TY - JOUR. T1 - Requirement of argininosuccinate lyase for systemic nitric oxide production. AU - Erez, Ayelet. AU - Nagamani, Sandesh C.S.. AU - Shchelochkov, Oleg A.. AU - Premkumar, Muralidhar H.. AU - Campeau, Philippe M.. AU - Chen, Yuqing. AU - Garg, Harsha K.. AU - Li, Li. AU - Mian, Asad. AU - Bertin, Terry K.. AU - Black, Jennifer O.. AU - Zeng, Heng. AU - Tang, Yaoping. AU - Reddy, Anilkumar K.. AU - Summar, Marshall. AU - OBrien, William E.. AU - Harrison, David G.. AU - Mitch, William E.. AU - Marini, Juan C.. AU - Aschner, Judy L.. AU - Bryan, Nathan S.. AU - Lee, Brendan. PY - 2011/12/1. Y1 - 2011/12/1. N2 - Nitric oxide (NO) is crucial in diverse physiological and pathological processes. We show that a hypomorphic mouse model of argininosuccinate lyase (encoded by Asl) deficiency has a distinct phenotype of multiorgan dysfunction and NO deficiency. Loss of Asl in both humans and mice leads to reduced NO synthesis, owing to both decreased endogenous arginine synthesis and an ...

TY - JOUR. T1 - Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells. AU - Hung, Yu Hsuan. AU - Huang, Hau Lun. AU - Chen, Wei Ching. AU - Yen, Meng Chi. AU - Cho, Chien Yu. AU - Weng, Tzu Yang. AU - Wang, Chih Yang. AU - Chen, Yi Ling. AU - Chen, Li Tzong. AU - Lai, Ming Derg. PY - 2017/2. Y1 - 2017/2. N2 - Arginine is a critical amino acid in specific cancer types including hepatocellular carcinoma (HCC) and melanoma. Novel molecular mechanisms and therapeutic targets in arginine metabolism-mediated cancer formation await further identification. Our laboratory has previously demonstrated that arginine metabolic enzyme argininosuccinate lyase (ASL) promoted HCC formation in part via maintenance of cyclin A2 protein expression and arginine production for channeling to nitric oxide synthase. In this study, we investigated the mechanism by which ASL regulates cyclin A2 expression. We found that ASL interacted with cyclin A2 in HCC cells and the ...

Pathogenic variants in the argininosuccinate lyase (|i|ASL|/i|) gene have been shown to cause argininosuccinate lyase deficiency (ASLD); therefore, sequencing analysis offers advantages for prenatal testing and counseling in families afflicted with this condition. Here, we performed a genetic analysis of an ASLD patient and his family with an aim to offer available information for clinical diagnosis. The research subjects were a 23-month-old patient with a high plasma level of citrulline and his unaffected parents. Whole-exome sequencing identified potential related|i| ASL|/i| gene mutations in this trio. Enzymatic activity was detected spectrophotometrically by a coupled assay using arginase and measuring urea production. We identified a novel nonsynonymous mutation (c.206A>G, p.Lys69Arg) and a stop mutation (c.637C>T, p.Arg213∗) in|i| ASL|/i| in a Chinese Han patient with ASLD. The enzymatic activity of a p.Lys69Arg ASL construct in human embryonic kidney 293T cells was significantly

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

ID ARLY_AGRVS Reviewed; 467 AA. AC B9JTJ2; DT 28-JUL-2009, integrated into UniProtKB/Swiss-Prot. DT 24-MAR-2009, sequence version 1. DT 25-OCT-2017, entry version 54. DE RecName: Full=Argininosuccinate lyase {ECO:0000255,HAMAP-Rule:MF_00006}; DE Short=ASAL {ECO:0000255,HAMAP-Rule:MF_00006}; DE EC=4.3.2.1 {ECO:0000255,HAMAP-Rule:MF_00006}; DE AltName: Full=Arginosuccinase {ECO:0000255,HAMAP-Rule:MF_00006}; GN Name=argH {ECO:0000255,HAMAP-Rule:MF_00006}; GN OrderedLocusNames=Avi_4017; OS Agrobacterium vitis (strain S4 / ATCC BAA-846) (Rhizobium vitis OS (strain S4)). OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium. OX NCBI_TaxID=311402; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=S4 / ATCC BAA-846; RX PubMed=19251847; DOI=10.1128/JB.01779-08; RA Slater S.C., Goldman B.S., Goodner B., Setubal J.C., Farrand S.K., RA Nester E.W., Burr T.J., Banta L., Dickerman A.W., Paulsen I., RA Otten L., Suen G., ...

SWISS-MODEL Repository entry for B3ECP3 (ARLY_CHLL2), Argininosuccinate lyase. Chlorobium limicola (strain DSM 245 / NBRC 103803 / 6330)

The yankees just tied up the game. The Red Sox were winning 4-0. Then it was 4-2. Then it was 5-2, then 5-3, now 5-5. argh. argh. argh. argh. argh. Sometimes, life is terribly unfair.

You ever have one of those months? I feel like I have been at the vet every other day for the last month. New pup visit with poop samples and

So I asked Chases PCs office to send the records. I inquired as to how the records would be sent, confirmed it was with an overnight service with tracking info, and was told the records would go out the next day. When I called the next day to confirm, they informed me they did in fact send the records that morning but it was via regular mail because it would have been a $35 charge for them to FedEx them. Hubs and I were not too pleased (call us crazy, but were not fans of medical records and/or personal identifying information floating around the USPS without any method of confirming delivery). I told the office I was NOT pleased that they made the decision for us concerning the $35 charge. I couldnt fathom why they couldnt pick up the phone and call me to ask what it was I wanted to do. Had they done so, I wouldve asked them to hold the copy of the records, had hubs pick them up on his way home, and send them ourselves via UPS (for waaaaaay less than $35!). But alas, it was too late. They ...

My responds - of course, I was mad and a bit confused. To clear my thought, I started to dig lot of journals regarding the chemotherapy regiment for GTD and the GTD itself. Found out that 5% of patients that undergo the MTX-FA will start to develop resistant towards the drugs. Argh… (again I considered myself as lucky-one to be in the statistical data ...

Argh! Im a week overdue with, what is predicted to be a BIG Bubba (no.2) & going for a sweep tomorrow. I just wondered, in your collective experienc

The next day, I took Josh on an outing to the Caribbean National Forest about 45 minutes East and South of the hotel, which is on the North coast of the island. The elevation rises to nearly 3000 feet and the temperature drops, probably into the high 70s. Like most of Puerto Rico, Hurricane George hammered El Yunque rain forest in September 1998. Some sites were closed for renovation but it was incredible walking along a path cut in the dense foliage where a rabbit could hardly penetrate. According to our guide, there are no poisonous snakes in Puerto Rico, something I found comforting in such tight quarters. There are only 48 Puerto Rican parrots still in existence, few if any in the wild. The coqui frog, only found in Puerto Rico, makes a two-note chirp more like a bird than your typical croaking frog. It was a wonderful feeling to be winding my way through this forest, guided expertly by Josh, feeling as smelling this incredible habitat for plants and animals I had never met ...

Although accumulating evidence highlights the importance of p53-mediated metabolism in tumor suppression (2, 47), the mechanisms by which p53 drives dynamic nutrient status in harmony with canonical p53 functions remain poorly understood. Here, we show that p53 activates the penultimate step of de novo arginine synthesis pathway through the direct induction of the rate-limiting enzyme ASS1. Furthermore, we demonstrate that ASS1 deficiency induced anomalous Akt phosphorylation, resulting in rendering cells more susceptible to genotoxic stress.. Although we have demonstrated that p53 drives the de novo arginine synthesis pathway via ASS1 induction under genotoxic conditions, argininosuccinate lyase (ASL), which directly produces arginine from argininosuccinate, was not induced by p53 in HCT116 cells. These results suggest that ASS1 is the sole node connecting p53 to the de novo arginine synthesis pathway. Because ASS1 is a rate-limiting enzyme of the de novo arginine synthesis pathway, ASS1 ...

TY - JOUR. T1 - Targeting argininosuccinate synthetase negative melanomas using combination of arginine degrading enzyme and cisplatin. AU - Savaraj, Niramol. AU - Wu, Chunjing. AU - Li, Ying Ying. AU - Wangpaichitr, Medhi. AU - You, Min. AU - Bomalaski, John. AU - He, Wei. AU - Kuo, Macus Tien. AU - Feun, Lynn G. PY - 2015. Y1 - 2015. N2 - Loss of argininosuccinate synthetase (ASS) expression in melanoma makes these tumor cells vulnerable to arginine deprivation. Pegylated arginine deiminase (ADIPEG20) which degrades arginine to citrulline and ammonia has been used clinically and partial responses and stable disease have been noted with minimal toxicity. In order to improve the therapeutic efficacy of ADI-PEG20, we have combined ADI-PEG20 with a DNA damaging agent, cisplatin. We have shown that the combination of the two drugs together significantly improved the therapeutic efficacy when compared to ADI-PEG20 alone or cisplatin alone in 4 melanoma cell lines, regardless of their BRAF mutation. ...

context: https://springernature.github.io/scigraph/jsonld/sgcontext.json, about: [ { id: http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604, inDefinedTermSet: http://purl.org/au-research/vocabulary/anzsrc-for/2008/, name: Genetics, type: DefinedTerm }, { id: http://purl.org/au-research/vocabulary/anzsrc-for/2008/06, inDefinedTermSet: http://purl.org/au-research/vocabulary/anzsrc-for/2008/, name: Biological Sciences, type: DefinedTerm }, { inDefinedTermSet: https://www.nlm.nih.gov/mesh/, name: Arginine, type: DefinedTerm }, { inDefinedTermSet: https://www.nlm.nih.gov/mesh/, name: Argininosuccinate Lyase, type: DefinedTerm }, { inDefinedTermSet: https://www.nlm.nih.gov/mesh/, name: Argininosuccinate Synthase, type: DefinedTerm }, { inDefinedTermSet: https://www.nlm.nih.gov/mesh/, name: Bacterial Proteins, type: DefinedTerm }, { inDefinedTermSet: https://www.nlm.nih.gov/mesh/, name: Biosynthetic ...

Argininosuccinate synthetase (ASS), an integral enzyme to synthesize arginine is usually down regulated in many tumors including hepatocellular carcinoma (HCC). only occurs in SNU398 and SNU387, and not in HepG2 and Huh-1 (ASS(+)) cells, purchase EPZ-5676 and it is partly because of reduced anti-apoptotic protein X-linked inhibitor of apoptosis proteins (XIAP), myeloid leukemia cell differentiation proteins (Mcl-1) and B-cell lymphoma-2 (Bcl-2). Significantly, insufficient ASS also affects important enzymes in pyrimidine synthesis (carbamoyl-phosphate synthetase2, aspartate transcarbamylase and dihydrooratase (CAD) and thymidylate synthase (TS)) and malate dehydrogenase-1 (MDH-1) in TCA routine. ADI-PEG20 treatment reduced these enzymes and produced them more purchase EPZ-5676 susceptible to 5-FU. Transfection of ASS restored these enzymes and abolished the awareness to mixture and ADI-PEG20 treatment. General, our data claim that ASS affects multiple enzymes involved with 5-FU sensitivity. ...

ID ARLY_METST Reviewed; 465 AA. AC Q2NGN7; DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot. DT 07-FEB-2006, sequence version 1. DT 25-OCT-2017, entry version 79. DE RecName: Full=Argininosuccinate lyase {ECO:0000255,HAMAP-Rule:MF_00006}; DE Short=ASAL {ECO:0000255,HAMAP-Rule:MF_00006}; DE EC=4.3.2.1 {ECO:0000255,HAMAP-Rule:MF_00006}; DE AltName: Full=Arginosuccinase {ECO:0000255,HAMAP-Rule:MF_00006}; GN Name=argH {ECO:0000255,HAMAP-Rule:MF_00006}; GN OrderedLocusNames=Msp_0618; OS Methanosphaera stadtmanae (strain ATCC 43021 / DSM 3091 / JCM 11832 / OS MCB-3). OC Archaea; Euryarchaeota; Methanobacteria; Methanobacteriales; OC Methanobacteriaceae; Methanosphaera. OX NCBI_TaxID=339860; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC 43021 / DSM 3091 / JCM 11832 / MCB-3; RX PubMed=16385054; DOI=10.1128/JB.188.2.642-658.2006; RA Fricke W.F., Seedorf H., Henne A., Kruer M., Liesegang H., RA Hedderich R., Gottschalk G., Thauer R.K.; RT The genome sequence of ...

Proc Natl Acad Sci U S A. 2013 Dec 17;110(51):E5006-15. doi: 10.1073/pnas.1321305110. Epub 2013 Dec 2. Research Support, N.I.H., Extramural

For example, the fact that methylation-dependent silencing of argininosuccinate synthetase (ASS1) [40], a rate-limiting enzyme involved in the biosynthesis of arginine, has been implicated in therapeutic resistance in several cancer types including renal cell carcinoma, hepatocellular carcinoma, malignant melanoma, glioblastoma multiforme, and platinum-resistant epithelial. ovarian cancer suggests a role for demethylating agents in these ASS1 drug-resistant find more cancers [41]. Nevertheless, despite their current nonspecific promiscuity, epigenetic agents may act on most or all tumor types, since aberrant methylation and deacetylation patterns are a hallmark of cancer cells. In particular, several of the anticancer agents described in this review activate and upregulate p53, which itself affects multiple targets [19]. Following genotoxic stress. in response to traditional therapeutic strategies such as cisplatin, doxorubicin, 5-fluorouracil, fludarabine, mitoxantrone, etoposide, or X-ray ...

Occurs when wild type phenotype is restored in an F1 individual made by crossing two independent mutants, carrying different heteroalleles

My scalp is itching and bumpy! I usually use Philip Kingsley itchy flaky shampoo and that seems to sort it out. But its so expensive. [sad] - does

You know when the government sends you a piece of paper saying how much youre due in this-or-that benefit? What happens if the government worker subsequently enters one account digit incorrectly? This is. Another family learned theyd been having money stolen from their accounts, but applied too late for simple recovery. Argh. I sure feel…

Argh. I just cant win. Well, no update tonight. Im struggling with some twisted internal error my compiler is spitting out. Itll either be reinstall time or a PC that gets tossed out the window by the end of the evening. Ill let you know how the battle goes.. ...

Argh! First, the morning starts off when Im on my new diet/exercise program (oh, help…) and then you make me cry with the doggie video….. And now I have a combination of the slinky tune and the log tune from ren and stimpy stuck in my head….. @ Samantha, that popped in my head too! And it wont go away!!. ...

And, yes, the ultra sound showed that I had an enlarged thyroid with nodules. Could this possibly be the cause of my chronic cough that has progressively gotten worse over the past 7 or 8 years? At first it looked like the cough was solved. But gradually over the month or so since the surgery, Ive started coughing again. Not as much, but its still there. ARGH! Hope I didnt go through this for nothing ...

And, yes, the ultra sound showed that I had an enlarged thyroid with nodules. Could this possibly be the cause of my chronic cough that has progressively gotten worse over the past 7 or 8 years? At first it looked like the cough was solved. But gradually over the month or so since the surgery, Ive started coughing again. Not as much, but its still there. ARGH! Hope I didnt go through this for nothing ...

The list of chores for this weekend is mighty, so when I got home from work this afternoon I was planning to get started on that list a little early. I figured I would start with the thing I wanted to do the least, fix the peeling paint in the mudroom. I dont know what happened but for some reason the recently applied primer has decided not to stick to the wall compound on the mudroom walls, so the paint is peeling right off. Argh ...

The list of chores for this weekend is mighty, so when I got home from work this afternoon I was planning to get started on that list a little early. I figured I would start with the thing I wanted to do the least, fix the peeling paint in the mudroom. I dont know what happened but for some reason the recently applied primer has decided not to stick to the wall compound on the mudroom walls, so the paint is peeling right off. Argh ...

*snip* Argh. Whilst im not a fan of throwing the word innovation around, i feel just the same way about... authenticity or somewhat (at least in...

Broad said: Like I said, my feelings are complicated on the matter, so ... Im interested, however, in Her Highness thoughts on… ...[go].. Caterina said: ARGH!!! Not to deny you your goddess-given right of reflections and wishing what might-have-beens, but this guy was straight up… ...[go].. Wholovesya? said: By the by, guess who was most nasty about the charitable giving? The frigging church. My church and my moms… ...[go].. Wholovesya? said: By the by, Im not the only one I know. I have friends who work at soup kitchens because theyre… ...[go].. Wholovesya? said: As you know, I was a voyeur to the beginning of this, and I was loving your comment! I have… ...[go].. ...

Broad said: Like I said, my feelings are complicated on the matter, so ... Im interested, however, in Her Highness thoughts on… ...[go].. Caterina said: ARGH!!! Not to deny you your goddess-given right of reflections and wishing what might-have-beens, but this guy was straight up… ...[go].. Wholovesya? said: By the by, guess who was most nasty about the charitable giving? The frigging church. My church and my moms… ...[go].. Wholovesya? said: By the by, Im not the only one I know. I have friends who work at soup kitchens because theyre… ...[go].. Wholovesya? said: As you know, I was a voyeur to the beginning of this, and I was loving your comment! I have… ...[go].. ...

Yes you did!. No I didnt!. It was obviously you, who else would be so clumsy and careless?!. (sound of a door slamming). See Im right! Childish fool! Cant get any sense into your thick head! Argh!! Taeyeon yelled. He had been arguing with his younger brother Taejin the whole night over a trivial matter that grew out of proportion. Feeling very frustrated, he decided to get out of the house to cool himself down.. Grabbing a few items from his room, Taeyeon told his mother he would be staying over at a friends house tonight before he stormed out of the door.. ~*~*~*~. Ping. A tiny stone bounced off the window. Taeyeon waited to see if there was any movement. After two minutes, there was none besides the rustling of the leaves in the light breeze. He picked up another small stone and threw it against the window.. Ping. Nothing. Anxious, Taeyeon pulled out his mobile phone from his back pocket to type a message. He sat cross legged on the ground, with his back against the door of the ...

Maaheimo, H., Fiaux, J., Cakar, Z. P., Bailey, J. E., Sauer, U. & Szyperski, T., 2001, In : FEBS Journal. 268, 8, p. 2464-2479 16 p.. Research output: Contribution to journal › Article › Scientific › peer-review ...

Treatment may require the coordinated efforts of a team of specialists. Pediatricians, neurologists, geneticists, dieticians, and physicians who are familiar with metabolic disorders may need to work together to ensure a comprehensive approach to treatment. Occupational, speech language, and physical therapists may be needed to treat children with developmental disabilities. Genetic counseling is recommended for affected individuals and their families.. The treatment of argininosuccinic aciduria is aimed at preventing excessive ammonia from being formed or from removing excessive ammonia during a hyperammonemic episode. Long-term therapy combines dietary restrictions and the stimulation of alternative methods of converting and excreting nitrogen from the body (alternative pathways therapy).. Dietary restrictions in individuals with argininosuccinic aciduria are aimed at limiting the amount of protein intake to avoid the development of excess ammonia. However, enough protein must be taken in by ...

Argininosuccinate lyase deficiency (ASLD; also known as argininosuccinic aciduria) is the second most common urea cycle disorder (UCD) and accounts for 15-20% of all disorders of ureagenesis. Individuals with ASLD can have unique clinical and physiologic characteristics as compared to other UCDs. Previous work from the members of the UCDC have shown that in spite of having fewer episodes of hyperammonemia as compared to those with proximal blockade of the urea cycle, individuals with ASLD can develop intellectual and learning disabilities. Neurocognitive deficits have been observed even in individuals without any documented hyperammonemia. Furthermore, hepatic abnormalities including hepatomegaly, hepatic injury, fibrosis and even frank cirrhosis, and vascular issues like hypertension are well known in the disorder. Previous work from the members of the UCDC has demonstrated a tissue- and molecular-specific role for ASL in the generation of NO. ASL is not only required for the synthesis of ...

Argininosuccinic Aciduria; Arginino Succinase Deficiency. On-line free medical diagnosis assistant. Ranked list of possible diseases from either several symptoms or a full patient history. A similarity measure between symptoms and diseases is provided.

Citrullinemia type I is an autosomal recessive disorder that is caused by a deficiency of the urea cycle enzyme argininosuccinate synthetase (ASS1). Deficiency of ASS1 shows various clinical manifestations encompassing severely affected patients with fatal neonatal hyperammonemia as well as asymptomatic individuals with only a biochemical phenotype. This is a comprehensive report of all 87 mutations found to date in the ASS1 gene on chromosome 9q34.1. A large proportion of the mutations (n=27) are described here for the first time. Mutations are distributed throughout exons 3 to 15, most of them being identified in exons 5, 12, 13, and 14. The mutation G390R in exon 15 is the single most common mutation in patients with the classical phenotype. Certain mutations clearly link to specific clinical courses but the clinical phenotype cannot be anticipated in all patients. This update presents a survey of the correlation between mutations in the ASS1 gene and the respective clinical courses as ...

In the linear pathway (Figure 1A), GLU is converted to acetylglutamate (Ac-GLU) by N-acetylglutamate synthase (NAGS, encoded by argA) which is inhibited by ARG through negative feedback regulation [36],[39]. Sequential catalytic reactions catalyzed by the next three enzymes, N-acetylglutamate kinase (NAGK, encoded by argB), N-acetylglutamate semialdehyde dehydrogenase (encoded by argC) and N-acetylornithine transaminase (encoded by argD), which are common in the three pathways (Figure 1), yield N-acetylornithine (Ac-ORN) [34]. The next step, which distinguishes the linear pathway from the other two pathways, is deacetylation of Ac-ORN by AOase to yield ORN [40],[41]. The next and final steps are carried out by ornithine carbamoyltransferase (OTC or OTCase, encoded by argF), argininosuccinate synthase (encoded by argG) and argininosuccinate lyase (encoded by argH), which finally yield ARG [35]. This pathway has been found in a few species such as Myxococcus xanthus [41] and E. coli [36].. In many ...

Pancreatic cancer is a leading cause of cancer-related deaths in the world with a 5-year survival rate of less than 6%. Currently, there is no successful therapeutic strategy for advanced pancreatic cancer, and new effective strategies are urgently needed. Recently, an arginine deprivation agent, arginine deiminase, was found to inhibit the growth of some tumor cells (i.e., hepatocellular carcinoma, melanoma, and lung cancer) deficient in argininosuccinate synthetase (ASS), an enzyme used to synthesize arginine. The purpose of this study was to evaluate the therapeutic efficacy of arginine deiminase in combination with gemcitabine, the first line chemotherapeutic drug for patients with pancreatic cancer, and to identify the mechanisms associated with its anticancer effects. In this study, we first analyzed the expression levels of ASS in pancreatic cancer cell lines and tumor tissues using immunohistochemistry and RT-PCR. We further tested the effects of the combination regimen of arginine deiminase

Kleijer WJ et al. (2002) Clinical, enzymatic, and molecular genetic characterization of a biochemical variant type of argininosuccinic aciduria: prenatal and postnatal diagnosis in five unrelated families.. [^] ...

p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...

Sreekumar A, Poisson LM, Rajendiran TM, Khan AP, Cao Q, Yu J, Laxman B, Mehra R, Lonigro RJ, Li Y, Nyati MK, Ahsan A, Kalyana-Sundaram S, Han B, Cao X, Byun J, Omenn GS, Ghosh D, Pennathur S, Alexander DC, Berger A, Shuster JR, Wei JT, Varambally S, Beecher C, Chinnaiyan AM: Metabolomic profiles delineate potential role for sarcosine in prostate cancer progression. Nature. 2009 Feb 12;457(7231):910-4. Pubmed: 19212411 ...

1KH2: Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action.

TY - JOUR. T1 - Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction. AU - Cheng, Chun Ting. AU - Qi, Yue. AU - Wang, Yi Chang. AU - Chi, Kevin K.. AU - Chung, Yiyin. AU - Ouyang, Ching. AU - Chen, Yun Ru. AU - Oh, Myung Eun. AU - Sheng, Xiangpeng. AU - Tang, Yulong. AU - Liu, Yun Ru. AU - Lin, H. Helen. AU - Kuo, Ching Ying. AU - Schones, Dustin. AU - Vidal, Christina M.. AU - Chu, Jenny C.Y.. AU - Wang, Hung Jung. AU - Chen, Yu Han. AU - Miller, Kyle M.. AU - Chu, Peiguo. AU - Yen, Yun. AU - Jiang, Lei. AU - Kung, Hsing-Jien. AU - Ann, David K.. PY - 2018/12/1. Y1 - 2018/12/1. N2 - Defective arginine synthesis, due to the silencing of argininosuccinate synthase 1 (ASS1), is a common metabolic vulnerability in cancer, known as arginine auxotrophy. Understanding how arginine depletion kills arginine-auxotrophic cancer cells will facilitate the development of anti-cancer therapeutic strategies. Here we show that depletion of extracellular arginine in ...

Complete information for ASS1P12 gene (Pseudogene), Argininosuccinate Synthetase 1 Pseudogene 12, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Complete information for ASS1P14 gene (Pseudogene), Argininosuccinate Synthetase 1 Pseudogene 14, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

SWISS-MODEL Template Library (SMTL) entry for 1kh3.1. Crystal Structure of Thermus thermophilus HB8 Argininosuccinate Synthetase in complex with inhibitor

I started blogging years ago as a weight loss tool. I lost and gained a lot of weight over the years but more importantly I gained a lot of friends who still read my blog! Thru my 12 years of blogging, life has changed quite a bit for me! Im now disabled, living with my parents and one of my adult sons and Im now Nana to Cosette and Oliver, born 3 months apart. Life looks different for me now so I wanted my blog to look different. Welcome to the Nana Life. ...

David is a young hobby astronomer, who one day accidentally shots down an alien spacecraft by his self-made model rocket. In just that moment the UFO crashes in front of him his own rocket knocks him out. He awakes on a foreign farm and has to find out...

If I needed jeans, going to a store to try them on involved deep breaths, go in, remind myself Im healthy, Im happy, there is more to life than my weight, will they do? yes theyll do? Do I feel good in them? God what does that even feel like? Im never going to feel good in anything… too many rolls, too much spill over the top - do the pockets make my hips look wider? Argh I feel sick. Hand over the cash and get out of the store as fast as possible. Back to home, and back to baggy jumpers. ...

Then a few days later I want to do a show ip bgp hmm output not as expected. Right this time I twig it has to be one of those pesky VRFs getting in my way. So lets try doing show ip vrf abc wooh no bgp command. Okay lets try figure this out show ip ? and look through the options hmm nothing there so how about a show ip bgp ? hmm nothing there either. Argh so I am stumped how the heck do you do a show ip bgp when you have VRFs around? Time to prepare colleague for another stupid question by offering to make him a drink. Once drink has been made then I utilize my chance for another stupid question. Umm so you know these VRFs how do you do a show ip bgp turns out the answer is to do a show ip bgp vpnv4 vrf abc. So I have enough trouble with VRFs and then they go add the vpnv4 command before the vrf command. Guess I will get to that bit when I sort out multi-protocol BGP. Anyhow you will be pleased to know that I am getting on a lot better with VRFs now. However as with anything on this blog ...