Root extracts of Holostylis reniformis (Aristolochiaceae) yielded three new natural sesquiterpenes, a sesquiterpene with an unusual carbon skeleton, 4,5-seco-guaiane (7-epi-11-hydroxychabrolidione A, 1), a nine-membered lactone with new carbon skeleton (holostylactone, 2), and a new megastigmane [(6S,7E)-6,9-dihydroxy-10-(2-hydroxy-ethoxy)-4,7-megastigmadien-3-one, 3], together with bulnesol and sitosterol-3-O-β-D-glucopyranoside. The structures of these compounds were determined by spectroscopic analyses and B3LYP/STO-3G** theoretical studies.
Read "Genetic diversity of a rare species Aristolochia contorta Bunge (Aristolochiaceae) in Primorsky Krai, Russian Journal of Genetics" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Abstract: Asarum, commonly known as wild ginger, heartleaves, or little brown jugs, is a genus belonging to the family Aristolochiaceae. Asarum exhibits a Laurasian distribution, with species in Europe, Asia, and North America. Of particular interest to this study is the genus Hexastylis. Recent studies showed that Hexastylis is rooted within Asarum and suggested to be paraphyletic, but failed to adequately examine relationships within this southeastern United States complex of species. The genus, as currently understood, consists of nine species and four varieties. Pollen was examined for all currently recognized Hexastylis taxa. Although pollen analysis showed similar surface features for most taxa, some variation in morphology became visible under increased magnification. One species, Hexastylis naniflora lacked some surface features present in the other taxa. There is currently no robust molecular analysis of relationships within the genus. Three chloroplast regions were sequenced to resolve ...
Though this species is inconspicuous and rarely encountered unless actively sought, it is included here because of three fascinating ecological stories. One story is its pollination. Most pipevines are pollinated by deceiving insects into visiting the flowers; no reward is available. Usually the flowers smell like carrion or dung to attract insects that are seeking a place to lay eggs. The story of our species is even more remarkable. It is pollinated by Ceratopogonid flies; these are the small, blood-sucking flies that pester humans and other mammals in the humid summer. The flowers of Aristolochia watsonii resemble a mouse s ear translucent funnels with fur and veins and give off a musty odor. The fly apparently expects to find a blood meal, and instead is trapped inside the flower tube overnight. During the night the flower releases pollen. The following morning the flower releases the pollen-covered fly. If the fly visits another flower it effects pollination.. All pipevines contain a ...
Some people react badly to certain foods and these reactions are broadly divided into allergies and intolerances. For allergy sufferers their body will react
Effect of Fumigation on Rotylenchulus reniformis Population Density Through Subsurface Drip Irrigation Located Every Other Furrow
Photo/image Birthwort (Aristolochia clematitis), Family: Aristolochiaceae. Location Hobro, Jylland, Danmark (Cultiv). Photographer: JC Schou, Photoid 47725
Buchwalder, K., Samain, M.-S., Sankowsky, G., Neinhuis, C., & Wanke, S. 2014. Nomenclatural updates of Aristolochia subgenus Pararistolochia (Aristolochiaceae). Australian Systematic Botany 27 (1): 48-55. doi: 10.1071/SB13042 reference page ...
You might recognize this as an amazingly large-flowered specimen of the group of vines known as Dutchmans pipes, or birthworts, genus Aristolochia, often grown in gardens, and with native species occurring in much of North America and the tropics. This giant-flowered species is ARISTOLOCHIA GRANDIFLORA, commonly called Brazilian Dutchmans Pipe or Giant Pelican Flower. Its a member of the Dutchmans Pipe or Birthwort Family, the Aristolochiaceae, native to Central and South America, but planted in the tropics and subtropics worldwide. Instead of the flowers consisting of the usual calyx and corolla, they produce a corolla-like calyx, or perianth. The perianths unusual U-shaped tubes helps the flower with its pollination. The blossoms strong fragrance entice insects, mostly flies, to enter the yellow opening, and descend the tube. In the above picture you can see that the tube constricts at its bottom, then opens into a larger chamber. This makes it harder for the pollinator to find the way ...
Flower bisexual; calyx lobes generally 5; corolla generally strongly bilateral, generally 2-lipped (upper lip generally 2-lobed, lower lip generally 3-lobed); stamens generally 4 in 2 pairs, generally included, a 5th (generally uppermost) sometimes present as a staminode; pistil 1, ovary superior, chambers generally 2, placentas axile, style 1, stigma lobes generally ...
A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence ...
A quantitative PCR procedure targeting the ?-tubulin gene determined the number of Rotylenchulus reniformis Linford & Oliveira 1940 in metagenomic DNA samples isolated from soil. Of note, this outcome was in the presence ...
Apt W Jr. 1976. Survival of reniform nematode in desiccated soils. Journal of Nematology 8: 278 (Abstract).. Apt W J, Caswell EP. 1988. Application of nematicides via drip irrigation. Annals of Applied Nematology 2: 1-10.. Ayala A, Ramirez CT. 1964. Host-range, distribution, and bibliography of the reniform nematode, Rotylenchulus reniformis, with special reference to Puerto Rico. Journal of Agriculture of University of Puerto Rico 48: 140-160.. Balasubramanian P, Ramakrishnan C. 1983. Resistance to the reniform nematode Rotylenchulus reniformis in tomato. Nematologia Mediterranea 11: 203-204.. Caswell EP, deFrank J, Apt WJ, Tang C-S. 1991. Influence of nonhost plants on population decline of Rotylenchulus reniformis. Journal of Nematology 23: 91-98.. Inserra RN, Dunn RA, McSorley R, Langdon KR, Richmer AY. 1989. Weed hosts of Rotylenchulus reniformis in ornamental nurseries of southern Florida. Nematology Circular No. 171. Florida Department of Agriculture and Consumer Services, Division of ...
Larvae of the butterfly, Battus polydamas archidamas (Papilionidae: Troidini) feed exclusively on aristolochic acid (AAs)-containing Aristolochia species (Aristolochiaceae). The distribution of sequestrated AAs in the tissues (body, integument...
Nine-membered enediyne antitumor antibiotics C-1027, neocarzinostatin (NCS), and kedarcidin (KED) possess enediyne cores to which activity-modulating peripheral moieties are attached via (R)- or (S)-vicinal diols. We have previously shown that this stereochemical difference arises from hydrolysis of epoxide precursors by epoxide hydrolases (EHs) with different regioselectivities. The inverting EHs, such as SgcF, hydrolyze an (S)-epoxide substrate to yield an (R)-diol in C-1027 biosynthesis, whereas the retaining EHs, such as NcsF2 and KedF, hydrolyze an (S)-epoxide substrate to yield an (S)-diol in NCS and KED biosynthesis. We now report the characterization of a series of EH mutants and provide a predictive model for EH regioselectivity in the biosynthesis of the nine-membered enediyne antitumor antibiotics. A W236Y mutation in SgcF increased the retaining activity toward (S)-styrene oxide by 3-fold, and a W236Y/Q237M double mutation in SgcF, mimicking NcsF2 and KedF, resulted in a 20-fold ...
Published on the web: April 12, 2005. Abstract. Tagitinin A (2), a known 3,10-epoxy-germacrolide-6,7-trans-lactone isolated from Tithonia diversifolia, was investigated by single crystal X-ray diffraction analysis. It was found to have a 1β,4α,6α,7β,8β relative configuration which differed at C(1) from the 1α-orientation originally reported in the literature which was determined by Horeaus Rule. Analysis of the 1H NMR spectrum of 2 shows the molecule to maintain its crystallographically observed twist-chair-boat (TCB) nine-membered ring conformation in acetone-d6 solution. The twist-chair-boat/skew-chair-boat type 3 conformations of saturated/unsaturated nine-membered rings within 3,10-epoxy-germacrolides can be interconverted to the skew-chair-chair (SCC) conformation by means of a C(9) ring atom flip mechanism. As a result of this conformational change, the orientation of the C(1) atom and the C(8)-oxycarbonyl moiety are transformed from diequatorial to diaxial. The reported ...
Marine sponges are a rich source of natural bioactive compounds. One of the most abundant valuable products is collagen/gelatin, and therefore, presents an interesting alternative source for pharmaceutical and biomedical applications. We have previously proposed an innovative green technology for the extraction of collagen/gelatin from marine sponges based in water acidified with carbon dioxide. In this work, we have optimized the process operating conditions towards high yields and collagen quality as well as to reduce extraction procedure duration and energy consumption. The process extraction efficiency is higher than 50%, corresponding to a yield of approximately 10% of the sponge dry mass, obtained for mild operating conditions 10 bars and 3h. The extracted material was characterized by Scanning Electron Microscopy (SEM), Rheology, Fourier Transformed Infrared Spectroscopy (FTIR), Circular Dichroism (CD), Aminoacid Analysis and SDS-PAGE. The extracts were found to be composed of highly pure ...
Collagen is a natural and abundant polymer that serves multiple functions in both invertebrates and vertebrates. As collagen is the natural scaffolding for cells, collagen-based hydrogels are regarded as ideal materials for tissue engineering applications since they can mimic the natural cellular microenvironment. Chondrosia reniformis is a marine demosponge particularly rich in collagen, characterized by the presence of labile interfibrillar crosslinks similarly to those described in the mutable collagenous tissues (MCTs) of echinoderms. As a result single fibrils can be isolated using calcium-chelating and disulphide-reducing chemicals. In the present work we firstly describe a new extraction method that directly produces a highly hydrated hydrogel with interesting self-healing properties. The materials obtained were then biochemically and rheologically characterized. Our investigation has shown that the developed extraction procedure is able to extract collagen as well as other proteins and
The wild animals most sacred to Mars were the woodpecker, the wolf, and the bear, which in the natural lore of the Romans were said always to inhabit the same foothills and woodlands.[34]. Plutarch notes that the woodpecker (picus) is sacred to Mars because "it is a courageous and spirited bird and has a beak so strong that it can overturn oaks by pecking them until it has reached the inmost part of the tree."[35] As the beak of the picus Martius contained the gods power to ward off harm, it was carried as a magic charm to prevent bee stings and leech bites.[36] The bird of Mars also guarded a woodland herb (paeonia) used for treatment of the digestive or female reproductive systems; those who sought to harvest it were advised to do so by night, lest the woodpecker jab out their eyes.[37] The picus Martius seems to have been a particular species, but authorities differ on which one: perhaps Picus viridis[38] or Dryocopus martius.[39]. The woodpecker was revered by the Latin peoples, who ...
3-Dimethylamino-2,2-dimethyl-2H-azirine (1) reacts in dimethylformamide at room temperature with the six-membered cyclic hydrazides 2,3-dihydrophthalazin-1,4-dione (2) and 1,2-dihydropyridazin-3,6-dione (15) to give the zwitterionic compounds 3 and 16, respectively (Schemes 1 and 7). The mechanism of these reactions is outlined in Scheme 1 for compound 3 (cf: also Scheme 8). The first steps are thought to be similar to the known reactions of 1 with the NH-acidic compounds saccharin and phthalimide (cf. [1]). Instead of ring expansion to the nine-membered heterocycle i (X = CONH, Scheme 8), a proton transfer followed by the loss of water gives 3 (Scheme 1 ...
Oxoid Limited has extended its range of diagnostic tests available for the detection of urinary tract infections with the addition of RapID? SS/u (Site Specific urine). Click to read more...