There is a worldwide increasing concern over the neurological risks of thimerosal (ethylmercury thiosalicylate) which is an organic mercury compound that is commonly used as an antimicrobial preservative. In this study, we show that thimerosal, at nanomolar concentrations, induces neuronal cell death through the mitochondrial pathway. Thimerosal, in a concentration- and time-dependent manner, decreased cell viability as assessed by calcein-ethidium staining and caused apoptosis detected by Hoechst 33258 dye. Thimerosal-induced apoptosis was associated with depolarization of mitochondrial membrane, generation of reactive oxygen species, and release of cytochrome c and apoptosis-inducing factor (AIF) from mitochondria to cytosol. Although thimerosal did not affect cellular expression of Bax at the protein level, we observed translocation of Bax from cytosol to mitochondria. Finally, caspase-9 and caspase-3 were activated in the absence of caspase-8 activation. Our data suggest that thimerosal ...

Myocyte death occurs in many inherited and acquired cardiomyopathies, including arrhythmogenic cardiomyopathy (ACM), a genetic heart disease plagued by the prevalence of sudden cardiac death. Individuals with ACM and harboring pathogenic desmosomal variants, such as desmoglein-2 (DSG2), often show myocyte necrosis with progression to exercise-associated heart failure. Here, we showed that homozygous Dsg2 mutant mice (Dsg2mut/mut), a model of ACM, die prematurely during swimming and display myocardial dysfunction and necrosis. We detected calcium (Ca2+) overload in Dsg2mut/mut hearts, which induced calpain-1 (CAPN1) activation, association of CAPN1 with mitochondria, and CAPN1-induced cleavage of mitochondrial-bound apoptosis-inducing factor (AIF). Cleaved AIF translocated to the myocyte nucleus triggering large-scale DNA fragmentation and cell death, an effect potentiated by mitochondrial-driven AIF oxidation. Posttranslational oxidation of AIF cysteine residues was due, in part, to a depleted ...

VARECHA, Miroslav et al. Knockdown of apoptosis-inducing factor disrupts function of respiratory complex I. Biocell [online]. 2012, vol.36, n.3, pp.121-126. ISSN 0327-9545.. Recent findings suggest that apoptotic protein apoptosis-inducing factor (AIF) may also play an important non-apoptotic function inside mitochondria. AIF was proposed to be an important component of respiratory chain complex I that is the major producer of superoxide radical. The possible role of AIF is still controversial. Superoxide production could be used as a valuable measure of complex I function, because the majority of superoxide is produced there. Therefore, we employed superoxide-specific mitochondrial fluorescence dye for detection of superoxide production. We studied an impact of AIF knockdown on function of mitochondrial complex I by analyzing superoxide production in selected cell lines. Our results show that tumoral telomerase-positive (TP) AIF knockdown cell lines display significant increase in superoxide ...

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Synthetic Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) Peptide. Species: Mammalian. Source: Synthetic. Order product ABIN938567.

Different cell-death mechanisms control many physiological and pathological processes in humans. Mitochondria play important roles in cell death through the release of pro-apoptotic factors such as cytochrome c and apoptosis-inducing factor (AIF), which activate caspase-dependent and caspase-independent cell death, respectively. Poly(ADP-ribose) polymerase 1 (PARP-1) is emerging as an important activator of caspase-independent cell death. PARP-1 generates the majority of long, branched poly(ADP-ribose) (PAR) polymers following DNA damage. Overactivation of PARP-1 initiates a nuclear signal that propagates to mitochondria and triggers the release of AIF. AIF then shuttles from mitochondria to the nucleus and induces peripheral chromatin condensation, large-scale fragmentation of DNA and, ultimately, cytotoxicity. Identification of the pro-death and pro-survival signals in the PARP-1-mediated cell-death program might provide novel therapeutic targets in human diseases.

1）Xin Li#, Guizhong Zhang#, Qian Chen, Yingxue Lin, Junxin Li, Qinggguo Ruan, Youhai Chen, Guang Yu, and Xiaochun Wan*. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor. Journal of Experimental& Clinical Cancer Research, 2016, 35:117; IF=4.357；. （2）Lulu Cui#, Jiacheng Bi#, Dehong Yan, Xiufeng Ye, Mingxing Zheng, Guang Yu, Xiaochun Wan*. JSI-124 inhibits IgE production in an IgE B cell line. BBRC(Biochemical and Biophysical Research Communications). 2017.01.29, 483(1):669-673, IF=2.466；. （3）Jiacheng Bi#, Lulu Cui#, Gang Yu, Xiaolu Yang, Youhai Chen, Xiaochun Wan*. NK cells alleviate lung inflammation by negatively regulating group 2 innate lymphoid cells. The Journal of Immunology. 2017.04.15, 198(8):3336- 3344. IF= 4.856；. （4）Hongling Zhang#, Jiacheng Bi#, Huqiang Yi, Tingting Fan, Qingguo Ruan, Lintao Cai, Youhai H Chen, Xiaochun Wan*. Silencing c-Rel in macrophages dampens Th1 and Th17 immune responses and alleviates experimental autoimmune ...

Sigma-Aldrich offers abstracts and full-text articles by [Mei Yang, Jialing Zhong, Mei Zhao, Jia Wang, Yuyu Gu, Xinghua Yuan, Jianli Sang, Changzhi Huang].

My interests center on development of instrumentation for analyzing and synthesizing proteins and genes (e.g., protein, sequenator, DNA synthesizer, DNA sequenator, protein synthesizer), molecular immunology and autoimmunity, genomics, specifically, large-scale DNA sequence analysis of immune receptor gene families, molecular evolution, cancer biology, and HIV genome analysis.. ...

All of us at SDSC look forward to working with Dr. Subramaniam in his new role as a Distinguished Scientist, said Michael L. Norman, SDSCs interim director. As a true pioneer in bioinformatics and systems biology, Dr. Subramaniam is uniquely qualified to identify new opportunities and propose innovative solutions as SDSC broadens its expertise in these exciting areas of scientific research.. Bioinformatics is the application of statistics and computer sciences to the field of molecular biology. It has been used widely in genomics and genetics, notably in research involving large-scale DNA sequencing.. I am honored to be appointed as an SDSC Distinguished Scientist, said Subramaniam. The new developments at SDSC, notably with its next generation of high-performance computing and data systems, are extraordinarily synergistic with my research plans, and I look forward to extremely valuable and productive collaborations.. In 2008, Subramaniam was awarded the Faculty Excellence in Research ...

Zhu C, Wang X, Deinum J, Huang Z, Gao J, Modjtahedi N, Neagu MR, Nilsson M, Eriksson PS, Hagberg H, Luban J, Kroemer G, Blomgren K. Cyclophilin A participates in the nuclear translocation of apoptosis-inducing factor in neurons after cerebral hypoxia-ischemia. J Exp Med. 2007 Aug 6; 204(8):1741-8 ...

HYD1 is a D-amino acid peptide that was previously shown to inhibit adhesion of prostate cancer cells to the extracellular matrix. In this study, we show that in addition to inhibiting adhesion of multiple myeloma (MM) cells to fibronectin, HYD1 induces cell death in MM cells as a single agent. HYD1-induced cell death was necrotic in nature as shown by: (a) decrease in mitochondrial membrane potential (Δψm), (b) loss of total cellular ATP, and (c) increase in reactive oxygen species (ROS) production. Moreover, HYD1 treatment does not result in apoptotic cell death because it did not trigger the activation of caspases or the release of apoptosis-inducing factor and endonuclease G from the mitochondria, nor did it induce double-stranded DNA breaks. HYD1 did initiate autophagy in cells; however, autophagy was found to be an adaptive response contributing to cell survival rather than the cause of cell death. We were further able to show that N-acetyl-L-cysteine, a thiol-containing free radical ...

The replication initiation factor Cdc6 is cleaved during apoptosis, and expression of a cleavage product is sufficient to induce apoptosis in otherwise unstressed cells. On page 77, Yim et al. report that the cleavage products can act as dominant-negative inhibitors of replication and amplify pro-death signals.. In addition to the previously identified cleavage product, Yim et al. identified a second Cdc6 fragment produced by caspase-3. Both Cdc6 fragments were sufficient to induce cell death without additional pro-apoptotic signals. Moreover, in cells exposed to apoptosis-inducing factors, ectopic expression of the Cdc6 peptides increased the rate of cell death. In contrast, expression of noncleavable Cdc6 suppressed apoptosis, indicating that fragmentation of the protein plays a causal role in the process even in the presence of known triggers.. Truncated Cdc6 interferes with loading of Mcm2 on the chromatin and thus disrupts assembly of the prereplication complex on chromosomes. That in turn ...

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Previous investigations suggest that DL-3-n-butylphthalide (NBP) is a promising multifaceted drug for the treatment of stroke. It is not clear whether NBP can treat traumatic brain injury (TBI) and what could be the mechanisms of therapeutic benefits. To address these issues, TBI was induced by a controlled cortical impact in adult male mice. NBP (100 mg/kg) or saline was intraperitoneally administered within 5 min after TBI. One day after TBI, apoptotic events including caspase-3/9 activation, cytochrome c release from the mitochondria, and apoptosis-inducing factor (AIF) translocation into the nucleus in the pericontusion region were attenuated in NBP-treated mice compared to TBI-saline controls. In the assessment of the nuclear factor kappa-light-chain-enhancer of activated B (NF-κB) pathway, NBP ameliorated the p65 expression and the p-IκB-α/IκB-α ratio, indicating reduced NF-κB activation. Consistently, NBP reduced the upregulation of proinflammatory cytokines such as tumor ...

The Ingvarsson research group is focused on understanding what factors govern the distribution of genetic variation across plant genomes, and how this drives phenotypic variation in traits that are of adaptive or economic importance. We mainly rely on computational analyses of large-scale DNA sequencing data sets but also use data on gene expression and traditional genetic mapping in combination with field and greenhouse experiments. We are especially interested in understanding the genetic architecture of local adaptation in phenology and the relative importance of genetic drift, recombination and natural selection in driving genome-wide patterns of genetic diversity.. The Ingvarsson research group is located at the Department of Plant Biology, Swedish University of Agricultural Sciences in Uppsala, Sweden, although a few people remain at the Department of Ecology and Environmental Sciences, Umeå University where we were located from 2002 to 2016.. ...

Leroy E. Hood, will receive the 2017 NAS Award for Chemistry in Service to Society. Hood is a biotech visionary who has revolutionized biology and medicine in a career that spans five decades. Among his many accomplishments, Hood invented, commercialized, and developed multiple chemical tools that address biological complexity, including the automated DNA sequencer which spearheaded the Human Genome Project.. Hoods earlier research work at Caltech led to the development of four DNA and protein sequencers and synthesizers, all of which became core instruments for contemporary molecular biology. Later, Hoods lab developed the ink-jet oligonucleotide synthesizer, a core technology for DNA chip synthesis and large-scale DNA synthesis, and the first instrument capable of global single-molecule analysis of DNA and RNA molecules.. Beyond these innovations, Hood shepherded a cross-disciplinary approach to chemistry and biology, which led to the establishment of the Science and Technology Center for ...

1. Lipid-induced cell death - Investigation of the mechanism leading to lipotoxicity. 2. Meiotic Processes within the regulation of aging - characterization of the mechanism leading to longevity in yeast and mammalian cells. 3. Autophagy (self-eating) in dying cells - Interconnection of apoptosis and autophagy via a new Bcl-2 family-protein. 4. Identification of a novel yeast caspase - Functional analysis of the raptor- protein regarding the regulation of autophagy and apoptosis. 5. Yeast as a model for neurodegeneration - Parkinson´s, Alzheimer`s, and Huntington`s disease. 6. Caspase independent regulation of cell death - implications of apoptosis-inducing factor AIF1 and its interactom in aging and apoptosis. Contact: Prof. Dr. Frank Madeo. ...

Author(s): Nisar R, Hanson PS, He L, Taylor RW, Blain PG, Morris CM. Publication type: Article. Publication status: Published. Journal: Archives of Toxicology. Year: 2015. Volume: 89. Issue: 10. Pages: 1811-1825. Print publication date: 01/10/2015. Online publication date: 19/02/2015. Acceptance date: 06/01/2015. Date deposited: 01/07/2015. ISSN (print): 0340-5761. ISSN (electronic): 1432-0738. Publisher: Springer. URL: http://dx.doi.org/10.1007/s00204-015-1453-5. DOI: 10.1007/s00204-015-1453-5. PubMed id: 25693864. ...

|strong|Rabbit anti Endonuclease G antibody|/strong| recognizes human endonuclease G, also known as endoG. Endo G is a 297 amino acid, including a 48 amino acid transit peptide ~36 kDa mitochond…

Proper activation of DRs is critical in regulation of T cell development and function. However, the nature of functional receptors, which are expressed on T cells at stages characterized by resistance to the impact of FasL, TNF, and TRAIL, remains to be established. In this study, we have shown that engagement of distinct epitopes on CD99 induced rapid death signaling in the majority of immature T cell lines examined, apparently by a caspase-independent pathway. In contrast, only a few T cell lines were distinctly responsive to apoptosis-inducing anti-Fas and TRAIL. Differences between the Ad20/CD99-mediated death pathway and other novel nonclassical apoptotic pathways were also observed. Thus, our data suggest that CD99 may be a major DR used by the immune system to control T cells at stages before they acquire susceptibility to the impact of death-mediating cytokines of the TNF superfamily.. Previous studies have shown that activation of the CD99 domain specified by mAbs O662, L129, and DN16 ...

LB-18 compound: synthetic drug closely related to lembehyne-A (derived from a marine sponge) induces caspase-independent cell death to human neuroblastoma cells; structure in first source

Parthanatos (a programmed necrosis from PARP1 hyperactivation) is an untapped death mechanism in oncology, and it offers some ideal features for cancer treatments including immunogenicity and its independence from the drug resistance-ridden p53-apoptosis ...

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Shop a large selection of products and learn more about Biomatik CorporationHuman Apoptosis Inducing Factor (AIF) ELISA Kit, 96 tests.

Meghna Nair is Human Resources Officer at AIFs India country office in Gurgaon.She holds a PGD in Human Resource & Administration from Tata Institute of Social Science -SVE .She has 5+ years of work experience in corporate as well as development sector. Prior to joining AIF in 2021, Meghna has worked with Rentickle.. ...

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Sequencing-based approaches have led to new insights about DNA methylation. While many different techniques for genome-scale mapping of DNA methylation have been employed, throughput has been a key limitation for most. To further facilitate the mapping of DNA methylation, we describe a protocol for gel-free multiplexed reduced representation bisulfite sequencing (mRRBS) that reduces the workload dramatically and enables processing of 96 or more samples per week. mRRBS achieves similar CpG coverage to the original RRBS protocol, while the higher throughput and lower cost make it better suited for large-scale DNA methylation mapping studies, including cohorts of cancer samples. ...

Among the candidate cancer-prognostic genes is the p53 tumor suppressor gene, which, when mutated, plays an important role in the development of many types of cancers. To facilitate robust large-scale DNA analysis of microdissected tumor biopsies, we describe a multiplex/nested PCR approach for a simultaneous outer amplification of exons 4-9 of the human p53 gene with parallel amplification of the HLA-DQB1 locus, involving a total of 14 primers. This approach reduces the required number of cells for analysis and avoids any variation in the amplifications of the individual p53 exons during the common outer amplification step. The HLA sequencing allows sample identification because the DQB1 locus is highly polymorphic and is thereby patient-specific. The p53 and HLA amplicons are analyzed by solid-phase sequencing in a semiautomated format. To improve the DNA sequence quality, we used 2-deoxyribonucleoside 5-O-1-thiotriphosphates in the sequencing reactions.. ...

The Genome Sciences Centre sequencing platform is a high-throughput large-scale DNA analysis facility that has been designed to maximize capacity while maintaining efficiency, scalability and flexibility. The platform is one of the largest platforms of its type in Canada and is well recognized internationally.. Current production scale capabilities of the capillary based platform include fosmid end sequencing, PCR amplicon sequencing, plasmid, and BAC end sequencing. We have two Applied Biosytems 3730xls yielding a capacity of 7680 lanes per week, or approx 6 million Q20 bases per week. The GSC sequencing platform prides itself on its flexibility and molecular biology enabling PCR, BAC, fosmid and plasmid DNA preps and several optimized reaction chemistries, allowing us to provide high-quality, high-yield, consistent data generation.. More information on the Illumina Platform.. Through our sequencing validation team we offer several high quality, high throughput targeted sequencing services. We ...

Expression of AIF1 (AIF-1, Em:AF129756.17, IBA1, IRT-1) in liver tissue. Antibody staining with HPA049234 in immunohistochemistry.

Looks like i did it guys! I finally made a build on my own that did a GR 70! (Note i have never done a GR 70 before but specifically wanted to design my own build to do it) I know it might share some similarities to other builds but ultimately the only thing other builds influenced is choosing Mirinae over Bane of the powerful. I needed the healing to keep me alive. I manged to do a GR70 with just over 5 minutes remaining and only 3 deaths. I dont really know how to do a build guide but i just focoused on CHC and CHD as well as max essence. Beyond that the skills and items speak for themselves.. ...

Ibutamoren MK-677 je silný tabletkový stimulant tvorby vlastného rastového hormónu (endogénneho HGH) a silne anabolického hormónu IGF-1 v tele. Športovci tento suplement využívajú predovšetkým pre jeho excelentnú schopnosť pomáhať budovať nové svalové prírastky (dosahovať pozitívnu dusíkovú bilanciu.

Sea icicles forming on undercut lip of the Drygalski Ice Tongue from wave action and freezing seawater, Ross Sea, Antarctica District, Antarctica Region, Antarctica stock photo. Quality New Zealand images by well known photographer Rob Suisted, Natures Pic Images.

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Pneumococcus is the most common and aggressive cause of bacterial meningitis and induces a novel apoptosis-inducing factor-dependent (AIF-dependent) form of brain cell apoptosis. Loss of production of two pneumococcal toxins, pneumolysin and H(2)O(2), eliminated mitochondrial damage and apoptosis. Purified pneumolysin or H(2)O(2) induced microglial and neuronal apoptosis in vitro. Both toxins induced increases of intracellular Ca(2+) and triggered the release of AIF from mitochondria. Chelating Ca(2+) effectively blocked AIF release and cell death. In experimental pneumococcal meningitis, pneumolysin colocalized with apoptotic neurons of the hippocampus, and infection with pneumococci unable to produce pneumolysin and H(2)O(2) significantly reduced damage. Two bacterial toxins, pneumolysin and, to a lesser extent, H(2)O(2), induce apoptosis by translocation of AIF, suggesting new neuroprotective strategies for pneumococcal meningitis ...

Its rare that we add a pony into our line-up, but the beautiful type that Harlequin gives his foals caught our attention. At just 12.2 hands, Woodburys Harlequin is an adorable option for sport pony breeders that are looking to emphasize jumping ability. His pedigree includes a high percentage of Welsh and Arabian blood, strongly consolidating his type and expression. Grandsire Neron stood in Holland and was approved with the NRPS. Neron was a very trainable and versatile talent, training both in dressage and jumping. Harlequins dam is herself a graded sports mare who has earned a Ster predicate. She was successful in both dressage, showjumping, and working hunter pony. Her sire, Moelview Mohawk, was highly successful showing in-hand and has produced both in-hand and undersaddle winners.. Harlequin was lightly shown in 2009 and 2010 with resounding success. He won 11 Champion or Reserve Champion placings in-hand and finished second at PoniesUK. Now being shown by a very young junior rider, ...

The SEA/GATOR complex is an essential regulator of the mTORC1 pathway. In mammals the GATOR1 complex is composed of the proteins DEPDC5, NPRL2 and NPRL3. GATOR1 serves as an mTORC1 inhibitor and activates the mTORC1-modulating RagA GTPase. However, several GATOR members have mTORC1 independent functions. Here we characterize mammalian cells overexpressing the GATOR1 component NPRL2. We demonstrate that, in the cells with active p53, ectopic expression of NPRL2 induces NOX2-dependent production of reactive oxygen species and DNA damage. Overexpressed NPRL2 accumulates in the nucleus, together with apoptosis-inducing factor (AIF). These events are accompanied by phosphorylation of p53, activation of a DNA-damage response and cell cycle arrest in G1 phase, followed by apoptosis. In the cells negative for active p53, NPRL2 ectopic expression leads to activation of CHK1 or CHK2 kinases and cell cycle arrest in S or G2/M phases. Combined, these results demonstrate a new role for the NPRL2, distinct from its

Interest in potential cancer chemopreventive and therapeutic properties of dietary ingredients, which are generally more tolerable in the human body ( 15), has increased in recent years, especially for the cancers that usually do not respond well to currently available therapies ( 17). Therefore, it is not surprising that chemoprevention of colon cancer, the leading cancer-related death in Western countries, is becoming more attractive. One of the dietary ingredients that possess anti-inflammatory and/or antioxidant properties is curcumin. Epidemiologic data suggest that curcumin may be responsible for the lower colon cancer rate in India and Southeast Asia ( 39). Based on that and its long history of consumption without any adverse health effects, curcumin is considered to be a safe chemopreventative agent ( 15).. Although it was suggested previously that curcumin-induced apoptosis is mediated through the impairment of the ubiquitin-proteasome pathway ( 40), the exact mechanism was not fully ...

Fingerprint Dive into the research topics of Molecular mechanisms of curcumin-induced cytotoxicity: Induction of apoptosis through generation of reactive oxygen species, down-regulation of Bcl-X,sub,L,/sub, and IAP, the release of cytochrome c and inhibition of Akt. Together they form a unique fingerprint. ...

Precisely blended botanicals to emulate Harlequins flavor & attributes found in nature!. Channel the all-day fun of the pantomiming street jester without losing too much edge on the sense of humor, with the sativa Harlequin. Super-high Myrcene counts allow for a focused and light-hearted experience without too much psychoactive, fast-thinking side effects, making it ideal for novice users or those looking for pain relief. ...

Expression of AIF1 (AIF-1, Em:AF129756.17, IBA1, IRT-1) in kidney tissue. Antibody staining with HPA049234 in immunohistochemistry.

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Mice and primary neuronal cultures. Hq, Apaf1-/-, and Bax-/- mice were maintained and genotyped as described previously (Fortin et al., 2001; Klein et al., 2002). Cortical neurons and cerebellar granule neurons (CGNs) were cultured from cortices of E15.5 mice and cerebellums of postnatal day 7 mice, respectively, as described previously (Fortin et al., 2001). Recombinant adenoviral vectors carrying human AIF or LacZ expression cassettes were prepared and used at 50 multiplicity of infection as described previously (Cregan et al., 2002).. Camptothecin treatment and cell viability assays. Neurons were treated with 10 μm camptothecin with or without 10 μm Boc-aspartyl (Ome)-fluoromethylketone (BAF) (Enzyme System Products, Livermore, CA) after 2 d in vitro (DIV). Cell survival was measured by the following: live/dead staining (Molecular Probes, Eugene, OR), Hoechst staining, MTT assay (Cell Titer kit; Promega, Madison, WI), terminal deoxynucleotidyl transferase-mediated biotinylated UTP nick end ...

These two grants laid the groundwork for investment from four additional awards. In October 2009, UW Medicine received a large-scale DNA sequencing project award from the National Heart, Lung, and Blood Institute (NHLBI), made under the auspices of the American Recovery and Reinvestment Act (ARRA) of 2009. The lions share, $25 million, has been used to launch the Northwest Genomics Center at UW Medicine. An additional $2 million grant from the states Life Sciences Discovery Fund is supporting the new centers infrastructure. Deborah A. Nickerson, professor of genome sciences and one of the centers principal investigators, says the grant recognizes UW Medicines expertise in genomics. Weve been working in the area of medical sequencing for quite some time, particularly in cardiovascular disease, says Nickerson.. The lions share, $25 million, has been used to launch the Northwest Genomics Center at UW Medicine. An additional $2 million grant from the states Life Sciences Discovery Fund is ...

Large-scale DNA molecular studies require reliable and efficient tools for DNA extractions. However, for some plant species and brown algae, isolation of high-quality DNA is difficult. We developed a novel method for isolating high-quality DNA from the polysaccharide-rich and polyphenol-rich brown algae based on a commercial kit and protocol (Qiagen) by optimizing the lysis step and including a chloroform/isoamyl alcohol supplementary purification step. DNAs from 24 brown algal species extracted using the original and the modified Qiagen protocol were compared for yield, quality, and effectiveness in PCR amplification. There was no significant difference in the yields between protocols. However, a statistically significant increase in DNA purity was obtained with the modified protocol, for which the A260/A280 and A260/A230 absorbance ratios averaged 1.66 ± 0.05 and 1.31 ± 0.01, respectively, compared to 1.37 ± 0.04 and 0.52 ± 0.04 with the original protocol. DNAs extracted by the modified ...

The objective of this study was to investigate altered expressions of nuclear matrix proteins (NMPs) of human osteosarcoma (OS) MG-63 cells during curcumin-induced apoptosis of human OS MG-63 cells. MG-63 cells were cultured with curcumin (7.5 mg/L) for 72 hr. Morphological alterations of cells were captured using light microscopy and transmission electron microscopy, and cell cycle distribution was estimated by flow cytometry. NMPs were selectively extracted and subjected to two-dimensional gel electrophoresis (2-DE) analysis. Western blots were performed to determine changes in the expression levels of specific NMPs. The results demonstrated that typical characteristics of apoptosis were observed. Cellular chromatin agglutinated, cell nuclei condensed, and apoptotic bodies were formed after treatment with curcumin. The 2-DE results displayed 27 NMPs, 21 of which were identified to have change in expression levels significantly during apoptosis. The altered expressions of three of these NMPs ...

Parkinsons disease is a common neurodegenerative disorder and the Dawson lab is studying the genetic basis of PD by investigating the mechanisms by which mutations in familial-linked genes cause PD, with hopes of identifying potential therapeutic targets for developing PD treatments. Current projects include the study of alpha-synuclein, LRRK2, parkin and PINK1.. Nitric oxide is a major player in neuronal cell death and the Dawson team has discovered parthanatos, a caspase-independent programmed cell death pathway involving apoptosis inducing factor (AIF) downstream of NO and its major target poly (ADP-ribose) polymerase (PARP). The team now is further characterizing that pathway to identify targets of AIF and the roles of other cell death effectors with the hope of identifying new signaling pathways that might be amenable to therapeutic intervention. In addition they are investigating the role of poly (ADP-ribose) as signaling molecule.. Representative Publications:. ...

Our Harlequin floor has been installed and we are still receiving the care that was promised. It is wonderful to see such great customer service even after the completion of the job! The team at Harlequin were an absolute pleasure to deal with. Extremely professional, caring and knowledgeable.. ...

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Pankaj Gupta, Zao zhong Su, Irina V. Lebedeva, Devanand Sarkar, Moira Sauane, Luni Emdad, Michael A. Bachelor, Steven Grant, David T. Curiel, Paul Dent, Paul B. Fisher ...

About two weeks ago, I came a across a Tweet that said Harlequin was having a pitch challenge for its Blaze line. If you dont know Harlequin, its a company...

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Lapin AIF1 Polyclonal anticorps AA 116-147 pour IF (p), IHC (fro), IHC (p), WB. Publier en 3 références Pubmed. Commandez anti-AIF1 anticorp. | Numéro de produit ABIN685477

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This summer a young friend attended summer camp at Pennsbury Manor, and during the course of the week she formed some opinions about my job as the Museum Educator. She told me that I have the coolest job in the world because I get to take care of the animals, give tours, and drive the golf cart. Well, maybe my job isnt quite as simple as that, but it is pretty cool! Without question, my favorite part of the job is talking with visitors. I get to learn where they are from and what brought them to Pennsbury Manor, hear their questions and discussing answers - because history is rarely made of pure facts. Most of all, I love that moment (especially transparent in children) when an idea catches hold and true learning takes place.. Every day, all sorts of people (including you!) visit our blog. I often feel like I am missing out because I cant have the same conversations with you as I do with the people who visit the physical site. But lately weve been having some great discussions with our ...