Aspergillus tubingensis XghA protein Summary Report | CureHunter
Aspergillus tubingensis XghA protein: pectinolytic enzyme from Aspergillus tubingensis; amino acid sequence in first source; GenBank AJ249460
Marine Drugs | Free Full-Text | Lumazine Peptides from the Marine-Derived Fungus Aspergillus terreus
Terrelumamides A (1) and B (2), two new lumazine-containing peptides, were isolated from the culture broth of the marine-derived fungus Aspergillus terreus. From the results of combined spectroscopic and chemical analyses, the structures of these compounds were determined to be linear assemblies of 1-methyllumazine-6-carboxylic acid, an amino acid residue and anthranilic acid methyl ester connected by peptide bonds. These new compounds exhibited pharmacological activity by improving insulin sensitivity, which was evaluated in an adipogenesis model using human bone marrow mesenchymal stem cells. In addition, the compounds exhibited fluorescence changes upon binding to DNA, demonstrating their potential applications to DNA sequence recognition.
Indian Patents. 250388:A HYBRID ENZYME FOR STARCH PROCESSING
Preferred for the invention is CBMs of Carbohydrate-Binding Module Family 20. CBMs of Carbohydrate-Binding Module Family 20 suitable for the invention may be derived from glucoamylases of Aspergillus awamori (SWISSPROT Q12537), Aspergillus kawachii (SWISSPROT P23176), Aspergillus niger (SWISSPROT P04064), Aspergillus oryzae (SWISSPROT P36914), from alpha-amylases of Aspergillus kawachii (EMBL#AB008370), Aspergillus nidulans (NCBI AAF17100.1), from beta-amylases of Bacillus cereus (SWISSPROT P36924), or from CGTases of Bacillus circulans (SWISSPROT P43379). Preferred is a CBM from the alpha-amyiase of Aspergillus kawachii (EMBL:#AB008370) as well as CBMs having at least 50%, 60%, 70%, 80% or even at least 90% homology to the CBM of the alpha-amyiase of Aspergillus kawachii (EMBL#AB008370), i.e. a CBM having at least 50%, 60%, 70%, 80% or even at least 90% homology to the amino acid sequence of SEQ ID NO:6. Also preferred for the invention are the CBMs of Carbohydrate-Binding Module Family 20 ...
Sequence determination of a quadripartite dsRNA virus isolated from Aspergillus foetidus
Virus infection of Aspergillus foetidus was documented over 40 years ago and was one of the first mycovirus infections described in a filamentous fungus. The virus, named Aspergillus foetidus virus (AfV), contains at least two types of icosahedral particles, called AfV-fast (-F) and AfV-slow (-S) virions, based on their relative electrophoretic mobilities. Here, we report the complete nucleotide sequence of the AfV-F genome isolated from virions purified from the prototype isolate of the fungus. The AfV-F double-stranded (ds) RNA genome is tetra-segmented, and the plus strands of each of the four segments, but not the minus strands, are polyadenylated. The organisation and sequences of the four AfV-F dsRNAs are similar to those described for Alternaria alternata virus 1, which we propose is a member of an emerging mycovirus genus (Alternavirus) and family (Alternaviridae), which also includes AfV-F ...
Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus awamori var. kawachi)
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href=http://www.uniprot.org/manual/proteomes_manual>proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
Forests | Free Full-Text | Aspergillus tubingensis Is a Pre-Emergent Pathogen of Date Palm Seedlings
Many diseases of date palm are known. However, pathogens that might affect seed germination and seedling emergence from soil are poorly studied, perhaps because date palm cultivars are propagated vegetatively. Here, we first determined the effects of date seed fungi on the germination and emergence of 600 seeds overall (i.e., 200 of each of three cultivars: ‘Thoory’, ‘Halawi’, and ‘Barhi’). In each cultivar, 100 seeds were from Saudi Arabia (part of the native range), and 100 were from the southwestern USA (where the date palm was introduced around 1765). Just four fungal genera (i.e., Alternaria, Aspergillus, Chaetomium, and Penicillium) were isolated from the surface-sterilized date seeds. Aspergillus isolates all belonged to Aspergillus sect. Nigri; collectively they were in the highest relative abundance at 39%, and significantly more common in Saudi Arabian seeds than in American seeds. Aspergillus reduced seed germination and also reduced emergence when
Aspergillus clavatus | Mycology | Online Microbiology Notes
Habitat, Morphology, Cultural Characteristics, Life Cycle, Pathogenesis, Lab Diagnosis, Treatment and Prevention of Aspergillus clavatus.
Kinetic Modelling of Lovastatin Biosynthesis by Aspergillus Terreus Cultivated on Lactose and Glycerol as Carbon Sources -...
A kinetic model to describe lovastatin biosynthesis by Aspergillus terreus ATCC 20542 in a batch culture with the simultaneous use of lactose and glycerol as carbon sources was developed. In order to do this the kinetics of the process was first studied. Then, the model consisting of five ordinary differential equations to balance lactose, glycerol, organic nitrogen, lovastatin and biomass was proposed. A set of batch experiments with a varying lactose to glycerol ratio was used to finally establish the form of this model and find its parameters. The parameters were either directly determined from the experimental data (maximum biomass specific growth rate, yield coefficients) or identified with the use of the optimisation software. In the next step the model was verified with the use of the independent sets of data obtained from the bioreactor cultivations. In the end the parameters of the model were thoroughly discussed with regard to their biological sense. The fit of the model to the ...
Aspergillus terreus Thom ATCC ® 20542D-2™
Aspergillus terreus ATCC ® 20542D-2™ Designation: Genomic DNA from Aspergillus terreus strain MF4845 (ATCC ® 20542™) Application:
Aspergillus terreus Thom ATCC ® 20542D-2™
Aspergillus terreus ATCC ® 20542D-2™ Designation: Genomic DNA from Aspergillus terreus strain MF4845 (ATCC ® 20542™) Application:
Aspergillus sp. YT-008 - Aspergillus and Aspergillosis
Reference (original paper): Benzaldehyde derivatives from a marine-derived fungus Aspergillus sp. Huang, Yuling; Ma, Liying; Rong, Xianguo; Liu, Desheng; Liu, Shaojun; Liu, Weizhong. Zhongcaoyao (2012), 43(5), 837-840. Currently accepted name (anamorph): Aspergillus sp. YT-008. Computed content type (Species): Species. ...
New resources for functional analysis of omics data for the genus Aspergillus | BMC Genomics | Full Text
A detailed and comprehensive genome annotation can be considered a prerequisite for the analysis and interpretation of omics data. GO provides a framework for functional annotation and has been proven to be a valuable tool for omics data analysis, especially in combination with enrichment statistics. Currently, the GO reference genome project [23] provides the most comprehensive manually curated GO annotation for twelve model organisms and is intended to serve as a reference for automated mapping of GO annotation to organisms other than these major models. From the reference genome projects, Saccharomyces cerevisiae and Schizosaccharomyces pombe are most closely related to the genus Aspergillus.. A. nidulans has so far been the only Aspergillus species with comprehensive genome scale GO annotation based on both orthology mapping to S. cerevisiae and extensive manual curation [9] of gene-specific literature. We have thus mapped the A. nidulans GO annotation to all other Aspergillus species (see ...
Most recent papers with the keyword Aspergillus fumigatus | Read by QxMD
Non-fumigatus Aspergillus species are the leading cause of Aspergillus infections in the tropical and subtropical regions of the world. In a prospective study between 2015 and 2016, a total of 150 bronchoalveolar (BAL) specimens was collected from patients suspected to pulmonary aspergillosis (PA) underlying immunodeficiencies in Mashhad, Northeastern Iran, located in the Middle East. All Aspergillus strains were phylogenetically identified at the species level by PCR-sequencing of partial β-tubulin gene. Overall, Aspergillus species were isolated from 20 specimens originating from 10 (50%) patients with cancer, 5 (25%) patients receiving corticosteroid therapy, 3 (15%) organ transplant recipients and 2 (10%) patients admitted to intensive care unit (ICU ...
Aspergillus giganteus | Semantic Scholar
A strain of Aspergillus giganteus cultivated in a medium with xylan produced two xylanases (xylanase I and II) which were… Expand ...
List of Aspergillus species - Wikipedia
List of fungus species in the genus Aspergillus. The Aspergillus genus includes several hundred fungus species, including: Contents A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Aspergillus acidusAspergillus aculeatinusAspergillus aculeatus Aspergillus aeneusAspergillus affinisAspergillus alabamensisAspergillus alliaceus Aspergillus amazonicusAspergillus ambiguusAspergillus amoenusAspergillus amstelodamiAspergillus amyloliquefaciensAspergillus amylovorusAspergillus anomalusAspergillus anthodesmisAspergillus apicalisAspergillus appendiculatusAspergillus arachidicolaAspergillus arenariusAspergillus arviiAspergillus asperescensAspergillus assulatusAspergillus astellatusAspergillus aurantiobrunneusAspergillus aureofulgensAspergillus aureolatusAspergillus aureoterreusAspergillus aureusAspergillus auricomusAspergillus australensisAspergillus austroafricanusAspergillus avenaceusAspergillus awamori Top A B C D E F G H I J K L M N O P Q R S T U V W X Y Z Aspergillus baarnensisAspergillus ...
Phylogeny, identification and nomenclature of the genus Aspergillus - Danish National Research Database-Den Danske...
Aspergillus comprises a diverse group of species based on morphological, physiological and phylogenetic characters, which significantly impact biotechnology, food production, indoor environments and human health. Aspergillus was traditionally associated with nine teleomorph genera, but phylogenetic data suggest that together with genera such as Polypaecilum, Phialosimplex, Dichotomomyces and Cristaspora, Aspergillus forms a monophyletic clade closely related to Penicillium. Changes in the International Code of Nomenclature for algae, fungi and plants resulted in the move to one name per species, meaning that a decision had to be made whether to keep Aspergillus as one big genus or to split it into several smaller genera. The International Commission of Penicillium and Aspergillus decided to keep Aspergillus instead of using smaller genera. In this paper, we present the arguments for this decision. We introduce new combinations for accepted species presently lacking an Aspergillus name and ...
Orizin - Wikipedia
Orizin (EC 3.4.21.63, Aspergillus alkaline proteinaza, aspergilopeptidaza B, API 21, aspergilopepsin B, aspergilopepsin F, Aspergillus candidus alkalna proteinaza, Aspergillus flavus alkalna proteinaza, Aspergillus melleus semialkalna proteinaza, Aspergillus oryzae alkalna proteinaza, Aspergillus parasiticus alkalna proteinaza, Aspergillus serin proteinaza, Aspergillus sydowi alkalna proteinaza, Aspergillus soya alkalna proteinaza, Aspergillus melleus alkalna proteinaza, Aspergillus sulphureus alkalna proteinaza, prozim, P 5380, kiorinaza, seaproza S, semi-alkalna proteaza, sumizim MP, prozim 10, onoproza, onoproza SA, proteaza P, promelaza) je enzim.[1][2][3][4][5] Ovaj enzim katalizuje sledeću hemijsku reakciju. ...
Purification and biochemical characterization of feruloyl esterases from Aspergillus terreus MTCC 11096
Aspergillus terreus MTCC 11096 isolated from the soils of agricultural fields cultivating sweet sorghum was previously identified to produce feruloyl esterases (FAEs). The enzymes responsible for feruloyl esterase activity were purified to homogeneity and named as AtFAE-1, AtFAE-2, and AtFAE-3. The …
JCVI: What Can Comparative Genomics Tell Us About Species Concepts In the Genus Aspergillus?
Citation. Rokas, A., Payne, G., Fedorova, N. D., Baker, S. E., Machida, M., Yu, J., Georgianna, D. R., Dean, R. A., Bhatnagar, D., Cleveland, T. E., Wortman, J. R., Maiti, R., Joardar, V., Amedeo, P., Denning, D. W., Nierman, W. C.. What Can Comparative Genomics Tell Us About Species Concepts In the Genus Aspergillus?. Stud Mycol. 2007 Jan 01; 59: 11-7.. PubMed Citation. Abstract. Understanding the nature of species boundaries is a fundamental question in evolutionary biology. The availability of genomes from several species of the genus Aspergillus allows us for the first time to examine the demarcation of fungal species at the whole-genome level. Here, we examine four case studies, two of which involve intraspecific comparisons, whereas the other two deal with interspecific genomic comparisons between closely related species. These four comparisons reveal significant variation in the nature of species boundaries across Aspergillus. For example, comparisons between A. fumigatus and Neosartorya ...
DIGITAL.CSIC: Immobilisation of fructosyltransferase from Aspergillus aculeatus on epoxy-activated Sepabeads EC for the...
The transfructosylating activity present in two commercial pectinase preparations (Pectinex Ultra SP-L, from Aspergillus aculeatus, and Rapidase TF, from Aspergillus niger) was studied. Pectinex Ultra SP-L, which has a high transferase/hydrolase ratio, was covalently immobilised on a polymethacrylate-based polymer (Sepabeads® EC) activated with epoxy groups. The influence of pore volume and average pore size on biocatalyst performance was studied for two of these carriers (Sepabeads EC-EP3 and EC-EP5). Several parameters that affect immobilisation such as buffer concentration, pH and amount (mg) of protein added per gram of support (varied over the range 30:1 to 200:1) were analysed. We found that Pectinex Ultra SP-L can be efficiently immobilised on these supports without adding any external salt or buffer. Using Sepabeads EC-EP5 -whose pore volume (1.67 cm3/g) and pore size (800 nm) are higher than those corresponding to Sepabeads EC-EP3- the activity towards sucrose reached 25.9 U/g ...
Neoxaline - Wikipedia
Neoxaline is a bio-active Aspergillus japonicus isolate. It is an antimitotic agent and shows weak inhibitory activity of blood platelet aggregation induced by simulation of the central nervous system. It has been synthesized through the highly stereoselective introduction of a reverse prenyl group to create a quaternary carbon stereocenter using (−)-3a-hydroxyfuroindoline as a building block, construction of the indoline spiroaminal via cautious stepwise oxidations with cyclizations from the indoline, assembly of (Z)-dehydrohistidine, and photoisomerization of unnatural (Z)-neoxaline to the natural (E)-neoxaline. Satoshi Ōmura Neoxaline an antimiotic agent Hirano, A; Iwai, Y; Masuma, R; Tei, K; Omura, S (1979). Neoxaline, a new alkaloid produced by Aspergillus japonicus. Production, isolation and properties. J Antibiot (Tokyo). 32 (8): 781-785. doi:10.7164/antibiotics.32.781. PMID 500498. Ideguchi, Tetsuya; Yamada, Takeshi (August 11, 2013). Asymmetric Total Synthesis of Neoxaline. The ...
Studies of Food Thickeners in Nigeria for Contamination by Aflatoxigen by G. I. Okwu, Premila Achar et al.
This paper reports the contamination of ready-to-use food thickeners, collected from the South-East geo-political zone in Nigeria, by aflatoxigenic form of Aspergillus species. A total of 150 samples from different open markets were observed for fungal contamination by using serial dilution-spread plate method. Although, Aspergillus, Fusarium and Penicillium were the most frequently isolated fungi, Aspergillus species were found to be the most prevalent in all the samples. Furthermore, Aspergillus flavus and Aspergillus parasiticus produced aflatoxin on yeast extract sucrose (YES) media incubated for 10 to 15 days at 27°C in a CO2 incubator. Aspergillus niger showed no sign of any secondary metabolite on the media, set at similar conditions. Although, light microscopy was used to identify these fungi, based on colony morphology, PCR method was used to confirm genetic variation among the Aspergillus group, using ITS set
Profiling differential expression of cellulases and metabolite footprints in Aspergillus terreus. - Semantic Scholar
This study reports differential expression of endoglucanase (EG) and beta-glucosidase (betaG) isoforms of Aspergillus terreus. Expression of multiple isoforms was observed, in presence of different carbon sources and culture conditions, by activity staining of poly acrylamide gel electrophoresis gels. Maximal expression of four EG isoforms was observed in presence of rice straw (28 U/g DW substrate) and corn cobs (1.147 U/ml) under solid substrate and shake flask culture, respectively. Furthermore, the sequential induction of EG isoforms was found to be associated with the presence of distinct metabolites (monosaccharides/oligosaccharides) i.e., xylose (X), G(1), G(3) and G(4) as well as putative positional isomers (G(1)/G(2), G(2)/G(3)) in the culture extracts sampled at different time intervals, indicating specific role of these metabolites in the sequential expression of multiple EGs. Addition of fructose and cellobiose to corn cobs containing medium during shake flask culture resulted in up
Effect of foliar spraying of Aspergillus terreus Thom on sheath blight (ShB) and rice plant characteristics
Gogoi, R.; Roy, A.K., 1993: Effect of foliar spraying of Aspergillus terreus Thom on sheath blight (ShB) and rice plant characteristics
Chronic Aspergillus sp. meningitis successfully treated with fluconazole: case report
The patient stayed 12 days at the hospital, and was submitted to several radiological and laboratory tests. Computerized axial tomography and resonance magnetic imaging were normal. The enzyme-linked immunosorbent assay (ELISA) for HIV 1 and 2 was repeatedly negative. Other two samples of CSF were collected and cultures to bacteria and fungi were negative, slide latex agglutination (SLA) tests to cryptococcal antigen, Haemophilus influenza, Neisseria meningitidis, Streptococcus pneumoniae were negative. The patient was followed on consultations and five CSF examinations were done during a follow up period of a month, when the diagnosis was finally established. In the first CSF sample, a polymerase chain reaction (PCR) to Mycobacterium tuberculosis was negative. In the next CSF sample, culture to fungi was positive to Aspergillus sp and the immunodiffusion test showed presence of antibodies against Aspergillus sp. Other immunological tests to Histoplasma, Cysticercus and Sporothrix were negative. ...
KEGG PATHWAY: Glycolysis / Gluconeogenesis - Aspergillus clavatus
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
ACLA 013420 gene cDNA ORF clone, Aspergillus clavatus NRRL 1 - GenScript
Next-day shipping cDNA ORF clones derived from ACLA_013420 Cytochrome P450 oxidoreductase, putative available at GenScript, starting from $99.00.
DSpace at KOASAS: Cytochrome P450 Monooxygenase-Mediated Metabolic Utilization of Benzo[a]Pyrene by Aspergillus Species
Soil-dwelling fungal species possess the versatile metabolic capability to degrade complex organic compounds that are toxic to humans, yet the mechanisms they employ remain largely unknown. Benzo[a]pyrene (BaP) is a pervasive carcinogenic contaminant, posing a significant concern for human health. Here, we report that several Aspergillus species are capable of degrading BaP. Exposing Aspergillus nidulans cells to BaP results in transcriptomic and metabolic changes associated with cellular growth and energy generation, implying that the fungus utilizes BaP as a growth substrate. Importantly, we identify and characterize the conserved bapA gene encoding a cytochrome P450 monooxygenase that is necessary for the metabolic utilization of BaP in Aspergillus. We further demonstrate that the fungal NF-kappa B-type velvet regulators VeA and VelB are required for proper expression of bapA in response to nutrient limitation and BaP degradation in A. nidulans. Our study illuminates fundamental knowledge of ...
Aspergillus species - The Cardiology Advisor
Balajee, SA, Marr, KA. Phenotypic and genotypic identification of human pathogenic aspergilli. Future Microbiol. vol. 1. 2006. pp. 435-45. Camps, SM, Rijs, AJ, Klaassen, CH. Molecular epidemiology of isolates harboring the TR34/L98H azole resistance mechanism. J Clin Microbiol. vol. 50. 2012. pp. 2674-80. Cornely, OA, Maertens, J, Bresnik, M. Liposomal amphotericin B as initial therapy for invasive mold infection: a randomized trial comparing a high-loading dose regimen with standard dosing (AmBiLoad trial). Clin Infect Dis. vol. 44. 2007. pp. 1289-97. Cornely, OA, Maertens, J, Winston, DJ. Posaconazole vs fluconazole or itraconazole prophylaxis in patients with neutropenia. N Eng J Med. vol. 356. 2007. pp. 348-59. DHaese, J, Theunissen, K, Vermeulen, E. Detection of galactomannan in bronchoalveolar lavage fluid samples of patients at risk for invasive pulmonary aspergillosis: analytical and clinical validity. J Clin Microbiol. vol. 50. 2012. pp. 1258-63. Herbrecht, R, Denning, DW, ...
Asterriquinone (2) | Aspergillus & Aspergillosis Website
Metabolic products of Aspergillus terreus. VI. Metabolites of the strain IFO 8835. (3). The isolation and chemical structures of colorless metabolites. Arai, Kunizo; Shimizu, Sakae; Yamamoto, Yuzuru. Chemical & Pharmaceutical Bulletin (1981), 29(4), 1005-12 Metabolic products of Aspergillus terreus. X. Biosynthesis of asterriquinones. Arai, Kunizo; Yamamoto, Yuzuru. Chemical & Pharmaceutical Bulletin (1990), 38(11), 2929-32 ...
Abstract: Detection of Aspergillus Spp. in Human Tissues Samples by Immunohistochemistry and in Situ Hybridization (48th Annual...
Background: The diagnosis of invasive asperigllosis (IA) is limited by poor sensitivity and specificity of microbiologic cultures, and the inability of conventional histopathologic tests to distinguish IA from invasive fungal infections (IFI) caused by non-Aspergillus moulds. Aspergillus-specific immunohistochemistry (IHC) and in situ hybridization (ISH) could facilitate direct diagnosis of IA within tissue. Methods: IHC and ISH were performed on deparaffinized, formalin-fixed sections from previously diagnosed cases of proven IFI, and slides interpreted by pathologist blinded to diagnosis. IHC: Primary Aspergillus antibody (Abcam, MA) and secondary anti-rabbit antibody were detected with Avidin Biotin Complex and visualized using liquid DAB. ISH: Commercially synthesized Locked Nucleic Acid probe for Aspergillus was detected using antiflourescein AP. Poly T stained tissue section was used to determine the area of tissue to be studied. Results: 8 tissue sections were available from patients with ...
Hydrophobins from Aspergillus species cannot be clearly divided into two classes - DTU Orbit
TY - JOUR. T1 - Hydrophobins from Aspergillus species cannot be clearly divided into two classes. AU - Jensen,Britt Guillaume. AU - Andersen,Mikael Rørdam. AU - Pedersen,Mona Højgaard. AU - Frisvad,Jens Christian. AU - Søndergaard,Ib. PY - 2010. Y1 - 2010. N2 - Background Hydrophobins are a family of small secreted proteins with a characteristic pattern of eight cysteine residues found exclusively in filamentous fungi. They have originally been divided into two classes based on their physical properties and hydropathy patterns, and are involved in the attachment of hyphae to hydrophobic structures, the formation of aerial structures and appear to be involved in pathogenicity. Findings Analysis of nine genome sequences from seven Aspergilli revealed fifty hydrophobins, where each species displayed between two to eight hydrophobins. Twenty of the identified hydrophobins have not previously been described from these species. Apart from the cysteines, very little amino acid sequence homology was ...
Profilaxis de la infección por Aspergillus con anfotericina B nebulizada en pacientes transplantados pulmonares - Dipòsit...
Esta tesis se compone de dos articulos: 1. Profilaxis con anfotericina B nebulizada contra la infección por Aspergillus en el trasplante de pulmón: Estudio de factores de riesgo. J Heart Lung Transplant 2001;20: 1274-1281. Introducción: La infección por Aspergillus spp. permanece como una causa importante de morbilidad y mortalidad en el trasplante de pulmón (TP). Algunas estrategias de profilaxis han sido ensayadas. Una de ellas es la anfotericina B nebulizada (nAB). Sin embargo, la eficacia de esta profilaxis no ha sido claramente demostradas. El objetivo de este estudio fue determinar si la profilaxis con nAB puede proteger contra la infección por Aspergillus spp. en los receptores de TP. Pacientes y métodos: Un estudio de factores de riesgo fue realizado en 55 pacientes con TP. 22 potenciales factores de riesgo fueron analizados. En 44 (80%) de los pacientes la nAB fue indicada como profilaxis. Se realizó un estudio multivariante utilizando regresión logística. Resultados: Dieciocho de 55
Aspergillus - EAPCRI
Despite the fact that PCR for the detection of Aspergillus in human specimens exists for almost two decades, the technique was not included in the European Organisation for Research and Treatment of Cancer and the Mycoses Study Group (EORTC/MSG) consensus definitions for diagnosing invasive fungal diseases (IFD) because of the lack of standardisation. In 2006, the UK Fungal PCR Consensus group published an agreed methodology for PCR aided diagnosis of IA within the UK and Ireland. Then, at the meeting of the International Society for Human and Animal Mycoses (ISHAM) in Paris in September 2006, a group of mainly European experts founded the European Aspergillus PCR Initiative (EAPCRI) involving more than 60 centres across Europe and centres in Australia and the Middle East.. The EAPCRI agreed to collaborate to develop a standard for Aspergillus PCR methodology and to validate this in clinical trials so that PCR could be incorporated into future consensus definitions for diagnosing IFD.. The ...
Evaluation of Aspergillus PCR Protocols for Testing Serum Specimens
A panel of human serum samples spiked with various amounts of Aspergillus fumigatus genomic DNA was distributed to 23 centers within the European Aspergillus PCR Initiative to determine analytical performance of PCR. Information regarding specific methodological components and PCR performance was requested. The information provided was made anonymous, and meta-regression analysis was performed to determine any procedural factors that significantly altered PCR performance. Ninety-seven percent of protocols were able to detect a threshold of 10 genomes/ml on at least one occasion, with 83% of protocols reproducibly detecting this concentration. Sensitivity and specificity were 86.1% and 93.6%, respectively. Positive associations between sensitivity and the use of larger sample volumes, an internal control PCR, and PCR targeting the internal transcribed spacer (ITS) region were shown. Negative associations between sensitivity and the use of larger elution volumes (>/=100 mul) and PCR targeting ...
Definition of lovastatin - NCI Drug Dictionary - National Cancer Institute
A lactone metabolite isolated from the fungus Aspergillus terreus with cholesterol-lowering and potential antineoplastic activities. Lovastatin is hydrolyzed to the active beta-hydroxyacid form, which competitively inhibits 3-hydroxyl-3-methylgutarylcoenzyme A (HMG-CoA) reductase, an enzyme involved in cholesterol biosynthesis.
KIT - Other Aspergillus groups
Axel Brakhage. Director of the Leibniz-Institute for Natural Product Research and Infection Biology - Hans-Knoell-Institute (HKI). and. Professor and Chair of Microbiology / Molecular Biology. Friedrich-Schiller-University Jena. Department Molecular and Applied Microbiology. Research. Pathogenicity of Aspergillus fumigatus and secondary metabolism of Aspergillus nidulans. ...
Diversity and specificity of microsatellites within Aspergillus section Fumigati - Open Access Library
Background Microsatellites (or short tandem repeats, STRs) are the genetic markers of choice for studying Aspergillus fumigatus molecular epidemiology due to its reproducibility and high discrimination power. However, the specificity of these markers must ...
Misdiagnosis of Malt Workers disease -- Aspergillus spp. - RightDiagnosis.com
Misdiagnosis of Malt Workers disease - Aspergillus spp. including hidden diseases, diagnosis mistakes, alternative diagnoses, differential diagnoses, and misdiagnosis.
Aspergillus spp. | Johns Hopkins HIV Guide
Aspergillus spp. answers are found in the Johns Hopkins HIV Guide powered by Unbound Medicine. Available for iPhone, iPad, Android, and Web.
Aspergillus parasiticus
The University of Minnesota is one of the most comprehensive public universities in the United States and ranks among the most prestigious. It is both the state land-grant university, with a strong tradition of education and public service, and the states primary research university, with faculty of national and international reputation.
Identification of Common Aspergillus Species
Finden Sie alle Bücher von Klich, Maren A. - Identification of Common Aspergillus Species. Bei der Büchersuchmaschine eurobuch.com können Sie antiquarische und Neubücher VERGLEICHEN UND SOFORT zum Bestpreis bestellen. 9789070351465
SEM Aspergillus mould producing spores (conidia) | Europeana
Scanning electron micrograph of |I|Aspergillus|/I| producing spores, computer-coloured green. The picture shows an |I|Aspergillus|/I| conidiophore with conidia (spores) budding off (tissues stained blue). |I|Aspergillus|/I| is a genus of ascomycetes, some species of which cause aspergillosus or severe asthmatic reactions in humans; other species have industrial uses, e.g. in the processing of soybeans and the fermentation of sake.
Myconosticas MycAssay™ Aspergillus Test to Improve Diagnosis of Aspergillus Bronchitis in Patients with Cystic Fibrosis
Dr Caroline Baxter reported highly sensitive performance of MycAssay™ Aspergillus in CF sputum which is 260% more sensitive than the currently used culture.
ATEG 04263 - Metallophos domain-containing protein - Aspergillus terreus (strain NIH 2624 / FGSC A1156) - ATEG 04263 gene &...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Azole-Resistance in Aspergillus terreus and Related Species: An Emerging Problem or a Rare Phenomenon? - Kölner...
Die Universität zu Köln ist eine Exzellenzuniversität mit dem klassischen Fächerspektrum einer Volluniversität. Als eine der größen Hochschulen Europas arbeitet sie in Forschung und Lehre auch international auf höchstem Niveau.
Aspergillus terreus
The University of Minnesota is one of the most comprehensive public universities in the United States and ranks among the most prestigious. It is both the state land-grant university, with a strong tradition of education and public service, and the states primary research university, with faculty of national and international reputation.
Aspergillus clavatus | definition of Aspergillus clavatus by Medical dictionary
Looking for online definition of Aspergillus clavatus in the Medical Dictionary? Aspergillus clavatus explanation free. What is Aspergillus clavatus? Meaning of Aspergillus clavatus medical term. What does Aspergillus clavatus mean?
Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran<...
TY - JOUR. T1 - Species assignment and antifungal susceptibilities of black aspergilli recovered from otomycosis cases in Iran. AU - Szigeti, Gyöngyi. AU - Sedaghati, Ebrahim. AU - Mahmoudabadi, Ali Zarei. AU - Naseri, Ali. AU - Kocsubé, S.. AU - Vágvölgyi, C.. AU - Varga, J.. PY - 2012/7. Y1 - 2012/7. N2 - Black aspergilli are among the main causative agents of otomycosis worldwide. In this study, the species assignment of black aspergilli isolated from otomycosis cases in Iran was carried out using sequence analysis of part of the calmodulin gene. The results indicate that Aspergillus niger is not the only black Aspergillus species involved in otomycosis cases in Iran: Aspergillus awamori and Aspergillus tubingensis are also able to cause ear infections. Antifungal susceptibility tests were carried out against five antifungal drugs including amphotericin B, fluconazole, itraconazole, ketoconazole and terbinafine. All isolates were highly susceptible to terbinafine, while they exhibited ...
Aspergillus galactomannan antigen in the cerebrospinal fluid of bone marrow transplant recipients with probable cerebral...
TY - JOUR. T1 - Aspergillus galactomannan antigen in the cerebrospinal fluid of bone marrow transplant recipients with probable cerebral aspergillosis. AU - Viscoli, Claudio. AU - Machetti, Marco. AU - Gazzola, Paola. AU - De Maria, Andrea. AU - Paola, Dimitri. AU - Van Lint, Maria Teresa. AU - Gualandi, Francesca. AU - Truini, Mauro. AU - Bacigalupo, Andrea. PY - 2002. Y1 - 2002. N2 - The Aspergillus galactomannan test was performed on cerebrospinal fluid and serum samples from 5 patients with probable cerebral aspergillosis and from 16 control patients. Cerebrospinal fluid galactomannan levels were significantly higher in aspergillosis patients, and most galactomannan was produced intrathecally. Comparison of serum galactomannan values in pulmonary and cerebral aspergillosis patients showed significant overlapping. Detection of Aspergillus galactomannan in cerebrospinal fluid may be diagnostic of cerebral aspergillosis.. AB - The Aspergillus galactomannan test was performed on cerebrospinal ...
Fungal endophthalmitis caused by Aspergillus ustus in a patient following cataract surgery<...
TY - JOUR. T1 - Fungal endophthalmitis caused by Aspergillus ustus in a patient following cataract surgery. AU - Taner Yildiran, Sinasi. AU - Mehmet Mutlu, Fatih. AU - Ali Saracli, Mehmet. AU - Uysal, Yusuf. AU - Gonlum, Ahmet. AU - Sobaci, Gungor. AU - Sutton, Deanna A.. PY - 2006/11. Y1 - 2006/11. N2 - The first case of postoperative endophthalmitis after cataract surgery caused by Aspergillus ustus, a species that has only rarely been implicated in human disease, is described. Six weeks after cataract surgery, a 67-year-old medically controlled diabetic patient presented with uveitis, mild ciliary injection and ocular discomfort. Anterior chamber paracentesis, vitreous tap and finally complete vitrectomy with removal of the capsular bag including the intraocular lens were performed and several sets of culture yielded A. ustus. Despite vigorous systemic (itraconazole and caspofungin) and intravitreal (amphotericin B and caspofungin) antifungal therapy, the endophthalmitis did not improve. The ...
LOINC 51529-6 - Mold Allergen Mix 4 (Aspergillus fumigatus+Aspergillus niger+Aspergillus terreus+Aspergillus flavus) IgE Ab ...
LOINC Code 51529-6 Mold Allergen Mix 4 (Aspergillus fumigatus+Aspergillus niger+Aspergillus terreus+Aspergillus flavus) IgE Ab [Presence] in Serum by Multidisk
Repositório Institucional da UnB: Estudos do potencial do fungo Aspergillus tamarii na degradação da biomassa lignocelulósica
Nesta tese foram feitos estudos mais aprofundados de enzimas produzidas pelo fungo Aspergillus tamarii em diferentes contextos de degradação de biomassa lignocelulósica. O primeiro trabalho avaliou a influência de condições de cultivo na ação de celulases e xilanases e demonstrou que ferramentas estatísticas (CCD e CCRD) são necessárias para avaliar o comportamento da enzima em diferentes condições. Os resultados demonstraram que utilização de triptona como fonte de nitrogênio inibiu fortemente a atividade das celulases, enquanto aumentou das xilanases. Além disso, a suplementação com CuSO4 aumentou a atividade de todas as enzimas. Sugere-se que influência dos componentens do meio de culturam devem ser considerados ao fazer um planjamento de coquetel enzimático de fungo. O segundo trabalho avaliou os parâmetros termodinâmicos de uma xilanase pura de baixa massa molecular (22 kDa) de A. tamarii ativa em ácido ferúlico. Após análise de molecular docking de uma xilanase ...
Evaluation of orange peel, an industrial waste, for the production of Aspergillus sojae polygalacturonase considering both...
Orange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by Aspergillus sojae using statistical approaches. Effect of pH, another significant parameter affecting the rheology and morphology of the strain, was investigated in the serial bioreactor system using the optimized medium composition. Highest PG enzyme yield and productivity together with the maximum PG enzyme ...
Partial purification and characterization of phytase from Aspergillus foetidus MTCC 11682 | AMB Express | Full Text
Phytase is a phosphatase enzyme widely used as feed additive to release inorganic phosphorus from plant phytate and enhance its uptake in monogastric animals. Although engineered fungal phytases are used most, a natural enzyme gives opportunity to understand novel properties, if any. In the current study, a novel fungal strain, Aspergillus foetidus MTCC 11682 was immobilized on poly urethane cubes and used for phytase production, purification and molecular characterization. Phytase produced by this method was partially purified by ammonium sulphate precipitation and Sephacryl S-200HR gel filtration to 23.4-fold (compared to crude extract) with recovery of 13% protein. Electrophoresis analysis revealed that phytase has molecular weight of 90.5 kDa on non-reducing and 129.6 kDa on reducing SDS-PAGE. The purified phytase exhibited a wider pH and temperature stability. Analysis of the cloned sequence showed that the gene has 1176 bp that encodes for a peptide of 391 amino acids of the core catalytic region.
Genome-wide identification of the fatty acid desaturases gene family in four Aspergillus species and their expression profile...
Fatty acid desaturases play a key role in producing polyunsaturated fatty acids by converting single bonds to double bonds. In the present study, a total of 13, 12, 8 and 8 candidate fatty acid desaturases genes were identified in the Aspergillus oryzae, Aspergillus flavus, Aspergillus fumigatus and Aspergillus nidulans genomes through database searches, which were classified into five different subfamilies based on phylogenetic analysis. Furthermore, a comprehensive analysis was performed to characterize conserved motifs and gene structures, which could provide an intuitive comprehension to learn the relationship between structure and functions of the fatty acid desaturases genes in different Aspergillus species. In addition, the expression pattern of 13 fatty acid desaturases genes of A. oryzae was tested in different growth stages and under salt stress treatment. The results revealed that the fatty acid desaturases genes in A. oryzae were highly expressed in adaptive phase growth and up-regulated
Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases | Biotechnology for Biofuels |...
Declining oil reserves, political instability, climate change concerns and rising CO2 emissions have led to new interest in biofuels to supplement the growing demand for alternative and sustainable sources of transport fuels. Biofuels, which includes bio-ethanol, can be produced from renewable biomass resources that include dedicated crops (e.g. corn), by-products from agricultural processing activities (e.g. sugarcane bagasse) or even organic municipal waste. Although the positive environmental impact and sustainable nature of biofuels render it advantageous over fossil fuels [1], the cost-effective production of biofuels remains a challenge.. Starch, one of the most abundant polysaccharides in nature, has been used for commercial bio-ethanol production for a number of years, with a relatively mature technology developed for corn in the USA [2, 3]. The USA produced 52.6 billion litres of ethanol fuel in 2011, an increase from 49.2 billion litres in 2010 [4]. However, the limitations of current ...
Green mould conidiophore (Aspergillus flavus), SEM - Stock Image C037/0238 - Science Photo Library
Scanning electron micrograph (SEM) of Green mould (Aspergillus flavus) - conidiophore (fruiting structure) producing conidia (asexual conidia). Aspergillus flavus is a wide spread saprophyte found in soil, seeds, fruits and decaying vegetation. It is also found indoors on water damaged carpets and building materials. It is one of the Aspergillus species which causes aspergillosis, commonly in the tropics. Aspergillosis is a respiratory lung infection in people with weakened lungs or that are immune deficient (especially AIDS patients). This fungus produces aflatoxin, a group of virulent mycotoxins that are known animal carcinogens. These mycotoxins are can induce liver cancer in humans. The toxins may contaminate stored food (particularly carbohydrates). A. flavus has also been reported to be allergenic and its presence is associated with asthma. Magnification: x220 when shortest axis printed at 25 millimetres. - Stock Image C037/0238
Preparation of Protoplasts - Plant Growth Media - Culture Media - Cell Biology - Life Sciences
Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Pectolyase Y-23 is a highly purified maceration enzyme from Aspergillus japonicus. It contains two types of pectinase such as endopolygalacturonase and endo-pectin lyase in high activity. Included is a maceration stimulating factor which stimulates tissue maceration by both pectinases. Unit Definition: One unit is defined as an increase in A235 by 1.0 in the reaction mixture per minute.. ...
ASPBA - Performance: Aspergillus Antigen, Bronchoalveolar Lavage
ASPBA : The Platelia Aspergillus enzyme immunoassay (EIA) is a 1-stage immunoenzymatic sandwich microplate assay that detects galactomannan in bronchoalveolar lavage (BAL) specimens. The assay uses the rat monoclonal antibody EBA-2, which is directed against Aspergillus galactomannan. The monoclonal antibody is used 1) to coat the wells of the microplate and bind the antigen and 2) as the detector antibody in the conjugate reagent (peroxidase-linked monoclonal antibody). Samples are heat-treated in the presence of EDTA in order to dissociate immune complexes and to precipitate proteins that could possibly interfere with the test. The treated samples and conjugate are added to the wells coated with the monoclonal antibody and incubated. A monoclonal antibody-galactomannan-monoclonal antibody/peroxidase complex is formed in the presence of Aspergillus antigen. The strips are washed to remove any unbound material, and the substrate solution is added, which will react with the
OSA | Changes of delayed luminescence spectra in rice of different polluted degree by Aspergillus flavus
Light-induced delayed luminescence (DL) of living organisms contains information on the quality of the living state of these organisms. Employing a LS-55 Luminescence Spectrometer, the changes of DL spectra of rice with Aspergillus flavus treating different time were observed. Rice with Aspergillus flavus treatment for a shorter time had a stronger intensity of DL. The polluted degree of Aspergillus showed a negative correlation with the intensity of DL. Comparing with the intensity of DL, we found that the concentration of aflatoxin in different polluted degree rice had negative correlation with the intensity of DL. We believe DL technique may be helpful in elaborating a fast, holistic, and non-invasive method for the rapid evaluation the polluted degree of rice by Aspergillus flavus.. © 2005 Chinese Optics Letters. PDF Article ...
Novel Aspergillus hemicellulases enhance performance of commercial cellulases in lignocellulose hydrolysis - Shin - 2011 -...
A novel hemicellulase-producing fungal strain was isolated from a local soil sample. The organism is identified as Aspergillus fumigatus based on ribosomal RNA analyses. The Aspergillus strain, designated as 2NB, produces both enzymes acting on xylan backbone (xylanase and β-xylosidase), and those acting on side chains (or accessory enzymes) notably α-arabinofuranosidase and acetyl-xylan esterase. The Asperigillus hemicellulases are characterized as having relatively low xylanase and β-xylosidase activities but high side chain removal activities. The activity ratio of side-chain acting enzymes to xylanase is higher than that of the Multifect enzyme, a commercial hemicellulase product. The potential of the novel hemicellulases in lignocelluloses bioprocessing was demonstrated with alkaline-pretreated switchgrass as lignocellulose substrate with hemicellulase supplemented with a ratio of xylanase activity to filter paper unit of 2:1. Supplement of Aspergillus hemicellulases to commercial ...
Phosphatidylinositol transfer protein sfh5(sfh5) | Technique alternative | 01022755170 - Phosphatidylinositol
Order Aspergillus clavatus Phosphatidylinositol transfer protein sfh5 sfh5 -Mammalian Cell 01022755170 at Gentaur Aspergillus clavatus Phosphatidylinositol transfer protein sfh5 (sfh5) Mammalian
Difference between revisions of Aspergillus nidulans - microbewiki
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
Difference between revisions of Aspergillus nidulans - microbewiki
1 Adams, T. H., Wieser, J. K., & Yu, J.-H. (1998). Asexual Sporulation in Aspergillus nidulans. Microbiology and Molecular Biology Reviews, 62(2), 545-545. doi: 10.1128/mmbr.62.2.545-545.1998 https://mmbr.asm.org/content/62/1/35.full 2 David, H., Özçelik, I. Ş., Hofmann, G., & Nielsen, J. (2008). Analysis of Aspergillus nidulans metabolism at the genome-scale. BMC Genomics, 9(1), 163. doi: 10.1186/1471-2164-9-163 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2386489/ 3 Galagan JE; et al. (2005). Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae. Nature. 438 (7071): 1105-15. doi:10.1038/nature04341. PMID 16372000. https://www.ncbi.nlm.nih.gov/pubmed/16372000 4 Gugnani, H. C. (2003). Ecology and taxonomy of pathogenic aspergilli. Frontiers in Bioscience, 8(6). doi: 10.2741/1002 ...
UniProt: G7XC55 ASPKW
ID G7XC55_ASPKW Unreviewed; 497 AA. AC G7XC55; DT 25-JAN-2012, integrated into UniProtKB/TrEMBL. DT 25-JAN-2012, sequence version 1. DT 22-NOV-2017, entry version 26. DE SubName: Full=Aspartyl aminopeptidase {ECO:0000313,EMBL:GAA84418.1}; GN ORFNames=AKAW_02533 {ECO:0000313,EMBL:GAA84418.1}; OS Aspergillus kawachii (strain NBRC 4308) (White koji mold) (Aspergillus OS awamori var. kawachi). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus. OX NCBI_TaxID=1033177 {ECO:0000313,EMBL:GAA84418.1, ECO:0000313,Proteomes:UP000006812}; RN [1] {ECO:0000313,EMBL:GAA84418.1, ECO:0000313,Proteomes:UP000006812} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=NBRC 4308 {ECO:0000313,Proteomes:UP000006812}; RX PubMed=22045919; DOI=10.1128/EC.05224-11; RA Futagami T., Mori K., Yamashita A., Wada S., Kajiwara Y., RA Takashita H., Omori T., Takegawa K., Tashiro K., Kuhara S., Goto M.; RT Genome sequence of the white ...
Safety and Efficacy Study of Inhaled AmBisome for Prevention of Aspergillus Colonization in Lung Transplant Recipients -...
Lung transplant recipients have the highest rate of Invasive Aspergillus (IA)infection among solid organ transplant recipients. The most important risk factor for the development of IA (which is associated with disease and death) is colonization of the organism in the respiratory tract.. Azoles are used to prevent the development of IA. Puffers containing antifungal medication can be used to treat the lungs without the need to worry about the medication interactions & side-effects in the blood. An example of this is the aerosolized amphotericin B. Its use is limited by the patients tolerating this medication that may cause cough, nausea & contraction of the air pathways.. The lipid preparation is better tolerated and has longer dosing interval than inhaled amphotericin B. The investigators propose a pilot study to determine the long-term safety of inhaled AmBisome administration of drug and generate the preliminary data on the effectiveness of this drug to prevent aspergillus colonization. ...
Frontiers | Essential APSES Transcription Factors for Mycotoxin Synthesis, Fungal Development, and Pathogenicity in Aspergillus...
Aflatoxins are a potent carcinogenic mycotoxin and has become a research model of fungal secondary metabolism (SM). Via systematically investigating the APSES transcription factors (TFs), two APSES proteins were identified: AfRafA and AfStuA. These play central roles in the synthesis of mycotoxins including aflatoxin and cyclopiazonic acid, and fungal development and are consequently central to the pathogenicity of the aflatoxigenic A. flavus. Loss of AfRafA not only dramatically suppressed aflatoxin cluster expression, subsequently reducing toxin synthesis both in vitro and in vivo, but also impaired conidia and sclerotia development. More importantly, aflatoxin biosynthesis as well as conidia and sclerotia development were fully blocked in ΔAfStuA. In addition, our results supported that AfStuA regulated the aflatoxin synthesis in an AflR-dependent manner. Intriguingly, it was revealed that AfRafA and AfStuA exert an antagonistic role in the regulation of biosynthesis of cyclopiazonic acid. In
Biological Activities of the Alkaloid Quinazoline Extracted from Aspergillus nomius
TWENTY FIVE Aspergillus isolates were screened from Giza Governorate and Saint Catherine Protectorate soils in Egypt. The antimicrobial activity of the crude extracts was tested against two Gram positive bacteria (Bacillus subtilis NRRL-B-4219, Staphylococcus aureus ATCC29213), four Gram negative bacteria (Alcaligenes faecalis B-170, Escherichia coli ATCC25922, Klebsiella pneumoniae ATCC10131, Pseudomonas aeruginosa ATCC27953), and one yeast (Candida albicans ATCC10231). The antioxidant activity using free radical scavenging model was assayed for the crude extracts. The antitumor activity for all of crude extracts was determined against HCT116 (Colon carcinoma cell line), HEPG2 (Liver carcinoma cell line), and MCF-7 (Breast carcinoma cell line). Aspergillus nomius was the most potent fungal species accordingly, it was chosen for bioactivity assay. Identification of this species was further confirmed at the molecular level based on nuclear ribosomal DNA 18s identities. An accession number, LC199488, was
3gly - Proteopedia, life in 3D
The refined crystal structures of a proteolytic fragment of glucoamylase from Aspergillus awamori var. X100 have been determined at pH 6 and 4 to a resolution of 2.2 A and 2.4 A, respectively. The models include the equivalent of residues 1 to 471 of glucoamylase from Aspergillus niger and a complete interpretation of the solvent structure. The R-factors of the pH 6 and 4 structures are 0.14 and 0.12, respectively, with root-mean-square deviations of 0.014 A and 0.012 A from expected bondlengths. The enzyme has the general shape of a doughnut. The hole of the doughnut consists of a barrier of hydrophobic residues at the center, which separates two water-filled voids, one of which serves as the active site. Three clusters of water molecules extend laterally from the active site. One of the lateral clusters connects the deepest recess of the active site to the surface of the enzyme. The most significant difference in the pH 4 and 6 structures is the thermal parameter of water 500, the putative ...
Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation<...
TY - JOUR. T1 - Bacteria-induced natural product formation in the fungus Aspergillus nidulans requires Saga/Ada-mediated histone acetylation. AU - Nuetzmann, Hans-Wilhelm. AU - Reyes-Dominguez, Yazmid. AU - Scherlach, Kirstin. AU - Schroeckh, Volker. AU - Horn, Fabian. AU - Gacek, Agnieszka. AU - Schümann, Julia. AU - Hertweck, Christian. AU - Strauss, Joseph. AU - Brakhage, Axel A. PY - 2011/8/23. Y1 - 2011/8/23. N2 - Sequence analyses of fungal genomes have revealed that the potential of fungi to produce secondary metabolites is greatly underestimated. In fact, most gene clusters coding for the biosynthesis of antibiotics, toxins, or pigments are silent under standard laboratory conditions. Hence, it is one of the major challenges in microbiology to uncover the mechanisms required for pathway activation. Recently, we discovered that intimate physical interaction of the important model fungus Aspergillus nidulans with the soil-dwelling bacterium Streptomyces rapamycinicus specifically ...
Production of α-L-Rhamnosidase from <em>Aspergillus flavus</em>: Optimization of Submerged Culture Conditions by Taguchi...
The enzyme α-L-rhamnosidase [E.C.3.2.1.40] specifically cleaves terminal α-L-rhamnose from a large number of natural products containing glycosides. This property provides this enzyme an important biotechnological application including removal of bitterness from citrus fruit juices, enhancement of grape wine aroma, removal of hesperidin crystals from orange juices, tomato pulp digestion and also help in conversion of clinical important steroids. In this study, production of α-L-rhamnosidase in submerged state fermentation from Aspergillus flavus was optimized using Taguchi DOE methodology. This statistical method provide the study of interaction of a large number of factors at different levels settings with a small number of experimental runs which leads to considerable economy in time and cost for the process optimization. The objective of this research was to determine the significant parameters for the production of α-L-rhamnosidase by Aspergillus flavus in submerged
Mycotaxon: Volume 78 Article Abstract
Moraes, A. M. L., M. Corrado, V. L. Holanda, G. L. Costa, M. Ziccardi, R. de Lourenço-de-Oliveira & P. C. Oliveira. Aspergillus from Brazilian mosquitoes - I. Genera Aedes and Culex from Rio de Janiero State, Mycotaxon 78: 413-422. 2001.. ABSTRACT: As part of an ongoing survey of potential biological control fungi from insects in Brazil. aspergilli were isolated from 625 mosquitoes belonging to 17 species in the genera Aedes and Culex. Two hundred and fifteen isolates belonging to 17 Aspergillus species were found. Aspergillus niger, Aspergillus flavus and Aspergillus nidulans were the most frequently isolated species. Two other species, Aspoergillus fischerianus and Aspergillus heteromorphus were new records for insects. The macro- and microcharacteristics of the Brazilian isolates are compared to those in previously published descriptions. The habitat and geographical distribution of these fungi in Brazil is reviewed.. KEYWORDS: biological control, taxonomy, entomogenous fungi. NOMENCLATURAL ...
Bola fúngica por Aspergillus fumigatus em cavidade pleural
Objective: To report the cases of 6 patients with fungus ball caused by Aspergillus fumigatus (aspergilloma) in the pleural cavity. Methods: Between 1980 and 2009, 391 patients were diagnosed with aspergilloma at the Santa Casa Hospital Complex in Porto Alegre, Brazil. The diagnosis of aspergilloma in the pleural cavity was made through imaging tests revealing effusion and pleural thickening with air-fluid level; direct mycological examination revealing septate hyphae, consistent with Aspergillus sp.; and positive culture for A. fumigatus in the surgical specimen from the pleural cavity. Results: Of the 391 patients studied, 6 (2%) met the established diagnostic criteria. The mean age of those 6 patients was 48 years (range, 29-66 years), and 5 (83%) were male. The most common complaints were cough, expectoration, and hemoptysis. Four patients (67%) had a history of tuberculosis that had been clinically cured. All of the patients were submitted to surgical removal of the aspergilloma, followed ...
A trispecies Aspergillus microarray: Comparative transcriptomics of three Aspergillus species | PNAS
In an application of the presented high-density microarray, we identify a carbon source-based response conserved in three aspergilli. The design of the study involving three different species, grown on three different defined minimal media, at three different values of pH, increases the likelihood of the found genes to be the true conserved core response to growth on xylose and not responses relying on an extra factor in addition to xylose (with the possible exception of abundant oxygen). We also believe this approach validates our argument that the xylanolytic transcriptional activator XlnR is a conserved system, even though it has not previously been studied in A. nidulans. Backed by the finding that the 5′-GGNTAAA-3′ motif is present and in some cases conserved as syntenic regions in all three species, we propose that the motif is indeed a XlnR motif and conserved in A. nidulans, A. niger, and A. oryzae. As a point of interest, a study of the homologous genes and their promoter regions ...
Asian Science Citation Index - Articles written by M.T. Hoque
The aim of present study was to investigate the bioactivity (antibacterial, antifungal and cytotoxicity etc.) of the ethylacetate extract isolated from Streptomyces sp. Aspergillus sp. and Bacillus sp. from sewage of different regions of Bangladesh. The ethylacetate extract of Streptomyces sp., Aspergillus sp. and Bacillus sp. shown modest antibacterial and antifungal activities at the concentration of 200 μg disc-1. The maximum antibacterial and antifungal activities were shown by the Streptomyces sp. The minimum inhibitory concentration of the microbial extracts was between 64 to 128 μg mL-1 against test organisms. Brine srimp lethality bioassay was carried out for cytotoxicity measurement of the extracts and the LC50 values were calculated after probit transformation of the resulting mortality data. All the ethyl acetate extract showed lower cytotoxicity properties (Streptomyces sp. 42.37 μg mL-1, Aspergillus sp. 52.25 μg mL-1 and Bacillus sp. 47.51 μg mL-1) compared with the reference ...
CiNii Articles - UCHIDA Kinji
Further evidence on the interspecific protoplast fusion between Aspergillus oryzae and Aspergillus sojae and subsequent haploidization, with special Reference to their production of some hydrolyzing enzymes. (1990 ...
Emergence of Azole Resistance in Aspergillus<...
TY - JOUR. T1 - Emergence of Azole Resistance in Aspergillus. AU - Wiederhold, Nathan. AU - Patterson, Thomas F. PY - 2015/10/25. Y1 - 2015/10/25. N2 - Resistance to the azole antifungals itraconazole, voriconazole, and posaconazole in Aspergillus species is a growing concern. This is especially alarming for A. fumigatus, where acquired resistance has been documented in patients with invasive disease caused by this species that were exposed to these agents, as well as in azole-naive individuals. The primary mechanisms of resistance that have been described in clinical strains include different point mutations in the CYP51A gene, which encodes the enzyme responsible for converting lanosterol to ergosterol via demethylation. Some resistant isolates also contain a tandem repeat in the promoter region of this gene that causes increased expression. These mutations, including TR34/L98H and TR46/Y121F/T289A have also been found in the environment in several areas of the world and have been demonstrated ...
RCSB PDB - 1IB4: Crystal Structure of Polygalacturonase from Aspergillus Aculeatus at Ph4.5 Structure Summary Page
1IB4: The X-ray structure of Aspergillus aculeatus polygalacturonase and a modeled structure of the polygalacturonase-octagalacturonate complex.
SWISS-MODEL Repository | A1C8U0
SWISS-MODEL Repository entry for A1C8U0 (MANF_ASPCL), Mannan endo-1,4-beta-mannosidase F. Aspergillus clavatus (strain ATCC 1007 / CBS 51365 / DSM 816 / NCTC 3887 /NRRL 1)
AID 1094518 - Antimicrobial activity against Aspergillus flavus at 500 ppm after 72 to 96 hr by disk diffusion method - PubChem
BioAssay record AID 1094518 submitted by ChEMBL: Antimicrobial activity against Aspergillus flavus at 500 ppm after 72 to 96 hr by disk diffusion method.
The Putative APSES Transcription Factor RgdA Governs Growth, Development, Toxigenesis, and Virulence in Aspergillus fumigatus |...
RgdA of A. fumigatus is a homolog of the Mbp1 protein of the budding yeast S. cerevisiae, a putative APSES TF. APSES TFs function as key regulators of fungal morphogenesis and development present in Aspergillus genomes, but only the stuA gene has been studied in A. fumigatus (10, 12, 14, 15, 17). The deletion of rgsA resulted in decreased mycelial growth and conidiation and reduced mRNA levels of key asexual development regulators compared to WT (Fig. 2). The mRNA levels of PKA signaling components were higher in the ΔrgdA strain than in WΤ and complemented strains. Furthermore, the ΔrgdA strain showed higher PKA activity in the absence of cAMP (Fig. 3), suggesting the presence of free PKA catalytic subunits in the cytoplasm (18). Based on these observations, we can propose that RgdA of A. fumigatus is necessary for normal growth and proper asexual development, which may involve regulation of the cAMP-PKA signaling pathway.. The Dpr proteins are known to be activated by the stress-activated ...
Women Health: Soy and Inflammatory disorders
According to the study of Anti-inflammatory effects of glyceollins derived from soybean by elicitation with Aspergillus sojae by Kim HJ, Sung MK, Kim JS., posted in US National Library of Medicine National Institutes of Health, researchers indicated that Glyceollins exert an anti-inflammatory effect, which is mediated through the inhibition of NF-κB activation in LPS-activated murine RAW264.7 cells. Glyceollins merit further study as potential therapeutic agents for inflammatory disorders ...