Expression of viral antigen in target tissues of mice 3-7 days after in vivo infection.Spleen and bone marrow cells were isolated from mice infected by the s.
The E.Coli derived recombinant artificial mosaic protein contains two epitopes from C-end associated in one molecule from the HHV-4 p18 regions.
BioAssay record AID 288762 submitted by ChEMBL: Inhibition of TPA-induced Epstein-Barr virus early antigen activation assessed as EBV-EA induction in Raji cells at 3.2 nM after 48 hrs relative to TPA.
Epstein-Barr virus early antigen: synthesized before or in the absence of viral-progeny DNA replication & present only in infected cells
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Browse our Epstein-Barr Virus product listings from our online selection of Antigens for research and diagnostics. ViroGen Corporation is a leading provider of antigens and antibodies to the research and diagnostics markets. Learn more about our service offerings and browse our products.
Immunotherapeutic treatments in head and neck cancer clinical trials include cancer vaccines targeting foreign viral antigens or mutational neoantigens derived from cancer-expressed proteins. Anti-tumor immune responses place cancer cells under selective pressure to lose or downregulate target antig …
Over the past 15 years, a number of observations have culminated in the localization of viral antigens in liver tissue by the use of routine histology, immunohistochemistry, electron microscopy (EM),...
The role of Epstein-Barr virus (EBV) early antigen diffuse component (EA-D) and its relationship with EBV DNA polymerase in EBV genome-carrying cells are unclear, EBV-specified DNA polymerase was purified in a sequential manner from Raji cells treated with phorbol-12,13-dibutyrate and n-butyrate by phosphocellulose, DEAE-cellulose, double-stranded DNA-cellulose, and blue Sepharose column chromatography. Four polypeptides with molecular masses of 110,000, 100,000, 55,000, and 49,000 daltons were found to be associated with EBV-specified DNA polymerase activity. A monoclonal antibody which could neutralize the EBV DNA polymerase activity was prepared and found to recognize 55,000- and 49,000-dalton polypeptides. An EA-D monoclonal antibody, R3 (G. R. Pearson, V. Vorman, B. Chase, T. Sculley, M. Hummel, and E. Kieff, J. Virol. 47:183-201, 1983), was also able to recognize these same two polypeptides associated with EBV DNA polymerase activity. It was concluded that EBV EA-D polypeptides, as ...
Summary The mol. wt. of the polymorphic Epstein-Barr virus (EBV) nuclear antigen (EBNA) molecule (EBNA 1) encoded by the BamHI K fragment of the EBV DNA has been determined in 14 EBV-carrying lymphoblastoid and Burkitt's lymphoma cell lines. There is no obvious correlation between the size of this polypeptide and any properties of the cells from which it is derived, other than those related to the strain of transforming virus. We confirm that the polymorphic region of this molecule is the glycine-alanine copolymer encoded by the third internal repeat of the EBV genome (IR3) and we consider the significance of this domain.
Immobilization of Raji cells on surface coated with anti-lymphocyte globulin (ALG) at low cell densities lead to the synthesis of Epstein-Barr virus (EBV) early antigen (EA) in up to 5% of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. Addition of iododeoxyuridine (IdUrd) to low density cultures increased the expression of EA to 20%, whereas in confluent cultures the cells could not be induced to synthesize EA. Treatment of cells in suspension with ALG failed to induced EA synthesis and did not potentiate the effect of IdUrd. Immobilized Raji cells proved to be suitable targets for superinfection with EBV derived from P3HR1 cultures. ...
Intended Use The ZEUS IFA Epstein-Barr Virus Early Antigen (EBV-EA) IgG Test System is an indirect fluorescent antibody assay intended for the qualitative and quantitative detection of antibodies to the early antigen (EA) of Epstein-Barr virus (EBV) in human sera. This test system detects IgG antibodies to diffuse (D) and restricted (R) components of the EBV-EA complex. This test system is for research use only. Product Description This test system uses methanol-fixed slides. This test system uses ZORBA NS Sample Diluent. Kit Size 25 x 10 wells
This study will examine the effects of long-term antiviral therapy with valaciclovir (Valtrex) on Epstein-Barr virus infection. This virus infects more
PubMed journal article: High prevalence of immunoglobulin A antibody against Epstein-Barr virus capsid antigen in adult patients with lupus with disease flare: case control studies. Download Prime PubMed App to iPhone, iPad, or Android
An effective method for the synthesis of 7,8-epoxy-1,3,11-cembratriene-15R(α), 16-diol and its in vitro Epstein-Barr Virus Early Antigen (EBV-EA) Activation Chemopreventive Assay are reported. This semisynthetic product is a new cembranoid with a potent tumor inhibitory activity that is expected to be a lead compound for a new class of chemopreventive agents of marine origin.
The government never tested the quality or effectiveness of the rapid antigen tests for Covid-19 recently introduced into the countrys testing policy, Le Soir
ID:1,Note:Change of Day(s) Test Setup to every Monday, Wednesday and Friday w.e.f 1 August 2014. ,Date:2014-07-29T08:45:00.000Z,Deleted:false,IsNew:true ...
The overriding performance driver for antigen capture microarrays is the functional integrity of the capture ligand on the surface of the assay device.
The numerous cases which were treated showed a positive clinical response in greater or lesser degree. The earliest cases were studied intensively and were readied for publication.. The tumors that responded to a greater degree to our treatment, as was expected, are the ones with the greatest degree of undifferentiation; because of their antigenic characteristics and because of the greater degree of dissimilarity between them and the genotypes and antigenic characteristics of normal cells, they have more probability of eliciting an efficient immunologic response.. Histological studies which were made give us certain possible mechanisms of action which correlate with the clinical response obtained.. Microscopic studies showed the almost complete delimitation of the tumoral areas by connective-vascular structures which seem to have diverse morphologies according to their proximity to the tumor and the level of their chronological development, having at first a thick endothelial wall with ...
Nagpahayag ng pagkabahala si Senador Nancy Binay sa plano ng gobyerno na gamitin ang rapid antigen test sa layuning mapalakas ang pagtukoy sa mga taong may COVID-19 sa bansa.
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We assessed the value of the cytomegalovirus (CMV) antigenemia assay for diagnosing primary CMV infection in adults. The CMV antigenemia assay was performed for 40 patients admitted to our unit over a 2-year period with unexplained fever and suspected primary CMV infection. Nine of the 10 patients with primary CMV infection had positive CMV antigenemia assays, and the results were available within 5 hours. All 10 patients had a mononucleosis-like syndrome. All but one of the 30 other patients had negative CMV antigenemia assays. A false-positive result was obtained for a patient with systemic lupus erythematosus. Overall, the CMV antigenemia assay was 90% sensitive and 96% specific for the diagnosis of primary CMV infection. Therefore, the CMV antigenemia assay appears to be a simple, rapid, inexpensive test for the diagnosis of primary CMV infection in hospitalized adults.. ...
The Epstein-Barr Virus Capsid Antigen (VCA) IgM ELISA Kit is intended for the measurement of IgM antibodies to Epstein-Barr Virus Capsid Antigen (VCA) in a sample. This kit utilizes Epstein-Barr VCA antigen (P3H3 cell extract, cultured in human Burkitt lymphoma cells).
The latency-associated nuclear antigen (LANA) of Kaposis sarcoma-associated herpesvirus functions as an origin-binding protein (OBP) and transcriptional regulator. LANA binds the terminal repeats via the C-terminal DNA-binding domain (DBD) to support latent DNA replication. To date, the structure of LANA has not been solved. Sequence alignments among OBPs of gammaherpesviruses have revealed that the C terminus of LANA is structurally related to EBNA1, the OBP of Epstein-Barr virus. Based on secondary structure predictions for LANA(DBD) and published structures of EBNA1(DBD), this study used bioinformatics tools to model a putative structure for LANA(DBD) bound to DNA. To validate the predicted model, 38 mutants targeting the most conserved motifs, namely three alpha-helices and a conserved proline loop, were constructed and functionally tested. In agreement with data for EBNA1, residues in helices 1 and 2 mainly contributed to sequence-specific DNA binding and replication activity, whilst ...
TY - JOUR. T1 - Synthetic peptides deduced from the amino acid sequence of Epstein‐Barr virus nuclear antigen 6 (EBNA 6). T2 - Antigenic properties, production of monoreactive reagents, and analysis of antibody responses in man. AU - Falk, K.. AU - Linde, A.. AU - Johnson, D.. AU - Lennette, E.. AU - Ernberg, I.. AU - Lundkvist, A.. PY - 1995/8. Y1 - 1995/8. N2 - Studies on the antibody responses to various Epstein‐Barr virus (EBV) antigens have been instrumental in the understanding of the seroepidemiology and diagnosis of this viral infection and the subsequent carrier state. While antibodies to the viral capsid antigen (VCA), early antigen (EA), and nuclear antigens 1 and 2 (EBNA 1 and 2) have been well characterized, the antibody response to the other nuclear antigens is not well understood. EBNA 6 is expressed by lymphoblasts during acute EBV infection and may be an important antigen for diagnosis and evaluation of the immune response. In order to analyze the antibody response to EBNA ...
Epithelial-mesenchymal transition is an important mechanism in cancer invasiveness and metastasis. We had previously reported that cancer cells expressing Epstein-Barr virus (EBV) latent viral antigens EBV nuclear antigen EBNA3C and/ or EBNA1 showed higher motility and migration potential and had a propensity for increased metastases when tested in nude mice model. We now show that both EBNA3C and EBNA1 can modulate cellular pathways critical for epithelial to mesenchymal transition of cancer cells. Our data confirms that presence of EBNA3C or EBNA1 result in upregulation of transcriptional repressor Slug and Snail, upregulation of intermediate filament of mesenchymal origin vimentin, upregulation of transcription factor TCF8/ZEB1, downregulation as well as disruption of tight junction zona occludens protein ZO-1, downregulation of cell adhesion molecule E-cadherin, and nuclear translocation of β-catenin. We further show that the primary tumors as well as metastasized lesions derived from EBV ...
There is increasing interest in formulating an effective vaccine against EBV, designed to not only limit the outgrowth of latently infected B cells in healthy individuals but to also block the development of many EBV-associated malignancies such as Burkitts lymphoma, nasopharyngeal carcinoma, and Hodgkins disease. In western societies, the principle aim of such a vaccine would be to protect from IM. In this context, virus load (a large dose of orally transmitted virus and/or overexpansion of the virus-transformed B cell pool beyond a critical threshold) may be a critical determinant of disease risk 1 . Therefore, a vaccine capable of either blocking primary EBV infection or significantly reducing the EBV load during primary infection may be adequate to avert clinical symptoms. A similar vaccine will also be able to reduce the immediate risk of lymphoproliferative disease in transplant patients receiving immunosuppressive therapy. On the other hand, EBV-associated malignancies such as Burkitts ...
The various antigen complexes of the Epstein-Barr virus (EBV) are broadly classified as the viral capsid antigen (VCA), diffuse early antigen (EA-D), restricted early antigen (EA-R), membrane antigen (MA) and the Epstein-Barr nuclear antigen (EBNA). The different EBV-related diseases may be differentiated according to the reactivity of these different classes of antibodies towards the various classes of antigen complexes. However, with the recent development of molecular biology, it is now known that the individual polypeptides of the different EBV antigen complexes can be used as serological markers for the detection of nasopharyngeal carcinoma (NPC). Among the useful serological markers which have been used in enzyme-linked immunosorbent assay (ELISA) for the detection of NPC are the gp125 from the VCA complex (IgA), pp58 from the EA-D complex (IgG), ribonucleotide reductase (IgG and IgA), DNase (IgA) and thymidine kinase (IgA) from the EA-R complex, gp 250/200 from the MA complex (IgA) and ...
Thane, Jul 13(PTI) Maharashtra Minister Eknath Shinde on Monday commissioned a programme in Thane city to administer rapid antigen tests to detect COVID-19 quickly. The Thane Municipal Corporation has placed an order for one lakh rapid antigen test kits, which can give results in 30 minutes, an official said, adding that tests will be conducted on a large-scale in hotspots, slum clusters etc using these kits. Municipal Commissioner Vipin Sharma said the increase in cases was because of the rise in number of tests, adding that 80 per cent of patients in the city were asymptomatic. During the function held in the TMC headquarters, Shinde also launched Mission Zero campaign to contain the outbreak. The campaign will be jointly carried out by TMC, MCHI CREDAI, Indian Jain Association and NGO Desh Apnaye. As part of the campaign, nine mobile dispensaries will make the rounds of nine wards. PTI COR BNM BNM ...
Blog on CMV antigen product: The CMV n/a (Catalog #MBS318657) is an Antigen produced from HF Cells and is intended for research purpos...
About Rapid Antigen Test Safe and private 100% service Best price for a home service Results available in Spanish and English Qualified personnel and biosafety protocols COVID-19 tests valid to enter any country and endorsed by INDRE and COFEPRIS. This rapid COVID-19 antigen test is performed using a nasapharyngeal swab and produces results in 15 minutes. Once the result has been observed, it will be broadcast in an electronic version within 3 successive hours.
Lee et al (87), used a high-throughput genotyping platform to determine the mutation status of 474 hotspots in 41 genes using 237 gastric adenocarcinomas, which included 58 EBVaGCs. Among these, 34 cases (14.3%) harbored somatic mutations, 6 of which concomitantly had two different mutations. Fourteen EBVaGC cases had mutations; 6 in PIK3CA (10.3%), 1 in p53 (1.7%), 2 in APC (3.4%), 1 in STK11 (1.7%), 3 in CTNNB1 (5.2%) and 1 in CDKN2A (1.7%). CTNNB1 mutations were significantly more frequent in EBVaGC than in EBV-negative gastric carcinomas (one of 179 cases, 0.6%). Frequent PIK3CA mutations were also reported in two subsequent studies; 16.7% (of 18 EBVaGCs) in a report by Sukawa et al (88), and 80% (of 28 EBVaGCs) in a report by The Cancer Genome Atlas (TCGA) Research Network (89). A recent report by Liang et al (90), showed several newly identified mutations in EBVaGC, including mutations in MAP3K4 (20.8%), TGFBR1 (25.0%), CCNA1 (25.0%) and AKT2 (38.2%). Among these, an AKT2 mutation was ...
The envelope glycoproteins of HIV, gp120 and gp41, contain epitopes recognized by neutralizing antibodies. Studies of human sera from infected individuals indicate that group-specific neutralization antigens common to most isolates of HIV-1 exist, and that some HIV-2 antisera cross-neutralize HIV-1. Neutralization epitopes for HIV-1 have been identified and mapped, including a group-specific antigen on gp41, and a type-specific antigen on gp120. Neutralization escape mutants have been selected in vitro with a neutralizing mab to the type-specific antigenic loop. The CD4 antigen binds HIV-1 gp120 with high affinity and acts as the receptor on human and simian T-lymphocytes and monocytes for all strains of HIV-1, HIV-2, and SIV tested. Following binding to the CD4 receptor, HIV becomes internalized by a pH-independent process. The principle binding domain for gp120 is located in the N-terminal V domain of CD4. Anti-idiotypic sera to CD4 mabs recognizing the same site weakly neutralize HIVs of many
5GTC: Crystal structure of complex between DMAP-SH conjugated with a Kaposis sarcoma herpesvirus LANA peptide (5-15) and nucleosome core particle
Ukrainian Deputy Health Minister and Chief Medical Officer Viktor Liashko says the ministry plans to boost COVID-19 testing by applying rapid antigen tests. According to Liashko, four manufacturers have already been incorporated in the United States.
HCAOA Associate Member Community Attire has COVID-19 Rapid Antigen Tests in in stock. These tests are intended for use in patient care settings by medical professionals with a CLIA waiver, Certificate of Compliance, or Certificate of Accreditation. Contact Community Attire at 213-761-4229 or [email protected] for details and to order ...
After washing to remove non-specifically bound antibodies, the substrate is incubated with an anti-human antibody conjugated to fluorescein. When results are positive, a stable three-part complex forms, consisting of fluorescent antibody bound to human antinuclear antibody that is bound to nuclear antigen. This complex can be visualized with the aid of a fluorescent microscope. In positive samples, the cell nuclei will show a bright apple-green fluorescence with a staining pattern characteristic of the particular nuclear antigen distribution within the cells. If the sample is negative for ANA, the nucleus will show no clearly discernible pattern of nuclear fluorescence. The cytoplasm may demonstrate weak staining while the non-chromosome region of mitotic cells demonstrates brighter staining ...
Proceeding/Conference:Biennial Conference of the International Association for Research on Epstein-Barr Virus and Associated Diseases ...
Considering the need for COVID-19 PCR TESTING ON PERSONAL REQUEST AT THE MICROBIOLOGICAL DEPARTMENT OF THE REGIONAL PUBLIC HEALTH INSTITUTE (NZZJZ PGŽ) we hereby inform you that the test can be done on the island of Rab, where employees of the Rab branch of the Public Health Institute will conduct COVID-19 drive-in tests on personal request. ...
Cullinan Oncology, LLC and The Wistar Institute today announced an agreement to accelerate the development of VK-2019, a novel EBNA1 (Epstein-Barr Nuclear Antigen 1) inhibitor discovered by The Wistar Institute. VK-2019 will be developed by Cullinan Apollo, a company formed and...
BEOOGD GEBRUIK SARS-CoV-2 NP IgG ELISA-kit is een enzymgebonden immunosorbentassay (ELISA) voor de detectie en kwalitatieve meting van IgG-klasse-antilichamen tegen het nucleocapside-eiwit (NP) van het SARS-CoV-2-virus in menselijk bloed. Dit product is bedoeld voor de diagnose van coronavirusziekte 2019 (COVID-19). Dit product is uitsluitend bedoeld voor gebruik door professionele personen. OVERZICHT In december 2019 werd […]. ...
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Japanese encephalitis (JE) is a serious zoonosis caused by the Japanese encephalitis virus (JEV) which is a mosquito-borne pathogen of the family Flavivirus. However, the application of several developed laboratory methods for the detection of JEV antigens or antibodies are limited by their requirements of laboratory operations, skilled technicians and special facilities. To develop a method for detecting JEV antigen in swine, human, mosquito and other clinical specimens specifically, conveniently and effectively, an antigen capture enzyme-linked immunosorbent assay (ELISA) was established in this study. Sensitivity, specificity, repeatability and stability of the developed method were evaluated, and 60 clinical samples were tested in this study. The results demonstrated that the antigen capture ELISA was capable in detecting JEV antigen with high sensitivity and specificity compared with conventional methods. 14 samples showed the positive result with coincidence rate of 70%, and 46 displayed negative
Herpes simplex viruses (HSV) are pathogenic. With the emergence of drug-resistant strains of HSV, new antiviral agents, especially those with different modes of action, are urgently needed. Prunella vulgaris L. (Labiatae), a perennial plant commonly found in China and Europe, has long been used as a …
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Summary Foot-and-mouth disease virus (FMDV) A22 Iraq 24/64 adapted to grow in BHK monolayer cells induced antibodies which neutralized many isolates belonging to the A serotype. Plaque-purified virus isolated from this stock also induced broadly reactive antibodies, showing that this property is not due to the combined response to a mixture of variants in the original stock virus. However, viruses obtained by passage in suspension BHK cells of either the monolayer cell-adapted virus or a virus cloned from this stock resulted in the selection of virus which induced antibodies with highly specific neutralizing activity. In addition to their antigenic properties the monolayer and suspension cell-adapted viruses could be distinguished by plaque morphology, tendency to aggregate and ability to attach to BHK cells. Monoclonal antibodies (MAbs) induced with the plaque-purified monolayer-adapted virus had neutralizing activity almost as broad as polyclonal serum, showing that this property can be represented by
This topic contains 47 study abstracts on Epstein-Barr Virus Infections indicating that the following substances may be helpful: Curcumin, Licorice, and Turmeric
Recombinant proteins of Respiratory Syncytial Virus antigen with high purity and low endotoxin are all produced in house by Sino Biological.
Comparison of washed nasopharyngeal cells and whole nasal secretions for detection of respiratory syncytial virus antigens by enzyme-linked immunosorbent assay ...
Nevertheless, up to now, this passive immune technique has not been carried out as a therapy for RVA-linked diarrhea. Although Ab muscles can have higher
Bovine Serum Albumin (BSA) Antigen Capture ELISA - Photometric, 5 x 96 Tests. |p|The advent of biological products produced using animal components has created a need to ensure their removal prior to use.
When the mono spot test is negative, the optimal combination of EBV serologic testing consists of the antibody titration of four markers: IgM and IgG to the viral capsid antigen, IgM to the early antigen, and antibody to EBNA ...
Antigen test reliably and quickly triages people suspected of SARS-CoV-2, with results ready in 15 minutes, allowing informed treatment decisionsAntigen...
View Notes - Immunogenes or Antigens from STEP 1 at Montgomery College. ‫بسم اللة الرحمن‬ ‫الرحيم‬ Immunogens Or Antigens Immunogens Or Antigens Immunogens or
Viral Antigen ID: Performed on specimens obtained via nasopharyngeal or nasal swabs.. The test methodology is a well-established, widely used Fluorescent Immunoassay (FIA), provided under FDA-granted EUA for the detection and identification of specific protein structures associated with SARS-CoV-2, namely: the spike protein, and the nucleocapsid protein (both known as viral antigens), is highly specific for confirming COVID-19. The FDA has authorized one. (1) testing system EUA in determining the presence of SARS-Co-V-2 Antigen. That test, ...
There are a lot of coronavirus tests that can determine whether you are infected with the coronavirus or not, and they also help find out if the virus has
There are no specific protocols for Recombinant Cytomegalovirus pp28 protein (ab43038). Please download our general protocols booklet
The basic components of the diagnostic test systems are antigens and specific antibodies. The main objective of developing express tests for the diagnosis of bovine leukemia virus (BLV) is to obtain a virus antigen drug, which is very time-consuming to prepare. This problem can be solved by producing anti-idiotype antibodies that have a chemical structure identical to that of the viral antigen and does not require large expenditures to manufacture [1, 2 ...
Free resource for searching and exporting immune epitopes. Includes more than 95% of all published infectious disease, allergy, autoimmune, and transplant epitope data.
Antigen processing is a process that.... Antigen processing is a biological process that prepares antigens for presentation to special cells of the immune system called T lymphocytes.
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The Rapid Antigen Test is easy to use and takes place as a smear in the anterior nasal cavity to determine the detection of antigens.
Discussion. In our study, we found 2 age peaks in the incidence of primary infection by EBV before adolescence: 2-4 years and 7 years. Historically, the literature has described a higher incidence in adolescence.2 In our sample, up to 64% of patients with a serologic diagnosis of primary infection by EBV were aged less than 10 years, probably because older children have a more florid presentation, which in many cases leads to a clinical diagnosis without the ordering of confirmatory laboratory tests that was the basis of our study.14-16. Despite the higher frequency in younger children, our study found a predominance of the typical presentation (65 cases). In the group of patients with typical features, 13 only had 2 symptoms and 52 more than 2 symptoms. However, in many instances the third symptom belonged to the composite variables other symptoms, which encompassed clinical manifestations that are not specifically part of mononucleosis syndromes. This approach to the classification of ...
MACS GMP PepTivator EBV EBNA-1 is a peptide pool that consists mainly of 15-mer peptides with 11 amino acids overlap. It has been developed for efficient in vitro stimulation and subsequent isolation of EBV EBNA-1-specific CD4+ and CD8+ T cells. - Liechtenstein
We have two kinds of cells in our body which are used to fight diseases: B cells and T cells. Whenever an antigen attacks our body for the first time, these cells create a code to fight those antigens. Once the code is created, next time the antigen attacks, these cells are able to fight the antigen very fast as they already have the code created for that specific antigen ...
Congenital human cytomegalovirus (HCMV) infection is the major cause of birth defects and a precise definition of the HCMV-specific T-cell response in primary ...
Learn the difference between antigen and pathogen . Explore how these two vary from each other and discover if they have any similarities only on BYJUS Biology.
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