α-L-Arabinofuranosidase B25 (Bacteroides ovatus) [ME-ABFBO25] - High purity α-L-arabinofuranosidase (Bacteroides ovatus) for use in research, biochemical enzyme assays and in vitrodiagnostic analysis. EC 3.2.1.55 CAZy Family: GH43 CAS: 9067-74-7 non-reducing end alpha-L-arabinofuranosidase; alpha-L-arabinofuranoside non-reducing end alpha-L-arabinofuranosidase Recombinant. From Bacteroides ovatus. In 50% Glycerol. Specific activity: ~ 25 U/mg protein (on wheat arabinoxylan) at pH 6.5 and 40oC. Stability: | 4 years at -20oC.
It has been known for decades that many human gut bacteria have the ability to utilize a wide variety of plant polysaccharides (PS) and animal glycans for their metabolism and growth (1, 2). The members of Bacteroides, a genus with numerous species abundant in the human colon, are especially adept at utilizing the vast array of PS and glycans that arrive to the colon from the diet or are present on the mucins overlying the epithelial layer. Early studies analyzing genes necessary for starch utilization in Bacteroides thetaiotaomicron led to the identification of the sus locus (3-6), the first characterized locus of a Bacteroides species encoding a cluster of genes necessary to utilize a complex carbohydrate. It was not until the publication of the first Bacteroides genome sequence in 2003 (7) that the vast number of these polysaccharide utilization loci (PUL), as they were named (8), was appreciated. The genome of the B. thetaiotaomicron type strain contains 88 PUL with an average of 10 genes ...
Rutin and quercitrin are hydrolysed to quercetin, and robinin is hydrolysed to kaempferol, by faecal flora from healthy subjects. The enzymes required for these hydrolyses, namely alpha-rhamnosidase and beta-galactosidase, were produced by some strains of Bacteroides distasonis; other strains, however, synthesized beta-glucosidase. The last-named enzyme was also elaborated by Bacteroides uniformis and Bacteroides ovatus. All the enzymes were produced constitutively. A cell-free extract of B. distasonis containing beta-glucosidase displayed an enzymic activity of 1 mumol/10 min per 10 mg of protein. ...
Bacteroides spp. are dominant components of the phylum Bacteroidetes in the gut microbiota and prosper in glycan enriched environments. However, knowledge of the machinery of specific species isolated from humans (like Bacteroides uniformis) contributing to the utilization of dietary and endogenous sources of glycans and their byproducts is limited. We have used the cutting-edge nanopore-based technology to sequence the genome of B. uniformis CECT 7771, a human symbiont with a proven pre-clinical efficacy on metabolic and immune dysfunctions in obesity animal models. We have also used massive sequencing approaches to distinguish the genome expression patterns in response to carbon sources of different complexity during growth. At genome-wide level, our analyses globally demonstrate that B. uniformis strains exhibit an expanded glycolytic capability when compared with other Bacteroides species. Moreover, by studying the growth and whole-genome expression of B. uniformis CECT 7771 in response to different
TY - JOUR. T1 - An anaerobic bacterium, Bacteroides thetaiotaomicron, uses a consortium of enzymes to scavenge hydrogen peroxide. AU - Mishra, Surabhi. AU - Imlay, James A.. PY - 2013/12. Y1 - 2013/12. N2 - Summary: Obligate anaerobes are periodically exposed to oxygen, and it has been conjectured that on such occasions their low-potential biochemistry will predispose them to rapid ROS formation. We sought to identify scavenging enzymes that might protect the anaerobe Bacteroides thetaiotaomicron from the H2O2 that would be formed. Genetic analysis of eight candidate enzymes revealed that four of these scavenge H2O2in vivo: rubrerythrins 1 and 2, AhpCF, and catalase E. The rubrerythrins served as key peroxidases under anoxic conditions. However, they quickly lost activity upon aeration, and AhpCF and catalase were induced to compensate. The AhpCF is an NADH peroxidase that effectively degraded low micromolar levels of H2O2, while the catalytic cycle of catalase enabled it to quickly degrade ...
Define Bacteroides salivosus. Bacteroides salivosus synonyms, Bacteroides salivosus pronunciation, Bacteroides salivosus translation, English dictionary definition of Bacteroides salivosus. Noun 1. Bacteroides - type genus of Bacteroidaceae; genus of Gram-negative rodlike anaerobic bacteria producing no endospores and no pigment and living in...
S. Hsieh designed the project, performed experiments, and wrote the paper. N.T.P. generated the single CPS-expressing B. thetaiotaomicron mutants with and without GFP. D.L.D. generated the B. thetaiotaomicron Abs. S. Horvath processed the fecal colonization samples. G.S. performed the quick-freeze, deep-etch scanning electron microscopy. N.Z. assisted with peritoneal experiments. B.T.S. and B.Z. captured the india ink images. E.C.M., T.S.S., and P.M.A. guided the overall project design and assisted in data interpretation and writing of the manuscript. ...
Probiotic yogurts and pills promise better health, but the bacteria we ingest find a complex and challenging environment when they enter our gut. Food sources shift with every meal we eat, and the threat of pathogens is ever-present. In this competitive landscape, theres a clear winner already on the field: the genus Bacteroides makes up 30 percent of the bacteria in the human gut, the single most abundant genus.. Bacteroides are a successful bacterium in our gut microbiome. By offering metabolic capabilities that humans dont have, it helps us to obtain important nutrients. So why are Bacteroides so resilient? If we can understand their success, it will ultimately allow us to design probiotic formulations for human health and aid patients with an imbalance of gut bacteria, said Blanca Barquera, an associate professor of biological sciences at Rensselaer Polytechnic Institute and lead researcher on a new project investigating Bacteroides.. With support from a four-year $3 million grant from ...
382 aa; Sequence (Fasta) Identical sequences: Bacteroides vulgatus PC510: D4VC18; Bacteroides sp. 3_1_40A: E5UVJ4; Bacteroides sp. 4_3_47FAA: C6Z9F1; Bacteroides vulgatus: A6KX05; Bacteroides vulgatus str. 3975 RP4: A0A069SLD6 ...
Bacteroides coprosuis Whitehead et al. 2005 belongs to the genus Bacteroides, which is a member of the family Bacteroidaceae. Members of the genus Bacteroides in general are known as beneficial protectors of animal guts against pathogenic microorganisms, and as contributors to the degradation of complex molecules such as polysaccharides. B. coprosuis itself was isolated from a manure storage pit of a swine facility, but has not yet been found in an animal host. The species is of interest solely because of its isolated phylogenetic location. The genome of B. coprosuis is already the 5th sequenced type strain genome from the genus Bacteroides. The 2,991,798 bp long genome with its 2,461 protein-coding and 78 RNA genes and is a part of the Genomic Encyclopedia of Bacteria and Archaea project.
Bacteroides ovatus ATCC ® 8483™ Designation: TypeStrain=True Application: Quality control strain for API and BBL products. Control strain for anaerobe identification.
Bacteroides ovatus ATCC ® 8483™ Designation: TypeStrain=True Application: Quality control strain for API and BBL products. Control strain for anaerobe identification.
Bacteroides is a genus of Gram-negative, rod-shaped bacteria. Bacteroides species are non-endospore-forming, anaerobes, and may either be motile or non-motile, depending on the species.[1] The DNA base composition is 40-48% GC. Unusual in bacterial organisms, Bacteroides membranes contain sphingolipids. They also contain meso-diaminopimelic acid in their peptidoglycan layer. Bacteroides are normally commensal, making up the most substantial portion of the mammalian gastrointestinal flora,[2] where they play a fundamental role in processing of complex molecules to simpler ones in the host intestine. As many as 1010-1011 cells per gram of human feces have been reported.[3] They can use simple sugars when available, but the main source of energy is polysaccharides from plant sources. ...
A molecular method, termed hierarchical oligonucleotide primer extension (HOPE), was used to determine the relative abundances of predominant Bacteroides spp. present in fecal microbiota and wastewaters. For this analysis, genomic DNA in feces of healthy human adults, bovines, and swine and in wastewaters was extracted and total bacterial 16S rRNA genes were PCR amplified and used as the DNA templates for HOPE. Nineteen oligonucleotide primers were designed to detect 14 Bacteroides spp. at different hierarchical levels (domain, order, cluster, and species) and were arranged into and used in six multiplex HOPE reaction mixtures. Results showed that species like B. vulgatus, B. thetaiotaomicron, B. caccae, B. uniformis, B. fragilis, B. eggerthii, and B. massiliensis could be individually detected in human feces at abundances corresponding to as little as 0.1% of PCR-amplified 16S rRNA genes. Minor species like B. pyogenes, B. salyersiae, and B. nordii were detected only collectively using a primer ...
Chondroitinase ABC is an enzyme from Bacteroides thetaiotaomicron that cleaves the endo-1,4-β-galactosaminic bonds between acetylgalactosamine and either glucuronic or i
In Ian Lipkin et al 2017 study, low bacteroides caccae was reported (on average). [Index to all posts on Study]. the elevated biotin level was due to an alteration in metabolism related to biotin synthesis by Bacteroides caccae in this mouse model. So low Vitamin B7 Fermentation of pectin and glucose, and activity of pectin-degrading enzymes in…
Accepted name: α-mannan endo-1,2-α-mannanase. Reaction: Hydrolysis of the terminal α-D-mannosyl-(1→3)-α-D-mannose disaccharide from α-D-mannosyl-(1→3)-α-D-mannosyl-(1→2)-α-D-mannosyl-(1→2)-α-D-mannosyl side chains in fungal cell wall α-mannans.. Systematic name: α-mannan glucosylmannohydrolase. Comments: The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-α-1,2-mannosidase.. Links to other databases: BRENDA, EXPASY, KEGG, MetaCyc, CAS registry number: References:. 1. Hakki, Z., Thompson, A.J., Bellmaine, S., Speciale, G., Davies, G.J. and Williams, S.J. Structural and kinetic dissection of the endo-α-1,2-mannanase activity of bacterial GH99 glycoside hydrolases from Bacteroides spp. Chemistry 21 (2015) 1966-1977. [PMID: 25487964]. 2. Cuskin, F., Lowe, E.C., Temple, M.J., Zhu, Y., Cameron, E.A., Pudlo, N.A., Porter, N.T., Urs, K., Thompson, A.J., Cartmell, ...
Cooperative phenotypes are considered central to the functioning of microbial communities in many contexts, including communication via quorum sensing, biofilm formation, antibiotic resistance, and pathogenesis. The human intestine houses a dense and diverse microbial community critical to health, yet we know little about cooperation within this important ecosystem. Here we test experimentally for evolved cooperation within the Bacteroidales, the dominant Gram-negative bacteria of the human intestine. We show that during growth on certain dietary polysaccharides, the model member Bacteroides thetaiotaomicron exhibits only limited cooperation. Although this organism digests these polysaccharides extracellularly, mutants lacking this ability are outcompeted. In contrast, we discovered a dedicated cross-feeding enzyme system in the prominent gut symbiont Bacteroides ovatus, which digests polysaccharide at a cost to itself but at a benefit to another species. Using in vitro systems and gnotobiotic mouse
Bacteroides barnesiae has a size of 0.5-1.4 micrometer wide and 0.8-10.6 micrometer long that is completely anaerobic and grows at temperature of about 37C which does not require oxygen to survive. It has a cellular fatty acids including C15:0 and iso-C15:0 and in cultured media appears to be circular, raised and grayish-white colonies. Bacteroides barnesiae is predominantly found in gastrointestinal tract of humans as well as in vertebrate animals where bile is present. It gives benefits to the host if this bacterium strictly retained in the gut by hindering pathogenic microorganisms from forming colonies in the intestines. Bacteroides barnesiae play a basic role in the breakdown of polysaccharides a complex molecule into small molecules that is used by the bacterium as well as the host in order to utilize the nitrogenous substances and biotransformation of steroids and bile acids. Scientific classification: ...
Dernières PublicationsDietary pectic glycans are degraded by coordinated enzyme pathways in human colonic Bacteroides. (2018) Luis AS, Briggs J, (...)
Bacteroides barnesiae Lan et al. 2006 is a species of the genus Bacteroides, which belongs to the family Bacteroidaceae. Strain BL2T is of interest because it was isolated from the gut of a chicken and the growing awareness that the anaerobic microbiota of the caecum is of benefit for the host and may impact poultry farming. The 3,621,509 bp long genome with its 3,059 protein-coding and 97 RNA genes is a part of the Genomic Encyclopedia of Type Strains, Phase I: the one thousand microbial genomes (KMG) project.
Because conventional microflora are composed of diverse microbial societies, we asked whether single enteric bacterial species are sufficient to drive small intestinal RegIIIγ expression. As expected, a mixed microbial community recovered from a conventional mouse elicited a ∼20-fold increase in RegIIIγ expression when introduced into germ-free wild-type C57/b6 mice. In contrast, colonization with the Gram-negative symbiont Bacteroides thetaiotaomicron elicited only a 2.5-fold increase in expression, whereas the noninvasive Gram-positive L. innocua had no effect on RegIIIγ mRNA levels (Fig. 4C). These results indicate that neither organism alone was sufficient to stimulate RegIIIγ expression to conventional levels in wild-type mice. However, bacteria that are normally strictly compartmentalized in the intestinal lumen show increased mucosal adherence and invasion in mice that lack mucosal IgA (23). We therefore postulated that mucosal defenses such as secretory IgA might be sufficient to ...
Alpha-galactosidase is a glycoside hydrolase enzyme that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. It is encoded by the GLA ge
the organism lacks the pathway. GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page). For more information, see the paper from 2019 on GapMind for amino acid biosynthesis, or view the source code.. If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know. by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory. ...
After several months the gut microbiomes between the two set of infants became more similar with one striking difference - the c-section babies had significantly lower levels of Bacteroides, a strain vital to human health.. Bacteroides are a key strain when it comes to health challenges modern societies face. A number of studies have shown Bacteroides levels are lower in people with obesity. Studies in both mice and humans show that when gut bacteria from thin subjects are transplanted into the colons of obese subjects, most subjects lose weight.. Bacteroides has also been linked with preventing anxiety, and boosting and regulating immunity to prevent inflammatory disorders. This may explain why people who were born via c-section are at increased risk for obesity and asthma.. The study is part of a larger Baby Biome study that is following thousands of newborns through childhood. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
MIT researchers develop basic computing elements for bacteria by encoding a series of sensors, memory switches, and circuits into the common human gut bacterium Bacteroides thetaiotaomicron.
Glycoside hydrolases of family GH76 are endo-acting α-mannanases. GH76 genes are found within bacteria and fungi. Bacterial GH76 enzymes cleave α-1,6-mannans, such as those found within the α-1,6-linked backbone of fungal mannoproteins and mycobacterial cell wall lipomannan and lipoarabinomannan. This family was originally created from the cloning and characterization of Aman6 from Bacillus circulans TN-31 [1], which appears to be the same enzyme as that characterized much earlier by Ballou and co-workers [2]. Aman6 degrades α-1,6-mannan to a mixture of the mannobiose and mannotriose [1]; mannotriose is the minimum substrate for the enzyme [2]. A possible GH76 enzyme has been detected within Mycobacterium smegmatis, which has the capacity to degrade α-1,6-mannooligosaccharides [3]. Additional characterized GH76 enzymes include several from Bacteroides thetaiotaomicron [4]. B. thetaiotaomicron expresses numerous GH76 enzymes. Several of these are found within polysaccharide utilization loci ...
Forty women supplied fecal samples for profiling, and magnetic resonance images were taken of their brains as they viewed images of individuals, activities or things that evoked emotional responses. The women were divided by their gut bacteria composition into two groups: 33 had more of a bacterium called Bacteroides; the remaining seven had more of the Prevotella bacteria. The Bacteroides group showed a greater thickness of the gray matter in the frontal cortex and insula, brain regions involved with the complex processing of information. They also had larger volumes of the hippocampus, a region involved in memory processing. The Prevotella group, by contrast, showed more connections between emotional, attentional and sensory brain regions and lower brain volumes in several regions, such as the hippocampus. This groups hippocampus was less active while the women were viewing negative images. They also rated higher levels of negative feelings such as anxiety, distress, and irritability after ...
Bacteroides, Bacterial Identification and Bacteria Treatment are considered an interesting alternative to the traditional indicator organisms such as E.coli
Bacteroides ovatus is a member of the human gut microbiota. The importance of this microbial consortium involves the degradation of complex dietary glycans mainly conferred by glycoside hydrolases. In this study we focus on one such catabolic glycoside hydrolase from B. ovatus. The enzyme, termed BoMan26A, is a -mannanase that takes part in the hydrolytic degradation of galactomannans. The crystal structure of BoMan26A has previously been determined to reveal a TIM-barrel like fold, but the relation between the protein structure and the mode of substrate processing has not yet been studied. Here we report residue-specific assignments for 95% of the 344 backbone amides of BoMan26A. The assignments form the basis for future studies of the relationship between substrate interactions and protein dynamics. In particular, the potential role of loops adjacent to glycan binding sites is of interest for such studies. ...
Intestinal worms continue to be bad news in most parts of the world, but theyve cohabited with humans for so long that they have evolved to interact with us in complex, even beneficial ways, as a new study published in Science on April 14 reminds us. Led by Deepshika Ramanan from New York University, a team of scientists from the US, Malaysia and Japan worked with mice which had a gene called Nod2 knocked out. Nod2-deficient mice are used as models of inflammatory bowel disease (IBD), a condition that is notably uncommon in developing nations but increasingly prevalent in developed nations.. The scientists found that a type of intestinal cell in the sick mice was defective and resulting in compromised mucus layers lining their guts. These changes in the guts environment caused normal bacterial populations in the gut to fluctuate. One species, Bacteroides vulgatus, was observed to thrive in these IBD mice. Interestingly, B. vulgatus has previously been found in humans with IBD. However, it is ...
General Information: This is the type strain for this organism and was isolated from the feces of a healthy adult. Common gastrointestinal bacterium. This group of microbes constitute the most abundant members of the intestinal microflora of mammals. Typically they are symbionts, but they can become opportunistic pathogens in the peritoneal (intra-abdominal) cavity. Breakdown of complex plant polysaccharides such as cellulose and hemicellulose and host-derived polysaccharides such as mucopolysaccharides is aided by the many enzymes these organisms produce. Bacteroides thetaiotaomicron is one of the two major Bacteroidesspecies found in the intestine. This organism has been used in studies on gut microflora composition and succession. ...
4HBR: Crystal structure of a putative periplasmic proteins (BACEGG_01429) from Bacteroides eggerthii DSM 20697 at 2.40 A resolution
[좌측부터 체질 1형 2형 3형] 1) 박테로이데스(Bacteroides) : 탄수화물을 분해하고 비타민 B2, B5, C, H를 만드는 것이 주된 기능이다. 박테리오데스 세균이 많은 속에 속하는 피실험자들 중에는 비만을 겪는 사...
While more hospitals say they meet stage 1 of the electronic health record incentive program, 53% say they still arent ready, finds study.
This investigation examined the ability of cells of Bacteroides melaninogenicus subsp. asaccharolyticus 381 to adhere to surfaces that might be important for its initial colonization of the mouth and its subsequent colonization in periodontal pockets. Of 48 asaccharolytic strains of B. melaninogenicus, 47 agglutinated human erythrocytes, whereas none of 20 fermentative strains, which included reference cultures of the subspecies intermedius and melaninogenicus, were active. Electron microscopy indicated that both asaccharolytic and fermentative strains possessed pili; hence, the presence of pili did not correlate with the hemagglutinating activities of B. melaninogenicus strains. Both asaccharolytic and fermentative B. melaninogenicus strains suspended in phosphate-buffered saline adhered in high numbers to buccal epithelial cells and to the surfaces of several gram-positive bacteria tested, including Actinomyces viscosus, A. naeslundii, A. israelii, Streptococcus sanguis, and S. mitis. B. ...
Various subspecies of Bacteroides melaninogenicus differ in their pigmentation. Subsp. asaccharolyticus produces protohaem almost exclusively, subsp. intermedicus both protohaem and a smaller proportion of protoporphyrin, and subsp. melaninogenicus mainly protoporphyrin with a trace of protohaem. As a consequence young colonies can be differentiated by their red fluorescence in u.v. light (365nm): subsp. asaccharolyticus does not fluoresce, subsp. intermedicus shows a limited fluorescence, and subsp. melaninogenicus shows a bright fluorescence. The pigments were isolated as the dimethyl esters of protohaemin and of protoporphyrin and identified by electronic spectroscopy, mass spectrometry and comparisons by t.l.c. Incorporation of delta-aminolaevulinate into these pigments was not detected, nor was porphobilinogen formation observed. Subsp. melaninogenicus grown in the presence of [14C]protohaemin formed [14C]protoporphyrin. This appears to represent a novel biological demetallation. ...
TY - JOUR. T1 - Two New Xylanases with Different Substrate Specificities from the Human Gut Bacterium Bacteroides intestinalis DSM 17393. AU - Hong, Pei-Ying. AU - Iakiviak, M.. AU - Dodd, D.. AU - Zhang, M.. AU - Mackie, R. I.. AU - Cann, I.. N1 - KAUST Repository Item: Exported on 2020-10-01. PY - 2014/1/24. Y1 - 2014/1/24. N2 - Xylan is an abundant plant cell wall polysaccharide and is a dominant component of dietary fiber. Bacteria in the distal human gastrointestinal tract produce xylanase enzymes to initiate the degradation of this complex heteropolymer. These xylanases typically derive from glycoside hydrolase (GH) families 10 and 11; however, analysis of the genome sequence of the xylan-degrading human gut bacterium Bacteroides intestinalis DSM 17393 revealed the presence of two putative GH8 xylanases. In the current study, we demonstrate that the two genes encode enzymes that differ in activity. The xyn8A gene encodes an endoxylanase (Xyn8A), and rex8A encodes a reducing-end ...
The results of a multicenter US survey using the National Committee for Clinical Laboratory Standards currently recommended methodology for measuring in vitro susceptibility of 2673 isolates of Bacteroides fragilis group species were compared from 1997 to 2000. The test panel consisted of 14 antibiotics: 3 carbapenems, 3 β-lactam-β-lactamase inhibitors, 3 cephamycins, 2 fluoroquinolones, clindamycin, chloramphenicol, and metronidazole. Declines in the geometric mean minimum inhibitory concentrations were seen with imipenem, meropenem, ampicillin-sulbactam, and the cephamycins. Increased geometric means were observed with the fluoroquinolones and were usually accompanied by an increase in resistance rates. Bacteroides distasonis shows the highest resistance rates among β-lactam antibiotics, whereas Bacteroides vulgatus shows the highest resistance levels among fluoroquinolones. B. fragilis shows the lowest resistance rates for all antibiotics. All strains were susceptible to chloramphenicol ...
Looking for online definition of Bacteroides capillosus in the Medical Dictionary? Bacteroides capillosus explanation free. What is Bacteroides capillosus? Meaning of Bacteroides capillosus medical term. What does Bacteroides capillosus mean?
We report the single-contig genome sequence of the anaerobic, mesophilic, cellulolytic bacterium, Bacteroides cellulosolvens. The bacterium produces a particularly elaborate cellulosome system, whereas the types of cohesin-dockerin interactions are opposite of other known cellulosome systems: cell-surface attachment is thus mediated via type-I interactions whereas enzymes are integrated via type-II interactions. ...
Electron microscopic studies of laboratory-passaged and animal-passaged isolates of Bacteroides fragilis showed significantly more capsular material around the latter. This observation correlated with increased survival of animal-passaged bacteria in rabbit intraperitoneal chambers and increased resistance to phagocytosis and opsonophagocytic killing by neutrophils. With an initial inoculum of 2.5 x 10(6) colony-forming units/ml, the number of bacteria surviving after incubation for 2 hr with neutrophils and pooled normal human serum was significantly (P less than 0.01) greater for animal-passaged than for laboratory-passaged bacteria. Neutrophil uptake of 14C-labeled animal-passaged and laboratory-passaged bacteria after incubation for 20 min was 45% and 63%, respectively (P less than 0.05). No significant difference in survival was found between animal-passaged and laboratory-passaged isolates of Bacteroides thetaiotaomicron. These findings suggest that on mechanism whereby the capsule of B. ...
Results One hundred and ninety six patients were recruited: 158 CRC patients, 24 adenoma patients and 14 normal colon controls (median age 70; range 35-90). Tumours were staged as 6 T0, 4 T1, 23 T2, 97 T3, 27 T4; 99 N0, 40 N1, 27 N2; 6 M1. No significant differences were seen in diversity or taxonomy between the UK and Czech cohorts. Adenoma and healthy colon control samples were taxonomically indistinct. However, CRCs were characterised by reduced Shannon diversity (p,0.01), with enrichment of organisms including Bacteroides fragilis,Fusobacterium nucleatum and under-representation of Bacteroides vulgatus,Bacteroides uniformisandFaecalibacterium prausnitzii (all q,0.01). Furthermore, we found a significant progressive reduction in the expression of Bacteroides vulgatus with advancing T stage and a corresponding increase in Fusobacterium nucleatum expression (figure). Additionaly, samples from patients with T4 tumours and cancers expressing histological and molecular markers of poor prognosis ...
A notable feature of this and other clone library analyses from G.I. environments is the number of sequences that do not cluster with any known species. Sequences from nonruminant feces fell within the genera Bacteroides and Prevotella, while most sequences from ruminant hosts did not cluster with any known species. Both the range of sequence identity with the closest known species (87 to 91%) and the interclade identity range (81 to 94%) suggest that taxonomic diversity exists among ruminant sequences. Representatives of these groups must be isolated before phenotypic characterization or classification can be determined.. Within the genus Bacteroides, cloned sequences were closely related to known species, reflecting the greater number of cultivated representatives in this genus. This in turn reflects a greater emphasis placed on the study of the human fecal flora relative to other animal hosts. Many cultivated Bacteroides species, particularly B. vulgatus, B. uniformis, B. thetaiotaomicron, ...
Looking for online definition of corpus adiposum buccae in the Medical Dictionary? corpus adiposum buccae explanation free. What is corpus adiposum buccae? Meaning of corpus adiposum buccae medical term. What does corpus adiposum buccae mean?
ID BATHE1_1_PE75 STANDARD; PRT; 96 AA. AC BATHE1_1_PE75; A9ILJ3; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE RecName: Full=2,3,4,5-tetrahydropyridine-2,6-dicarboxylate DE N-succinyltransferase; EC=2.3.1 117;AltName: Full=Tetrahydrodipicolinate DE N-succinyltransferase; Short=THDP succinyltransferase; Short=THP DE succinyltransferase; Short=Tetrahydropicolinate succinylase; DE (BATHE1_1.PE75). GN Name=dapD; OrderedLocusNames=BT_0075; OS BACTEROIDES THETAIOTAOMICRON VPI-5482. OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; OC Bacteroides. OX NCBI_TaxID=226186; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS BATHE1_1.PE75. CC Bacteroides thetaiotaomicron VPI-5482 chromosome, complete genome. CC sequence. CC -!- ANNOTATIONS ORIGIN:DAPD_BART1 CC -!- CATALYTIC ACTIVITY: Succinyl-CoA + (S)-2,3,4,5-tetrahydropyridine- CC 2,6-dicarboxylate + H(2)O = CoA + ...
In this study, the genetic element flanking the BfPAI in ETBF 86-5443-2-2 and a related genetic element in NCTC 9343 were identified and characterized. The results suggest that these genetic elements are members of a new family of CTns not described previously. These putative CTns, designated CTn86 and CTn9343, for ETBF 86-5443-2-2 and NCTC 9343, respectively, differ from previously described Bacteroides species CTns in a number of ways. These new transposons do not carry tetQ, and the excision and formation of circular intermediates are not regulated by tetracycline; they are predicted to have a different mechanism of transposition; and their sequences have very limited sequence homology with CTnDOT or other described CTns.. Initial alignment results with NCTC 9343 (pattern III) and 638R (pattern II) sequences indicated that CTn9343 was ∼80 kb in length; however, further colony blot hybridizations, PCR, and sequence analysis determined that the real ends of CTn9343 define a genetic element of ...
The pentose phosphate pathway is a process of glucose turnover that produces NADPH as reducing equivalents and pentoses as essential parts of nucleotides. There are two different phases in the pathway. One is irreversible oxidative phase in which glucose-6P is converted to ribulose-5P by oxidative decarboxylation, and NADPH is generated [MD:M00006]. The other is reversible non-oxidative phase in which phosphorylated sugars are interconverted to generate xylulose-5P, ribulose-5P, and ribose-5P [MD:M00007]. Phosphoribosyl pyrophosphate (PRPP) formed from ribose-5P [MD:M00005] is an activated compound used in the biosynthesis of histidine and purine/pyrimidine nucleotides. This pathway map also shows the Entner-Doudoroff pathway where 6-P-gluconate is dehydrated and then cleaved into pyruvate and glyceraldehyde-3P [MD:M00008 ...
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Two novel acharan sulfate lyases (ASL1 and ASL2: no EC number) have been purified from Bacteroides stercoris HJ-15 which was… Expand ...
To the Editor.-Eikenella corrodens is a facultative gramnegative bacillus that is a normal inhabitant of the human mouth. Eikenella corrodens infection is relat
Ignore hits to P00634 when looking for other hits (alkaline phosphatase (EC 3.1.3.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.2; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.58; EC 3.1.3.9; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1). alkaline phosphatase (EC 3.1.3.1; EC 3.1.3.75; EC 3.1.3.41; EC 3.1.3.74; EC 3.1.3.99; EC 3.1.3.5; EC 3.1.3.6; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.60; EC 3.1.3.15; EC 3.1.3.102; EC 3.1.3.2; EC 3.1.3.11; EC 3.1.3.20; EC 3.1.3.19; EC 3.1.3.3; EC 3.6.1.25; EC 3.6.1.1; EC 3.1.3.58; EC 3.1.3.23; EC 3.1.3.38; EC 3.1.3.18; EC 3.1.3.68; EC 3.9.1.1 ...
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Summary The presence of capsulate Bacteroides spp. and anaerobic gram-positive cocci was investigated in pus specimens from 182 children with chronic orofacial infections or abscesses and in pharyngeal swabs from 26 children without inflammation. Of 216 Bacteroides spp. and anaerobic cocci isolated from clinical infections, 170 (79%) were capsulate, compared with 34 (35%) of 96 isolates from normal pharyngeal flora (p|0.001). The commonest organisms found to be capsulate more often from infected patients than from controls belonged to the B. melaninogenicus group. The possible evolution of encapsulation in these organisms and their importance in mixed orofacial infections are discussed.
[Biological activity of Bacteroides fragilis].: The investigation was performed with 9 strains of B. fragilis isolated from pregnant women in their 38th week of
Intestinal bacteria are linked to our diet. When participants in this last Danish study were divided by their level of intestinal bacteria, it was found that people with a high proportion of Prevotella bacteria compared to the bacteria Bacteroides lost 3.5 kg more in 26 weeks, they ate a diet composed of the principles of the New Nordic Diet compared to those who consumed a Danish average diet.. Subjects with a low proportion of Prevotella bacteria compared to Bacteroides did not lose any extra weight by following this new Nordic Diet. Overall, about 50% of the population has a high proportion of Prevotella bacteria compared to Bacteroides bacteria.. The study shows that only about half of the population will lose weight if it eats according to the Danish national dietary guidelines - and eats more fruits, vegetables, fiber and whole grains. The other half of the population does not seem to benefit from this change of regime. These people should focus on other feeding recommendations and ...
Author(s): Ngo, Alice; Fong, Kai T; Cox, Daniel L; Chen, Xi; Fisher, Andrew J | Abstract: Uridine 5-diphosphate-N-acetylglucosamine (UDP-GlcNAc) acyltransferase (LpxA) catalyzes a reversible reaction for adding an O-acyl group to the GlcNAc in UDP-GlcNAc in the first step of lipid A biosynthesis. Lipid A constitutes a major component of lipopolysaccharides, also referred to as endotoxins, which form the outer monolayer of the outer membrane of Gram-negative bacteria. Ligand-free and UDP-GlcNAc-bound crystal structures of LpxA from Bacteroides fragilis NCTC 9343, the most common pathogenic bacteria found in abdominal abscesses, have been determined and are presented here. The enzyme crystallizes in a cubic space group, with the crystallographic threefold axis generating the biological functional homotrimer and with each monomer forming a nine-rung left-handed β-helical (LβH) fold in the N-terminus followed by an α-helical motif in the C-terminus. The structure is highly similar to LpxA from other
Gram-negative bacteria naturally produce and secrete nanosized outer membrane vesicles (OMVs). In the human gastrointestinal tract, OMVs produced by commensal Gram-negative bacteria can mediate interactions amongst host cells (including between epithelial cells and immune cells) and maintain microbial homeostasis. This OMV-mediated pathway for host-microbe interactions could be exploited to deliver biologically active proteins to the body. To test this we engineered the Gram-negative bacterium Bacteroides thetaiotaomicron (Bt), a prominent member of the intestinal microbiota of all animals, to incorporate bacteria-, virus- and human-derived proteins into its OMVs. We then used the engineered Bt OMVs to deliver these proteins to the respiratory and gastrointestinal (GI)-tract to protect against infection, tissue inflammation and injury. Our findings demonstrate the ability to express and package both Salmonella enterica ser. Typhimurium-derived vaccine antigens and influenza A virus (IAV)-derived ...
Sheridan , P O , Martin , J C , Lawley , T D , Browne , H P , Harris , H M B , Bernalier-Donadille , A , Duncan , S H , OToole , P W , Scott , K P & Flint , H J 2016 , Polysaccharide utilization loci and nutritional specialization in a dominant group of butyrate-producing human colonic Firmicutes Microbial Genomics , vol 2 , no. 2 , 43 , pp. 1-16 . DOI: 10.1099/mgen. ...
Our laboratories are particularly well placed to conduct this interdisciplinary study. The PI, Dr Abratt at the University of cape Town, has specialised anaerobic growth facilities available, and a proven track record of research in the areas of anaerobic microbiology and molecular genetics, particularly of the Bacteroides nitrogen metabolism.. In addition, we have established the capability to determine protein structure in Africa for the first time through a grant by the Carnegie Corporation of New York to B.T. Sewell (co-investigator). This grant has enabled the creation of the joint UCT/UWC Masters programme in Structural Biology and has, in particular led to the establishment of a facility for protein X-ray crystallography at the University of the Western Cape and the establishment of a protein NMR facility at the University of Stellenbosch. Six masters students have been recruited to the programme in its first year (2003). Each student cohort will take two years, including a year of ...
Dynamics of the metallo-beta-lactamase from Bacteroides fragilis in the presence and absence of a tight-binding inhibitor.: A significant determinant for the br
During cultivation on a mixture of xylose and glucose, Bacteroides xylanolyticus X5-1 showed neither diauxic growth nor a substrate preference. Xylose-limited continuous-culture cells were able to consume xylose and glucose both as single substrates and as mixed substrates without any lag phase. When glucose was the growth-limiting substrate, the microorganism was unable to consume xylose. However, in the presence of a small amount of glucose or pyruvate, xylose was utilized after a short lag phase. In glucose-limited cells, xylose isomerase was present at low activity but xylulose kinase activity could not be detected. On addition of a mixture of xylose and glucose, xylose isomerase was induced immediately and xylulose kinase was induced after about 30 min. The induction of the two enzymes was sensitive to chloramphenicol, showing de novo synthesis. Xylose uptake in glucose-grown cells was very low, but the uptake rate could be increased when incubated with a xylose-glucose mixture. The ...
The treatment for Eikenella Corrodens caused infections depends upon the severity of the disorder as well as the condition of the patient. Generally, two treatment options are suggested by the professionals which include the following;. Medication therapy:Usually the antibiotics are recommended for these infections which may include; penicillins, cephalosporins, or tetracyclines. As well as these are considered to be highly vulnerable to the fluoroquinolones (ciprofloxacin) but the adequate in-vivo shreds of evidence for this class have not been provided yet.. Meanwhile, it is stated that this bacterial body is highly resistant to the administration of a few drug products involving macrolides (for example erythromycin), metronidazole and clindamycin.. Surgical therapy: Depending upon the severity of the infection, some cases may require a surgical procedure. This technique involves the surgical drainage from the affected area and afterward, the patient is recommended for the antibacterial ...
ID R7NV87_9BACE Unreviewed; 354 AA. AC R7NV87; DT 24-JUL-2013, integrated into UniProtKB/TrEMBL. DT 24-JUL-2013, sequence version 1. DT 25-OCT-2017, entry version 13. DE RecName: Full=V-type ATP synthase subunit I {ECO:0000256,RuleBase:RU361189}; GN ORFNames=BN821_00066 {ECO:0000313,EMBL:CDF16586.1}; OS Bacteroides sp. CAG:98. OC Bacteria; Bacteroidetes; Bacteroidia; Bacteroidales; Bacteroidaceae; OC Bacteroides; environmental samples. OX NCBI_TaxID=1262754 {ECO:0000313,EMBL:CDF16586.1, ECO:0000313,Proteomes:UP000018115}; RN [1] {ECO:0000313,EMBL:CDF16586.1, ECO:0000313,Proteomes:UP000018115} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=MGS:98 {ECO:0000313,Proteomes:UP000018115}; RA Nielsen H.B., Almeida M., Juncker A.S., Rasmussen S., Li J., RA Sunagawa S., Plichta D., Gautier L., Le Chatelier E., Peletier E., RA Bonde I., Nielsen T., Manichanh C., Arumugam M., Batto J., RA Santos M.B.Q.D., Blom N., Borruel N., Burgdorf K.S., Boumezbeur F., RA Casellas F., Dore J., Guarner F., ...
Trillions of microorganisms inhabit mucosal surfaces of the human body. Despite increasing evidence of their impact on human health, many of the molecular mechanisms underlying hostmicrobial interactions (HMI) are poorly understood. To contribute to our understanding of HMI at mucosal surfaces, we investigated the novel family of M60-like/PF13402 domain-containing proteins and their putative functional partners. M60-like domains are shared by proteins from several mucosal microbes including two important human mucosal microbes; the bacterial mutualist Bacteroides thetaiotaomicron and the protist pathogen Trichomonas vaginalis, suggesting these proteins are important for interaction with the mucosal layer. We initially tested our hypothesis that these are glycoprotein-targeted metal dependent proteases in both these organisms. The three M60-like domains of B. thetaiotaomicron proteins (BT4244, BT3015 and BT4272) exhibited mucin protease activity. This proteolytic activity was shown to be ...
The results of the study demonstrated a decrease in the sensitivity of bacteria in the B.fragilis group to modern anti-anaerobic drugs: cefoxitin, clindamycin, moxifloxacin, imipenem, piperacillin / tazobactam and metronidazole.
Accepted name: 4-O-β-D-mannosyl-D-glucose phosphorylase. Reaction: 4-O-β-D-mannopyranosyl-D-glucopyranose + phosphate = D-glucose + α-D-mannose 1-phosphate. Glossary: 4-O-β-D-mannopyranosyl-D-glucopyranose = β-D-mannopyranosyl-(1→4)-D-glucopyranose. Other name(s): mannosylglucose phosphorylase. Systematic name: 4-O-β-D-mannopyranosyl-D-glucopyranose:phosphate α-D-mannosyltransferase. Comments: This enzyme forms part of a mannan catabolic pathway in the anaerobic bacterium Bacteroides fragilis NCTC 9343.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, CAS registry number: References:. 1. Senoura, T., Ito, S., Taguchi, H., Higa, M., Hamada, S., Matsui, H., Ozawa, T., Jin, S., Watanabe, J., Wasaki, J. and Ito, S. New microbial mannan catabolic pathway that involves a novel mannosylglucose phosphorylase. Biochem. Biophys. Res. Commun. 408 (2011) 701-706. [PMID: 21539815]. ...
Opens the Highlight Feature Bar and highlights feature annotations from the FEATURES table of the record. The Highlight Feature Bar can be used to navigate to and highlight other features and provides links to display the highlighted region separately. Links in the FEATURES table will also highlight the corresponding region of the sequence. More... ...
免疫過敏:無菌老鼠一出生就被放入無菌箱中,腸道中沒有半隻腸道菌,這種無菌鼠生活在無菌箱中確實愉快,吃得比一般有菌鼠多,體脂肪反而低,活得又比有菌鼠久,可惜免疫系統不健全,出了無菌箱便無法生存。許多研究都顯示無菌鼠,或以抗生素打亂腸道菌的老鼠,血清中免疫球蛋白IgE濃度顯著高於正常鼠,全身呈現發炎過敏現象;如果給無菌鼠餵食特定的腸道菌,例如脆弱擬桿菌(Bacteroides fragilis),則有助於減輕發炎與過敏現象。許多科學家懷疑,近年來自體免疫疾病發生率快速上升,與抗生素濫用而導致許多人腸道菌嚴重失衡有關 ...
The researchers now plan to expand the application of their tools to different species of Bacteroides. That is because the microbial makeup of the gut varies from person to person, meaning that a particular species might be the dominant bacteria in one patient, but not in others.. We aim to expand our genetic toolkit to a wide range of bacteria that are important commensal organisms in the human gut, Lu says.. The concept of using microbes to sense and respond to signs of disease could also be used elsewhere in the body, he adds.. In addition, more advanced genetic computing circuits could be built upon this genetic toolkit in Bacteroides to enhance their performance as noninvasive diagnostics and therapeutics.. For example, we want to have high sensitivity and specificity when diagnosing disease with engineered bacteria, Lu says. To achieve this, we could engineer bacteria to detect multiple biomarkers, and only trigger a response when they are all present.. Tom Ellis, group leader of the ...
GH105 enzymes do not act via a typical Koshland retaining or inverting mechanism [8], rather the current proposed mechanism of action for these enzymes is hydrolysis through syn-hydration of the double bond between the C-4 and C-5 carbons of the enopyranuronosyl residue of their substrate [5]. This hydration reaction forms a hemiketal that undergoes spontaneous rearrangement to form an intermediate hemiacetyl, which undergoes further rearrangement resulting in the breakage of the bond to the neighbouring saccharide (at the +1 subsite of the enzyme) of the polymer. This mechanism was initially theorized based on the oligosaccharide and amino acid arrangement in a substrate-bound crystal structure [6], but has been confirmed through kinetic isotope effects and NMR analysis in the highly related unsaturated glucuronyl hydrolase GH88 family [3, 9]. The kinetics for three enzymes from the GH105 family have been determined, two from Bacillus subtilis and one from Bacteriodes thetaiotaomicron. YteR ...
Agudelo, RM et al. Monitoring bacterial faecal contamination in waters using multiplex real-time PCR assay for Bacteroides spp. and faecal enterococci. Water SA (Online), Jan 2010, vol.36, no.1, p.127-132. ISSN 1816- ...
Marvin5.2 introduced the enumeration of homology groups. Homology groups are R-groups represented as pseudo atoms - with the names covering a set of R-groups either built-in or user-defined.. ...