MMP-2 production by tumor cells has been demonstrated to play a fundamental role in ECM degradation and tumor cell invasion (for review see 5 . The recent finding of reduced tumor progression in MMP-2-deficient mice (57) highlights the importance of this molecule. In this study, we wished to determine how MMP-2 gene expression is regulated in human astroglioma cells, as a strong correlation has been observed between astroglioma invasion and MMP-2 expression (17, 19, 20, 22, 23, 27). Our results indicate that two cytokines, TNF-α and IFN-γ, partially inhibit MMP-2 gene expression and can function together in an additive manner for near-complete inhibition of MMP-2 expression in human astroglioma cells. TNF-α/IFN-γ inhibition of MMP-2 expression was observed at several levels: on gelatinolytic activity as determined by zymography, on protein expression (both ProMMP-2 and activated MMP-2) as assessed by immunoblotting, on MMP-2 mRNA expression, and on MMP-2 promoter activity. Our results also ...
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Astrocytomas are a type of cancer of the brain. They originate in a particular kind of glial cells, star-shaped brain cells in the cerebrum called astrocytes. This type of tumor does not usually spread outside the brain and spinal cord and it does not usually affect other organs. Astrocytomas are the most common glioma and can occur in most parts of the brain and occasionally in the spinal cord. Within the astrocytomas, there are two broad classes recognized in literature, those with: Narrow zones of infiltration (mostly noninvasive tumors; e.g., pilocytic astrocytoma, subependymal giant cell astrocytoma, pleomorphic xanthoastrocytoma), that often are clearly outlined on diagnostic images Diffuse zones of infiltration (e.g., high-grade astrocytoma, anaplastic astrocytoma, glioblastoma), that share various features, including the ability to arise at any location in the CNS (Central Nervous System), but with a preference for the cerebral hemispheres; they occur usually in adults; and an intrinsic ...
Background: Immunohistochemical markers are considered as important factors in diagnosis of malignant astrocytomas. The aim of the current study was to investigate the frequency of the immunohistochemical markers neurofilament protein (NFP) and glial fibrillary acidic protein (GFAP) in malignant astrocytoma tumors in Firoozgar and Rasool-Akram hospitals from 2005 to 2010. Materials and Methods: In this cross-sectional study, immunohistochemical analysis of NFP and GFAP was performed on 79 tissue samples of patients with the diagnosisof anaplastic and glioblastoma multiform (GBM) astrocytomas. Results: The obtained results demonstrated that all patients were positive for GFAP and only 3.8% were positive for NFP. There was no significant association between these markers and clinical, demographic, and prognostic features of patients (p|0.05). Conclusions: NFP was expressed only in GBMs and not in anaplastic astrocytomas. It would be crucial to confirm the present findings in a larger number of tumors,
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A case of anaplastic pleomorphic xanthoastrocytoma (PXA) in a 9-year-old girl is reported. Histological features of PXAs are cellular pleomorphism of GFAP-positive cells, with intracytoplasmic lipidic vacuoles and a reticulin network, bizarre giant cells, low mitotic activity, and lack of necrosis and of endothelial vascular proliferations. These tumors are generally reported to have a favorable postoperative course. In our case, a poor clinical prognosis and spread of the illness through the CSF was observed. Immunohistochemical features of the tumor, which were histologically anaplastic in nature, were analyzed. There were small foci of necrosis in the sections of the material obtained at the first operation and extensive necrosis in that from the second operation, although the patient had not received radiotherapy between the operations. The presence of necrosis in PXA is an uncommon and significant feature. It predicts the poor prognosis seen in this case, and therefore this report strongly supports
Anaplastic astrocytoma is a rare WHO grade III type of astrocytoma, which is a type of cancer of the brain. In the United States, the annual incidence rate for Anaplastic astrocytoma is 0.44 per 100,000 persons Anaplastic astrocytomas fall under the category of high grade gliomas (WHO grade III-IV), which are pathologically undifferentiated gliomas that carry a poor clinical prognosis. Unlike glioblastomas (WHO grade IV), anaplastic astrocytomas lack vascular proliferation and necrosis on pathologic evaluation. Compared to grade II tumors, anaplastic astrocytomas are more cellular, demonstrate more atypia, and mitoses are seen. Initial presenting symptoms most commonly are headache, depressed mental status, focal neurological deficits, and/or seizures. The growth rate and mean interval between onset of symptoms and diagnosis is approximately 1.5-2 years but is highly variable, being intermediate between that of low-grade astrocytomas and glioblastomas. Seizures are less common among patients ...
TY - JOUR. T1 - Adult Pilocytic Astrocytoma. T2 - An Institutional Series and Systematic Literature Review for Extent of Resection and Recurrence. AU - Bond, Kamila M.. AU - Hughes, Joshua D.. AU - Porter, Amanda L.. AU - Orina, Josiah. AU - Fang, Shanna. AU - Parney, Ian F.. PY - 2018/2/1. Y1 - 2018/2/1. N2 - Introduction Pilocytic astrocytoma is a classically benign tumor that most often affects pediatric patients. Rarely, it occurs during adulthood. We present a case series and systematic literature review of adult pilocytic astrocytoma (APA) to examine the clinical presentation, extent of resection, and recurrence rate associated with this tumor in this population. Materials and Methods Our institutional records were retrospectively reviewed for cases of pilocytic astrocytoma in adults. A PubMed search identified English-language studies of pathology-proven APA. A meta-analysis was performed to determine the relationship between extent of tumor resection and recurrence. Results Forty-six ...
TY - JOUR. T1 - An elderly case of malignant small cell glioma with hemorrhage coexistent with a calcified pilocytic astrocytoma component in the cerebellar hemisphere. AU - Sangatsuda, Yuhei. AU - Hata, Nobuhiro. AU - Suzuki, Satoshi O.. AU - Akagi, Yojiro. AU - Hatae, Ryusuke. AU - Kuga, Daisuke. AU - Yoshimoto, Koji. AU - Momosaki, Seiya. AU - Iwaki, Toru. AU - Iihara, Koji. PY - 2018/1/1. Y1 - 2018/1/1. N2 - Pilocytic astrocytoma is a less aggressive form of glial tumor that commonly occurs in the pediatric population, and its malignant transformation is extremely rare. Here, we report an elderly case of malignant small cell glioma with hemorrhage coexistent with a calcified pilocytic astrocytoma component. An 80-year-old male was found to have a right cerebellar non-enhanced tumor with hematoma adjoining a calcified nodule. The lesion was surgically removed, and a histological examination verified that the tumor was a malignant small cell glioma with hemorrhagic change and the calcified ...
buffalogirl - Survivor: Brain and Spinal Cord Tumors in Adults > Astrocytoma > High-Grade Astrocytoma (also called Glioblastoma) Patient Info: Finished active treatment less than 5 years ago, Diagnosed: over 12 years ago, Female, Age: 53
ljl4mj - Lost Loved One: Brain and Spinal Cord Tumors in Adults > Astrocytoma > High-Grade Astrocytoma (also called Glioblastoma) Patient Info: Diagnosed: over 8 years ago, Female
TY - JOUR. T1 - Prognostic significance of multiple kallikreins in high-grade astrocytoma. AU - Drucker, Kristen L.. AU - Gianinni, Caterina. AU - Decker, Paul A.. AU - Diamandis, Eleftherios P.. AU - Scarisbrick, Isobel A. PY - 2015/8/1. Y1 - 2015/8/1. N2 - Background: Kallikreins have clinical value as prognostic markers in a subset of malignancies examined to date, including kallikrein 3 (prostate specific antigen) in prostate cancer. We previously demonstrated that kallikrein 6 is expressed at higher levels in grade IV compared to grade III astrocytoma and is associated with reduced survival of GBM patients. Methods: In this study we determined KLK1, KLK6, KLK7, KLK8, KLK9 and KLK10 protein expression in two independent tissue microarrays containing 60 grade IV and 8 grade III astrocytoma samples. Scores for staining intensity, percent of tumor stained and immunoreactivity scores (IR, product of intensity and percent) were determined and analyzed for correlation with patient survival. ...
Astrocytes are the most common type of cell in the central nervous system. They perform many essential roles and make up the supportive tissue of the brain. When there is a defect in an astrocyte that causes it to grow out of control, the brain tumor that forms is called an astrocytoma.. Astrocytomas come in four major subtypes: pilocytic astrocytoma (grade 1), fibrillary astrocytoma (grade 2), anaplastic astrocytoma (grade 3), and glioblastoma multiforme (grade 4).. Low-grade astrocytomas (grades 1 and 2), seen more often in kids and young adults, are highly curable because they usually grow slowly, dont spread, and usually are fairly easy to remove unless found in areas where surgery would be difficult. After surgery, theres a chance that chemotherapy or radiation wont be needed.. High-grade astrocytomas (grades 3 and 4), which tend to be seen more in adults, are more aggressive and invade deeper into the tissues. This makes them more difficult to treat. Treatment usually includes surgery, ...
Presence of IDH1 mutation determines best treatment for malignant astrocytomas. Astrocytomas are the most common malignant brain tumors. While most patients tumors prove to be quite aggressive, outcomes overall can vary widely, with some patients surviving for many years. Now a new study has found that malignant astrocytoma patients whose tumors carry a specific genetic mutation benefit greatly from surgical removal of the largest possible amount of tumor. Preliminary results of the study were reported at the 2012 American Society of Clinical Oncology meeting, and the teams full report appears in the January issue of the journal Neuro-Oncology. A type of glioma, astrocytomas include the highly aggressive glioblastoma and the less aggressive but still dangerous anaplastic astrocytoma.. "We found that the benefit of surgery and how aggressively the surgery should be done depend, in large part, on whether or not patients tumors have the mutated form of the IDH1 gene," says Daniel Cahill, MD, ...
Malignant astrocytomas, which are highly invasive, vascular neoplasms, compose the majority of nervous system tumors in humans. Elevated expression of fibroblast growth factors (FGFs) in astrocytomas has implicated the FGF family of mitogens in the initiation and progression of astrocyte-derived tumors. In this study, we demonstrated that human astrocytomas undergo parallel changes in FGF-receptor (FGFR) expression during their progression from a benign to a malignant phenotype. FGFR type 2 (BEK) expression was abundant in normal white matter and in all low-grade astrocytomas but was not seen in malignant astrocytomas. Conversely, FGFR type 1 (FLG) expression was absent or barely detectable in normal white matter but was significantly elevated in malignant astrocytomas. Malignant astrocytomas also expressed an alternatively spliced form of FGFR-1 (FGFR-1 beta) containing two immunoglobulin-like disulfide loops, whereas normal human adult and fetal brains expressed a receptor form (FGFR-1 alpha) ...
Background Astrocytomas are the most aggressive and common human brain tumors characterized by their highly invasive development. of miR-335 in C6 cells enhances cell viability significantly, colony-forming invasiveness and ability. Conversely, delivery of villain particular for miR-335 (antagomir-335) to C6 cells outcomes in development police arrest, cell apoptosis, attack dominance and proclaimed regression of astrocytoma xenografts. Additional analysis reveals that miR-335 focuses on disheveled-associated activator of morphogenesis 1(Daam1) at posttranscriptional level. Furthermore, silencing of endogenous Daam1 (siDaam1) could imitate the oncogenic results of miR-335 and invert the development police arrest, proapoptotic and attack dominance results caused by antagomir-335. Particularly, the oncogenic results of miR-335 and siDAAM1 collectively with anti-tumor results of antagomir-335 are also verified in human being astrocytoma U87-MG cells. Summary These results recommend an oncogenic ...
Mitochondrial Lactate Dehydrogenase Is Involved in Oxidative-Energy Metabolism in Human Astrocytoma Cells CCF-STTG1. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
The cyclin-dependent kinase member, Cdk5, is expressed in a variety of cell types, but neuron-specific expression of its activator, p35, is thought to limit its activity to neurons. Here we demonstrate that both Cdk5 and p35 are expressed in the human astrocytoma cell line, U373. Cdk5 and p35 are present in the detergent-insoluble cytoskeletal fraction of this cell line and Cdk5 localizes to filopodia and vinculin-rich regions of cell-matrix contact in lamellopodia. When exposed to a 46(o)C heat shock, U373 cells change shape, lose cell-matrix contacts and show increased levels of apoptosis. To test whether Cdk5 activation might play a role in these events, U373 cells were stably transfected with histidine-tagged or green fluorescent protein-tagged constructs of Cdk5 or a dominant negative mutation, Cdk5T33. Under normal growth conditions, growth characteristics of the stably transfected lines were indistinguishable from untransfected U373 cells and Cdk5 localization was not changed. However, ...
Work in this thesis was primarily involved with the characterisation of four receptors, the turkey and bovine P2Y1, and the human P2Y2 and P2Y4 receptors, transfected into the human astrocytoma cell line, 1321N1. It also describes the preliminary characterisation of two vascular smooth muscle cell types, and the generation of P2Y1-GST fusion proteins for the production of antibodies.;1321N1 cells were found to release nucleotides in response to sheer stress, but this was overcome by adaptation of the stimulation method. 1321N1 cells expressing the four receptors were assayed for PLC activity, and the rank order of agonist potency for each receptor was generally consistent with reports in the literature. Nucleotide interconversion at the surface of 1321N1 cells was also examined indirectly using the ecto-ATPase inhibitor ARL 67156 and hexokinase. Although there was no significant effect of nucleotide breakdown on the agonist concentration-responses, it was found that upstream conversion of ...
Low-grade astrocytomas are a heterogeneous group of intrinsic central nervous system (CNS) neoplasms that share certain similarities in their clinical presentation, radiologic appearance, prognosis, and treatment. The most common intrinsic brain tumor, glioblastoma multiforme, is high grade and malignant.
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For patient information click here Editor-In-Chief: C. Michael Gibson, M.S., M.D. [1];Associate Editor(s)-in-Chief: Marjan Khan M.B.B.S.[2] Synonyms and Keywords: Spongioblastoma multiforme; glioblastoma; glioblastomas; GBM; GBMs; G.B.M; grade IV astrocytoma; grade IV astrocytomas; astrocytoma, grade IV; undifferentiated glioma; grade IV malignant astrocytoma; grade IV malignant glioma; kernohan grade IV astrocytoma; St. Anne/Mayo astrocytoma grade 4; butterfly glioma ...
Intramedullary mixed signal cervical spinal cord mass. No evidence of hemorrhage. Mild expansile remodeling of the cervical spinal bony canal. The tumor enhances heterogeneously
In the present series, glioma was the most common diagnosis, representing 40.1% of cases (sixty nine patients): 23 (33.8%) were classified as glioblastoma multiforme (WHO IV), 13 (19.1%) as anaplasic astrocytoma (WHO III) and 11 as low grade astrocytomas (10%). Two patients (1.2%) had their lesions classified as probable low grade astrocytoma and one (0.6%) as oligodendroglioma.. The rest of tumors (12.2%) were classified as: lymphomas (7%), metastasis (2.3%), germinomas (1.2%), epidermoid cyst (0.6%) and primitive neuroectoderm tumor (1.2%). Metastasis were originated from pulmonary carcinoma, kidney and colon. Only one case ended in undifferentiated carcinoma as the diagnosis. Two HIV positive patients had B-cell non-Hodgkin lymphomas. From the other 10 HIV negative patients, 5 had the same B-cell non-Hodgkin lymphoma, 1 T-cell lymphoma, 2 high grade non-Hodgkin lymphoma, 1 primary brain lymphoma and 1 a Reed- Steinberg variance of Hodgkins disease.. An inflammatory or infectious process was ...
Progesterone receptors (PR) have been detected in human astrocytomas; however, the expression pattern of PR isoforms in these brain tumors is unknown. Progesterone receptor isoforms expression was studied in 13 biopsies of astrocytomas (6 grade III, and 7 grade IV) from adult Mexican patients by using reverse transcription-polymerase chain reaction and immunohistochemistry. Progesterone receptor expression was observed at mRNA and at protein levels in 66% and 83% of astrocytomas grade III, respectively, whereas 100% of astrocytomas grade IV expressed PR. Almost all PR mRNA content in astrocytomas grades III and IV corresponded to PR-B. The number of immunoreactive cells expressing PR-B was higher than that expressing PR-A in 73% of the cases. Estrogen receptor-á protein was only observed in 33% of astrocytomas grade III, whereas no astrocytomas grade IV expressed it. These data suggest that PR-B is the predominant isoform expressed in human astrocytomas grades III and IV, and that estrogen ...
Adjuvant chemotherapy using DNA-damaging agents has largely failed to make a significant impact on the outcome of patients with malignant astrocytoma. One of the primary mechanisms of resistance to nitrosureas such as CCNU is mediated through O6-methylguanine-DNA methyltrans-ferase (MGMT). This DNA repair enzyme removes the cytotoxic alkyl adducts from O6-guanine, and hence the level of MGMT activity in tumor cells is related to their sensitivity to nitroureas. It has been proposed that functional inactivation of MGMT through hypermethylation of the gene promotor region could be predictive of chemosensitivity. We have previously reported differential sensitivity to CCNU in a panel of 17 short-term cultures derived from malignant astrocytoma. In this study, we determined the methylation status of MGMT using methylation-specific PCR in these 17 cultures. We also assessed the amounts of MGMT mRNA and protein present in each culture using real-time quantitative PCR and immunohistochemistry with a ...
MR diffusion imaging has been used to study water mobility in normal brain tissue (1, 2), cerebral infarction (2), multiple sclerosis (3), gliomas (4-8), and brain abscesses (8, 9) and to differentiate between arachnoid cysts and epidermoid cysts (10) and other diseases (7, 8, 13, 14). Gliomas are the most common brain tumors. On imaging studies, malignant gliomas usually are enhanced after intravenous contrast injection and show peritumoral edema, whereas, except for pilocytic astrocytoma and giant-cell astrocytoma, low-grade gliomas usually show little to no abnormal enhancement or peritumoral edema. Differentiation of these two types of tumors occasionally may be difficult, because low-grade astrocytomas also may show abnormal contrast enhancement and peritumoral edema. In fact, abnormal enhancement was noted in four of eight patients with grade II astrocytoma in our study, and peritumoral edema was found in four patients.. We found that ADC values cannot be used in individual cases to ...
Astrocytic tumors are the most common intracranial neoplasms. Their prognoses correlate with a conventional morphological grading system that suffers from diagnostic subjectivity and hence, inter-observer inconsistency. A molecular marker that provides an objective reference for classification and prognostication of astrocytic tumors would be useful in diagnostic pathology. RhoA, a GTPase protein involved in cell migration and adhesion, has been shown to be upregulated in a variety of human cancers. Based on direct analysis of clinical materials, our study demonstrates increased expression of RhoA in high-grade astrocytomas. This observation may be relevant to astrocytoma biology and the development of potential therapeutics against high-grade astrocytomas. Of more immediate consequence, utilization of this marker may aid in the routine pathological grading (and hence prognostication) of astrocytomas. © 2006 Elsevier Ireland Ltd. All rights reserved ...
In bacterial meningitis, LPS induces production in cerebrospinal fluid of the cytokines IL-1 beta and tumor necrosis factor alpha (TNF alpha), which are the principle mediators of meningeal inflammation. IL-1 beta and TNF alpha induce fever, and elevated temperature may affect cytokine expression. Dexamethasone treatment improves outcome in bacterial meningitis possibly by inhibiting IL-1 beta and TNF alpha. In this report, the effects of elevated temperature and dexamethasone on LPS-stimulated IL-1 beta and TNF alpha mRNA gene expression and protein synthesis were studied in human astrocytoma cell lines and primary cultures of human fetal astrocytes. Cells cultured at 40 degrees C exhibited smaller peaks of IL-1 beta and TNF alpha transcription and protein synthesis compared with cells cultured at 37 degrees C. The addition of dexamethasone before, during, or after exposure of the cells to LPS resulted in temperature-dependent inhibition of IL-1 beta transcription and protein synthesis. The ...
Klironomos, G., Bravou, V., Papachristou, D. J., Gatzounis, G., Varakis, J., Parassi, E., Repanti, M. and Papadaki, H. (2010), Loss of Inhibitor of Growth (ING-4) is Implicated in the Pathogenesis and Progression of Human Astrocytomas. Brain Pathology, 20: 490-497. doi: 10.1111/j.1750-3639.2009.00325.x ...
This never-ending cycle of tests, treatments and time spent recovering from surgeries deprives diagnosed children of their childhood. We have watched our son suffer through this disease for almost 9 years. Most devastating of all, we could not stop the tumor from taking his eyesight.. ...
Protoplasmic astrocytoma is a rare variant of diffuse low-grade astrocytomas with histological and imaging features which overlap with other entities. Until recently they were classified as a subtype of low-grade diffuse astrocytoma, however, i...
A collection of disease information resources and questions answered by our Genetic and Rare Diseases Information Specialists for Cerebral astrocytoma, childhood
Neuroepithelial tumors. Astrocytic tumors. An increased risk of astrocytic tumors has been observed in patients who receive therapeutic radiation therapy for pituitary adenomas, craniopharyngioma, pineal parenchymal tumors, germinoma, and tinea capitis. In addition, children who receive prophylactic radiation therapy of the CNS for acute lymphoblastic leukemia have an increased risk of developing astrocytomas. Recurrent lesions often signal histologic progression to a higher grade; this malignant progression is associated with a cumulative acquisition of multiple genetic alterations. [4] Pilocytic astrocytoma (WHO grade I) is a grossly circumscribed, slow-growing, often cystic tumor that occurs primarily in children and young adults. [5] Histologically, pilocytic astrocytomas are composed of varying proportions of compacted bipolar cells with Rosenthal fibers and loose-textured multipolar cells with microcysts and granular bodies. This tumor is the most common glioma in children and represents ...
Introduction:. Astrocytomas are the most common subtype of glioma, accounting for about 70% of all malignant tumors found in the central nervous system. Genetic alterations in gliomas have been extensively researched, but epigenetic changes are less well investigated. The aim of this study is to analyze genome-wide patterns of DNA methylation in a set of astrocytic tumors to identify aberrantly methylated genes, which could serve as candidates for new diagnostic markers and/or therapeutic targets.. Material & Methods:. DNA methylation was investigated genome-wide by methyl-DNA immunoprecipitation (MeDIP) and hybridization to customized Agilent Human CpG island (250K) oligonucleotide arrays. In total, 18 glioblastoma cell lines, 59 astrocytic tumor samples (4 diffuse astrocytomas WHO grade II, 17 anaplastic astrocytomas grade III and 38 glioblastomas grade IV) and 5 normal whole brain samples were analyzed. Data quality control, normalization and analysis was performed through the arrayQuality ...
Contrast studies -- Coronal post-gadolinium T1-weighted image shows the medial portion of solid component enhancing with contrast material. Note bilateral tonsils sagging into the ...
My husband was diagnosed with what we call a family nightmare brain tumor call Anaplastic Astrocytoma Grade 3 this past year end on October 2012 He had hardly no symtoms just some tingling on his left hand which he thought to be arthritis in his joints and suddenly developed a fever over night mid October we went to the hospital where we were told it was the flu but what we thought to be the flu as quick as it came it returned my husband left home seemingly fine gave me a kiss and went off to work driving through midtown Manhattan he suddenly felt what seem to be a charlie horse pain in his left cuff and promptly parked his plumbing truck vehicle which he drove while his co-worker watched and followed behind him to the side of the vehicle when he calpse to the ground what it look like a stroke was 5 seizures by the time he arrived at New York Presbyterian Cornell ER many seris of test done Blood/Urine Test, Cat Scan, X Ray and finally MRI they found he had a mass in an inoperable location in the ...
C4d is a cleavage product of complement component C4 and is considered to serve as a marker for the site of complement activation. In this study C4d staining of grade I-IV astrocytic tumors was studied to explore if there is an association between complement activation and the grade of tumor, or patient survival. Tissue micro-array samples of 102 astrocytomas were stained immunohistochemically. The material consisted of 9 pilocytic astrocytomas and 93 grade II-IV astrocytomas, of which 67 were primary resections and 26 recurrent tumors. The intensity of C4d staining as well as extent of C4d and CD34 staining were evaluated. The intensity of C4d staining was scored semiquantitatively. The extent of the staining was counted morphometrically with a point counting grid yielding a percent of C4d and CD34 positive area of the sample. The intensity and extent of C4d staining increased in grade II-IV diffusely infiltrating astrocytoma tumors in line with the malignancy grade (p = 0.034 and p = 0.016,
Phase 2 trial to explore the efficacy and safety of irinotecan (CPT-11) in patients with recurrent anaplastic astrocytomas (AA), mixed malignant glioma, and oligodendrogliomas (OA). Patients were to be stratified by tumor histology and treated with CPT-11 every 21 days (treatment cycle).. Baseline data (collected ,14 days) was to consist of a neurological/oncological history, neurological examination, height, weight, performance status, Quality Of Life FACT-L questionnaire, laboratory studies to include complete blood count (CBC), differential, platelets, prothrombin time (PT), complete metabolic panel (CMP), Lactose dehydrogenase (LDH), and a pregnancy test, as well as a cranial Computerized Tomography/Magnetic Resonance Imaging (CT/MRI) with and without contrast (to measure or evaluate the size and location of the tumor before treatment).. Administered every 21 days was a dose of irinotecan (CPT-11), zofran/Kytril/Anzemet, decadron, and intravenous (IV) atropine. At each cycle, patient exams ...
Phase 2 trial to explore the efficacy and safety of irinotecan (CPT-11) in patients with recurrent anaplastic astrocytomas (AA), mixed malignant glioma, and oligodendrogliomas (OA). Patients were to be stratified by tumor histology and treated with CPT-11 every 21 days (treatment cycle).. Baseline data (collected ,14 days) was to consist of a neurological/oncological history, neurological examination, height, weight, performance status, Quality Of Life FACT-L questionnaire, laboratory studies to include complete blood count (CBC), differential, platelets, prothrombin time (PT), complete metabolic panel (CMP), Lactose dehydrogenase (LDH), and a pregnancy test, as well as a cranial Computerized Tomography/Magnetic Resonance Imaging (CT/MRI) with and without contrast (to measure or evaluate the size and location of the tumor before treatment).. Administered every 21 days was a dose of irinotecan (CPT-11), zofran/Kytril/Anzemet, decadron, and intravenous (IV) atropine. At each cycle, patient exams ...
Grade 2 to 4 pediatric astrocytomas share histopathologic similarities with their corresponding adult counterparts, and, in general, pathologic examination does not distinguish pediatric and adult tumors of the same malignancy grade. Numerous studies have identified signature genetic mutations in high-grade adult astrocytomas, including the recent TCGA analysis of GBM tumors (4). The TCGA study showed that EGFR, PTEN, and TP53 changes are among the most frequently observed alterations in adult GBM, whereas recent studies using parallel genomic approaches showed that mutations in the IDH1 gene predominate in adult gliomas (5, 16). With the possible exception of TP53 mutation, the frequent genetic alterations seen in adult astrocytomas have been identified at lower frequencies in pediatric astrocytomas (2, 3, 17). This lack of signature genetic mutations in pediatric astrocytomas is unfortunate, as these serve as important reference points with which to test therapeutic hypotheses.. The results of ...
About one-third of brain tumors are diffuse astrocytic tumors associated with dismal prognoses because of their marked capacity to diffusely invade the normal brain parenchyma. Their level of malignancy increases from astrocytomas (World Health Organization/WHO grade II) to anaplastic astrocytomas (WHO grade III) and glioblastoma (WHO grade IV). The human U373 cellular model used in our experiments exhibited biological and histopathological properties characteristic of a malignant astroglioma. The exceptional capacity of isolated astrocytic tumor cells to diffuse from the tumor body into the normal surrounding brain parenchyma is due to two major events, namely, invasion and motility (De Hauwer et al., 1998).. Although no effective treatment exists today against malignant astrocytomas, and because we observed that gastrin is able to significantly modulate the cell growth and motility of human glioblastoma cells (Camby et al., 1996; De Hauwer et al., 1998), we believe that gastrin-based therapy ...
Canada, and more specifically its Brain Tumour Foundation, has decided to fall in line with the Americans again: May is the new Brain Tumour Awareness Month. So much for the Holy Month of October (although I do very much respect the mammary aspect of that month). I shant fountainously spout my bile over the various little nitty-picky problems I have with this example of Manifest Destiny, assuming the spellings of grey and tumour shall not change to the rather appalling gray and tumor, and instead I choose to simply celebrate my "own" tumour day. For according to the Foundations Brain-Tumour-a-Day-in-May schedule, the 18th of May is reserved for Anaplastic Astrocytoma. Very well. It would have been, once upon a time, the 4th of May: Oligoastrocytoma day. Well, grade II no more. Onwards. ...
The relative resistance of malignant glioma to chemotherapy makes the identification of new cytotoxic drugs critically important. The use of short-term cultures derived from these tumors to screen drugs at doses that can be attained within human intracranial tumors provides a model system that should be capable of identifying effective drugs suitable for clinical evaluation. The sensitivity of a panel of short-term cultures derived from 22 malignant astrocytoma and four malignant oligodendroglioma was assessed to aziridinylbenzoquinone (AZQ), etoposide and doxorubicin (DOX) using a [(35)S] methione uptake assay. The ID(50) of each culture was compared to the levels of drug which could be achieved in the tumor using standard doses. There was marked heterogeneity between cultures in response to each drug. Whilst there was no evidence that cultures derived from grade III astrocytoma were more sensitive to any of the drugs than cultures derived from grade IV astrocytoma, cultures derived from ...
Astrocytic tumors are the most common malignancies of the central nervous system, accounting for more than 60% of all primary brain tumors. The prognosis for high grade astrocytoma patients is dismal and there is no curative treatment, today. A molecular hallmark of astrocytic tumors is dysregulated receptor tyrosine kinase signaling, especially of the epidermal growth factor receptor (EGFR, ErbB1). The aim of the present thesis was to identify endogenous human proteins that downregulate the function of the ErbB1 receptor. We identified a human gene family, the leucine-rich repeats and immunoglobulin-like domains family (LRIG), consisting of LRIG1, LRIG2 and LRIG3, which might fulfill this criterion.. Two candidates were identified, LRIG1 and LRIG2, which genes were localized to regions frequently deleted in human cancers, chromosome bands 3p14 and 1p13, respectively. LRIG1 and LRIG2 mRNA were expressed in all tissues analyzed, with high expression in brain and other organs. The LRIG mRNA were ...
Astrocytic tumors are the most common malignancies of the central nervous system, accounting for more than 60% of all primary brain tumors. The prognosis for high grade astrocytoma patients is dismal and there is no curative treatment, today. A molecular hallmark of astrocytic tumors is dysregulated receptor tyrosine kinase signaling, especially of the epidermal growth factor receptor (EGFR, ErbB1). The aim of the present thesis was to identify endogenous human proteins that downregulate the function of the ErbB1 receptor. We identified a human gene family, the leucine-rich repeats and immunoglobulin-like domains family (LRIG), consisting of LRIG1, LRIG2 and LRIG3, which might fulfill this criterion.. Two candidates were identified, LRIG1 and LRIG2, which genes were localized to regions frequently deleted in human cancers, chromosome bands 3p14 and 1p13, respectively. LRIG1 and LRIG2 mRNA were expressed in all tissues analyzed, with high expression in brain and other organs. The LRIG mRNA were ...
SUAREZ, Julio César et al. Gliomas cerebrales de bajo grado en el adulto. Rev. argent. neurocir. [online]. 2008, vol.22, n.1. ISSN 1850-1532.. Objective. Gliomas reviewed in this article are grade II tumors according to the World Health Organization (WHO), that include: fibrillary and protoplasmic astrocytomas, oligodendrogliomas and oligoastrocytomas or mix tumors (1,2,3).Low grade astrocytomas constitute 15% of brain tumors in adults, while low grade oligodendrogliomas represent 4% (2,4). We present our experience with this type of tumor operated on between January 1972 and December 2006. Material and Method. The clinical reports of 25 patients with this type of tumor were analyzed, 15 women and 10 men, which represent 15,6% of hemispheric brain gliomas in adults in our series. Results. Fifteen were fibrillary astrocytomas, 8 oligodendrogliomas and 2 oligoastrocytomas. Treatment depended on tumor localization and size. Surgery and radiotherapy were the therapeutic modalities most frequently ...
4Mustafa Kemal University, Faculty of Medicine, Department of Neurosurgery, Hatay, Turkey DOI : 10.5137/1019-5149.JTN.5347-11.3 AIM: Retrospective investigation of prognostic factors in low-grade astrocytomas.. MATERIAL and METHODS: In the study, prognostic factors were prospectively identified and assumed to be effective on prognosis, which were reviewed in 67 cases diagnosed as low-grade astrocytoma with craniotomy between May 1998 to December 2005 at Erciyes University Neurosurgery Department. Assessment of demographic, neurologic, radiological, surgical and clinical features of cases and adjuvant therapies and their relationship with prognosis were evaluated. Post-operative cumulative survival of the cases was estimated by using the Kaplan-Meier method. The Log Rank test was used to compare the survival curve of the sub-groups. Multivariate analysis of survival was analyzed by using the Cox regression method.. RESULTS: It was seen that malign transformation occurred in 9 patients of subtotal ...
TY - JOUR. T1 - Involvement of aquaporin in thromboxane A2 receptor-mediated, G12/13/RhoA/NHE-sensitive cell swelling in 1321N1 human astrocytoma cells. AU - Saito, Masaki. AU - Tanaka, Hiroyuki. AU - Sasaki, Masako. AU - Kurose, Hitoshi. AU - Nakahata, Norimichi. PY - 2010/1/1. Y1 - 2010/1/1. N2 - The physiological role of the thromboxane A2 (TXA2) receptor expressed on glial cells remains unclear. We previously reported that 1321N1 human astrocytoma cells pretreated with dibutyryl cyclic AMP (dbcAMP) became swollen in response to U46619, a TXA2 analogue. In the present study, we examined the detailed mechanisms of TXA2 receptor-mediated cell swelling in 1321N1 cells. The cell swelling caused by U46619 was suppressed by expression of p115-RGS, an inhibitory peptide of Gα12/13 pathway and C3 toxin, an inhibitory protein for RhoA. The swelling was also inhibited by treatment with Y27632, a Rho kinase inhibitor and 5-(ethyl-N-isopropyl)amiloride (EIPA), a Na+/H+-exchanger inhibitor. Furthermore, ...