HPRS-103, the prototype of avian leukosis virus (ALV) subgroup J, was isolated in 1989 from meat-type chickens from commercial flocks where it induces myelocytic myeloid leukosis (ML). The HPRS-103 env gene differs considerably from other ALV subgroups but shows high identity (75-97%) to env-like sequences of the different members of the EAV family of endogenous avian retroviruses. Recently, we have isolated several viruses related to HPRS-103 from cases of ML. Although these isolates showed properties of ALV subgroup J, the majority of them resisted neutralization by HPRS-103-specific serum, suggesting antigenic variation. The nucleotide sequence of the env gene of the variant viruses showed several substitutions resulting in amino acid changes especially clustered in the variable regions hr1, hr2 and vr3. Analysis of the data suggests that selection pressure, probably from the immune response, is driving the antigenic variation among the isolates. Phylogenetic analysis of the sequences showed the
The host ranges of the Rous sarcoma virus (RSV) pseudotype RSV(HPRS-103) of a novel avian leukosis virus (ALV), strain HPRS-103, and representative RSV pseudotypes of subgroups A to F, have been determined in embryo fibroblasts from 12 avian species. Domestic fowl, red jungle fowl, Sonnerat's jungle fowl and turkey were susceptible to infection by RSV(HPRS-103); ring-necked pheasant, Japanese green pheasant, golden pheasant, Japanese quail, guinea-fowl, Peking duck, Muscovy duck and goose were resistant. The host range pattern of RSV(HPRS-103) differs from those of viruses of subgroups A to G and I, and provides support for placing the HPRS-103 strain of ALV in a new envelope subgroup, designated J.
We have previously described avian leukosis virus-based packaging cell lines that express gag, pol, and env proteins from two transcomplementing genomes and produce helper-free stocks of retroviral vectors with different host ranges. In this report, we demonstrated that (i) despite the deletion of the psi packaging sequence, the packaging-defective transcomplementing retroviral transcripts were packaged into virions at a level that could reach 2.3% of a wild-type virus packaging level and (ii) despite deletion of the 3 LTR, these genomes were transferred along with the vector to target cells. As these genomes were also bearing a selectable gene, titers of the resulting contaminant particles could be estimated, depending on the cell line to be between 0 and 6 infectious particles/ml of supernatant.
Traditionally, virus isolates have been named most commonly for the disease from which they were isolated - e.g. influenza, poliomyelitis, visna, foot-and-mouth disease, and the like. A smaller number are named for the place of isolation - Coxsackie, Sindbis, and Sendai viruses are a few examples, and a mercifully small number are named for their discoverers - alone or in combination with other features - such as Epstein-Barr virus, Rous sarcoma virus, etc. With retroviruses, it is also usual to append the species of origin to the name - avian leukosis virus, human T-cell lymphoma virus, baboon endogenous virus, etc. Unlike most other organisms, the naming of new virus isolates has usually been a trivial prerogative of the isolator, and not subject to review by other workers in the field . This has, in general, caused little difficulty so long as the names chosen were novel and did not presuppose unproven relationships. Significant confusion has been created in the past, however, by injudicious ...
Induction of lymphomas in the bursa of Fabricius of chickens by replication competent, non-transforming avian retroviruses (Avian Leukosis Virus, ALV) is both an economically significant, naturally...
Compare E26 avian leukemia oncogene 2, 3 domain Biomolecules from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.
A team led by Xin Li, Ph.D., assistant professor in the departments of Biochemistry and Biophysics and Urology at the University of Rochester School of Medicine and Dentistry, analyzed rooster testes to find out.. Chickens acquire and harbor a wide variety of viruses. When a virus infects a host, like a chicken, it does everything it can to survive. One method of survival is inserting its genetic material into the chickens genome. Over generations, the inserted virus accumulates mutations and eventually becomes harmless to the animal, but its still a part of the chickens genetic material.. Lis team focused on avian leukosis virus, which commonly infects and can lead to cancer in domestic chickens. Through molecular and genetic analysis, they discovered that chickens turn these old, existing viruses into piRNA-producing machines. When faced with a new avian leucosis virus (there are many different viruses in the family), the old viruses pump out piRNAs that defend the germ cells, ensuring the ...
The avian acute leukemia virus (E26) induces a mixed erythroidmyeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. The viral protein responsible for transformation is a gag-myb-ets fusion protein that is located in the nucleus of the transformed cells. The cellular homologue of v-ets (c-ets-1) is highly expressed in lymphoid cells and differs from the v-ets gene at its carboxy terminal region. Here, we show that both the c-ets-1 and v-ets gene products are DNA-binding proteins and their DNA-binding activity is located in the carboxy terminal (46 amino acid residues) region. It appears that this DNA-binding activity is modulated by the extreme carboxy terminal region. The amino acid sequences of the putative ets DNA-binding domain at its carboxy terminal region showed a helix-turn-helix secondary structure. Exchanging the nonhomologous extreme carboxy terminal regions of c-ets-1 with v-ets gene sequences showed differences in DNA-binding affinity, indicating that ...
ETS1 (phospho Thr38) antibody (E26 avian leukemia oncogene 1, 5 domain) for ICC/IF, IHC-P, WB. Anti-ETS1 (phospho Thr38) pAb (GTX55307) is tested in Human, Mouse samples. 100% Ab-Assurance.
... the new chromosomal fragment do- nated to a merozygote (q.v.). exogenous DNA DNA that originates outside an organism (e.g., from another cell or virus). exogenous virus a virus ...
used to derive intake values did not include consideration for other applications. However, with the increasing globalization of information and the identification of a variety of factors specific to different population subgroups (e.g ...
/PRNewswire/ -- Autoliv, Inc., the worldwide leader in automotive safety systems, (NYSE: ALV and SSE: ALIVsdb) today announced the results of its annual...
Incidence of avian leukosis virus infection in broiler stocks and its effect on early growth.: Twenty-eight broiler breeder flocks were tested for avian leukosi
Subgroups A, B, E and J are the major subgroups of avian leukosis virus (ALV) infecting chickens. ALV infection has become endemic in China and has a significant negative effect on the poultry industry. Consequently, there is an urgent need for a specific, sensitive and rapid method for diagnosis and eradication of ALV. Therefore, we developed a simple and rapid real-time loop-mediated isothermal amplification (LAMP) reaction for the timely detection of the common ALV subgroups, whereby the amplification can be obtained in 35 min under isothermal conditions at 63 °C, ability to specific, sensitive and rapid detect all the common ALV subgroups. A set of four specific primers was designed to target the sequences of the pol gene of ALV, and the loop-mediated isothermal amplification (LAMP) assay were developed and compared with PCR and virus isolation methods. The results from specificity of the LAMP assay showed that only target ALVs DNA was amplified. The LAMP assay demonstrated a sensitivity of 20
Chicken endogenous viruses, ALVE (Avian Leukosis Virus subgroup E), are inherited as LTR (long terminal repeat) retrotransposons, which are negatively correlated with disease resistance, and any changes in DNA methylation may contribute to the susceptibility to neoplastic disease. The relationship between ALVE methylation status and neoplastic disease in the chicken is undefined. White Leghorn inbred lines 72 and 63 at the ADOL have been respectively selected for resistance and susceptibility to tumors that are induced by avian viruses. In this study, the DNA methylation patterns of 3∼6 CpG sites of four conserved regions in ALVE, including one unique region in ALVE1, the promoter region in the TVB (tumor virus receptor of ALV subgroup B, D and E) locus, were analyzed in the two lines using pyrosequencing methods in four tissues, i.e., liver, spleen, blood and hypothalamus. A significant CpG hypermethylation level was seen in line 72 in all four tissues, e.g., 91.86±1.63% for ALVE region2 in blood,
Morbidity is low but mortality high. Mortality tends to be chronically higher than normal for a prolonged period. Egg production is somewhat reduced. There may be increased susceptibility to other infectious diseases due to damage to the immune system. Vertical transmission is most important by infection of the egg white in infected breeders (who are long-term carriers), lateral transmission is poor but infection may occur by the faecal-oral route, especially in young birds. In lymphoid leukosis the incubation period is about 4-6 months; it may be as short as 6 weeks for some of the other manifestations. The causative viruses are rapidly inactivated at ambient temperature and on exposure to most disinfectants ...
Avian leukosis virus (ALV)-induced osteopetrosis is a proliferative disorder of the bone affecting the growth and differentiation of osteoblasts. Osteopetrosis is a polyclonal disease in which cells of the bone contain, on average, multiple viral DNA copies. Osteopetrotic bone is also characterized by the accumulation of unintegrated viral DNA, suggesting an atypical life cycle of the virus in the infected osteoblasts. To better understand virus-host interactions in the induction of osteopetrosis by ALVs, infected chick osteoblast cultures and osteopetrotic bone were examined for aspects of the virus life cycle and effects of infection on osteoblast function. Levels of infection and virus expression were compared in cultured osteoblasts and osteopetrotic bone. Osteopetrotic bone contained higher levels of viral DNA and correspondingly higher levels of viral proteins than infected osteoblast cultures, suggesting a higher viral load in the diseased bone. A significant level of mature Gag protein was
In addition to neoplasias caused in chickens by helper viruses of the avian myeloblastosis virus (AMV) complex, acute myeloblastic leukemia is induced by a defective leukemogenic component. To...
Avian myeloblastosis ATCC ® VR-1542AS-Gt™ Designation: antiserum against AMV RT [NCI HE 599] Application: goat antiserum against the Avian Myeloblastosis Virus (AMV) Reverse Transcriptase (RT)
TY - JOUR. T1 - Disease tropism of c-erbB. T2 - Effects of carboxyl-terminal tyrosine and internal mutations on tissue-specific transformation. AU - Pelley, R. J.. AU - Maihle, Nita Jane. AU - Boerkoel, C.. AU - Shu, H. K.. AU - Carter, T. H.. AU - Moscovici, C.. AU - Kung, H. J.. PY - 1989/1/1. Y1 - 1989/1/1. N2 - Avian leukosis virus induces erythroleukemia in chickens by proviral insertional mutation of the proto-oncogene c-erbB. The product of the insertionally activated c-erbB locus lacks the extracellular ligand-binding domain and is strictly leukemogenic. It has previously been demonstrated that the disease spectrum associated with aberrant c-erbB expression can be expanded by structural perturbation of the cytoplasmic domain of this protein. In this report, we use mutagenesis and retroviral vectors to identify specific mutations in the carboxyl-terminal domain of the insertionally activated c-erbB product that are sufficient to activate the sarcomagenic potential of this protein. ...
Its another beautiful day on the Old McDonald|farm, and having fed and watered the horses, cows, sheep, and other assorted barnyard animals, you decide...
anti-V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) (pThr358), (AA 340-385) antibody (Cy5.5) ABIN752598 from antibodies-online
Primers 1 and 2 were designed from the mRNA of a guinea pig BK B2 receptor (GenBank accession no. AJ003243) to amplify a 500-base pair fragment. Primers 3 and 4 were a pair of degenerate primers used to amplify a 401-base pair fragment for the guinea pig BK B1 receptor. Primers 3 and 4 were made from the conserved region between mouse BK B1 receptor (GenBank accession no. NM_007539) and rat BK B1 receptor (GenBank accession no. U66107). Amplification was done with an iCycler (Bio-Rad, Hercules, CA). PCR cycles consisted of denaturation for 1 min at 94°C, annealing at 56°C for 90 s, and extension at 72°C for 90 s. A total of 30 cycles was followed by completion of extension for 7 min at 72°C. PCR product (12 μl) was analyzed by electrophoresis on 2% agarose gel. Sequencing was done with an ABI 373XL DNA sequencer after the PCR product was purified with Qiaquick PCR purification kit (QIAGEN, Valencia, CA). RT-PCR without avian myeloblastosis virus reverse transcriptase was used as a negative ...
RT Components: 1U/mL RNase inhibitor, 0.625U/mL reverse transcriptase (avian myeloblastosis virus, Boehringer), 1microM G1 primer, 1.75mM dNTPs ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
The KOMP Repository is located at the University of California Davis and Childrens Hospital Oakland Research Institute. Question? Comments? For Mice, Cells, and germplasm please contact us at [email protected], US 1-888-KOMP-MICE or International +1-530-752-KOMP, or for vectors [email protected] or +1-510-450-7917 ...
In reply to your comment, or should I say link since you did not add any thoughts on the matter I would like to say that "HIV" was observed in laboratory test under artificial stress and its appearance was endogenous. There is to date no picture of this virus observed by electron microscopy in the blood of a patient whose said they have a "high viral load" . More HIV is not an exogenous virus as people who make these claims seem to suggest , but a retrovirus, it is an important difference because our DNA contains 1% of endogenous retroviruses that may under certain conditions trigger diseases ... especially as those found in AIDS. But the question is , what makes the sick cell , why are these scientists able to identify this retro virus in a test tube , but never in humans ...
Hello,. Sometime because of large data or output users set the program to execute in background process.. Once the background job is get completed, they execute the SM37 T-code to view the information, but that information is in list view,. their they does not an option like, sorting, filtering and all.. Its a program, where user are getting the same functionality, but the output will display in ALV format, there they will get the all necessary option to format the data.. Basically the program is created based on the Z* programs, so it will be execute in only for the ALV grid output data.. ...
Indicator values are prone to statistical error (the difference between an estimated value and the true value). The statistical error associated with an indicator depends on the population subgroup (e.g. the population of a county or LGD) that it refers to. Such differences in levels of statistical error can distort what we see in maps and charts. They can make some relationships involving indicators and attributes appear "real" (practically meaningful or statistically significant) when they are in fact spurious; other relationships that are "real" can be masked. These differences in statistical error can even distort the shape of plots or the colour patterns we see in maps. For example, ...
Indicator values are prone to statistical error (the difference between an estimated value and the true value). The statistical error associated with an indicator depends on the population subgroup (e.g. the population of a county or LGD) that it refers to. Such differences in levels of statistical error can distort what we see in maps and charts. They can make some relationships involving indicators and attributes appear "real" (practically meaningful or statistically significant) when they are in fact spurious; other relationships that are "real" can be masked. These differences in statistical error can even distort the shape of plots or the colour patterns we see in maps. For example, ...
Bovine Leukosis is a disease caused by the Bovine Leukosis virus (BLV). This is a blood borne disease that dwells in the lymphocytes (white blood cells) in cattle. Only 5% of infected animals will exhibit the clinical symptoms of developing tumors in lymphatic tissue. The prevalence ofleukosis is wide spread. It is fairly common to find herds with a prevalence of 80-90%, however not very common to find herds that are completely free of leukosis. According to the NAHMS 96 study, 88.5% of dairy herds and 38.7% of beef herds are infected. Transmission occurs by the transfer of bodily fluids that contain infected white cells (blood, colostrum, milk). Anything from reusing without sanitizing contaminated needles, syringes, breeding sleeves, dehorners, or balling guns can transmit leukosis. Transmission can also occur through colostrum or to a fetus in utero. ...
Rosok, Mae Joanne, "Dissociation and isolation of the subunits of avian myeloblastosis virus RNA-directed DNA polymerase" (1977). Graduate Student Theses, Dissertations, & Professional Papers. 6828 ...
hypothetical protein, transcription factor protein, A306_09622, anon-WO03040301.171, avian myelocytomatosis viral oncogene homolog, avian myelocytomatosis viral (v-myc) oncogene homolog, bHLHe39, bHLHe57, cellular myelocytomatosis oncogene, CG10798 gene product from transcript CG10798-RB, CG10798-PA, CG10798-PB, class E basic helix-loop-helix protein 39, cmyc, c-MYC, c-myc20, c-Myc-a, c-Myc-b, c-myc-like protein, c-myc proto-oncogene, diminuitive, diminutive, DM, dm/dMyc, Dmel_CG10798, dm/myc, d-myc, dMYC, dmyc1, EG:BACN5I9.1, EGK_19271, lethal (1) G0354, lethal (1) G0359, MDA_GLEAN10014535, mMyc, MRTL, Myc2, myc-a, myc-b, MYCC, Myc-PA, Myc-PB, myc proto-oncogene protein, myc-related translation/localization regulatory factor, myelocytomatosis oncogene, myelocytomatosis viral oncogene homolog, N303_07797, N307_12984, N309_06569, Niard, Nird, oncoprotein myc, PAL_GLEAN10003365, Proto-oncogene c-Myc, RNCMYC, transcription factor Myc, transcription factor p64, TREES_T100017263, uncharacterized ...
The avian myeloblastosis virus (AMV) is an alpha retrovirus responsible for acute myeloblastic leukemia (AML) when injected in ovo, or in newly hatched chickens [10]. Early in vitro dose response experiments indicated that the production of virion with leukemogenic potential required a double infection with AMV and a helper virus [11]. The AMV strains that are used and commercially available, are derived from the orignal BAI strain A purified from chicken leukemic plasma [12]. Leukemic plasma containing the BAI strain has been widely distributed for many years by Life Sciences Inc., in Florida which has been the official provider of national agencies in the USA. The Standard AMV-S BAI strain is a complex mixture of viruses that also includes two helper viruses in addition to AMV. The helper viruses Myeoloblastosis Associated Virus (MAV) contained in AMV-S belong to two different serological subgoups (type1 and type2, also called A and B). Both of them are oncogenic [13].. Both MAV-1 and MAV-2 ...
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat V-Myc Myelocytomatosis Viral Oncogene Homolog (MYC) in samples from Serum, plasma, tissue homogenates, cell lysates and other biological fluids. with no significant corss-reactivity with analogues from other species ...
ERG antibody, Internal (v-ets avian erythroblastosis virus E26 oncogene homolog) for WB. Anti-ERG pAb (GTX77743) is tested in Human samples. 100% Ab-Assurance.
A testi és szellemi egészség szempontjából fontos a megfelelő éjszakai alvás. Az óra segítségével nyomon követheti az alvását, és figyelheti, hogy átlagosan hány órát alszik.. Ha az órát alvás közben is viseli, a Suunto Spartan Trainer Wrist HR a gyorsulásmérő adatai alapján nyomon követi az alvását. Amikor lefekszik, az órát leteheti az ágy mellé, de javasoljuk, hogy hagyja a karján, mivel így pontosabb adatokat kap az alvásáról.. Az alvás nyomon követéséhez: ...
Glynn, J P.; Halpern, B L.; and Fefer, A, "An immunochemotherapeutic system for the treatment of a transplanted moloney virus-induced lymphoma in mice." (1969). Subject Strain Bibliography 1969. 913 ...
Terms Related to the Moving Wall Fixed walls: Journals with no new volumes being added to the archive. During an 8-year period, we retrospectively collected data on all travelers hospitalized in our department for a CMI occurring during travel or in the month after their return. During an 8‐year period, we retrospectively collected data on all travelers hospitalized in our department for a CMI occurring during travel or in the month after their return. The sensitivity and versatility of this technique (immunofixation can be applied to the detection of any antigen) should permit its extension to other biological fluids with a low protein content. Exogenous viruses including bacteriophage and human herpes viruses-4, -5 and -6 were detected variably in autopsied brains from both clinical groups. This case highlights the challenges of early recognition, accurate diagnosis, appropriate treatment and follow-up of such patients. A small number of children have CP as the result of brain damage in the ...
n miben szokott aludni? T len pizsam ban, ny ron h l ingben vagy als nadr gban? B rmelyik legyen is a v lasz, tudnia kell, hogy rosszul teszi. me hat meglep ok, ami rt rdemes lenne meztelen l aludnia.
Здравсвуйте пользователи форума amv.at.ua, вчера пользователь под ником Olechkalype просила меня выложить супермодные новинки Компьютерные аксессуары/Графические планшеты в 2014, а так же сотовые бренда Intuos Pro Special Ed., так вот наконецто нашла время для этого, админы прошу не удалять, а перенести в соответствующий раздел, если я ошиблась например Компьютерные аксессуары/Графические планшеты ))) всех обнимаю ...
Пожалуй, самым разочаровывающим итогом 2013 года стало сползание в экономический застой. На протяжении года снижались как темпы роста промышленности, так и ВВП. Если в начале года еще казалось, что экономика вот-вот разгонится и из состояния «технической рецессии» войдет в фазу роста, то уже весной тональность заявлений финансовых властей смен ...
Vendredi 22 mars 2013 à 11h30 , Amélie Peres, ENS Paris. Titre : Etude de lévolution fonctionnelle des génomes ancestraux de vertébrés par duplication de gènes. Résumé Létude scientifique des processus biologiques sappuie généralement sur lobservation de résultats expérimentaux réalisés sur des modèles vivants. Cette évidence masque le fait que lensemble des organismes vivants est le fruit de centaines de millions dannées dévolution qui elles sont inaccessibles aux expériences, car situées dans le passé. Pour contribuer à rendre à la biologie cette dimension historique, notre laboratoire a entrepris de reconstruire une succession de génomes ancestraux, chez les vertébrés. Ici, nous exploitons à grande échelle les reconstitutions de génomes de vertébrés, afin de mettre en évidence, quantifier et dater les duplications de gènes au sein de différentes lignées de vertébrés. Ces duplications, en autorisant un relachement de la pression de sélection, ...
Looking for online definition of leukosis-sarcoma neoplastic diseases in the Medical Dictionary? leukosis-sarcoma neoplastic diseases explanation free. What is leukosis-sarcoma neoplastic diseases? Meaning of leukosis-sarcoma neoplastic diseases medical term. What does leukosis-sarcoma neoplastic diseases mean?
2Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892-4472 USA. Wnt1 signaling has been implicated as one factor involved in neural crest-derived melanocyte (NC-M) development. Mice deficient for both Wnt1 and Wnt3a have a marked deficiency in trunk neural crest derivatives including NC-Ms (1). We have used cell lineage-directed gene targeting of Wnt signaling genes to examine the effects of Wnt signaling in mouse neural crest development. Gene expression was directed to cell-lineages by infection with subgroup A avian leukosis virus vectors (RCAS) (2) in lines of transgenic mice that express the retrovirus receptor tv-a (2). Transgenic mice with tva in either nestin expressing neural precuror cells (line Ntva) or dopachrome tautomerase (DCT) expressing melanoblasts (line DCTtva) were analyzed. We over-stimulated Wnt signaling in two ways: directed gene transfer of Wnt1 to Ntva+ cells and transfer of b-catenin to DCTtva+ NC-M precursor ...
Cobalt increases the red cell mass in both man and animals by increasing the production of erythropoietin. Since meat-type chickens can develop pulmonary hypertension from increased erythropoiesis and polycythaemia, two trials were conducted to inves
Looking for Erythroblastosis faetalis? Find out information about Erythroblastosis faetalis. a disease manifested at birth or shortly thereafter and generally caused by incompatibility of the mothers blood with that of the fetus with respect to the... Explanation of Erythroblastosis faetalis
The diversity and polymorphisms in the ToMV population present in one ToMV-positive plant per genotype was further assessed. Total RNAs were isolated from the upper leaf of plants at 21 and 48 d.p.i. The complete ToMV ORF4 sequence was amplified using a two-step reverse-transcriptase polymerase chain reaction (RT-PCR) protocol. The first-strand cDNA was synthesized by reverse transcription of total RNAs using random hexamer primers and the Avian myeloblastosis virus reverse transcriptase (Promega Corp., Madison, WI, USA). A 480-bp fragment (corresponding to positions 5703-6182 of the complete ToMV reference sequence, NC_002692) was amplified using primer pair ToMV-F (5′-ATGTCTTACTCAATCACTTC-3′, sense) and ToMV-R (5′-TTAAGATGCAGGTGCAGAGG-3′, antisense) and the TaKaRa LA Taq polymerase (TaKaRa, Bio Inc., Shiga, Japan). PCR amplification was performed under the following cycling conditions: initial denaturation at 94°C for 5 min; 25 cycles of 94°C for 45 s, 54°C for 30 s, and 72°C for ...
Apffel, C A. and Peters, J H., "Successful heterotransplantation of l1210 mouse leukosis into un- treated adult hamsters. Abstr." (1968). Subject Strain Bibliography 1968. 45 ...
Looking for erythroblastoses? Find out information about erythroblastoses. The abnormal presence of erythroblasts in the blood. A virus disease of birds; considered to be part of the avian leukosis complex in which there is an... Explanation of erythroblastoses