Background. Most extracellular virulence factors produced by Bacillus cereus are regulated by the pleiotropic transcriptional activator PlcR. Among strains belonging to the B. cereus group, the plcR gene is always located in the vicinity of genes encoding the YvfTU two-component system. The putative role of YvfTU in the expression of the PlcR regulon was therefore investigated.. Results. Expression of the plcR gene was monitored using a transcriptional fusion with a lacZ reporter gene in a yvfTU mutant and in its B. cereus ATCC 14579 parental strain. Two hours after the onset of the stationary phase, a stage at which the PlcR regulon is highly expressed, the plcR expression in the yvfTU mutant was only 50% of that of its parental strain. In addition to the reduced plcR expression in the yvfTU mutant, a few members of the PlcR regulon showed a differential expression, as revealed by transcriptomic and proteomic analyses. The virulence of the yvfTU mutant in a Galleria mellonella insect model was ...

Waggie, K S.; Hansen, C T.; Ganaway, J R.; and Spencer, T S., A study of mouse strain susceptibility to bacillus piliformis (tyzzers disease): the association of b-cell function and resistance. (1981). Subject Strain Bibliography 1981. 2192 ...

There is no correlation between the severity of anemia or the degree of thrombocytopenia and the severity of the renal disease. In addition, petechia, purpura, and/or active bleeding are uncommon features of HUS, despite the significant low platelet count.. This syndrome is more predominant in rural areas with a peak incidence in warmer seasons corresponding to the increased risk of E coli O157:H7 infection and is mostly presented as outbreaks (e.g., 1992 outbreak due to undercooked hamburger contaminated with E. coli). It is, generally, presented in children as gastroenteritis complaints (e.g., abdominal pain or tenderness, nausea or vomiting, fever), whereas affected adults may be asymptomatic.. E. coli O157:H7 is responsible for most of the typical HUS in children in the United States; several cases were reported following either gastrointestinal infections with different bacteria (e.g., shigella and salmonella) or nongastrointestinal infections. E. coli serotype O157:H7 is normally found as ...

The authors used to designate it just as b04i-3**T** or B04I-3**T** without KCTC number from the web site, probably because the information was registered to NCBI before the strain got an official KCTC number. A peroxide-degrading //​Bacillus//​ isolate (strain PLC9 =KACC 91464P) was named after it based on 100% 16S rRNA sequence similarity to KCTC 13219 (New bacteria //Bacillus nitroreducens//​ PLC9 with hydrogen peroxide-degrading activity with high survival rate in hydrogen peroxide. Appl Biochem Biotechnol 2013, 169:​701-711. PMID: [[http://​www.ncbi.nlm.nih.gov/​pubmed/​23271626,23271626 ...

MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 96.3) ...

MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 51.1) ...

Two strains of a Gram-positive catalase-negative, facultatively anaerobic coccus originating from human sources were characterized by phenotypic and molecular taxonomic methods. The strains were found to be identical to each other based on 16S rRNA gene sequencing and constitute a new subline within the genus Facklamia. The unknown bacterium was readily distinguished from Facklamis hominis and Facklamia ignava by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence it is proposed that the unknown bacterium be classified as Facklamia sourekii sp. nov., the type strain of which is CCUG 28783AT.

The most distinguishing feature of most members of the family Bacillaceae (phylum Firmicutes) is their ability to form endospores that provide high resistance to heat, radiation, chemicals, and drought, allowing these bacteria to survive adverse conditions for a prolonged period of time. Bacillaceae are widely distributed in natural environments, and their habitats are as varied as the niches humans have thought to sample. Over the years of microbiological research, members of this family have been found in soil, sediment, and air, as well as in unconventional environments such as clean rooms in the Kennedy Space Center, a vaccine-producing company, and even human blood ( 1 - 3 ). Moreover, members of the Bacillaceae have been detected in freshwater and marine ecosystems, in activated sludge, in human and animal systems, and in various foods (including fermented foods), but recently also in extreme environments such as hot solid and liquid systems (compost and hot springs, respectively), salt lakes, and

Biocompare product reviews can cover any kit, reagent, antibody, or piece of equipment you use in your lab and are a great forum for researchers seeking to determine if a particular product will work for them.. All you need is a unique image, protocol information, and some helpful notes or tips on how to best use the product or service.. Not only are reviews a valuable resource for researchers looking to save time and money but all reviews that are accepted for publication earns you an Amazon Gift Card!*. Click Here to Write Your Review for XpressBio. ...

Microbial utilization of uncommon C4 dicarboxylate L-tartrate is largely anaerobic, with aerobic L-tartrate utilization known for few bacterial species including Rhodopseudomonas sphaeroides and Pseudomonas putida. Aerobic L-tartrate-utilizing microbes could be industrially relevant owing to the efficient nature of the bioprocess and catalytic versatility of tartrate dehydrogenase (TDH) responsible for aerobic catabolism of L-tartrate. Present work involves isolation and characterization of Bacillus strains capable of aerobic L-tartrate utilization and its correlation with occurrence of TDH activity. Two out of 37 isolates, IC1-G and IC1-Y were identified as Bacillus megaterium spp. showing efficient aerobic growth, utilizing ~3.7 and 2.8 mM L-tartrate respectively at the end of 48 h. Several organic acids possibly including oxalic, succinic and citric acids were secreted as by-products of L-tartrate metabolism. Utilization of L-tartrate directly correlated with induction of TDH activity by ~3.2 ...

Spores of bacteria of Bacillus species are extraordinarily resistant to all manner of harsh treatments, and largely because of this resistance, spores of some Bacillus species are major agents of food spoilage and food-borne and other diseases (eg-Bacillus anthracis). The conversion of a dormant Bacillus spore into a vegetative bacterium by the process of spore germination is also a relatively simple differentiating system that is readily amenable to both biochemical and genetic analysis. While much has been learned in recent years on the mechanisms of spore resistance and germination, there is still much that is unknown. Dr. Setlows laboratory has ongoing multidisciplinary research projects attempting to determine: 1) the mechanisms involved in the extraordinary resistance of spores to heat and oxidizing agents; 2) the mechanism(s) of spore germination and its heterogeneity; and 3) the structure and organization of the inner membrane of spores. Methods used in these projects include: 1) ...

EFSA provides applicants with guidance on how to conduct safety assessment of feed additives containing or produced from Bacillus species. Bacillus species are bacteria commonly used in feed as probiotics or as sources of other feed additives. Some strains produce toxins that can cause food-borne diseases in humans, producing symptoms such as diarrhoea, nausea, or vomiting.

The Hippo signaling pathway was originally discovered in Drosophila melanogaster and has recently emerged as a potent regulator of cell proliferation and organ size (Badouel et al., 2009; Zhang et al., 2009b). Several components of the pathway act as tumor suppressors or as protooncogenes (Harvey and Tapon, 2007). Core components of the Hippo pathway include the upstream activator Merlin/Nf2 (Hamaratoglu et al., 2006), a gene that is mutated in tumors of nervous tissue (Trofatter et al., 1993; Ruttledge et al., 1994) and in renal cell carcinoma (Forbes et al., 2008; Morris and McClatchey, 2009; Dalgliesh et al., 2010), the Ser/Thr kinases MST1/2 (mammalian STE20 kinases 1 and 2) and Lats1/2 (large tumor suppressor 1 and 2), together with their coactivators WW45 and Mob. In the active state, Lats1/2 phosphorylates the transcriptional activators Yes-associated protein (YAP) and TAZ (transcriptional coactivator with PDZ-binding domain). This results in their cytoplasmic retention by binding to ...

Fong CS, Ozaki K, Tsou MB*. (2018). PPP1R35 ensures centriole homeostasis by promoting centriole-to-centrosome conversion. Mol Biol Cell. 29(23):2801-2808.. Yang TT, Chong WM, Wang WJ, Mazo G, Tanos B, Chen Z, Tran TMN, Chen YD, Weng RR, Huang CE, Jane WN, Tsou MB*, Liao JC*. (2018). Architecture of mammalian centriole distal appendages accommodates distinct blade and matrix functional elements. Nature Communication. 9(1):2023. (*Co-corresponding authors).. Shulman AS, Tsou MF*. (2017). Probing Cilia-Associated Signaling Proteomes in Animal Evolution. Dev Cell. 43(6):653-655.. Mazo G, Soplop N, Wang WJ, Uryu K, and Tsou MF*. (2016). Spatial control of primary ciliogenesis by subdistal appendages alters sensation-associated properties of cilia. Dev Cell. 39(4):424-437.. Kim M, ORourke BP, Soni RK, Jallepalli PV, Hendrickson RC, Tsou MF*. (2016). Promotion and suppression of centriole duplication are catalytically coupled through PLK4 to ensure centriole homeostasis. Cell Rep. ...

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Rhizosphere bacteria are one of the most potential biological control agents in the plant disease protection. Bacillus species as a group offer several advantages over other bacteria for protection against pathogens because of their ability to form endospores, and because of the broad-spectrum activity of their antibiotics. Five soil samples from tomato rhizosphere were collected from shambat area, Khartoum State, Sudan. Bacillus isolates were isolated from the rhizosphere of tomato to use as natural bio-control agents. They were screened for antagonism in vitro against Alternaria alternata causal agents of early blight disease of tomato. Serial dilution technique was adopted for the isolation of Bacillus species. Only 27 out of 45 Bacillus isolates showed antagonistic properties. Four out of the 27 isolates showed antagonism (Bacillus B25, B35, B41, B45) were identified to the species level by bacteriological assay (morphological and biochemical tests).

Discrimination by automatic ribotyping and random amplified polymorphic DNA PCR, RAPD, was compared for 40 different B. cereus dairy isolates, 4 different B. mycoides isolates and 6 culture collection strains. RAPD-PCR has previously shown to be useful for tracing contamination routes of B. cereus to milk. Automatic ribotyping using EcoRI and PvuII separated the B. cereus and B. mycoides isolates/strains into 36 different ribotypes. RAPD-typing with primers generated 40 different RAPD-profiles. However, 17 isolates clustered into eight groups, irrespective of the primer and restriction enzyme used, and in all but one case, the isolates with the same pattern were isolated from the same dairy. Automatic ribotyping proved to be a useful, standardized and quick method to discriminate between B. cereus strains, only slightly less discriminatory than RAPD-typing.. ...

Members of genus Gordonia are widely distributed in nature, and about 29 species have been identified. From 1996 to 2015, only 16 cases of infections caused by Gordonia sputi were reported worldwide, most of which were catheter related, such as contaminated central venous catheters and chest tubes, in a setting of immunocompromised status [7-9]. Gordonia spp. infection usually has a subacute or chronic course, sometimes resembling fungi infection. The patient in this case presented with vision blurred about 10 days after the iron foreign body penetrating, showing multiple clusters of white purulent lesions in the anterior chamber, vitreous cavity and on the retina, without obvious pain, which might clinically indicate a less virulent bacteria or fungi infection. According to the contemporary gram stain and its reaction to intravitreal antibiotic a gram-positive bacilli infection was presumed, and further molecular examinations confirmed the pathogen as Gordonia sputi. So, when facing a subacute ...

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Cellualse is one of the most important enzymes used in textile, detergent, paper, food and feed industries. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples. 24 soil samples were analyzed and 54 presumptive Bacillus isolates were isolated after heating the soil samples at 80°C for 10 min. Among them 45 isolates showed enzyme activity ranging from 0.003 to 0.17 U/ml in test tubes containing 5 ml medium composed of (g/L) glucose 0.5 gm, peptone 0.75 gm, FeSO4 0.01 gm, KH2PO4 0.5 gm, and MgSO4 0.5 gm at 120 rpm, 37° C and pH 7. Among them 1RW, 2WS, 3YR, 4WT, 6 RR, and 9SS showed 0.17, 0.15, 0.14, 0.15, 0.147 and 0.14U/ml enzyme activities, respectively. Production of cellulase by these isolates was further scaled up to shake culture containing 50 ml medium similar to that used in test tube culture. Among the isolates 1 RW showed the maximum activity. This 1 RW was identified by API kit and showed that 59 % belongs to ...

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Research underway with M.S. students; undergraduate students also involved to varying degrees:. Project #1 - Discovery of new viruses that infect dairy spoilage bacteria. M.S. student research project with expected completion date 5/2017. Current status = ~ 1⁄2 complete as of 5.20.2016.. Many Bacillus species are able to form spores, and therefore can survive some forms of pasteurization. In this study, soil samples around campus will be screened for novel bacterial-specific viruses, termed phages, which are specific to toxigenic strains of Bacillus. Once phages have been detected, they will be characterized using both SEM and TEM microscopy. They will also be DNA sequenced. These new phages could be used for detection and control of toxigenic strains of Bacillus in dairy foods, where they pose many costly problems during yogurt and cheese production. Using phages as a method for controlling spoilage causing and pathogenic bacteria is an idea that has recently been reintroduced in the dairy ...

J:74701 Hou X, Mrug M, Yoder BK, Lefkowitz EJ, Kremmidiotis G, DEustachio P, Beier DR, Guay-Woodford LM, Cystin, a novel cilia-associated protein, is disrupted in the cpk mouse model of polycystic kidney disease. J Clin Invest. 2002 Feb;109(4):533-40 ...

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Your basket is currently empty. i ,p>When browsing through different UniProt proteins, you can use the basket to save them, so that you can back to find or analyse them later.,p>,a href=/help/basket target=_top>More...,/a>,/p> ...

The virus, which causes coughing, fever and sometimes fatal pneumonia, has been reported in more than 500 patients, mainly in Saudi Arabia, and has spread to neighboring countries, as well as in a few cases to Europe, Asia and the United States. It kills about 30 percent of those who are infected.

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This immunocytochemical study evaluates the presence of IgG1C4, IgA and IgE immunoglobulins in active lesions of 25 localized cutaneous leishmaniasis patients from three bioclimatic areas (Awa, Afa and Bsha) in Mrida State, Venezuela. regularly in patients through the Awa region than in those through the Bsha area. The predominant expression of isotypes IgG2 and IgG1 suggests a preferential Th1 like immune response. Anti-immunoserum stained just parasites and their particles, suggesting that a lot of from the immunostaining was non-specific. 1989; Islam 1991). Consequently, its been broadly reported how the isotype serum antibodies could be utilized as an sign for Th lymphocyte subset dominance (Finkelman 1990). Histopathological and immunocytochemical research demonstrating the great quantity of plasma cells and IgM, IgG and IgE antibodies complexed to antigens in the infiltrate of human and experimental lesions suggest that the humoral response might influence the elimination of the parasite ...

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King, H.S., & Bell, A.C. (1932). The detection of methanol in the presence of ethyl alcohol. Proceedings of the Nova Scotian Institute of Science, 18(1), 11-13 ...

King, H.S., & Bell, A.C. (1932). The detection of methanol in the presence of ethyl alcohol. Proceedings of the Nova Scotian Institute of Science, 18(1), 11-13 ...

The Bacillaceae are a family of rod-shaped, usually Gram-positive bacteria that produce endospores and are commonly found in spores. Genera commonly is identified as bacillus. The Bacillus are aerobic bacteria, peritrichously flagellated and are either motile or non motile. Most are non-pathogenic; however, the most common pathogen of the species is Bacillus anthracis, which causes anthrax. ...

BspAC I-Source: Bacillus species AC. No nonspecific activity after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Spore-forming bacteria, Bacillus sp., frequently been associated with the contamination of rice and other starchy products. Spores are more resistant to antimicrobial treatments than its vegetative cells. The extract of Indonesian bay leaf (Syzygium polyanthum L.) was assessed for its antibacterial and sporicidal activities against vegetative cells and spores of B. cereus isolated from rice (25 strains). The results showed that S. polyanthum L. extract was able to inhibit the growth of vegetative cells of all B. cereus isolates with MICs ranged from 0.16 to 0.63 mg/mL and can kill with MBCs ranged from 0.31 to 2.50 mg/mL. The bactericidal endpoint for B. cereus BC-NP.8 in time kill curve was at 1.25 mg/mL (8× MIC) after 4 h of incubation while for B. cereus ATCC 33019 was at 2.50 mg/mL (8× MIC). The sporicidal activity of S. polyanthum L. extract was not affected by different temperatures treatment and alteration of the pHs of extract. Therefore, this indicates that the extract was stable ...

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The University of Alberta must maintain control records for all ethyl alcohol purchases. For the University of Alberta to pay lesser rates of duty on purchases, we are obligated to show that the distributions are strictly for educational and research purposes. Regulations from Revenue Canada, Excise Duty Division stipulate that records must be maintained until the alcohol is used. The responsibility is on each department/laboratory to keep accurate usage records and to ensure that all ethyl alcohol is kept in locked storage ...

The physiological effects of ethyl alcohol depend almost entirely on its concentration in the blood stream. This concentration, expressed in per cent, is referred to as the blood alcohol level and has become of increasing medico-legal importance in determining the degree of alcoholic intoxication.

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Bacillus Cereus Bacillus cereus is a rod-shaped gram- positive bacillus that can be found in food, dust, dirt and sometimes soil. It is an aerobe and a

Bacillus cereus type I food poisoning is it contagious? Contagiousness of Bacillus cereus type I food poisoning including infectiousness, transmission, and contagion methods and vectors.

Bacillus cereus ATCC ® 12826™ Designation: Type Strain A, variant IV TypeStrain=False Application: Sporicidal test Bacteriophage host

Avaliação da prevalência de bacillus cereus em leite pasteurizado e comportamento desse microrganismo durante armazenamento sob temperatura crítica de ...

Un factor limitante en la producción de papa es la escasez de tubérculo semilla de papa libre de patógenos; por lo tanto, es necesario encontrar nuevas alternativas de producción de tubérculo semilla en forma eficiente ...

Baldassarre, D. T., T. A. White, J. Karubian and M. S. Webster (2014). Genomic and morphological analysis of a semipermeable avian hybrid zone suggests asymmetrical introgression of a sexual signal. Evolution 68(9): 2644-2657.. Benites, P., L. Campagna and P. L. Tubaro (2014). Song-based species discrimination in a rapid Neotropical radiation of grassland seedeaters. Journal of Avian Biology: n/a-n/a.. Burns, K. J., A. J. Shultz, P. O. Title, N. A. Mason, F. K. Barker, J. Klicka, S. M. Lanyon and I. J. Lovette (2014). Phylogenetics and diversification of tanagers (Passeriformes: Thraupidae), the largest radiation of Neotropical songbirds. Molecular Phylogenetics and Evolution 75: 41-77.. Campagna, L., C. Kopuchian, P. L. Tubaro and S. C. Lougheed (2014). Secondary contact followed by gene flow between divergent mitochondrial lineages of a widespread Neotropical songbird (Zonotrichia capensis). Biological Journal of the Linnean Society 111(4): 863-868.. Dickinson, J. L., E. D. Ferree, C. ...

Bacillus cereus ATCC ® 10987D-5™ Designation: Genomic DNA from Bacillus cereus strain NRS 248 TypeStrain=False Application:

Home testing and Bacillus cereus type I food poisoning, diagnostic tests, self assessment, and other tools and products in relation to Bacillus cereus type I food poisoning.

Morphology of Bacillus cereus. It is gram positive rod shaped bacilli with square ends. It contains spores with central spores and is 1x3-4 µm in size.

216 S11-E2 Page 2 Name Key I. (9 points) Answer in the boxes below the following questions for the Grignard reagent C 3 -Mg. (1) (2 points) Is the carbon atom associated with magnesium electrophilic or

Jan 02, 2020 (The Expresswire) -- Global Bacillus Licheniformis Market is an overview of the global market is delivered with prime focus on...

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Ethanol Ethanol IUPAC name Ethanol Other names Ethyl alcohol; grain alcohol; hydroxyethane; drinking alcohol Identifiers CAS number 64-17-5 RTECS number

When equal volumes of water and ethyl alcohol are mixed, the total volume is less than that of the two liquids before mixing. What happened to the vanishing volume?