TY - JOUR. T1 - High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E.coli lysogen. AU - Gunther, Edward. AU - Murray, Noreen E.. AU - Glazer, Peter M.. PY - 1993/8/11. Y1 - 1993/8/11. UR - http://www.scopus.com/inward/record.url?scp=0027236017&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027236017&partnerID=8YFLogxK. U2 - 10.1093/nar/21.16.3903. DO - 10.1093/nar/21.16.3903. M3 - Article. C2 - 8396240. AN - SCOPUS:0027236017. VL - 21. SP - 3903. EP - 3904. JO - Nucleic Acids Research. JF - Nucleic Acids Research. SN - 0305-1048. IS - 16. ER - ...
We have examined the impact of DNA heterologies on the packaging of lambda DNA in vitro. Heterology-containing DNA molecules were constructed by denaturing and reannealing a mixture of DNA from cI+ phage and DNA front phage carrying small insertion or deletion mutations in the cI gene. We found that molecules with heterologies of up to 19 base pairs (bp) can be packaged as viable heterozygous phage with approximately the same efficiency as molecules with a base pair mismatch. In contrast, with a heterology of 26-bp heterozygous plaque formers are rare. In principle, the absence of cI heterozygotes among packaged phage may be due either to a failure to encapsulate the DNA or a failure to inject the packaged DNA on infection. Southern blot analysis of DNA isolated from packaged phage indicates that DNA harboring a 26-bp heterology is almost completely absent in packaged phage. Thus, an upper limit has been established for the size of heterology that can be accommodated by the packaging apparatus ...
The DNA in its circular form contains 48,502 base-pairs...Bacteriophage lambda DNA in its circular form contains 48,502 base-pairs and codes for about 60 proteins. According to abstract phage Lambda has 46 clearly defined ORFs and another ~20 putative ORFs, totaling ~66ORFs (~60 proteins according to p.730 2nd paragraph). The number of bases is for the double stranded circular chromosome. 48,514 bp according to Lewin, Genes VIII, 2004, p.335 fig.12.11 ...
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Experiments using phage lambda provided early insights into important molecular mechanisms, including genetic recombination and the control of gene expression. Before recombinant DNA technology, the use of lambda, most particularly lambda transducing phages, illustrated the importance of cloning bacterial genes, already providing some insight into how to use cloned genes to advantage. Subsequently, lambda made significant contributions to recombinant DNA technology, including the early generation of genomic and cDNA libraries. More recently, lambda genes associated with recombination have enabled techniques referred to as recombineering to be developed. These techniques permit the refined manipulation, including mutation, of foreign genes in Escherichia coli and their subsequent return to the donor organism. ...
TY - JOUR. T1 - Bacteriophage lambda-based expression vectors. AU - Christensen, A. C.. PY - 2001/6/28. Y1 - 2001/6/28. N2 - Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadvantages associated with its mode of growth and its size. Some lambda vectors have been designed to be readily converted into plasmids or phagemids, and there are a variety of promoters and fusions that can be used to drive expression of foreign genes. Screening lambda libraries with antibodies or ligands is a powerful way of identifying novel genes.. AB - Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque ...
Until recently, a relatively simple model was proposed for the biological function of the amino-terminal domain of the lambda Int. The high-affinity amino-terminal domain binds to the arm-type sequence to deliver the low-affinity core-binding domain of Int to the core-type site, where actual cleavage and rejoining occur (9, 12, 13). Simultaneous binding to the two different DNA sites by a single Int molecule is further facilitated by accessory proteins that bind and bend the sites between the arm-type and the core-type sequences (9, 13).. Several recent studies, however, revealed more elaborate roles of the amino-terminal domain of the Int protein in addition to the architectural role. The amino-terminal domain is implicated as a region involved in protein-protein interactions between Int molecules during intasome formation (8). Some amino acid substitutions in one domain may alter the structure of the other domain through domain-domain communication. A few amino acid substitutions in the HK022 ...
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href="http://www.uniprot.org/manual/proteomes_manual">proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
Rx Biosciences prepares genomic libraries in the lambda phage vector such as lambda Dash II, EMBL3, and others. We specialize in the construction of libraries from micro- quantities of the specimen such as trace amount of genomic DNA, chromosomes, uncultured environmental microbes, or base-modified phages. Highly methylated DNA, contamination with polysaccharides or phenolic compounds or restriction enzyme resistant DNA samples are successfully used to generate high quality libraries. The libraries are provided to the customer after rigorous Q.C. testing.. ...
Lambda phage --, lambda bacteriophage (Science: virology) bacterial dna virus, first isolated from E. Coli. Its structure is similar to that of the t even phages. lambda genetic material consists of a double-stranded dna molecule with 5 twelve-base-pair sticky ends, known as cos sites, which permit circularisation of the dna molecule. It shows a lytic cycle and a lysogenic cycle and studies on the control of these alternative cycles have been very important for our understanding of the regulation of gene transcription. It is used as a cloning vector, accommodating fragments of dna up to 15 kilobase pairs long. For larger pieces, the cosmid vector was constructed from its ends. ...
Date: Sat, 1 Oct 2016 16:10:40 -0400 Reply-To: Susan Gottesman [[email protected]] From: Susan Gottesman [[email protected]] Subject: Lambda lunch update To: [[email protected]] List-Help: [http://list.nih.gov/cgi-bin/wa.exe?LIST=LAMBDA_LUNCH-L], [mailto:[email protected]?body=INFO%20LAMBDA_LUNCH-L] List-Unsubscribe: [mailto:[email protected]] List-Subscribe: [mailto:[email protected]] List-Owner: [mailto:[email protected]] List-Archive: [http://list.nih.gov/cgi-bin/wa.exe?LIST=LAMBDA_LUNCH-L] 10/6/16*: Martin Schmeing (McGill) "Structural and functional studies of nonribosomal peptide synthetase megaenzymes" (G. Storz) 10/7/16: Noon, Bldg. 6A/Rm. 4A05: Paul Schimmel (Scripps)"New Deep Biology of Human tRNA Synthetases and Connection to Disease" (DDB Postdoctoral Fellows Speaker Series; S. Mattijssen) 10/11/16: 1-2 PM, Bldg. 40, Rm. 1201/1203, Bryan Krantz (U. MD, Baltimore) "Peptide- and ...
Date: Wed, 26 Oct 2016 09:25:03 -0400 Reply-To: Susan Gottesman [[email protected]] From: Susan Gottesman [[email protected]] Subject: Lambda lunch update, Oct. 26 To: [[email protected]] List-Help: [http://list.nih.gov/cgi-bin/wa.exe?LIST=LAMBDA_LUNCH-L], [mailto:[email protected]?body=INFO%20LAMBDA_LUNCH-L] List-Unsubscribe: [mailto:[email protected]] List-Subscribe: [mailto:[email protected]] List-Owner: [mailto:[email protected]] List-Archive: [http://list.nih.gov/cgi-bin/wa.exe?LIST=LAMBDA_LUNCH-L] 10/27/16*: Robert Trachman (A. Ferre-DAmare lab) "Structural Basis for fluorescence enhancement by an RNA aptamer" 11/3/16*: Anca Segall (San Diego State; on sabbatical at NCBI) (Note: Arkady Mustaev moved to 12/8/16) 11/10/16*: Jeremy Bird (Ebright/Nickels labs, Rutgers University)"The mechanism of RNA 5 capping with NAD+, NADH and desphospo-Coenzyme A" (S. Gottesman, S. Wickner) 11/17/16*: ...
Developmental systems are controlled by modulating gene expression in response to internally programmed signals as well as to external signals. Our laboratory is interested in studying the molecular interactions and the signaling that occur to regulate gene expression. We exploit the genetic systems available in Escherichia coli, its plasmids, and its viruses (e.g., bacteriophage lambda) to help us understand (1) gene regulation at the levels of transcription initiation and elongation, and translation initiation, and (2) cell growth and cell cycle control signals.. RECOMBINEERING: Recently, we have developed an in vivo cloning and gene modification system using lambda Red-mediated homologous recombination with short (,50 bp) homologies. This new technology called recombineering is being used. It is a great tool in E. coli for genetic modification. We have provided recombineering strains to more than 3000 labs. Well over half of these labs work on eukaryotic model systems like mouse and ...
Misc.Comments : Deposited by: Ichiro N. Maruyama Restriction digests of the clone give the following sizes (kb): HindIII--24.1, 17.6; EcoRI--22.0, 20.0; BamHI--24.8, 16.9; XbaI--32.7,9.0; NotI--41.7. (ATCC staff) Vector useful for constructing cDNA libraries. Permits positive selection for inserts using the Spi- phenotype, and excision of phagemid by lox/cre site-specific recombination. [1] To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046[pCRE1], ATCC 77368) and select for ampicillin resistance. The pMGU product is 4.185 kb. [1] The order of the major features in the cloning region of the lambda vector is: lambda J - SmaI - SalI - loxP - EcoRI - M13 ori - ampR - pMB1 ori - HindIII - 3gam/BamHI/5gam - XhoI - loxP - SalI - lambda N. [1] Inserts can be amplified using the following primers flanking the BamHI cloning site: upstream 5-AAGAGGCAGAACTGGCAG-3 and downstream 5-ATCGATGCATAGCGATTC-3. [1] Efficiency of phagemid recovery is ...
An entire gene containing its coding region with its intron as well as its regulatory region such as a promoter or terminator can be cloned from a chromosome by screening of genomic library which is constructed in phage vector or plasmid vector in an appropriate host, by using a partial DNA fragment obtained by degenerate PCR as described above as a probe after it was labeled. Generally, E. coli as a host strain and E. colt vector, a phage vector such as lambda phage vector, or a plasmid vector such as pUC vector is often used in the construction of library and a following genetic manipulation such as a sequencing, a restriction digestion, a ligation and the like. In this invention, an EcoRl genomic library of P. rhodozyma was constructed in the derivatives of lambda vector, lambda gtll. An insert size, what length of insert must be cloned, was determined by the Southern blot hybridization before a construction of a library. In this invention, a DNA which was used for a probe was labeled with ...
Common plasmids are simple DNA molecules which contain a few genes and regulatory elements. Most viral genomes are more complex. For example, the genome of phage lambda contains approximately 50 genes. About 4,000 genes are present in the E. coli genome while there is approximately 1,000 times more DNA in the genome of a mammal. This progression in genome complexity is the topic of this exercise. Here, students compare the electrophoretic patterns of restriction digests of a plasmid, phage lambda DNA, and cow DNA from thymus and kidney as shown in the figure below. The exercise serves as a good introduction for determining the size of DNA molecules and provides an appreciation for the complexity of genomes from different organisms.. ...
of a series of informal workshops initiated by Makoto Kanazawa. This series is mostly concerned with formalisms independently defined by Reinhard Muskens and Philippe de Groote: lambda grammars or abstract categorial grammars. This workshop more particularly focuses on: ...
begin{aligned}& \bigl\vert T_{1}(u_{2},v_{2}) (t)-T_{1}(u_{1},v_{1}) (t)\bigr\vert \\& \quad \leq \biggl\vert A_{1}(t) \biggl\{ a\bigl(\lambda_{2} \xi-1+e^{-\lambda_{2}\xi}\bigr) \biggl[ \int_{0}^{1}e^{-\lambda _{2}(1-s)}\bigl[Q(u_{2},v_{2}) (s)-Q(u_{1},v_{1}) (s)\bigr]\,ds \\& \qquad {}-b \int_{0}^{\eta}e^{-\lambda_{1}(\eta -s)}\bigl[P(u_{2},v_{2}) (s)-P(u_{1},v_{1}) (s)\bigr]\,ds \biggr] \\& \qquad {}-\bigl(\lambda_{2}-1+e^{-\lambda_{2}}\bigr) \biggl[a \int_{0}^{\xi}e^{-\lambda_{2}(\xi -s)}\bigl[Q(u_{2},v_{2}) (s)-Q(u_{1},v_{1}) (s)\bigr]\,ds \\& \qquad {}- \int_{0}^{1}e^{-\lambda_{1}(1-s)}\bigl[P(u_{2},v_{2}) (s)-P(u_{1},v_{1}) (s)\bigr]\,ds \biggr]\biggr\} \\& \qquad {}+ \int_{0}^{t}e^{-\lambda_{1}(t-s)}\bigl[P(u_{2},v_{2}) (s)-P(u_{1},v_{1}) (s)\bigr]\,ds\biggr\vert \\& \quad \leq A_{1} \biggl\{ ,a,\bigl(\lambda_{2} \xi-1+e^{-\lambda_{2}\xi}\bigr) \biggl[\bigl(m_{2}\Vert u_{2}-u_{1}\Vert +n_{2}\Vert v_{2}-v_{1}\Vert \bigr) \int_{0}^{1}e^{-\lambda_{2}(1-s)}(Q{\mathbf{1}}) (s)\,ds \\& \qquad ...
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Stříkačková pumpa je k dispozici ve dvou provedeních LAMBDA VIT-FIT (možnost přepínaní mezi 80 N a 300 N) a vysokotlaká LAMBDA VIT-FIT HP (možnost přepínaní mezi 160 N a 600 N).. Každá z těchto verzí navíc umožňuje volbu rychlosti posuvu a to buď v režimu SLO (od 0,02 do 20 mm/min.) nebo v režimu FAS (od 0,08 do 80 mm/min.). LAMBDA VIT-FIT stříkačkové pumpy umožňují jednoduše použít libovolné stříkačky od 5 μl do 150 ml bez přídavných adaptérů, čímž odpadá pracná manipulace nebo dokonce nutnost mít více pump podle potřebného objemu stříkačky.. ...
Abstract: The top-Higgs system, consisting of top quark LH doublet, RH singlet) and Higgs boson kinetic terms, with gauge fields set to zero, has an exact (modulo total divergences) symmetry where both fermion and Higgs fields are shifted and mixed in a supersymmetric fashion. The full Higgs-Yukawa interaction and Higgs-potential, including additional \sim 1/\Lambda^2 NJL-like interactions, also has this symmetry to {O}1/\Lambda^4), up to null-operators. Thus the interaction lagrangian can be viewed as a power series in 1/\Lambda^2. The symmetry involves interplay of the Higgs quartic interaction with the Higgs-Yukawa interaction and implies the relationship, \lambda = \half g^2 between the top--Yukawa coupling, g, and Higgs quartic coupling, \lambda, at a high energy scale \Lambda \gta few TeV. We interpret this to be a new physics scale. The top quark is massless in the symmetric phase, satisfying the Nambu-Goldstone theorem. The fermionic shift part of the current is \propto (1-H^\dagger ...
Abstract: The top-Higgs system, consisting of top quark (LH doublet, RH singlet) and Higgs boson kinetic terms, with gauge fields set to zero, has an exact (modulo total divergences) symmetry where both fermion and Higgs fields are shifted and mixed in a supersymmetric fashion. The full Higgs-Yukawa interaction and Higgs-potential, including additional \sim 1/\Lambda^2 NJL-like interactions, also has this symmetry to O(1/\Lambda^4), up to null-operators. Thus the interaction lagrangian can be viewed as a power series in 1/\Lambda^2. The symmetry involves interplay of the Higgs quartic interaction with the Higgs-Yukawa interaction and implies the relationship, \lambda = \half g^2 between the top--Yukawa coupling, g, and Higgs quartic coupling, \lambda, at a high energy scale \Lambda ,= few TeV. We interpret this to be a new physics scale. The top quark is massless in the symmetric phase, satisfying the Nambu-Goldstone theorem. The fermionic shift part of the current is \propto (1-H^\dagger ...
The heart of LAMBDA Pumps (or LAMBDA DOSER) is a stepping motor controlled by a generator of electrical impulses. After each impulse the motor turns by one step. This movement is transmitted to the rotor, which displaces a small amount of liquid in the direction of flow by compression of the tubing. The integrator registers impulses received and transforms them into a direct current. The voltage can be measured or recorded by common recorders or voltmeters ...
A pair of ultra-micro cells enables easy and reproducible analysis of very small sample volumes, down to 30 µL, and the reference material set provides validation of the ordinate and wavelength accuracy of your Lambda 25. The liquid sipper enables fast, in-situ liquid sampling directly from the container. For use with the LAMBDA UV/Vis Systems. ...
Ah lambdas. If youve used any functional languages, python, ruby, or C# (or many other languages), you are probably familiar with the concept of lambdas. Ho...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
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polysyms mu0 lambda; % Use mu0 because mu is a Matlab toolbox command v = polysym(v,[1 2]); fname = polysym(f,[1 2]); A = [1 2; 2 4]; B = [4 -2; -2 1]; fval = mu0*A*v. - lambda*B*v.; poly_system = BertiniLab(function_def,fname(:),function_def,fval(:), ... hom_variable_group,{[mu0 lambda],v}); poly_system = solve(poly_system); sols = poly_system.match_solutions(raw_solutions,mu0,lambda,v ...
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For over 50 years we have remained at the forefront of research and innovation. Now, we have drawn on our experience to bring you the LAMBDA XLS+ spectrophotometer, the instrument you can depend on to meet the demands of your busy laboratory. ...
Sets the lambda scaling factor. When an improved solution is found, lambda is multiplied by this factor. When a poorer solution is found, lambda is divided by this factor. The smaller the factor, the more rapidly the Levenberg-Marquardt algorithm shifts to the standard Hessian method. The factor must satisfy 0 < _factor < 1.0. Default is 0.1 ...
Thanks for telling me that you could not get my message, I hope this work better... so my question was: I built a population matrix to which I applied the fonction eigen in order to find the main parameters about my population. I know that the first eigen value correspond to lambda or exponential growth rate of my population. My problem is that I want to have the 95% confidence interval of the specific lambda (1.056 in the case). Is there a way to do that? Are the other eigen value shown in the output could help me doing it. I would very appreciate any help. Thanks for your time $values [1] 1.0561867+0.0000000i 0.0749653+0.5249157i 0.0749653-0.5249157i [4] 0.4498348+0.0795373i 0.4498348-0.0795373i -0.3357868+0.0000000i $vectors [1,] -0.72849129+0i -0.11058308+0.3293511i -0.11058308-0.3293511i 0.00244042+0.03012017i 0.00244042-0.03012017i [2,] -0.41384232+0i 0.35124594+0.1765638i 0.35124594-0.1765638i 0.01004458+0.03839895i 0.01004458-0.03839895i [3,] -0.27427879+0i 0.29630718-0.4260863i ...
The OMNICOLL fraction collector provides a signal (~ 9 V) which is used by the communication module (art. no. 6911) to switch the pump off while moving from one fraction position to the other and at the end of the run. Therefore, there is no spilling between fractions ...
The most general calling discipline which can exist is to pass promises as arguments and let the called function decide what to do with them, and pass promises as return values and let the call site decide what to do with them. A promise, as you will recall, is comprised of the expression and the environment in which the expression appeared.. If that were it, and the promises were treated conventionally, it would just be a lazy language. The generality of macrology, etc, comes from allowing the called routine (or the call site in the case of return promises) to *abuse* the promises to varying degrees. Things you can do with a promise, in order from least to most semantically general/abusive, are: "Force" -- the conventional treatment of a promise. The expression is evaluated in the provided environment and the promise is replaced with the value returned from it. You can only "Force" a promise once. With forcing, the language is still referentially transparent.. "Evaluate" -- That is, you ...
In one sense, this is light enough that I feel bad posting it to LtU. In another, though, its entirely relevant: this guy started out inventing a DSL for pen-strokes, combinations of them, and relations between these combinations, and ended up with a simple OCR-based 4-function calculator. This was one of the cleverest bits of just-because programming language work and contrived abstraction that Ive seen in a long time. Sample:. ...
Excellgen Lambda Phage Integrase, Int [EG-40] - Description E coli lambda phage integrase Int is a site-specific tyrosine recombinase which mediates inserts and excises the phage genome into and out of the Escherichia coli chromosome. Lambda phage integrase has been used for LR and BP reactions in Gateway Cloning. Applications in vitro recombination assay Size 100 µg Source E coli overexpressing lambda phage Int
Noreen Murray was one of the architects of the recombinant DNA revolution that transformed the study of biology from the early 1970s. Her particular prowess for genetic manipulation of bacteria and their phage was critical in developing the bacteriophage lambda vectors that were a vital part of the early genetic engineering toolbox. Her skill as a microbial geneticist had earlier become apparent through her work on genetic recombination and complementation in the fungus Neurospora, especially as a postdoctoral researcher at Stanford where her work brought her to the attention of some of the giants of early molecular biology. Back in the UK, first at Cambridge and then, for the bulk of her career, at Edinburgh, she produced a remarkable body of work focused on uncovering the mechanisms and biology of restriction enzymes, and their adaptation as tools underpinning modern biological research and the rise of the biotechnology industry. Much of this work was done in collaboration with her husband Ken ...
ID PHA10 preliminary; circular DNA; SYN; 11309 BP. XX AC ATCC37065; XX DT 01-JUL-1993 (Rel. 7, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli plasmid vector pHA10 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pJS1, pJS17 from pOP203-3 & lambda, kil gene RC pTR12, pTR20 from pJS1 RC pJS3, pJS10, pJS13, pHA1, pHI74, pHN1 from pBR322 & lambda, kil gene RC pHA10 from pHA1 & lambda RC p2824 from Charon 3 & pBR322 RC pKL1 from p2824 & lambda RA Honigman A., Oppenheim A.B., Hohn B., Hohn T.; RT "Plasmid vectors for positive selection of DNA inserts controlled RT by the lambda pL promoter, repressor and antitermination RT function"; RL Gene 13:289-298(1981). XX RN [2] RC pKB155 from pCR11 & lambda, cI gene RC pKB158 from pKB155 & pMB9 RC pKB252 from pKB158 & lac promoter RC [pKB255 from pKB158 & lac promoter] RA Backman K., Ptashne M., Gilbert W.; RT "Construction of plasmids carrying the cI gene of bacteriophage ...
define (ask object message . args) (apply (get-method object message) object args)) (define (get-method object message) (object message)) (define make-object (lambda (name) (lambda (message) (case message ((class) (lambda (self) object)) ((object?) (lambda (self) #t)) ((name) (lambda (self) name)) (else (error "No method")))))) (define make-boat (lambda (name) (let ((super (make-object name)) (floating #t)) (lambda (message) (case message ((class) (lambda (self) boat)) ((boat?) (lambda (self) #t)) ((is-floating?) (lambda (self) floating)) ((sink) (lambda (self) (set! floating #f))) (else (get-method super message))))))) (define make-sail-boat (lambda (name) (let ((super (make-boat name)) (sailing #f)) (lambda (message) (case message ((class) (lambda (self) sail-boat)) ((raise-sail) (lambda (self) (set! sailing #t))) ((lower-sail) (lambda (self) (set! sailing #f))) (else (get-method super message ...
2M7B: The Solution Structures of Two Prophage Homologues of the Bacteriophage lambda Ea8.5 Protein Reveal a Newly Discovered Hybrid Homeodomain/Zinc-Finger Fold.
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Hello, I am interested in a cloning vector which allows direct selection for ,DNA inserts as pCS19 developed from Christian Sengstag (Gene 124,p141-2). ,However, in pCS19 a BamHI site is used for subcloning. Because we want to ,insert Klenow treated PCR fragments we are interested in a corresponding ,vector but containing a blunt cut restriction site for subcloning (has not to ,be a yeast shuttle vector for our purpose). Is such a vector available and ,who may provide us with this. Could you send your answer also directly to my ,e-mail box (hilt at po.uni-stuttgart.de) Thank you. wohi There is a yeast shuttle vector (pWH5) that may cater for your needs that was published by Wright APH, Maundrell K, Heyer W-D, Beach D, Nurse P (1986) Vectors for the construction of gene banks and the integration of cloned genes in S.pombe and S.cerevisiae. Plasmid, 15:156-158. pWH5 contains a tet resistence gene driven by the lambda Pr promoter and it also contains a version of the cI repressor gene containing ...
Genomes of living organisms are comprised of very long DNA molecules. A fundamental question is by what mechanisms are specific loci along these genomes found, with high efficiency and at relevant physiological times. We address this question in the case of horizontal gene transfer processes such as viral transduction and conjugation, which result in the rapid acquisition of new traits in bacteria. We use the infection of E. coli cells by bacteriophage lambda, whose DNA integrates at a unique site into the bacterial genome, when following the lysogenic pathway. To shed light on the mechanisms by which lambda DNA finds its unique integration site, we follow in real time individual lambda DNAs and their integration site within live cells using fluorescent markers, until lysogeny is established, revealing the dynamics of the search process.. ...
In article ,396aas$ki1 at news.service.uci.edu,, rmauk at crick.bio.uci.edu (Rob Mauk) wrote: , , I have isolated lambda gt10 DNA by PEG precipitation followed by organic , extraction and ethanol precipitation, and find that it is almost , completely resistant to digestion by EcoRI and all other enzymes that I , have tested. Proteinase K treatment did not help, nor did additional , extractions and precipitations. Plasmid DNA digests normally with these , enzymes, but if the plasmid is mixed with the lambda DNA, it is rendered , undigestable. Any comments or suggestions regarding the nature of this , contaminant, or solutions to the problem would be greatly appreciated. , Thank you. I have found in many cases that the addition of spermidine to a final concentration of 4 mM in the restriction buffer will allow the enzymes to work. It is an easy test!! Tom Newman Assistant Prof. MSU-DOE Plant Research Laboratory ...
Finck A. (1968) Grenzwerte der Nährelementgehalte in Pflanzen und ihre Auswertung zur Ermittlung des Düngerbedarfs. J. Plant Nutr. Soil Sci. 119: 197-208.. Günther J & Schroeder D (1968) Über den Einfluß von Bodeneigenschaften auf die Aufnahme von radioaktivem Stontium durch Pflanzen. II.: Untersuchungen an Modellböden mit systematisch variierten Merkmalen. J. Plant Nutr. Soil Sci. 120: 78-89.. Günther J & Schroeder D (1968) I.: Über den Einfluß von Bodeneigenschaften auf die Aufnahme von radioaktivem Stontium durch Pflanzen. I.:Untersuchungen an Böden Schleswig-Holsteins mit Weibklee und Weidelgras. J. Plant Nutr. Soil Sci. 119: 216-226.. Hoffmann WE (1966) Über die Regelung der Aufnahme einzelner Mineralstoffe bei der höheren Pflanze. J. Plant Nutr. Soil Sci. 113: 106-112.. Hoffmann WE (1966) Veränderungen der Wurzelaktivität der Pflanze bei der Mineralstoffabgabe an den Sproß durch unterschiedliche Kaliumernährung. J. Plant Nutr. Soil Sci. 113: 120-130.. Schlichting E (1965) ...
On Thu, 9 Mar 2006, Wayne Kelly wrote: , Im having trouble understanding the logic behind the semantics of the , break construct, specifically when used in lamdbas and methods. Im also having that trouble. Its something that is much easier to understand in Perl5, as there is no Proc/Block/Method difference and lambdas always work the same and so do breaks. sub myiterator { my($list,$proc)[email protected]_; for my $i (@$list) { $proc-,($i) } } sub func { my($x)[email protected]_; print $x\n; last; print after\n } my $lambda = sub { my($x)[email protected]_; print $x\n; last; print after\n }; myiterator([1..10],sub {my($x)[email protected]_; print $x\n; last; print after\n}); myiterator([1..10],$lambda); myiterator([1..10],\&func); ------------------8,--------cut-here--------8,------------------ prints this: 1 1 1 perl is so easy. sometimes. _ _ __ ___ _____ ________ _____________ _____________________ ... , Mathieu Bouchard - t :+1.514.383.3801 - http://artengine.ca/matju , Freelance Digital Arts Engineer, Montr l QC Canada ...
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