The genomic organization of the chromosomal cps region that is responsible for capsular polysaccharide synthesis in Klebsiella pneumoniae Chedid (O1:K2) was investigated. Deletion analyses and Southern hybridization studies suggested that the central region of the cloned 29-kb BamHI fragment is indispensable for K2 capsular polysaccharide synthesis. The 24,329-bp nucleotide sequence of the Klebsiella cps region was determined and deposited in the EMBL and GenBank databases through DDBJ and assigned accession number D21242. Nineteen possible open reading frames (ORFs) were identified in the sequenced area. Among them, 13 ORFs are very close to each other. Six of the 19 ORFs show considerable nucleotide sequence similarities to Salmonella typhimurium cpsG, cpsB, rfbP, and orf2.8, Escherichia coli gnd, and Haemophilus influenzae bexD, respectively. Moreover, the deduced amino acid sequence of the ORF10 product demonstrated a highly hydrophobic profile and showed putative membrane topology ...

Anti-polysaccharide immunity is a key facet of protection against several bacterial pathogens. Problems exist with current polysaccharide vaccines and alternative strategies that deliver a protective response are needed. We have identified immunological peptide mimics of type 6B and 9V pneumococcal capsular polysaccharides that could be used as vaccine antigens. Peptides mimicking antigenic properties of serotype 6B capsular polysaccharide were obtained from a phage-displayed peptide library expressing dodecameric peptides, using a human monoclonal antibody (Db3G9). A murine monoclonal antibody (206, F-5) against the serotype 9V capsular polysaccharide identified three peptide mimotopes from the dodecameric peptide library and one from a random pentadecameric peptide library. In ELISA, binding of 206, F-5 and Db3G9 to phage displaying the selected mimotopes was significantly inhibited by type-specific pneumococcal polysaccharide. Peptides were conjugated to keyhole limpet haemocyanin and were ...

Regulation of chain length is essential to the proper functioning of prokaryotic and eukaryotic polysaccharides. Modulation of polymer size by substrate concentration is an attractive but unexplored control mechanism that has been suggested for many polysaccharides. The Streptococcus pneumoniae capsular polysaccharide is essential for virulence, and regulation of its size is critical for survival in different host environments. Synthesis of the type 3 capsule [-4)-β-d-Glc-(1-3)-β-d-GlcUA-(1-] from UDP-glucose (UDP-Glc) and UDP-glucuronic acid (UDP-GlcUA) is catalysed by the type 3 synthase, a processive β-glycosyltransferase, and requires a UDP-Glc dehydrogenase for conversion of UDP-Glc to UDP-GlcUA. Strains containing mutant UDP-Glc dehydrogenases exhibited reduced levels of UDP-GlcUA, along with reductions in total capsule amount and polymer chain length. In both the parent and mutant strains, UDP-Glc levels far exceeded UDP-GlcUA levels, which were very low to undetectable in the absence ...

The role of the polysaccharide capsule is well established in UPEC virulence. The prototypical group 2 capsule provides protection against phagocytosis and complement-mediated killing (23, 24), contributes to immune evasion via molecular mimicry (33), and can also mask other surface-associated antigens (39). Group 2 capsule biosynthesis is controlled by a conserved export and assembly mechanism (16, 17, 19-22), suggesting that the expression of all group 2 capsules may be regulated by the same factors, hence providing an attractive target for the development of therapeutic agents.. We first examined a collection of 126 complete E. coli genomes to gain a better understanding of the distribution of the group 2 capsule types. We found that the majority of phylogroup B2 (75%; 33/44) and D (75%; 3/4) strains possess intact region I and III capsular export and assembly genes but carry an array of region II genes that encode different capsular K types. All 10 of the ST95 strains in our 126-strain ...

pneumococcus capsule, micrograph of pneumococcus, Gram-stain of pneumococci in sputum, bacterial capsule in virulent streptococci, encapsulated streptococcus pneumoniae

Analysis of Campylobacter jejuni capsular loci reveals multiple mechanisms for the generation of structural diversity and the ability to form complex heptoses: Mol.Microbiol.

Abstract Background and objectives: important virulence factor for many invasive bacterial pathogens of humans. Escherichia coli offer a model system to study the mechanisms by which capsular polysaccharides are synthesized and exported onto the cell surface of bacteria. Biosynthesis of the E consists of the repeat structure -4) GlcA- (1, 4)-GlcNAc- (1-, ...

Bacterioides fragilis is a gram-negative anaerobe and a prototypic gut commensal organism. B. fragilis is able to produce eight different capsular polysaccharides, which envelope the organism and form its protective capsule. Polysaccharide A (PSA) is one of these polysaccharides and, interestingly, has a unique zwitterionic motif that includes alternating positively and negatively charged sugar residues. PSA consists of a tetrasaccharide repeat unit that forms a polymer of between 30-300 repeats.. Such bacterial capsular polysaccharides are well known to industry, and are the basis of polysaccharide conjugate vaccines used successfully for decades in millions of people. The specific zwitterionic polysaccharide PSA was shown, in a series of landmark papers by our companys scientific founders that overturned established immunologic paradigms, to be processed via the endosomal pathway in antigen-presenting cells, depolymerized by NO-dependent deamination, and presented to CD4+ T-cells by MHC class ...

Cell Wall and Capsule,Capsular and extracellular polysacchrides,Serotype determining Capsular polysaccharide biosynthesis in Staphylococcus,Capsular polysaccharide synthesis enzyme ...

Cell Wall and CapsuleCapsular and extracellular polysacchridesSerotype determining Capsular polysaccharide biosynthesis in Staphylococcus Capsular polysaccharide synthesis enzyme Cap5K ...

Pills / Capsules are the easiest way to take most herbal formulas. There is a difference in pills and capsules when formulas are made in traditional manners.. Capsules are powdered herbal formulas and capsuled in gelatin. Our herbal capsules are 750mg,not the typical 500mg. Thats 25,000 milligrams more that competitors in 100 capsules!. All of our capsule formulas include 20-50% extracts for optimal performance. This also raises our minimum herbal amount to 1250mg per capsule, over double what your getting elsewhere. There are no extra strength formulas here because all of pills and capsules are already extra strength!. ...

Pills / Capsules are the easiest way to take most herbal formulas. There is a difference in pills and capsules when formulas are made in traditional manners.. Capsules are powdered herbal formulas and capsuled in gelatin. Our herbal capsules are 750mg,not the typical 500mg. Thats 25,000 milligrams more that competitors in 100 capsules!. All of our capsule formulas include 20-50% extracts for optimal performance. This also raises our minimum herbal amount to 1250mg per capsule, over double what your getting elsewhere. There are no extra strength formulas here because all of pills and capsules are already extra strength!. ...

Group II K antigens such as the K5 are associated with Escherichia coli causing serious extraintestinal infections. Genes for the production of group II capsules (kps) are organised into three functional regions 1, 2 and 3. The central region 2 encodes proteins involved in the biosynthesis of type specific polysaccharide. Proteins encoded by the flanking regions 1 and 3 are involved in the export of polysaccharide across the bacterial membranes unto the cell surface. Translocation of polysaccharide across the inner membrane is mediated by two region 3 encoded proteins, KpsM and KpsT which belong to the ATP-binding cassette (ABC) superfamily of transporters. Region 1 encodes six proteins, KpsF-E-D-U-C-S. The KpsE protein was shown to localise in the inner membrane and mutants lacking the encoded protein were unable to export polysaccharide to the cell surface. To understand the export role of the KpsE, its membrane topology was determined using TnphoA mutagenesis and beta-lactamase fusions. The ...

Carlos M Suligoy, Rocío E Díaz, Ana-Katharina Gehrke, Natalie Ring, Gonzalo Yebra, Joana Alves, Marisa I Gómez, Sindy Wendler, J Ross Fitzgerald, Lorena Tuchscherr, Bettina Löffler, Daniel O Sordelli, Mariángeles Noto Llana, Fernanda R Buzzola ...

According to this study, there was a significant difference in the risk of complications after lipoma resection depending on the presence of a capsule. Firstly, the authors concluded that in an encapsulated lipoma, the margins with the surrounding soft tissue are clear; therefore, capsule-containing resection is possible. However, in a nonencapsulated lipoma, it is difficult to define the plane of resection, which can increase the likelihood of delayed wound healing.. Secondly, nonencapsulated lipomas are more likely than encapsulated lipomas to develop in a dependent position. In this study, nine nonencapsulated lipomas occurred in the posterior aspect of the neck and 12 nonencapsulated lipomas occurred in the back, which was significantly higher than the incidence of encapsulated lipomas in those areas. If patients spend a long time lying down, with continuous irregular application of pressure over the site, the resultant poor circulation could aggravate the wound healing process.. Instead of ...

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Petri dish culture of a yeastlike organism (fungus), Rhodotorula sp, known as a red yeast to the production of a carotenoid pigment. This Fungus is a saprophytic organism, meaning that it acquires nutrition from dead or decaying tissue, either plant or animal. As a food spoilage organism it can contaminate domestic & industrial food. Rhodotorula sp appears in two forms, capsulated & noncapsulated. The noncapsulated variety is seen here. The capsulated version produces a slimy mucilage, the surface becoming smooth & shiny. Its function is uncertain, but in pathogenic species it is associated with increased virulence. - Stock Image B250/0197

Acid-resistant capsules are designed to be insoluble at low pH in order to allow the capsule to pass the stomach before dissolving in the intestines. Acid-resistant capsules manufactured by Dah Feng are made from HPMC and Pectin and are very suitable for probiotics, enzymes and other acid sensitive ingredients.. The capsules are available in sizes #1, #0 and #00.. ...

Product Description: Human monoclonal antibody against pneumococcal capsular polysaccharide Serotype 2Catalog Number: M1001 Quantity: • Research: 250 µg/mL, 200 µL, $450o Availability: ships within 2-3 weeks• Standard*: 1 mg/mL, 1 mL, $3,000o Availability: made to order, ships within 8-12 weeks• Custom: contact Micaela

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Author: Allen Loyd V Jr, Year: 1999, Abstract: The capsule is one of the most versatile of all dosage forms. In this article, the author discusses historical considerations, contemporary use, applications/uses, preparation of capsules (filling liquids in hard-gelatin capsules, filling capsules with a semisolid mass, filling solids in hard-gelatin capsules, preparing altered-release capsules, preparing coated capsules, beaker-flask coating, dipping and spraying and cleaning and packaging extemporaneously compounded capsules), quality control

Enzyme immunoassay for the determination of IgG antibodies to Pneumococcal Capsular Polysaccharide (PCP) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

Enzyme immunoassay for the determination of IgG antibodies to Pneumococcal Capsular Polysaccharide (PCP) in human serum or plasma. SmartEIA kit is specifically designed for automated analysis using the Agility instrument.

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TY - JOUR. T1 - Novel rkp gene clusters of Sinorhizobium meliloti involved in capsular polysaccharide production and invasion of the symbiotic nodule. T2 - The rkpK gene encodes a UDP-glucose dehydrogenase. AU - Kereszt, Attila. AU - Kiss, Erno. AU - Reuhs, Bradley L.. AU - Carlson, Russell W.. AU - Kondorosi, Ádám. AU - Putnoky, Péter. PY - 1998/10. Y1 - 1998/10. N2 - The production of exopolysaccharide (EPS) was shown to be required for the infection process by rhizobia that induce the formation of indeterminate nodules on the roots of leguminous host plants. In Sinorhizobium meliloti (also known as Rhizobium meliloti) Rm41, a capsular polysaccharide (KPS) analogous to the group II K antigens of Escherichia coli can replace EPS during symbiotic nodule development and serve as an attachment site for the strain-specific bacteriophage φ16-3. The rkpA to -J genes in the chromosomal rkp-1 region code for proteins that are involved in the synthesis, modification, and transfer of an ...

Child;Meningitis, Haemophilus;Haemophilus influenzae type b;Immunoglobulin Km Allotypes;Haemophilus Vaccines;Immunization;Vaccination;Bacterial Capsules;Pertussis Vaccine;Immunoglobulin Gm Allotypes;Meningitis;Antibody ...

Host defense mechanisms can counteract the antiphagocytic bacterial capsule. Unlike classical complement pathway, the alternative complement pathway is not antibody-mediated and can easily overwhelm encapsulated bacteria. It is triggered by the cleavage of C3 into C3a and C3b and the attachment of C3b to MAMPs (Microbe Associated Molecular Patterns), such as the capsular polysaccharides. The C3b molecules osponsize the pathogen, facilitating its phagocytosis and intravascular clearance. Furthermore, the complement cascade leads to the formation of proteolytic C3and C5 convertases and the terminal membrane attack complex, which lyses the pathogen. However, a potentially fatal drawback from lysing the pathogen is the release of endotoxic lipopolysaccharides, which elicit an out-of-control immune response. [2 ...

The invention described herein provides human antibodies produced in non-human animals that specifically bind to Streptococcus pneumoniae capsular polysaccharide (PPS-3). The invention further provides methods for making the antibodies in a non-human animal and for expressing the antibodies in cells including hybridomas and recombinant host cell systems. Kits and pharmaceutical compositions comprising the antibodies are also provided in addition to methods of treating, inhibitng or preventing S. pneumoniae infection or conditions or disorders caused by such infection by administering to a patient the pharmaceutical compositions described herein.

van Alphen, Loek, Caugant, Dominique A, Duim, Birgitta, ORourke, Maria and Bowler, Lucas (1997) Differences in genetic diversity of noncapsulated Haemophilus influenzae from various diseases. Microbiology, 143 (4). pp. 1423-1431. Full text not available from this repository ...

Pathogenic meningococci have obtained a 24?kb capsule synthesis isle (and alleles in meningococcal isolates was retained for everyone serogroups except serogroup Electronic that includes a synthetic requirement of UDP-GalNAc. inhabitants2,3 but invades the web host from time to time, leading to meningitis4 and septicaemia. In both and connections with host cellular material are modulated with the glycome in the bacterial surface area which include the lipooligosaccharide (LOS) as well as the glycosylation position of the sort IV pilin5. Meningococci are recognized from gonococci by the current presence of a capsule polysaccharide synthesis (isle includes a lower G/C-content compared to the primary meningococcal genome in keeping with acquisition via horizontally gene TMC353121 IC50 transfer (HGT) by recombination6. The overall organisation from the isle is as comes after: Area A is in charge of the formation of the capsule polymer; Area B (and which really is a homologue of the transcription ...

Sigma-Aldrich offers abstracts and full-text articles by [Han Zheng, Shaobo Ji, Zhijie Liu, Ruiting Lan, Ying Huang, Xuemei Bai, Marcelo Gottschalk, Jianguo Xu].

Streptococcus pneumoniae type 14兔多克隆抗体(ab79528)经Agg实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。

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During a national surveillance program on Group B streptococci (GBS) maternal carriage and neonatal infections, a GBS strain isolated from a pregnant womans vagino-rectal swab was non typable by either serological or molecular methods. Further molecular characterization demonstrated that the strain …

Bacteria evolving resistance against the action of multiple drugs and its ability to disseminate the multidrug resistance trait(s) across various strains of the same bacteria or different bacterial species impose serious threat to public health. Evolution of such multidrug resistance is due to the fact that, most of the antibiotics target bacterial survival mechanisms which exert selective pressure on the bacteria and aids it to escape from the action of antibiotics. Nonetheless, targeting bacterial virulence strategies such as bacterial surface associated polysaccharides biosynthesis and their surface accumulation mechanisms may be an attractive strategy, as they impose less selective pressure on the bacteria. Capsular polysaccharide (CPS) or K antigen that is located on the bacterial surface armors bacteria from host immune response. Thus, unencapsulating bacteria would be a good strategy for drug design, besides CPS itself being a good vaccine target, by interfering with CPS biosynthesis and surface

The genes (cps) involved in the synthesis of the colanic acid capsular polysaccharide in Escherichia coli K-12 are transcriptionally regulated by numerous proteins. Two of these, RcsB and RcsC, share homology with two-component regulatory elements that respond to environmental stimuli. Osmotic shock by sucrose or NaCl transiently increased transcription of a cpsB::lacZ fusion. RcsC and RcsB were essential for osmotic induction of colanic acid synthesis. In contrast to observations in some other osmotically regulated systems, addition of glycine betaine enhanced the osmotic induction of cps::lacZ by both sucrose and NaCl but had no effect alone. ...

Abs to a number of polysaccharide Ags have several common attributes: CDR-H3 is implicated in binding to Ag (28, 29), its length is strictly maintained (20, 30), and its sequence is almost invariably characterized by the presence of hydrophilic tyrosine residues (21, 31, 32). Xu and Davis (9) showed that, in VH-restricted mice, CDR-H3 diversity was sufficient for the development of specific Ab responses to a variety of hapten and protein Ags but not for two bacterial polysaccharide Ags. The latter finding was attributed to the failure of the single VH gene in their mouse model to accommodate the polysaccharide-specific response, which suggested VH dependency. Work by Nakouzi and Casadevall (33) showed that, in addition to CDR-H3, CDR-H2 encoded amino acids critical for the generation of Abs specific for the polysaccharide galactoxylomannan. These studies highlighted the significant roles that both the VH, as a whole, and the CDR component of the VH, as a part, can play in the generation of ...

Autor: Hicke, H. G. et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 1999; Keywords: biotechnology|br/|membrane reactors|br/|membrane-bound enzymes|br/|microporous and porous membranes|br/|polysaccharide synthesis|br/|photo-grafted membranes|br/|chromatography; Titel: Novel enzyme-membrane reactor for polysaccharide synthesis

Swisse Ultivites are available in capsule form. One Ultivite tablet is equivalent to two capsules so we recommend that you take 2 capsules daily.. Capsules are smaller in size so they do not fit as much of each ingredient in each capsule in comparison to each tablet. Capsules are easy to swallow so are an ideal option if you have difficulty swallowing tablets. Some tablets are known to take longer to break down (disintegrate) in the body than capsules, however, with Swisses advanced manufacturing technologies, both the Swisse Ultivite tablets and capsules are highly absorbable, and take approximately the same time, around 25 minutes, to break down in the digestive tract.. Capsules cost approximately 10% more due to the additional manufacturing costs and materials involved.. ...

Currently, Dr. Chen is seeking to decipher the molecular evolution of the emergence of CRE. CRE have been described as nightmare bacteria , and been classified as an urgent threat by CDC. CRE have increased over the past decade and grown resistance to even the most powerful antibiotics. The worldwide dissemination of CRE is predominately associated with a single K. pneumoniae clone, ST258. Using the whole genomic analysis, Dr. Chen and his colleagues have revealed that the ST258 is a hybrid strain that originated as a large chromosomal recombination. Meanwhile, this superclone is under significantly diversification, primarily due to the recombination of antigen encoded capsular polysaccharide synthesis (cps) region and plasmid acquisition. Thus far, they have sequenced over 300 CRE isolates, including the first two completely closed ST258 genomes; and have completely characterized over 50 carbapenem-resistant plasmids ...

The pharmaceutical industry segment by end-user is projected to attain a largest share of overall empty drug capsule market. In terms of geography, with 43% share in 2015, North America grabbed the largest market of empty drug capsule aided by U.S. Moreover, growing pharmaceutical industry in this region is predicted to foster the growth of empty drug capsule market. Apart from this, Europe empty drug capsule market accounted for USD 248.1 Million in 2015.. Request Free Sample Report @ https://www.researchnester.com/sample-request/2/rep-id-175. Expansion of Pharmaceutical and Nutraceutical Industries. Robust expansion of pharmaceutical and nutraceutical industries in developing and developed nation on the back of positive GDP figure is believed to be the dynamic factor behind the rapid growth of empty drug capsule market. Furthermore, growing adoption of empty drug capsule on the back of absence of odor and bitterness is also believed to flourish the growth of empty drug capsule market.. Rise in ...

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This is the current working model of the regulation. On the left we have the capsule being synthesised. The details are a little much for here but C is a protein embedded in the membrane, it interacts with D, a tyrosine kinase. A tyrosine kinase is an enzyme that adds a chemical group called a phosphate to something else. In this system it actually adds that group to itself and when it does it forces itself to change shape. This altered shape in D (represented on the right of the diagram) is thought to either change Cs shape as well or the change is simply relayed through C to the outside of the cell and production of capsule stops. Instead when D is phosphorylated any complete capsule is stuck to the cell wall. As this process is not a one way switch we need to return to the left of the diagram and this is achieved due to the activity of B which is able to remove phosphate groups from D ...

article{934d5e3b-3125-4a8e-9594-9c88d7695cb4, abstract = {Low-density polethylene disks with smooth or course surfaces were implanted in the abdominal wall of rats, and the tissue response was evaluated after 1, 6, or 12 weeks. Cell damage was detected by two different methods. Cells with increased membrane permeability could be identified using fluorescence microscopy by injection of propidium iodide prior to the killing of the rats. Second, cell death was verified by detection of DNA fragmentation. At 1 week a considerable number of the interfacial cells was stained with propidium iodide. Propidium-iodide-positive cells also were enriched at the edges of the disks irrespective of surface texture. The numbers of positive interfacial cells decreased markedly over time. Cells with DNA fragmentation initially displayed a scattered distribution; at later time points they appeared mainly in the outer portion of the enveloping capsule. The reactive capsule was thicker for the smooth surface, and ...

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All isolates harbored the cylE and hylB genes and at least one pilus island. Four (4.8%) of the 83 GBS isolates did not produce a hemolytic halo around the bacterial colonies (Figure 1). CP-868596 manufacturer Concomitantly, these isolates were not able to produce the orange carotenoid pigment in Granada medium. Most of the isolates harbored PI-2a alone (n = 30, 36.1%) or in combination with PI-1 (n = 42, 50.6%). PI-2a was distributed in all capsular types. identified in this study, including the type IX and NT isolates. However, the presence of this pilus island alone or in combination with PI-1 was found mainly in capsular type Ia and V, respectively. Besides, PI-1 was also found in combination with PI-2b (n = 4, 4.8%) and all isolates belonged to capsular type III. The presence of PI-2b alone was observed in seven isolates (8.4%) and all belonged to capsular type. Ia. All isolates grouped in MTs 1 (n = 16, 19.3%), 4 (n = 8, 9.6%), 6 (n = 5, 6.0%) and 7 (n = 7, 8.4%) harbored PI-1 and PI-2a ...

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Presence of less heavily encapsulated Klebsiella pneumoniae capsular type 1 in semen of healthy stallions and cervical swabs of mares suffering from metritis and comparison of virulence between heavily and less heavily encapsulated strains. （1988 ...

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The wireless motility-recording capsule, SmartPill, is an ingested one-time use electronic capsule that measures gastrointestinal luminal pressure, pH and temperature along the whole gastrointestinal tract. The pH profile and the pressure patterns define the time at which the capsule moves from the stomach to the duodenum and from the ileum to the caecum, whereas changes in temperature define the times of ingestion and expulsion. The recordings from the wireless motility capsule are sent from a radio transmitter in the capsule to a receiver carried around the waist. Wireless motility recordings have several advantages over imaging and manometry that have been used previously. Primarily the simplicity by which motility recordings are done, as well as the tolerability for the patient and limited need of assistance are valuable aspects. The wireless motility capsule provides standardized measures of transit with relevant normal values for the clinical work-up of patients with motility ...

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to describe what are the main functions of polysaccharides in plants, you need to know what is a polysaccharide. Polysaccharides are the carbohydrates.

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