1. The effect of a α-human atrial natriuretic peptide (1-28) (ANP) on human vasculature was investigated in vivo and in vitro. Possible involvement of vascular dopamine receptors and the renin-angiotensin system in the response to ANP was also studied in vivo.. 2. Forearm blood blow was measured by venous occlusion plethysmography. Isolated human blood vessels were studied using conventional organ bath techniques.. 3. ANP (0.1-1 μg/min, intra-arterially) produced a dose-dependent increase in forearm blood flow, corresponding to a 163% increase in net forearm blood flow in the study arm. This action of ANP was not antagonized by (R)-sulpiride (100 μg/min, intra-arterially), a selective vascular dopamine receptor antagonist, or 50 mg of oral captopril, an inhibitor of angiotensin-converting enzyme.. 4. ANP (1 nmol/l-1 μmol/l) produced concentration-dependent relaxation of isolated human arteries, including brachial artery, but was without effect on isolated human saphenous vein.. 5. ANP ...

TY - GEN. T1 - Acute hyperglycaemia causes elevation of plasma atrial natriuretic peptide concentrations in type 1 diabetes subjects. AU - McKenna, K. AU - Smith, D. AU - Tormey, William. AU - Thompson, CJ. PY - 1999. Y1 - 1999. M3 - Conference contribution. VL - 48. SP - A139-A139. BT - Unknown Host Publication. PB - John Wiley & Sons, Inc.. T2 - DIABETES. Y2 - 1 January 1999. ER - ...

The administration of exogenous atrial natriuretic peptide (ANP) resulted in numerous physiological responses, including a rapid natriuresis and diuresis as well as a reduction in arterial blood pressure. In order to provide a continuous supply of ANP, we used gene transfer approach to investigate the role of ANP in hypertensive rats and in normotensive animals with chronic heart failure. The human ANP gene placed under the control of RSV or CMV promoter with a 4F2 enhancer was incorporated into replication-deficient adenovirus. Expression of human ANP in cell culture or in rats was identified by RT-PCR Southern blot analysis and/or a radioimmunassay for human ANP. The results showed that a single injection of the ANP gene results in: 1) prolonged reduction of blood pressure in spontaneously hypertensive rats (SHR) and Dahl- salt sensitive (DSS) rats on a high salt diet, 2) increases in renal blood flow, glomerular filtration rate, sodium output, urine excretion and urinary cGMP levels, ...

alpha-Human atrial natriuretic peptide, a 28-amino-acid-residue peptide, was rapidly hydrolysed by pig kidney microvillar membranes in vitro, with a t1/2 of 8 min, comparable with the rate observed with angiotensins II and III. The products of hydrolysis were analysed by h.p.l.c., the pattern obtained with membranes being similar to that with purified endopeptidase-24.11 (EC 3.4.24.11). No hydrolysis by peptidyl dipeptidase A (angiotensin I converting enzyme, EC 3.4.15.1) was observed. The contribution of the various microvillar membrane peptidases was assessed by including specific inhibitors. Phosphoramidon, an inhibitor of endopeptidase-24.11, caused 80-100% suppression of the products. Captopril and amastatin (inhibitors of peptidyl dipeptidase A and aminopeptidases respectively) had no significant effect. Hydrolysis at an undefined site within the disulphide-linked ring occurred rapidly, followed by hydrolysis at other sites, including the Ser25--Phe26 bond. ...

Elevated plasma natriuretic peptide levels after AMI (acute myocardial infarction) are associated with adverse outcome. The aim of the present study was to examine the relationship of plasma N-ANP (N-terminal pro-atrial natriuretic peptide) and N-BNP (N-terminal pro-B-type natriuretic peptide) with mortality and heart failure following AMI. We studied 403 patients with AMI. Blood was sampled for measurement of N-ANP and N-BNP on a single occasion between 72 and 96 h after symptom onset. Natriuretic peptide levels were related to all-cause mortality and heart failure episodes. During follow up (median, 462 days; range 5-764), 43 (10.7%), 25 (6.2%) and 49 (12.2%) patients suffered death, heart failure hospitalization and outpatient heart failure respectively. Only N-BNP (P,0.0005), N-ANP (P=0.005) and previous AMI (P=0.016) were independently predictive of death. N-BNP, but not N-ANP, predicted 30-day mortality. N-ANP, but not N-BNP, predicted mortality after 30 days. N-BNP was the better ...

TY - JOUR. T1 - Atrial natriuretic polypeptide (ANP) in human ventricle increased gene expression of ANP in dilated cardiomyopathy. AU - Saito, Yoshihiko. AU - Nakao, Kazuwa. AU - Arai, Hiroshi. AU - Sugawara, Akira. AU - Morii, Narito. AU - Yamada, Takayuki. AU - Itoh, Hiroshi. AU - Shiono, Shozo. AU - Mukoyama, Masashi. AU - Obata, Kenji. AU - Yasue, Hirofumi. AU - Ohkubo, Hiroaki. AU - Nakanishi, Shigetada. AU - Imura, Hiroo. PY - 1987/10/14. Y1 - 1987/10/14. N2 - Tissue levels of atrial natriuretic polypeptide (ANP) messenger RNA (ANPmRNA) and ANP in the human atrium and ventricle were measured simultaneously by the blot hybridization technique and the specific radioimmunoassay for ANP. Hearts were obtained from two patients without cardiac complications and from a patient with dilated cardiomyopathy (DCM) at autopsy. Total RNA extracted from ventricles contained a hydridizing RNA band of the same size as atrial ANPmRNA in both control and DCM hearts. The ANPmRNA level in the control ...

Previous studies have shown that atrial natriuretic factor, a powerful vasorelaxant of precontracted vessels, inhibits the secretion of aldosterone stimulated by angiotensin II, adrenocorticotropic hormone, and potassium. We now report the presence of specific binding sites for atrial natriuretic factor in rat blood vessels (mesenteric and renal arteries) and adrenal capsules. Radioiodinated synthetic atrial natriuretic factor bound to a single class of high-affinity (KD = 0.1 nM) low-capacity receptors in a particulate fraction from blood vessels and adrenals. Unrelated peptides did not displace atrial natriuretic factor. Fragments of atrial natriuretic factor displaced the labeled ligand with decreasing potency after cleavage at the N-terminal. The cleavage of the C-terminal tyrosine did not decrease the potency of atrial natriuretic factor, but further cleavage at the C-terminal dramatically reduced the affinity of the resulting peptides. The potency of the atrial natriuretic factor fragments ...

1. The effects of the infusion of a low dose (2 pmol min−1 kg−1 for 3 h) of human atrial natriuretic peptide (hANP) were studied in seven healthy volunteers undergoing a water diuresis. Lithium clearance was used to monitor proximal tubular function.. 2. hANP increased urine flow rate, sodium, calcium and magnesium excretion without significant changes in potassium and phosphate excretion, heart rate or blood pressure.. 3. hANP caused a small change in fractional lithium clearance, and larger changes in distal nephron handling of sodium and water.. 4. Plasma renin activity tended to decrease during the infusion of hANP, while plasma aldosterone concentration decreased during and increased after stopping the infusion of hANP.. 5. The data suggest that hANP inhibits the reabsorption of sodium and water by an action on distal segments of the nephron and perhaps the proximal tubule. Inhibition of renin and aldosterone secretion may contribute to the natriuresis. ...

Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human N-Terminal Pro-Atrial Natriuretic Peptide (NT-ProANP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species ...

Plasma atrial natriuretic peptide in essential hypertension: effects of changes in dietary sodium. Sagnella, Giuseppe A.; Markandu, Nirmala D.; Buckley, Martin G.; Singer, Donald R.J.; Sugden, A. Louise; Shore, Angela C.; MacGregor, Graham A. // British Medical Journal (Clinical Research Edition);8/15/1987, Vol. 295 Issue 6595, p417 Examines the plasma atrial natriuretic peptide in essential hypertension. Effects of changes in dietary intake on plasma atrial peptides; Occurrence of change in plasma concentration of atrial peptide values during the transfer from normal to high sodium diet; Correlation between the fall in... ...

1. To study the anti-hypertensive effects of atrial natriuretic peptide (ANP), eight patients with mild to moderate essential hypertension, on no treatment, were infused with α-human ANP (102-126) (37 pmol min−1 kg−1) or placebo for 60 min and observed for a further 4 h on the fifth day of low and high sodium diets in a randomized, cross-over study.. 2. Plasma ANP levels increased over 30-fold into the high pathophysiological range during ANP infusion, but had returned to control values by 60 min after the end of infusion. With ANP infusion, there was a large decrease in supine blood pressure which was similar on both the low and high sodium intakes and was maximal 20-40 min after completion of the infusion. These reductions in blood pressure were sustained for a further 4 h after the end of ANP infusion and for 3 h after plasma ANP levels had returned to control values.. 3. Maximal urinary sodium excretion increased 10-fold on the low sodium diet (negative sodium balance 20 mmol) and ...

[Effects of female sex hormones on atrial natriuretic factor gene expression in rats].: To clarify the effects of female sex hormones on ANF gene expression, em

We investigated the hemodynamic effect of synthetic atrial natriuretic factor Auriculin A (ANF) and its influence on arterial baroreflex control of heart rate, systemic blood pressure, and perfusion pressure in the hind limb (perfused at constant flow) in rabbits anesthetized with alpha-chloralose and urethane. The neural mechanisms underlying these effects were also studied. In the intact animal, a 45-minute constant infusion of ANF (2 micrograms/kg prime, 0.2 microgram/kg/min) significantly reduced mean blood pressure and increased mean perfusion pressure, while heart rate did not change. Comparable data were obtained with lower (0.5 microgram/kg + 0.05 microgram/kg/min; 1 microgram/kg + 0.1 microgram/kg/min) or higher (4 micrograms/kg + 0.4 microgram/kg/min; 8 micrograms/kg + 0.8 microgram/kg/min) doses of ANF. In addition, ANF enhanced bradycardic reflex responses to phenylephrine i.v. bolus administration, while it did not change baroreflex-mediated responses to nitroglycerin i.v. bolus ...

Definition of atrial natriuretic factor in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is atrial natriuretic factor? Meaning of atrial natriuretic factor as a finance term. What does atrial natriuretic factor mean in finance?

To explore whether pathophysiological plasma levels of atrial natriuretic peptide (ANP) actually involve sodium excretion in spontaneously hypertensive rats (SHR), we examined the in vivo and ex vivo effects of ANP and an endopeptidase inhibitor, thiorphan, on urinary sodium excretion and the elimination rate of ANP. We found the following: 1) The basal plasma ANP level was higher in 16-week-old SHR than in Wistar-Kyoto (WKY) rats (109 +/- 10 [SEM] versus 63 +/- 4 pg/ml, p less than 0.001). Thiorphan (30 mg/kg i.v.) significantly increased plasma ANP by 60% in both SHR and WKY rats. However, increases in urinary sodium excretion (+290% versus +130%, p less than 0.05) and cyclic GMP (+160% versus +60%, p less than 0.05) were greater in SHR than in WKY rats. Urinary excretion of ANP was markedly increased by thiorphan, and its increase was greater in SHR than in WKY rats. 2) The thiorphan-induced natriuresis was substantially attenuated by antiserum for ANP but not by a bradykinin receptor ...

Renal ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury and a frequent occurrence in critically ill patients. Renal IRI releases proinflammatory cytokines within the kidney that induce crosstalk between the kidney and other organ systems. Atrial natriuretic peptide (ANP) has anti-inflammatory as well as natriuretic effects and serves important functions as a regulator of blood pressure, fluid homeostasis, and inflammation. The objective of the present study was to elucidate whether ANP post-treatment attenuates kidney-lung-heart crosstalk in a rat model of renal IRI. In experiment I, a rat model of unilateral renal IRI with mechanical ventilation was prepared by clamping the left renal pedicle for 30 min. Five minutes after clamping, saline or ANP (0.2 μg/kg/min) was infused. The hemodynamics, arterial blood gases, and plasma concentrations of lactate and potassium were measured at baseline and at 1, 2, and 3 h after declamping. The mRNA expression and localization of tumor

METHODS AND RESULTS Twenty patients with acute right ventricular infarction were examined who underwent reperfusion therapy on admission. These patients had proximal occlusion of the dominant right coronary artery involving the right atrial branches: 9 patients with successful reperfusion (SRP group) and the remaining 11 patients with unsuccessful reperfusion (URP group). Within 24 hours after the onset of infarction, a volume loading test was performed after reperfusion therapy with measurements for plasma ANP levels and hemodynamics. Before the volume loading test, the plasma ANP level and mean right atrial pressure were similar between these two groups. However, in the URP group, percent increase in ANP in response to volume loading was strikingly smaller (URP, 45 +/- 18% versus SRP, 133 +/- 25%; P , .01) despite similar percent increase in mean right atrial pressure (URP, 100 +/- 46% versus SRP, 86 +/- 23%). The peak ANP level occurred significantly later in the URP group (69 +/- 16 hours) ...

In response to a high salt intake, salt-sensitive hypertensive individuals retain more sodium and manifest a rise in blood pressure greater than that in salt-resistant individuals. In this study, we tested whether salt sensitivity might be related at least in part to reduced secretion of atrial natriuretic peptide (ANP) or to abnormal nitric oxide production. We measured plasma ANP and NO2+NO3 in 7 normotensive individuals and 13 salt- sensitive and 14 salt-resistant blacks with essential hypertension under conditions of low (10 mEq/d) and high (250 mEq/d) salt intake. To evaluate possible racial differences in ANP secretion, we also measured plasma ANP in 6 salt-sensitive and 8 salt-resistant hypertensive whites during low and high salt intakes. Under low salt conditions, plasma ANP levels were not different in normotensive control subjects and salt-sensitive and salt-resistant hypertensive blacks. During high salt intake, plasma ANP levels did not change in control subjects and salt-resistant ...

A 75-year-old Japanese man was hospitalized for hypokalemia. Levels of plasma adrenocorticotropic hormone (ACTH) and free cortisol in urine were elevated to 220 pg/mL (normal, 9 to 52 pg/mL) and 793 mg/d (normal, 30 to 100 mg/d), respectively. Blood concentration of corticotropin-releasing factor was 13.8 pg/mL (normal, ,40 pg/mL). Chest radiographs and computed tomographic examinations showed an enlarged thymic gland and normal-sized adrenal glands. Venous samplings in the superior and inferior vena cava at several levels showed markedly increased plasma atrial natriuretic peptide concentrations near the thymic tumor (from 120 to 620 pg/mL). By contrast, plasma ACTH concentrations were constant at all sites sampled. Pathologic study results were compatible with carcinoid. Immunohistochemical staining for atrial natriuretic peptides and ACTH in the dissected tumor tissue showed several cells to be positively stained for the peptides. No ACTH-containing cells were detected. After the tumor was ...

TY - JOUR. T1 - Salt intake and plasma atrial natriuretic peptide and nitric oxide in hypertension. AU - Campese, Vito M.. AU - Tawadrous, Medhat. AU - Bigazzi, Roberto. AU - Bianchi, Stefano. AU - Mann, Amardeep S.. AU - Oparil, Suzanne. AU - Raij, Leopoldo. PY - 1996/9. Y1 - 1996/9. N2 - In response to a high salt intake, salt-sensitive hypertensive individuals retain more sodium and manifest a rise in blood pressure greater than that in salt-resistant individuals. In this study, we tested whether salt sensitivity might be related at least in part to reduced secretion of atrial natriuretic peptide (ANP) or to abnormal nitric oxide production. We measured plasma ANP and NO2+NO3 in 7 normotensive individuals and 13 salt- sensitive and 14 salt-resistant blacks with essential hypertension under conditions of low (10 mEq/d) and high (250 mEq/d) salt intake. To evaluate possible racial differences in ANP secretion, we also measured plasma ANP in 6 salt-sensitive and 8 salt-resistant hypertensive ...

TY - JOUR. T1 - Exertional Changes in Circulating Cardiac Natriuretic Peptides in Patients with Suggested Coronary Artery Disease. AU - Bergeron, Sébastien. AU - Møller, Jacob E.. AU - Bailey, Kent R.. AU - Chen, Horng H.. AU - Burnett, John C.. AU - Pellikka, Patricia A.. PY - 2006/6/1. Y1 - 2006/6/1. N2 - Background: We measured plasma brain natriuretic peptide (BNP) and N-terminal atrial natriuretic peptide (ANP) levels before and after exercise stress testing and correlated results with echocardiographic evidence of ischemia. Methods: Sixty patients with left ventricular ejection fraction greater than 50% referred for clinically indicated exercise echocardiogram were studied. Peptides were measured at rest and 5 minutes after symptom-limited exercise. Results: Echocardiography was positive for ischemia in 19 (32%). With exercise, ANP level increased in all 60 patients (median at rest 2501 [799-6440]-3167 [977-8563] pg/mL after exercise [P , .0001]). BNP increased in 54 patients (90%) (19 ...

TY - JOUR. T1 - Atrial natriuretic peptide in hypothyroidism. AU - Zimmerman, R. S.. AU - Gharib, H.. AU - Zimmerman, D.. AU - Heublein, D.. AU - Burnett, J. C.. PY - 1987/2. Y1 - 1987/2. N2 - The effect of hypothyroidism on circulating levels of atrial natriuretic peptide (ANP) was studied in 11 hypothyroid patients (aged 11-65 yr; mean, 31 yr) and 13 normal subjects (aged 28-39 yr; mean, 32 yr). Plasma ANP was 32 ± 9 (±sd) pg/ml in normal subjects and 20 ± 5 pg/ml in the hypothyroid patients (P , 0.005). In 7 hypothyroid patients, plasma ANP levels were measured after 10-14 weeks of l-T4 therapy. ANP increased from 22 ± 5 to 46 ± 18 pg/ml (P , 0.02), along with an increase in mean serum T4 from 0.6 ± 0.5 to 8.1 ± 2.5 μg/dl (P , 0.001). Thus, hypothyroidism is characterized by decreased circulating levels of ANP which are reversed by l-T4 therapy.. AB - The effect of hypothyroidism on circulating levels of atrial natriuretic peptide (ANP) was studied in 11 hypothyroid patients (aged ...

The aim of the present study was to determine whether left atrial size--a likely indicator of atrial stretching--correlates with the plasma concentration of atrial natriuretic peptide and whether this relation is different in patients in sinus rhythm and in those with atrial fibrillation. Arterial plasma concentrations of immunoreactive atrial natriuretic peptide (ir-ANP), adrenaline, noradrenaline, aldosterone, and vasopressin were measured in 13 patients in sinus rhythm without apparent heart failure and in 13 patients in atrial fibrillation. The two groups were matched for left atrial diameter and the ratio of the left atrial diameter to the diameter of the aortic root (assessed by echocardiography). There were no significant differences in age, heart rate, blood pressure, or left ventricular end diastolic diameter between the two groups. Left atrial diameters varied from 33 to 60 mm. The mean (SD) plasma concentration of ir-ANP was significantly higher (35 (21) pmol/l) in the patients with ...

SWISS-MODEL Template Library (SMTL) entry for 7brj.1. Atrial Natriuretic Peptide Receptor complexed with deletion mutant of human Atrial Natriuretic Peptide[7-28]

The influence of physiological cardiac hypertrophy on the concentration of plasma atrial natriuretic peptide was studied in six male athletes and six normally active, matched control men. They were examined by echocardiography during a graded exercise test on a bicycle ergometer. Plasma atrial natriuretic peptide was measured at rest, at each workload until exhaustion, and 15 and 30 minutes after the exercise test. Echocardiography showed that the athletes had a significantly larger left atrium, left ventricular end diastolic diameter, left ventricular posterior wall, interventricular septum, left ventricular ejection fraction, and left ventricular mass than the controls. The athletes performed significantly more work than the control group--325 W v 277 W. The plasma concentration of atrial natriuretic peptide rose by a mean factor of 2.76 (range 1.78-4.28) in all men from rest to maximum exercise. There were no differences between the athletes and the controls in the concentrations of plasma ...

Synonyms for Atrial natriuretic peptide in Free Thesaurus. Antonyms for Atrial natriuretic peptide. 4 words related to peptide: amide, fibrinopeptide, polypeptide, endorphin. What are synonyms for Atrial natriuretic peptide?

The effects of acute volume expansion and acute salt loading on the plasma levels of immunoreactive atrial natriuretic peptides (ir-ANPs) were investigated in conscious adult (16 weeks) Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR). Basal plasma concentrations of ir-ANPs were similar (108 +/- 9 pg/ml and 105 +/- 8 pg/ml, respectively) in hypertensive and normotensive rats. In both strains mean arterial pressure, heart rate and plasma catecholamines were unaltered by acute volume expansion but significantly elevated by acute salt loading. However, both acute volume expansion and acute salt loading increased plasma concentrations of ir-ANPs similarily in SHR and WKY rats, independent of blood pressure or plasma catecholamine levels. Our findings show that chronic hypertension in SHR does not result in changes in basal or VOL- and SAL-stimulated levels of circulating ir-ANPs.

PubMed Central Canada (PMC Canada) provides free access to a stable and permanent online digital archive of full-text, peer-reviewed health and life sciences research publications. It builds on PubMed Central (PMC), the U.S. National Institutes of Health (NIH) free digital archive of biomedical and life sciences journal literature and is a member of the broader PMC International (PMCI) network of e-repositories.

திறன்மிகு நாள விரிப்பியான (Vasodilator) இதயியச் சோடியச்சிறுநீர்மைப் புரதக்கூறு [Atrial natriuretic peptide; (ANP), atrial natriuretic factor; (ANF), atrial natriuretic hormone; (ANH), Cardionatrine, Cardiodilatine; (CDD), atriopeptin], இதயத்தசைச் செல்களால் சுரக்கப்படும் புரதக்கூற்று இயக்குநீராகும்[2][3][4]. இப்புரதக்கூறானது உடலின் நீர், சோடியம், பொட்டாசியம், கொழுப்புத் திசுக்களில் உள்ள கொழுப்பு ஆகியவற்றின் சமநிலையைக் கட்டுபடுத்துவதில் ஈடுபட்டுள்ளது; இரத்தத்தின் கன அளவு ...

The clinical significance of cardiac Natriuretic Peptides (cNP) plasma levels has been shown to differ in obese and non-obese subjects. Recent lines of evidence suggest important metabolic effects of the cNP system, which has been shown to activate lipolysis, enhance lipid oxidation and mitochondrial respiration. Clinically, these properties lead to browning of white adipose tissue and to increased muscular oxidative capacity. In patients without heart disease higher cNP concentrations were observed in lean, insulin-sensitive subjects, while obese and type-2 diabetics may display reduced cNP levels.

Occurrence of a novel cardiac natriuretic peptide in rats.: We established a specific radioimmunoassay for the ring structure of iso-ANP and detected iso-ANP[

TY - JOUR. T1 - Atrial natriuretic factor modulates whole kidney tubuloglomerular feedback. AU - Margulies, K. B.. AU - Burnett, John C Jr.. PY - 1990. Y1 - 1990. N2 - Increases in sodium delivery to the distal nephron reduce glomerular filtration rate (GFR) via tubuloglomerular feedback (TGF). Central volume expansion and pharmacological concentrations of atrial natriuretic factor (ANF) are known to attenuate this response. To test the hypothesis that whole kidney TGF is attenuated by pathophysiological concentrations of ANF, hypertonic saline was given intrarenally in five dogs receiving an intravenous infusion of synthetic ANF at 20 ng·kg-1·min-1. To examine whole kidney TGF responses in heart failure, six additional dogs with acute congestive heart failure induced by rapid ventricular pacing also received intrarenal hypertonic saline. Seven sham-paced dogs served as controls. An isolated increase in circulating ANF from 26 ± 3 to 342 ± 23 pg/ml abolished the whole kidney GFR response to ...

BACKGROUND: Natriuretic peptides are currently used in the diagnosis and follow-up of patients with Chronic Heart Failure (CHF). However, it is unknown whether there are different influences of age on atrial natriuretic peptide (ANP)/N-terminal-ANP (NT-ANP) or B-type natriuretic peptide (BNP)/N-terminal-proBNP (NT-proBNP). AIMS: To compare the influence of age and gender on plasma levels of ANP/NT-ANP and BNP/NT-proBNP in CHF patients. METHODS AND RESULTS: Natriuretic peptides were measured in 311 CHF patients (68+/-8 years, 76% males, left ventricular ejection fraction (LVEF) 0.23+/-0.08). All natriuretic peptides were significantly related to age (p,0.05) on multivariate regression analysis, with partial correlation coefficients of 0.18, 0.29, 0.28 and 0.25 for ANP, NT-ANP, BNP and NT-proBNP, respectively. The relative increase of both BNP/NT-proBNP were more pronounced than of ANP/NT-ANP (p,0.01). Furthermore, the relative increase of BNP with age was markedly larger than of NT-proBNP ...

The role of atrial natriuretic factor (ANF) in modulating volume and circulatory homeostasis remains uncertain, and there has been as yet no systematic analysis of the factors promoting ANF release in humans. Since immersion in water to the neck provides a volume stimulus identical to that induced by 2 litres of saline, without plasma compositional change, immersion to the neck was used to assess the ANF response to acute central blood-volume expansion. Using a radio-immunoassay that reliably detected ANF in human plasma extracts, more than 80% of plasma immunoreactive (ir) ANF was shown to elute as a single peak on reverse-phase high performance liquid chromatography, with a retention time identical to that of the synthetic 28-residue alpha-human (alpha-h) ANF. The response of plasma irANF to 3 h of immersion in water to the neck was evaluated in four sodium-replete normal subjects; the immersion produced a prompt and marked increase in irANF in each subject, and recovery was associated with a prompt

BACKGROUND--Plasma levels of atrial natriuretic peptide (ANP) are elevated in patients with chronic obstructive pulmonary disease (COPD) and may have a role in preventing oedema formation in these patients. METHODS--Plasma ANP levels were measured in 60 patients with COPD and these measurements were related to pulmonary haemodynamics, response to treatment during exacerbations, and clinical patterns of the stable disease. RESULTS--Plasma ANP levels did not correlate significantly with right atrial or pulmonary arterial pressures but did correlate significantly with both the right ventricular end diastolic volume and right ventricular wall volume measured by magnetic resonance imaging. Oxygen (2 1/min by nasal prongs for 30 minutes) did not change the mean pulmonary arterial pressure or the level of plasma ANP. In 20 patients with an acute exacerbation of COPD plasma ANP levels were higher in those with oedema (302 (185) pg/ml) than in those without oedema (87 (43) pg/ml). Oxygen given for one ...

Title:The Emerging Role of Atrial Natriuretic Peptide in Psychiatry. VOLUME: 28 ISSUE: 1. Author(s):Donatella Marazziti, Filippo Maria Barberi, Federico Mucci*, Alessandra Maglio, Valerio DellOste and Liliana DellOsso. Affiliation:Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa, Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa, Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa, Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa, Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa, Dipartimento di Medicina Clinica e Sperimentale, Section of Psychiatry, University of Pisa, Pisa. Keywords:Atrial natriuretic peptide, depression, anxiety disorder, post-traumatic stress disorder, schizophrenia, alcohol abuse, ANP.. Abstract:Introduction: Atrial natriuretic peptide ...

TY - JOUR. T1 - Physiological features of edematous dogs unresponsive to atrial natriuretic peptide. AU - Maher, Elizabeth. AU - Cernacek, Peter. AU - Levy, Mortimer. PY - 1990. Y1 - 1990. N2 - Sodium-retaining cirrhotic and chronic caval dogs with ascites show a heterogeneous natriuretic response to atrial natriuretic factor (ANF) infusions such that half will increase their urinary excretion of sodium and half will show no natriuretic response whatsoever. In these studies we have examined several physiological variables that might discriminate between these two experimental populations. We studied 22 caval dogs (11 natriuretic responders, 11 nonresponders) and 19 cirrhotic dogs (9 responders, 10 nonresponders). After an infusion of rat ANP-(1-28), 125 ng·kg-1·min-1, differences in glomerular filtration rate, blood pressure, or urinary excretion of guanosine 3,5-cyclic monophosphate (cGMP) could not differentiate between the two types of dogs. When the left kidney of nonresponding dogs in ...

The physiological actions of the atrial peptide system are discussed in relation to its potential role in the aetiology and treatment of hypertension and congestive heart failure (CHF). Atrial natriuretic factor (ANF) exerts marked natriuretic, diure

To evaluate the role of calcium in volume-induced secretion of atrial natriuretic factor (ANF), effects of verapamil and endothelin-1 (ET-1), both known to influence free intracellular calcium, were studied during saline infusion in seven conscious instrumented dogs. Fifteen minutes after intravenous injection of placebo or verapamil (0.25 mg/kg) or during continuous ET-1 infusion (at 5 ng.kg-1.min-1), saline (10% body wt) was infused during 40 min. Saline produced a rise (P less than 0.001) of plasma ANF from 28.1 +/- 6.3 to 50.4 +/- 12.9 pM after placebo, from 30.2 +/- 6.1 to 51.1 +/- 8.5 pM after verapamil, and from 31.2 +/- 6.1 to 81.0 +/- 12.9 pM with ET-1. This increase was comparable after placebo and verapamil, but was 80% greater with ET-1 (P less than 0.02). Plasma ET-1, unchanged after placebo, rose (P less than 0.001) from 1.7 +/- 0.5 to 38.3 +/- 9.2 pM with ET-1. In the three experiments, heart rate and left atrial pressure (LAP) increased (P less than 0.001) similarly. The linear ...

Learn in-depth information on Atrial Natriuretic Peptide Blood Test, on why the laboratory test is performed, specimen collected, the significance of the results, and its use in diagnosing medical conditions.

N2 - *Atrial natriuretic factor (ANP) has shown over recent decades to play an emerging role in the regulation of coronary blood flow as well as systemic blood pressure. The common polymorphism in the atrial natriuretic peptide precursor gene (NPPA), +2238 T/C, was in the previous studies associated with hypertension as well as other cardiovascular conditions. The purpose of the study was to identify the possible relationship between the +2238 T/C polymorphism and restenosis in coronary arteries stented due to the coronary artery disease (CAD ...

Top Domain Names wwwnatriuretic peptide.com ww.natriuretic peptide.com 333.natriuretic peptide.com 2ww.natriuretic peptide.com qww.natriuretic peptide.com wqwnatriuretic peptide.com wwa.natriuretic peptide.com mail.natriuretic peptide.com pop.natriuretic peptide.com vebmail.natriuretic peptide.com http:natriuretic peptide.com htp://natriuretic peptide.com http::/natriuretic peptide.com rttp://natriuretic peptide.com hftp://natriuretic peptide.com htgp://natriuretic peptide.com htt[://natriuretic peptide.com https:/natriuretic peptide.com https/.natriuretic peptide.com htttp/natriuretic peptide.com vvv.natriuretic peptide.com membersnatriuretic peptide.com www.webmail.natriuretic peptide.com www .natriuretic peptide.com htp://www.natriuretic peptide.com http::/www.natriuretic peptide.com rttp://www.natriuretic peptide.com hftp://www.natriuretic peptide.com htgp://www.natriuretic peptide.com htt[://www.natriuretic peptide.com https:/www.natriuretic peptide.com https//.www.natriuretic peptide.com ...

BACKGROUND: The natriuretic peptides are counterregulatory hormones involved in volume homeostasis and cardiovascular remodeling. The prognostic significance of plasma natriuretic peptide levels in apparently asymptomatic persons has not been established.. METHODS: We prospectively studied 3346 persons without heart failure. Using proportional-hazards regression, we examined the relations of plasma B-type natriuretic peptide and N-terminal pro-atrial natriuretic peptide to the risk of death from any cause, a first major cardiovascular event, heart failure, atrial fibrillation, stroke or transient ischemic attack, and coronary heart disease.. RESULTS: During a mean follow-up of 5.2 years, 119 participants died and 79 had a first cardiovascular event. After adjustment for cardiovascular risk factors, each increment of 1 SD in log B-type natriuretic peptide levels was associated with a 27 percent increase in the risk of death (P=0.009), a 28 percent increase in the risk of a first cardiovascular ...

TY - JOUR. T1 - Comparative effects of atrial natriuretic peptide and brain natriuretic peptide on the aldosterone and pressor responses to angiotensin II in man. AU - Cargill, Robert I.. AU - Struthers, Allan D.. AU - Lipworth, Brian J.. PY - 1995. Y1 - 1995. N2 - 1. Atrial natriuretic peptide and brain natriuretic peptide have similar vasodilator and natriuretic properties, although little information is available regarding their relative effects as antagonists of the renin-angiotensin-aldosterone system. We have therefore compared how atrial natriuretic peptide and brain natriuretic peptide affect the systemic pressor and aldosterone responses to angiotensin II in eight male subjects. 2. Each subject was studied on three separate occasions, when they received a 60-min infusion of placebo, atrial natriuretic peptide (10 pmol min-1 kg-1) or brain natriuretic peptide (10 pmol min-1 kg-1), with a concomitant infusion of angiotensin II (6 ng min-1 kg-1) given for the final 30 min of the infusion ...

TY - JOUR. T1 - Atrial natriuretic factor inhibits Na-K-Cl cotransport in teleost intestine. AU - OGrady, S. M.. AU - Field, M.. AU - Nash, N. T.. AU - Rao, M. C.. PY - 1985/1/1. Y1 - 1985/1/1. N2 - Addition of atrial natriuretic factor (ANF) to the contraluminal side of the intestinal mucosa of a marine teleost, the winter flounder Pseudopleuronectes americanus, inhibits short-circuit current, net transepithelial fluxes of Na and Cl, and the unidirectional influx of Rb across the brush border membrane. This action of ANF is closely mimicked by addition of 8-bromo-guanosine 3,5-cyclic monophosphate (8-BrcGMP). In contrast to the intestine, the opercular epithelium of the flounder did not respond to the in vitro addition of either ANF or 8-BrcGMP. Because intestinal salt and water absorption diminishes when marine fish enter water of lower salinity, ANF may be an important hormonal regulator through which euryhaline fish adapt to varying salinities.. AB - Addition of atrial natriuretic factor ...

Natriuretic peptide precursor C, also known as NPPC, is a protein that in humans is encoded by the NPPC gene. The precursor NPPC protein is cleaved to the 22 amino acid peptide C-type natriuretic peptide (CNP). Natriuretic peptides comprise a family of 3 structurally related molecules: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP), encoded by a gene symbolized NPPC. These peptides possess potent natriuretic, diuretic, and vasodilating activities and are implicated in body fluid homeostasis and blood pressure control. Unlike ANP and BNP, CNP does not have direct natriuretic activity. This is because CNP is a selective agonist for the B-type natriuretic receptor (NPRB) whereas ANP and BNP are selective for the A-type natriuretic receptor (NPRA). GRCh38: Ensembl release 89: ENSG00000163273 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000026241 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: ...

TY - JOUR. T1 - Hemodynamic and renal excretory effects of human brain natriuretic peptide infusion in patients with congestive heart failure. T2 - A double-blind, placebo-controlled, randomized crossover trial. AU - Marcus, Lee S.. AU - Hart, Douglas. AU - Packer, Milton. AU - Yushak, Madeline. AU - Medina, Norma. AU - Danziger, Robert S.. AU - Heitjan, Daniel F.. AU - Katz, Stuart D.. PY - 1996. Y1 - 1996. N2 - Background: The pharmacological effects of infusion of human brain natriuretic peptide (hBNP) in patients with severe congestive heart failure have not been characterized previously. Methods and Results: Twenty patients with severe congestive heart failure were randomized in a double-blind, placebo-controlled, crossover trial to receive incremental 90-minute infusions of hBMP (0.003, 0.01, 0.03, and 0.1 μg/kg per minute) or placebo on 2 consecutive days. At the highest completed dose of the hBNP, mean pulmonary artery pressure decreased from 38.3±1.6 to 25.9±1.7 mm Hg; mean pulmonary ...

Frossard et al. (1997) studied an insertion/deletion (I/D) dimorphism located in the second intron of the human atrial natriuretic factor (ANF) gene among 232 UAE nationals (112 normotensives and 120 hypertensives) from the Abu Dhabi Emirate, with a view to evaluating the value of this marker in relation to hypertension. Results reveal that genotype frequencies of this I/D marker occur in Hardy-Weinberg proportions (respective genotype frequencies in the overall sample population are: II, 51%; ID, 42%; DD, 7%). No association, however, was evidenced between this dimorphic site and clinical diagnosis of essential hypertension. This suggests that: 1) this I/D dimorphism is not a useful marker to study the relationship between the ANF gene and hypertension in the UAE; and 2) variations of the ANF gene that may be in linkage disequilibrium with this marker do not play a major role in the determination of hypertension in this Arab population. Obineche et al. (2002) carried out a case-controlled study ...

TY - JOUR. T1 - C-type natriuretic peptide/guanylate cyclase B system in ATDC5 cells, a chondrogenic cell line. AU - Suda, Michio. AU - Tanaka, Kiyoshi. AU - Yasoda, Akihiro. AU - Komatsu, Yasato. AU - Chusho, Hideki. AU - Miura, Masako. AU - Tamura, Naohisa. AU - Ogawa, Yoshihiro. AU - Nakao, Kazuwa. PY - 2002/12/1. Y1 - 2002/12/1. N2 - Natriuretic peptides constitute a family of three structurally related peptides: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP), and C-type natriuretic peptide (CNP). Particulate guanylate cyclases, GC-A, and GC-B, are the receptors for these peptides to mediate their action. ANP and BNP possess high affinities for GC-A, and CNP is the preferred ligand for GC-B. In this article, we report our study of the expression and possible role(s) of natriuretic peptides in ATDC5 cells, which represent a chondrogenic cell line. ATDC5 cells produced cyclic guanosine monophosphate (cGMP) in response to natriuretic peptides. CNP was far more potent than ANP ...

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP. May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects. May regulate diuresis, blood pressure and skeletal development. Does not have guanylate cyclase activity.

The measurement of natriuretic peptides for the diagnosis of heart failure has been a major breakthrough in cardiology (1)(2). B-Type natriuretic peptide (BNP) is synthesized as preproBNP mainly in the ventricular myocardium. On ventricular myocyte stretch, preproBNP is enzymatically cleaved to proBNP and released in the form of the hormonally active BNP and the inactive N-terminal proBNP (NT-proBNP). Both BNP and NT-proBNP have been shown to reflect heart failure severity (1), but studies on their sensitivity and specificity for different degrees of heart failure produced conflicting results (3)(4)(5)(6). Both BNP and NT-proBNP can be used for the diagnosis of heart failure, but there are important differences between the two tests, particularly regarding influence of age and renal function (1). In addition to glomerular filtration, BNP is eliminated from plasma mainly through natriuretic peptide receptors and degraded by neutral endopeptidases (7)(8)(9). In contrast, NT-proBNP possibly is ...

TY - JOUR. T1 - Rat brain natriuretic peptide. T2 - Isolation from rat heart and tissue distribution. AU - Nakao, Kazuwa. AU - Itoh, Hiroshi. AU - Kambayashi, Yoshikazu. AU - Hosoda, Kiminori. AU - Saito, Yoshihiko. AU - Yamada, Takayuki. AU - Mukoyama, Masashi. AU - Arai, Hiroshi. AU - Shirakami, Gotaro. AU - Suga, Shin Ichi. AU - Jougasaki, Michihisa. AU - Ogawa, Yoshihiro. AU - Inouye, Ken. AU - Imura, Hiroo. PY - 1990/6. Y1 - 1990/6. N2 - We have isolated a cardiac natriuretic peptide of 5, 000 d from atrial tissues from 500 rats and determined its amino acid sequence. The 5, 000 d atrial natriuretic factor was elucidated to be a 45 amino acid peptide with the sequence of S-Q-D-S-A-F-R-I-Q-K-R-L-R-N-S-K-M-A-H-S-S-S-C-F-G-Q-K-I-D-R-I-G-A-V-S-R-L-G-C-D-G-L-R-L-F by sequencing the native peptide and its lysyl endopeptidase digests. The sequence of this peptide was identical to the amino acid sequence (51-95) of the rat brain natriuretic peptide precursor deduced from the complementary DNA ...

Brain natriuretic peptide (BNP) was originally identified in extracts of porcine brain. However, in humans, it is produced mainly in the cardiac ventricles in response to excessive stretching of heart muscle cells (cardiomyocytes); high concentrations of BNP in the blood are a marker of heart failure. BNP is also known as natriuretic peptide B, gamma-brain natriuretic peptide, and GC-B. The release of BNP is controlled by calcium ions. BNP binds to and activates the atrial natriuretic factor receptors NPRA and NPRB, similar to atrial natriuretic peptide (ANP) but with much lower affinity. Both BNP and ANP cause a net decrease in blood volume, which lowers the bodys blood pressure. Recombinant BNP is used to treat decompensated heart failure.. ...

Brain natriuretic peptide (BNP) was originally identified in extracts of porcine brain. However, in humans, it is produced mainly in the cardiac ventricles in response to excessive stretching of heart muscle cells (cardiomyocytes); high concentrations of BNP in the blood are a marker of heart failure. BNP is also known as natriuretic peptide B, gamma-brain natriuretic peptide, and GC-B. The release of BNP is controlled by calcium ions. BNP binds to and activates the atrial natriuretic factor receptors NPRA and NPRB, similar to atrial natriuretic peptide (ANP) but with much lower affinity. Both BNP and ANP cause a net decrease in blood volume, which lowers the bodys blood pressure. Recombinant BNP is used to treat decompensated heart failure.. ...

BACKGROUND: In mammalian follicles, oocytes are arrested at the diplotene stage of prophase I until meiotic resumption following the LH surge. Recently, C-type natriuretic peptide (CNP), encoded by natriuretic peptide precursor type C (NPPC) was found to suppress mouse oocyte maturation by promoting cyclic guanosine 5-monophospate (cGMP) production in cumulus cells. However, regulation of NPPC/CNP expression during the pre-ovulatory period and their regulation by the LH surge have not been investigated.. METHODS AND RESULTS: Based on genome-wide analysis of DNA microarray data sets using samples from periovulatory ovaries, we found increases in NPPC transcripts in granulosa cells during pre-ovulatory follicle growth in mice and a rapid decline induced by the pre-ovulatory LH/hCG stimulation. Treatment of pre-ovulatory animals with hCG decreased ovarian CNP content. In isolated ovarian cells, NPPC mRNA was predominantly expressed in mural granulosa cells exhibiting similar regulation following ...

Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.

Brain natriuretic peptide (BNP), also known as B-type natriuretic peptide, is a hormone secreted by cardiomyocytes in the heart ventricles in response to stretching caused by increased ventricular blood volume. BNP is named as such because it was originally identified in extracts of pig brain. The 32-amino acid polypeptide BNP is secreted attached to a 76-amino acid N-terminal fragment in the prohormone called NT-proBNP (BNPT), which is biologically inactive. Once released, BNP binds to and activates the atrial natriuretic factor receptor NPRA, and to a lesser extent NPRB, in a fashion similar to atrial natriuretic peptide (ANP) but with 10-fold lower affinity. The biological half-life of BNP, however, is twice as long as that of ANP, and that of NT-proBNP is even longer, making these peptides better targets than ANP for diagnostic blood testing. The physiologic actions of BNP are similar to those of ANP and include decrease in systemic vascular resistance and central venous pressure as well as ...

TY - JOUR. T1 - Atrial natriuretic peptide inhibits angiotensin II-stimulated proliferation in fetal cardiomyocytes. AU - OTierney, P. F.. AU - Chattergoon, N. N.. AU - Louey, S.. AU - Giraud, G. D.. AU - Thornburg, K. L.. N1 - Copyright: Copyright 2012 Elsevier B.V., All rights reserved.. PY - 2010/8. Y1 - 2010/8. N2 - The role of atrial natriuretic peptide (ANP) in regulating fetal cardiac growth is poorly understood. Angiotensin II (Ang II) stimulates proliferation in fetal sheep cardiomyocytes when growth is dependent on the activity of the mitogen-activated protein kinase (MAPK) and phosphoinositol-3-kinase (PI3K) pathways. We hypothesized that ANP would suppress near-term fetal cardiomyocyte proliferation in vitro and inhibit both the MAPK and PI3K pathways. Forty-eight hour 5-bromodeoxyuridine (BrdU) uptake (used as an index of proliferation) was measured in cardiomyocytes isolated from fetal sheep (135 day gestational age) in response to 100 nmAng IIwith orwithoutANP(0.003-100 nm) or 1 ...

Results: ET-1 levels were significantly higher in primary hypertension (9.93 ± 1.73 pg/ml) and renal hypertension (10.77 ± 1.50 pg/ml) in comparison to controls (4.03 ± 0.97 pg/ml), (p , 0.001, p , 0.001, respectively). NT-pro ANP concentrations in primary hypertension (71.03 ± 10.02 pg/ml), and renal hypertension (84.78 ± 6.44 pg/ml) were significantly higher than in the control group (29.62 ± 5.56 pg/ml) (p , 0.001, p , 0.001, respectively). TNF-α concentrations in primary hypertension (8.36 ± 1.60 pg/ml) and renal hypertension (7.35 ± 0.93 pg/ml) significantly exceeded concentrations in controls (4.49 ± 0.93 pg/ml), (p , 0.001, p , 0.001, respectively). ET-1 and NT-pro ANP concentrations in renal hypertension significantly exceeded those in primary hypertension (p = 0.049, p , 0.001, respectively) while TNF-α levels in renal hypertension were significantly lower than in primary hypertension (p = 0.046 ...

Natriuretic Peptides: Peptides that regulate the WATER-ELECTROLYTE BALANCE in the body, also known as natriuretic peptide hormones. Several have been sequenced (ATRIAL NATRIURETIC FACTOR; BRAIN NATRIURETIC PEPTIDE; C-TYPE NATRIURETIC PEPTIDE).

Hypoxic-ischaemic brain injury at birth is associated with 1-3/1000 cases of moderate to severe encephalopathy. Previously, we have shown that connexin 43 hemichannel blockade, with a specific mimetic peptide, reduced the occurrence of seizures, improved recovery of EEG power and sleep state cycling, and improved cell survival following global cerebral ischaemia. In the present study, we examined the dose response for intracerebroventricular mimetic peptide infusion (50 µmol/kg/h for 1 h, followed by 50 µmol/kg/24 h (low dose) or 50 µmol/kg/h for 25 h (high dose) or vehicle only (control group), starting 90 min after the end of ischaemia), following global cerebral ischaemia, induced by 30 min bilateral carotid artery occlusion, in near-term fetal sheep (128 ± 1 days gestation). Both peptide infusion groups were associated with a transient significant increase in EEG power between 2-12 h after ischaemia. The ischaemia-low dose group showed a significant recovery of EEG power from day five compared

Previous studies have shown that natriuretic peptide levels correlate inversely with GFR in chronic kidney disease (16,17). Investigators have postulated that higher filling pressures and wall stress play an important role in this cardiorenal link. However, in our patients with renal failure (75% acute), natriuretic peptide levels were massively elevated and could not be explained on the basis of higher filling pressures, wall stress, or depressed cardiac function. Moreover, natriuretic peptide levels ,1,000 pg/ml (BNP) and 10,000 pg/ml (NT-proBNP) were more specific for a GFR ,60 (BNP, 92%; NT-proBNP, 100%) than for a PCWP ,18 mm Hg (BNP, 42%; NT-proBNP, 60%).. To our knowledge, this study is the first to document such natriuretic peptide-hemodynamic dissociations in the setting of renal failure and suggests that renal function has a more direct effect on circulating natriuretic peptide levels than previously recognized. Moreover, the present study highlights the important limitations of these ...

Background Measurement of plasma concentration of natriuretic peptides (NPs) is suggested to be of value in diagnosis of cardiac disease in dogs, but many factors other than cardiac status may influence their concentrations. Dog breed potentially is 1 such factor. Objective To investigate breed variation in plasma concentrations of pro-atrial natriuretic peptide 31-67 (proANP 31-67) and N-terminal B-type natriuretic peptide (NT-proBNP) in healthy dogs. Animals 535 healthy, privately owned dogs of 9 breeds were examined at 5 centers as part of the European Union (EU) LUPA project. Methods Absence of cardiovascular disease or other clinically relevant organ-related or systemic disease was ensured by thorough clinical investigation. Plasma concentrations of proANP 31-67 and NT-proBNP were measured by commercially available ELISA assays. Results Overall significant breed differences were found in proANP 31-67 (P,.0001) and NT-proBNP (P,.0001) concentrations. Pair-wise comparisons between breeds ...

Cardiac atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) modulate blood pressure and volume by activation of the receptor guanylyl cyclase-A (GC-A) and subsequent intracellular cGMP formation. Here we report what we believe to be a novel function of these peptides as paracrine regulators of vascular regeneration. In mice with systemic deletion of the GC-A gene, vascular regeneration in response to critical hind limb ischemia was severely impaired. Similar attenuation of ischemic angiogenesis was observed in mice with conditional, endothelial cell-restricted GC-A deletion (here termed EC GC-A KO mice). In contrast, smooth muscle cell-restricted GC-A ablation did not affect ischemic neovascularization. Immunohistochemistry and RT-PCR revealed BNP expression in activated satellite cells within the ischemic muscle, suggesting that local BNP elicits protective endothelial effects. Since within the heart, BNP is mainly induced in cardiomyocytes by mechanical load, we investigated ...

We have shown by Boydens chamber method that FCS and PDGF-BB potently stimulate the migration of SMCs. It appears reasonable that FCS induces SMC migration, since blood serum contains numerous growth and migration factors for vascular cells.29 30 Among these factors in serum, PDGF is known to have important roles in the development of atherosclerosis not only as a mitogen but also as a chemoattractant.2 We have confirmed the previous reports3 31 that PDGF-BB, as well as FCS, potently stimulates the migration of SMCs.. Second, we have shown that the natriuretic peptide family, especially CNP, inhibits the migration of SMCs stimulated with FCS and PDGF-BB in a concentration-dependent manner. In addition, when PDGF-BB-induced migration was separated into chemotactic and chemokinetic activities,32 natriuretic peptides strongly suppressed the chemotactic effect of PDGF-BB and modestly suppressed the chemokinetic effect of PDGF-BB. The suppression of the chemotactic effect of PDGF-BB by CNP-22 was ...

Because the thymus expresses the natriuretic peptides (NP) as well as their respective receptors, an involvement of NP in the physiology of this organ has been suggested. To evaluate functional aspects of NP in the thymus, we looked for thymic cells bearing NP receptors (Npr). Furthermore, the regulation of Npr expression by activation of cells and the influence of NP on the proliferation of thymocytes was studied. Expression of receptor messenger RNAs CmRNAs) was examined by PCR and Northern blot. Existence of functional Npr was confirmed by measurement of cGMP, the second messenger of NP. Proliferation of thymocytes upon concanavalin A (Con A) stimulation was analyzed by incorporation of [Hlthymidine. We report here that thymocytes express mRNAs for the three Npr, namely Npra, Nprb, and Nprc and that activation of Npra and Nprb increases cGMP levels. Stimulation of thymocytes with Con A (1 pg/ml, 48 h) resulted in an increase of mRNA coding for Npra, the receptor specific for atria1 ...

Medical term atrial natriuretic factor (ANF) 心房钠尿因子; 心房利尿钠因子 and its variants in English, Simplified Chinese, Traditional Chinese and Pinyin with audio pronunciation. 规范医学术语词汇

Atrial natriuretic peptides (ANP) have recently been identified in both heart and CNS. These peptides possess potent natriuretic, diuretic, and vasorelaxant activities, and are all apparently derived from a single prohormone. Specific ANP binding sites have been characterized in the adrenal zona glomerulosa and kidney cortex, and one study reported ANP binding sites in the CNS. However, a detailed examination of the localization of ANP binding sites throughout the brain has not been reported. In this study, quantitative autoradiography was employed to examine the distribution of ANP receptors in the rat CNS. The binding of (3-125I-iodotyrosyl28) rat ANP-28 to binding sites in the rat CNS was saturable, specific for ANP-related peptides, and displayed high affinity (Kd = 600 pM). When the relative concentrations of ANP binding sites were determined throughout the rat brain, the highest levels of ANP binding were localized to the circumventricular organs, including the area postrema and ...

Mark A. Oyama, Adrian Boswood, David J. Connolly, Stephen J. Ettinger, Philip R. Fox, Sonya G. Gordon, John E. Rush, D. David Sisson, Rebecca L. Stepien, Gerhard Wess, Faiez Zannad, Clinical usefulness of an assay for measurement of circulating N-terminal pro-B-type natriuretic peptide concentration in dogs and cats with heart disease, Journal of the American Veterinary Medical Association, 2013, 243, 1, ...

Results With EA TEA, plasma proANP decreased following induction of anesthesia to the end of surgery (13%; 113±68 to 99±49 pmol/L; p=0.026), but that was not the case in the LA group (3%; 97±44 to 94±49 pmol/L; p=0.565) despite equal fluid balance (+1584±582 vs +1560±563 mL; p=0.888). Accordingly, the EA group required excessive treatment with vasopressors to maintain MAP ,60 mm Hg during surgery (2.7±2 vs 1.6±1.4 ephedrine boluses; p=0.033 and infusion of phenylephrine for 216±86 vs 58±91 min; p,0.001). Plasma proANP and fluid balance were correlated only for EA patients (r=0.44; 95% CI 0.04 to 0.91; p=0.033). ...

Principal Investigator：YAMAMOTO Tadashi, Project Period (FY)：1991 - 1992, Research Category：Grant-in-Aid for General Scientific Research (C), Research Field：Experimental pathology

Background: Chagas disease (CD) affects around 18 million people in Latin America. To determine the diagnostic and prognostic value of natriuretic peptides in patients with CD, we measured atrial (ANP) and brain natriuretic peptide (BNP), and compared the findings with other dilated cardiomyopathies (DCM). Methods: Blood samples were obtained from 111 CD patients, 62 patients with DCM due to other causes, and 43 gender- and age-matched healthy subjects. The CD and DCM patients were subdivided according to their NYHA classification. Natriuretic peptide concentrations were determined by immunoradiometric assays. Results: ANP and more pronounced BNP levels were increased in CD and DCM patients in relation to the NYHA class. Circulating BNP concentrations were higher in CD patients in NYHA classes I-II than in the corresponding DCM patients (p = 0.020). Importantly, ANP and BNP were already significantly elevated in CD patients without systolic ventricular dysfunction (p ≤ 0.001). In CD patients,

In the present study, blood samples were obtained at a single standardized time point, ie, in the morning on the third day of hospitalization. Because of the differences in release and metabolic clearance patterns of the natriuretic peptides, the timing of blood sampling is a crucial issue that may have affected the results of the study. Whether the relative prognostic value of the natriuretic peptides would have differed from the present results had another day of blood collection been selected remains speculative. Until larger studies have been conducted that compare the prognostic value of the natriuretic peptides in blood samples collected at multiple time points in the acute and subacute phase, our data should not be extrapolated to other time points of blood collection. However, the choice of day 3 was based on the following rationale. First, we wanted to choose a time point, based on available data in the literature, that would provide optimal reproducibility. In agreement with the ...

Relationship between natriuretic peptides and echocardiography parameters in patients with poorly regulated type 2 diabetes Magnus Dencker1, Martin Stagmo2, Mozhgan Dorkhan31Unit of Clinical Physiology and Nuclear Medicine, Department of Clinical Sciences, 2Division of Cardiology, 3Division of Diabetes and Endocrinology, Malmö University Hospital, Malmö, SwedenAbstract: This study evaluated the relationship between natriuretic peptide levels and a wide range of echocardiography parameters in a population of thirty-three patients with poorly regulated type 2 diabetes, and no known heart failure. Natriuretic peptides brain natriuretic peptide (BNP) and N-terminal prohormone BNP (NT-proBNP) were measured. Transthoracic echocardiography was performed and cardiac volumes and ejection fraction were measured. Doppler and tissue Doppler were measured and diastolic function was stratified according to recent guidelines. Very few echocardiography parameters were correlated with BNP or NT-proBNP levels.

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the type II transmembrane serine protease class of the trypsin superfamily. Members of this family are composed of multiple structurally distinct domains. The encoded protein converts pro-atrial natriuretic peptide to biologically active atrial natriuretic peptide, a cardiac hormone that regulates blood volume and pressure. This protein may also function as a pro-brain-type natriuretic peptide convertase. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013 ...

Figure 1: Bradykinin metabolism and its relationship with two other vasoactive mediators: angiotensin and natriuretic peptides. ACE, angiotensin-converting enzyme; AI, angiotensin I; AII, angiotensin II; ANP, atrial natriuretic peptide; APP, aminopeptidase P; AT1, angiotensin II type I receptor; AT2, angiotensin II type II receptor; BK, bradykinin; BNP, brain natriuretic peptide; CNP, C-natruretic peptide; NEP, neutral endopeptidase 24.11; NPR-A, natriuretic peptide receptor type A; NPR-B, natriuretic peptide receptor type B ...

Salt-sensitive hypertension is a major risk factor for cardiovascular morbidity. Humans and mice with glucocorticoid receptor haploinsufficiency (GR+/−) are hypertensive, which in mice reflects activation of the renin-angiotensin-aldosterone system. Furthermore, glucocorticoid receptor (GR) gene polymorphisms associate with increased cardiovascular disease risk. Here we investigated the effect of dietary salt intake on blood pressure, heart and kidney in GR+/− mice. Adult male GR+/− mice and wild-type (WT) littermates were fed a sodium-enriched (3%Na+) or standard chow diet (0.3%Na+) for one week, after which mice were anaesthetised for measurement of blood pressure and sodium excretion. Separate cohorts were killed by decapitation and kidneys and hearts taken for histology and mRNA quantification. Cardiac atrial natriuretic peptide (ANP) mRNA levels were elevated in GR+/− mice and were markedly augmented following sodium-enriched diet [Chow:WT 100±7%, GR+/−146±8%; Sodium:WT 157±7%, ...

BACKGROUND: Previous research found precursor levels of the atrial natriuretic peptide (MR-proANP) to be promising prognostic markers. This study aims to validate these findings and describe patterns of MR-proANP in a large cohort of patients with lower respiratory tract infections. METHODS: We conducted a multicenter prospective cohort study, and measured MR-proANP in patients with lower respiratory tract infections on admission, and days 3, 5 and 7. The prognostic value of MR-proANP for predicting 30-day and 180-day mortalities was evaluated. We stratified MR-proANP levels a priori into quartiles, and compared it with severity of illness using the pneumonia severity index. RESULTS: A total of 1359 patients, including 925 with community-acquired pneumonia, were enrolled. The mortality risk at days 30 and 180 significantly increased with increasing MR-proANP quartiles ( 158-311 pmol/L, and ,311 pmol/L). This was true for low-risk, as well as high-risk subjects (pneumonia severity index classes ...