Site-directed mutagenesis and other molecular biology-based techniques are now available for probing the amphipathic alpha-helix structural motif in the exchangeable apolipoproteins. Here we survey the published literature on lipid-binding and functional domains in apolipoproteins A-I, A-II, A-IV, C-I, C-II, C-III, and E and compare these results with recently developed computer methods for analysis of the location and properties of amphipathic helixes. This comparison suggests that there are at least three distinct classes of amphipathic helixes (classes A, Y, and G*) in the exchangeable apolipoproteins whose distribution varies within and between the seven apolipoproteins. This comparison further suggests that lipid affinity resides largely in class A amphipathic helixes (Segrest, J. P., et al. 1990. Proteins. 8: 103) and that variations in structure and/or numbers of class A domains in individual apolipoproteins allow a range of lipid affinities from high to low. The positions of the four a ...
Gentaur molecular products has all kinds of products like :search , Alpha Dia \ Anti_Human Plasma Apolipoprotein A_I protein antiserum \ APOA11-S for more molecular products just contact us
Background: Insulin resistance is linked to dyslipidemia, characterized by a decrease in high density lipo-proteins and an increase in low density lipoproteins. Thiazolidinediones (TZDs) are insulin-sensitizing agents used to improve glycemic control in patients with type 2 diabetes. Recently, the safety of certain TZD regimens has been questioned because of associated adverse effects on the plasma lipid profile. We examined the effect of a TZD, Ciglitazone, on apolipoprotein synthesis and secretion in human liver HepG2 cells. Methods and Results: The effect of Ciglitazone treatment on apolipoprotein synthesis was addressed at the level of transcription, translation and secretion. RT-PCR showed that Ciglitazone increased the transcription of apoE and apoAI but reduced the levels of apoCI and apoB mRNA. Western blot analysis showed an increase in apoAI and apoE secreted in the cell culture media, whereas the amounts of apoB100 and apoCI were reduced. To confirm that Ciglitazone regulates apolipoprotein
Apolipoproteins have multiple roles. One role is to increase the overall solubility of the lipid particle, helping it to dissolve in the aqueous environment of the blood (apolipoproteins are amphipathic, or detergent-like proteins). Apolipoproteins can also function as enzyme co-factors (receptor ligands), facilitating the transfer of their lipid cargo to specific cells. Thus, the apoliproteins are the "smart" or working-end of the lipoprotein particle. The apolipoproteins dictate where the particles will dock and where they can bind, and in so doing the apolipoproteins regulate lipid metabolism in the body. So although the particles are composed of phospholipids and have lipid cargo, the few proteins on their surface are what give them their collective name of lipoproteins ...
Apolipoprotein L 5山羊多克隆抗体(ab79281)可与人样本反应并经WB, ELISA实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Peptide analogues of the class A amphipathic helixes from exchangeable apolipoproteins mimic apolipoprotein (apo) A-I in a number of ways, including the ability to activate the enzyme lecithin:cholesterol acyltransferase, to associate with high density lipoproteins (HDLs), and to form HDL-like particles in the presence of lipids. This study investigated the metabolic properties of several of these peptide analogues in the rat. Peptide analogues studied were 18A (referred to as L-18A to differentiate it from D-18A, and which mimics apolipoprotein amphipathic helical domains in its charge distribution), 37pA (a dimer of two 18A monomers separated by a proline), 18R (with reversed charge distribution compared with 18A), and D-18A (identical in amino acid sequence to 18A but synthesized from D-amino acids). Peptides were radiolabeled with 125I. In addition, metabolism of rat and human 125I-apo A-I and human 14C-apo A-I was studied; no significant differences in clearance of these preparations were ...
A novel apolipoprotein, designated ApoN, has been identified in bovine ovarian follicular fluid using chromatographic purification methods, amino acid sequence analysis, molecular biology, and bioinformatics. The apolipoprotein is a hydrophobic 12-kDa protein processed from the C terminus of a 29-kDa precursor expressed in a number of tissues, including the ovary, testis, the anterior chamber of the eye, skeletal muscle, uterus, and liver. Bovine, porcine, and murine ApoN display significant homology at the amino acid level across the entire precursor sequence. Surprisingly, there appears to be no orthologous protein in the human, although an APON-like pseudogene is found on chromosome 12. The N-terminal fragment of the ApoN precursor shows significant homology with the N-terminal sequence of the precursor of the cholesterol transport regulatory protein ApoF, but the corresponding C-terminal sequences of ApoN and ApoF possess no homology. ApoN is present in the high-density lipoprotein fraction ...
Apolipoprotein F山羊多克隆抗体(ab81908)可与人样本反应并经WB, ELISA, ICC实验严格验证。所有产品均提供质保服务,中国75%以上现货。
Apolipoproteins A: Structural proteins of the alpha-lipoproteins (HIGH DENSITY LIPOPROTEINS), including APOLIPOPROTEIN A-I and APOLIPOPROTEIN A-II. They can modulate the activity of LECITHIN CHOLESTEROL ACYLTRANSFERASE. These apolipoproteins are low in atherosclerotic patients. They are either absent or present in extremely low plasma concentration in TANGIER DISEASE.
Apolipoproteins have important structural and functional roles in several lipoprotein particles. Apolipoproteins regulate lipid metabolism, adipose tissue, and energy production and serve major...
TY - JOUR. T1 - Purification of biologically active apolipoproteins by chromatofocussing. AU - McLeod, Roger. AU - Lacko, Andras G.. AU - Pritchard, P. Haydn. AU - Frohlich, Jiri. PY - 1986/1/1. Y1 - 1986/1/1. N2 - Chromatofocussing has been used to isolate homogeneous apolipoproteins (apo) from human very-low-density lipoproteins and high-density lipoproteins with protein recovery of 70%. The inclusion of sulfhydryl-reducing agent (dithiothreitol) was required during solubilization of the lipoproteins (following delipidation) to achieve reproducible elution profiles. Removal of polyvalent buffers from apoproteins was rapidly accomplished on small columns of hydroxylapatite. The biological activity of purified apo AI and apo CII was confirmed by assessment of their ability to activate lecithin:cholesterol acyltransferase or lipoprotein lipase, respectively. Functional properties of isolated apo E were assessed by in vitro interaction with the low-density lipoprotein receptor expressed by ...
In this tissue microarray analysis of arterial apolipoprotein deposits, we found that vulnerable patients, that is, patients who experienced symptomatic atherosclerosis during their lifetime, have increased apoE and apoB deposits at the earliest lesional stages, that is, during intimal thickening or even in microscopically healthy arteries. The histomorphological pattern of apolipoprotein distribution in the arterial wall was nearly identical for apoE and apoB. As an unexpected finding, patients with diabetes mellitus had minimal intimal apolipoprotein deposits in early atherosclerosis as detected by immunohistochemistry.. The net amount of apolipoproteins in the arterial wall is the sum of delivery or influx, local synthesis or retention, and degradation or efflux. Our finding that apolipoproteins first accumulate in the arterial intima, that media deposits are found only when extensive intimal deposits are present, and that apolipoproteins are virtually absent from the adventitia supports the ...
High-density lipoprotein is predominantly composed of the apolipoproteins apoAI and apoAII. These apolipoproteins are responsible for collecting lipids from arteries and transporting them back to the liver for reutilization, which provides protection against cardiovascular diseases. While many studies examine the cardiovascular effects of HDL and its apolipoproteins, few have looked at whether these molecules maintain the health of other bodily systems and organs. In this study, the authors show that apoA1 maintains pulmonary function in mice. Along with inhibiting stressors such as proinflammatory HDL formation and the activity of paranoxonase 1 (PON1) and 3-nitrotyrosine (3NT) in the plasma, apoA1 was shown to limit pulmonary inflammation and oxidative stress markers, such as 3NT, 4-hydroxynonenal adducts (4-HNE), transforming growth factor-β (TGFβ), xanthineoxidase, myeloperoxidase and endothelial nitric oxide synthase in the lung milieu. Additionally, apoA1 was shown to enhance arterial ...
Complete information for APOL6 gene (Protein Coding), Apolipoprotein L6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Recombinant mammalian apolipoproteins have been expressed in a variety of cultured mammalian cell lines (Reardon et al. 1986; Mallory et al. 1987; Blackhart et al. 1990; Yao et al. 1991), transgenic...
Principal Investigator:Saito Hiroyuki, Project Period (FY):2013-04-01 - 2017-03-31, Research Category:Grant-in-Aid for Scientific Research (B), Section:一般, Research Field:Physical pharmacy
Abstract. Apolipoprotein M (APOM) has been suggested as a vasculoprotective constituent of high density lipoprotein (HDL), which plays a crucial role behind the mechanism of HDL-mediated anti-atherosclerosis. Previous studies demonstrated that insulin resistance could associate with decreased APOM expressions. In agreement with our previous reports, here, we further confirmed that the insulin sensitivity was also reduced in rats treated with high concentrations of glucose; such effect could be reversed by administration of rosiglitazone, a peroxisome proliferator-activated receptor-γ (PPARγ). The present study shows that Apom expression is significantly affected by either rosiglitazone or hyperglycemia alone without cross interaction with each other, which indicates that the pathway of Apom expression regulating by hyperglycemia might be differed from that by rosiglitazone. Further study indicated that hyperglycemia could significantly inhibit mRNA levels of Lxrb (P=0.0002), small heterodimer ...
Article: We report studies of the interaction of Alzheimers amyloid beta protein (Aβ) with normal human plasma high density lipoprotein (HDL), aiming to clarify to which lipoprotein (LP) structural constituent (apolipoprotein or lipid) soluble Aβ is primarily bound. Purified HDLs were incubated wit...
The HDL associated apolipoprotein M (apoM) protects against experimental atherosclerosis but the mechanism is unknown. ApoM increases prebeta-HDL formation. We explored whether plasma apoM affects mobilization of cholesterol from peripheral cells in mice ...
Our results are the first demonstration of a defined signal sequence as a distinct, separate entity within the circulation in humans. Whereas Christofferson et al18 reported that the SP of apolipoprotein M is retained along with the entire apolipoprotein M protein in the circulation of humans and that this uncleaved SP helps prevent renal filtration of apolipoprotein M through hydrophobic association with circulating lipoproteins, they did not detect the apolipoprotein M SP as a distinct, separate entity. We found no evidence on immunoassay or HPLC for the SP fragment BNPsp (17-26) in human plasma to be associated with proBNP (1-108), ie, as a component of preproBNP (1-134). Nevertheless, we cannot exclude the existence of such an association because our BNPsp (17-26) assay is designed to detect free carboxyl terminal residues in position 26 of the SP, which requires cleavage of proBNP (1-108). Future work with assays directed toward the amino terminal region of preproBNP (1-134) is needed to ...
Apolipoprotein F (apoF) is a 35 kDa protein encoded by a cDNA cloned from the murine lacrimal gland. In the present paper, the murine apoF has been expressed as a recombinant histidine-tagged protein in Escherichia coli and purified from expressing cultures. We report here the unique distinctions of apoF including comparisons of apoF with other apolipoproteins (apoD, apoE, and apoN), as well as why this protein was produced. The data presented here provide evidence that isolated recombinant apoF purified in the experimental conditions described here can be used for functional studies ...
Polyclonal antibodies raised against a range of seed apolipoproteins from the family Cruciferae have been used for the first time for low resolution epitope characterisation. Antibodies were raised against the major seed apolipoproteins of Brassica napus, Sinapis alba and Raphanus sativum. In each case, the antibodies recognized, in addition to the 19-20 kDa apolipoprotein to which they were raised, similar 19-20 kDa apolipoproteins from a wide range of species in the family Cruciferae, but not in other plant families. Homologous or heterologous two-sites (sandwich) assays were performed with the format [antibody A - test apolipoprotein - antibody B - 2° antibody]. The results showed a drastically reduced antibody B binding by apolipoproteins preincubated with an antibody A. This indicated the presence of a single major epitope on many of the apolipoproteins. The antigenicity of native and denatured apolipoproteins was similar, although the antigenicity of the former was much more readily ...
TY - JOUR. T1 - Gender and ethnic differences in a case-control study of dyslipidemia. T2 - using the apolipoprotein A-V gene as an exemplar in cardiovascular genetics.. AU - Wung, Shu Fen. AU - Aouizerat, Bradley. PY - 2003. Y1 - 2003. N2 - Common, complex genetic disorders such as coronary heart disease (CHD) frequently show large population differences, contributing to health disparities. It is also well known that CHD risk factor profiles and the frequency of coronary events differ by gender. Study of premature CHD has revealed that apolipoproteins are important discriminating factors for distinguishing individuals with CHD. Recent findings indicated that apolipoprotein A-V (APOA-V) gene promoter polymorphisms are an important determinant of plasma triglycerides (TG) and lipoprotein cholesterol, and a risk factor for CHD. Variations in APOA-V may have varying impacts in different ethnic groups. The purpose of this interdisciplinary genetic research project was to determine (1) the ...
Mouse Monoclonal Anti-Apolipoprotein M/ApoM Antibody (8F12C6B8) [Alexa Fluor® 488]. Validated: WB, ELISA, ICC/IF. Tested Reactivity: Human. 100% Guaranteed.
Monoklonale und polyklonale Apolipoprotein M Antikörper für viele Methoden. Ausgesuchte Qualitäts-Hersteller für Apolipoprotein M Antikörper. Hier bestellen.
Apolipoproteins are proteins that bind lipids (oil-soluble substances such as fat and cholesterol) to form lipoproteins. They transport the lipids through the lymphatic and circulatory systems. The lipid components of lipoproteins are insoluble in water. However, because of their detergent-like (amphipathic) properties, apolipoproteins and other amphipathic molecules (such as phospholipids) can surround the lipids, creating the lipoprotein particle that is itself water-soluble, and can thus be carried through water-based circulation (i.e., blood, lymph). Apolipoproteins also serve as enzyme cofactors, receptor ligands, and lipid transfer carriers that regulate the metabolism of lipoproteins and their uptake in tissues. In lipid transport, apolipoproteins function as structural components of lipoprotein particles, cofactors for enzymes and ligands for cell-surface receptors. In particular, apoA1 is the major protein component of high-density lipoproteins; apoA4 is thought to act primarily in ...
DES regulates expression of avian apolipoprotein D during regression and recrudescence of the oviduct and epithelial-derived ovarian carcinogenesis.
Complete information for APOM gene (Protein Coding), Apolipoprotein M, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Apolipoproteins from human plasma high density (HDL), low density (LDL) and very low density lipoproteins (VLDL) were visualized in human arteries employing immunofluorescence techniques. Comparison between the localization patterns in extracranial and intracranial arteries and those in coronary arteries and the aorta was made. ApoA-I from HDL, apoB from LDL, and apoC-III from VLDL, as well as neutral lipid, were all localized to connective tissue and extracellular lipid pools in atherosclerotic lesions, and only to areas of intimal thickening in grossly "uninvolved" arteries. The degree of superposition of localizations was similiar in each vascular bed, and within the error resulting from the structural changes due to the focal nature of the atherosclerotic process. These results suggest a broad specificity in localization of apolipoproteins in most arterial lesions, and suggest that no differences in apolipoprotein accumulation exist between extracranial and intracranial arteries, coronary ...
Humans resist infection by the African parasite Trypanosoma brucei owing to the trypanolytic activity of the serum apolipoprotein L1 (APOL1). Following uptake by endocytosis in the parasite, APOL1 forms pores in endolysosomal membranes and triggers lysosome swelling. Here we show that APOL1 induces both lysosomal and mitochondrial membrane permeabilization (LMP and MMP). Trypanolysis coincides with MMP and consecutive release of the mitochondrial TbEndoG endonuclease to the nucleus. APOL1 is associated with the kinesin TbKIFC1, of which both the motor and vesicular trafficking VHS domains are required for MMP, but not for LMP. The presence of APOL1 in the mitochondrion is accompanied by mitochondrial membrane fenestration, which can be mimicked by knockdown of a mitochondrial mitofusin-like protein (TbMFNL). The BH3-like peptide of APOL1 is required for LMP, MMP and trypanolysis. Thus, trypanolysis by APOL1 is linked to apoptosis-like MMP occurring together with TbKIFC1-mediated transport of ...
Chemicals / CAS: lipoprotein lipase, 83137-80-8, 9004-02-8; APOA5 protein, human; Apolipoproteins A; Apolipoproteins; Triglycerides ...
Common DNA sequence variants rarely have a high-risk association with a common disease. When such associations do occur, evolutionary forces must be sought, such as in the association of apolipoprotein L1 (APOL1) gene risk variants with nondiabetic kidney diseases in populations of African ancestry. The variants originated in West Africa and provided pathogenic resistance in the heterozygous state that led to high allele frequencies owing to an adaptive evolutionary selective sweep. However, the homozygous state is disadvantageous and is associated with a markedly increased risk of a spectrum of kidney diseases encompassing hypertension-attributed kidney disease, focal segmental glomerulosclerosis, human immunodeficiency virus nephropathy, sickle cell nephropathy, and progressive lupus nephritis ...
ProSpecs Apolipoproteins include: Clusterin Human Recombinant, Clusterin Rat Recombinant, Apolipoprotein-D Human Recombinant, Human Apolipoprotein-J, Apolipoprotein-J Canine Recombinant
Apolipoproteins are proteins which bind to lipids, forming lipoprotein complexes to encapsulate cholesterol and triglycerides. Structure and transport of lipoproteins are regulated by the binding of specific apolipoproteins where they serve as receptors and ligands. Apolipoprotein C-3 (ApoC3) inhibits hydrolysis of lipids by lipase. Elevated levels of ApoC3 are associated with hypertriglyceridemia and an increased risk of coronary heart disease. Despite its importance in lipid metabolism, fundamental lipid binding properties of ApoC3 remain to be characterized. Using unilamellar vesicles with varied lipid composition and curvature, we apply single-liposome microscopy, circular dichroism spectroscopy, and transmission electron microscopy to determine membrane binding affinity, secondary structure formation, and membrane integrity, respectively. back to top. ...
Explore the science and understand more clearly the Apolipoprotein H topic by reading the best articles made from Experts, Scientific and Academics!
Trying to learn ? We can help! Memorize these flashcards or create your own flashcards with Cram.com. Learn a new language today.
UCL Discovery is UCLs open access repository, showcasing and providing access to UCL research outputs from all UCL disciplines.
Expression of APOO (FAM121B, MGC4825, Mic23, MIC26, My025) in cervix, uterine tissue. Antibody staining with HPA003187 in immunohistochemistry.
OBJECTIVE To examine whether the apolipoprotein M (APOM) rs805297 G/T polymorphism is associated with risk of rheumatoid arthritis (RA) in a Chinese population. METHODS We studied APOM rs805297 G/T gene polymorphism in 520 RA patients, and 520 controls in a Chinese population. Genotyping was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The blood plasma concentration of APOM was measured by an enzyme-linked immunosorbent assay in 84 RA patients and 84 controls. RESULTS When the APOM rs805297 G/T GG homozygote genotype was used as the reference group, the TT or GT/TT genotype was associated with an increased risk for RA (TT vs. GG, adjusted odds ratio 1.76, 95% CI 1.11-2.77, P=0.016; GT + TT vs. GG, adjusted odds ratio 1.30, 95% CI 1.02-1.67, P=0.037). The average concentration of APOM in plasma was significantly higher in RA patients compared to controls. Stratification analysis found a significantly increased risk for RA associated with the APOM rs805297 TT
Apolipoprotein M antibody [3H3] (apolipoprotein M) for IHC-P, WB. Anti-Apolipoprotein M mAb (GTX84882) is tested in Human samples. 100% Ab-Assurance.
INVOLVED IN lipid transport (inferred); lipoprotein metabolic process (inferred); FOUND IN extracellular region (inferred); INTERACTS WITH 2,3,7,8-tetrachlorodibenzodioxine; 6-propyl-2-thiouracil; buspirone
Abstract: Enzyme-linked immunosorbent assay (ELISA) has been used formeasuring apolipoproteins A-I and B in the urine. ApoB is absentin urine of healthy subjects, and apoA-I is determined in tracequantity. In patients with chronic glomerulonephritis quantity ofapoA-I in urine was 117 times as much as in control group. ApoBis present in urine of patients in considerable quantity(1528*315 *g/l).) The ELISA method for determining apoA-I andapoB in urine makes it possible to evaluate the gravity ofpathological process in kidney ...
Multiple variants of the AMBER all-atom force field were quantitatively evaluated with respect to their ability to accurately characterize helix-coil equilibria in explicit solvent simulations. Using a global distributed computing network, absolute conformational convergence was achieved for large ensembles of the capped A21 and Fs helical peptides. Further assessment of these AMBER variants was conducted via simulations of a flexible 164-residue five-helix-bundle protein, apolipophorin-III, on the 100 ns timescale. Of the contemporary potentials that had not been assessed previously, the AMBER-99SB force field showed significant helix-destabilizing tendencies, with beta bridge formation occurring in helical peptides, and unfolding of apolipophorin-III occurring on the tens of nanoseconds timescale. The AMBER-03 force field, while showing adequate helical propensities for both peptides and stabilizing apolipophorin-III, (i) predicts an unexpected decrease in helicity with ALA→ARG+ substitution, (ii)
Apolipoproteins are carrier proteins that bind lipids to form water-soluble lipoprotein particles that can be carried through blood and lymph. Several different classes and subclasses of apolipoproteins are known. Apolipoprotein B (ApoB) is the primary apolipoprotein in chylomicrons (lipoprotein particles that contain triglycerides, phospholipids, cholesterol, and proteins) and low-density lipoprotein (LDL). It is also known as FLDB and LDLCQ4. High levels of ApoB can lead to plaques that cause atherosclerosis, and ApoB levels can be a better indicator of heart disease risk than total cholesterol or LDL. The APOB transcript is subject to tissue-specific RNA editing, resulting in two major isoforms, ApoB-100 and ApoB-48.. ...
Apolipoproteins are carrier proteins that bind lipids to form water-soluble lipoprotein particles that can be carried through blood and lymph. Several different classes and subclasses of apolipoproteins are known. Apolipoprotein B (ApoB) is the primary apolipoprotein in chylomicrons (lipoprotein particles that contain triglycerides, phospholipids, cholesterol, and proteins) and low-density lipoprotein (LDL). It is also known as FLDB and LDLCQ4. High levels of ApoB can lead to plaques that cause atherosclerosis, and ApoB levels can be a better indicator of heart disease risk than total cholesterol or LDL. The APOB transcript is subject to tissue-specific RNA editing, resulting in two major isoforms, ApoB-100 and ApoB-48.. ...
Introduction: Almonds have been studied in the context of blood cholesterol-lowering diets, which consistently show beneficial effects on CVD risk factors. The independent effects of almonds beyond the contributions of a heart healthy diet have not been evaluated.. Hypothesis: A cholesterol-lowering diet with 1.5 oz. /d almonds will elicit greater cardioprotective effects on lipids, lipoproteins, and apolipoproteins than the same cholesterol-lowering diet with an isocaloric muffin substitution.. Methods: A randomized, 2-period, crossover, controlled feeding study was designed to test the effects of 1.5 oz. /d almonds compared to a calorie-matched, high carbohydrate snack. All foods provided were exactly the same in both diets except for the snack (almonds or muffin; 245 kcal/d). Thus, differences in the nutrient profiles of the control diet (59.3% CHO, 15.4% PRO, 25.2% FAT, no almonds/d) and almond diet (51.8% CHO, 16.7% PRO, 31.5% FAT, 1.5 oz. of almonds/d) were due to the nutrient profile ...
APOL4 - APOL4 (GFP-tagged) - Human apolipoprotein L, 4 (APOL4), transcript variant a available for purchase from OriGene - Your Gene Company.
BACKGROUND: Approximately 13% of black individuals carry 2 copies of the apolipoprotein L1 (APOL1) risk alleles G1 or G2, which are associated with 1.5- to 2.5-fold increased risk of chronic kidney disease. There have been conflicting reports as to whether an association exists between APOL1 risk alleles and cardiovascular disease (CVD) that is independent of the effects of APOL1 on kidney disease. We sought to test the association of APOL1 G1/G2 alleles with coronary artery disease, peripheral artery disease, and stroke among black individuals in the Million Veteran Program. METHODS: We performed a time-to-event analysis of retrospective electronic health record data using Cox proportional hazard and competing-risks Fine and Gray subdistribution hazard models. The primary exposure was APOL1 risk allele status. The primary outcome was incident coronary artery disease among individuals without chronic kidney disease during the 12.5-year follow-up period. We separately analyzed the cross-sectional ...
Apo-A1 is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. Apo-A1, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This Apo- 1 ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of Apo-A1 has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of Apo-A1 (called Apo-A1 Milano). The availability of recombinant normal Apo-A1 should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. Recombinant human Apo-A1 is a 28.2 kDa protein of 244 amino acid residues ...
Saha, N.,Kamboh, M.I.,Kelly, L.J.,Ferrell, R.E.,Tay, J.S. (1992). Apolipoprotein H (beta-2-glycoprotein I) polymorphism in Asians.. Human Biology 64 (4) : 617-621. [email protected] Repository ...