Objective: Relevant animal models to study effects of bacterial aggregates on wound healing are lacking. We aimed at establishing an equine wound model with bacterial aggregates to investigate the impact of bacterial inoculation on normal (thorax) and impaired (limb) wound healing. Approach: Wounds were created on three limbs and both thorax sides of six horses. Twelve out of 20 wounds per horse were inoculated with 104 Staphylococcus aureus and 105 Pseudomonas aeruginosa on day 4. Healing was monitored until day 27 by clinical assessment, including wound scoring, surface pH measurements, and digital photography for area determination. Biopsies were used for bacterial culture and for peptide nucleic acid fluorescence in situ hybridization to detect bacterial aggregates. Results: Inoculated limb wounds healed slower than noninoculated limb wounds from day 10 onward ( p , 0.0001). Inoculated and noninoculated thorax wounds healed equally well and faster than limb wounds. The odds ratio of ...
Purpose: Bacterial adhesion and colonization play a crucial function in the pathogenesis of peri-implant tissue infection, which is considered the main cause of fixture loss. The aim of this study is to evaluate the differences in bacterial adhesion between a machined titanium surface, a double acid etched surface (Osseotite®) and an Osseotite surface with Nanometer-scale Discrete Crystalline Deposition (DCD™) of calcium phosphate (CaP)(Nanotite®).Methods: Surface roughness properties of each sample were determined by a laser profilometer and scanning electron microscopy (SEM) observation. Bacterial adhesion on machined, Osseotite®, and Nanotite® discs were performed using the following bacterial strains: Streptococcus mutans CCUG 35176, Streptococcus sanguis CCUG 17826, Streptococcus salivarius CCUG 11878, Actinobacillus actinomycetecomitans CCUG 37002, Porphyromonas gingivalis CCUG 2521. The assessment of bacterial adhesion was performed by comparing two methods: Total Viable Count (TVC) ...
Many bacteria, both environmental and pathogenic, exhibit the property of autoaggregation. In autoaggregation (sometimes also called autoagglutination or flocculation), bacteria of the same type form multicellular clumps that eventually settle at the bottom of culture tubes. Autoaggregation is generally mediated by self-recognising surface structures, such as proteins and exopolysaccharides, which we term collectively as autoagglutinins. Although a widespread phenomenon, in most cases the function of autoaggregation is poorly understood, though there is evidence to show that aggregating bacteria are protected from environmental stresses or host responses. Autoaggregation is also often among the first steps in forming biofilms. Here, we review the current knowledge on autoaggregation, the role of autoaggregation in biofilm formation and pathogenesis, and molecular mechanisms leading to aggregation using specific examples ...
The adhesin involved in diffuse adherence (AIDA) is an autotransporter protein that confers the diffuse adherence phenotype to certain diarrheagenic Escherichia coli strains. It consists of a 49 amino acid signal peptide, a 797 amino acid passenger domain, and a 440 amino acid beta-domain integrated into the outer membrane. The beta-domain consists of two parts: the beta(1)-domain, which is predicted to form two beta-strands on the bacterial cell surface, and the beta(2)-domain, which constitutes the transmembrane domain. We have previously shown that the beta-domain can be folded from the urea-denatured state when bound to a nickel column during purification. It has not been possible to achieve proper refolding of the beta-domain in solution; instead, a misfolded state C is formed. Here, we characterize this misfolded state in greater detail, showing that despite being misfolded, C can be analyzed as a conventional conformational state, with cooperative unfolding in urea and SDS as well as ...
Structural studies of biological macromolecular assemblies are providing an understanding of cellular function. In our laboratory, we utilize electron microscopy and image reconstruction to investigate questions about how adhesion pili aid pathogenic bacterial survival under harsh physiological conditions. Work in the lab currently focuses on bacterial adhesion pili and poliovirus polymerase. These projects address basic medical research questions directed at understanding bacterial adhesion to human host tissue and viral replication. Our data support development of novel therapeutics targeting these important health issues including, for example, development of a vaccine against travelers diarrhea ...
PhD Project - Molecular mechanisms modulating host epithelial integrity in response to bacterial adhesion at University of Birmingham, listed on FindAPhD.com
Working with a pathogenic strain of E. coli, the researchers found that the bacteria can sense attachment to the human intestinal cells and activate gene expression in response. This was demonstrated by engineering one of these genes to express a protein that stains the expressing bacteria to appear green under the microscope. Under microscopic examination, the researchers observed that only the attached bacteria fluoresce in bright green, whereas non-attached bacteria remain dark ...
Bacterial resistance to antibiotics is increasing at a high rate in both developing and developed countries. To circumvent the problem of drug-resistant bacterial pathogens, we need to develop new effective methods, substances, and materials that can disarm and prevent them from causing infections. However, to do this we first need to find new possible targets in bacteria to approach and novel strategies to apply.Escherichia coli (E. coli) bacteria is a normal member of the intestinal microflora of humans and mammals, but frequently cause diverse intestinal and external diseases by means of virulence factors, which leads to hundreds of million sick people each year with a high mortality rate. An E. coli bacterial infection starts with adhesion to a host cell using cell surface expressed adhesion polymers, called adhesion pili. Depending on the local environment different types of pili are expressed by the bacteria. For example, bacteria found in the gastrointestinal tract commonly express ...
Mannose, Apoptosis, Bacteria, Bacterial Adhesion, Bladder, Cell, Cells, Clathrin, Colitis, Contract, Crohns Disease, Cytoplasm, Diabetes Mellitus, Disease, E Coli, Endoplasmic Reticulum, Epithelial Cell, Epithelial Cells, Escherichia, Escherichia Coli
Fang B, Gon S, Park M, Kumar K-N, Rotello VM, Nusslein K, Santore MM. 2011. Bacterial adhesion on hybrid cationic nanoparticle-polymer brush surfaces: ionic strength tunes capture from monovalent to multivalent binding.. Colloids Surf B Biointerfaces. 87(1):109-15. ...
Biofilms are ubiquitous in aquatic environments. Biofilms have been shown to attract and harbor pathogens such as P. aeruginosa and Legionella pneumophila in premise plumbing system. The fact that biofilms can protect attached bacterial cells from disinfectants raises rudimentary questions regarding interactions of bacterial cells with biofilm surfaces. Consequently, the main objectives of this study were to: 1) investigate the mechanisms that govern E. coli S17, E. coli 14f and Legionella cells adhesion on clean PVC, copper and biofilms; 2) examine the role of disinfectants on biofilms structure and subsequent effect on bacterial adhesion. Mechanisms of three strains of bacteria attachment on biofilms grown on PVC and copper surfaces were investigated. Biofilms were grown in CDC reactors using different types of feed water such as groundwater, monochloramine-treated groundwater, dechlorinated tap water and tap water. Biofilm physical structure was characterized at micro- and meso-scales using ...
The global issue of nosocomial infection is owing to bacterial colonization and biofilm formation on medical devices which primarily affects critically ill and/or immuno-compromised patients and also leads to malfunctioning of the devices. Therefore, it is desirable to prevent bacterial colonization on these devices by coating with a non toxic antimicrobial agent or bacterial adherence inhibitor. Here we have shown Bacillus licheniformis JS2 derived selenium nanoparticles (SeNPs) inhibit Staphylococcus aureus adherence and micro-colony formation on polystyrene, glass, and catheter surface. Results indicated that, the coating of these non toxic biogenic SeNPs, at a concentration of 0.5mgSe/ml, prohibits bacterial load to more than 60% on glass and catheter surface, when incubated at 4°C for 24h in phosphate buffered saline. Furthermore, confocal and electron microscopic observations strongly suggested the inhibition of biofilm and micro-colony formation on SeNP coated glass and catheter surfaces ...
Adherence ability of Staphylococcus epidermidis on prosthetic biomaterials: an in vitro study [Corrigendum] Â Shida T, Koseki H, Yoda I, et al. Int J Nanomed. 2013;8(1):3955â 3961.On page 3955, please note that the first affiliation has changed from Department of Orthopedic Surgery, Graduate School of Medicine, Nagasaki University, Nagasaki, Japan to Department of Orthopedic Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.Read the original article
Ileal lesions in Crohns disease (CD) patients are colonized by pathogenic adherent-invasive Escherichia coli (AIEC) able to adhere to and invade intestinal epithelial cells (IEC), and to survive within macrophages. The interaction of AIEC with IEC depends on bacterial factors mainly type 1 pili, flagella, and outer membrane proteins. In humans, proteases can act as host defence mechanisms to counteract bacterial colonization. The protease meprin, composed of multimeric complexes of the two subunits alpha and beta, is abundantly expressed in IECs. Decreased levels of this protease correlate with the severity of the inflammation in patients with inflammatory bowel disease. The aim of the present study was to analyze the ability of meprin to modulate the interaction of AIEC with IECs. In patients with ileal CD we observed decreased levels of meprins, in particular that of meprin β. Dose-dependent inhibition of the abilities of AIEC strain LF82 to adhere to and invade intestinal epithelial T84 cells was
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Pappelbaum, Karin I.; Gorzelanny, Christian; Graessle, Sandra; Suckau, Jan; Laschke, Matthias W.; Bischoff, Markus; Bauer, Corinne; Schorpp-Kistner, Marina; Weidenmaier, Christopher; Schneppenheim, Reinhard; Obser, Tobias; Sinha, Bhanu; Schneider, Stefan W. ...
Bacterial biofilms are sessile microbial communities that cause serious problems, such as antibiotic resistant chronic infections in humans, and persistent biofouling of engineering facilities. Biofilm formation is initiated by bacterial adhesion to a surface followed by the formation of microcolonies and further development of heterogeneous structures with water channels between cell clusters. The mechanism of biofilm structural heterogeneity and the bacterial genes involve in structural organization are still poorly understood. Nevertheless, once microbes adhere to a surface and form biofilm on it, they are up to 10-1,000 times more resistant to antimicrobial agents than their free-swimming counterparts. It is well accepted that biofilm formation involves multicellular behaviors, associated with major changes in microbial gene expression and protein synthesis. These changes are influenced by many environmental factors such as surface hydrophobicity, topography, chemistry, and charge. To better
Structural studies of biological macromolecular assemblies are providing an understanding of cellular function. In our laboratory, we utilize electron microscopy and image reconstruction to investigate questions about microbial virulence.. Work in the lab currently focuses on bacterial adhesion pili (fimbriae), type III secretion system needles, and virus replication. These projects address basic medical research questions directed at understanding bacterial adhesion to human host tissue and viral replication. Our data support development of novel therapeutics targeting these important health issues including, for example, development of a vaccine against travelers diarrhea.. ...
In this paired case-control study of infants with diarrhea in São Paulo, we examined the association between HEp-2-adherent Escherichia coli strains and diarrhea. We tested isolates from stool specimens of infants with diarrhea and matched controls in an HEp-2 cell adherence assay; we then hybridized isolates with DNA probes and identified enteropathogenic E. coli (EPEC), enteroaggregative E. coli (EAEC), and diffusely adherent E. coli (DAEC). From 100 patient-control pairs, we isolated 78 HEp-2-adherent strains; of these, 61 strains were single pathogens identified in stools of infants with diarrhea. While typical EPEC was significantly associated with diarrhea ( ...
Stinson, Murray W. and Jen Ren Wang (1997) Lectin inhibition of bacterial adhesion to animal cells. [Publication] Full text not available from this repository ...
BioFilm Pharma and BIOASTER (www.bioaster.org), the French Institute for Technological Research in Infectious Diseases and Microbiology, announced today the signature of a research contract.. To address the challenge of antibiotic resistance, this collaboration will lead to the deciphering of the mode of action of a new class of drugs.. This first project aims at identifying the mode of action of non-antibiotic anti-biofilms molecules through an integrated multi-omic analysis.. Anti-biofilm candidates of the study have already shown very promising results thanks to the BioFilm Ring Test® technology patented by the BioFilm Technologies group. As of today, this key technology is the only reliable and standardized in vitro biofilm method focusing on bacterial adhesion. Find more information on BIOASTER Press Release here.. ...
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Much work on bacterial adhesion has focussed on the colonisation of surfaces and the removal of mature biofilms. Little attention has been devoted to interactions within mature biofilms and how these might be manipulated in the cause of novel therapies. Calcium binding to oral streptococci displays …
The mechanisms by which bacteria adhere to inert surfaces are not well understood. The aim of this work was to elucidate the structural and genetic changes induced in a classical E. coli K-12 strain by a mutation allowing surface colonization. Electron microscopy of negatively stained bacteria revealed the presence of thin fibrillar pili at the surfaces of the mutant cells (Fig. 6). These particular pili seemed to be identical to the Congo red-binding structures described by Olsén and coworkers (22) as curli. When grown on CFA-Congo red indicator plates, the mutant strains were more intensely stained, indicating curli overproduction. The curli subunits are encoded by the csgA gene (21). Insertion of a reporter cassette into this gene revealed a 3.5-fold-higher transcription in the presence of the mutation responsible for the adherent phenotype. Furthermore, the introduction of a csgAnull mutation totally suppressed the biofilm-forming properties (Fig.2), demonstrating that curli production is ...
casSAR Dugability of Q2FUY2 | clfB | Clumping factor B - Also known as CLFB_STAA8, clfB. Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Partly responsible for mediating bacterial attachment to the highly keratinized squamous epithelial cells from the nasal cavity via an interaction with cytokeratin K10 (K10). Also promotes bacterial attachment to cultured keratinocytes, possibly through an interaction with cytokeratin K10. Binds mouse cytokeratin K10. Activates human platelet aggregation.
Microtiter plate-based bacterial biofilm assay is frequently used to study bacterial biofilm development and growth. While this assay is simple and relatively high-throughput, it frequently shows difficulty in establishing robust biofilm attachment in the wells. We report that the consistency of bacterial biofilm a
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es found that the beads and the bacterial aggregates collided up to a ... The Penn State engineer explains that the fact that large aggregates...Now using the new approach developed by Logan researchers not only ...In addition in studying the ocean processes Logan has identified a ......,Faster,coagulation,rates,found,in,natural,systems,could,impact,industrial,processing,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
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Spi-Ceramic coatings are highly adherent nanocrystalline thin films deposited using N2 Biomedicals low temperature ion beam assisted deposition process. Al
Methods for treating patients in which damaged tissue or an indwelling prosthetic device or catheter has a bacterial biofilm growing thereon, to at least partially disrupt said biofilm, by administering at least one antibacterial enzyme that is lethal or damaging to the biofilm-forming bacteria in an amount that is effective to at least partially disrupt the biofilm upon contact therewith. Methods for prophylactically treating a patient, and methods for disinfecting or sterilizing a surface ex-vivo to remove a biofilm or prevent biofilm growth are also disclosed, as well as implantable articles susceptible to biofilm growth to which a prophylactic coating of an antibacterial enzyme has been applied.
How to treat HEK293 with S9 fraction? - posted in Tissue and Cell Culture: Hi, everyone In my experiment, i need to treat HEK293 cells with drug including S9 fraction. In the protocol of S9. The medium including S9 have to remove and wash with PBS after treating in 4 hours. But the adhesion ability of HEK293 cells are very week. The cells will be lost if i wash them. What can i do to improve it? Thanks a lot.
W]hile many behavioral traits have a heritable component, its not anything like what the naive extremists among the cognitive science crowd think. There are no genes that specify what you will name your dog [WTF? -ed]- in fact, most of the genes associated with the brain have very wide patterns of expression and functions that are not neatly tied to behaviors: how does an allele of an adhesion factor map to your performance on a math test? It doesnt, not directly. ...
Viral surface protein implicated in the binding to specific host surface molecule(s). This binding can lead to virion entry into the host cell, it can trigger signaling pathways, or it can allow the virion to be carried by the host cell to a specific organ ...
A community-based life style is the normal mode of growth and survival for many bacterial species. These cellular accretions or biofilms are initiated upon reco
whats a Eucaryotic cell?http://library.thinkquest.org/C004535/eukaryotic_cells.html. thanx, you guys are awesome!!. Any time!. eucaryote= no nucleu...
Methicillin resistant Staphylococcus aureus adhesion to human umbilical vein endothelial cells demonstrates wall shear stress dependent ...
The O26 serogroup of enteropathogenic Escherichia coli (EPEC) is one of the serogroups most frequently implicated in infant diarrhea and is also common among enterohemorrhagic E. coli (EHEC) strains. the most common O26 strains belong to EPEC/EHEC serotype O26:H11 and are generally Shiga toxin (Stx) positive. Stx-negative E. coli strains that are negative for the EPEC EAF plasmid and bundle-forming pilus (Bfp) are classified as atypical EPEC. Here, we report a novel adhesin present in an stx-negative bfpA-negative atypical EPEC O26:H11 strain isolated from an infant with diarrhea. A cloned 15-kb genomic region from this strain, designated the locus for diffuse adherence (lda), confers diffuse adherence on HEp-2 cells when expressed in E. coli K-12. Sequence analysis of lda revealed a G+C content of 46.8% and 15 open reading frames sharing homology with the E. coli K88 fae and CS31A clp fimbrial operons. the lda region is part of a putative 26-kb genomic island inserted into the proP gene of the ...
Enteropathogenic Escherichia coli (EPEC) strains possess genes for attaching and effacing (eae) and EPEC adherence factor (EAF) plasmid. It is necessary to develop molecular techniques for the evaluation of EPEC isolates. A total of 183 E. coli isolates from neonates admitted to Pusan National University Hospital were investigated by polymerase chain reaction (PCR) and DNA colony hybridization. Of the 183 isolates tested, 10 (5.5%) were positive for eae by PCR and DNA colony hybridization and confirmed to be EPEC. Ten EPEC isolates showed 3 different adherence patterns: seven strains had diffuse adherence, two localized adherence-like adherence, and one aggregative adherence. They were also examined by antimicrobial susceptibility tests, serotyping, and molecular epidemiological typing such as pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) analysis. The EPEC isolates could be divided into 9 different antimicrobial resistance patterns, 6 serotypes, 4 PFGE ...
Numerous studies have demonstrated the effects of laser-induced heat on demineralization of enamel; however, no studies have investigated the link between heat/laser-induced changes in physicochemical properties and bacterial adhesion. In this study, we investigated the effects of thermal treatment on surface properties of enamel such as hydrophobicity and zeta potential. Bacterial adhesion to treated surfaces was characterized by confocal laser scanning microscopy, and adhesion force was quantified by atomic force microscopy. The hydrophobicity of enamel increased after heating (p < 0.05), and the zeta potential of heated enamel became more negative than that of the control (p < 0.01). Streptococcus oralis and S. mitis were more hydrophilic than S. sanguis, with more negative zeta potential (all p < 0.01). S. mitis and S. oralis occupied significantly less area on enamel after being heated (p < 0.05). Heating reduced the adhesion force of both S. mitis and S. oralis to enamel with ...
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TY - JOUR. T1 - Quantification of Staphylococcus aureus cell surface adhesins using flow cytometry. AU - Mohamed, Nehal. AU - Visai, Livia. AU - Speziale, Pietro. AU - Ross, Julia M.. PY - 2000. Y1 - 2000. N2 - The initiation of many infectious diseases involves specific adhesion of bacteria to host tissue proteins and carbohydrates. Staphylococcus aureus is known to bind specifically to several proteins in the extracellular matrix (ECM). We report the quantification of the collagen and fibronectin adhesin densities on the staphylococcal surface using flow cytometry. Our results are in agreement with previous reports on the transcription of the respective genes and demonstrate different patterns of temporal expression for the two adhesins in the strains studied. We demonstrate a convenient technique for quantification of bacterial adhesins that can be used in studies aimed at characterization of bacterial adhesion to ECM components and understanding expression of adhesins during the course of an ...
Definition : Solutions designed to act as a temporary barrier inhibiting postsurgical adhesion between tissues and organs. These solutions are applied to the surface of tissues and organs at the end of surgery, before surgical closure, and are typically resorbed by the body in a short period (e.g., several days). Postsurgical adhesion inhibition solutions are intended for use in pelvic and gynecological surgery, both in open and laparoscopic procedures.. Related Terms : "Films, Postsurgical Adhesion Inhibition" , "Gel, Postsurgical Adhesion Inhibition". Entry Terms : "Surgical Adhesion Barrier Solutions" , "Adhesion Inhibition Solutions" , "Adhesion Barrier Solutions" , "Scarring Inhibitors" , "Postsurgical Scarring Inhibitors" , "Barriers, Adhesion, Resorbable". UMDC code : 20374 ...
Debate regarding the co-existence of Staphylococcus aureus and Pseudomonas aeruginosa in wounds remains contentious, with the dominant hypothesis describing a situation akin to niche partitioning, whereby both microorganisms are present but occupy distinct regions of the wound without interacting. In contrast, we hypothesised that these microorganisms do interact during early co-colonisation in a manner beneficial to both bacteria. We assessed competitive interaction between S. aureus and P. aeruginosa in biofilm cultured for 24-72 h and bacterial aggregates analogous to those observed in early (,24h) biofilm formation, and interaction with human keratinocytes. We observed that S. aureus predominated in biofilm and non-attached bacterial aggregates, acting as a pioneer for the attachment of P. aeruginosa. We report for the first time that S. aureus mediates a significant (P,0.05) increase in the attachment of P. aeruginosa to human keratinocytes, and that P. aeruginosa promotes an invasive ...
J. Saunier, J.M. Herry, C. Marliere, M. Renault, M.-N. Bellon-Fontaine, et al.. Modification of the bacterial adhesion of Staphylococcus aureus by antioxidant blooming on polyurethane films. Materials Science & Engineering. C, Materials for Biological Applications, 2015, 56, pp.522-531. 〈hal-01569038〉 ...
In natural environments, bacteria are often found as sessile communities known as biofilms (8, 10). To date, the bacterial structures of adherence (25, 40-42, 56) and the physiological processes involved in bacterial surface colonization (15, 40, 41) are better understood than the genetic responses of bacteria adhering to a surface. By using a library oflacZ fusions and a reliable screen for identifying genes whose expression changes in biofilm versus planktonic cells, the transcription of 38% of the E. coli genes was shown to be modified during the colonization process. Several genes with altered expression in biofilms were identified. Different cellular functions were induced in attached bacteria: the OmpC porin, the high-affinity transport system of glycine betaine, colanic acid production (theE. coli class I exopolysaccharide), tripeptidase T, and synthesis of a nickel high-affinity transport system. On the other hand, the syntheses of flagella and of a putative protein of 92 amino acids ...
Methods of inhibiting bacterial adhesion to medical implants and reducing device-associated infection are effectuated by administering an effective amount of apo-transferrin to an individual with such an implant. Preferably the apo-transferrin is administered by controlled release at or near the implant.
The Scientific World Journal is a peer-reviewed, Open Access journal that publishes original research, reviews, and clinical studies covering a wide range of subjects in science, technology, and medicine. The journal is divided into 81 subject areas.
Streptococcus pneumoniae (pneumococci) are Gram-positive cocci and commensals of the human upper respiratory tract. Pneumococcal pathogenesis requires adherence to host cells and dissemination through cellular barriers and to evade host defense mechanisms. The Pneumococcal surface protein C (PspC) is an important virulence factor which has a crucial role in pneumococcal adhesion to host cells and immune evasion by manipulating the host complement system. To elucidate the pneumococcal adherence and uptake mechanism via factor H glycosaminoglycans (dermatan sulfate and heparin) were employed as competitive inhibitors in infection experiments with epithelial cells or human polymorphonuclear leukocytes (PMNs). Glycosaminoglycans significantly inhibited the FH mediated pneumococcal adherence and subsequent invasion to host epithelial cells. Furthermore, the short consensus repeats of FH which promotes the adhesion of pneumococci to host cells were identified by blocking experiments with domain mapped
Streptococcus pneumoniae (pneumococci) are Gram-positive cocci and commensals of the human upper respiratory tract. Pneumococcal pathogenesis requires adherence to host cells and dissemination through cellular barriers and to evade host defense mechanisms. The Pneumococcal surface protein C (PspC) is an important virulence factor which has a crucial role in pneumococcal adhesion to host cells and immune evasion by manipulating the host complement system. To elucidate the pneumococcal adherence and uptake mechanism via factor H glycosaminoglycans (dermatan sulfate and heparin) were employed as competitive inhibitors in infection experiments with epithelial cells or human polymorphonuclear leukocytes (PMNs). Glycosaminoglycans significantly inhibited the FH mediated pneumococcal adherence and subsequent invasion to host epithelial cells. Furthermore, the short consensus repeats of FH which promotes the adhesion of pneumococci to host cells were identified by blocking experiments with domain mapped
Adherence of M. pneumoniae to a host cell (usually a respiratory tract cell, but occasionally an erythrocyte or urogenital lining cell) is the initiating event for pneumonic disease and related symptoms. The specialized attachment organelle is a polar, electron dense and elongated cell extension that facilitates motility and cytadherence to host cells. It is composed of a central filament surrounded by an intracytoplasmic space, along with a number of adhesins and structural and accessory proteins localized at the tip of the organelle. A variety of proteins are known to contribute to the formation and functionality of the attachment organelle, including the accessory proteins HMW1â "HMW5, P30, P56, and P90 that confer structure and adhesin support, and P1, P30 and P116 which are involved directly in attachment. This network of proteins participates not only in the initiation of attachment organelle formation and adhesion but also in motility. The P1 adhesin (trypsin-sensitive protein) is a 120 ...
Large externalized, repeat-rich proteins are emerging as important factors in the attachment of bacteria to biotic and abiotic surfaces. An intriguing new study of the plant-associated terrestrial microbe Pseudomonas putida by Manuel Espinosa-Urgels group that is reported in this issue of Molecular Microbiology has revealed that LapF, a huge protein (, 6000 aa) associated with the cell surface, is required for microcolony assembly from single attached cells, and in turn, formation of biofilms. Mutants defective in IapF exhibit competitive deficiencies in the rhizosphere. On both biotic and abiotic surfaces, these mutants undergo normal irreversible attachment, but cannot advance beyond this point to form multicellular clusters. The lapF phenotype is nutritionally conditional and is only manifested under a subset of growth regimes. Accordingly, lapF gene expression is controlled by the stress-responsive sigma factor RpoS and is elevated within growing microcolonies on abiotic surfaces and plant ...
Bacterial adhesins promote colonization at the initial stages of an infection by mediating attachment to host tissues, thus avoiding nonspecific host defenses such as mechanical clearance and allowing bacterial multiplication to occur within the host (1). To exert these functions, adhesins need to be presented at the surface of the bacterium. Like typical adhesins, B. pertussis FHA attaches the bacterium to receptors in the respiratory tract (17-21, 28). However, in addition to being surface-associated, large amounts of FHA are also released into the extracellular milieu (15). This has so far only been observed in vitro. In this work, we show for the first time that FHA is likely to also be released in vivo, and that its secretion is necessary for efficient colonization in a mouse model of infection. Our results support the paradigm that the secreted form of bacterial adhesins may participate in pathogenesis.. The use of B. pertussis strains deficient in FHA release but presenting FHA ...
Abstract Expression of the platelet-activating factor receptor is upregulated in the respiratory epithelium of smokers and chronic obstructive pulmonary disease patients. We have recently determined that increased expression of PAFr cor - relates with higher levels of adhesion to human bronchial epithelial cells by non-typable Haemophilus influenzae and Streptococcus pneumoniae which are major bacterial pathogens in acute exacerbations of COPD. In addition, we found that a PAFr antagonist decreased the adhesion of both respiratory bacterial pathogens to non-cigarette exposure con - trol levels. This highlights the possibility that epithelial receptors, that are upregulated in response to cigarette smoke, could be targeted to specifically block chronic bacterial infections of the lower respiratory tract. In this commentary, we explore the question of whether adhesion to a temporally-upregulated host receptor is a common event in chronic bacterial disease, and as such, could represent a putative ...
Biofilm Growth - Pseudomonas aeruginosa. Rocks in a stream bed are often slippery due to the growth of a mixed population of attached bacteria, algae and fungi. These Pseudomonas aeruginosa (bacteria) are shown growing on the surface of a fiber and forming a single species biofilm. - Stock Video Clip K003/2837
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Wounds are known to serve as portals of entry for group A Streptococcus (GAS). Subsequent tissue colonization is mediated by interactions between GAS surface proteins and host extracellular matrix components. We recently reported that the streptococcal collagen-like protein-1, Scl1, selectively binds the cellular form of fibronectin (cFn) and also contributes to GAS biofilm formation on abiotic su
Invasin and intimin are major virulence factors of enteropathogenic Yersiniae and Escherichia coli, mediating invasion into and intimate adherence to host cells, respectively. Several studies have hinted that extracellular portion of these homologous proteins might be exported via an autotransport mechanism, but rigorous experimental proof has been lacking. Here, we present a topology model for invasin and intimin, consistent with the hypothesis that the N-terminal β-barrel domain acts as a translocation pore to secrete the C-terminal passenger domain. We confirmed this topology model by inserting epitope tags into the loops of the β-barrel. We further show that obstructing the pore of β-barrel hinders the export of the passenger domain. As for classical autotransport, the biogenesis of invasin and intimin is dependent on the Bam complex and the periplasmic chaperone SurA, whereas the chaperone/protease DegP is involved in quality control. However, compared to classical autotransporters (Type Va
The aim of this in-vitro study was to investigate the influence of fillers in resin composites on the initial bacterial adhesion. For this investigation the strains of S. mutans and S. sanguinis ere used. Additionally, the influence of a pellicle, the inhibitor BHT and silane-treatment of the filler on the bacterial adhesion were examined. The adhesion of the two strains S. mutans and S. sanguinis was also compared. The 13 tested materials were experimental composites, based on the same monomer matrix. One of the composites was without fillers (Grundmasse). The other twelve composites showed a filler-weight fraction of 30%. Two different materials of fillers were used (SiO2, Ba-Al-B-Silikat) with different specific surfaces (0,6 to 150 m2/g). Two materials (K6 0% BHT, K6 1% BHT) differed in silane-treatment of the fillers (silane-treated or not silane-treated). For six composites (Ox 50, R709, DT4, GK 0,7 UF silane-treated, K6 silane-treated, K6 not silane-treated) there was a difference in the ...
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Facial mask pack for anti-wrinkle made by Bio-cellulose. Bio-cellulose is made by fermenting coconut juice with a microorganism, and it has excellent adherence ability and delivery ability for solutions. Also, since it has a cooling effect, it would be very good addtion for your catalog and your customers. Separetely from this, since we are handling... Learn More ...
Research groupsCell biology and Biotechnology Mechanisms of gene regulation and bacterial biofilm development Dr Fernando Govantes. ..
Biofilm formation - posted in Microbiology: Hello to forum members, I have 2 questions and your input is greatly appreciated. 1- What is the best way to grow bacterial biofilm on a plate and food? I am doing some reading on that and have not been able yet to tell the best way. Any one worked in biofilm??? Thx in advance!
Encounters with Epithelial Cells Over the past decade, many bacterial pathogens have been shown to enter epithelial cells (Fig. 114-2); the bacteria often use specialized surface structures that bind to receptors, with consequent internalization. However, the exact role and the importance of this process in infection and disease are not well defined for most of these pathogens. Bacterial entry into host epithelial cells is seen as a means for dissemination to adjacent or deeper tissues or as a route to sanctuary to avoid ingestion and killing by professional phagocytes. ...
Define adhesin: any of various specialized molecular components (such as proteins) on the surface of a bacterial cell that… - adhesin in a sentence
NBIC are hosting a third workshop, on Biofilm Management, after the success of previous workshops on Biofilm Detection and Biofilm Engineering, to explore challenges, opportunities and advances in Biofilm Management.
Neves, B. C., Knutton, S., Trabulsi, L. R., Sperandio, V., Kaper, J. B., Dougan, G., & Frankel, G. (1998). Molecular and ultrastrutural characterization of the EspA filaments from different EPEC serotypes. In Abstracts. Atlanta ...
Spontaneous bacterial colonization by CONS in Mgb-/- females versus Mgb+/- and WT controls.Bacteria recovered from urine (CFU/ml), bladders, and kidneys (CFU/or
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Looking for online definition of leukocyte adherence assay test in the Medical Dictionary? leukocyte adherence assay test explanation free. What is leukocyte adherence assay test? Meaning of leukocyte adherence assay test medical term. What does leukocyte adherence assay test mean?
Gregory Anderson, biology department at IUPUI, will present Molecular Insights into Bacterial Biofilm Formation in the Cystic Fibrosis Lung. Read more about Anderson at https://science.iupui.edu/people/anderson-gregory .
Human enterotoxigenic Escherichia coli adhere to the brush border of human enterocytes. The mean number of bacteria adhering to one enterocyte (adhesion index) varied from 0.5 to 3.1 when the strains produce adhesins. Different factors related to enterocytes and to bacteria are involved in this variability. The number of bacteria which adhered to enterocytes issued from the same donor varied from from 0 to 12. Moreover the proportion of enterocytes on which several bacteria sticked did not exceed 20%. This variability might be due to the disparity in the maturation of the enterocytes. On the other hand, whatever the adhesion factors considered, the adhesion index varied according to the donors. ETEC strains did not express adhesion when bacteria were grown in a liquid medium but this capacity could be restored after transfer on solid medium. This phenomenon seemed like a phase-variation and appeared to be linked to a 4 to 6 kilobases (kb) plasmid. On the other hand, when the bacteria were grown on agar
Certain EAEC strains have the potential to cause UTIs.Some of the urine isolates from hospital patients represent IPEC or at least they carry virulence-associated genes of diarrheagenic E. coli. Twenty-eight (10.9%) of the 265 urine isolates carried one or more known IPEC virulence genes, sometimes in combination with classical UPEC virulence genes. From these isolates, 23 carried the EAEC heat-stable enterotoxin 1-coding gene astA, and four of them clearly exhibited the typical EAEC aggregative adherence phenotype on both HEp-2 and T24 bladder epithelial cells. Although the function of this enterotoxin is not completely understood, it has been associated with diarrhea in children (47). Despite the fact that astA and, less frequently, the aap gene have been reported in E. coli pathotypes other than EAEC (39, 40, 48-52), their detection remains useful for the diagnosis of EAEC infection if they are screened in combination with other EAEC genes. However, the widespread presence of the astA gene in ...
Streptococcus pneumoniae is a major cause of morbidity and mortality worldwide. The ability of this bacterium to adhere to epithelial cells is considered as an essential early step in colonization and infection. By screening a whole genome phage display library with sera from infected patients, we previously identified three antigenic fragments matching open reading frame spr0075 of the strain R6 genome. This locus encodes for an 120-kDa protein, herein referred to as plasminogen- and fibronectin-binding protein B (PfbB), which displays an LPXTG cell wall anchoring motif and six repetitive domains. In this study, by using isogenic pfbB-deleted mutants of the encapsulated D39 and of the unencapsulated DP1004 type 2 pneumococcal strains, we show that PfbB is involved in S. pneumoniae adherence to various epithelial respiratory tract cell lines. Our data suggest that PfbB directly mediates bacterial adhesion, because fluorescent beads coated with the recombinant PfbB sp17 fragment (encompassing one ...
The authors have described a new model to directly study bacterial adherence to basement membrane using scanning electron microscopy 10. By using direct manual counting of surface adherent P. aeruginosa bacilli with scanning electron microscopy, they have determined the exact number of adherent bacteria on the collagen surface. This could be a more direct and specific, albeit more laborious, method to determine bacteria adherence than previous indirect assays ofbacterial adherence, such as radiolabelling techniques. Byrecent use of this model, the authors have shown that P. aeruginosa adherence to basement membrane collagen is reduced in the presence of low-dose erythromycin, probably partly due to alteration of bacterial morophology 10. These results showed that the lectin PHA-E, but not A. hypogea, significantly inhibited P. aeruginosa adherence to collagen. PHA-E appeared to inhibit P. aeruginosa adherence at 0.01, 0.1 and 1 mg·mL−1, although only the latter two concentrations inhibited ...
Pseudomonas aeruginosa is an extremely successful pathogen able to cause both acute and chronic infections in a range of hosts, utilizing a diverse arsenal of cell-associated and secreted virulence factors. A major cell-associated virulence factor, the Type IV pilus (T4P), is required for epithelial cell adherence and mediates a form of surface translocation termed twitching motility, which is necessary to establish a mature biofilm and actively expand these biofilms. P. aeruginosa twitching motility-mediated biofilm expansion is a coordinated, multicellular behaviour, allowing cells to rapidly colonize surfaces, including implanted medical devices. Although at least 44 proteins are known to be involved in the biogenesis, assembly and regulation of the T4P, with additional regulatory components and pathways implicated, it is unclear how these components and pathways interact to control these processes. In the current study, we used a global genomics-based random-mutagenesis technique, transposon ...
Flagella and flagellum-mediated motility are integral to the virulence of several gastrointestinal bacterial pathogens (10). For L. monocytogenes, no link has been made between flagella and virulence, although the flagella are important for efficient invasion of tissue culture cells (2, 6). In this study, we investigated the mechanism by which flagella influence the ability of L. monocytogenes to invade host cells and the role of flagella in colonizing mice early in infection. Our results clearly indicate that L. monocytogenes flagella do not function as adhesins to enhance bacterial attachment to and invasion of epithelial cells, but rather function as motility devices contributing more to invasion than a mere increase in probability of bacterium-host cell interaction. Moreover, we show that motile bacteria outcompete nonmotile bacteria for initial colonization of the intestinal tract and liver by L. monocytogenes.. Flagella can function as adhesins, independent of motility, to enhance ...
TY - PAT. T1 - Synthetic Disugar Hydrocarbons as Natural Analogs to Control Microbial Behaviors. AU - Wang,Guirong. AU - Luk,Yan-Yeung. PY - 2017/1/19. Y1 - 2017/1/19. N2 - Synthetic disaccharide hydrocarbons (DSHs) that reactive bacterials swarming motility and inhibit bacterial adhesion and biofilm formation. A library of DSHs were tested in several experiment for the impact on various Pseudomonas aeruginosa populations and compared against existing compounds to determine efficacy and utility. Certain DSHs were also to determine the ability to clear bacteria in a mouse pneumonia model.. AB - Synthetic disaccharide hydrocarbons (DSHs) that reactive bacterials swarming motility and inhibit bacterial adhesion and biofilm formation. A library of DSHs were tested in several experiment for the impact on various Pseudomonas aeruginosa populations and compared against existing compounds to determine efficacy and utility. Certain DSHs were also to determine the ability to clear bacteria in a mouse ...
TY - JOUR. T1 - T cell-mediated increased osteoclast formation from peripheral blood as a mechanism for crohns disease-associated bone loss. AU - Oostlander, A.E.. AU - Everts, V.. AU - Schoenmaker, T.. AU - Bravenboer, N.. AU - van Vliet, S.J.. AU - van Bodegraven, A.A.. AU - Lips, P.T.A.M.. AU - Vries, T.J.. PY - 2012. Y1 - 2012. U2 - 10.1002/jcb.23352. DO - 10.1002/jcb.23352. M3 - Article. C2 - 21898548. VL - 113. SP - 260. EP - 268. JO - Journal of Cellular Biochemistry. JF - Journal of Cellular Biochemistry. SN - 0730-2312. IS - 1. ER - ...
Bacteria love to colonize surfaces inside your body, but they have a hard time getting past your rugged, salty skin. Surgeries to implant medical devices often give such bacteria the opportunity needed to gain entry into ...
Diarrhoea in piglets due to Escherichia coli is an important problem in the pig farming industry. Adhesion of these bacteria to the epithelial cells of small intestine is an essential prerequisite for the incidence of diarrhoea. Among the putative candidate genes associated with adhesion pattern, TFRC (transferrin receptor) gene was localized on targeted region of SSC13 and considered as a positional candidate gene. The present investigation was conducted to evaluate the Indian desi pigs in terms of E. coli adhesion pattern (with Indian isolate) and study TFRC expression profile in different adhesion phenotypes. A total of 4 types of adhesion pattern were observed with different frequencies. RT-PCR analysis revealed that TFRC mRNA expression level was different across the differentially adhesive phenotypes. The increased level of TFRC expression indicates its role in immunity against E. coli mediated diarrhoea.
OBJECTIVE: The present study aimed to investigate the effects of the bacterial biofilm formation on the tonsil surface exposed N-acetyl-cysteine (NAC) and
Synthesis of diverse indole libraries on polystyrene resin - Scope and limitations of an organometallic reaction on solid supports
Bacterial adhesion to other bacteria, to eukaryotic cells, and to extracellular matrix proteins is frequently mediated by cell surface-associated polymers (fimbriae) consisting of one or more subunit proteins. We have found that polymerization of curlin to fimbriae-like structures (curli) on the surface of Escherichia coli markedly differs from the prevailing model for fimbrial assembly in that it occurs extracellularly through a self-assembly process depending on a specific nucleator protein. The cell surface-bound nucleator primes the polymerization of curlin secreted by the nucleator-presenting cell or by adjacent cells. The addition of monomers to the growing filament seems to be driven by mass action and guided only by the diffusion gradient between the source of secreted monomer and the surface of monomer condensation.. ...
Fluorescent antinuclear antibody (ANA) testing was performed on 141 sera from 114 patients with well defined rheumatic diseases including fibrositis syndrome and 24 sera from 24 healthy subjects using HEp-2 cells and rat liver as substrates. ANA titers were almost always higher on HEp-2, in most cas …
immune Uncategorized Bosutinib, LDHAL6A antibody Bacterial biofilm has been shown to play a role in delaying wound healing of chronic wounds, a major medical problem that results in significant healthcare burden. gradually cleared from your wounds while the presence of (part of the normal mouse pores and skin flora) improved. Scabs from all unhealed wounds contained 107 study of bacterial biofilm reactions to sponsor defenses and the effects of biofilms on sponsor wound healing pathways. It may also be used to test anti-biofilm strategies the treatment of chronic wounds. spp., and [5C7] have been isolated from chronic wounds, even though the wound may not display any medical indications of localized illness. Multiple bacterial varieties, usually two to five varieties, reside concurrently on a single ulcer [7C9]. The chronicity of unhealed wounds is definitely associated with higher proportion of colonization by anaerobic bacteria and greater variety of aerobic varieties [5]. More recent studies ...
Ideal Bowel Support® LP299v® contains a clinically-documented, human-origin probiotic strain, L. plantarum 299v, that resists stomach acid and bile salts and demonstrated specific adherence properties for colonization of human intestinal mucosa.* L. plantarum 299v has been used in human clinical studies for intestinal
Video photo micrography has been of enormous importance in deciphering the events surrounding the attachment of bacteria to surfaces. The best evidence suggests that attachment occurs as a two-step process. Reversible attachment, which is tenuous and often transient and irreversible attachment that is much more stable. In some cases, following their initial attachment to a surface the bacteria can be seen to be rapidly spinning, vibrating or actually moving across the substrate surface. Spinning is an indication that the cells are attached to the substrate by their flagella, but since the flagellum is now "fixed", the cell body rotates as the flagellar motor continues to rotate. Sometimes, cells in contact with the surface seem to be vibrating, that is they areexhibiting Brownian motion, caused by the constant and random impact of water molecules striking the loosely attached bacterial cell.Videos of Pseudomonas aeruginosa reveal that after initial attachment, cells move over the surface with a ...
Pseudomonas aeruginosa is an opportunistic human pathogen that forms biofilm infections in a wide variety of contexts. Biofilms initiate when bacteria attach to a surface, which triggers changes in gene expression leading to the biofilm phenotype. We have previously shown, for the P. aeruginosa lab strain PAO1, that the self-produced polymer Psl is the most dominant adhesive for attachment to the surface but that another self-produced polymer, Pel, controls the geometry of attachment of these rod-shaped bacteriastrains that make Psl but not Pel are permanently attached to the surface but adhere at only one end (tilting up off the surface), whereas wild-type bacteria that make both Psl and Pel are permanently attached and lie down flat with very little or no tilting (Cooley et al 2013 Soft Matter 9 38716). Here we show that the change in attachment geometry reflects a change in the distribution of Psl on the bacterial cell surface. Bacteria that make Psl and Pel have Psl evenly coating the ...
Compared to asymptomatic UTI, significantly more number of bacteria adhered to the epithelial cells of women with symptomatic UTI (P< 0.001). All cases of UTI had significantly high concentration of urinary IgG antibody to mixed coliform antigens. Asymptomatic UTI cases had higher concentrations of urinary IgG, IgM and IgA antibodies to clinical isolate. Concentration of sIgA level was more in symptomatic UTI. Significant correlation was observed between urinary IgG and adherence of clinical isolate in case of asymptomatic UTI ...
The Asally lab studies the spatio-temporal dynamics of bacterial biofilm formation in vitro and in vivo by taking synthetic and systems biology approaches.
Adhesins are surface structures of bacteria that facilitate their attachment to host cells or non-living materials. New research has advanced our understanding of adhesins, as outlined in a new report ...
Family built after PMID=25023666; The GT101 module of Streptococcus parasanguinis dGT1 catalyzes the transfer of glucose to the branch point of the hexasaccharide O-linked to the serine-rich repeat of the bacterial adhesin Fap1 ...
In article ,2df8d690 at rohmhaas.com,, Kenneth_M_Wiencek at ROHMHAAS.COM (Kenneth M Wiencek) wrote: , Heres a question to ponder... , , What is a biofilm anyway? Is one attached cell a biofilm? 100? , 1000000? A monolayer? Is a 3-D structure a requirement for a , biofilm? .....[edit]....if the characteristics of cells change , immediately upon attachment (a physiological response to being , attached such as increased resistance to antimicrobials), then perhaps , a biofilm could be one attached cell (a biofilm onto itself) It seems to me that combining one attached cell with the exponential growth equation necessarily implies the presence of a large multicell film after not very many generations (or one dead attached cell if the growth rate constant is negative). Single cells are irrelevant except as progenitors for populations. -- R.A.Preston rapr at med.pitt.edu ...
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This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
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