New DNA Sequences ======================= AC013430 AC013430 88172bp DNA HTG 11-NOV-1999 Arabidopsis thaliana chromosome 1 clone F3F9, WORKING DRAFT SEQUENCE, 6 unordered pieces. HTG; HTGS_PHASE1. ATF11C1 AL132976 105644bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F11C1 ATF15G16 AL132959 104868bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F15G16 ATF18N11 AL132953 91275bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F18N11 ATF1P2 AL132955 101154bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F1P2 ATF24B22 AL132957 100285bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F24B22 ATF24M12 AL132980 129515bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F24M12 ATF2A19 AL132962 95993bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F2A19 ATF2K15 AL132956 130956bp DNA PLN 12-NOV-1999 Arabidopsis thaliana DNA chromosome 3, BAC clone F2K15 ...
Here, we report our effort in generating an ORFeome collection for the Arabidopsis transcription factor (TF) genes. In total, ORFeome clones representing 1,282 Arabidopsis TF genes have been obtained in the Gateway high throughput cloning pENTR vector, including 411 genes whose annotation lack cDNA support. All the ORFeome inserts have also been mobilized into a yeast expression destination vector, with an estimated 85% rate of expressing the respective proteins. Sequence analysis of these clones revealed that 34 of them did not match with either the reported cDNAs or current predicted open-reading-frame sequences. Among those, novel alternative splicing of TF gene transcripts is responsible for the observed differences in at least five genes. However, those alternative splicing events do not appear to be differentially regulated among distinct Arabidopsis tissues examined. Lastly, expression of those TF genes in 17 distinct Arabidopsis organ types and the cultured cells was profiled using a ...
Studies on epidermal cell fate determination have been important for gaining insight into the genetic and molecular mechanisms leading to the differentiation and patterning of cells. In Arabidopsis, the organization and development of many epidermal characters including trichomes, root hairs and the seed coat have been found to be controlled by a single combinatorial transcription factor complex consisting of a WD-repeat containing protein, Transparent Testa Glabra 1 (TTG1), and various MYB and bHLH proteins. The work here consists of identification of Glabra2 (GL2) and Transparent Testa Glabra2 (TTG2) as direct transcriptional targets of the TTG1 combinatorial complex, further characterization of GL2 function, and identification of transcriptional targets of GL2 and TTG2. Both GL2 and TTG2 are important in the regulation of trichomes, root hairs and seed coat development. vii GL2 has been identified as an important regulator of epidermal cell fate for over fifteen years yet there is little ...
The Arabidopsis pseudo-response regulator, APRR1, has a unique structural design containing a pseudo-receiver domain and a C-terminal CONSTANS motif. This protein was originally characterized as a presumed component of the His-to-Asp phosphorelay systems in Arabidopsis thaliana. Recently, it was reported that APRR1 is identical to the TOC1 gene product, a mutational lesion of which affects the periods of many circadian rhythms in Arabidopsis plants. TOC1 is believed to be a component of the presumed circadian clock (or central oscillator). Based on these facts, in this study four more genes, each encoding a member of the APRR1/TOC1 family of pseudo-response regulators were identified and characterized with special reference to circadian rhythms. It was found that all these members of the APRR1/TOC1 family (APRR1, APRR3, APRR5, APRR7, and APRR9) are subjected to a circadian rhythm at the level of transcription. Furthermore, in a given 24 h period, the APRR-mRNAs started accumulating sequentially ...
Dear Colleagues, JASPAR (http://jaspar.genereg.net/) is the open access reference database for transcription factor (TF) DNA binding profiles. Its used in major projects such as ENCODE or FANTOM or tools such as the MEME suite. Plants are underrepresented in the current Jaspar version and I am currently collecting information to include as many Arabidopsis TF as possible in the next Jaspar release. This effort will be useful for the Arabidopsis community as many tools using Jaspar will become available for Arabidopsis in the future if the database is populated with Arabidopsis TF. The TF specificity can be derived from ChIP-chip, ChIP-Seq, Selex/BSSA, Protein binding microarray. I have collected from the literature but I might have missed some factors. If you have generated such data in the past, or if such data exists for TF youre working on, it would be very helpful if you could send me a short mail indicating the TF name and the reference. You can also share unpublished ChIP or Selex data ...
Our observation that both Si1 and Zmm16 are sufficient to rescue their corresponding Arabidopsis mutants also provides compelling evidence for the conservation of B-class function. It would be expected, if B-class genes had evolved significantly different roles in either maize or Arabidopsis, that the maize genes would not be sufficient to functionally replace the Arabidopsis genes. However, our results indicate that the maize genes, either in combination with their respective Arabidopsis B-class protein partners, or together in Arabidopsis ap3 pi double mutants, are capable of correctly regulating the downstream targets necessary for stamen and petal development, even though in maize, B-class activity is essential for promoting stamen and lodicule development.. It is important to note that the rescue seen in the two strong lines examined was correlated with higher levels of expression than that of the Arabidopsis orthologs. Consequently, it may be necessary to have higher levels of the maize ...
Also known as: IN Arabidopsis, Arabidopsis., Arabidopsis At, Arabidopsis AS, Arabidopsis, Ara-bidopsis, ARABIDOPSIS In, ARABIDOPSIS ...
FUNCTIONS IN MOLECULAR_FUNCTION UNKNOWN INVOLVED IN BIOLOGICAL_PROCESS UNKNOWN EXPRESSED IN 18 PLANT STRUCTURES EXPRESSED DURING 9 GROWTH STAGES CONTAINS INTERPRO DOMAIN/S PROTEIN OF UNKNOWN FUNCTION DUF618 (INTERPROIPR006903) REGULATION OF NUCLEAR PRE-MRNA PROTEIN (INTERPROIPR006569) ENTH/VHS (INTERPROIPR008942) BEST ARABIDOPSIS THALIANA PROTEIN MATCH IS UNKNOWN PROTEIN (TAIRAT5G100601) HAS 3523 BLAST HITS TO 3241 PROTEINS IN 333 SPECIES ARCHAE - 25 BACTERIA - 251 METAZOA - 1575 FUNGI - 421 PLANTS - 142 VIRUSES - 17 OTHER EUKARYOTES - 1092 (SOURCE NCBI BLINK ...
Experimental: This means that the indicated PPI was experimentally demonstrated using Arabidopsis thaliana proteins as model of study. The experiment indicated links to the publication of which this interaction was annotated ...
Despite the importance of secondary growth in plants, relatively few genes regulating this process have been identified to date. By using data from detailed transcript profiling of the poplar wood-forming tissues, 150 genes that are differentially expressed within the zone of secondary growth were identified. In order to determine the possible function of these poplar genes, potential Arabidopsis thaliana orthologs were identified and gene knockout lines analysed. Three selection filters were used to identify the most likely orthologous genes using poplar and Arabidopsis sequence comparisons, expression profiling in secondary thickened Arabidopsis hypocotyls and global expression analysis of Arabidopsis tissues. Three genes encoding AtCSLA2 (At5g22740), the AtGUT1 GT47 glycosyltransferase (At1g27440) and a protein with no proposed function AtUNKA (At4g27435) were selected for further detailed analysis of their role in secondary growth in Arabidopsis. The presented genome-based approach using ...
... , Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
... , Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
... , Arabidopsis Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Arabidopsis Gene Expression Database. Created and developed by Huaming Chen
Plant parasitic nematodes, including root-knot nematodes and cyst nematodes, cause extensive damage and loss to many crops. Nematodes elicit a…
With the set of Arabidopsis plasma membrane phosphorylation sites presented in this work, we have made two major achievements. First, as a database resource, the data will facilitate signaling research on the proteins identified. Technically, it remains difficult, if not impossible, for most laboratories to identify phosphorylation sites within their proteins of interest. These data, and those that follow, should accelerate studies into the mechanistic regulation of biological processes in plants in a manner comparable to the way genome and EST sequencing projects have facilitated the cloning of genes of interest. All data will be released as a free access, searchable database through PlantsP (http://plantsp.sdsc.edu). Second, in a wider scope, the data provide insight into general emerging patterns of regulation through phosphorylation as well as an appreciation of the complexity of these processes.. It is important to stress that the identification of phosphorylation sites as described in this ...
In most regions and when doing a comparison of the whole genomes, Arabidopsis shows a great affinity for mononucleotide repeats compared to rice (Figures 1 to 5). The comparison between the whole genomes of rice and Arabidopsis clearly shows that Arabidopsis has a higher percentage of mononucleotide repeats (33.9%) than rice (18.3%). The only regions where mononucleotide repeats are not as high are the 5UTR and coding regions. Rice seems to have a greater affinity for trinucleotide repeats, with an exceptionally high density of approximately 1,670/MB in the 5UTR, even higher than in exon regions (approximately 421.4/MB). In fact, trinucleotide SSRs are the major type of SSR in all regions except 3UTRs (Figures 1 to 5).. Yet despite these differences in which type of SSR is most common for each organism, rice and Arabidopsis show similar distribution of the SSR types (period) in their coding regions. Both have a majority of trinucleotide repeats (Arabidopsis 65.4%, rice 64%) and a lack of any ...
Gene expression databases for Arabidopsis and other organisms are important resources to investigate the expression pattern of a given gene or gene families, to identify genes that respond to specific stimuli, and to search for coexpressed genes (http://www.ncbi.nlm.nih.gov/geo/; http://www.ebi.ac.uk/arrayexpress/; Steinhauser et al., 2004; Zimmermann et al., 2004; Shen et al., 2005). AtGenExpress is a very recent gene expression database for Arabidopsis, and possibly the most standardized and comprehensive gene expression resource for any multicellular organism (Altmann et al., 2004). We complemented this dataset with our own data (see introduction) and used the resulting set of Affymetrix Arabidopsis ATH1 whole-genome GeneChip data (approximately 16 million data points i.e. 721 arrays or 323 conditions) to investigate the expression stability of traditional reference genes and to identify novel Arabidopsis reference genes (see Supplemental Table I) that outperform the traditional ones in terms ...
According to the prediction of the involvement of bZIP transcription factors in the ER stress response, AtbZIP60 was identified by genome-wide screening based on genomic information on Arabidopsis. Tunicamycin and other reagents activating the ER stress response induced transcripts of AtbZIP60. From these results, we predicted that AtbZIP60 plays a role in the ER stress response. Because the expression profile of AtbZIP60 was close to that of BiP, induction of AtbZIP60 transcript was not considered to be the first trigger of activation for BiP expression. Instead, it was assumed that a conformational change of AtbZIP60 activates the expression of chaperone genes, such as BiP. This prediction was based on the fact that AtbZIP60 contains a putative TMD like that of ATF6 in mammalian cells. Specifically, it was hypothesized that AtbZIP60 is converted to a soluble form by ER stress and becomes localized to the nucleus, resulting in the activation of chaperone genes. Indeed, a truncated form of ...
Transmembrane leucine-rich repeat (LRR) receptors are commonly used innate immune receptors in plants and animals but can also sense endogenous signals to regulate development. BAK1 is a plant LRR-receptor-like kinase (RLK) that interacts with several ligand-binding LRR-RLKs to positively regulate their functions. BAK1 is involved in brassinosteroid-dependent growth and development, innate immunity, and cell-death control by interacting with the brassinosteroid receptor BRI1, immune receptors, such as FLS2 and EFR, and the small receptor kinase BIR1, respectively.. ...
Cyclome: Arabidopsis Cyclome Functional Genomics Database. gebd Arabidopsis Genome Browser. iSect Tools, iView Tools and Gene Expression Atlas. Collection of Arabidopsis T-DNA/Ds, Full-length cDNA, Marker, EST, MPSS, SAGE, miRNA, sRNA, Arabidopsis Tiling Array and Gene Expression Data. Created and developed by Huaming Chen
Arabidopsis PR1 is a salicylic acid (SA) inducible marker gene for systemic acquired resistance (SAR). However, the regulation of PR1 in plants is poorly understood. In this study, we showed that AtWRKY50 transcription factor binds to two promoter elements of PR1 via its DNA binding domain. Interestingly, the DNA-binding sites for AtWRKY50 deviate significantly from the consensus WRKY binding W-box. The binding sites are located in close proximity to the binding sites for TGA transcription factors. Transactivation experiments in Arabidopsis protoplasts derived from wild type, npr1-1 and tga256 mutant plants indicated that AtWRKY50 alone was able to induce expression of a PR1::β-glucuronidase (GUS) reporter gene, independent of TGAs or NPR1. However, co-expression of TGA2 or TGA5 with AtWRKY50 synergistically enhanced expression to high levels. Yeast-2-hybrid assays and bimolecular fluorescence complementation (BiFC) experiments revealed that AtWRKY50 could interact with TGA2 and TGA5. Using ...
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Cops7a (untagged) - Mouse COP9 (constitutive photomorphogenic) homolog, subunit 7a (Arabidopsis thaliana) (Cops7a), transcript variant 1, (10ug), 10 µg.
Scientific Experts, Research Topics, Locale, Genomes and Genes, Species, Publications about Experts and Doctors on arabidopsis proteins in Versailles, Île de France, France
The Arabidopsis thaliana genome contains genes encoding 10 MAP kinase kinase (MKK) and 20 Map kinase (MAPK) proteins, however the exact relationship between upstream MKK activators and downstream MAPK protein partners has not fully elucidated. In this study, the yeast two -hybrid system was used to identify protein-protein interactions between all Arabidopsis MAPKs and MKKs. In the yeast two- hybrid assay, the result from the qualitative β-galactosidase filter assay showed that 6 MAPK proteins interacted with 6 MKK proteins. Quantitative β-galactosidase liquid assay was used to confirm the interactions between MAPK and MKK in yeast. The BiFC approach was used to test whether MKK1, MKK2 and MKK6 associated with MPK4 and MPK11 in vivo in Arabidopsis. In these assays, GFP fluorescence was observed in the protoplasts suggesting that MKK1, MKK2 and MKK6 interact with MPK4 and MPK11, respectively. However, evidence of reciprocal interaction was only seen with the combination of MKK1 and MPK11, and ...
The members of the Arabidopsis genome sequencing consortia use different tools to perform automated genome annotations and determine similarities to ESTs and known protein sequences to refine gene models. This procedure has generated a large quantity of information on the Arabidopsis genome. However, the extraction of clear biological information for a particular process from these public databases is not always easy (for instance, the word "cyclin" as a query in the Martiensried Institute for Protein Sequences database returned 37 hits, with 23 putative cyclin or cyclin-like hits). To solve this problem, we designed a protocol focused mainly on high-quality, homology-based annotation. We used a combination of two selected high-quality Arabidopsis prediction tools (Pavy et al., 1999; Schiex et al., 2001; C. Mathé and P. Rouzé, personal communication) together with pure experimental information as reference material. One advantage of this method is that the chance of finding new and rarely ...
Leaf surface stomata from Arabidopsis sp, scanning electron micrograph (SEM). Stomata (plural of stoma) are tiny openings in the epidermis of a plant that are surrounded by pairs of crescent-shaped cells, called guard cells, that facilitate gas exchange. Arabidopsis is indigenous to Europe and is a N.W. American weed used in many botanical genetic studies due to its short life cycle. Arabidopsis thaliana has gained interest from the scientific community as a model organism for research on numerous aspects of plant biology. Magnification: x240 when shortest axis printed at 25 millimetres. - Stock Image C037/0540
Genetic redundancy of homologous genes is thought to be due to gene duplication events during the evolution of the organism. Between homologous genes, genetic redundancy can be classified as full redundancy, partial redundancy, and unequal redundancy [16]. While full redundancy and partial redundancy have been documented in numerous cases, unequal genetic redundancy has just begun to be recognized as a common phenomenon of genetic relationship of homologous genes [16]. Unlike non-plant organisms whose genomes contain only a single RACK1 gene, some plant genomes contain more than one RACK1 genes (Figure 1). In particular, the Arabidopsis genome contains three RACK1 genes, which share the similar gene structure with two exons and one intron, and encode three highly similar proteins with approximately 90% identity at the amino acid level [15]. However, the relationship between three Arabidopsis RACK1 homologous genes has been unknown. Previously, we showed that loss-of-function mutation in one ...
Author: Skirycz, A. et al.; Genre: Journal Article; Published in Print: 2008; Keywords: cell cycle|br/|dof transcription factor|br/|arabidopsis|br/|obp1|br/|d-type cyclin|br/|genome-wide analysis|br/|retinoblastoma-related protein|br/|dependent kinase complex|br/|gene-expression|br/|domain proteins|br/|suspension-cultures|br/|salicylic-acid|br/|tobacco|br/|growth|br/|division; Title: The DOF transcription factor OBP1 is involved in cell cycle regulation in Arabidopsis thaliana
Background Entry into mitosis is regulated by cyclin dependent kinases that in turn are phosphoregulated. In most eukaryotes, phosphoregulation is through WEE1 kinase and CDC25 phosphatase. In higher plants a homologous CDC25 gene is unconfirmed and hence the mitotic inducer Schizosaccharomyces pombe (Sp) cdc25 has been used as a tool in transgenic plants to probe cell cycle function. Expression of Spcdc25 in tobacco BY-2 cells accelerates entry into mitosis and depletes cytokinins; in whole plants it stimulates lateral root production. Here we show, for the first time, that alterations to cytokinin and ethylene signaling explain the rooting phenotype elicited by Spcdc25 expression in Arabidopsis. Results Expressing Spcdc25 in Arabidopsis results in increased formation of lateral and adventitious roots, a reduction of primary root width and more isodiametric cells in the root apical meristem (RAM) compared with wild type. Furthermore it stimulates root morphogenesis from hypocotyls when cultured ...
Chilling stress affects plant growth and productivity. However, the multi-underlying mechanisms of chilling tolerance are not well understood. Arabidopsis PCaP2 is involved in regulating the dynamic of microtubules and F-actin and Ca2+-binding ability. Here, the results showed that the PCaP2 expression was highly induced in roots, cotyledons, true leaves, lateral roots and flowers under cold stress. Compared with the wild type, PCaP2-overexpressing plants displayed the enhanced tolerance, whereas its RNAi and mutant were more sensitive in seed germination, seedling and reproductive growth under chilling stress in Arabidopsis. In addition, PCaP2 was also a positive regulator of ABA signaling pathway by analyzing the expression of PCaP2 and the phenotypes of PCaP2-overexpressing, mutant and RNAi plants under ABA treatment. Interestingly, disruption of PCaP2 inhibited the expression of CBF1, -3 and CBF-target COR genes, while increased the CBF2 expression in response to cold or ABA. Moreover, we found that
Arabidopsis annotated genes are represented by 7x7 pixel squares. The position of every gene reflects its position on the Arabidopsis chromosomes. Flower specific genes are represented in red, silique specific genes in purple, leaf specific genes in green, root specific genes in blue , callus specific genes in white, all other genes are dark grey squares. The gene squares are clickable and linked to the corresponding files in the MPSS Arabidopsis database ...
DEETIOLATED1 (DET1) plays a critical role in developmental and environmental responses in many plants. To date, the functions of OsDET1 in rice (Oryza sativa) have been largely unknown. OsDET1 is an ortholog of Arabidopsis (Arabidopsis thaliana) DET1 Here, we found that OsDET1 is essential for maint …
As a cell cycle regulator, the Myb-related CDC5 protein was reported to be essential for the G2 phase of the cell cycle in yeast and animals, but little is known about its function in plants. Here we report the functional characterization of the CDC5 gene in Arabidopsis thaliana. Arabidopsis CDC5 (A …
In several cases, the homologous WDR proteins are highly conserved throughout the length of the proteins, and appear to operate in highly analogous mechanisms, with specificity in function conferred by changes in upstream signaling pathways and/or downstream effectors. One case is AGB1, the only clear Arabidopsis ortholog of Gβ [31] (Fig. 1). Loss of AGB1 function leads to developmental pleiotropy including shortened fruits [32] and changes in patterns of cell division in the hypocotyl and root [33]. These phenotypes are associated with the derepression of genes that are normally turned on by auxin, suggesting a role for AGB1 as a negative regulator of auxin signaling [33]. There appears to be one Gα-like protein (GPA1) and two Gγ-like proteins (AGG1 and AGG2) in the Arabidopsis proteome [31], and molecular modeling and yeast two-hybrid studies of potential interactions among AGB1, GPA1 and AGG1 are not inconsistent with the possibility that these could form a heterotrimeric protein [33, 34]. ...
Salinity is a major abiotic stress in agriculture. Here, we report that SODIUM POTASSIUM ROOT DEFECTIVE3 (NaKR3), which encodes a heavy metal-associated domain protein, is involved in salt tolerance in Arabidopsis. The results of quantitative reverse transcription-polymerase chain reaction analysis revealed that NaKR3 was induced by high salinity and osmotic stresses, but not by Cu2+ stress. Transient expression of NaKR3-GFP in Arabidopsis protoplasts showed that the NaKR3 protein was localized in the cytosol.
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O-Glycosyl hydrolases family 17 protein; FUNCTIONS IN: cation binding, hydrolase activity, hydrolyzing O-glycosyl compounds, catalytic activity; INVOLVED IN: carbohydrate metabolic process; LOCATED IN: endomembrane system; CONTAINS InterPro DOMAIN/s: X8 (InterPro:IPR012946), Glycoside hydrolase, family 17 (InterPro:IPR000490), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: O-Glycosyl hydrolases family 17 protein (TAIR:AT3G55430.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink ...
Cable lugs and tools Addition catalogue 2015/2016 Lets connect. Connectivity and electrical installation Cable lugs and tools Contents Cable lugs and tools Addition catalogue 6a A Cable lugs and insulated connectors B Crimping tools Appendix 1541050000 - 2015/2016 Index X Search according to type or order number I II 1541050000 - 2015/2016 Cable lugs and insulated connectors Contents Cable lugs and insulated connectors Cable lugs and insulated connectors 1541050000 - 2015/2016 Technical data / general notes A.2 Tubular cable lugs, copper, euro-series A.5 Tubular cable lugs with angle, copper, euro-series A.8 Joint connector, copper, euro-series A.11 Crimp cable lugs, copper, DIN 46235 A.13 Tubular compression connectors, copper, DIN 46267 part 1 A.15 Terminals ring and fork-type, noninsulated A.17 Pin cable lugs DIN 46230, noninsulated A.20 Parallel connectors and joint connector, noninsulated A.21 Terminals ring type A.23 Terminals fork type A.24 Pin cable lugs / joint connector A.25 Parallel ...
Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca(2+) signaling may play a central role in this process. Free Ca(2+) dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, and mitochondrial matrix. The mechanistic basis of compartment-specific Ca(2+) dynamics is poorly understood. Here, we studied the function of At-MICU, an EF-hand protein of Arabidopsis thaliana with homology to constituents of the mitochondrial Ca(2+) uniporter machinery in mammals. MICU binds Ca(2+) and localizes to the mitochondria in Arabidopsis. In vivo imaging of roots expressing a genetically encoded Ca(2+) sensor in the mitochondrial matrix revealed that lack of MICU increased resting concentrations of free Ca(2+) in the matrix. Furthermore, Ca(2+) elevations triggered by auxin and extracellular ATP occurred more rapidly and reached higher maximal concentrations in the mitochondria of micu mutants, whereas cytosolic ...
Arabidopsis ATHB-8 protein: from Arabidopsis thaliana; contains a conserved DNA-binding domain characteristic of the HD-Zip family of transcription factors; amino acid sequence given in first source; GenBank Z50851
b)The manuscripts listed below should be referred in the publication on scientific journal.(1)Ito T, Motohashi R, Kuromori T, Mizukado S, Sakurai T, Kanahara H, Seki M and Shinozaki K (2002) A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol. 129: 1695-1699.(2)Kuromori T, Hirayama T, Kiyosue Y, Takabe H, Mizukado S, Sakurai T, Akiyama K, Kamiya A, Ito T and Shinozaki K (2004) A collection of 11800 single-copy Ds transposon insertion lines in Arabidopsis. Plant J. 37, 897-905 ...
Utility of WGAs for genomic analysis. We used a strategy for mapping transcription units that included both a traditional cDNA cloning and DNA sequencing approach and a novel high-throughput approach that utilizes tiling array technology (12, 13, 25). Sequencing ESTs and fl-cDNAs verified 40% of the annotated genes, dramatically improved genome annotation, and allowed the construction of 30% of the ORFeome. As with other large-scale cDNA-based gene collection projects (8, 15, 18), the traditional approach of sequencing fl-cDNAs reaches a point of diminishing returns: two-thirds of the total annotated Arabidopsis genes still have no corresponding fl-cDNA. The WGA technology allowed us to identify transcripts for the ANE genes and enabled construction of the remaining 30% of the Arabidopsis ORFeome. We were able to detect ∼60% of the total annotated genes (AE and ANE) using only a limited number of RNA samples (four). A more comprehensive survey of tissues and cell types should enable a more ...
The primary reason for producing these files was their easy utilization in our genomics data analysis program, PyMood ( http://allometra.com ) , for sophisticated querying of BLAST output data. When utilizing this file in PyMood as a query or one of target datasets it is easy to query for genes that have expressed or not expressed homologs in Arabidopsis. For example, a search in PyMood through BLAST output data (when any of these files are used as one of the target databases) with a query C0000 will retrieve the genes that have Arabidopsis homologs expressed in callus only ...
Understanding how dynamic molecular networks affect whole-organism physiology, analogous to mapping genotype to phenotype, remains a key challenge in biology. Quantitative models that represent processes at multiple scales and link understanding from several research domains can help to tackle this problem. Such integrated models are more common in crop science and ecophysiology than in the research communities that elucidate molecular networks. Several laboratories have modeled particular aspects of growth in Arabidopsis thaliana, but it was unclear whether these existing models could productively be combined. We test this approach by constructing a multiscale model of Arabidopsis rosette growth. Four existing models were integrated with minimal parameter modification (leaf water content and one flowering parameter used measured data). The resulting framework model links genetic regulation and biochemical dynamics to events at the organ and whole-plant levels, helping to understand the combined ...
Arabidopsis Dof3 protein: a member of the Dof protein family; from Arabidopsis thaliana; partial amino acid sequence given in first source; GenBank X79935
The reports of dual-targeted proteins in plants have steadily increased over the past years. The vast majority of these proteins are soluble proteins distributed between compartments of the non-secretory pathway, predominantly chloroplasts and mitochondria. In contrast, dual-targeted transmembrane proteins, especially of the secretory pathway, are rare and the mechanisms leading to their differential targeting remain largely unknown. Here, we report dual-targeting of the Arabidopsis DUF679 Membrane Protein 1 (DMP1) to the tonoplast (TP) and the plasma membrane (PM). In Arabidopsis and tobacco two equally abundant DMP1 isoforms are synthesized by alternative translation initiation: a full length protein, DMP1.1, and a truncated one, DMP1.2, which lacks the N-terminal 19 amino acids including a TP-targeting dileucine motif. Accumulation of DMP1.1 and DMP1.2 in the TP and the PM, respectively, is Brefeldin A-sensitive, indicating transit via the Golgi. However, DMP1.2 interacts with DMP1.1, leading to
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Over the years, Percival Scientific has designed chambers for a large number of applications. Steady demand for the Arabidopisis Series of chambers has allowed to engineer a chamber for the optimum growth of the Arabidopsis plant.. The Arabidopsis Series features:. ...
Effects of H2S on the auxin level of Arabidopsis root monitored by DR5::GUS.(a) Histochemical GUS staining patterns of DR5::GUS in 5-d-old seedlings treated wit