Bovine prothrombin contains three asparagine-linked sugar chains in 1 molecule. The sugar chains were quantitatively released from the polypeptide backbone by hydrazinolysis. All of the oligosaccharides thus obtained contain N-acetylneuraminic acid.

We have derived a novel method to assess compositional biases in biological sequences, which is based on finding the lowest-probability subsequences for a given residue-type set. As a case study, the distribution of prion-like glutamine/asparagine-rich ((Q+N)-rich) domains (which are linked to amyloidogenesis) was assessed for budding and fission yeasts and four other eukaryotes. We find more than 170 prion-like (Q+N)-rich regions in budding yeast, and, strikingly, many fewer in fission yeast. Also, some residues, such as tryptophan or isoleucine, are unlikely to form biased regions in any eukaryotic proteome.

A variety of unconventional translational and posttranslational mechanisms contribute to the production of antigenic peptides, thereby increasing the diversity of the peptide repertoire presented by MHC class I molecules. Here, we describe a class I-restricted peptide that combines several posttranslational modifications. It is derived from tyrosinase and recognized by tumor-infiltrating lymphocytes isolated from a melanoma patient. This unusual antigenic peptide is made of two noncontiguous tyrosinase fragments that are spliced together in the reverse order. In addition, it contains two aspartate residues that replace the asparagines encoded in the tyrosinase sequence. We confirmed that this peptide is naturally presented at the surface of melanoma cells, and we showed that its processing sequentially requires translation of tyrosinase into the endoplasmic reticulum and its retrotranslocation into the cytosol, where deglycosylation of the two asparagines by peptide-N-glycanase turns them into

Many prion-forming proteins contain glutamine/asparagine (Q/N) wealthy domains, and you can find conflicting opinions regarding the role of primary sequence in their conversion to the prion form: is this phenomenon driven primarily by amino acid composition, or, as a recent computational analysis suggested, dependent on the presence of short sequence elements with high amyloid-forming potential. formation in proteins that are rich in glutamine and asparagine are still under debate: is the process driven by primary sequence or by amino acid composition? In 2015 Sabate et al. published a paper suggesting that RNH6270 the process is triggered by short amyloid-prone sequences. Their argument was based on the success of their pWALTZ classifier, which uses a database of short peptides with known amyloid forming propensities. To explore the validity of their argument we compared their original scoring matrices with shuffled scoring matrices, and found no decrease in accuracy, suggesting that the ...

Translations: Asparagīna Amino Acid, Asparaginas aminorūgšties, Asparagina aminoacizi, Asparagin Aminokiselinska, Amino Acid Asparagine, Asparaginy aminokwasów, Asparagine एमिनो एसिड, Aminoácido asparagina, Аспарагин Аминокислоты, Ασπαραγίνη Αμινοξύ, الهليونين الأحماض الأمينية, 아스파라긴 아미노산, Asparagin aminokyselin, Asparagina Asam Amino, Asparagine Amino acid, 天冬酰胺氨基酸, Asparagina Aminoàcids, Asparagin Amino Acid, Asparagín aminokyselín, Amminoacido asparagina, אספרגין חומצה אמינו, Asparagin Amino Acid, Аспарагин амино киселина, アスパラギンアミノ酸, Acide aminé asparagine, Asparagin Amino Acid, Asparagin Amino Acid, Asparaginer Amino Acid, Asparagina Aminoácidos, Аспарагін Амінокислоти, Asparagiini Aminohappo, Аспарагин амино ...

The structure of synC can be divided into three subdomains (Figure 1D): the amino‐terminal subdomain (residues 110‐223), the central subdomain (residues 246‐308) and the carboxy‐terminal subdomain (residues 224‐245 and 309‐420). The core of the amino‐terminal subdomain is a four‐stranded parallel β‐sheet (β1, β2, β6, β7), which is flanked by a helical loop (residues 124‐137) and helix α1. It resembles the nucleotide‐binding motif (NAD motif) found in numerous enzymes that bind substrates at the carboxyl end of the parallel β‐sheet. The parallel arrangement of helices α1 and α2 and their connection by strand β7 are remarkably well conserved in synC and GSHase. Even more remarkable is the existence of a cis‐asparagine residue at the carboxyl end of strand β7 in both synC (Asn214) and GSHase (Asn114). As the cis conformation is extremely rare in peptide bonds preceding residues other than proline, the use of cis‐asparagine at equivalent positions in synC and ...

Although bafilomycin induced HIF-1α levels under normoxic conditions, it has little effect on the expressions of HIF-1 targeted genes. This effect of bafilomycin on HIF-1α could be suspected of the factor-inhibiting HIF (FIH). In the absence of a hypoxic signal, HIF-1α can be inactivated by FIH. FIH hydroxylates an asparagine residue (Asn803) within the transactivation domain of HIF-1α, which blocks its recruitment of p300 coactivator (Lando et al., 2002). Moreover, under hypoxic conditions, asparagine hydroxylation is inhibited due to limited oxygen, and HIF-1α remains unmodified and activated. This seems to be one of the reasons why HIF-1α induced by bafilomycin has little transcriptional activity; similarly, HIF-1α induced by heat shock was found to have no transcriptional activity (Katschinski et al., 2002). However, HIF-1α induced by bafilomycin induced p21 transcription. Recently, Koshiji et al. (2004) demonstrated the mechanism of p21 induction by HIF-1α (i.e., c-Myc represses ...

This sequence change replaces asparagine with serine at codon 162 of the BBS2 protein (p.Asn162Ser). The asparagine residue is highly conserved and there is a small physicochemical difference between asparagine and serine. This variant is present in population databases (rs749148042, ExAC 0.01%). This variant has not been reported in the literature in individuals with BBS2-related conditions. Algorithms developed to predict the effect of missense changes on protein structure and function are either unavailable or do not agree on the potential impact of this missense change (SIFT: Tolerated; PolyPhen-2: Possibly Damaging; Align-GVGD: Class C0). In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance ...

The amino acid asparagine was until now considered non-essential because it is produced naturally by the body. However, researchers found that it is essential for normal brain development.

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The researchers narrow it down to a single change in the outer coat of the virus. S139N - the switch in amino acids from serine to asparagine at position 139 in the membrane protein (PrM). This change was sufficient to help Zika virus infect brain cells from both humans and mice efficiently. Contributing to an unprecedented outbreak.. Reference. 1. A single mutation in the prM protein of Zika virus contributes to fetal microcephaly.Yuan L, Huang XY, Liu ZY, Zhang F, Zhu XL, Yu JY, Ji X, Xu YP, Li G, Li C, Wang HJ, Deng YQ, Wu M, Cheng ML, Ye Q, Xie DY, Li XF, Wang X, Shi W, Hu B, Shi PY, Xu Z, Qin CF. Science. 2017 Sep 28. pii: eaam7120. doi: 10.1126/science.aam7120.. ...

This sequence change replaces histidine with asparagine at codon 6717 of the SYNE1 protein (p.His6717Asn). The histidine residue is highly conserved and there is a small physicochemical difference between histidine and asparagine. This variant is not present in population databases (ExAC no frequency). This variant has not been reported in the literature in individuals with SYNE1-related conditions. Algorithms developed to predict the effect of missense changes on protein structure and function (SIFT, PolyPhen-2, Align-GVGD) all suggest that this variant is likely to be disruptive, but these predictions have not been confirmed by published functional studies and their clinical significance is uncertain. In summary, the available evidence is currently insufficient to determine the role of this variant in disease. Therefore, it has been classified as a Variant of Uncertain Significance ...

Alg6 - Alg6 (untagged ORF) - Rat asparagine-linked glycosylation 6, alpha-1,3-glucosyltransferase homolog (S. cerevisiae) (Alg6), (10 ug) available for purchase from OriGene - Your Gene Company.

Asparagine is an amino acid, which has recently been rumored to aid the spread of breast cancer. Trials on mice have shown that low-asparagine diets, combined with blocking asparagine production in the body, greatly reduced the breast cancers ability to spread.. ...

This gene encodes an enzyme that catalyzes hydrolysis of an N(4)-(acetyl-beta-D-glucosaminyl) asparagine residue to N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue. The encoded enzyme may play a role in the proteasome-mediated degradation of misfolded glycoproteins. Multiple transcript variants encoding different isoforms have been found for this gene ...

Looking for online definition of Asparagine-linked glycosylation protein 6 homolog in the Medical Dictionary? Asparagine-linked glycosylation protein 6 homolog explanation free. What is Asparagine-linked glycosylation protein 6 homolog? Meaning of Asparagine-linked glycosylation protein 6 homolog medical term. What does Asparagine-linked glycosylation protein 6 homolog mean?

The asparaginyl hydroxylase FIH [factor inhibiting HIF (hypoxia-inducible factor)] was first identified as a protein that inhibits transcriptional activation by HIF, through hydroxylation of an asparagine residue in the CAD (C-terminal activation domain). More recently, several ARD [AR (ankyrin repeat) domain]-containing proteins were identified as FIH substrates using FIH interaction assays. Although the function(s) of these ARD hydroxylations is unclear, expression of the ARD protein Notch1 was shown to compete efficiently with HIF CAD for asparagine hydroxylation and thus to enhance HIF activity. The ARD is a common protein domain with over 300 examples in the human proteome. However, the extent of hydroxylation among ARD proteins, and the ability of other members to compete with HIF-CAD for FIH, is not known. In the present study we assay for asparagine hydroxylation in a bioinformatically predicted FIH substrate, the targeting subunit of myosin phosphatase, MYPT1. Our results confirm hydroxylation

Activity of the hypoxia-inducible factor (HIF) complex is controlled by oxygen-dependent hydroxylation of prolyl and asparaginyl residues. Hydroxylation of specific prolyl residues by 2-oxoglutarate (2-OG)-dependent oxygenases mediates ubiquitinylation and proteasomal destruction of HIF-alpha. Hydroxylation of an asparagine residue in the C-terminal transactivation domain (CAD) of HIF-alpha abrogates interaction with p300, preventing transcriptional activation. Yeast two-hybrid assays recently identified factor inhibiting HIF (FIH) as a protein that associates with the CAD region of HIF-alpha. Since FIH contains certain motifs present in iron- and 2-OG-dependent oxygenases we investigated whether FIH was the HIF asparaginyl hydroxylase. Assays using recombinant FIH and HIF-alpha fragments revealed that FIH is the enzyme that hydroxylates the CAD asparagine residue, that the activity is directly inhibited by cobalt(II) and limited by hypoxia, and that the oxygen in the alcohol of the hydroxyasparagine

Asparagine (Asn) is one of the 20 most common natural amino acids on Earth. It has carboxamide as the side chains functional group. Asparagine is not an essential amino acid, which means that it can be synthesized from central metabolic pathway intermediates in humans and is not required in the diet. The precursor to asparagine is oxaloacetate. Oxaloacetate is converted to aspartate using a transaminase enzyme. The enzyme transfers the amino group from glutamate to oxaloacetate producing alpha-ketoglutarate and aspartate. The enzyme asparagine synthetase produces asparagine, AMP, glutamate, and pyrophosphate from aspartate, glutamine, and ATP. In the asparagine synthetase reaction, ATP is used to activate aspartate, forming beta-aspartyl-AMP. Glutamine donates an ammonium group which reacts with beta-aspartyl-AMP to form asparagine and free AMP. Since the asparagine side chain can make efficient hydrogen bond interactions with the peptide backbone, asparagines are often found near the beginning ...

Background Polyglutamine expansion is responsible for several neurodegenerative disorders, among which Huntington disease is the most well-known. Studies in the yeast model demonstrated that both aggregation and toxicity of a huntingtin (htt) protein with an expanded polyglutamine region strictly depend on the presence of the prion form of Rnq1 protein ([PIN+]), which has a glutamine/asparagine-rich domain. Principal Findings Here, we showed that aggregation and toxicity of mutant htt depended on [PIN+] only quantitatively: the presence of [PIN+] elevated the toxicity and the levels of htt detergent-insoluble polymers. In cells lacking [PIN+], toxicity of mutant htt was due to the polymerization and inactivation of the essential glutamine/asparagine-rich Sup35 protein and related inactivation of another essential protein, Sup45, most probably via its sequestration into Sup35 aggregates. However, inhibition of growth of [PIN+] cells depended on Sup35/Sup45 depletion only partially, suggesting that

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Asparagine. Molecular model of the amino acid asparagine. Its chemical formula is C4.H8.N2.O3. Atoms are represented as spheres and are colour- coded: carbon (blue), hydrogen (gold), oxygen (red) and nitrogen (dark blue). Amino acids are the building blocks of proteins. Asparagine is a non-essential amino acid. It can be synthesised by the body and so does not need to come from the diet. Asparagine plays an important role in the folding of protein molecules into their secondary structures (alpha helices and beta sheets). Some proteins (such as haemoglobin, the oxygen- carrying pigment in human blood) cannot function without being folded into the correct shape. - Stock Image A611/0042

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Asparagine-linked sugar chains of glycoproteins in calf thymocyte plasma membrane. Isolation and fractionation of oligosaccharides liberated by hydrazinolysis.:Isolation and Fractionation of Oligosaccharides Liberated by Hydrazinolysis （1980 ...

The mechanism by which asparaginase inhibits the 6C3HED tumor is probably not directly related to the cellular concentration of asparagine, since the concentration of asparagine decreases in resistant tumors, spleen, and liver, as well as in susceptible tumors. Amino acids other than asparagine may be affected by asparaginase because of the variety of metabolic relationships of the amino acids. In this study, analyses of all the amino acids of resistant 6C3HED tumor, susceptible 6C3HED tumor, spleen, and liver were undertaken.. Most of the amino acids of the susceptible tumor increased from 2- to 40-fold, while only slight changes occurred in the resistant tumor. The most dramatic change was a decrease in glycine in the susceptible tumor. No parallel change in glycine occurred in the resistant tumor, the spleen, or the liver. It is suggested that the susceptible tumor may synthesize glycine from asparagine; consequently, the decrease in asparagine is followed by a decrease in cellular glycine. ...

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The researchers narrow it down to a single change in the outer coat of the virus. S139N - the switch in amino acids from serine to asparagine at position 139 in the membrane protein (PrM). This change was sufficient to help Zika virus infect brain cells from both humans and mice efficiently. Contributing to an unprecedented outbreak.. Reference. 1. A single mutation in the prM protein of Zika virus contributes to fetal microcephaly.Yuan L, Huang XY, Liu ZY, Zhang F, Zhu XL, Yu JY, Ji X, Xu YP, Li G, Li C, Wang HJ, Deng YQ, Wu M, Cheng ML, Ye Q, Xie DY, Li XF, Wang X, Shi W, Hu B, Shi PY, Xu Z, Qin CF. Science. 2017 Sep 28. pii: eaam7120. doi: 10.1126/science.aam7120.. ...

Most core residues of coiled coils are hydrophobic. Occasional polar residues are thought to lower stability, but impart structural specificity. The coiled coils of trimeric autotransporter adhesins (TAAs) are conspicuous for their large number of polar residues in position d of the core, which often leads to their prediction as natively unstructured regions. The most frequent residue, asparagine (N@d), can occur in runs of up to 19 consecutive heptads, frequently in the motif [I/V]xxNTxx. In the Salmonella TAA, SadA, the core asparagines form rings of interacting residues with the following threonines, grouped around a central anion. This conformation is observed generally in N@d layers from trimeric coiled coils of known structure. Attempts to impose a different register on the motif show that the asparagines orient themselves specifically into the core, even against conflicting information from flanking domains. When engineered into the GCN4 leucine zipper, N@d layers progressively ...

Product Number , 75190706. CAS Number , 5794-24-1. EC , 218-163-3. Molecular Formula , C4H8N2O3Ï H2O. Molecular Weight , 150.14. Storage Temp , +20°C. Harmonized Tariff code , 29241900900. Signal Word , ...

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BioAssay record AID 253508 submitted by ChEMBL: Concentration of compound inhibiting Lys103-Asn mutant HIV-1(IIIB) induced cytopathicity in CEM cell culture by 50%.

Research into the chemical biology of bromodomains has been driven by the development of acetyl-lysine mimetics. The ligands are typically anchored by binding to a highly conserved asparagine residue. Atypical bromodomains, for which the asparagine is mutated, have thus far proven elusive targets, including

Zotik an jî anûs di anatomî de ew qulika dervayî ji rektum (ji latînî hatiye, ango rast). Zotik beşeke navende di qûnê mirov de û beşa dawî ji sîstema givêrdarê (hezmê) de ku mayînên laşê wek gû (feces bi Înglîzî) ji têne avêtin ku ji bi Înglîzî defaction tê gotin, û ev jî erkeke bingeh û gelek pêwîste ji erkên (fanksiyonên) zûtik û qûn di laşê mirov de. Zotik roleke di zayenditî de dilîze di kirdarê anal seks ango kirdarê zayendî bi riya qûn yan qûnetî (lotî) ku li pir civaktiyên wek civaktiyên misilmana gune ye û dijî olê Îslamê ye. Bi riya zûtik çend înfeksiyonan peyda dibe wek şêrpençîr (cancer). Bo tendirustiya zûtik û qûn pakî (paqijî) divê bi av û sabûn pey destavê û di serşûştinê ne bi pelê tuwelêtê ku baş paqij nake. ...

Aspartate-Ammonia Ligase: An enzyme that catalyzes the formation of asparagine from ammonia and aspartic acid, in the presence of ATP. EC 6.3.1.1.

அஸ்பரஜின் (Asparagine) (அ) அஸ்பரமைடு [குறுக்கம்: Asn (அ) N; அஸ்பார்டிக் அமிலம் (அ) அஸ்பரஜின் அமினோ அமிலத்தை குறிக்கும் மற்றொரு குறுக்கம்: Asx or B][2] புரதங்களில் அடிப்படையாக உள்ள 20 அமினோ அமிலங்களில் ஒன்றாகும். இதனுடைய வாய்பாடு: 2HN-CO-CH2-CH(NH2)-COOH (அ) C4H8N2O3. இது ஒரு அத்தியாவசியமற்ற ஆல்ஃபா அமினோ அமிலமாகும். அஸ்பரஜின் விலங்குகளினால்/மனிதர்களால் தயாரிக்கப்படக்கூடியது. இதன் குறிமுறையன்கள்: AAU மற்றும் AAC. ...

Hydrolysis of an N(4)-(acetyl-beta-D- glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl- beta-D-glucosaminylamine and a peptide containing an aspartate residue ...

1NJE: Partitioning roles of side chains in affinity, orientation, and catalysis with structures for mutant complexes: asparagine-229 in thymidylate synthase.

1NJB: Partitioning roles of side chains in affinity, orientation, and catalysis with structures for mutant complexes: asparagine-229 in thymidylate synthase.

II. LYMPHOMA 6C3HED CELLS CULTURED IN A MEDIUM DEVOID OF L-ASPARAGINE LOSE THEIR SUSCEPTIBILITY TO THE EFFECTS OF GUINEA PIG SERUM IN VIVO ...

Po e ndjekim hap pas hapi ecurinë e analizës së këtij shkruesi që lodhet shumë pa e ditur mirë se çfarë po shkruan, meqë ende nuk i ka fituar aftësitë e mundësitë e duhura me atë përvojë të pakët profesionale në fushën e letërsisë shqipe, e cila është një shkencë serioze dhe me kontribute të lashta sasiore e cilësore. Mbasi vlerat e saja estetiko-artistike janë të njohura me kohë kombëtarisht e botërisht. Le tua lëmë fjalën pjesëve problematike e thelbësore të këtij shkrimi. Shkruesi që në fillim arsyeton, me një analizë jo të thelluar dhe mjaft sipërfaqësore e hera-herës me një kuptim të kundërt me çfarë kumton përmbajtja e poezive të këtij autori, duke e krahasuar përmbajtjen e tyre me disa fjalë të huaja. Konkretisht ai shprehet:Ditarët e mjegullës, një metaforë që në profecinë e saj paralajmëron...ngulmimet e poetit...në imazherinë e shoqërisë e të hapë rrugëkalimet drejt një prosperiteti të dëshirueshëm nën ...

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Complete Information about ASN 131258 including Assigned Organization, IP range count, and Country range count. Research all IP ranges within ASN 131258 including specific CIDR data, Subnets, and IP address location information.

سیانوباکترها یک گروه منحصر به فرد از باکتری-های فتواتوتروف هستند که برخی از آن‌ها به دلیل ویژگی-های ساختاری، تحمل قابل توجهی به تنش شوری نشان می-دهند. این موجودات نقش مهمی در محیط-های خاکی به ویژه در نواحی خشک و نیمه-خشک ایفا می-کنند. در مطالعه حاضر سیانوباکترهای خاک از مناطق بیابانی ایران جداسازی شده و سپس جدایه-های مقاوم به شرایط فوق شور شناسایی شدند. 40 نمونه خاک از پارک ملی کویر تهیه شد. نمونه-ها پس از کشت در محیط BG11 و ASN III (5/3، 5، 6 و 7 درصد کلرید سدیم) و انکوباسیون در شرایط مناسب دمایی و نور، جداسازی شده و با استفاده از کلیدهای مورفولوژیکی به طور اولیه و سپس با

TY - JOUR. T1 - Amino Acid Templating of Inorganic Networks. T2 - Synthesis and Structure of L-Asparagine Zinc Phosphite, C4N2O3H 8·ZnHPO3. AU - Gordon, Laura E.. AU - Harrison, William T.A.. PY - 2004/3/22. Y1 - 2004/3/22. N2 - C4N2O3H8·ZnHPO 3 is the first zincophosphite framework to be templated by an amino acid (L-asparagine), which bonds to Zn via a carboxyl O atom. It contains infinite, homochiral, helical 4-ring chains of ZnO4 and HPO 3 groups, stabilized by intra- and interchain N-H⋯O hydrogen bonds. Crystal data: C4N2O3H 8·ZnHPO3, Mr = 277.49, orthorhombic, P212121 (No. 19), a = 5.0349(2) Å, b = 9.4539(4) Å, c = 18.6092(8) Å, V = 885,79 (6) Å3, Z = 4.. AB - C4N2O3H8·ZnHPO 3 is the first zincophosphite framework to be templated by an amino acid (L-asparagine), which bonds to Zn via a carboxyl O atom. It contains infinite, homochiral, helical 4-ring chains of ZnO4 and HPO 3 groups, stabilized by intra- and interchain N-H⋯O hydrogen bonds. Crystal data: C4N2O3H 8·ZnHPO3, Mr = ...

The antileukemic activity of l-asparaginase (ASNase), an important component of therapy for acute lymphoblastic leukemia, is thought to result from depletion of serum l-asparagine (Asn). In studies of the pharmacological effects of ASNase, investigators have reported prolonged reduction in the serum concentration of Asn after the administration of ASNase. Such measurements may not be valid because ASNase present in the blood sample may hydrolyze Asn before its determination. We examined recovery of [U-14C]Asn from blood samples with and without various concentrations of added ASNase. In the presence of ≥0.01 IU/ml of ASNase, the amount of [U-14C]Asn recovered was ,15% of that without ASNase. Utilizing this assay, we studied the effect of 2 known inhibitors of ASNase in an attempt to improve Asn recovery. In the presence of aspartic β semialdehyde (ASA), or 5-diazo-4-oxo-l-norvaline (DONV), and up to 1.0 IU/ml ASNase, Asn levels remained at ,90% of control. ASA prevented the hydrolysis of ...

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Targeting amino acid metabolism has therapeutic implications for aggressive brain tumors. Asparagine is an amino acid that is synthesized by normal cells. However, some cancer cells lack asparagine synthetase (ASNS), the key enzyme for asparagine synthesis. Asparaginase (ASNase) contributes to eradication of acute leukemia by decreasing asparagine levels in serum and cerebrospinal fluid. However, leukemic cells may become ASNase-resistant by up-regulating ASNS. High expression of ASNS has also been associated with biological aggressiveness of other cancers, including gliomas. Here, the impact of enzymatic depletion of asparagine on proliferation of brain tumor cells was determined. ASNase was used as monotherapy or in combination with conventional chemotherapeutic agents. Viability assays for ASNase-treated cells demonstrated significant growth reduction in multiple cell lines. This effect was reversed by glutamine in a dose-dependent manner -- as expected, because glutamine is the main amino ...

Sugar moieties on the cell surface play one of the most important roles in cellular recognition. In order to elucidate the molecular mechanism of these cellular phenomena, assessment of the structure...

A sequence of about forty amino-acid residues found in epidermal growth factor (EGF) has been shown [ (PUBMED:2288911) (PUBMED:6334307) (PUBMED:3534958) (PUBMED:6607417) (PUBMED:3282918) ] to be present in a large number of membrane-bound and extracellular, mostly animal, proteins. Many of these proteins require calcium for their biological function and a calcium-binding site has been found at the N terminus of some EGF-like domains [ (PUBMED:1527084) ]. Calcium-binding may be crucial for numerous protein-protein interactions. For human coagulation factor IX it has been shown [ (PUBMED:7606779) ] that the calcium-ligands form a pentagonal bipyramid. The first, third and fourth conserved negatively charged or polar residues are side chain ligands. The latter is possibly hydroxylated (see aspartic acid and asparagine hydroxylation site) [ (PUBMED:1527084) ]. A conserved aromatic residue, as well as the second conserved negative residue, are thought to be involved in stabilising the calcium-binding ...

A sequence of about forty amino-acid residues found in epidermal growth factor (EGF) has been shown [ (PUBMED:2288911) (PUBMED:6334307) (PUBMED:3534958) (PUBMED:6607417) (PUBMED:3282918) ] to be present in a large number of membrane-bound and extracellular, mostly animal, proteins. Many of these proteins require calcium for their biological function and a calcium-binding site has been found at the N terminus of some EGF-like domains [ (PUBMED:1527084) ]. Calcium-binding may be crucial for numerous protein-protein interactions. For human coagulation factor IX it has been shown [ (PUBMED:7606779) ] that the calcium-ligands form a pentagonal bipyramid. The first, third and fourth conserved negatively charged or polar residues are side chain ligands. The latter is possibly hydroxylated (see aspartic acid and asparagine hydroxylation site) [ (PUBMED:1527084) ]. A conserved aromatic residue, as well as the second conserved negative residue, are thought to be involved in stabilising the calcium-binding ...

HIF plays a central role in the transcriptional response to changes in oxygen availability. The PHD family of oxygen-dependent prolyl hydroxylases plays a pivotal role in regulating HIF stability. The biochemical properties of these enzymes make them well suited to act as oxygen sensors. They also respond to other intracellular signals, including reactive oxygen species, nitric oxide, and certain metabolites, that can modulate the hypoxic response. HIF transcriptional activity is further tuned by FIH1-mediated asparagine hydroxylation. HIF affects signaling pathways that influence development, metabolism, inflammation, and integrative physiology. Accordingly, HIF-modulatory drugs are now being developed for diverse diseases.

Larsen TM, Boehlein SK, Schuster SM, Richards NG, Thoden JB, Holden HM, Rayment I (December 1999). Three-dimensional structure of Escherichia coli asparagine synthetase B: a short journey from substrate to product. Biochemistry. 38 (49): 16146-57. CiteSeerX 10.1.1.453.5998. doi:10.1021/bi9915768. PMID 10587437 ...

Suolinna, E; Tritsch, G; and Hakala, M T., Asparagine (asn) and glutamine (gln) metabolism in sarcoma 180 cells in vitro. Abstr. (1971). Subject Strain Bibliography 1971. 2397 ...

Asparagine is a nonessential amino acid, which means that it is manufactured from other amino acids in the liver; it does not have to be obtained directly through the diet.

Asparagine (abbreviated as Asn or N; Asx or B represent either asparagine or aspartic acid) is one of the 20 most common natural amino acids on Earth. It has carboxamide as the side chains functional group. It is not an essential amino acid. Its codons are AAU and AAC. A reaction between asparagine and reducing sugars or reactive carbonyls produces acrylamide (acrylic amide) in food when heated to sufficient temperature. These products occur in baked goods such as french fries, potato chips, and roasted coffee.

Asparagine synthetase antibody (asparagine synthetase (glutamine-hydrolyzing)) for IHC-P, WB. Anti-Asparagine synthetase pAb (GTX114269) is tested in Human samples. 100% Ab-Assurance.

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Background Voltage-dependent block from the NMDA receptor by Mg2+ is usually thought to be central to the unique involvement of this receptor in higher brain functions. hippocampal spatial info processing by attenuating activity-dependent synaptic potentiation in the dentate gyrus. overexpressing an NMDAR defective for Mg2+ block has problems in long-term memory space [7]. However, the role of the Mg2+ block in vertebrates still remains unclear. NMDARs defective for the Mg2+ block cause perinatal lethality in mice expressing these receptors [8]. A single amino acid substitution is known to greatly switch the Mg2+ blockade of the NMDAR. Practical NMDARs are likely hetero-oligomers comprising two types of subunits, GluN1 and GluN2. Each subunit offers four expected membrane-associated segments (M1CM4). A single asparagine residue in M2 is critical for voltage-dependent Mg2+ blockade. Alternative of asparagine 598 of the GluN1 subunit with glutamine strongly reduces the level of sensitivity of the ...

Alpha-1,2-mannosyltransferase, catalyzes sequential addition of the two terminal alpha 1,2-mannose residues to the Man5GlcNAc2-PP-dolichol intermediate during asparagine-linked glycosylation in the ...

Alg1 - Alg1 (untagged) - Mouse asparagine-linked glycosylation 1 homolog (yeast, beta-1,4-mannosyltransferase) (cDNA clone MGC:18946, (10ug) available for purchase from OriGene - Your Gene Company.

Lentivirus with CMV promoter-driven expression of asparagine-linked glycosylation 9, alpha-1,2-mannosyltransferase homolog (S. cerevisiae) (ALG9), transcript variant 1 in pLenti vector with puromycin selection and C-terminal Myc and FLAG tags.

Complete information for ASNS gene (Protein Coding), Asparagine Synthetase (Glutamine-Hydrolyzing), including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

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Acts only on asparagine-oligosaccharides containing one amino acid, i.e., the asparagine has free alpha-amino and alpha-carboxyl groups [cf. EC 3.5.1.52, peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase ...

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Creative Biolabs has established a platform predict and assess deamidation of antibody therapeutics as part of manufacturability assessment.

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ASN1_STRING_new() returns an allocated ASN1_STRING structure. Its type is undefined. ASN1_STRING_type_new() returns an allocated ASN1_STRING structure of type type. ASN1_STRING_free() frees up a. ...

Asparaginasefrom Escherichia coliL-Asparagine amidohydrolaseEC # 3.5.1.1If you need bigger quantities than indicated on the web site, please contact us for a personal offer.