...
Looking for online definition of biologic assay in the Medical Dictionary? biologic assay explanation free. What is biologic assay? Meaning of biologic assay medical term. What does biologic assay mean?
Video articles in JoVE about bioassay include Isolating and Using Sections of Bovine Mesenteric Artery and Vein as a Bioassay to Test for Vasoactivity in the Small Intestine, A Simple Bioassay for the Evaluation of Vascular Endothelial Growth Factors, A Fish-feeding Laboratory Bioassay to Assess the Antipredatory Activity of Secondary Metabolites from the Tissues of Marine Organisms, Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles, A Rapid In Vivo Bioassay for Developmentally Active Enhancers, A Small Volume Bioassay to Assess Bacterial/Phytoplankton Co-culture Using WATER-Pulse-Amplitude-Modulated (WATER-PAM) Fluorometry, Human In-Vivo Bioassay for the Tissue-Specific Measurement of Nociceptive and Inflammatory Mediators, Bone Conditioned Medium: Preparation and Bioassay, Isolation and Quantification of Botulinum Neurotoxin From Complex Matrices Using the BoTest Matrix Assays, Screening for Endocrine Activity in Water Using Commercially-available In Vitro
The effects of many chemicals on cellular processes are governed by their ability to enter the cell, which is in turn a function of the composition of the cells external environment. To examine this relationship, the effect of serum in cell culture medium on the bioavailability of cytochrome P450 1A (CYP1A)-inducing compounds was determined in PLHC-1 (Poeciliopsis lucida hepatocellular carcinoma) cells. The presence of 10% calf serum in the medium increased the EC50 (effective concentration to achieve 50% maximal response) for induction of ethoxyresorufin O-deethylase (EROD) activity by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) 20-fold as compared to treatment in serum-free medium. Measurement of [3H]TCDD uptake and Ah receptor binding indicated that the apparent difference in potencies was a result of decreased bioavailability in the presence of serum, effectively reducing the concentration of TCDD within the cells. Induction of EROD and CYP1A protein in response to treatment with each of ...
Host Cell Protein Assay Development. After polyclonal antibody generation, Rockland performs assay development for clients who choose to add that service. Assay development is customized to each individual client. Client can decide to stop the project at the end of multiple project phases. Below is an example of an assay development project for a capture sandwich ELISA that was performed recently by Rockland Immunochemicals.
Several of the pressing scientific challenges in the filed of environmental toxicology are to understand the basic mechanisms of chemically-induced toxic substances and of the design, interpretation, and extrapolation of data from non-human bioassay systems to the human situation. The objective is to offer a new paradigm, namely the idea that cytotoxic and epigenetic effects of chemicals can contribute to many toxic endpoints in human beings. We will speculate that the disruption of calcium homeostasis by a wide variety of chemicals and the conditions of their use might provide an unification of mechanisms leading to either cytotoxicity or epigenetic changes. Depending on the conditions, the effects of this disruption, a pleiotropy of diseases might result, namely, embryotoxicity, teratogenesis, tumor promotion, immune modulation, neurotoxicity, and preproductive dysfunction. In summary, there are at least three biological endpoints at the cell level which chemicals can influence - mutations, cell death
Profiling Animal Toxicants by Automatically Mining Public Bioassay Data: A Big Data Approach for Computational Toxicology Jun Zhang 1,2, Jui-Hua Hsieh 3, Hao Zhu 1,2 * 1 Department of Chemistry, Rutgers
Khan, S. M. M. K., Yoshimura, C., Arikawa, M., Omura, G., Nishiyama, S., Suetomo, Y., Kakuta, S., Suzaki, T. 2006 Axopodial degradation in the heliozoon Raphidiophrys contractilis: a novel bioassay system for detecting heavy metal toxicity in an aquatic environment. Environ. Sci., 13, 193-200 ...
Overall, the company believes there is alignment with the FDA on its panel of analytical methods and the proposed assay qualification/validation plans. Analytical methods are used to assess how reliably and consistently the key product characteristics can be determined in order to ensure patients receive safe and effective product.. In the meeting minutes, the FDA made a request that the company complete implementation of its potency assay qualification plan, presented in the meeting, prior to initiation of upcoming clinical studies. This assay utilizes the Delta 7 mouse model, which has been used historically to assess AVXS-101 potency but now incorporates additional elements to make it acceptable to global regulatory authorities. Specifically, the agency stated, "We believe that your potency assay development and qualification plans have the potential to result in an acceptable potency assay, and we are encouraged that the assay includes reference controls that allow for calculation of ...
Pharmaceutical Information » Question Category » Education & Reference » Pharmacology » Test microorganism used in the microbiological assay of Streptomycin ...
Filtering by: Keyword nucleic acid hybridization Remove constraint Keyword: nucleic acid hybridization Keyword analysis Remove constraint Keyword: analysis Keyword biological assay Remove constraint Keyword: biological assay Rights http://rightsstatements.org/vocab/InC/1.0/ Remove constraint Rights: http://rightsstatements.org/vocab/InC/1.0/ ...
A main presentation track at SLAS 2018 focuses on Assay Development and Screening with emphasis on methods such as high throughput screening, phenotypic screening, and 3rd and 4th dimension screens of cell-based assays.
Course organised by LCBU and gives a practical basis for assay development in high throughput screening and is given as a practical complement to the course
Two industry leading experts have partnered together to take Assay Development to the next level. Lampire Biological Labs and Saladax Biomedical have partnered together to bring you Assay Development, from Concept to Completion. All projects are completed in GMP facilities certified to ISO 13485. We are the only stop you need for your Assay Development. From Pre-Clinical, and Companion Diagnostics, to characterized antibodies and ELISAs, this partnership fills every need you have for your next custom assay. With our expert technical staffs collaborating with you, your next project has to be a success. Dont piece together an assay, get it all at one stop, with our Concept to Completion Assay Development. We look forward to your success!. ...
Hiyoshi began a joint study with XDS in 1998 and came to a licensing agreement in 2000. Since then, Hiyoshi has been working to promote the CALUX Assay in Japan and in Asia. Meanwhile, we have participated in many joint studies with governmental institutes, universities and private companies to prove the superiority of the CALUX Assay ...
Three pressor amines, namely, epinephrine, 3,4-dihydroxyphenethyl-methylamine and 3,4-dihydroxyphenethyldimethylamine were tested for relative potencies by an increasing dose method, and the data were studied to determine when linear relationships might be assumed. The following conclusions may be drawn:. 1. The Weber-Fechner law may be said to hold for restricted ranges of our data but not for the data as a whole.. 2. The application of a progressively increasing dose method to the biological assay of epinephrine may be an improvement upon the U.S.P.XI procedure.. 3. Expressing relative pressor potencies on the basis of doses required to elicit a specific response may not convey a true picture when the characteristic slopes of the logarithmic dose-response curves of two compounds are not identical.. 4. When the logarithmic curves of compounds being compared with epinephrine are dissimilar, it may indicate the presence of qualitative differences in action.. ...
The decimal form should be used when a fraction is given with an abbreviated unit of measure (eg, 0.5 g, 2.7 mm) to reflect the precision of the measurement (eg, 38.0 kg should not be rounded to 38 kg if the scale was accurate to tenths of a kilogram). (See also , Units of Measure, Use of Numerals With Units, Decimal Format.)The patient was receiving gentamicin sulfate, 3.5 mg/kg, every 8 hours. Her serum gentamicin level reached a peak of 5.8 μg/mL and a trough of 0.7 μg/mL after the third dose. Place a zero before the decimal point in numbers less than Less ...
NCDO <- J.O. Mundt, MUTK 6. Plant; USA, Knoxville. Taxonomy/description (3578). Murein: A11.31 (3578). Classification according to the recommendations of the Central Committee on Biological Safety (ZKBS, Germany): Risk group 2. (Medium 92, 37°C ...
animal bioassays could create a lot of controversy with associations such as PETA. a similar association came to my university once and tried to steal cows that were used for digestive processes experiments. even though it might not seem to relevant, i think it is something important to consider when designing an experiment with animals, you never know what these radical associations can make, and ruin years of experiments ...
This course is designed for all individuals who develop, validate, perform or monitor bioassays or immunoassays. Individuals who develop assay methodologies, assign computational and analysis criteria, measure and validate performance characteristics, perform, evaluate and troubleshoot problem assays, or are responsible for the quality of the data will benefit from the lessons and examples presented. Attendees should have a basic understanding of bioassays or immunoassays.
Whether custom conjugation of a ligand to one of our nanoparticles, sample analysis, or full-scale assay development Cytodiagnostics is here to help.Our highly trained technical staff has many years of experience with nanoparticle synthesis, surface modification, analysis, and assay development. This allows us not only
9781574444537 Our cheapest price for Cell-based Bioassays in Drug Discovery And Development is $59.48. Free shipping on all orders over $35.00.
Equipment for collection and concentration of samples from air, surfaces, and liquids for use with rapid microbiological methods ...
Biotherapeutic products (biotherapeutics) have a successful record in treating many life-threatening and chronic diseases. However, their cost has often been high, thereby limiting their access to patients, particularly in developing countries. Recently, the expiration of patents and/or data protection for the first major group of originators biotherapeutics has ushered in an era of products that are designed to be "similar" to a licensed originator product. These products rely, in part, for their licensing on prior information regarding safety and efficacy obtained with the originator products. The clinical experience and established safety profile of the originator products should contribute to the development of similar biotherapeutic products (SBPs). A variety of terms, such as "biosimilar products", "follow-on protein products" and "subsequent-entry biologics" have been coined by different jurisdictions to describe these products.. The term "generic" medicine is used to describe chemical, ...
Joint FAO/WHO Expert Committee on Food Additives is on Rediff pages, ,Follow Joint FAO/WHO Expert Committee on Food Additives to get latest updates from Joint FAO/WHO Expert Committee on Food Additives
CURRENT LUMINESCENCE BIOASSAYS RELY SOLELY UPON MESUREMENT OF LUMINESCENCE INTENSITY TO GENERATE INFORMATION. MULTIPLE COLORS FROM BEETLE LUCIFERASE HAS CREATED THE OPPORTUNITY TO INCREASE ASSAY INFORMATION CONTENT BY USING MULTIPLEXING TECHNIQUES TO MEASURE LIGHT INTENSITIES AT DIFFERENT WAVELENGTHS. THIS PROVIDES GREATER ASSAY RELIABILITY, PRECISION, AND ABILITY TO MEASURE CONCURRENT MOLECULAR EVENTS. THE EXCELLENT PERFORMANCE OF BEETLE LUCIFERASE ENZYMES IN BIOASSAY METHODS YIELDS THE HIGHEST QUANUM EFFICIENCY OF ANY LUMINESCENCE METHODOLOGY, AND DEMONSTRATES SUPERIOR PRECISION, LINEARITY, AND RAPIDITY. TO FURTHER ASSESS THE FEASIBILITY OF USING MULTIPLEXING TO INCRASE THE COMMERCIAL POTENTIAL OF BIOLUMINESCENCE ASSAYS, RESEARCHERS ARE IDENTIFYING NOVEL SUBSTITUTIONS THAT REGULATE COLOR BY EXAMINING THE RELATIONSHIP BETWEEN AMINO ACID SUBSTITUTIONS IN THE PRIMARY STRUCTURES OF BETLE LUCIFERASES TO THE ENZYMES SPECTRAL PROPERTISE ...
en] Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene eXpression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. ...
TY - JOUR. T1 - Bleomycin serum pharmacokinetics as determined by a radioimmunoassay and a microbiologic assay in a patient with compromised renal function. AU - Crooke, Stanley T.. AU - Luft, Friedrich. AU - Broughton, Alan. AU - Strong, James. AU - Casson, Kay. AU - Einhorn, Lawrence. PY - 1977/4. Y1 - 1977/4. N2 - Serum and plasma bleomycin concentrations were determined in a patient with renal dysfunction at two creatinine clearances. The results obtained with a new radioimmunoassay and the microbiologic assay were compared. It was shown: 1) that the clearance of bleomycin from the blood is markedly retarded in severe renal dysfunction, 2) that clearance of bleomycin varies with creatinine clearance, 3) that bleomycin is probably not dialyzable, 4) that determinations on serum and plasma were equivalent, and 5) that the radioimmunoassay and microbiologic assays gave equivalent results (P , 0.001).. AB - Serum and plasma bleomycin concentrations were determined in a patient with renal ...
Background: Today, cells are commonly analyzed in ensembles, i.e. thousands of cells per sample, yielding results on the average response of the cells. However, cellular heterogeneity implies the importance of studying how individual cells respond, one by one, in order to learn more about drug targeting and cellular behavior. Scope of review: This review discusses general aspects on miniaturization of biological assays and in particular summarizes single-cell assays in microwell formats. A range of microwell-based chips are discussed with regard to their well characteristics, cell handling, choice of material etc. along with available detection systems for single-cell studies. History and trends in microsystem technology, various commonly used materials for device fabrication, and conventional methods for single-cell analysis are also discussed, before a closing section with a detailed example from our research in the field. Major conclusions:A range of miniaturized and microwell devices have ...
Topoisomerase II In Vivo Link Kit - quantify the activity of topoisomerase in vivo and determine how a given drug affects its predicted target using the ICE Bioassay technique. Available from TopoGEN.
Để khích lệ tinh thần của những thành viên tâm huyết và những người có bài viết hay, hoặc những bài viết sưu tầm chất lượng, BQT quyết định : -- Những bài viết đó sẽ đc trang trọng đưa lên phần đầu diễn đàn, và đc dán lên cao tại Box có bài viết đó. -- Chủ nhân của những bài viết đó sẽ đc cộng điểm thưởng từ 1-|5 (tùy theo chất lượng bài viết). -- Người tìm và giới thiệu bài viết đó (nếu bài viết đó đc công nhận) sẽ đc cộng 2 điểm. -- Cuối tháng sẽ có đợt tổng kết và người có nhiều bài
The Department never provided the auditors with any information about the Department s current method to assist members or veterans in obtaining information on all available federal treatment services, including, as specifically required by law, a best practice health screening test for exposure to depleted uranium using a bioassay procedure involving sensitive methods capable of detecting depleted uranium at low levels and the use of the equipment with the capacity to discriminate between different radioisotopes in naturally occurring levels of uranium and the characteristic ratio and marker for depleted uranium. When asked about compliance with the Act, the Department merely gave the auditors a memo stating that the task force terminated on January 31, 2008 and the legislation expired before any of the named entities made the appropriate appointments. Further, the Department provided no documentation or communication showing that it attempted to have the appointments to the task force made by ...
ScrubChem: Cleaning of PubChem Bioassay Data. ScrubChem.org is a digital curation of PubChem Bioassay, designed to build datasets and enhance data-driven research. ScrubChem contains approximately 694 million bioactivity values and maps this data to 9 thousand biomolecular targets and 2.3 million chemical structures. ...
Described here are procedures and kits for the selective detection of toxicants in environmental samples. Specifically exemplified are procedures and kits which are used to detect heavy metals. The presence of heavy metals is detected by observing the inhibition by the toxicant of a microbially produced enzyme.
Looking for Bioassays? Find out information about Bioassays. A method for the quantitation of the effects on a biological system by its exposure to a substance, as well as the quantitation of the concentration of a... Explanation of Bioassays
Potency is a critical quality attribute to support development and release of biopharmaceutical products. Researchers assess most protein-drug potencies using biological assays (such as cell-based assays), which mimic a products known mechanism of action or binding assays (if the only known mechanism of action is a drug binding to its target or if a drug is in early phases development). Potency denotes an important feature of complex biologics: their biological activity produced as a direct result of the molecules tertiary/quaternary…. ...
The decimal form should be used when a fraction is given with an abbreviated unit of measure (eg, 0.5 g, 2.7 mm) to reflect the precision of the measurement (eg, 38.0 kg should not be rounded to 38 kg if the scale was accurate to tenths of a kilogram). (See also , Units of Measure, Use of Numerals With Units, Decimal Format.)The patient was receiving gentamicin sulfate, 3.5 mg/kg, every 8 hours. Her serum gentamicin level reached a peak of 5.8 μg/mL and a trough of 0.7 μg/mL after the third dose. Place a zero before the decimal point in numbers less than
A novel and patented platform technology is being developed to create a new method ultimately capable of sequencing over four billion bases a day. The new method will be faster, more accurate and will inaugurate a paradigm shift in the analysis of genomic information. The method is based on the development of digital multibit encoding techniques that utilise advanced magnetic technology. The project has far reaching implications related to the development of advanced systems for ultra high throughput biological assays that can be used in clinical diagnostics, drug discovery and genome sequencing. The generic technology will be capable of tagging billions of compounds synthesised by combinatorial chemistry techniques and decode them in a single experiment. The main advantages of the proposed technology are, the ability to provide remote encoding during the synthesis of chemical or biological moieties, the ability to provide an extremely large number of discrete codes and the ability to rapidly ...
Search Document Centers online database of US and international standards, place orders for documents, learn about the standards industry.
Search Document Centers online database of US and international standards, place orders for documents, learn about the standards industry.
The c-met ELISA includes two highly specific HGFR antibodies. The c-Met HGFR Assay measures human c-Met HGFR in serum, plasma and cell culture
With the drug industrys expanding emphasis on biologics, the need for robust cell-based assays has grown at all stages of development. Requirements for efficacy, quality, and potency testing often demand a complex set of bioassays and/or cell-based assays for new therapeutics or biosimilars. Developers of the latter have found this need for cell-based assays to be particularly challenging. Commercially available, ready-to-use cell-based assays provide a robust functional response from specific therapeutic targets. They can significantly shorten assay development time while…. ...
GxP compliance tools makes the Octet system an ideal analytical instrument in developing validated assay methods for from antibody quantitation, ligand binding to potency assays.
Do you work in product manufacturing for pharmaceuticals, food, or dietary supplements? Are you looking to apply advanced microbiological identification techniques to your industrys manufacturing processes as regulated by the FDA, USDA, and other agencies? Consider this course.
Do you work in product manufacturing for pharmaceuticals, food, or dietary supplements? Are you looking to apply advanced microbiological identification techniques to your industrys manufacturing processes as regulated by the FDA, USDA, and other agencies? Consider this course.
In the four years since the 1978 Symposium on the Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures the use of short-term bioassays to evaluate potential health hazards of complex environmental mixtures has substantially increased. Increased
E2122 - 02(2013) Standard Guide for Conducting In-situ Field Bioassays With Caged Bivalves , bioaccumulation, bivalve, exposure effects, field bioassay, growth, in-situ,
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NordVal is an independent, 3rd party that evaluates the quality characteristics and applications of alternative microbiological methods in the analysis of food, water, feedd and environmental samples. Go to NordVal ...
NordVal is an independent, 3rd party that evaluates the quality characteristics and applications of alternative microbiological methods in the analysis of food, water, feedd and environmental samples. Go to NordVal ...
Testicular function declines with normal aging, while serum immunoreactive LH and FSH levels increase. Since there are reports of an age-related decrease in the ratio of bioactivity to immunoreactivity (B/I ratio) for LH, we used a newly available bioassay for FSH to assess age-associated changes in the bioactivity and B/I ratio of FSH in man. Thirty-nine healthy men (23 young and 16 elderly) had single blood samples drawn. In addition, a subset of these men (12 young and 13 elderly) underwent frequent blood sampling for 24 h, both before and after 7 days of clomiphene citrate (CC) administration. Hourly blood samples from the 24-h sampling were pooled, and these, along with the single samples, were assayed for FSH by an in vitro bioassay system, using estrogen production by immature rat granulosa cells as the end point, and by RIA. Baseline single sample mean FSH, as measured by bioassay, was similar in young and elderly men [386 +/- 98 (+/- SEM) and 342 +/- 77 ng/mL, respectively]. Baseline ...
Citation: Liu, D., Lawrence, M.L., Ainsworth, A.J., Austin, F.W. 2007. Toward an Improved Laboratory Definition of Listeria monocytogenes Virulence. International Journal of Food Microbiology. 118(2):101-15. Interpretive Summary: Listeria monocytogenes is an opportunistic foodborne pathogen that encompasses a diversity of strains with varied virulence. The ability to rapidly determine the pathogenic potential of L. monocytogenes strains is integral to the control and prevention campaign against listeriosis. Early methods for assessing L. monocytogenes virulence include in vivo bioassays and in vitro cell assays. While in vivo bioassays provide a measurement of all virulence determinants of L. monocytogenes, they are not applied routinely due to the requirement for and cost of experimental animals. In vitro cell assays are useful for estimating the virulence of L. monocytogenes strains, but are often slow and sometimes give inconsistent results. Recent identification of novel virulence-specific ...
The synthesis and biological evaluation on thermoregulation of 39 peptides related to bombesin (structural analogues or other naturally occurring peptides) are described. The bioassay system reported measures the ability of peptides injected intracisternally to lower body temperature of cold (4 degrees C) exposed rats. The most potent analogues of bombesin were those in which positions one to five (not included) were altered, indicating that the decapeptide C terminal was sufficient for full potency. Gln at the seventh position and Gly at the 11th position could be replaced by D-Gln and D-Ala (but not D-Pro or D-Phe), respectively, without any change in potency. Methionine at the 14 position could be replaced with its D isomer with retention of 10% biological activity. Any other alteration of the C terminus (deletions or free acid with the exception of the N-methylamide) drastically reduced the biological potency of those peptides. Among other naturally occurring peptides, alytesin was found to ...
Methionine is an essential amino acid for animals and is typically considered one of the first limiting amino acids in animal feed formulations. Methionine deficiency or excess in animal diets can lead to sub-optimal animal performance and increased environmental pollution, which necessitates its accurate quantification and proper dosage in animal rations. Animal bioassays are the current industry standard to quantify methionine bioavailability. However, animal-based assays are not only time consuming, but expensive and are becoming more scrutinized by governmental regulations. In addition, a variety of artifacts can hinder the variability and time efficacy of these assays. Microbiological assays, which are based on a microbial response to external supplementation of a particular nutrient such as methionine, appear to be attractive potential alternatives to the already established standards. They are rapid and inexpensive in vitro assays which are characterized with relatively accurate and consistent
Earthworms have great potential for use as bioassay/biomonitor organisms in studies of contaminant uptake and possess many characteristics that make them ideally suited for this purpose (Ma 1982). Studies have demonstrated that native species of earthworms, collected at contaminated sites, can be used to Indicate biologically available levels of these contaminants (Helmke et al. 1979, Ireland 1983, Pietz et al. 1984). However, it Is the species Eisenia foetida (which does not naturally colonize these sites) which has been recommended for use In the laboratory for the ecotoxicological testing of agricultural and industrial chemicals (European Economic Community (EEC) 1984), proposed as a bioassay species for assessing contaminant availability In waste materials, and used to determine the bioavallability of contaminants In dredged material (Marquenie and Simmers 1984). Correlations between total and OTPA-extractable metal concentrations in contaminated
The assessment of gene expression changes at key developmental periods of the life cycle, as well as at the ages most commonly used in pre-clinical drug safety testing, was the focus of this study. Fischer 344 rats are frequently used in the National Toxicology Program and one of the most commonly used inbred strains for preclinical pharmaceutical in-life testing [25, 33]. The two early time points, 2 and 5 weeks, span the time of weaning (3 weeks). The National Toxicology Program (NTP) 2-year bioassays are typically initiated in 6-8 week old rats [34], thus 6 and 8 week age groups were included to provide baseline for future NTP bioassays. The 15-week age group captures expression data after sexual and physical maturity [35]. Furthermore, a 13-week NTP sub-chronic study, initiated in 8 week old animals would be completed at 21 weeks. Reproductive senescence and aging phenotypes begin to be apparent during the second year [35], thus 52, 78, and 104 week age groups were assessed.. Data analysis ...
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During the last decade, the development of nuclear spin polarization enhanced (hyperpolarized) molecular probes has opened up new opportunities for studying the inner workings of living cells in real time. The hyperpolarized probes are produced ex situ, introduced into biological systems and detected with high sensitivity and contrast against background signals using high resolution NMR spectroscopy. A variety of natural, derivatized and designed hyperpolarized probes has emerged for diverse biological studies including assays of intracellular reaction progression, pathway kinetics, probe uptake and export, pH, redox state, reactive oxygen species, ion concentrations, drug efficacy or oncogenic signaling. These probes are readily used directly under natural conditions in biofluids and are often directly developed and optimized for cellular assays, thus leaving little doubt about their specificity and utility under biologically relevant conditions. Hyperpolarized molecular probes for biological NMR
In forming a view of the burden imposed by regulations, portfolios will be required specifically to assess the extent to which opportunities exist for the acceptance or adoption of trusted international standards and risk assessments, or greater alignment with them, as part of the Governments deregulation agenda. The Government has asked PM&C to track and report progress against this principle, including by publishing progress on repeal days and in annual deregulation reports.. Portfolios are expected to consider the immediate opportunities for applying international standards and risk assessments in the concrete reform priorities that they will develop by the end of 2014 as input into the deregulation forward work programme. Where scope for reform exists for the adoption of international standards and risk assessments, or greater alignment with them, these opportunities will need to be implemented in a manner that is consistent with portfolios delivering on their 2015 and 2016 red tape targets ...
This paper proposes a microbiological method in microtitre plates for the detection of residues of quinolones in milk. The method uses spores of Bacillus licheniformis in culture medium with a redox combination of indicators and gives a response time of 5.5 h. This method detects 92 μg L−1 of ciprofloxacin, 63 μg L−1 of danofloxacin, 109 μg L−1 of enrofloxacin, 101 μg L−1 of marbofloxacin and 109 μg L−1 of sarafloxacin in milk. Therefore, the assay is easy to develop and to use in laboratory, allowing analysis of large numbers of samples at low cost. Due to its good sensitivity to quinolones, this assay can be used as a complementary test of commercial microbiological methods and thereby improve food security ...
0054]A method of pooling as defined herein may be performed by (using) a pooling device. Such a device suitably comprises a sample collector arranged for collecting and delivering a defined amount of sample, for instance in the form of a defined (but variable) volume. A suitable sample collector is a pipettor such as generally applied in robotic sample delivery and processing systems used in laboratories. Such robotics systems are usually bench-top apparatuses, suitably comprising one or more of a microplate processor stages, reagent stations, filter plate aspirators, and robotic pipetting modules based on pneumatics and disposable pipette tips. These sample robot systems are very suitable for performing the method of the present invention as they are ultimately designed to combine different liquid volumes from different samples into one or more reaction tubes. Therefore, it is within the level of skill of the artisan to adapt such a pipetting robotic system to perform the task of combining ...
This article is from Experimental & Molecular Medicine, volume 44.AbstractAptamers are synthetic, relatively short (e.g., 20-80 bases) RNA or ssDNA...
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We recently reported that intraperitoneal administration of leptin-related synthetic peptide 116-130 [LEP-(116-130)] resulted in reduced food intake and significant weight loss in homozygous female C57BL/6J ob/ob mice. In this study, we used two in vitro bioassays to show that the interaction of LEP-(116-130) with the long form of the leptin receptor (OB-Rb), the receptor isoform that is predominantly expressed in the hypothalamus, is not required for the observed in vivo effects of the peptide on energy balance. LEP-(116-130) was unable to compete the binding of alkaline phosphatase-leptin fusion protein to OB-R. Moreover, LEP-(116-130) was unable to activate signal transduction by OB-Rb in vitro. In homozygous female C57BLKS/J-m db/db mice that do not express OB-Rb, intraperitoneal administration of LEP-(116-130) reduced body weight gain and blood glucose levels but not food intake, which further supports a mechanism of action that does not require peptide interaction with OB-Rb. ...
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Antibodies & reagents for reliable immunoassays, ELISA & ELISPOT assays. Quickly design a highly sensitive ELISA or immunoassay. Reagents for veterinary ELISAs.
books.google.ithttps://books.google.it/books/about/Manual_of_microbiological_methods.html?hl=it&id=i9RrAAAAIAAJ&utm_source=gb-gplus-shareManual of microbiological methods ...
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Article Dewatering of moist sediments and soils for application to RaPID Prep AND RaPID assays procedures. BACKGROUND INFORMATION: High moisture content (>30% by weight) can significantly lower concentrations of analytes detected in the RaPID Pre...
Journal of the American Academy of Dermatology - Vol. 80 - N° 6 - p. 1564-1584 - Developing an international standard for the classification of surface anatomic location for use in clinical practice and epidemiologic research - EM|consulte
An improved synthetic, polymer hydrogel permeation layer for use with an active electronic matrix device for biological assays. The permeation layer includes a dried coating of raffinose or a combination of raffinose and stachyose to protect the permeation layer from degradation during shipping and storage.
Photothermal response (PTR) is an established pump and probe technique for real-time sensing of biological assays. Continuous and selective PTR monitoring is difficult owing to the Brownian motion changing the relative position of the target with respect to the beams. Integration of laser trapping with PTR is proposed as a solution. The proposed method is verified on red polystyrene microparticles. PTR is continuously monitored for ...
We want to judiciously use antimicrobial and antimalarials only on the people that really need them. So I think a low cost diagnostic test that you could disseminate more widely would allow us to preserve our antimalarials only for the children who need them which would let them work longer ...
INTEGRA has published a video demonstrating how US-based biotech contract research specialists Likarda LLC are using VIAFLO ASSIST to cost effectively d...
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