Dive into the research topics of Mitogen-activated protein kinase phosphorylates and negatively regulates basic helix-loop-helix-PAS transcription factor BMAL1. Together they form a unique fingerprint. ...

Sigma-Aldrich offers abstracts and full-text articles by [Vikram R Shende, Marianna M Goldrick, Suchitra Ramani, David J Earnest].

Honor Morris is a PhD student in the Division of Cell Matrix Biology & Regenerative Medicine at the University of Manchester, working under the supervision of Prof. Judith Hoyland and Prof. Qing-Jun Meng. Her research seeks to address the role of intrinsic circadian rhythms in intervertebral disc homeostasis and degeneration. Honors current work focuses on the influence of clock gene BMAL1 on the ossification of cartilage tissues, and the development of circadian rhythms within the embryonic spine.. As part of the RUBICON exchange, Honor visited the laboratory of Prof. Danny Chan at the University of Hong Kong. Here she examined the development of the circadian clock within the mouse musculoskeletal system and studied growth plate and intervertebral disc phenotypes arising from circadian disruption.. ...

Animals. Mice were maintained at 20°C to 22°C under an LD schedule (light on at 7 am; light off at 7 pm), with ad libitum access to standard rodent chow. Bmal1fl/fl mice (24) were crossed onto a PER2::luc background. PER2::luc mice carry the firefly luciferase gene fused in-frame with the 3′ end of the Per2 gene, creating a fusion protein reporter (25). Bmal1fl/fl PER2::luc mice were subsequently crossed with Col2a1Cre mice expressing Cre recombinase under the control of the Col2a1 promoter (23) to generate cartilage-specific Bmal1-KO mice. All mice were bred in-house at the University of Manchester.. Genotyping of Col2a1-Bmal1-/- mice. DNA was extracted from the ear clips from Col2a1 Bmal1-/- mice using a QIAamp DNA Mini Kit (QIAGEN). The PCR reactions were carried out using a Bmal1 forward primer (5′-CTCCTAACTTGGTTTTTGTCTGT-3′) and a Bmal1 reverse primer (5′-CTGACCAACTTGCTAACAATTA-3′) with BioMix Red reaction mix (Bioline). The PCR amplification was performed in an Eppendorf ...

Adipocytes play multiple roles in energy balance: they store and release energy and also provide signals to the CNS about energy storage. One example of the importance of white adipose tissue in energy balance involves release of fatty acids into the circulation. When this occurs, fatty acid concentrations increase in the hypothalamus-a change that results in signals that decrease food intake. Growing interest in circadian control of the systems underpinning appetite and feeding led to the observation that mice with germline knockout of Arntl (a gene encoding a key molecular clock element) are heavier and have more adipose tissue than their wild-type counterparts and that these differences become evident as early as 4-8 weeks. A new report by Paschos et al. takes this line of investigation further by determining the impact on weight and food intake when Arntl is specifically deleted from adipocytes. The investigators demonstrate that in contrast to mice in which Arntl is deleted in hepatocytes ...

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In mice, mutations or targeted disruptions of the core circadian gene Bmal1 have been implicated in early onset of ocular pathologies, including premature/congenital cataract development. The aim of the present study was to analyze probands of consanguineous Pakistani cataract families to identify the novel pathogenic variants in the BMAL1 gene. We have studied 21 congenital cataract families. Ophthalmic examination was performed for the probands and available family members. Genomic DNA was isolated from peripheral blood. PCR and Sanger sequencing was performed for the entire coding region of the BMAL1 gene. Targeted Sanger sequencing of BMAL1 revealed a heterozygous variant c.41A>T; p.(Asp14Val) in one proband, but it did not co-segregate with the disease phenotype in the family. In addition, a nonsynonymous variant (rs2290037) was identified in five probands. Our study is the first one to analyze the role of BMAL1 gene mutations in humans for their association with congenital cataract. Although we

Jujube also contains a flavonoid compound, spinosin, which appears to trigger sleepiness through its effects on serotonin. Valerian-its, actually the root of the valerian plant that is used medicinally-boosts the levels of calming neurotransmitters in the brain, including GABA, a neurochemical that is critical for healthy sleep. While melatonin is a hormone found in the human body, valerian root certainly is not, therefore it works in a much different way. have taken two benadryl and 2 Ambien so far. Research has shown that the omega-3 fatty acid DHA reduces disruptions to the circadian clock gene Bmal1, one of several core clock genes that keep circadian clocks and rhythms functioning in sync. I have had no side effects. These two systems work alongside one another to regulate sleep and wakefulness throughout the night, to ensure we get the rest we need AND have the alertness, energy and focus we require to perform in our daily lives. Medically reviewed by Drugs.com on Sep 29, 2020 - Written ...

Mammalian circadian rhythms are generated by a feedback loop in which BMAL1 and CLOCK, players of the positive limb, activate transcription of the cryptochrome and period genes, components of the negative limb. Bmal1 and Per transcription cycles display nearly opposite phases and are thus governed b …

ARNTL - ARNTL (untagged)-Human aryl hydrocarbon receptor nuclear translocator-like (ARNTL), transcript variant 1 available for purchase from OriGene - Your Gene Company.

Circadian oscillators are expressed in various peripheral tissues including the ovary and uterus and may play critical roles in the regulation of reproductive physiological processes (Dolatshad et al. 2006; He et al. 2007a; Hirata et al. 2009; Akiyama et al. 2010; Uchikawa et al. 2011). To address these possible roles, we focused on the function of the circadian oscillators in the expression of Gdf family members by using rat UESCs. The results indicate that a few Gdf family members are repressed by the binding of REV‐ERBα, a canonical clock component, to the RORE sites located at Gdf putative promoters. We found that the expression levels of Gdf10 and Gdf15 were significantly increased in the embryo implantation sites of the pregnant D6.5 rat uterus compared to that at pregnant D4.5. We and other research group also observed that the canonical clock genes Bmal1, Rev‐erbα, and Per2 were downregulated in UESCs during the in vitro decidualization (Isayama et al. 2015; Muter et al. 2015; ...

Expression of ARNTL2 (bHLHe6, BMAL2, CLIF, MOP9, PASD9) in smooth muscle tissue. Antibody staining with HPA059074 in immunohistochemistry.

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5-[A/G]GGTCA-3 preceded by an A/T-rich 5 sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two

INOUE Ikuo , HAYASHI Kenji , YAGASAKI Fumiharu , NAKAMURA Koh-ichi , MATSUNAGA Toshiyuki , XU Haiyuan , INUKAI Koh-ichi , AWATA Takuya , KOMODA Tsugikazu , KATAYAMA Shigehiro Journal of atherosclerosis and thrombosis 10(2), 99-108, 2003-04-01 医中誌Web 参考文献23件 被引用文献4件 ...

In liver, most metabolic pathways are under circadian control, and hundreds of protein-encoding genes are thus transcribed in a cyclic fashion. Here we show that rhythmic transcription extends to the locus specifying miR-122, a highly abundant, hepatocyte-specific microRNA. Genetic loss-of-function and gain-of-function experiments have identified the orphan nuclear receptor REV-ERBalpha as the major circadian regulator of mir-122 transcription. Although due to its long half-life mature miR-122 accumulates at nearly constant rates throughout the day, this miRNA is tightly associated with control mechanisms governing circadian gene expression. Thus, the knockdown of miR-122 expression via an antisense oligonucleotide (ASO) strategy resulted in the up- and down-regulation of hundreds of mRNAs, of which a disproportionately high fraction accumulates in a circadian fashion. miR-122 has previously been linked to the regulation of cholesterol and lipid metabolism. The transcripts associated w

Circadian rhythms are generated by well-conserved interlocked transcriptional feedback loops in animals. In Drosophila, the dimeric transcription factor CLOCK/CYCLE (CLK/CYC) promotes period (per), timeless (tim), vrille (vri), and PAR-domain protein 1 (Pdp1) transcription. PER and TIM negatively feed back on CLK/CYC transcriptional activity, whereas VRI and PDP1 negatively and positively regulate Clk transcription, respectively. Here, we show that the ? isoform of the Drosophila FOS homolog KAYAK (KAY) is required for normal circadian behavior. KAY-? downregulation in circadian pacemaker neurons increases period length by 1.5 h. This behavioral phenotype is correlated with decreased expression of several circadian proteins. The strongest effects are on CLK and the neuropeptide PIGMENT DISPERSING FACTOR, which are both under VRI and PDP1 control. Consistently, KAY-? can bind to VRI and inhibit its interaction with the Clk promoter. Interestingly, KAY-? can also repress CLK activity. Hence, in ...

Expression of ARNTL2 (bHLHe6, BMAL2, CLIF, MOP9, PASD9) in lymph node tissue. Antibody staining with HPA059074 in immunohistochemistry.

Transcriptional repressor which coordinates circadian rhythm and metabolic pathways in a heme-dependent manner. Integral component of the complex transcription machinery that governs circadian rhythmicity and forms a critical negative limb of the circadian clock by directly repressing the expression of core clock components ARNTL/BMAL1 and CLOCK. Also regulates genes involved in metabolic functions, including lipid metabolism and the inflammatory response. Acts as a receptor for heme which stimulates its interaction with the NCOR1/HDAC3 corepressor complex, enhancing transcriptional repression. Recognizes two classes of DNA response elements within the promoter of its target genes and can bind to DNA as either monomers or homodimers, depending on the nature of the response element. Binds as a monomer to a response element composed of the consensus half-site motif 5-[A/G]GGTCA-3 preceded by an A/T-rich 5 sequence (RevRE), or as a homodimer to a direct repeat of the core motif spaced by two

Fingerprint Dive into the research topics of Dysfunction of the circadian transcriptional factor CLOCK in mice resists chemical carcinogen-induced tumorigenesis. Together they form a unique fingerprint. ...

Their protein products act as modifiers - they may add or remove phosphate groups on core clock genes, inhibit or stimulate expression of some of the core clock genes, degrade the core clock proteins either spontaneusly or upon receiving a signal that the retinae have perceived light, etc ...

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Fat cells store excess energy and signal these levels to the brain. In a new study this week in Nature Medicine researches show that deletion of the clock gene Arntl, also known as Bmal1, in fat cells, causes mice to become obese, with a shift in the timing of when this nocturnal species normally eats. These findings shed light on the complex causes of obesity in humans.

LMU researchers have shown that circadian oscillations in the influx of immune cells into the damaged tissue play a crucial role in exacerbating the effects of an acute heart attack in the early morning hours.

The classical notion of a centralized clock that governs circadian rhythmicity has been challenged with the discovery of peripheral oscillators that enable organisms to cope with daily changes in their environment. The present study aimed to identify the molecular clock components in Atlantic cod (Gadus morhua) and to investigate their daily gene expression in fast skeletal muscle. Atlantic cod clock genes were closely related to their orthologs in teleosts and tetrapods. Synteny was conserved to varying degrees in the majority of the 18 clock genes examined. In particular, aryl hydrocarbon receptor nuclear translocator-like 2 (arntl2), RAR-related orphan receptor A (rora) and timeless (tim) displayed high degrees of conservation. Expression profiling during the early ontogenesis revealed that some transcripts were maternally transferred, namely arntl2,cryptochrome 1b and 2 (cry1b and cry2), and period 2a and 2b(per2a and per2b). Most clock genes were ubiquitously expressed in various tissues, ...

Many organisms have ≈24-h rhythms in metabolism, physiology, and behavior that are driven by cell autonomous circadian pacemakers (1). These circadian rhythms allow organisms to coordinate a myriad of physiological processes with the changing environment. In mammals, the circadian pacemaker is composed of interlocked transcription-translation feedback loops: the primary loop is composed of the basic helix-loop-helix transcription factors CLOCK and BMAL1, which drive transcription of the Period (Per1, Per2) and Cryptochrome (Cry1, Cry2) genes (1, 2). PER and CRY proteins form the negative limb of the feedback loop by inhibiting their own CLOCK:BMAL1-induced transcription; turnover of PER and CRY allows the cycle to begin anew. The interlocked loop consists of REV-ERB-α and RORα, which repress and activate the Bmal1 gene, thereby modulating its function (3, 4). Mutation or deletion of Clock (5), Bmal1 (6), Per1/2 genes (7, 8), or Cry1/2 (9, 10) genes results in behavioral arrhythmicity and ...

Circadian clocks regulate daily patterns of behavior, metabolism, and immune system function. At the core of the clock are transcriptional and translational feedback loops that generate periodic, oscillating changes in the abundance of clock components. One of these loops involves the action of the transcription factors CLOCK and BMAL1, heterodimers of which stimulate the expression of genes encoding the transcription factors Period 1 and Period 2 (PER1 and PER2). PER1 and PER2 accumulate in the cytoplasm, translocate into the nucleus, and inhibit transcription of Clock and Bmal1. The timing of transcription and translation of clock components is important for determining the clocks periodicity, and Chen et al. report that microRNAs (miRNAs) play a key role in this timing mechanism. Because the circadian clock is essential for early development, the authors used mice harboring a conditional knockout of Dicer, which encodes a critical component of the miRNA processing machinery, to assess the ...

U2OS cells harbor a circadian clock but express only a few rhythmic genes in constant conditions. We identified 3040 binding sites of the circadian regulators BMAL1, CLOCK and CRY1 in the U2OS genome. Most binding sites even in promoters do not correlate with detectable rhythmic transcript levels. Luciferase fusions reveal that the circadian clock supports robust but low amplitude transcription rhythms of representative promoters. However, rhythmic transcription of these potentially clock-controlled genes is masked by non-circadian transcription that overwrites the weaker contribution of the clock in constant conditions. Our data suggest that U2OS cells harbor an intrinsically rather weak circadian oscillator. The oscillator has the potential to regulate a large number of genes. The contribution of circadian versus non-circadian transcription is dependent on the metabolic state of the cell and may determine the apparent complexity of the circadian transcriptome. Hoffmann, Julia; Symul, Laura; Shostak,

CLOCK and BMAL1 are bHLH-PAS-containing transcription factors that bind to E-box elements and are indispensable for expression of core circadian clock components such as the Per and Cry genes. A key step in expression is the heterodimerization of CLOCK and BMAL1 and their accumulation in the nucleus …

The glucocorticoid receptor (GR) is a major drug target in inflammatory disease. However, chronic glucocorticoid (GC) treatment leads to disordered energy metabolism, including increased weight gain, adiposity, and hepatosteatosis - all programs modulated by the circadian clock. We demonstrated that while antiinflammatory GC actions were maintained irrespective of dosing time, the liver was significantly more GC sensitive during the day. Temporal segregation of GC action was underpinned by a physical interaction of GR with the circadian transcription factor REVERBa and co-binding with liver-specific hepatocyte nuclear transcription factors (HNFs) on chromatin. REVERBa promoted efficient GR recruitment to chromatin during the day, acting in part by maintaining histone acetylation, with REVERBa-dependent GC responses providing segregation of carbohydrate and lipid metabolism. Importantly, deletion of Reverba inverted circadian liver GC sensitivity and protected mice from hepatosteatosis induced by ...

Manipulating Plants Circadian Clock May Make All-Season Crops Possible http://www.sciencedaily.com/releases/2011/09/110901134643.htm...

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Author: Nakamura, Y. et al.; Genre: Journal Article; Published online: 2014-07; Title: Diurnal and circadian expression profiles of glycerolipid biosynthetic genes in Arabidopsis

This gene encodes a basic helix-loop-helix protein expressed in various tissues. The encoded protein can interact with ARNTL or compete for E-box binding sites in the promoter of PER1 and repress CLOCK/ARNTLs transactivation of PER1. This gene is believed to be involved in the control of circadian rhythm and cell differentiation. [provided by RefSeq, Feb 2014 ...

As the rhythmic expression of many genes falls out of sync in older human brains, a subset of transcripts gain rhythmicity with age.. 0 Comments. ...

Core Clock: 1506MHz, Boost Clock: 1708MHz, Memory: 6144MB 8008MHz GDDR5, Stream Processors: 1280, VR Ready, PhysX/CUDA Enabled, 2 Years Warranty.

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TY - JOUR. T1 - The relationships between clinical characteristics, alcohol and psychotropic exposure, and circadian gene expression in human postmortem samples of affective disorder and control subjects. AU - Gonzalez, Robert. AU - Bernardo, Carmina. AU - Cruz, Dianne. AU - Walss-Bass, Consuelo. AU - Thompson, Peter M.. PY - 2014/8/30. Y1 - 2014/8/30. N2 - Circadian abnormalities may be related to mood disorders. Circadian gene expression was measured in postmortem brain tissue from individuals with affective disorders and controls. Relationships between circadian gene expression, clinical characteristics, and alcohol and psychotropic medication use were noted. Further study is warranted to characterize these relationships.. AB - Circadian abnormalities may be related to mood disorders. Circadian gene expression was measured in postmortem brain tissue from individuals with affective disorders and controls. Relationships between circadian gene expression, clinical characteristics, and alcohol ...

TY - JOUR. T1 - Adrenergic regulation of clock gene expression in mouse liver. AU - Terazono, Hideyuki. AU - Mutoh, Tatsushi. AU - Yamaguchi, Shun. AU - Kobayashi, Masaki. AU - Akiyama, Masashi. AU - Udo, Rhyuta. AU - Ohdo, Shigehiro. AU - Okamura, Hitoshi. AU - Shibata, Shigenobu. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2003/5/27. Y1 - 2003/5/27. N2 - A main oscillator in the suprachiasmatic nucleus (SCN) conveys circadian information to the peripheral clock systems for the regulation of fundamental physiological functions. Although polysynaptic autonomic neural pathways between the SCN and the liver were observed in rats, whether activation of the sympathetic nervous system entrains clock gene expression in the liver has yet to be understood. To assess sympathetic innervation from the SCN to liver tissue, we investigated whether injection of adrenaline/ noradrenaline (epinephrine/norepinephrine) or sympathetic nerve stimulation could induce mPer gene expression ...

article{3062551, abstract = {Previously, we identified HISTONE MONOUBIQUITINATION1 (HUB1) as an unconventional ubiquitin E3 ligase that is not involved in protein degradation but in the histone H2B modification that is implicated in transcriptional activation in plants. HUB1-mediated regulation of gene expression played a role in periodic and inducible processes such as the cell cycle, dormancy, flowering time and defense responses. Here, we determined the effects of the hub1-1 mutation on expression of a set of diurnally induced circadian clock genes identified from a comparative microarray analysis between the hub1-1 mutant and an HUB1 over-expression line. The hub1-1 mutation reduced the amplitudes of a number of induced clock gene expression peaks, as well as the HUB1-mediated histone H2BUb and H3K4Me3 marks associated with the coding regions, suggesting a role for HUB1 in facilitating transcriptional elongation in plants. Furthermore, double mutants between hub1-1 and elongata (elo) showed ...

Circadian rhythm is an internal biological clock, which enables to sustain an approximately 24-hour rhythm in the absence of environmental cues. In mammals, the circadian clock mechanism consists of cell-autonomous transcription-translation feedback loops that drive rhythmic, 24-hour expression patterns of core clock components. The first negative feedback loop is a rhythmic transcription of period genes (PER1, PER2, and PER3) and chryptochrome genes (CRY1 and CRY2). PER and CRY proteins form a heterodimer, which acts on the CLOCK/BMAL1 heterodimer to repress its own transcription. PER and CRY proteins are phosphorylated by casein kinase epsilon (CKIepsilon), which leads to degradation and restarting of the cycle. The second loop is a positive feedback loop driven by the CLOCK/BMAL1 heterodimer, which initiates transcription of target genes containing E-box cis-regulatory enhancer sequences ...

The circadian system provides living organisms a means to adapt their internal physiology to constantly changing environmental conditions that exists on our rotating planet, Earth. Clocks in peripheral tissues are referred to as peripheral which may participate in tissue-specific functions. The first step to investigating the circadian regulation in the peripheral tissues of avians was to examine for the presence of avian orthologs of core components of the molecular clock using Quantitative real time (qRTPCR) assays. We investigated the avian spleen for daily and circadian control of core clock genes and regulation of the inflammatory response by the spleen clock. The core clock genes, bmal1, bmal2, per2, per3 and clock displayed both daily and circadian rhythms. Proinflammatory cytokines TNFα, IL-1β, IL-6 and IL-18 exhibited daily and circadian rhythmic oscillations. A differential expression of proinflammatory cytokine induction was observed in the spleen undergoing lipopolysaccharide ...

Organisms possess endogenous clocks that enable them to synchronize to diurnal environmental cycles and anticipate time of day. In several model systems, it is known that the circadian molecular clock consists of transcriptional/translational autoregulatory feedback loops in which clock proteins translocate to the nucleus and regulate clock gene transcription (Reppert and Weaver, 2002; Dunlap and Loros, 2005; Hardin, 2005). In Drosophila, the molecular oscillator is composed of three feedback loops which are interlocked by virtue of the CLK control of per and tim gene transcription. The first loop results in rhythmic PERIOD (PER) and TIMELESS (TIM) clock protein production, the second generates rhythms in the CLOCK (CLK) transcription factor and the third (Clockwork Orange) modulates the expression of several clock genes known to be regulated by the CLK transcription factor by binding to the E-box regulatory element (Glossop et al., 1999; Cyran et al., 2003; Glossop et al., 2003; Yu et al., ...

The tick-tock of your biological clock may have just gotten a little louder.. Researchers at the University of Georgia report that the number of genes under control of in living things than suspected only a few years ago. The biological clock in a much-studied model organism is dramatically higher than previously reported. The new study implies that the clock may be much more important. This new finding may help to explain why the clock is so far-reaching in its effects on the organism, said Jonathan Arnold, a professor in the UGA department of genetics and director of the research project. We found that some 25 percent of the genes in our model organism appear to be under clock control. I wasnt suspecting anything remotely like that.. The new research, just published in the Public Library of Science One, also shows how Arnolds team used a new methodology called Computing Life to yield these new discoveries about biological clocks. And this tool of systems biology was the key to showing ...

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Circadian rhythms are mechanisms that measure time on a scale of about 24 h and that adjusts our body to external environmental signals. Core circadian clock genes are defined as genes whose protein products are necessary components for the generation and regulation of circadian rhythms. Circadian proteins also regulate genes involved in either cell division or death; and a perturbation of the balance among these processes leads to cancer development and progression.. A key aspect of cancer research is identifying new regulatory pathways involved in proliferation and differentiation of cell. Disruption of circadian rhythm has recently emerged as a new potential risk factor in the development of cancer, pointing to the core gene period 2 (per2) as a tumor suppressor. However, it remains unclear how the circadian network regulates tumor suppression, nor which, if any, of its components is either the ultimate effector that influences the fate of the cell.. Initial experiments were devoted to ...

This is the first study to show that RICTOR/mTORC2 acts as a regulator for coordinated diurnal expression of clock genes in PVAT, but not in the SCN. At the whole body level, RICTOR/mTORC2 in adipose and brain tissue contributes to the diurnal regulation of blood pressure and locomotor activity. The presented data demonstrate the importance of the mTORC2 signaling pathway in the brain to adipocyte axis for daily fluctuations of physiological processes.. We and others have recently shown that mTORC2 activity controls inflammatory molecule expression using tissue-specific Rictor knockout mouse models.7,20 We showed that vascular contractility in RictoraP2KO mice is increased because of enhanced secretion of proinflammatory cytokines in PVAT.7 Ablation of Rictor strongly reduced AKTSer473 phosphorylation, resulting in impaired mTORC2 signaling.5-7 Consequently, we assign the observed changes in RictoraP2KO mice to the impaired mTORC2 downstream signaling.. In this study, we explored these findings ...

SR9011 Description:. SR9011 is a trifunctional methacrylate monomer that provides exceptional adhesion to metal substrates.. SR9011 is recommended for use in peroxide cured coatings, sealants, and PVC-based plastisols. Usage levels of 5% to 10% by weight are recommended.. SR9011 is a potent and specific synthetic REV-ERB. It also has good in vivo plasma/brain exposure. The nuclear receptors REV-ERB-α and REV-ERB-β play an integral role in regulating the expression of core clock proteins, driving rhythms in activity and metabolism. Administration of SR9011 alters circadian behavior and the circadian pattern of core clock gene expression in the hypothalami of mice. The circadian expression pattern of an array of metabolic genes in the liver, skeletal muscle and adipose tissue was also altered, resulting in increased energy expenditure. Treatment of diet-induced obese mice with SR9011 decreased obesity by reducing fat mass and markedly improved dyslipidaemia and hyperglycaemia. These results ...

Sundowning or sundown syndrome causes a sudden worsening of confusion, agitation, and aggression in patients with Alzheimers disease and dementia, which was found to be governed by the bodys internal biological clock.

Hodžić A, Ristanović M, Zorn B, Tulić C, Maver A, Novaković I, Peterlin B. PLoS One. 2013;8(3):e59220. A case-control study was performed to determine the role of circadian genes in male infertility. The study consisted of 517 cases and 444 controls, all of which were of Slavic origin and recruited from three outpatient fertility centres. A chi-square test found…

Synchronization of circadian oscillators with the outside world is achieved by the acute effects of light on the levels of one or more clock components. In mammals the PAS transcription factors Clock, NPAS2, and BMAL1 regulate gene expression as a function of the day-night cycle. Both PAS domains of NPAS2 were found to bind heme as a prosthetic group, form a gas-regulated sensor, and exert heme-status control of DNA binding in vitro. In a microarray analysis comparing overall changes in brain transcript levels between mice subjected to light pulses during the dark phase with animals maintained in darkness, we traced consistent changes in more than 200 different transcripts. Of these, 20 are associated with heme and iron biosynthesis and catabolism. A model for the pathway of induction of heme and iron homeostasis-related transcripts resulting from light pulses suggests that light signals (as stressors) induce transcription of heme oxygenase 2 (Hmox2) and cytochrome P450 oxidoreductase (Por), ...

A 2/N mode dock generator that generates bus clock signals through the use of bus clock enable signals selecting bus clock pulses that are in phase and out of phase with a core clock signal. The clock generator maintains synchronization between the bus clock signal and the core clock signal so that they are always in a predetermined phase relationship.

High-fat diet disrupts circadian gene expression in insulin and glucagon secreting cells Conference Poster 2010 Congress communication ...

Circadian rhythms are intrinsic ?24 hour cycles that regulate diverse aspects of physiology, and in turn are regulated by interactions with the external environment. Casein kinase 1 delta (CK1?, CSNK1D) is a key regulator of the clock, phosphorylating both stabilizing and destabilizing sites on the PER2 protein, in a mechanism known as the phosphoswitch. CK1? can itself be regulated by phosphorylation on its regulatory domain, but the specific sites involved, and the role this plays in control of circadian rhythms as well as other CK1-dependent processes is not well understood. Using a sensitized ...

King DP, Zhao Y, Sangoram AM, Wilsbacher LD, Tanaka M, Antoch MP, Steeves TD, Vitaterna MH, Kornhauser JM, Lowrey PL, Turek FW, Takahashi JS. Cell. 1997;89(4):641-53. Genetic mapping and sequencing in mice confirmed the identification of the mammalian gene, clock, involved in the circadian system. A comparison of the nucleotide sequence of the wildtype gene with…

Disruption of two genes that control circadian rhythms can lead to diabetes, a researcher at UT Southwestern Medical Center has found in an animal study.. Mice with defective copies of the genes, called CLOCK and BMAL1, develop abnormalities in pancreatic cells that eventually render the cells unable to release sufficient amounts of insulin.. These results indicate that disruption of the daily clock may contribute to diabetes by impairing the pancreas ability to deliver insulin, said Dr. Joseph Takahashi, an investigator with the Howard Hughes Medical Institute at UT Southwestern and co-senior author of the study, which appeared in the journal Nature. Dr. Takahashi, who recently joined UT Southwestern as chairman of neuroscience, performed the research with colleagues when he was at Northwestern University.. Circadian rhythms are cyclical patterns in biological activities, such as sleeping, eating, body temperature and hormone production.. The mammalian CLOCK gene, which Dr. Takahashi ...

TY - JOUR. T1 - Translation-independent circadian control of the cell cycle in a unicellular photosynthetic eukaryote. AU - Miyagishima, Shin Ya. AU - Fujiwara, Takayuki. AU - Sumiya, Nobuko. AU - Hirooka, Shunsuke. AU - Nakano, Akihiko. AU - Kabeya, Yukihiro. AU - Nakamura, Mami. N1 - Funding Information: We thank A. Yamashita for technical support and K. Fukaya and BSIs Research Resources Center of RIKEN for LC-MS/MS analyses. This study was supported by Grant-in-Aid for Scientific Research from Japan Society for the Promotion of Science (no. 19870033 to S.-y.M.) and by Core Research for Evolutional Science and Technology (CREST) Program of Japan Science and Technology Agency (JST) (to S.-y.M.).. PY - 2014/5/8. Y1 - 2014/5/8. N2 - Circadian rhythms of cell division have been observed in several lineages of eukaryotes, especially photosynthetic unicellular eukaryotes. However, the mechanism underlying the circadian regulation of the cell cycle and the nature of the advantage conferred remain ...

Circadian clock gene expression in in vitro cultured CD4+ T cells and PER2::Luciferase CD4+ reporter T cells. Blood was sampled from 3 nutritious younger males

The 527-02 Clock Slicer is the most flexible way to generate a CMOS output clock from a PECL input clock with zero skew. The user can easily configure the device to produce nearly any output clock that is multiplied or divided from the input clock. The part supports non-integer multiplications and divisions. A SYNC pulse indicates when the rising clock edges are aligned with zero skew. Using Phase-Locked Loop (PLL) techniques, the device accepts an input clock up to 200 MHz and produces an output clock up to 160 MHz. The 527-02 aligns rising edges on PECLIN with FBIN at a ratio determined by the reference and feedback dividers. For a PECL input and output clock with zero delay, use the 527-04. For a CMOS input and PECL output with zero delay, use the 527-03. ...

The 527-01 Clock Slicer is the most flexible way to generate an output clock from an input clock with zero skew. The user can easily configure the device to produce nearly any output clock that is multiplied or divided from the input clock. The part supports non-integer multiplications and divisions. A SYNC pulse indicates when the rising clock edges are aligned with zero skew. Using Phase-Locked Loop (PLL) techniques, the device accepts an input clock up to 200 MHz and produces an output clock up to 160 MHz. The 527-01 aligns rising edges on ICLK and FBIN at a ratio determined by the reference and feedback dividers. For configurable clocks that do not require zero delay, use the 525.. ...

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There are two types of date-time clocks:. The Hardware Clock: This clock is an independent hardware device, with its own power domain (battery, capacitor, etc), that operates when the machine is powered off, or even unplugged.. On an ISA compatible system, this clock is specified as part of the ISA standard. A control program can read or set this clock only to a whole second, but it can also detect the edges of the 1 second clock ticks, so the clock actually has virtually infinite precision.. This clock is commonly called the hardware clock, the real time clock, the RTC, the BIOS clock, and the CMOS clock. Hardware Clock, in its capitalized form, was coined for use by hwclock. The Linux kernel also refers to it as the persistent clock.. Some non-ISA systems have a few real time clocks with only one of them having its own power domain. A very low power external I2C or SPI clock chip might be used with a backup battery as the hardware clock to initialize a more functional integrated real-time ...

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells which in turn regulates the rhythmic expression of the ADCY1 gene in the ganglian cell layer (By similarity). ...

Buy Ticking Clock Loops Pack by ShahruhAudio on AudioJungle. 4 various ticking clock loops Durations: ticking clock1 0:04 ticking clock2 0:08 ticking clock3 0:08 ticking clock4 0...

The CDCM1802 clock driver distributes one pair of differential clock input to one LVPECL differential clock output pair, Y0 and Y0, and one single-ended LVCMOS output, Y1.

Rukeias paper in EBioMedicine is online. A Systems-Level Analysis Reveals Circadian Regulation of Splicing in Colorectal Cancer. El-Athman R, Fuhr L, Relógio A ...

Display a clock on your website. Choose from a wide range of Analog clock widgets, and customize clock size and colour. We provide the widest range of Analog flash clock designs, to suit any website.

Display a clock on your website. Choose from a wide range of Analog clock widgets, and customize clock size and colour. We provide the widest range of Analog flash clock designs, to suit any website.

The clock uses eight individual 8 x 8 LED arrays contained in a 3D printed enclosure that hinges in the middle. When opened up the clock has a usable resolution of 8 x 64, and when its folded onto itself the resolution becomes 16 x 32.. This variable physical resolution allows for alternate display modes. When the hardware detects that its been folded into the double-height arrangement, it goes into a so-called Big Clock mode that makes it easier to see the time from a distance. But while in single-height mode, theres more horizontal real estate for adding the current temperature or other custom data. Eventually [Alejandro] wants to use MQTT to push messages to the display, but for now it just shows his name as a placeholder.. The key to the whole project is the hinged enclosure and the reed switch used to detect what position its currently in. Beyond that, theres just an ESP32 an some clever code developed with the help of the MD_Parola library written for MAX7219 and MAX7221 LED matrix ...

This clock is made to look like a scale. (Does not measure weight.) Our clocks require one AA battery, not included. Scale measures 10˝ diameter; clock base measures 7žW x 4˝D x 9˝H.

Buy Deny Designs Iveta Abolina Geo Steps 12-inch Round Clock: Wall Clocks - ✓ FREE DELIVERY possible on eligible purchases,Low prices storewide,Easy Return,To provide you with parity advantage of goods.

As the rhythmic expression of many genes falls out of sync in older human brains, a subset of transcripts gain rhythmicity with age.. 0 Comments. ...

Atomic clocks are the most precise timekeepers we have today, with the best ones keeping time to within one second in 15 billion years.

Neophytus writes An interesting story on the BBC reports on how a new type of atomic clock is near completion that would only loose about a second in every 100 million years. Within ten years they hope to have a clock with billion year accuracy which would potentially bring advances in disease rese...

This very simple, very loud talking clock is nothing but button, making it extremely easy to find & to use. The round clock base measures 4 & 1/2 inches wide.

These are our range of clocks that will speak! These Talking Clocks will speak the time and some models may not only tell the time but also the date.

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ASUS Limited Edition Dual-GPU ROG ARES II boasts dual-Radeon HD 7970 GHz Edition GPUs and 6GB GDDR5 VRAM with a boost core clock speed of 1100MHz.

A Forex Market Hours Clock, showing live opening and closing times for the major forex trading sessions. Also see Trading Strategies based on Forex Trading Hours.

This is an ingeneous and excellent add-on that is basically a really neat total waste of time :-) When you download the library to your calculator it will put a ticking clock at the bottom of the HOME view. It displays both the date and the time (once youve set them) and uses both 12hr and 24hr time format and both US and European date format ...

Moving the clocks forward by one extra hour all year in the UK could lead to children getting more exercise every day, say researchers, reports BBC News. In the UK...