O-glycosylation has been considered a limiting factor in protein secretion in filamentous fungi. Overexpression of the yeast DPM1 gene encoding dolichylphosphate mannose synthase (DPMS) in an Aspergillus nidulans mutant (BWB26A) deficient in O-glycosylation caused an increase in the number of secretory vesicles and changes in protein secretion. However, the secretory proteins, primarily O-mannosylated glucoamylase and N-glycosylated invertase, were mainly trapped in the periplasmic space. Different glycoforms of invertase were found insite the cells, in the periplasmic space and in the cultivation medium. Our data point to the importance of the cell wall as a barrier in protein secretion ...
TY - JOUR. T1 - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation. AU - Nuetzmann, Hans-Wilhelm. AU - Reyes-Dominguez, Yazmid. AU - Scherlach, Kirstin. AU - Schroeckh, Volker. AU - Horn, Fabian. AU - Gacek, Agnieszka. AU - Schümann, Julia. AU - Hertweck, Christian. AU - Strauss, Joseph. AU - Brakhage, Axel A. PY - 2011/8/23. Y1 - 2011/8/23. N2 - Sequence analyses of fungal genomes have revealed that the potential of fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for the biosynthesis of antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it is one of the major challenges in microbiology to uncover the mechanisms required for pathway activation. Recently, we discovered that intimate physical interaction of the important model fungus Aspergillus nidulans with the soil-dwelling bacterium Streptomyces rapamycinicus specifically ...
The biosynthesis of the beta-lactam antibiotic penicillin in the filamentous fungus Aspergillus nidulans is catalyzed by three enzymes that are encoded by the acvA, ipnA, and aatA genes. A variety of cis-acting DNA elements and regulatory factors form a complex regulatory network controlling these beta-lactam biosynthesis genes. Regulators involved include the CCAAT-binding complex AnCF and AnBH1. AnBH1 acts as a repressor of the penicillin biosynthesis gene aatA. Until now, however, little information has been available on the signal transduction cascades leading to the transcription factors. Here we show that inhibition of protein kinase C (Pkc) activity in A. nidulans led to cytoplasmic localization of an AnBH1-enhanced green fluorescent protein (EGFP) fusion protein. Computer analysis of the genome and screening of an A. nidulans gene library revealed that the fungus possesses two putative Pkc-encoding genes, which we designated pkcA and pkcB. Only PkcA showed all the characteristic features ...
Hyphal tip growth in fungi is important because of the economic and medical importance of fungi, and because it may be a useful model for polarized growth in other organisms. We have investigated the central questions of the roles of cytoskeletal elements and of the precise sites of exocytosis and endocytosis at the growing hyphal tip by using the model fungus Aspergillus nidulans. Time-lapse imaging of fluorescent fusion proteins reveals a remarkably dynamic, but highly structured, tip growth apparatus. Live imaging of SYNA, a synaptobrevin homologue, and SECC, an exocyst component, reveals that vesicles accumulate in the Spitzenkörper (apical body) and fuse with the plasma membrane at the extreme apex of the hypha. SYNA is recycled from the plasma membrane by endocytosis at a collar of endocytic patches, 1-2 μm behind the apex of the hypha, that moves forward as the tip grows. Exocytosis and endocytosis are thus spatially coupled. Inhibitor studies, in combination with observations of ...
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). "Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae". Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
In this study, several aspects of nitrate assimilation and transport have been studied using the filamentous fungus Aspergillus nidulans, which has been shown to be safe laboratory organism as judged by its pathogenicity towards insect larvae. In silico analysis of the A. nidulans genome sequence, identified two putative genes designated cnxL and cnxK that might be involved in molybdenum cofactor (a component of nitrate reductase) biosynthesis as well as two putative nitrate reductases encoding genes niaB and niaC. All four genes are hitherto unknown. Although many features of these proteins provided clues of functionality, biochemical and genetical approaches employed in this present study failed to elicit expression of any of these four genes. A NrtA protein structure model was developed based on residue homology with the E. coli GlpT a protein, the structure of which has been solved. The results of thiol cross-linking of three double cysteine mutants in four NrtA essential residues, R87, ...
TY - JOUR. T1 - The Aspergillus nidulans snt genes are required for the regulation of septum formation and cell cycle checkpoints. AU - Kraus, Peter R.. AU - Harris, Steven D.. PY - 2001/11/17. Y1 - 2001/11/17. N2 - In Aspergillus nidulans, germinating conidia undergo multiple rounds of nuclear division before forming a septum. Previous genetic results suggest that the ability to separate nuclear division and septum formation depends upon a threshold level of activity of the cyclin-dependent kinase NIMX,cdk1. Mutations in nimX and nimT, the gene encoding the NIMXcdk1-activating phosphatase, have revealed that Tyr-15 phosphorylation is important for determining the timing of the formation of the first septum. Here, we describe a screen for suppressors of nimT23 (snt), designed to identify additional components of the pathway regulating septum formation. We show that a subset of the snt mutants are defective in the temporal regulation of septum formation and in cell cycle checkpoint responses. ...
The filamentous fungus Aspergillus nidulans senses red using the red light receptor, phytochrome (FphA). In this study, we identified the MAP kinase SakA (also known as HogA) was involved in phytochrome-dependent light sensing by an efficient screening approach. FphA physically interacted with the histidine-containing phosphotransferase (HPt), YpdA in the cytoplasm. Light activation of the SakA pathway depended on FphA. Hence, light plugs into the SakA pathway through phytochrome ...
TY - JOUR. T1 - Analysis of ambient pH stress response mediated by iron and copper intake in Schizosaccharomyces pombe. AU - Higuchi, Yujiro. AU - Mori, Hikari. AU - Kubota, Takeo. AU - Takegawa, Kaoru. PY - 2018/1/1. Y1 - 2018/1/1. N2 - The molecular mechanism of tolerance to alkaline pH is well studied in model fungi Aspergillus nidulans and Saccharomyces cerevisiae. However, how fission yeast Schizosaccharomyces pombe survives under alkaline stress remains largely unknown, as the genes involved in the alkaline stress response pathways of A. nidulans and S. cerevisiae were not found in the genome of this organism. Since uptake of iron and copper into cells is important for alkaline tolerance in S. cerevisiae, here we examined whether iron and copper uptake processes were involved in conferring tolerance to alkaline stress in S. pombe. We first revealed that S. pombe wild-type strain could not grow at a pH higher than 6.7. We further found that the growths of mutants harboring disruption in the ...
Aspergilli represent an extremely important genus of microorganisms which can be both harmful pathogens, and beneficial pharmaceutical producers. In Aspergillis interactions with man, suboptimal nutrient conditions are often present, and lead to a phenomenon known as autophagy. Autophagy is a cellular recycling mechanism that (in the case of macroautophagy) is augmented under nutrient limited conditions to recycle cytoplasmic macromolecules and organelles for use in essential cell functions. Strategic manipulation of autophagy could ultimately lead to improved bioprocesses or anti-fungal treatments. Using the model filamentous fungus Aspergillus nidulans, a number of important questions about autophagy have been addressed.. Critical to the study of autophagy is the balance between self-degradation and self-preservation. Therefore, we adapted an XTT metabolic activity assay for use in filamentous fungi. The assay was first tested using a number of bioprocess related stresses (e.g. temperature, ...
Conidiation in the filamentous ascomycete Aspergillus nidulans requires activation of brlA, a well-characterized transcriptional regulator of genes that are induced specifically during asexual develop
Entry into mitosis in Aspergillus nidulans is regulated by the coordinate function of two serine/threonine protein kinases, NIMXCDC2 and NIMA. NIMXCDC2 is an essential histone H1 kinase that is structurally and functionally homologous to fission yeast p34cdc2 (Osmani et al., 1994). NIMA is a β-casein kinase and is structurally distinct from p34cdc2, containing an amino-terminal catalytic domain and a carboxyl-terminal regulatory domain (Osmani et al., 1988b; Lu et al., 1993; Pu and Osmani, 1995; Pu et al., 1995). Failure to properly activate either of these kinases in G2 prevents the initiation of mitosis, and the combined action of both kinases is critical for coordinating changes in chromosome, microtubule, and nuclear membrane structure during mitosis. For example, mutations preventing the activation of NIMXCDC2 in G2 normally arrests cells in late G2 (Osmani et al., 1991a; 1994). Although overexpression of NIMA can overcome this interphase arrest, the ensuing mitosis is disorganized such ...
Summary: A mutation in a gene designated gmdA has been found to lead to loss of ability of Aspergillus nidulans to use benzamide, phenylacetamide and several other amides as sole nitrogen sources for growth. The gmdAI lesion results in low levels of an enzyme, called the general amidase, which has activity for a wide range of amide substrates. This enzyme is repressed by certain nitrogen-containing metabolites, including ammonium, but is probably not regulated by induction or by carbon catabolite repression. Evidence is presented for the general amidase being distinct from the previously characterized acetamidase and formamidase enzymes. The data also indicate that there is a fourth amidase capable of the hydrolysis of valeramide and hexanamide.
TamA interacts with LeuB, the homologue of Saccharomyces cerevisiae Leu3p, to regulate gdhA expression in Aspergillus nidulans Journal Articles Refereed ...
Molecular cloning and expression in Saccharomyces cerevisiae of two Aspergillus nidulans xylanase genes.: Two Aspergillus nidulans genes, xlnA and xlnB, encodin
a haploid strain of the aspergillus nidulans carried the auxotrophic met-eight mutation conferring a requirement for, Hire Biology Expert, Ask Academics Expert, Assignment Help, Homework Help, Textbooks Solutions
PLEASE HELP We are the parents of a nineteen month old Baby Boy with a life threatening disease. To our knowledge this is the first case of a fungal infection of this type known anywhere in the world. If you can provide any suggestions for helpign our little boy we would be most grateful. Infection: Aspergillus Nidulans in the Central Nervous System. The fungus surrounds the base of the brain and is present in other locations on the covering of the meninges. This was diagnosed following a biopsy taken from his lumbar region. Biopsy was taken September 13, 1996. Cause of Infection: Unknown Patients Present Condition: Beginning to show signs of Hydrocephalus. Vomiting is becoming more frequent and fevers and pain are becoming more frequent and severe. He is developing a little trouble walking. Course of Treatment: Began treatment in September on Amphotericin B and 5FC given by IV. Treatment was determined to be unsuccessful. After one month MRI showed disease had progressed. The next treatment ...
Aspergillus nidulans ATCC ® 24766™ Designation: FGSC A158 (ribo1 bi1) Application: Biomedical Research and Development Material
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It has recently been proposed that acetaldehyde is the physiological inducer of the alc gene system and hence indirectly the activator of the AlcA promoter in Aspergillus nidulans. Here we show that t...
Microbial steroid biotransformation have found wide-reaching application for the production of more precious and functionalized compounds due to their high regio-and stereoselectivity. In this study, the possibility of using filamentous fungi Aspergillus brasiliensis cells in the biotransformation of progesterone (I), a C-21 steroid hormone was studied for the first time.The fungal strain was inoculated into the transformation medium which supplemented with progesterone as a substrate. Biotransformation of this steroid for 7 days afforded 3 different hydroxylated metabolites: 11α-hydroxyprogesterone (II); 14α-hydroxyprogesterone (III) and 21-hydroxyprogesterone (IV).The metabolites were separated by thin layer chromatography. Structure determinations of the metabolites were performed by comparing NMR, MS and IR spectra of starting compound with those of metabolites.These results may be of industrial importance because the metabolites can be used as precursor of some steroid drugs.
In this paper, we characterized the Dis1/XMAP215 family protein AlpA from A. nidulans and found that it is associated with the MT plus end during mitosis and in interphase. AlpA plays a role in controlling MT dynamics and is important for the determination of growth polarity. Whereas the mechanism of MT stabilization was recently shown in S. cerevisiae (1), a role in polarized growth has not been described before. Polarized growth of filamentous fungi depends on the continuous delivery of secretory vesicles (7, 20). These vesicles provide new membranes and deliver, e.g., enzymes for cell wall biosynthesis. Because the vesicles are generated some distance away from the growing tip, they need to be transported long distances. It is assumed that MTs and conventional kinesin provide the basis for this long-distance transportation (18, 23). The first destination of the vesicles is an organelle close to the apex named the vesicle supply center or Spitzenkörper (8). The location of this organelle ...
Dear David, Ive formulated the concept of a gene module, a set of genes used by more than one step of differentiation, in: Gordon, R. (1995). The Hierarchical Genome and Differentiation Waves: Novel Unification of Development, Genetics, and Evolution (Singapore: World Scientific), in prep. Might this match your clusters? To what extent are you dealing with housekeeping genes? To what extent are they related via gene duplication? Best regards, -Dick Gordon, U.Manitoba[May19,95] On 18 May 1995, David H. Griffin wrote: , Is anyone looking at the evolution of genome organization, specifically , clustering of genes with related functions. This has been reported , frequently among fungi, but these clusters are not always universal, , for example the proline genes of Aspergillus nidulans are clustered but , those of Saccharomyces cerevisiae are not. Is gene clustering of , physiologically related genes common amongst other organisms than , fungi? Any examples with references, or at least ...
THE filamentous fungi form mycelial colonies that consist of networks of branched hyphae that grow by apical extension. In the higher fungi (i.e., Ascomycota and Basidiomycota), hyphae are compartmentalized by the formation of cross-walls, or septa. It has long been suspected that the presence of septa allows filamentous fungi to partition cellular environments within a hypha to support colony homeostasis and reproductive development (Gull 1978). The process of septum formation is similar to cytokinesis of animal cells, in that it coordinated with mitosis and requires formation of a contractile actin ring (CAR) (Balasubramanian et al. 2004). By analogy to the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, the CAR likely provides a landmark that guides deposition of the septal wall material. However, unlike these yeasts, the septum is not subsequently degraded and cells remain attached. Furthermore, in most filamentous fungi, a small pore is retained to enable communication ...
per liter NaNO3 6.0 gm KC1 0.52 gm MgSO4. 7H20 0.52 gm KH2PO4 1.52 gm Adjust pH to ca 6.5 (usually requires 1 ml of 1 N NaOH) (Note: If large quantities of minimal are to be used a 2OX stock solution of the above salts can be prepared.) glucose (dextrose) 10.0 gms 2 ml of Hutners trace elements For agar add 15.0 gms Difco Agar ...
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An endo-1,5-arabinanase (abnA) encoding gene from Aspergillus niveus was identified, cloned and successfully expressed in Aspergillus nidulans strain A773. Based on amino acid sequence comparison, the 34-kDa enzyme could be assigned to CAZy GH family 43. Characterization of purified recombinant endo-1,5-arabinanase (AbnA) revealed that it is active at a wide pH range (pH 4.0-7.0) and an optimum temperature at 70 °C. The immobilization of the AbnA was performed via covalent binding onto agarose-modified supports: glyoxyl iminodiacetic acid-Ni 2+, glyoxyl amine, glyoxyl (4% and 10%) and cyanogen bromide activated sepharose. The yield of immobilization was similar on glyoxyl amine and glyoxyl (96%), and higher than glyoxyl iminodiacetic acid-Ni 2+ (43%) support. The thermal inactivation of these immobilized preparations showed that the stability of the AbnA immobilized on glyoxyl 4 and 10% was improved by 4.0 and 10.3-fold factor at 70 °C. The half-life of glyoxyl 4% derivative at 60 °C was ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Older research outputs will score higher simply because theyve had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 260,964 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 61% of its contemporaries ...
Transcription factor that mediates regulation of both acid- and alkaline-expressed genes in response to ambient pH. At alkaline ambient pH, activates transcription of alkaline-expressed genes (including RIM101 itself), mainly by repressing transcriptional repressors of those genes, and represses transcription of acid-expressed genes. Required for meiosis, sporulation and invasive growth.
We have shown that A. nidulans can distinguish between conditions of nitrogen sufficiency, nitrogen limitation, and nitrogen starvation and adjust accordingly the expression levels of nitrogen catabolic genes. These changes are AreA dependent and reflect alterations in the level and activity of AreA. Furthermore, we have shown that A. nidulans can respond to nitrogen starvation by altering the dynamics of nuclear entry and exit of AreA, using regulated nuclear exit to provide a rapid response when nutritional conditions change.. We favor a model in which, in the presence of nitrogen sources, AreA is present in the nucleus at a relatively low level due to a balance between nuclear entry and exit. When repressing metabolites are at high levels (for example, when ammonium or glutamine is the source of nitrogen), the level of transcriptionally active AreA is low due to accelerated areA mRNA turnover and NmrA inhibition of AreA activation ability (1, 32). When poorer (limiting) nitrogen sources are ...
veA+ is used to indicate the wild-type allele. Please refer to Han, et al (2010) Simple identification of veA1 mutation in Aspergillus nidulans. J Microbiol. 2010 Dec;48(6):885-7.. ...
BACKGROUND: Fungi are important players in the turnover of plant biomass because they produce a broad range of degradative enzymes. Aspergillus nidulans, a well-studied saprophyte and close homologue to industrially important species such as A. niger and A. oryzae, was selected for this study. RESULTS: A. nidulans was grown on sorghum stover under solid-state culture conditions for 1, 2, 3, 5, 7 a ...
Galagan JE et al. Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature 438 (7071), 1105-1115 (2005 ...
The enzyme contains heme. The bifunctional enzyme from Aspergillus nidulans uses different heme domains to catalyse two separate reactions. Linoleic acid is oxidized with
Pontecorvo, G., Roper, J.A., Chemmons, L.M., Macdonald, K.D. and Bufton, A.W.J. (1953) The genetics of Aspergillus nidulans. Advances in Genetics, 5, pp. 141-238. (doi:10.1016/S0065-2660(08)60408-3) ...
Specimen Code: CUP-LP-000261; Determined By: G.T. Hill; Date Determined: Sep 13,1999; Slant: no; -80° C Freezer: no; Herbarium: CCFB; d H2O: n/a; Collected By: G.T. Hill; Date(s) Collected: 13 Sep 1999; Soil type: BoE; Source: on rotten wood; Locality Code: Sector 22; Locality Name: Lindsay-Parsons Biodiversity Preserve; Township: West Danby, Tompkins Co.; State: NY; Country: USA; Elevation: 900-1400 ft.; Loc Latitude: 42°1836N; Loc Longitude: 76°3036W ...
Urrence No Yes PKCa overexpression Negative Positive 1 0.677 0.444?.032 0.0699 1 3.494 2.117?.766 ,0.0001 1 5.224 3.435?.944 ,0.0001 1 6.147 3.627?0.420 ,0.0001
Bicikl ima geometrijo in opremo, ki se imo svetuje rekreativcu začetniku, ki ima malce resnejši namen pogostega odkrivanja kolovozov in stezic za hišo. Nima pa še izkušenj, časa in/ali konjske kondicije. Oprema je kvalitativno zelo uravnotežena. Razred deore je primeren tudi za hujšo zlorabo in je cenovno ugoden (menjava komponent zaradi obrabe) rekreativcu, ki tedensko naredi do 100km (2000 višincev), nima izrazite tehnike vožnje, ter še išče svoje mesto v različnih oblikah gorskega kolesarjenja (in nima manije vsak gram šteje:). Pogosto se menja gonilni ležaj, račna, verižnika in veriga,... Če opisane menjaš 1x letno te v razredu material deore pride okrog 80€. V razredu deore XT najmanj 160€, v razredu XTR pa te 170€-200€ pride samo zadnji verižnik ...
Dai unocchiata a questi esempi di libri sfogliabili per bambini per trovare ispirazione in vista delle tue pubblicazioni future e dei progetti da te ideati.
Coloured scanning electron micrograph (SEM) of Aspergillus nidulans hyphae and sexual spores. A. nidulans (also known as Emericella nidulans) is a filamentous fungus that is the only member of the genus Aspergillus that forms sexual spores through meiosis (seen here in this image as large spores). A. nidulans is also a homothallic fungus, meaning it can form asexual spores by producing conidiphores that bear chains of asexual spores. A. nidulans. has been an important research organism for studying eukaryotic cell biology and has a long history of use for the study of a wide range of subjects including basic genetic problems (recombination, DNA repair, mutation), cell cycle control and pathogenesis, as well as metabolism of other Aspergillus species. A. nidulans produces the toxin, sterigmatocysin, which may lead to food contamination. Magnification: x440 when shortest axis printed at 25 millimetres. - Stock Image C032/3077
Coloured scanning electron micrograph (SEM) of Aspergillus nidulans hyphae and sexual spores. A. nidulans (also known as Emericella nidulans) is a filamentous fungus that is the only member of the genus Aspergillus that forms sexual spores through meiosis (seen here in this image as large spores). A. nidulans is also a homothallic fungus, meaning it can form asexual spores by producing conidiphores that bear chains of asexual spores. A. nidulans. has been an important research organism for studying eukaryotic cell biology and has a long history of use for the study of a wide range of subjects including basic genetic problems (recombination, DNA repair, mutation), cell cycle control and pathogenesis, as well as metabolism of other Aspergillus species. produces the toxin, sterigmatocysin, which may lead to food contamination. Magnification: x440 when shortest axis printed at 25 millimetres. - Stock Image C032/3079
TY - JOUR. T1 - The Aspergillus nidulans sepA gene encodes an FH1/2 protein involved in cytokinesis and the maintenance of cellular polarity. AU - Harris, Steven D.. AU - Hamer, Lisbeth. AU - Sharpless, Kathryn E.. AU - Hamer, John E.. PY - 1997/6/16. Y1 - 1997/6/16. N2 - Cytokinesis (septation) in the fungus Aspergillus nidulans occurs through the formation of a transient actin ring at the incipient division site. Temperature-sensitive mutations in the sepA gene prevent septation and cause defects in the maintenance of cellular polarity, without affecting growth and nuclear division. The sepA gene encodes a member of the growing family of FH1/2 proteins, which appear to have roles in morphogenesis and cytokinesis in organisms such as yeast and Drosophila. Results from temperature shift and immunofluorescence microscopy experiments strongly suggest that sepA function requires a preceding mitosis and that sepA acts prior to actin ring formation. Deletion mutants of sepA exhibit ...
Yu, J.H. and T.J. Leonard. 1998. Culture conditions control expression of the genes for alfatoxin and sterigmatocystin biosynthesis in Aspergillus parasiticus and Aspergillus nidulans. Appl. Environ. Microbiol. 64: 2275-2277.. Yu, J.H., R. Butchko, M. Fernandes, N. Keller, T.J. Leonard, T.H. Adams. 1996. Conservation of structure and function of the aflatoxin regulatory gene aflR from Aspergillus nidulans and A. flavus. Current Genetics 29:549-555.. Brown, D.W., J.H. Yu, H.S. Kelkar, M. Fernandes, T.C. Nesbit, T.C. Keller, N.P. Adams and T.J. Leonard. 1996. Twenty-five co-regulated transcripts define a secondary metabolism gene cluster in Asperfillus nidulans. Proc. Natl. Acad. Sci. USA. 93:1418-1422.. Feng, G.H. and T.J. Leonard. 1995. Characterization of the polyketide synthase gene (pksLi) required for aflatoxin biosynthesis in Aspergillus nidulans. J. Bact. 177: 6246-6254.. Yu, J.H. and T.J. Leonard. 1995. Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I ...
Purine hydroxylase I from Aspergillus nidulans was purified 850-fold. The purified preparations exhibited the spectral and catalytic properties, including broad specificity for oxidizing and reducing substrates, typical of molybdenum/flavin/iron-sulphur-containing hydroxylases (oxotransferases).
We present the case of a 3-year-old boy who was diagnosed with cerebral abscesses due to Aspergillus nidulans infection on day 28 of induction chemotherapy for acute lymphoblastic leukemia. He responded well to treatment with voriconazole and caspofungin, making a full recovery. There are very few cases of invasive aspergillosis reported in children during induction chemotherapy for acute leukemia and A. nidulans is rare in the absence of chronic granulomatous disease.
The catabolism of glucose and xylose was studied in a wild type and creA deleted (carbon catabolite de-repressed) strain of Aspergillus nidulans. Both strains were cultivated in bioreactors with either glucose or xylose as the sole carbon source, or in the presence of both sugars. In the cultivations on single carbon sources, it was demonstrated that xylose acted as a carbon catabolite repressor (xylose cultivations), while the enzymes in the xylose utilisation pathway were also subject to repression in the presence of glucose (glucose cultivations). In the wild type strain growing on the sugar mixture, glucose repression of xylose utilisation was observed; with xylose utilisation occurring only after glucose was depleted. This phenomenon was not seen in the creA deleted strain, where glucose and xylose were catabolised simultaneously. Measurement of key metabolites and the activities of key enzymes in the xylose utilisation pathway revealed that xylose metabolism was occurring in the creA ...
Filamentous fungi play vital roles in human health, agriculture and bioprocessing. In all of these situations fungi are often exposed to nutrient limitation, which can impact their behavior. Autophagy is a eukaryotic cellular process, induced under nutrient deficient conditions, which recycles internal components for cell survival. Autophagy can also be gratuitously induced in rich growth medium, using the drug rapamycin. To address our hypotheses, we have studied fungi and cell wall material properties of the model fungus, Aspergillus nidulans, under autophagic conditions. For example, it has been observed that during fungal fermentation nutrient limitation leads to a greater degree of hyphal fragmentation. We hypothesize that under nutrient deprived conditions autophagy is involved in recycling cell wall components, thereby weakening the wall and resulting in this increased degree of fragmentation. In all experiments, fungi were subjected to two media conditions - with and without rapamycin. ...