The adsorption of benzidine by sepiolite obtained from Eskisehir (Turkey) has been studied by Fourier-transform infrared spectroscopy. A deep green colouration results on reaction of benzidine and sepiolite. In the IR spectrum of benzidine treated sepiolite ν(C-N+) vibrational modes of the uni- and di-positive radical cations are observed in addition to vibrational modes of coordinated benzidine. Spectroscopic results indicate that the principle adsorption mechanism for benzidine is coordination to surface hydroxyls from nitrogen lone pairs and to a relatively small extent, formation of radical cations. No physical sorbed species have been detected. It must be noted that the adsorption of benzidine affected the hydroxyl group vibrations of sepiolite.. ...
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Information about the carcinogenicity of benzidine (92875) in man and in experimental animals is reviewed, along with results of studies on the metabolism of benzidine and azo compounds, and epidemiological surveys of industries that use azo dyes. The author concludes that workers exposed to benzidine based dyes may metabolically convert the dyes to carcinogenic amine. The number of workers with p
The cooxidative metabolism of the urinary bladder carcinogen benzidine was examined using renal inner medullary microsomes. The products of [14C]benzidine metabolism were recovered in the aqueous but not in the organic soluble fraction of reaction mixtures. The reactive metabolites formed during cooxidative metabolism of benzidine bound to DNA and transfer RNA. Cooxidative metabolism of benzidine and subsequent binding to nucleic acids was dependent upon specific fatty acid substrates and was blocked by inhibitors of prostaglandin endoperoxide synthetase. The ratio of the rates of benzidine product formation was approximately 10:3:1 (trichloroacetic acid precipitable:non-trichloroacetic acid precipitable:transfer RNA bound) over a wide range of arachidonic acid concentrations. Cumene hydroperoxide also initiated cooxidative metabolism of benzidine but was less effective than was arachidonic acid. In contrast to arachidonic acid, cumene hydroperoxide-mediated metabolism of benzidine and guaiacol ...
We evaluated the influence of urine pH on the proportion of urinary benzidine (BZ) and N-acetylbenzidine present in the free, unconjugated state and on exfoliated urothelial cell DNA adduct levels in 32 workers exposed to BZ in India. Postworkshift urine pH was inversely correlated with the proportions of BZ (r = -0.78; P , 0.0001) and N-acetylbenzidine (r = -0.67; P , 0.0001) present as free compounds. Furthermore, the average of each subjects pre- and postworkshift urine pH was negatively associated with the predominant urothelial DNA adduct (P = 0.0037, adjusted for urinary BZ and metabolites), which has been shown to cochromatograph with a N-(3-phosphodeoxyguanosin-8-yl)-N-acetylbenzidine adduct standard. Controlling for internal dose, individuals with urine pH , 6 had 10-fold higher DNA adduct levels compared to subjects with urine pH , or = 7. As reported previously, polymorphisms in NAT1, NAT2, and GSTM1 had no impact on DNA adduct levels. This is the first study to demonstrate that ...
The epidemiological studies showed that occupational exposure to commercial benzidine alone was strongly associated with bladder cancer. In the same studies, exposure to 2-naphthylamine alone was similarly associated with bladder cancer. A number of case reports from several countries support the relationship between this neoplasm and occupational exposure to benzidine.. Subsequent evaluations: Vol. 29 (1982); Suppl. 7 (1987). ...
Identification of the N-acetylcysteine conjugate of benzidine formed in the peroxidase activation system.: Benzidine is oxidized by the peroxidase/H2O2 system,
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A dry reagent test strip for determining the concentration of an analyte in a liquid sample is described. The test strip has a matrix containing reagent detection chemistry, which includes an oxidase enzyme that can utilize the analyte as a substrate forming hydrogen peroxide, a benzidine dye precursor, a peroxidase enzyme, and an antipyrine compound. The addition of an antipyrine compound to the reagent detection chemistry provides a standard concentration graph which is substantially linear in a desired range of analyte concentration. The precision and accuracy of reading the test strip are enhanced.
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Microsomal enzyme preparations from dog liver, kidney, and bladder were used to assess the prostaglandin H synthasecatalyzed activation of carcinogenic aromatic amines to bind covalently to proteins and nucleic acids. Benzidine, a urinary bladder carcinogen, bound to protein of bladder transitional epithelial and renal inner and outer medullary microsomes and was dependent upon addition of arachidonic acid, but not upon reduced nicotinamide adenine dinucleotide phosphate. Bladder transitional epithelial microsomes also activated o-dianisidine, 4-aminobiphenyl, and 2-naphthylamine to bind to protein and transfer RNA and benzidine and o-dianisidine to bind DNA. Cosubstrate and inhibitor specificities were consistent with activation by prostaglandin H synthase. Binding of benzidine to protein was not observed with either hepatic or renal cortical microsomes upon addition of arachidonic acid or reduced nicotinamide adenine dinucleotide phosphate.. Prostaglandin H synthase and mixed-function ...
0058] The additional layer 510 may be p-doped. A dopant provided for the additional layer 510 in various working examples is a copper complex. In various working examples, the additional layer 510 is doped with the dopant with a dopant concentration within a range from about 1% to about 20%, for example within a range from about 1% to about 15%, for example within a range from about 2% to about 8%. The following materials may be used as part of the matrix material of the additional layer 110: NPB (N,N-bis(1-naphthyl)-N,N-bis(phenyl)benzidine), β-NPB (N,N-bis(naphthalen-2-yl)-N,N-bis(phenyl)benzidine), TPD (N,N-bis(3-methylphenyl)-N,N-bis(phenyl)benzidine), N,N-bis(1-naphthyl)-N,N-bis(phenyl)-2,2-dimethylbenzidine, spiro-TPD (N,N-bis(3-methylphenyl)-N,N-bis(phenyl)-9,9-spirobifluorene), spiro-NPB (N,N-bis(1-naphthyl)-N,N-bis(phenyl)-9,9-spirobifluorene), DMFL-TPD (N,N-bis(3-methylphenyl)-N,N-bis(phenyl)-9,9-dimethylfluorene, DMFL-NPB ...
Direct Black 19 {H.S. Code}: 3204140000 {Color Index}: C.I. Direct Black 19 {CAS No.}: 6428-31-5 {Molecular Structure}: {Molecular Formula}: C34H27N13Na2
In this work, uniformly-dispersed platinum nanoparticles (PtNPs) were synthesized by a simple chemical reduction method, in which citric acid and sodium borohydride acted as a stabilizer and reducer, respectively. An ultrasensitive colorimetric sensor for the facile and rapid detection of Ag+ ions was constructed based on the peroxidase mimetic activities of the obtained PtNPs, which can catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) by H2O2 to produce colored products. The introduced Ag+ would be reduced to Ag0 by the capped citric acid, and the deposition of Ag0 on the PtNPs surface, can effectively inhibit the peroxidase-mimetic activity of PtNPs. Through measuring the maximum absorption signal of oxidized TMB at 652 nm, ultra-low detection limits (7.8 pM) of Ag+ can be reached. In addition to such high sensitivity, the colorimetric assay also displays excellent selectivity for other ions of interest and shows great potential for the detection of Ag+ in real water samples.
appendicitis.. Principle:. The SeraSpot® Anti-Yersinia-6 IgG / SeraSpot® Anti-Yersinia-6 IgA test is a solid phase immunoassay based on the use of recombinant Yersinia proteins as capture antigens printed in array arrangement (spot array) on the bottom of the wells of 96-well microtiter plates. The recombinant antigens serve as capture molecules for antibodies against Yersinia antigens. Bound antibodies are detected by horseradish peroxidase-(HRP)-labeled antibodies against human antibodies of IgG- or IgA-type by substrate reaction with hydrogen peroxide and 3,3,5,5-tetramethylbenzidine (TMB). At the site of formed immune complexes blue spots are developed by precipitated product from colorless substrate solution. Color intensity is correlated to the antibody concentration. Pale blue to dark blue spots are visible by eye.. Article No.:. ...
Connective tissue diseases are characterized by typical autoantibody profiles, all detectable with SeraSpot® ANA-12 IgG.. Principle of the test:. SeraSpot® ANA-12 IgG is a solid phase immunoassay based on the use of recombinant or purified native proteins / protein complexes and dsDNA as capture antigens printed in array arrangement (microspot array) on the bottom of the wells of 96-well microtiter plates. The antigens serve as capture molecules for autoantibodies to nuclear antigens (ANA). Bound antibodies are detected by Horseradish Peroxidase-(HRP)-labeled antibodies against human antibodies of IgG-type by substrate reaction with hydrogen peroxide and 3,3,5,5-Tetramethylbenzidine (TMB). At the site of formed immune complexes blue spots are developed by precipitated product from colorless substrate solution. Color intensity is correlated to the antibody concentration. Pale blue to dark blue spots are visible by eye.. Art. No.:. ...
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A simple, sensitive and rapid spectrophotometric method for the determination of anions, nitrite and nitrate has been described. Determination of nitrite is by means of reactions involving tetrazotisation of Benzidine, followed by coupling withN,N-dimethylaniline and determination of nitrate is mainly based on reduction of nitrate to nitrite by using granular Zinc in NaCl. The produced nitrite is then availed to tetrazotize the Benzidine, which is then coupled with N,N-dimethylaniline to form pinkish red colored an azo dye in acidic medium. Measured the absorbance of resulted colored dye solution at 538 nm. Amount of reagents required is optimised and the extent of tolerance limit by the diverse ions was investigated. The Beer-Lambert s linearity range for Benzidine- N,N-dimethylaniline couple was found to be 0.05 to 0.3g/mL of nitrite with molar absorptivity 3.475x10Lmol-1cm-1 and sandell s sensitivity 3.3152x10-4gcm-2. The evaluated detection limit and quantization limit for the determination ...
Hydrazobenzene is a hydrazine derivative selected for bioassay by the National Cancer Institute because of the documented carcinogenicity of the parent compound hydrazine and of certain substituted hydrazines. Treatment of hydrazobenzene with hot mineral acid results in the production of benzidine (the so-called "benzidine rearrangement") and hydrazobenzene finds application in the dye manufacturing industry as a precursor of this important dye intermediate and potent carcinogen.. A bioassay of technical-grade hydrazobenzene for possible carcinogenicity was conducted using Fischer 344 rats and B6C3F1 mice. Hydrazobenzene was administered in the feed, at either of two concentrations, to groups of 50 male and 47 to 50 females animals of each species. The time-weighted average dietary concentrations used in the rat bioassay were 0.008, 0.03, 0.004, and 0.01 percent for low dose males, high dose males, low dose females, and high dose females, respectively. The time-weighted average dietary ...
The short-term toxicity to aquatic invertebrates has been evaluated taken into account the already existing data on the Direct Blue 199 Na form (i.e. Direct Blue 199_Na). The usage of information on Direct Blue 199_Na, which has the same main component and with a different counter ion, can be considered as suitable and appropriated because the difference in salification is expected to not influence the characteristics related to the specific end-point. The impurity profile does not impact on the read across proposed. Details on the approach followed are included in the document attached to the IUCLID section 13. The acute toxicity of Direct Blue 199_Na to Daphnia magna was determined according to the OECD Guideline 202. The test was carried out under static exposure conditions for a period of 48 hours, at the following concentrations: 1, 10 and 100 mg/l. After 24 and 48 hours of incubation no significant effects (, 10 % of a total of 40 individuals) were observed at all test concentrations and ...
Megavat Direct Black GV is a black vat dye derived from anthraquinone. It is insoluble in water except during the dyeing process. This product comes in ...
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This study describes the preparation of nanoprotein particles having lignin peroxidase (LiP) using a photosensitive microemulsion polymerization technique. The protein-based nano block polymer was synthesized by cross-linking of ligninase enzyme with ruthenium-based aminoacid monomers. This type polymerization process brought stability in different reaction conditions, reusability and functionality to the protein-based nano block polymer system when compared the traditional methods. After characterization of the prepared LiP copolymer nanoparticles, enzymatic activity studies of the nanoenzymes were carried out using tetramethylbenzidine (TMB) as the substrate. The parameters such as pH, temperature and initial enzyme concentration that affect the activity, were investigated by using prepared nanoLip particles and compared to free LiP. The reusability of the nano-LiP particles was also investigated and the obtained results showed that the nano-LiP particles exhibited admirable potential as a reusable
The MP Diagnostics HIV-1/2 ELISA 4.0 is an antigen sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HIV antigens (gp41, gp120, and gp-36) expressed in E.coli. The conjugate is based on a second set of recombinant HIV antigens expressing the same epitopes as the pre-coated antigens, which is conjugated to horseradish peroxidase. Human serum or plasma, are incubated in these coated wells. HIV-1/2 specific antibodies, if present, will bind to the antigens immobilised on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials and conjugate is added to the wells. The antigens of the conjugate will bind to any antigen-antibody complexes previously formed and excess unbound conjugate are removed by washing. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes ...
This Borrelia burgdorferi (Lyme) assay is based on a synthetic peptide antigen (C6 peptide) in microwell enzyme-linked immunosorbent assay (ELISA) format. The antigen is derived from the vlsE protein of Borrelia burgdorferi. In the assay procedure, diluted cerebrospinal fluid specimens are added and incubated in wells of an antigen-coated microwell plate. Antibodies specific to the C6 peptide in the specimen are bound by the immobilized antigen, and unbound antibodies are removed by wash steps. The bound antibodies are detected by addition of a horseradish peroxidase-conjugated goat antihuman IgG/IgM conjugate. After removal of excess conjugate by further wash steps, a chromogenic peroxidase substrate containing tetramethylbenzidine is added. A blue-green product is produced in wells where antibodies have been bound to the antigen. The color development reaction is quenched by addition of dilute sulfuric acid, after which optical absorbance at 450 nm is measured in each well using an ELISA plate ...
A quantitative method was developed to estimate the concentration of cytochrome (cyt) f in isolated thylakoids, using sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by staining with a heme-specific reagent containing 3,3′,5,5′-tetramethylbenzidine and hydrogen peroxide. This densitometric technique was at least as sensitive as difference spectroscopy. Analysis of thylakoid preparations by densitometry of stained bands using cyt c as standard gave molar ratios of cyt/chlorophyll which were identical to ratios obtained by difference spectroscopy. Densitometric assays demonstrated that the molar ratio of cyt f/chlorophyll decreased during leaf aging in seven higher plants; however, there was a marked difference in the rate at which cyt f was lost from the leaves of different species ...
A dual-cell device has been designed as an oxidase-like mimic with the oxidation of 3,3′,5,5′-tetramethylbenzidine as a model reaction. This dual-cell device could be also used to study oxidase-like nanozymes. It was found that only the catalytic sites for oxygen reduction are essential and necessary for oxidase-li
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Patented July 28, 1936 PATENT OFFICE COLOR-PHOTOGRAPH) BLEACH OUT DYESTUFF LAYERS Bla Gaspar, Brussels, Belgium No Drawing. Application December 29, 1932, Serial No. 649,442. 1932 7 Claims. This invention relates to a method of producing color-photographic bleach-out dyestufi layers. Attempts to produce color photographic images by bleaching processes are very old, and 5 innumerable variations of this process have been proposed. Very appreciable results have been obtained in this direction, but notwithstanding it has not been possible to make the finished images correspondingly durable. In particular 10 there have been used for this purpose practically without exception basic dyestuffs. Reference is certainly contained in the literature to the effect that on occasion substantive or acid dyestuffs have also been investigated for the-pur- 15 pose in question, thus for example by Valenta in the Photographische Rundschau, 1911, page 56 etc.-, but the results obtained were not of a useful kind, ...
Background: Lipopolysaccharide (LPS), a lipoglycan from the outer membrane of Gram-negative bacteria, is an immunomodulatory molecule that stimulates the innate immune response. High levels of LPS cause excessive release of inflammatory mediators and are responsible for the septic shock syndrome. The interaction of LPS with its cognate binding proteins has not, as yet, been structurally elucidated.,br /,,br /,Results: The X-ray crystallographic structure of LPS in complex with the integralouter membrane protein FhuA from Escherichia coli K-12 is reported. It is in accord with data obtained using mass spectroscopy and nuclear magnetic resonance. Most of the important hydrogen-bonding or electrostatic interactions with LPS are provided by eight positively charged residues of FhuA. Residues in a similar three-dimensional arrangement were searched for in all structurally known proteins using a fast template-matching algorithm, and a subset of four residues was identified that is common to known ...
The most widespread reason behind the illness is the lining up of transitional cells within the bladder. Papillary and nonpapillary (or sessile) tumors grow for that reason. Papillary tumors attach to a stalk and have a wart-like appearance. The latter is far less common but is more obtrusive and has a more extreme outcome. While the exact cause is still doubtful, growth may be due to one or more of the following.. Smoking - Researchers suggest there is a good link between the illness and tobacco use. Nearly half the cases in American males, and a growing number in women, have a relation to cigarette smoke.. Chemical Exposure - Some workplaces may also put you at higher risk for developing the disease. Nearly thirty percent of cases involve people whose occupations regularly expose them to harmful carcinogens like Benzidine and Napthtylamine. Relevant to this cause are concerns dealing with the use of Actos.. Actos bladder ...
본 연구는 학습과 기억에서 중요한 역할을 담당하는 해마형성체(hippocampal formation)와 내후각뇌피질(entorhinal cortex)에서 노화에 따른 세포사 관련물질들의 변화를 비교분석하여, 노화에 따른 중추신경계의 기능적 변화를 유발하는 기전을 추정하는데 필요한 형태학적 기초 자료를 제공하고자 시행되었다. 연구방법은 노화쥐와 대조군에 4% paraformaldehyde를 심장을 통해 관류하여 고정시키고, 뇌를 적출한 후 40μm 두께로 냉동절단하여 면역세포화학 염색을 하였다. NOS, VIP, c-fos bcl-2, bax, p53에 대한 항체를 각각 희석하여 사용하였고 avidin-biotin peroxidase 용액에서 반응시켰다. 그 다음에 peroxidase를 포함한 3,3-diamino benzidine 용액에서 발색시키고 gelatin-coated slide에서 말린 후 광학현미경하에서 관찰하였다. NOS와 bcl-2의 경우는 면역세포화학 염색이 선명하지 않아 in situ ...
United tates Patent 3,232,755 PHOTOCONDUCTIVE LAYERS FOR ELECTRG- PHOTUGRAPHEC PURPOSES Helmut I-ioegl, Wieshaden, Erwin Lind, Wieshaden- Biebrich, and Heinz Schlesinger, lViesbaden, Germany, assignors, by mesne assignments, to Azoplate Corporafion, Murray Hill, N1, a corporation of New Jersey No Drawing. Filed lune 13, 1960, Ser. No. 35,493 Claims priority, application Germany, July 1, 1959, K 38,111 32 Claims. (Cl. 96- 1) Inorganic materials, such as selenium or zinc oxide, as Well as various organic low molecular weight substances, such as anthracene, chrysene, and benzidine, have previously been used for electrophotographie purposes. Photoconductive layers have now been found which are especially suited for electrophotographic usages and which consist, at least partially, of one or more polymerization products or copolymers of heterocycles having vinyl substituents, and/or substitution products thereof. Photoconductive substances suitable for use in the present invention are polymers and ...
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Benzidine, N,N,N,N-tetramethyl-; 4,4-Bis(N,N-dimethylamino)biphenyl; N,N,N,N-Tetramethyl-p,p-benzidine; [1,1-Biphenyl]-4,4-diamine,N,N,N,N-tetramethyl ...
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INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) C. I. DIRECT BLUE 218 NTP Experiment-Test: 05130-03 Report: PEIRPT03 Study Type: CHRONIC Date: 09/17/94 Route: DOSED FEED Time: 01:29:33 Facility: Microbiological Associates Chemical CAS #: 10401-500 Lock Date: 11/21/91 Cage Range: All Reasons For Removal: All Removal Date Range: All Treatment Groups: Include All a Number of animals examined microscopically at site and number of animals with lesion Page 1 NTP Experiment-Test: 05130-03 INCIDENCE RATES OF NONNEOPLASTIC LESIONS BY ANATOMIC SITE (a) Report: PEIRPT03 Study Type: CHRONIC C. I. DIRECT BLUE 218 Date: 09/17/94 Route: DOSED FEED Time: 01:29:33 ____________________________________________________________________________________________________________________________________ FISCHER 344 RATS FEMALE 0 PPM 1000 PPM 3000 PPM 10000PPM ____________________________________________________________________________________________________________________________________ DISPOSITION ...
Sigma-Aldrich offers abstracts and full-text articles by [Amit Bafana, Sivanesan Saravana Devi, Kannan Krishnamurthi, Tapan Chakrabarti].
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A. Wiebe, B. Riehl, S. Lips, R. Franke, S. R. Waldvogel, Unexpected high robustness of electrochemical cross-coupling for a broad range of current density, Science Advances, 2017, DOI 10.1126/sciadv.aao3920.. M. Hu, K. Korschelt, P. Daniel, K. Landfester, W. Tremel, M. B. Bannwarth, Fibrous nanozyme dressings with catalase-like activity for H2O2 reduction to promote wound healing, ACS Applied Materials & Interfaces, 2017, DOI 10.1021/acsami.7b12212.. M. Shiraiwa, Y. Li, A. P. Tsimpidi, V. A. Karydis, T. Berkemeier, S. N. Pandis, J. Lelieveld, T. Koop, U. Pöschl, "Global distribution of particle phase state in atmospheric secondary organic aerosols", Nature Communications, 2017, 8, Article Number: 15002.. B. Conings, S. A. Bretschneider, A. Babayigit, N. Gauquelin, I. Cardinaletti, J. Manca, J. Verbeeck, H. J. Snaith, H. G. Boyen, " Structure-Property Relations of Methylamine Vapor Treated Hybrid Perovskite CH3NH3PbI3 Films and Solar Cells", ACS Applied Materials & Interfaces, 2017, 9(9), ...
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Wavelength range: 190 to 1,100 nm; 4 nm spectral bandpass. Determination of the color of water and solutions, detection of the presence of dyestuff, quantification of dissolved ions and compounds, etc ...
A photo-sensitive composition for dry formation of image comprising a dyestuff having a photo-fading property, a ketonic sensitizing agent and a high molecular polymer as a binding agent, which can provide a visible image by the irradiation of light and maintain the image without any fixing treatment.
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