Here, we have synthesized the silver nanoparticles (AgNPs) by using the soil fungus Aspergillus niger 2587. The results recorded from UV-vis spectrophotometer and transmission electron microscopy (TEM) support the biosynthesis and characterization of AgNPs. The synthesized silver nanoparticles have also been tested against the larvae and pupae of Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti. The efficacy test was performed at different concentrations for a period of different hours by the probit analysis. The larvae of Cx. quinquefasciatus have shown the 100% mortality to the synthesized AgNPs after 1 h of exposure, while the larvae of An. stephensi and Ae. aegypti were found less susceptible to the synthesized AgNPs. The pupa of Ae. aegypti has shown the efficacy LC50 4, LC90 12 and LC99 19 ppm after 2 h of exposure of the synthesized AgNPs, while, the pupae of Cx. quinquefasciatus and An. stephensi were found less susceptible to the synthesized AgNPs. By this approach, it is
The filamentous fungus Aspergillus niger was transformed with the hepatitis B virus S gene encoding the major viral envelope protein under control of the constitutive A. nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) promoter. Approximately seven copies of the expression cassette were integrated on the genome, resulting in high-level transcription of the S gene. Production of the 24-kDa S protein and a 48-kDa S protein dimer in the membrane-associated protein fraction of the recombinant A. niger strain was shown through Western analysis. Electron microscopy of partially purified recombinant S protein revealed the formation of spherical pseudoviral particles with a diameter of 22 nm. The production level of hepatitis B pseudoviral particles was estimated to be 0.4 mg/1 culture, which compares favourably with the reported levels initially obtained in yeast, indicating the potential of the Aspergillus expression system as an alternative, cost-effective vaccine production system.. ...
Aspergillus niger ATCC ® 1015D-2™ Designation: Genomic DNA from Aspergillus niger strain 3528.7 [ATCC ® 1015™] Application:
A recombinant Aspergillus niger strain expressing the Hypocrea jecorina endoglucanase Cel7B was grown on spent hydrolysates (stillage) from sugarcane bagasse and spruce wood. The spent hydrolysates served as excellent growth media for the Cel7B-producing strain, A. niger D15[egI], which displayed higher endoglucanase activities in the spent hydrolysates than in standard medium with a comparable monosaccharide content (e.g., 2,100 nkat/ml in spent bagasse hydrolysate compared to 480 nkat/ml in standard glucose-based medium). In addition, A. niger D15[egI] was also able to consume or convert other lignocellulose-derived compounds, such as acetic acid, furan aldehydes, and phenolic compounds, which are recognized as inhibitors of yeast during ethanolic fermentation. The results indicate that enzymes can be produced from the stillage stream as a high-value coproduct in secondgeneration bioethanol plants in a way that also facilitates recirculation of process water. Copyright © 2009, American ...
Aspergillus niger ATCC ® 11414™ Designation: A-1-233 [72-4, A60, IMI 75353, NRRL 2270, VTT D-77050] Application: degrades apple pomace produces 6-phosphofructo-2-kinase produces ATP citrate lyase produces aconitic hydrase produces carnitine acetyltransferase produces citric acid citrate produces polygalacturonase endopolygalacturonase, pectinase transformation host produces citric acid from apple pomace
A rapid growing and high xylanase producing fungus Aspergillus niger SA7, was isolated from starch waste. The best yield of xylanase was 2400 U/g on the solid-state starch waste with 65-70% of initial moisture content at 28℃ for 3 days. Crude enzyme preparation contained more components of cellulase. Addition wheat bran to medium could stimulated xylanase production. 21.6% reducing sugar was obtained by hydrolysis starch waste at 45℃ culture for 24 hours.
Microorganisms generally encounter a fluctuating environment in their natural habitat and similar conditions also happen in large-scale bioreactors. In this work, the dynamic response of intracellular and extracellular metabolites of Aspergillus niger was investigated after sudden exposure to high and low excess glucose concentrations in chemostats. It was found that the steady-state pathway turnover time of the carbon flux through the central carbon metabolism (CCM) was PP pathway 50 s, EMP pathway 20 s, and TCA cycle 189 s, and an upper limit for individual metabolite concentrations in the CCM was estimated. Regardless of the glucose pulse size, little changes of amino acids levels were observed except for aspartate, which showed a significant decrease. The ATP paradox, known from other organisms, was also observed in the studied A. niger strain. However, a different response of the NAD+/NADH ratio to the glucose pulses was found in A. niger compared to previously published observations on Penicillium
Triticale (x Triticosecale, Wittmack), an important industrial crop, with high grain yield, containing high amounts of starch, proteins and also major and minor mineral elements, is not yet sufficiently utilized. The simultaneous production of alpha-amylase and glucoamylase isoforms by Aspergillus niger on triticale grains, without any nutritive supplements, was developed, optimized and scaled up 10 fold for the first time. The specific combination of the examined effects led to the production of a novel glucoamylase isoform. Reduction of particle size, increase in oxygen availability and substrate height lead to an increase of 30 % in the production of amylases. Reduction of the relative humidity from 65 to 30 % increased glucoamylase production 2 fold and alpha-amylase production by 30%. The peak production of alpha-amylase (158 U g(-1)) and glucoamylase (170 U g(-1)) were obtained in Erlenmeyer flasks and in scaled-up trays. The obtained A. niger amylase cocktail was more efficient ...in raw ...
Dr. Chenyu Du has more than 15 years research experience in fermentation, metabolic engineering, bioprocess development and simulation, bio-separation and biomass pretreatment. He has published 30+ high-impact publications, including 30+ papers, 2 patents and 5 book chapters. Since joined the University of Nottingham, he generated research income over £570 k as PI (including fully funded PhD studentships), £14.3 m as Co-PI and supervised, co-supervised 10 PhD students, 2 MRes students, 15+ MSc students and 15+ undergraduates. At the University of Nottingham, Chenyu has been involved in the research pertaining to the Lignocellosic Conversion to Ethanol programme (LACE programme, funded by BBSRC Sustainable Bioenergy Centre). He developed a solid state fungal fermentation based pre-treatment strategy to convert lignocellulosic raw materials into a mixture of fermentable simple sugars. In this process, cellulolytic enzymes were produced by culturing fungus (Aspergillus niger) directly on the ...
0086]1. Aarestrup F. M. 1995. Occurrence of glycopeptide resistance among Enterococcus faecium isolates from conventional and ecological poultry farms. Microb Drug Resist. 1: 255-257. [0087]2. Abarca M. L, M. R. Bragulat, G. Castella and F. J. CabanesS. 1994. Ochratoxin A Production by Strains of Aspergillus niger var. niger. Applied and environmental Microbiology. 60: 2650-2652. [0088]3. Amann, R., Ludwig, W. & Schleifer, K. H. 1988. Subunit of ATP-synthase: a useful marker for studying the phylogenetic relationship of eubacteria. J Gen Microbiol. 134: 2815-2821. [0089]4. Ananthaswamy H. N and Eisenstark A (1977) Repair of hydrogen peroxide-induced single-strand breaks in Escherichia coli deoxyribonucleic acid. Journal of Bacteriology 130(1): 187-191. [0090]5. Angeles-Lopez M., E. G. Ramos E. G. C. and C. A. Santiago (2001) Hydrogen peroxide production and resistance to nonoxinol-9 in Lactobacillus spp. isolated from vagina of reproductive age women. Revista Latinoamericana de Microbiologia ...
Isopullulanase (IPU) from Aspergillus niger A.T.C.C. (American Type Culture Collection) 9642 hydrolyses pullulan to isopanose. IPU is important for the production of isopanose and is used in the structural analysis of oligosaccharides with α-1,4 and α-1,6 glucosidic linkages. We have isolated the ipuA gene encoding IPU from the filamentous fungi A. niger A.T.C.C. 9642. The ipuA gene encodes an open reading frame of 1695 bp (564 amino acids). IPU contained a signal sequence of 19 amino acids, and the molecular mass of the mature form was calculated to be 59 kDa. IPU has no amino-acid-sequence similarity with the other pullulan-hydrolysing enzymes, which are pullulanase, neopullulanase and glucoamylase. However, IPU showed a high amino-acid-sequence similarity with dextranases from Penicillium minioluteum (61%) and Arthrobacter sp. (56%). When the ipuA gene was expressed in Aspergillus oryzae, the expressed protein (recombinant IPU) had IPU activity and was immunologically reactive with ...
The catalytic domain of glucoamylases G1 and G2 from Aspergillus niger is produced in vitro in high yield by limited proteolysis using either subtilisin Novo or subtilisin Carlsberg. Purification by affinity chromatography on an acarbose-Sepharose column followed by ion-exchange chromatography on HiLoad Q-Sepharose leads to separation of a number of structurally closely related forms of domain. The cleavage occurs primarily between Val-470 and Ala-471 as indicated by C-terminal sequencing, whereas the N-terminus is intact. Subtilisin Carlsberg, in addition, produces a type of domain which is hydrolysed before Ser-444, an O-glycosylated residue. This leaves the fragment Ser-444-Val-470 disulphide-bonded to the large N-terminal part of the catalytic domain. Subtilisin Novo, in contrast, tends to yield a minor fraction of forms extending approx. 30-40 amino-acid residues beyond Val-470. The thermostability is essentially the same for the single-chain catalytic domain and the original glucoamylases ...
Abstract: The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers. ...
Aspergillus niger is a versatile filamentous fungus found in the environment all over the world in soil and on decaying plant material and it has been reported to grow on a large number of foods and feeds [1]. At the same time it is a popular production host for industrial fermentations and it is used for production of both organic acids and for indigenous and heterologous enzymes and proteins [2-4]. However, A. niger produces various secondary metabolites, and among those also the important mycotoxins fumonisin B2 (FB2) and ochratoxin A (OTA) [5, 6]. Due to the ubiquity of A. niger, its production of secondary metabolites is important both from a biotechnological and a food-safety viewpoint.. Secondary metabolites are small molecules that are not directly involved in metabolism and growth. Both plants and fungi are known for producing a large number of chemically diverse secondary metabolites. While the role of some of these metabolites makes sense biologically as inferring an advantage to the ...
BioAssay record AID 568480 submitted by ChEMBL: Antifungal activity against Aspergillus niger KCTC 1231 after 2 days by twofold broth dilution method.
BioAssay record AID 1085122 submitted by ChEMBL: Antifungal activity against Aspergillus niger at 100 ug/disk after 48 hr by disk diffusion test.
Spent refinery processing catalyst is listed as a hazardous waste; the toxicity characteristic leaching procedure (TCLP) extracts of the catalyst are found to contain heavy metals at concentrations exceeding the regulated levels. In the present investigation, Aspergillus niger was adapted to single metal ions Ni, Mo or Al (at 100-2000 mg/L in steps of 100 mg/L) and then to a mixture of Ni, Mo and Al (at a mass ratio of 1:2:6, as approximately present in the spent catalyst). Adaptation experiments with single metals showed that the fungus could tolerate up to 1000 mg/L Ni, 1200 mg/L Mo and 2000 mg/L Al. In the presence of a mixture of these metals, the fungus was able to tolerate up to 100 mg/L Ni, 200 mg/L Mo and 600 mg/L Al. One-step bioleaching experiments with 1 wt% spent catalyst (of particle size <37 μm) were carried out using un-adapted and various adapted fungal strains. In contrast to the adapted strains, the un-adapted strain showed no growth in the presence of the catalyst. ...
Continuous chemostat cultures of a recombinant strain of Aspergillus niger (B1-D), engineered to produce the marker protein hen egg white lysozyme, were investigated with regard to their susceptibility to oxidative stress. The culture response to oxidative stress, produced either by addition of exogenous hydrogen peroxide (H2O2) or by high dissolved oxygen tension (DOT), was characterised in terms of the activities of two key defensive enzymes: catalase (CAT) and superoxide dismutase (SOD). Since the morphology is so critical in submerged fungal bioprocesses, the key morphological indices were analysed using a semi-automated image analysis system. Both oxidant stressors, H2O2 and elevated DOT, increased both enzyme activities, however, the extent was different: exogenous H2O2 led mainly to increased CAT activity, whereas gassing with O2 enriched air, which resulted in a DOT of 165% of air saturation, increased both enzyme activities more than 2-fold compared with the control steady state ...
An acidophilic α-amylase from Aspergillus niger RBP7 was purified after solid state fermentation on potato peel substrate. Molecular mass of the purified α-amylase was 37.5 kDa and it exhibited 1.4 mg/ml and 0.992 μ/mol/min Km and Vmax values, respectively. The enzyme was stable in the pH range from 2.0 to 6.0, at high NaCl concentration (3 M) and at temperatures between 40 °C and 70 °C. The enzyme showed an optimal activity at pH 3.0 and at 45 °C. The enzyme was inhibited by Hg2+ and was stable in the presence of different surfactants (Tween 60, Tween 80, and SDS at 1% level) and different inhibitory reagents (β-mercaptoethanol, phenylmethylsulfonyl fluoride, and sodium azide). This acidophilic amylase enzyme can digest heterogeneous food materials, i.e. the mixture of rice, fish, bread and curry with comparable activity to the commercial diastase enzymes available.. ...
Purchase high purity enzyme endo-1-5-alpha-L-Arabinanase (Aspergillus niger) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.
Naringinase from Aspergillus niger was prepared and characterized to evaluate its effectiveness in debittering citrus juice. The enzyme was purified to homogeneity by sulfate fractionation and chromatographies on Q-Sepharose, Sephacryl S-200, and S-1
A NOVEL STRAIN OF Aspergillus niger PRODUCING A COCKTAIL OF HYDROLYTIC DEPOLYMERISING ENZYMES FOR THE PRODUCTION OF SECOND GENERATION BIOFUELS
We have started research to analyse the molecular mechanism underlying protein secretion in A. niger. In the first instance we have isolated 9 distinct GTPase encoding genes from A. niger (sarA, sagA-H) corresponding to GTPases involved in most stages of the secretory pathway. Interestingly, several of these genes homologues are present in higher eukaryotes, but not in S. cerevisiae. These genes are used to generate a set of (conditional/deletion) mutants imposing defined blocks in the secretory pathway. To analyse transport and secretion of proteins we have developed a GFP-based secretion reporter system by fusing GFP to a carrier protein, glucoamylase (GLA). Expression of a g1aA::gfp fusion construct resulted in fluorescence of the cell wall, probably representing secreted GLA::GFP fusion protein that is retained within the extracellular matrix. Periplasmic fluorescence was only observed in young mycelium. No periplasmic fluorescence is observed in older mycelia probably due to acidification ...
SBC) and E (7.5% untreated SBC). Total DMI rose (P , 0.05) with Aspergillus treated diets (B and D) than the untreated diets (C and E). The trend was similar for the rest of the variables (CPI and EEI) except for the crude fibre intake which did not show a specific trend. Dry matter digestibility for the control diet (A) was the lowest than any of the other diets while the highest dry matter digestibility was recorded ...
ID ASPNG_1_PE330 STANDARD; PRT; 155 AA. AC ASPNG_1_PE330; A2R3F4; DT 00-JAN-0000 (Rel. 1, Created) DT 00-JAN-0000 (Rel. 2, Last sequence update) DT 00-JAN-0000 (Rel. 3, Last annotation update) DE SubName: Full=Putative uncharacterized protein An14g04100;Flags: DE Precursor; (ASPNG_1.PE330). GN ORFNames=An14g04100; OS ASPERGILLUS NIGER. OC Eukaryota; Fungi; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiales; OC Trichocomaceae; mitosporic Trichocomaceae; Aspergillus. OX NCBI_TaxID=5061; RN [0] RP -.; RG -.; RL -.; CC -!- SEQ. DATA ORIGIN: Translated from the HOGENOM CDS ASPNG_1.PE330. CC Aspergillus niger chromosome I CADRE full sequence 1..3379275 annotated CC Ensembl Genomes CC -!- ANNOTATIONS ORIGIN:A2R3F4_ASPNC CC -!- GENE_FAMILY: HOG000175185 [ FAMILY / ALN / TREE ] DR HOGENOM:Aspergillus_niger;CADANGAG00011346;CADANGAT00011346;CADANGAP00011126. DR EMBL; AM270320; - ; DR UniProtKB/Swiss-Prot; A2R3F4; -. DR EMBL; AM270320; CAK46646.1; -; Genomic_DNA. DR RefSeq; XP_001401034.1; ...
Biotechnology Research International is a peer-reviewed, Open Access journal that publishes original research articles as well as review articles in all areas of biotechnology.
Endo-1,5-alpha-L-arabinanase involved in degradation of pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan (By similarity).
The [email protected] Centre provides a platform for research students to deposit their Ph.D. theses and make it available to the entire scholarly community in open access. Shodhganga Mirror Site ...
Beta-glucosidase is a glucosidase enzyme that acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). It is one o
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Common black mold found on plants. Produces abundant conidiophores. Medium: potato dextrose agar. Per plate.NOTE: Bacterial and fungal cultures will be sold and shipped to …
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Nai C, Meyer V. The beauty and the morbid: fungi as source of inspiration in contemporary art. Fungal Biology and Biotechnology 2016; 3:10 DOI: 10.1186/s40694-016-0028-4 Bignell E, Cairns T, Throckmorton K, Nierman W, Keller N. Secondary metabolite arsenal of an opportunistic pathogenic fungus. Phil. Trans. R. Soc. B; DOI: 10.1098/rstb.2016.0023 Paege N, Jung S, Schäpe P, Müller-Hagen D, Ouedraogo JP, Heiderich C, Jedamzick J, Nitsche BM, van den Hondel CA, Ram AF, Meyer V. A Transcriptome Meta-Analysis Proposes Novel Biological Roles for the Antifungal Protein AnAFP in Aspergillus niger. PlosOne 2016; DOI:10.1371/journal.pone.0165755.s009 Meyer V, Idnurm A. The freedom of choice. Fungal Biology and Biotechnology 2016; 3:9 DOI: 10.1186/s40694-016-0027-5 Meyer V, Andersen M, Brakhage A, Braus G, Caddick M, Cairns T, de Vries R, Haarmann T, Hansen K, Hertz-Fowler C, Krappmann S, Mortensen U, Peñalva M, Ram AF, Head R. Current challenges of research on filamentous fungi in relation to human ...
Allcosmeticsource.com Gluco-amylase 100000u/g,5kg/bag,free shipping [EP170508010]- Gluco-amylase 100000u/g,5kg/bag,free shipping What is Gluco-amylase 100000u/g Gluco-amylase is derived through extraction and refinement from the Aspergillus niger, with high enzyme activity and glucose conversion. Pullulanase is derived from Bacillus subtilis, which anhydrolyze liquefied starch of alpha -D-1,6 glucoside bounds to straight chain dextrinrapidly.Cooperation with high efficiency, it can convert the starch into glucose. Application of Gluco-amylase 100000u/g This product can be
this is vandna thakur . i want to construct a growth curve for Aspergillus niger .Can u help me to give some information about this .and can u tell me that can i construct a growth curve of A.niger by taking optical density ,if yes then tell me at which wavelenth ? Actually i want to construct a standard curve for A.niger.Reply meas soon as , plz. -------------- next part -------------- An HTML attachment was scrubbed... URL: http://www.bio.net/bionet/mm/yeast/attachments/20110320/2c5d6ff4/attachment.html ...
Comparison of pro-inflammatory cytokines (IL-6, IL-1α and IL-1β) released by MPI and MARCO (-/-) knockout cells when stimulated by heat killed fungi- Candida albicans and Aspergillus niger
Highly purified enzyme from Aspergillus niger used for isolation of biologically active protoplasts from a wide spectrum of higher plants and tissues.
A constitutive level of a mycelium-bound lipolytic activity from Aspergillus niger MYA 135 was strongly increased by 97% in medium supplemented with 2% olive oil. The constitutive lipase showed an optimal activity in the ...
Novus Scientific (www.novusscientific.com) är en innovatör inom forskning och kommersialisering av resorberbara implantat som hjälper kroppens egen läkning. Huvudkontor, utveckling och produktion ligger i Uppsala och försäljning sker via distributörer i ett växande antal länder, för närvarande ett tjugotal.Företagets ägare och företagsledning kommer från Uppsalabaserade Radi Medical Systems AB, köpt av St Jude Medical Inc 2008. Det först utvecklade och saluförda implantatet är TIGR® Matrix (www.TIGRmatrix.com) som är ett kirurgiska nät, som bidrar med mekanisk hållfasthet tills kroppen själv återskapat vävnadens styrka för att sedan utsöndras via naturliga vägar. Främsta användningsområden är: · Att stabilisera vävnaden runt implantat i samband med bröstrekonstruktion efter cancer. · Att återställa bukväggens funktion efter komplexa ärrbråck. · Att förstärka bukförslutningen efter öppenbukskirurgi för att minska risken för sårruptur och
2.1.1 Absorption, distribution, and excretion No available data. 2.2 Toxicological studies 2.2.1 Acute studies Species Route LD50 Reference (mg/kg/b.w.) Rat oral 5000 van Eeken et al., 1986a 2.2.2 Short-term studies 2.2.2.1 Rat Groups of 9-20 male and 10-20 female Wistar rats, 89-111 g b.w., were treated by gavage for 91 days with chymosin at daily dose levels of 0, 50, 5000 or 1000 mg/kg b.w. The report did not indicate whether the commercial enzyme preparation or a more concentrated or purified form of the enzyme was used in the study. No mortality was observed in experimental animals over the course of the study. Growth, behaviour and external appearance were normal. Haematology measurements in treated animals were comparable to controls. Statistically significant changes were occasionally observed in clinical chemistry parameters, but the changes were not dose-related. Absolute and relative organ weights were unaffected by treatment, except for liver weights in females, which exhibited a ...
Abstract: The effect of solid state fermentation of Aspergillus niger on millets nutritional quality and phytic acid content were investigated in this study. Ammonium sulphate and urea as Nitrogen sources were used in combination with millet at 10 gN kg 1 substrate. The mixture was fermented for 84 h at 35 C and 90-95% RH in the laboratory. The results indicated that the crude protein content of millet was raised from 10.96-17.33%, while the crude lipid was lowered fro 3.12-2.58%. The ash content value was greatly increased from 1.36-7.94%. Five percent changes in minerals (calcium, magnesium and phosphorus) content values were also observed. Phytic acid content value of 0.4 mg 100g 1 reduced significantly to minimal 0.015 mg 100g 1. ...
An existing way to produce glucose uses enzymes to hydrolyzepolysaccharides. Aspergillusniger is recognized to produce glucoamylases capable to reduce starch to...
A team of researchers is working to use naturally-occurring fungi for an environment-friendly recycling process to extract cobalt and lithium from waste batteries.. Get the Free Tracker App to find a SNES Classic in Stock. "The idea first came from a student who had experience extracting some metals from waste slag left over from smelting operations," said Jeffrey A. Cunningham, Ph.D. and the projects team leader.. Cunninghams team is developing the environmentally-safe way to do this with organisms found in nature -- fungi in this case -- and putting them in an environment where they can do their work.. "Fungi are a very cheap source of labor," he pointed out in his work to be presented at the 252nd National Meeting & Exposition of the American Chemical Society (ACS).. To drive the process, Cunningham and Valerie Harwood, Ph.D., both at the University of South Florida, are using three strains of fungi -- Aspergillus niger, Penicillium simplicissimum and Penicillium chrysogenum.. The team ...
Aspergillus niger endopolygalacturonase E: a cell degrading enzyme found in microorganisms and higher plants; nucleotide sequence in first source; GenBank Y14386
A gene of glucose oxidase (GOD) from Aspergillus niger Z-25 was cloned and sequenced. The entire open reading frame (ORF) consisted of 1,818 bp and encoded a putative peptide of 605 amino acids. The gene was fused to the pPICZαA plasmid and overexpressed in Pichia pastoris SMD1168. The recombinant GOD (rGOD) was secreted into the culture using MF-α factor signal peptide under the control of the AOX1 promoter. Sodium dodecyl sulfate polyacrylamide gel electrophoresis indicated that rGOD exhibited a single band at around 94 kDa. The maximal GOD activity of approximately 40 U/mL was achieved in shake flask by induction under optimal conditions after 7 days. rGOD was purified by ammonium sulfate precipitate leading to a final specific activity of 153.46 U/mg. The optimum temperature and pH of the purified enzyme were 40 °C and 6.0, respectively. Over 88% of maximum activity was maintained below 40 °C. And the recombinant enzyme displayed a favorable stability in the pH range from 4.0 to 8.0. The ...
4665-7604 recognises glucose oxidase from Aspergillus niger.This antibody has not been cross-adsorbed and may react with homologous proteins
glucose oxidase from aspergillus niger/ACM9001370 can be provided in Alfa Chemistry. We are dedicated to provide our customers the best products and services.
The secondary substrate binding site (SBS) of Bacillus subtilis and Aspergillus niger glycoside hydrolase family 11 xylanases was studied by site-directed mutagenesis and evaluation of activity and binding properties of mutant enzymes on different substrates. Modification of the SBS resulted in an up to three-fold decrease in the relative activity of the enzymes on polymeric versus oligomeric substrates and highlighted the importance of several amino acids in the SBS forming hydrogen bonds or hydrophobic stacking interactions with substrates. Weakening of the SBS increased Kd values by up to 70-fold in binding affinity tests using natural substrates. The impact that modifications in the SBS have both on activity and on binding affinity towards polymeric substrates clearly shows that such structural elements can increase the efficiency of these single domain enzymes on their natural substrates ...
Kinetics of Escherichia coli destruction by microwave irradiation. Tailing of thermal inactivation curve of Aspergillus niger spores
Good performance of p16/ki-67 dual-stained cytology for surveillance of women treated for high-grade CIN. Possible explanation of the basic human capacity for emotional experience Dephosphorylation of phytate by levitra vs viagra using the Aspergillus niger phytase with a high affinity for phytate. Identification of viagra substitute DNA-reactive B cells in patients with systemic lupus erythematosus. Contribution of exogenous genetic elements to the group A Streptococcus metagenome.. The FA-CS nanoparticles (NPs) were prepared by crosslinking FA-CS conjugates with sodium tripolyphosphate (STPP). The differentiation level could be sildenafil citrate estimated by two-dimensional cell morphology analysis using a conventional phase-contrast microscope. The observed preferences cannot be explained by any single structural principle, but point instead to the interaction of several principles. Catechols are co-planar cis-diols known to form female viagra pills stable, isolable complexes with borate ...