Symbiobacterium toebii is a commensal symbiotic thermophile that absolutely requires its partner bacterium Geobacillus toebii for growth. Despite development of an independent cultivation method using cell-free extracts, the growth of Symbiobacterium remains unknown due to our poor understanding of the symbiotic relationship with its partner bacterium. Here, we investigated the interrelationship between these two bacteria for growth of S. toebii using different cell-free extracts of G. toebii. Symbiobacterium toebii growth-supporting factors were constitutively produced through almost all growth phases and under different oxygen tensions in G. toebii, indicating that the factor may be essential components for growth of G. toebii as well as S. toebii. The growing conditions of G. toebii under different oxygen tension dramatically affected to the initial growth of S. toebii and the retarded lag phase was completely shortened by reducing agent, L-cysteine indicating an evidence of commensal interaction of
A Gram-stain-variable, motile, moderately halophilic bacterial strain, CHL-21T, was isolated from a marine solar saltern and its taxonomic position was investigated using a polyphasic approach. Optimal growth of strain CHL-21T occurred at 30-37 °C, at pH 7.0-7.5 and in the presence of 5-10 % (w/v) NaCl. In phylogenetic trees based on 16S rRNA gene sequences, strain CHL-21T fell within the cluster comprising members of the genera Oceanobacillus, Ornithinibacillus and Paucisalibacillus. Strain CHL-21T exhibited 97.1-97.2 % 16S rRNA gene sequence similarity to the type strains of the two subspecies of Oceanobacillus oncorhynchi and 92.0-94.7 % 16S rRNA gene sequence similarity to the type strains of other members of the genus Oceanobacillus and members of the genera Ornithinibacillus and Paucisalibacillus. Mean DNA-DNA reassociation values between strain CHL-21T and the type strains of the two subspecies of Oceanobacillus oncorhynchi were 19-21 %. The cell-wall peptidoglycan of strain CHL-21T was based on
The control of Aedes aegypti is usually based on chemical insecticides, but the overuse of these compounds has led to increased resistance. The binary toxin produced by Lysinibacillus sphaericus in the final stages of sporulation is used for mosquito control due to its specificity against the culicid larvae; however, it has been proved that Ae. aegypti is refractory for this toxin. Currently, there is no evidence of the use of L. sphaericus vegetative cells for mosquito biocontrol. Therefore, in this study, the vegetative cells of three L. sphaericus strains were assessed against a field-collected Ae. aegypti, resistant to temephos, and the reference Rockefeller strain. Vegetative cells of L. sphaericus 2362, III(3)7 and OT4b.25 produced between 90% and 100% of larvae mortality in the reference Rockefeller strain. Effective concentrations of each L. sphaericus strain for the four larval stages ranged from 1.4 to 2 × 107 CFU/ml. Likewise, a consortium of L. sphaericus assessed against a field-collected
Lysinibacillus sphaericus (reclassified - previously known as Bacillus sphaericus) is a Gram-positive, mesophilic, rod-shaped bacterium commonly found on soil. It can form resistant endospores that are tolerant to high temperatures, chemicals and ultraviolet light and can remain viable for long periods of time. It is of particular interest to the World Health Organization due to the larvicide effect of some strains against two mosquitoes genera (Culex and Anopheles), more effective than Bacillus thuringiensis, frequently used as a biological pest control. Its important to highlight that its uneffective against Aedes aegypti, an important vector of yellow fever and dengue viruses. Within the species L. sphaericus it exists five homology groups (I-V), with group II further dividing into subgroups IIA and IIB. Due to the low levels of homology between groups, it has been suggested that each might represent a distinct species, but owing to a lack of research on this topic, all remain designated as ...
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The most distinguishing feature of most members of the family Bacillaceae (phylum Firmicutes) is their ability to form endospores that provide high resistance to heat, radiation, chemicals, and drought, allowing these bacteria to survive adverse conditions for a prolonged period of time. Bacillaceae are widely distributed in natural environments, and their habitats are as varied as the niches humans have thought to sample. Over the years of microbiological research, members of this family have been found in soil, sediment, and air, as well as in unconventional environments such as clean rooms in the Kennedy Space Center, a vaccine-producing company, and even human blood ( 1 - 3 ). Moreover, members of the Bacillaceae have been detected in freshwater and marine ecosystems, in activated sludge, in human and animal systems, and in various foods (including fermented foods), but recently also in extreme environments such as hot solid and liquid systems (compost and hot springs, respectively), salt lakes, and
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p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
We assessed the applicability of bacterial surface layer proteins of Lysinibacillus sphaericus JG-B53 and Sporosarcina ureae ATCC 13881 for the detection of metal ions in water. Based on the interactions of the cell components with metal complexes, two potential sensor systems, one colorimetric with functionalized gold nanoparticles and the other using a regenerative sensor layer, were developed. The systems detection limits of YCl3 in water were 1.67 x 10−5 and 1 x 10−4 mol L−1, respectively ...
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The Bacillaceae are a family of rod-shaped, usually Gram-positive bacteria that produce endospores and are commonly found in spores. Genera commonly is identified as bacillus. The Bacillus are aerobic bacteria, peritrichously flagellated and are either motile or non motile. Most are non-pathogenic; however, the most common pathogen of the species is Bacillus anthracis, which causes anthrax. ...
» The Structure of the Lipid A from the Halophilic Bacterium Spiribacter salinus M19-40T. | TOLLerant - Toll-Like Receptor 4 activation and function in diseases: an integrated chemical-biology approach.
Initial step of β-oxidation is catalyzed by acyl-CoA dehydrogenase in prokaryotes and mitochondria, while acyl-CoA oxidase primarily functions in the peroxisomes of eukaryotes. Oxidase reaction accompanies emission of toxic by-product reactive oxygen molecules including superoxide anion, and superoxide dismutase and catalase activities are essential to detoxify them in the peroxisomes. Although there is an argument about whether primitive life was born and evolved under high temperature conditions, thermophilic archaea apparently share living systems with both bacteria and eukaryotes. We hypothesized that alkane degradation pathways in thermophilic microorganisms could be premature and useful to understand their evolution. An extremely thermophilic and alkane degrading Geobacillus thermoleovorans B23 was previously isolated from a deep subsurface oil reservoir in Japan. In the present study, we identified novel membrane proteins (P16, P21) and superoxide dismutase (P24) whose production levels were
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Arabinanases are glycosidases that hydrolyse α-(1→5)- arabinofuranosidic linkages found in the backbone of the pectic polysaccharide arabinan. Here we describe the biochemical characterization and the enzyme-substrate crystal structure of an inverting family 43 arabinanase from Geobacillus stearothermophilus T-6 (AbnB). Based on viscosity and reducing power measurements, and based on product analysis for the hydrolysis of linear arabinan by AbnB, the enzyme works in an endo mode of action. Isothermal titration calorimetry studies of a catalytic mutant with various arabino-oligosaccharides suggested that the enzyme active site can accommodate at least five arabinose units. The crystal structure of AbnB was determined at 1.06 Å (1 Å=0.1 nm) resolution, revealing a single five-bladed-β-propeller fold domain. Co-crystallization of catalytic mutants of the enzyme with different substrates allowed us to obtain complex structures of AbnBE201A with arabinotriose and AbnBD147A with arabinobiose. ...
Geobacillus stearothermophilus ATCC ® 10149™ Designation: NRS T15 TypeStrain=False Application: Assay of lactam antibiotics beta-lactam antibiotics This strain is recommended by ATCC for use in the tests described in AOAC Official Methods 982.15, 982.18, and 988.08 where only the taxon is specified. Food testing
Geobacillus stearothermophilus ATCC ® 10149™ Designation: NRS T15 TypeStrain=False Application: Assay of lactam antibiotics beta-lactam antibiotics This strain is recommended by ATCC for use in the tests described in AOAC Official Methods 982.15, 982.18, and 988.08 where only the taxon is specified. Food testing
A moderately thermophilic and alkaliphilic bacillus, which had been reported and designated BLx ( Haruta et al., 2002 ), was isolated from a semi-continuous decomposing system of kitchen refuse. Cells of strain BLxT were strictly aerobic, rod-shaped, motile and spore forming. The optimum temperature and pH for growth were approximately 50 °C and pH 8-9. Strain BLxT was able to grow at NaCl concentrations from 0·5 to 7·5 %, with optimum growth at 0·5 % NaCl. The predominant menaquinone was MK-7, and the major fatty acid was iso-C15 : 0. Phylogenetic analysis showed that strain BLxT was positioned in an independent lineage within the cluster that includes the genera Virgibacillus and Lentibacillus in Bacillus rRNA group 1. Strain BLxT exhibited 16S rDNA similarity of 92·8-94·8 % to Virgibacillus species and 92·3 % to Lentibacillus salicampi. Phenotypic, chemotaxonomic and phylogenetic analyses supported the classification of strain BLxT in a novel genus and species. Cerasibacillus quisquiliarum gen
Anaerococcus is a genus of bacteria. Its type species is Anaerococcus prevotii. These bacteria are Gram-positive, anaerobic, indole-negative cocci. Its genome was sequenced in August 2009. It can cause infection and is a normal part of the human microbiome. It is a pathogen of humans found in ovarian abscesses, chronic wounds and vaginal discharge. It can be present in urinary tract infections, chronic ulcers, pleural empyema, blood infections, and soft tissue infections. It is involved in polymicrobial infections. Anaerococcus senegalensis Anaerococcus murdochii Anaerococcus vaginalis Anaerococcus tetradius Anaerococcus prevotii Anaerococcus octavius Anaerococcus lactolyticus Parte, A.C. Anaerococcus. Retrieved 27 March 2017. BacMap. Retrieved 27 March 2017. Murphy, Elizabeth Carmel; Frick, Inga-Maria (2013). Gram-positive anaerobic cocci - commensals and opportunistic pathogens. FEMS Microbiology Reviews. 37 (4): 520-553. doi:10.1111/1574-6976.12005. ISSN 1574-6976 ...
1. Buchanan, R.E., and Gibbons, N.E., Eds. (1974) Bergeys Manual of Determinative Bacteriology, 8th ed., Williams and Wilkins Co., Baltimore, Md. 2. Claus, D., Fritze, D., Kocur, M., (2006) Genera related to the genus Bacillus-Sporolactobacillus, Sporosarcina, Planococcus, Filibacter and Caryophanon. The Prokaryotes , Vol. 4, 3rd edn (Dworkin M Falkow S Rosenberg E Schleifer KH Stackebrandt E, eds), pp. 631-653. Springer, New York, NY. 3. Nucleotide [Internet]. Bethesda (MD): National Library of Medicine (US), National Center for Biotechnology Information; [2017]. Accession No. NZ_CP015109.1, Sporosarcina ureae strain P17a, complete genome. [cited 2018 April, 14]. Available from: https://www.ncbi.nlm.nih.gov/nuccore/NZ_CP015109.1 4. Chary, V. K. & Piggot, P. J. Postdivisional synthesis of the Sporosarcina ureae DNA translocase SpoIIIE either in the mother cell or in the prespore enables Bacillus subtilis to translocate DNA from the mother cell to the prespore. J. Bacteriol. 185, 879-886 (2003). ...
The gene encoding the GK0453 protein (gi:56418988) was amplified via PCR using G. kaustophilus HTA426 genomic DNA and was cloned into the pET-15b expression vector (Merck Novagen, Darmstadt, Germany). The tobacco etch virus (TEV) protease recognition sequence was inserted in the N-terminal tag region of the expression vector, which was then introduced into the Escherichia coli Rosetta (DE3) strain (Merck Novagen, Darmstadt, Germany). The recombinant strain was cultured in 5 l LB medium containing 30 µg ml−1 chloramphenicol and 50 µg ml−1 ampicillin. The harvested cells (23.3 g) were lysed by sonication on ice in 35 ml of 20 mM Tris-HCl buffer pH 8.0 containing 500 mM NaCl, 5 mM β-mercaptoethanol and 1 mM phenylmethylsulfonyl fluoride. The cell lysate was heat-treated at 343 K for 13 min and then centrifuged at 15 000g for 30 min at 277 K. The supernatant was applied onto a HisTrap HP column (GE Healthcare Biosciences) equilibrated with 20 mM Tris-HCl buffer pH 8.0 containing 500 mM NaCl ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Dive into the research topics of Draft genome sequence of Geobacillus sp. LEMMY01, a thermophilic bacterium isolated from the site of a burning grass pile. Together they form a unique fingerprint. ...
Sigma-Aldrich offers abstracts and full-text articles by [J W Swearingen, D E Fuentes, M A Araya, M F Plishker, C P Saavedra, T G Chasteen, C C Vásquez].
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
General Information: Anoxybacillus flavithermus, formerly Bacillus flavothermus, was isolated from a hot spring in New Zealand. This organism can tolerate a pH of up to 9.0. Gram-positive bacteria of the genus Anoxybacillus have been found in diverse thermophilic habitats, such as geothermal hot springs and manure, and in processed foods such as gelatin and milk powder. ...
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S. ureae is a motile, Gram-positive sporeforming bacteria which is coccoid in shape,[1] its being a coccus is somewhat surprising given its close relation to the Bacillus genus, members of which are rod-shaped. S. ureae is distributed across the globe and is typically found in fertile soils and is especially prevalent in those containing high levels of urea. Its ability to grow in the presence high amounts of urea is due to its ability to convert urea in soil to ammonia through the production of urease.[2] The ability for S. ureae to covert urea to ammonia offers a promising means by which to decrease fertilizer use in agricultural areas. This could be accomplished by rotating a plot of land from being an animal pasture, in which large amounts of urea would be deposited, and then being converted into a field the next year, at which time S. ureae would have converted the urea into ammonia for the new plants to use. Despite their close relationship and numerous physical similarities, the unique ...
SWISS-MODEL Template Library (SMTL) entry for 2egg.1. Crystal Structure of Shikimate 5-Dehydrogenase (AroE) from Geobacillus kaustophilus
isolates originating from thermal springs water of Zara and Main in Jordan. The isolate was as spore-forming bacilli. Species was chosen for further studies due to its ability to grow in minimal salt media and high ability to secrete extracellular protease enzymes during growth on suitable media such as skimmed milk.. Morphological studies conducted on the strain showed interesting observations that can be summarized as follows:. The bacterial isolate WI is rod shaped, and motile by peritrichous flagella (fig 1).. The formation of spheroplasts seen in the isolated cluster is an important characteristic during the decline phase of growth.. The chemotaxonomic methods employed did not allow further differentiation below the level of the genus.. The new isolate is aerobic catalase-producing microorganism, distinguished by the formation of terminal cylindrical endospore, characterizing members of the family Bacillaceae.. The WI has great ability to ferment different types of sugars and carbon ...
SWISS-MODEL Repository entry for P94456 (KDPA_GEOSE), Potassium-transporting ATPase potassium-binding subunit. Geobacillus stearothermophilus (Bacillus stearothermophilus)
Lineage: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; Bacillus ...
Lineage: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; group; Bacillus ...
A new high-alkaline protease (ALTP) was purified to homogeneity from a culture of the strictly anaerobic and extremely alkaliphilic Alkaliphilus transvaale
A keratinolytic proteinase was cloned from thermophilic bacterium Geobacillus stearothermophilus AD-11 and was expressed in Escherichia coli BL21(DE3). Recombinant keratinolytic proteinase (RecGEOker) with an estimated molecular weight of 57 kDa was purified and keratinase activity was measured. RecGEOker showed optimal activity at pH 9 and 60 °C. Recombinant keratinolytic proteinase showed the highest substrate specificity toward keratin from wool , collagen , sodium caseinate , gelatin , and BSA in descending order. RecGEOker is applicable for efficient keratin waste biodegradation and can replace conventional non-biological hydrolysis processes. High-value small peptides obtained from enzymatic biodegradation by RecGEOker are suitable for industrial application in white and/or green biotechnology for use as major additives in various products. ...
Author. By Mogahid M. El Hassan, Kanury V.S. Rao, Zaved Siddique, Dinesh S. Kumar, Rashmi Tickoo, Nageeb S. Saeed, Moawia M. Mukhtar, Mohamed E. Hamid .. Abstracts. Nocardia africana is a pulmonary pathogen which has been recently isolated from human in Sudan. There is great need for proteomic investigations but they are sparse at present. First attempts on N. africana SD 769 (DSM44491) visualized 74 protein of molecular weights between 10 -250 kDa and isoelectric points between 4 7. Due to the limited database information, identified proteins were assigned by homology to sequences from the other bacterial species such as Staphylococcus aureus, Oceanobacillus iheyensis, N. farcinica and Yersinia pestis References. FULL Text ...
A thermostable extracellular lipase of Geobacillus sp. strain T1 was cloned in a prokaryotic system. Sequence analysis revealed an open reading frame of 1,251 bp in length which codes for a polypeptide of 416 amino acid residues. The polypeptide was composed of a signal peptide (28 amino acids) and a mature protein of 388 amino acids. Instead of Gly, Ala was substituted as the first residue of the conserved pentapeptide Gly-X-Ser-X-Gly. Successful gene expression was obtained with pBAD, pRSET, pET, and pGEX as under the control of araBAD, T7, T7 lac, and tac promoters, respectively. Among them, pGEX had a specific activity of 30.19 Umg−1 which corresponds to 2927.15 Ug−1 of wet cells after optimization. The recombinant lipase had an optimum temperature and pH of 65°C and pH 9, respectively. It was stable up to 65°C at pH 7 and active over a wide pH range (pH 6-11). This study presents a rapid cloning and overexpression, aimed at improving the enzyme yield for successful industrial ...
The Bacillus cereus sensu lato group consists of six species (B. anthracis, B. cereus, B. mycoides, B. pseudomycoides, B. thuringiensis, and B. weihenstephanensis). While classical microbial taxonomy proposed these organisms as distinct species, newer molecular phylogenies and comparative genome sequencing suggests that these organisms should be classified as a single species (thus, we will refer to these organisms collectively as the Bc species-group). How do we account for the underlying similarity of these phenotypically diverse microbes? It has been established for some time that the most rapidly evolving and evolutionarily flexible portions of the bacterial genome are regulatory sequences and transcriptional networks. Other studies have suggested that the sigma factor gene family of these organisms has diverged and expanded significantly relative to their ancestors; sigma factors are those portions of the bacterial transcriptional apparatus that control RNA polymerase recognition for promoter
This is a homepage of the National Institute of Technology and Evaluation (NITE).Information on Brevibacillus brevis NBRC 100599 (= 47) of Biotechnology Field is published.
ID A0A0F6BI10_GEOS0 Unreviewed; 115 AA. AC A0A0F6BI10; DT 24-JUN-2015, integrated into UniProtKB/TrEMBL. DT 24-JUN-2015, sequence version 1. DT 11-DEC-2019, entry version 22. DE RecName: Full=DUF3870 domain-containing protein {ECO:0000259,Pfam:PF12986}; GN OrderedLocusNames=GY4MC1_0212 {ECO:0000313,EMBL:ADP73061.1}; OS Geobacillus sp. (strain Y4.1MC1). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; OC unclassified Geobacillus. OX NCBI_TaxID=581103 {ECO:0000313,EMBL:ADP73061.1}; RN [1] {ECO:0000313,EMBL:ADP73061.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Y4.1MC1 {ECO:0000313,EMBL:ADP73061.1}; RG US DOE Joint Genome Institute; RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., RA Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R., Land M., RA Hauser L., Jeffries C., Kyrpides N., Ivanova N., Ovchinnikova G., Brumm P., RA Mead D., Woyke T.; RT Complete sequence of chromosome of Geobacillus sp. Y4.1MC1.; RL Submitted ...
Halophilic bacteria grow over a wide range of salt concentrations. In this study we aimed to isolate and screen out the halophilic bacteria and to determine their activity for production of the bioactive compounds. A total of 50 water, sediments and soil samples were collected from Maharlu salt lake in southern region of Fars-Iran and subjected for isolation of the bioactive compound producing Halophilic bacteria. The results obtained indicated that out of all isolates, three strains could produce the bioactive compounds. The isolates were molecular (16SrRNA) identified as Bacillus licheniformis, Bacillus subtilis and Bacterium Culaeen. Furthermore, structural analysis of the bioactive compounds was carried out in order to achieve maximum information concerning to them. The results obtained illustrated the existence of glycoprotein in all the bioactive compounds. Although, Staphylococcus aureus, Aspergillusniger and Mucor…. were sensitive to all the bioactive compounds, Pseudomonas aeruginosa,
ID A0A0F6BHF6_GEOS0 Unreviewed; 450 AA. AC A0A0F6BHF6; DT 24-JUN-2015, integrated into UniProtKB/TrEMBL. DT 24-JUN-2015, sequence version 1. DT 11-DEC-2019, entry version 34. DE RecName: Full=Chromosomal replication initiator protein DnaA {ECO:0000256,HAMAP-Rule:MF_00377, ECO:0000256,RuleBase:RU000577, ECO:0000256,SAAS:SAAS01081161}; GN Name=dnaA {ECO:0000256,HAMAP-Rule:MF_00377}; GN OrderedLocusNames=GY4MC1_0001 {ECO:0000313,EMBL:ADP72857.1}; OS Geobacillus sp. (strain Y4.1MC1). OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus; OC unclassified Geobacillus. OX NCBI_TaxID=581103 {ECO:0000313,EMBL:ADP72857.1}; RN [1] {ECO:0000313,EMBL:ADP72857.1} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=Y4.1MC1 {ECO:0000313,EMBL:ADP72857.1}; RG US DOE Joint Genome Institute; RA Lucas S., Copeland A., Lapidus A., Cheng J.-F., Bruce D., Goodwin L., RA Pitluck S., Chertkov O., Zhang X., Detter J.C., Han C., Tapia R., Land M., RA Hauser L., Jeffries C., Kyrpides N., Ivanova N., ...
YAO, Haiyan; REN, Yuan; WEI, Chaohai y YUE, Siyang. Biodegradation characterisation and kinetics of m-cresol by Lysinibacillus cresolivorans. Water SA [online]. 2011, vol.37, n.1, pp.15-20. ISSN 1816-7950.. A novel strain of m-cresol-degrading bacterium, named as Lysinibacillus cresolivorans, was isolated from aerobic sludge from a coking wastewater treatment plant. This bacterium is able to utilise m-cresol as its sole source of carbon and energy. The optimal pH for growth is 6.8 ~ 7.3 and the optimal temperature is 35ºC. Compared to organic nitrogen sources, inorganic nitrogen sources were easily utilised for the m-cresol biodegradation. The degradation rate of m-cresol at different starting concentrations was analysed with zero-order kinetic characteristics. When the initial concentration of m-cresol was 224.2 mg·ℓ-1, the reaction rate reached a maximum at 46.80 mg·(ℓ·h)-1.The cell growth kinetics was also investigated with initial m-cresol concentrations varying from 0 to 1 200 ...
Abstract: The crystal structure has been determined of the F1-catalytic domain of the ATP synthase from Mycobacterium smegmatis which hydrolyzes adenosine triphosphate (ATP) very poorly. The structure of the α3β3-component of the catalytic domain is similar to those in active F1-ATPases in Escherichia coli and Geobacillus stearothermophilus. However, its ε-subunit differs from those in these two active bacterial F1-ATPases as an ATP molecule is not bound to the two α-helices forming its C-terminal domain, probably because they are shorter than those in active enzymes and they lack an amino acid that contributes to the ATP binding site in active enzymes. In E. coli and G. stearothermophilus, the α-helices adopt an up state where the α-helices enter the α3β3-domain and prevent the rotor from turning. The mycobacterial F1-ATPase is most similar to the F1-ATPase from Caldalkalibacillus thermarum, which also hydrolyzes ATP poorly. The βE-subunits in both enzymes are in the usual open ...
Isolation of hydrocarbon-degrading strain and culture condition. Approximately 2 g crude oil sample, collected from the Liaohe oilfield in the northeast of China, was suspended in 50 ml sterile water containing 0.15 g Tween-80 and 10 glass beads in diameter of 0.5 cm and shaken at 60°C and 120 rev/min. After the crude oil was completely emulsified, an aliquot of 10 ml emulsified crude oil was inoculated into 100 ml nutrient broth and incubated at 60°C and 120 rev/min for 2 days. The liquid culture was transferred into fresh nutrient broth at 10% inoculum and incubated in the same conditions for another 2 days. After five cycles of such transformation and incubation for bacterial enrichment, a 10-fold serial dilution of the enriched culture with sterile saline was spread on the selective medium plates. All plates were incubated at 60°C for 48 h. Colonies with different colony characteristics were selected from the selective medium plates and incubated in 100 ml of the liquid selective medium ...
With a very low D-Value data for the gold standard medical grade spores of Geobacillus stearothermophilus of 2.3 - 2.6 D-Value (meaning a 90% kill within only 2.3 to 2.6 minutes of treatment), it wont take long to high level disinfect surfaces, including complex surface geometries, even in large process areas. Altapures system is easily scalable for any application or treatment area both large or small.. Altapures high level disinfection products is able to rapidly achieve clinically proven No Growth results on ALL treated room surfaces tested for: Bacterial Spores, Virus, & Vegetative Bacteria.. In addition, Altapures submicron sized droplets provide a gas-like diffusion of the treatment agent throughout the room, where the Altacare ™ cold sterilant is deposited as an extremely thin film.. The result is a touch free / no touch high level disinfection system that is able to reach no growth cultures on ALL treated surfaces, even in large rooms, for bacteria such as but not limited ...
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
Six Log Solutions are the most cost effective provider of hydrogen peroxide decontamination services in Ireland. We guarantee the shortest downtime and thorough efficiency of our iHP process which is validated by use of 6-log biological indicators of geobacillus stearothermophilus.. We are unique in that we have iHP processes suitable for a wide range of sectors. For healthcare applications we provide a decontamination solution that leaves behind a silver ion barrier to protect against micro-biological regrowth. Alternatively for pharmaceutical applications, we use a silver-free solution that meets the requirements of this industry.. Since our launch in Ireland, we have successfully bio-decontaminated hundreds of rooms across a wide range of industries.. ...
2.0 2.1 Bisby F.A., Roskov Y.R., Orrell T.M., Nicolson D., Paglinawan L.E., Bailly N., Kirk P.M., Bourgoin T., Baillargeon G., Ouvrard D. (red.) (2011). Species 2000 & ITIS Catalogue of Life: 2011 Annual Checklist.. Species 2000: Reading, UK. Ginkuhà 24 september 2012. ...