Comparative 16S rRNA (rDNA) sequence analyses performed on the thermophilic Bacillus species Bacillus acidocaldarius, Bacillus acidoterrestris, and Bacillus cycloheptanicus revealed that these organisms are sufficiently different from the traditional Bacillus species to warrant reclassification in a new genus, Alicyclobacillus gen. nov. An analysis of 16S rRNA sequences established that these three thermoacidophiles cluster in a group that differs markedly from both the obligately thermophilic organism Bacillus stearother-mophilus and the facultatively thermophilic organism Bacillus coagulans, as well as many other common mesophilic and thermophilic Bacillus species. The thermoacidophilic Bacillus species B. acidocaldarius, B. acidoterrestris, and B. cycloheptanicus also are unique in that they possess ω-alicylic fatty acid as the major natural membranous lipid component, which is a rare phenotype that has not been found in any other Bacillus species characterized to date. This phenotype, along with
Definition of bacillus globigii in the Definitions.net dictionary. Meaning of bacillus globigii. What does bacillus globigii mean? Information and translations of bacillus globigii in the most comprehensive dictionary definitions resource on the web.
Category: Animal, Bacillus, Probiotics. Paenibacillus polymyxa is a Gram-positive bacterium capable of fixing nitrogen. The species may also beknown as Bacillus polymyxa. It is found in soil, plant roots, and marine sediments.Bacillus polymyxa is a Gram-positive bacterium capable of fixing nitrogen.. ...
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|15614886|ref|NP_243189.1| from Bacillus halodurans C-125. Plus protein sequence and external database links.
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Bacillus sporothermodurans, which was first detected in UHT milk in Germany in 1990, can affect the stability and shelf life of contaminated commercial UHT milk and cause economic losses. Due to the unusual thermal resistance of B. sporothermodurans spores, B. sporothermodurans can survive UHT treatment and proceed to grow in stored products causing instability because of their proteolytic activity (Huemer et al., 1998). This study was conducted to determine the level of B. sporothermodurans contamination in South African dairies and to understand the mechanism of B. sporothermodurans spore destruction in order to investigate ways of inactivating these spores without severe heating. The objectives were to determine the presence of Bacillus sporothermodurans in retail UHT milk along with milk from different points of a processing line and isolates from UHT milk in South Africa, UP20A and a reference strain of B. sporothermodurans, DSM 10599 from Deutsche Sammlung von Mikroorganismen und ...
Summary: Flagella from Bacillus firmus RAB, an alkalophilic bacterium, were purified to homogeneity. The flagella were shown to consist of a single protein subunit (flagellin) with an apparent molecular weight of 40000. The amino acid composition of B. firmus RAB flagellin was similar to that of other bacilli except that the former had far fewer basic amino acids. The paucity of basic amino acics may render the flagella more stable at external pH values as high as 11.0.
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Electron micrographs of negatively stained preparations of cell walls of Bacillus polymyxa have been investigated by optical diffraction and optical filtering techniques. Images of single layers of the cell wall, from which the noise has been filtered optically, show hollow, square-shaped morphological units arranged on a square lattice of side 100 Å. Single-layer images showing the same pattern have been filtered from moiré patterns arising from two overlapping single layers. The morphological units are composed of four smaller subunits. The optical diffraction patterns from regions of two overlapping layers show extra reflexions which are attributed to multiple electron scattering. ...
Bacillus licheniformis ATCC ® 14580D-5™ Designation: Genomic DNA from Bacillus licheniformis Strain 46 TypeStrain=True Application:
The acid-soluble products of exhaustive digestion of native DNA with Bacillus laterosporus DNase consist of 6.5% of mononucleotides and 93.5% of oligonucleotides with an average chain length of 3.2. The results of viscometric studies and inactivation of transforming DNA indicate the exi...read more ...
Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
1. The properties of membrane vesicles from the extreme thermophile Bacillus caldolyticus were investigated. 2. Vesicles prepared by exposure of spheroplasts to ultrasound contained cytochromes a, b and c, and at 50°C they rapidly oxidized NADH and ascorbate in the presence of tetramethyl-p-phenylenediamine. Succinate and l-malate were oxidized more slowly, and dl-lactate, l-alanine and glycerol 1-phosphate were not oxidized. 3. In the absence of proton-conducting uncouplers the oxidation of NADH was accompanied by a net translocation of H+ into the vesicles. Hydrolysis of ATP by a dicyclohexylcarbodi-imide-sensitive adenosine triphosphatase was accompanied by a similarly directed net translocation of H+. 4. Uncouplers (carbonyl cyanide p-trifluoromethoxyphenylhydrazone or valinomycin plus NH4+) prevented net H+ translocation but stimulated ATP hydrolysis, NADH oxidation and ascorbate oxidation. The last result suggested an energy-conserving site in the respiratory chain between cytochrome c ...
Using an insect model to assess correlation between temperature and virulence in Bacillus weihenstephanensis and Bacillus cereus ...
Abstract The challenge of maintaining a cytoplasmic pH that is much lower than the external pH is central to the adaptation of extremely alkaliphilic Bacillus species to growth at ..
Domain architecture and assignment details (superfamily, family, region, evalue) for gi|163941511|ref|YP_001646395.1| from Bacillus weihenstephanensis KBAB4. Plus protein sequence and external database links.
Bacillus amyloliquefaciens strain FZB42 is a free-living bacterium that competitively colonizes plant roots and stimulates plant growth by many different modes of action. The molecular basis of singular beneficial effects that this Plant Growth-Promoting Rhizobacteria (PGPR) exert on their hosts have been studied. To decipher the molecular cross-talk of B. amyloliquefaciens and its host plants as a whole system, an extensive proteomic approach was performed. Reference maps of the extracellular and cytosolic protein fractions were established. The highest number of secreted proteins was observed during stationary growth phase. Identified extracellular proteins belong to different functional classes, with the most prominent classes involved in carbohydrate degradation and transportation of molecules across the cell wall. Cytosolic extracts obtained from cultures grown in 1C and minimal media subjected to the 2 Dimensional Electrophoresis (2 DE), revealed 461 and 245 different protein entries, ...
Fig. S1. Predicted secondary structures of the tmRNA and associated group I intron in the chromosome of B. pseudofirmus OF4. The structures were predicted by alignment with the homologous sequences in C. botulinum (Williams, 2002) followed by manual rearrangements. Nucleotides that are conserved in both sequence and structural position in B. pseudofirmus and C. botulinum are coloured in red. The various subdomains of the tmRNA (P1-P12) and group I intron (P1-P9) are indicated. In addition, for the tmRNA, the regions forming pseudoknots are labelled (Ψ1 and Ψ4) the nucleotides encoding the proteolysis tag are underlined; for the intron, flanking exon sequences are in lower case letters.. Fig. S2. Schematic representation of the 4.5 kb group II twintron in the pBpOF4-01 plasmid of B. pseudofirmus OF4. The twintron consists of two nested group II intron retroelements, where the innermost intron (in grey) encoding the reverse transcriptase (RT) BpOF4_20224 is inserted at the catalytic site of the ...
Bacillus amyloliquefaciens FZB42 is a Gram-positive, pathogen-suppressing and plant-growth promoting rhizobacterium. Apart from this ability, it produces a vast array of secondary metabolites, which includes both ribosomal and non-ribosomal peptides. In this work, the transcriptional activation and regulation of bacilysin biosynthesis were studied at the promoters of bac and ywfH genes. The promoter of bacilysin was identified using 5-deletion analysis. Sigma factor A (σA) was found to start transcription via conserved promoter elements (-10 and -35) of bac and ywfH genes. lacZ reporter fusion studies were performed in wild type and regulatory mutants. The results show the involvement of transcriptional regulators to activate the expression of bacilysin genes. Several global regulators such as DegU, ComA, Hpr and AbrB were identified and found to influence gene expression. In particular, I confirmed DegU binding in bac and ywfH promoters using radioactive DNase I footprinting. Furthermore, ...
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Here we announce a 3.0-Mb assembly of the Bacillus coagulans Hammer strain, which is the type strain of the species within the genus Bacillus. Genomic analyses based on the sequence may provide insights into the phylogeny of the species and help to elucidate characteristics of the poorly studied strains of Bacillus coagulans.
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Consuming large doses of Bacillus coagulans deemed safe for humans. CLEVELAND (April 7, 2009) - The results of a safety study to be published in the May issue of Food and Chemical Toxicology demonstrate that the probiotic strain, GanedenBC30 (Bacillus coagulans GBI-30, 6086), is safe for human consumption, even in massive amounts. Many experts recognize the health benefits of probiotics, but some have stressed that probiotic strains must first demonstrate safety before recommendations can be made about their widespread use. The new study also sets the stage for the incorporation of GanedenBC30 into a wide variety of foods. Unlike many other probiotic strains, GanedenBC30 easily survives the challenges of food manufacturing, extreme temperatures, and the gastric environment.. Probiotics, also referred to as friendly bacteria, are becoming increasingly popular with consumers for several health benefits. However, safety studies do not exist for many strains of probiotics because there are no ...
Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) [ME-CELBA] - High purity recombinant Cellulase (endo-1,4-β-D-glucanase) (Bacillus amyloliquefaciens) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. EC 3.2.1.4 CAZy Family: GH5 Recombinant. From Bacillus amyloliquefaciens. In 3.2 M ammonium sulphate. Specific activity: ~ 80 U/mg (40oC, pH 6.0 on CM-cellulose 4M); ~ 160 U/mg (60oC, pH 6.0 on CM-cellulose 4M). Stability: | 2 years at 4oC.
Novel Gram-negative alkaliphilic strains were isolated from soil obtained from Atsuma, Hokkaido, Japan. The isolates were strictly aerobic rods that produced subterminally located ellipsoidal spores. Chemotaxonomic characteristics of the isolates included the presence of meso-diaminopimelic acid in the cell wall and a DNA G+C content of 40·2-40·9 mol%. The major isoprenoid quinone was menaquinone-7 and the cellular fatty acid profile consisted of a significant amount of 15-C branched-chain acids, iso-C15:0 and anteiso-C15:0. The growth rate was higher at pH 8-10 than at pH 7. Comparative sequence analysis of 16S rDNA of 14 alkaliphilic Bacillus strains indicates that the isolated strain has an equidistant relationship to three already defined rRNA groups of alkaliphilic Bacillus species. Based on the morphological and physiological characteristics, as well as phylogenetic position as determined by 16S rDNA analysis and DNA-DNA relatedness data, it is concluded that these isolates should be designated
TY - JOUR. T1 - ATP synthesis by the F0F1 ATP synthase from thermophilic Bacillus PS3 reconstituted into liposomes with bacteriorhodopsin. T2 - 2. Relationships between proton motive force and ATP synthesis. AU - Pitard, Bruno. AU - Richard, Peter. AU - Duñach, Mireia. AU - Rigaud, Jean Louis. PY - 1996/2. Y1 - 1996/2. N2 - The correlation between the rate of ATP synthesis and light-induced proton flux was investigated in proteoliposomes reconstituted with bacteriorhodopsin and ATP synthase from thermophilic Bacillus PS3. By variation of the actinic light intensity it was found that ATP synthase activity depended in a sigmoidal manner on the amplitude of the transmembrane light-induced pH gradient. Maximal rates of ATP synthesis (up to 200 nmol ATP · min-1 · mg protein-1 were obtained at saturating light intensities under a steady-state pH gradient of about pH 1.25. It was demonstrated that this was the maximal ΔpH attainable at 40°C in reconstituted proteoliposomes, due to the feedback ...
During the last decade, the use of plant-root colonizing bacteria with plant growth-promoting activity has been proven as an efficient and environmental-friendly alternative to chemical pesticides and fertilizers. Biofertilizer and biocontrol formulations prepared from endospore-forming Bacillus strains are increasingly applied due to their long shelf life, which is comparable with that of agrochemicals. Today, spore suspensions from natural representatives of mainly Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus pumilus are available. However, these biofertilizers, directly prepared from environmental strains, are sometimes hampered in their action and do not fulfill in each case the expectations of the appliers (Borriss R, Bacteria in agrobiology: plant growth responses, Springer, 2011, pp. 41-76). This review will focus on several ways to improve the action of B. amyloliquefaciens subsp. plantarum FZB42T, the type strain for the group of plant-associated B. amyloliquefaciens strains. We
Previous DNA relatedness studies showed that strains idnentified as Bacillus mycoides segregated into two genetically distinct yet phenotypically similar groups, one being B. mycoides sensu stricto and the other, an unclassified taxon. In the present study, the taxonomic position of this second group was assessed by measuring DNA relatedness and determining phenotypic characteristics of an increased number of B. mycoides strains. Also determined was the second group's 16S RNA gene sequence. The 36 B. mycoides strains studied segregated into two genetically distinct groups showing DNA relatedness of about 30%; 18 strains represented the species proper and 18 the second group with intragroup DNA relatedness for both groups ranging from 70 to 100%. DNA relatedness to the type strains of presently recognized species with G+C contents of approximately 35 mol% (Bacillus alcalophilus, Bacillus cereus, Bacillus circulans, Bacillus lentus, Bacillus megaterium and Bacillus sphaericus) ranged from 22 to 37%.
Postharvest diseases of bananas remain a significant challenge of banana exporting countries and can result in considerable losses. Adequate storage and transportation facilities are among the strategies employed to reduce losses, but chemical control has been found to be more effective and economical. Synthetic fungicides have traditionally been used to mitigate postharvest diseases caused by harmful microorganisms. However, with current global health concern, there is a public desire to utilize eco-friendly alternatives to address decay loss. A study on the bioefficacy of a commercial formulation containing Bacillus amyloliquefaciens strain D203 (Hatake™) for control of crown rot disease in Cavendish bananas, was conducted using both in vitro and in vivo screening techniques. Its potential in controlling crown rot and the efficacy of different rates were determined. Crown rot causing microorganisms were isolated and tested for antagonism by B. amyloliquefaciens strain D203. Efficacy was ...
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Products, termed Antheridiogen Factor (AF), and vesicle like sac formation factor (VLS-F) were present in a culture of Bacillus amyloliquefaciens stra..
TY - JOUR. T1 - Production of native-starch-degrading enzymes by a Bacillus firmus/lentus strain. AU - Wijbenga, Dirk-Jan. AU - Beldman, Gerrit. AU - Veen, Anko. AU - Binnema, Doede. PY - 1991/5/1. Y1 - 1991/5/1. N2 - A bacterium belonging to the Bacillus firmus/lentus-complex and capable of growth on native potato starch was isolated from sludge of a pilot plant unit for potato-starch production. Utilization of a crude enzyme preparation obtained from the culture fluid after growth of the microorganism on native starch, resulted in complete degradation of native starch granules from potato, maize and wheat at a temperature of 37°C. Glucose was found as a major product. Production of maltose, maltotriose and maltotetraose was also observed. Native-starch-degrading activity (NSDA) could be selectively adsorbed on potato-starch granules, whereas soluble-starch-degrading activity (SSDA) remained mainly in solution. The use of such a starch-adsorbed enzyme preparation on native starch resulted in a ...
Bacterial protease produced by submerged fermentation of a selected strain of Bacillus amyloliquefaciens. Secretion of protease by Bacillus amyloliquefaciens can be inhib
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Alkalophilic Bacillus include industrially important species since they can produce many extracellular enzymes which are active and stable at high pH values.These alkaline enzymes (proteases, amylases, xylanases, cellulases, lipases and pectinases) find use in various field of industry such as leather, detergent, paper industries and waste water treatment.Isolation of diverse bacteria plays an important role in finding novel enzymes with improved characteristics. The aim of this study was therefore to screen for alkaline extracellular enzymes of alkalophilic Bacillus isolated from soil, horse feces and leather processing and to characterize these strains by phenotypic tests and by 16S-ITS rDNA based RFLP.At the end of the study, rod-shaped, endospore forming and Gram positive 116 strains were identified as Bacillus. Ten of the 116 strains were found to be obligate alkalophilic. 91 protease, 77 amylase, 18 xylanase, 3 cellulase, 74 pectinolytic enzyme (71 polygalacturonic acid degrading and 72 ...
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Bacillus amyloliquefaciens strain H57 was initially isolated for its ability to inhibit the growth of fungi that causes livestock feed to spoil. When applied to feed, H57 markedly reduces fungal growth for up to three months.
Name: Pseudomonas weihenstephanensis von Neubeck et al. 2016. Category: Species. Proposed as: sp. nov.. Etymology: wei.hen.ste.pha.nensis N.L. masc./fem. adj. weihenstephanensis, pertaining to Freising/ Weihenstephan in southern Germany, where the type strain was isolated Gender: feminine Type strain: DSM 29166; LMG 28437; WS4993 See detailed strain information at ...
Bacillus Coagulans Probiotics Powder is a Gram-positive rod, catalase positive, spore-forming, motile, a facultative anaerobe. B. coagulans may appear Gram-negative when
Bacillus coagulans (B. coagulans) is a remarkably interesting type of good bacteria that is also a probiotic that produces lactic acid. This probiotic produces spores during a reproductive cycle, so it is able to be dormant and survive under circumstances that kill other probiotics.
... CLEVELAND Dec. 4 /- An independent panel of scientis...(Logo: A HREF http://www.newscom.com/cgi-bin/prnh/20041213/CLM031LOG... With so many health benefits and potential applications available for...GanedenBC30 (Bacillus coagulans GBI-30 6086) is a high-survivability...,Ganeden,Biotech,Obtains,Self-Affirmed,GRAS,Status,for,Probiotic,Ingredient,,GanedenBC30,(Bacillus,coagulans,GBI-30,,6086),biological,advanced biology technology,biology laboratory technology,biology device technology,latest biology technology
Despite its widespread use for roads, asphalt presents significant problems related to the environment (due to the use of bitumen compounds), sustainability (due to the use of oil) and its variable price, which makes it necessary to consider at least new materials for road construction. It is possible that the future of more sustainable and economical road construction lies in a bacterium called Bacillus Pasteurii, which combined with sand produces solid sandstone very quickly, forming a ready-to-use pavement ...
In addition to its use in food and cosmetics, lactic acid is increasingly used as a starting material for production of bio-based, renewable plastics [1-3]. Optically pure lactic acid required by the bioplastics industry is currently produced only by bacterial fermentation of sugars [3,4]. The main sugars currently used in such fermentations are glucose derived from corn starch or sucrose from sugar cane, sugar beets, etc. With increasing demand for renewable bio-based plastics, there is a shift away from food-based carbohydrates to non-food carbohydrates such as lignocellulosic biomass for lactic acid production [5,6]. Commercial fungal cellulases play a central role in the conversion of cellulose to glucose before fermentation to lactic acid and these enzymes function optimally at 50°C and pH 5.0 [7-10]. By matching the fungal enzyme activity optimum with that of the growth and fermentation optimum of the microbial biocatalyst, such as Bacillus coagulans, the amount of fungal cellulases ...
Looking for online definition of Bacillus polymyxa in the Medical Dictionary? Bacillus polymyxa explanation free. What is Bacillus polymyxa? Meaning of Bacillus polymyxa medical term. What does Bacillus polymyxa mean?
Introduction: Increased usage and improper management of electronic wastes result in immense environmental pollution. Although conventional techniques are well known for heavy metals removal from the environment, their high cost and severe environmental consequences indicate the urgent requirement of cost-effective methods of heavy metals uptake. Bioaccumulation can be considered as an alternative to the traditional methods in terms of their cost-effectiveness and maximum recovery of the metal ions. Materials and Methods: This study deals with the isolation of heavy metals tolerant Gram-positive bacterial strain, Bacillus licheniformis JAJ3, and its application in bioaccumulation of copper, lead, and nickel and bioleaching of heavy metals from electronic waste. 16S rRNA sequencing was performed to identify the bacterial strain. The accumulation study was carried out in a liquid medium and analyzed using atomic absorption spectroscopy. Bioleaching activity was checked using the one-step procedure. For
611536DNAArtificial SequenceDescription of Artificial Sequence Synthetic polynucleotide 1atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc 60ttgctgcctc attctgcagc ttcagcagca aatcttaatg ggacgctgat gcagtatttt 120gaatggtaca tgcccaatga cggccaacat tggaagcgtt tgcaaaacga ctcggcatat 180ttggctgaac acggtattac tgccgtctgg attcccccgg catataaggg aacgagccaa 240gcggatgtgg gctacggtgc ttacgacctt tatgatttag gggagtttca tcaaaaaggg 300acggttcgga caaagtacgg cacaaaagga gagctgcaat ctgcgatcaa aagtcttcat 360tcccgcgaca ttaacgttta cggggatgtg gtcatcaacc acaaaggcgg cgctgatgcg 420accgaagatg taaccgcggt tgaagtcgat cccgctgacc gcaaccgcgt aatttcagga 480gaacacctaa ttaaagcctg gacacatttt cattttccgg ggcgcggcag cacatacagc 540gattttaaat ggcattggta ccattttgac ggaaccgatt gggacgagtc ccgaaagctg 600aaccgcatct ataagtttca aggaaaggct tgggattggg aagtttccaa tgaaaacggc 660aactatgatt atttgatgta tgccgacatc gattatgacc atcctgatgt cgcagcagaa 720attaagagat ggggcacttg gtatgccaat gaactgcaat tggacggttt ccgtcttgat 780gctgtcaaac acattaaatt ...
In this work, 246 Bacillus sphaericus strains were evaluated against Aedes aegypti and Culex quinquefasciatus larvae to select the most effective ones to be used as the basis of a national product. All strains were isolated from different regions of Brazil and they are stored in a Bacillus spp. collection at Embrapa Genetic Resources and Biotechnology. The selected strains were characterized by biochemical and molecular methods. Based on selective bioassays, 87 strains were identified as toxic to one or both target species. All of these strains contain genes that encode the 42, 51 kDa proteins that constitute the binary toxin and the 100 kDa Mtx1 toxin. All toxic strains presented a very high LC50 against A. aegypti, so, a product based on any of these B. sphaericus strains would not be recommended for use in programmes to control A. aegypti. S201 had highest activity against C. quinquefasciatus, presenting the lowest LC50 and LC90 in bioassays.. ...
TY - JOUR. T1 - Purification and characterization of a novel β-agarase from an alkalophilic bacterium, Alteromonas sp. E-1. AU - Kirimura, Kohtaro. AU - Masuda, Noriyoshi. AU - Iwasaki, Yousuke. AU - Nakagawa, Hiroyuki. AU - Kobayashi, Reijiro. AU - Usami, Shoji. PY - 1999/1/1. Y1 - 1999/1/1. N2 - A novel β-agarase (EC 3.2.1.81) was purified from an agar-degrading alkalophilic bacterium, Alteromonas sp. E-1 isolated from the soil. This enzyme was obtained from a cell-free extract after sonication and purified 40.9-fold through treatment with streptomycin, ammonium sulfate fractionation and successive chromatography on anion-exchange and gel filtration columns. The molecular weight was estimated to be 82 kDa by SDS-polyacrylamide gel electrophoresis and 180 kDa by Superdex 200 gel filtration. The enzyme was inhibited by Mn2+, Cu2+, Fe2+, Zn2+ and Hg2+, and activated by K+, Na+ and EDTA, and its optimum pH and temperature for agarose degradation were 7.5 and 40°C, respectively. This ̄-agarase ...
TY - JOUR. T1 - Characterization of a Mannose-6-Phosphate Isomerase from Bacillus amyloliquefaciens and Its Application in Fructose-6-Phosphate Production. AU - Sigdel, Sujan. AU - Singh, Ranjitha. AU - Kim, Tae Su. AU - Li, Jinglin. AU - Kim, Sang Yong. AU - Kim, In Won. AU - Jung, Woo Suk. AU - Pan, Cheol Ho. AU - Kang, Yun Chan. AU - Lee, Jung Kul. N1 - Publisher Copyright: © 2015 Baikara et al. Copyright: Copyright 2015 Elsevier B.V., All rights reserved.. PY - 2015/7/14. Y1 - 2015/7/14. N2 - The BaM6PI gene encoding a mannose-6-phosphate isomerase (M6PI, EC 5.3.1.8) was cloned from Bacillus amyloliquefaciens DSM7 and overexpressed in Escherichia coli. The enzyme activity of BaM6PI was optimal at pH and temperature of 7.5 and 70°C, respectively, with a kcat/Km of 13,900 s-1 mM-1 for mannose-6-phosphate (M6P). The purified BaM6PI demonstrated the highest catalytic efficiency of all characterized M6PIs. Although M6PIs have been characterized from several other sources, BaM6PI is ...
Fingerprint Dive into the research topics of Thermotolerant Bacillus licheniformis TY7 produces optically active l-lactic acid from kitchen refuse under open condition. Together they form a unique fingerprint. ...
Asghari SM,Khajeh K,Ranjbar B,Sajedi RH,et al. (2004). Comparative studies on trifluoroethanol (TFE) state of a thermophilic alpha-amylase and its mesophilic counterpart: limited proteolysis, conformational analysis, aggregation and reactivation of the enzymes. Int. J. Biol. Macromol. 34: 173-179. http://dx.doi.org/10.1016/j.ijbiomac.2004.03.006 PMid:15225989 Azad MA, Bae JH, Kim JS, Lim JK, et al. (2009). Isolation and characterization of a novel thermostable alpha-amylase from Korean pine seeds. N. Biotechnol. 26: 143-149. http://dx.doi.org/10.1016/j.nbt.2009.09.006 PMid:19772955 Declerck N, Machius M, Wiegand G, Huber R, et al. (2000). Probing structural determinants specifying high thermostability in Bacillus licheniformis alpha-amylase. J. Mol. Biol. 301: 1041-1057. http://dx.doi.org/10.1006/jmbi.2000.4025 PMid:10966804 Dong Y,Liu Y,Chen Y,Niu D,et al. (2008). Purification and characterization of thermostable amylases from two bacterial species. Wei Sheng Wu Xue Bao 48: 169-175. ...
Several alkalophilic Bacillus spp. strains were selected for their capacity to produce alkaline cellulases. Culture supernatants of these strains showed optimal cellulase activities between pH 8 and 9 and they were stable from pH 6 to pH 12. A cellul
There are a number of methods in which heterologous peptides and proteins can be displayed on the cell surface of bacteria. The first use of the FliC system for display was carried out by Kuwajima et al. [1] where an eleven amino acid epitope from the egg-white lysozyme was displayed on the surface of E. coli. Ezaki et al [2] and Tanskanen et al [3] both demonstrated that large polypeptides could also be displayed successfully using E. coli flagellin. These proteins included an alkaline phosphatase (471 aa) and the collagen binding region of YadA of Yersinia enterolcolitica (302 aa) respectively. To our knowledge, the display of heterologous proteins using the FliC protein from flagella has not previously been demonstrated in Gram-positive bacteria. Bacillus halodurans Alk36 is an alkalophilic, Gram-positive bacterial strain which has the inherent capability of over-expressing the FliC protein. This was harnessed as an opportunity for the development of a novel surface display system using this ...
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Lactic acid producing bacteria (such as Bacillus coagulans) probiotics demonstrate immunomodulating and anti inflammatory effects and the ability to lessen the symptoms of arthritis in humans.. Bacillus Coagulans: A Viable Adjunct Therapy For Relieving Symptoms Of Rheumatoid Arthritis According To A Randomised, Controlled Trial. BMC Complementary and Alternative Medicine 2010, 10:1. David R Mandel, Katy Eichas, Judith Holmes.. The results of this pilot study suggest that adjunctive treatment with Bacillus coagulans probiotic appeared to be safe and effective for patients suffering from Rheumatoid Arthritis. The trial results showed that Bacillus coagulans improved pain symptoms and increased mobility in subjects with Rheumatoid Arthritis.. ...
article{139117, author = {Remaut, Han and SAFAROV, N and CIURLI, S and Van Beeumen, Jozef}, issn = {0021-9258}, journal = {JOURNAL OF BIOLOGICAL CHEMISTRY}, language = {eng}, number = {52}, pages = {49365--49370}, title = {Structural basis for Ni2+ transport and assembly of the urease active site by the metallochaperone UreE from Bacillus pasteurii.}, volume = {276}, year = {2001 ...
Kat has been writing about veganism, environment, and sustainability for five years. Their interests include over-analyzing the various socioeconomic forms of oppression, how that overlaps with veganism, and how the media in all of its forms reflects the current culture. Vegan and cruelty-free beauty brand Pacifica just dropped its latest collection at Target - and it includes drinkable probiotic powder you can add to water, juice, or smoothies. The new collection features four Beauty Powders, each of which contains vegan superfood ingredients said to benefit the body and mind. Seek Balance is a coconut vanilla-flavored powder packed with plant-based probiotics (bacillus coagulans, to be exact), antioxidant-rich berry powder, and coconut oil to benefit your gut health. Slay All Day contains pineapple powder, ashwaganda (a medicinal herb used in Ayurvedic medicine thats said to reduce stress), green tea, and hyaluronic acid to help you stay calm and keep your skin healthy. Glow Greens helps you ...
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Product Name:alpha-Galactosidase 4A from Bacillus halodurans CAS Number: Catalouge Number:BIZY1000 Purity: Commodity Code:99999999 MDL Number: Notes:alpha-Galactosidases are enzymes that participate in the hydrolysis of terminal, non-reducing alpha-D-galactose. Family: GH4. E.C.:3.2.1.22. Architecture: GH4. Main activity:alpha-Galactosidase. Synonyms:
Bacillus amyloliquefaciens ssp. plantarum strains as potential protective starter cultures for the production of Bikalga, an alkaline fermented food ...
The consequence of simultaneous and sequential inoculation of T. asperellum and B. amyloliquefaciens cultures with respect to growth rate, differential expression of vital genes and metabolites were examined. The competition was observed between T. asperellum and B. amyloliquefaciens under co-cultivation. The proliferation of Trichoderma was reduced in the simultaneous inoculation (TB1) method, possibly due to the fastest growth of Bacillus. Both T. asperellum and B. amyloliquefaciens were proliferated in sequential inoculation method (TB2). The sequential inoculation method (TB2) upregulated the expression of metabolites and vital genes (sporulation, secondary metabolites, mycoparasitism enzymes and antioxidants) in Trichoderma and downregulated in Bacillus and vice versa in co-inoculation method (TB1). The metabolic changes in the co-culture promoted the maize plant growth and defense potential under normal and biotic stress conditions. The metabolites produced by the co-culture of T. asperellum and B
Article Biological decolorization of industrial dyes by |italic|Candida tropicalis|/italic| and |italic|Bacillus firmus|/italic|. Disperse dyes are chiefly used by textile industries for the coloration of polyester and cellulose triacetate and their ...
ID Q5WE67_BACSK Unreviewed; 277 AA. AC Q5WE67; DT 23-NOV-2004, integrated into UniProtKB/TrEMBL. DT 23-NOV-2004, sequence version 1. DT 25-OCT-2017, entry version 69. DE SubName: Full=ATP-dependent DNA ligase {ECO:0000313,EMBL:BAD65343.1}; DE EC=6.5.1.1 {ECO:0000313,EMBL:BAD65343.1}; GN Name=ligB {ECO:0000313,EMBL:BAD65343.1}; GN OrderedLocusNames=ABC2809 {ECO:0000313,EMBL:BAD65343.1}; OS Bacillus clausii (strain KSM-K16). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus. OX NCBI_TaxID=66692 {ECO:0000313,EMBL:BAD65343.1, ECO:0000313,Proteomes:UP000001168}; RN [1] {ECO:0000313,Proteomes:UP000001168} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=KSM-K16 {ECO:0000313,Proteomes:UP000001168}; RA Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., RA Kawai S., Ito S., Horikoshi K.; RT The complete genome sequence of the alkaliphilic Bacillus clausii RT KSM-K16.; RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases. CC ...
Abstract: A factorial design experiment was conducted on field to investigate the effects of inoculation with AM fungi (Glomus mosseae), phosphate solubilizing bacteria strain (Bacillus polymyxa) and soil yeast strain (Saccharomyces cerevisiae) on growth, phosphorus content and yield of onion plants in calcareous soil under different levels of phosphorus fertilization (P1: 50, P2: 100 and P3: 200 kg ha-1). Results showed that inoculation with G. mosseae or B. polymyxa significantly (p = 0.05) increased plant height, shoot fresh and dry weights, root fresh and dry weights, average bulb diameter and total yield. The highest total yield and average bulb diameter were obtained from the inoculation treatment of AM fungus G. mosseae, recording 14.4 and 40.8% increases, respectively over the uninoculated control. Also, inoculation with AM fungus (G. mosseae) had a significant increase in all mineral content in onion plant compared with uninoculated control or other biofertilizers inoculation treatments ...
The objectives of this investigation were to produce a novel chitosanase for application in industries and waste treatment. The transformation of chitinous biowaste into valuable bioactive chitooligomers (COS) is one of the most exciting applications of chitosanase. An amphiprotic novel chitosanase from Bacillus mycoides TKU038 using squid pen powder (SPP)-containing medium was retrieved from a Taiwan soil sample, which was purified by column chromatography, and characterized by biochemical protocol. Extracellular chitosanase (CS038) was purified to 130-fold with a 35% yield, and its molecular mass was roughly 48 kDa. CS038 was stable over a wide range of pH values (4-10) at 50 °C and exhibited an optimal temperature of 50 °C. Interestingly, the optimum pH values were estimated as 6 and 10, whereas CS038 exhibited chitosan-degrading activity (100% and 94%, respectively). CS038 had Km and Vmax values of 0.098 mg/mL and 1.336 U/min, separately, using different concentrations of water-soluble chitosan. A
CLEVELAND, OH (January 8, 2008) - New Jersey based ingredient supplier, P.L. Thomas (PLT) announced today that it has partnered with Ganeden, Inc to represent the probiotic ingredient, GanedenBC30 (Bacillus coagulans GBI-30, 6086) to the food and beverage industry.. GanedenBC30 (Bacillus coagulans GBI-30, 6086) is a kosher, self-affirmed GRAS certified high-survivability strain of the probiotic, Bacillus coagulans. In addition to food products, GanedenBC30 is currently used in a broad range of applications including dietary supplements, animal health products, feminine hygiene and topical applications. GanedenBC30 is unique because it is shielded by a natural organic layer which protects the probiotic cells from the harsh manufacturing processes, do not require refrigeration and survive gastric acidity. Overall, GanedenBC30 has shown to be heartier than other probiotic strains and exhibits excellent stability, offering more flexibility in manufacturing and delivery systems.. GanedenBC30 can be ...
This is a bit of fun I did with my children to show them the dangers of eating too many sweets. Bacteria are commonly used as poweful sensors for toxins and environmental pollutants so for this experiment I fed some Gummy Bear sweets to the spreading soil bacterium Bacillus mycoides. As you can see from…
Deo, Namita and Natarajan, KA and Somasundaran, P (2001) Mechanisms of adhesion of Paenibacillus polymyxa onto hematite, corundum and quartz. In: International Journal of Mineral Processing, 62 (1-4). pp. 27-39. Natarajan, KA and Deo, Namita (2001) Role of bacterial interaction and bioreagents in iron ore flotation. In: International Journal of Mineral Processing, 62 (1-4). pp. 143-157. Deo, Namita and Natarajan, KA and Rao, Hanumantha K and Forssberg, KSE (1999) Biodegradation of some organic reagents from mineral process effluents. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International Biohydrometallurgy Symposium, JUN 20-23, 1999, Madrid, Spain. Deo, Namita and Vasan, SS and Modak, Jayant M and Natarajan, KA (1999) Selective biodissolution of calcium and iron from bauxite in the presence of Bacillus polymyxa. In: Biohydrometallurgy and the Environment Toward the Mining of the 21st Century - Proceedings of the International ...
2.4x) was achieved between PM and 1-MM duplexes at the dissociation temperature at which 50% of the probe-target duplexes remained intact. This provided excellent differentiation among representatives of different Bacillus species, both individually and in mixtures of two or three. The overall pattern of hybridization derived from this hierarchical probe set also provided a clear chip fingerprint for each of these closely related Bacillus species.>. Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach. Liu WT, Mirzabekov AD, Stahl DA. Environmental microbiology. 2001. Pubmed ...
2.4x) was achieved between PM and 1-MM duplexes at the dissociation temperature at which 50% of the probe-target duplexes remained intact. This provided excellent differentiation among representatives of different Bacillus species, both individually and in mixtures of two or three. The overall pattern of hybridization derived from this hierarchical probe set also provided a clear chip fingerprint for each of these closely related Bacillus species.>. Optimization of an oligonucleotide microchip for microbial identification studies: a non-equilibrium dissociation approach. Liu WT, Mirzabekov AD, Stahl DA. Environmental microbiology. 2001. Pubmed ...
Accurate enumeration of bacterial count in probiotic formulation is imperative to ensure that the product adheres to regulatory standards and citation in consumer product label. Standard methods like plate count, can enumerate only replicating bacterial population under selected culture conditions. Viable but non culturable bacteria (VBNC) retain characteristics of living cells and can regain cultivability by a process known as resuscitation. This is a protective mechanism adapted by bacteria to evade stressful environmental conditions. B. coagulans MTCC 5856(LactoSpore®) is a probiotic endospore which can survive for decades in hostile environments without dividing. In the present study, we explored the use of flow cytometry to enumerate the viable count of B. coagulans MTCC 5856 under acidic and alkaline conditions, high temperature and in commercial formulations like compressed tablets and capsules. Flow cytometry (FCM) was comparable to plate count method when the spores were counted at
A comprehensive metabolic network that considers 147 reaction fluxes and 105 metabolites is used in a mass-Aux-balance-based stoichiometric model for Bacillus licheniformis for serine alkaline protease (SAP) overproduction. The theoretical capacity analysis leading to optimized SAP overproduction was carried out by using a linear constrained optimization technique for several specific growth rates and the variation of the fluxes were calculated by fixing the sole carbon source citrates uptake rate at 10 mmol/gDW/h. The theoretical data-based capacity analysis was conducted by using the model in combination with the off-line extracellular analyses of the dry cell and the metabolites that were citrate, organic acids, amino acids, and SAP; and the variations in the intracellular fluxes were obtained for the three periods of the batch bioprocess. The flux distribution maps of the analyses showed that the tricarboxylic acid cycle was active and the cells utilized the gluconeogenesis pathway, the ...
Supported by crystallography studies, secreted ribonuclease of|i| Bacillus pumilus|/i| (binase) has long been considered to be monomeric in form. Recent evidence obtained using native polyacrylamide gel electrophoresis and size-exclusion chromatography suggests that binase is in fact dimeric. To eliminate ambiguity and contradictions in the data we have measured conformational changes, hypochromic effect, and hydrodynamic radius of binase. The immutability of binase secondary structure upon transition from low to high protein concentration was registered, suggesting the binase dimerization immediately after translocation through the cell membrane and leading to detection of binase dimers only in the culture fluid regardless of ribonuclease concentration. Our results made it necessary to take a fresh look at the binase stability and cytotoxicity towards virus-infected or tumor cells.
Bacillus sphaericus, an obligate alkalophile, overproduces extracellular alkaline proteases. Kinetics of growth and protease production by this organism were analyzed to identify parameters that control protease synthesis. Results showed protease production to be both growth and non-growth associated although a major portion of the enzyme was secreted in post exponential phases. The initial concentration of glucose and nitrogen sources significantly affected cell growth and enzyme secretion. Fed-batch cultures were performed to maximize protease activity in a bioreactor. By using suitable feeding strategies, the protease activity and its productivity in a fed-batch process was increased by 44% over that in batch process, primarily due to the longer maintenance of increased rates of growth and enzyme production by providing continuous and controlled supply of additional substrate and nutrients.. ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular amylase Amy RM44. Amy RM44 was purified to homogeneityon a single step by affinity chromatography using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS-PAGE and zymographic analysis. Nine foldpurification was achieved with the specific activity of 870 U/mg that provides the total yieldof the enzyme up to 31%. Studies on purified AmyRM44 characterization revealed that theoptimum temperature of enzyme was 100 ºC. Amy RM44 was proved to be highly thermostableas it retained 50% activity after 2 h at 100 ºC. Amy RM44 was stable over wide range of pHwith optimum activity at pH 5. Enzyme activity was not significantly inhibited by SDS andEDTA. Amy RM44 also exhibited its activity towards various carbohydrates such as dextrin,pullulan, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.
Biotechnologically produced 2,3-butanediol (2,3-BDO) is a potential starting material for industrial bulk chemicals such as butadiene or methyl ethyl ketone which are currently produced from fossil feedstocks. So far, the highest 2,3-BDO concentrations have been obtained with risk group 2 microorganisms. In this study, three risk group 1 microorganisms are presented that are so far unknown for an efficient production of 2,3-BDO. The strains Bacillus atrophaeus NRS-213, Bacillus mojavensis B-14698, and Bacillus vallismortis B-14891 were evaluated regarding their ability to produce high 2,3-BDO concentrations with a broad range of different carbon sources ...
Quorum sensing molecules (QSMs) are involved in the regulation of complicated processes helping bacterial populations respond to changes in their cell-density. Although the QS gene cluster (comQXPA) has been identified in the genome sequence of some bacilli, the QS system B. licheniformis has not been investigated in detail, and its QSM (ComX pheromone) has not been identified. Given the importance of this antagonistic bacterium as an industrial workhorse, this study was aimed to elucidate B. licheniformis NCIMB-8874 QS. The results obtained from bioinformatics studies on the whole genome sequence of this strain confirmed the presence of essential quorum sensing-related genes. Although polymorphism was verified in three proteins of this cluster, ComQ, precursor-ComX and ComP, the transcription factor ComA was confirmed as the most conserved protein. The cell-cell communication of B. licheniformis NCIMB-8874 was investigated through further elucidation of the ComX pheromone as 13-amino acid ...
A wall-plus-membrane preparation from a Bacillus licheniformis mutant incorporated radioactivity from a peptidoglycan precursor in which the free amino group of diaminopimelic acid was blocked by 14C-labelled acetyl group. This incorporation was penicillin-sensitive. The enzymically degraded product contained cross-linked dimers, showing that newly synthesized peptidoglycan chains had been cross-linked to the pre-existing cell wall.. ...
Bacillus licheniformis GL174 is a culturable endophytic strain isolated from Vitis vinifera cultivar Glera, the grapevine mainly cultivated for the Prosecco wine production. This strain was previously demonstrated to possess some specific plant growth promoting traits but its endophytic attitude and its role in biocontrol was only partially explored. In this study, the potential biocontrol action of the strain was investigated in vitro and in vivo and, by genome sequence analyses, putative functions involved in biocontrol and plant-bacteria interaction were assessed. Firstly, to confirm the endophytic behavior of the strain, its ability to colonize grapevine tissues was demonstrated and its biocontrol properties were analyzed. Antagonism test results showed that the strain could reduce and inhibit the mycelium growth of diverse plant pathogens in vitro and in vivo. The strain was demonstrated to produce different molecules of the lipopeptide class; moreover, its genome was sequenced, and analysis of the
J.M. Risse, K. Friehs, and E. Flaschel, Recycling of Biomass Resulting from Fermentation Processes with Bacillus licheniformis, Chem. Eng. Technol., vol. 24, 2001, pp. 141-145 ...
You Are Here: Branched chain amino acids maintain the molecular weight of poly(γ-glutamic acid) of Bacillus licheniformis ATCC 9945 during the fermentation. ...
Semantic Scholar extracted view of Large exopenicillinase, initial extracellular form detected in cultures of Bacillus licheniformis. by K. Izui et al.
1JWQ: The Structure of the catalytic domain of N-acetylmuramoyl-L-alanine amidase, a cell wall hydrolase from Bacillus polymyxa var.colistinus and its resemblance to the structure of carboxypeptidases
It is a single-celled organism that is part of the plant kingdom.This yeast is unique because it can act both as a bacterium and a fungus. In a healthy intestine, it exist as single cell form, called a bud. When the intestinal environment changes, the buds transform themselves into a filiform shape that allows penetration of the intestinal wall, with resulting colonization over the entire body. It is therefore very IMPORTANT to keep the candida transformation and the candida growth controlled by the presence of important bacteria such as Bacillus laterosporus BOD and the Lactobacillus Acidophilus, two very important beneficial intestinal bacteria. What does candida use for food?. As food passes through the stomach into the intestine, carbohydrates are broken down into simple sugars, which become the primary energy source for the body. The candida yeast robs the body of its primary energy source-sugar. candida also feeds on B vitamins to accelerate its growth. In this sense Candida actually be ...
Ubiquinone (UQ), also called coenzyme Q, and plastoquinone (PQ) are electron carriers in oxidative phosphorylation and photosynthesis, respectively. The quinoid nucleus of ubiquinone is derived from the shikimate pathway; 4-hydroxybenzoate is directly formed from chorismate in bacteria, while it can be formed from either chorismate or tyrosine in yeast. The following biosynthesis of terpenoid moiety involves reactions of prenylation, decarboxylation, and three hydroxylations alternating with three methylations. The order of these reactions are somewhat different between bacteria and yeast. Phylloquinone (vitamin K1), menaquinone (vitamin K2), and tocopherol (vitamin E) are fat-soluble vitamins. Phylloquinone is a compound present in all photosynthetic plants serving as a cofactor for photosystem I-mediated electron transport. Menaquinone is an obligatory component of the electron-transfer pathway in bacteria ...
BC30 is a unique and patented strain of probiotic with a protective, spore-like protein coating that allows it to withstand the harsh acidic environment of the stomach to reach the intestines. The benefits that have been clinically shown include:. • reducing minor abdominal discomfort and bloating*. • supporting key aspects of innate immune defense mechanisms*. In a study at an independent laboratory for survival in a simulated gastric environment, BC30 survived at least 100 times better compared to the leading probiotic product and leading yogurt products. The superior survivability enables BC30 to be temperature stable in formats which other probiotics may not be.. BC30 has a trusted safety profile ...
2UBP: A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels.