PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Molecular Oncology Program, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL 33612, USA. Signal transducers and activators of transcription (STATs) were originally identified as key components of signaling pathways involved in mediating responses to IFNs. Previous studies showed that the Src oncoprotein constitutively activates one STAT family member, Stat3. In this study, we investigated STAT activation in a panel of rodent fibroblast cell lines stably transformed by diverse viral oncoproteins. Using a temperature-sensitive mutant of v-Src, we determined that Stat3 is activated within 15 min of shift from nonpermissive to permissive temperature for cell transformation. This finding indicates that v-Src tyrosine kinase activity is required for Stat3 activation and suggests that Stat3 is proximal to signaling initiated by Src. In addition, Stat3 activation is induced by another nonreceptor tyrosine kinase, v-Fps; by polyoma virus middle T antigen, which activates Src family kinases; ...
848 Recapitulating human disease in a genetically engineered mouse (GEM) has become an important source for understanding human tumorigenesis and evaluating novel therapeutic approaches. Furthermore, molecular expression profiling of tumors has increased our understanding of the molecular diversity of disease and classification of tumors. Since many cancers involve mutations in the tumor suppressor genes p53 and the retinoblastoma (Rb), which deregulate the cell cycle, apoptotic mechanisms, and genomic stability, relevant GEM models which functionally inactive these genes through the expression of the Simian Virus 40 T-antigen (Tag) have been developed for human prostate, breast and lung carcinomas. Expression profiles from three types of GEM tumors have been analyzed to identify both common and tumor-specific gene expression signatures for these three types of epithelial tumors. Mammary tumors were derived from C3(1)/Tag transgenic mice, prostate tumors came from probasin/Tag (TRAMP) mice, and ...
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FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Recombinant human EGFR (L792H L858R) (668-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Species :Human Tag :GST tag Expression System:Sf9 insect cells using baculovirus Sequence :668-end (L792H L858R) Genbank Number :NM_005228 S
FOR BULK ORDER REQUESTS PLEASE CONTACT US Description :Recombinant human MAST2 protein was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Species :Human Tag :GST tag Expression System:Sf9 insect cells using baculovirus Sequence :1-792 Genbank Number :BC015816 Purity :Sample Purity Data. For s
Cells were derived by infection of hTERT-HPNE E6/E7 cells (ATCC CRL-4036) with retroviral vector (pBabeZeo) carrying the SV40 small t antigen
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Abstract: Abstract Polyomaviruses have provided many insights into control of cell physiology. Studies of their tumor antigens (Tags) have led to appreciation of the role of tyrosine phosphorylation, PI3K and p53 in oncogenic transformation. This work explores signal pathways regulated by polyomavirus small T antigen (PyST) that control differentiation and regulate cell survival in fat, muscle and... read more bone models. Comparisons of murine polyomavirus ST (PyST) to monkey polyomavirus SV40 (SV40 ST) have been especially useful in parsing out the mechanisms involved. This work also makes use of PyST mutants defective in specific interactions. Of the many PyST functions, we particularly illustrate the importance of phosphatase 2A (PP2A) for PyST to regulate differentiation. PP2A regulates almost all cell signaling pathways. The holoenzyme consists of a catalytic C subunit and one of many regulatory B subunits bound to an A scaffolding subunit. Of more than 80 PP2A isoforms, 10% use Abeta as a ...
1039 Thrombospondin-1 (TSP-1) has been shown to be an effective anti-angiogenic and anti-tumorigenic protein in vitro and in many xenograft mouse models. These models are limited in the information they provide because they dont recapitulate the natural progression of the tumor at the correct body site. We were interested to know how TSP-1 affects breast cancer progression in vivo. The approach we decided to take was to develop a transgenic mouse model for breast cancer in order to enable us to study tumor progression at different stages (ie. initiation to metastasis). We crossed TSP-1 +/+ and TSP-1 -/- female mice with male polyomavirus middle T Antigen (PyT)/mouse mammary tumor virus (MMTV) transgenic mice to generate progeny that are PyT +/-, TSP-1 +/+ and PyT +/-, TSP-1-/-. At 60 days of age when tumors are becoming palpable, we found that tumors in the PyT +/-, TSP-1 -/- mice are 35% larger than tumors found in the PyT +/-, TSP-1+/+. In our preliminary analysis on blood vessel density, ...
The oligomers formed by a mutant nonkaryophilic large T antigen of simian virus 40, which lacks residues 110 through 152 of normal large T antigen and transforms only established cells (L. Fischer-Fantuzzi and C. Vesco, Proc. Natl. Acad. Sci. USA 82:1891-1895, 1985), were found to consist predominantly of dimers. Anti-p53 antibodies precipitated 14 to 16S complexes containing the mutant nonkaryophilic large T antigen and p53 from extracts of transformed cells, and anti-p53 indirect immunofluorescence stained these cells in the cytoplasm. ...
Buy our Recombinant Simian Virus 40 (SV40) SV40 Large T Antigen protein. Ab82118 is a full length protein produced in Baculovirus and has been validated in…
Cerebral cortical development requires orderly transitions between neurogenesis and differentiation. Neurogenesis also results in overproduction of neurons that are selectively targeted for apoptosis. In these experiments, we conditionally immortalized (Almazan and McKay, 1992; Yanai and Obinata, 1994; Taher et al., 1995; Eves et al., 1996) neural precursors from embryonic rat cerebral cortex, to contrast estrogen and neurotrophin regulation of p53-dependent cortical differentiation and death.. The large T antigen promotes mammalian cell cycle by inhibiting checkpoint transcription factors like p53 (for review, see Levine, 1997). Consequently, the Ts/U19 large T antigen mutation permits synchronization of differentiation, by conditionally regulating p53-dependent mechanisms. At the nonpermissive temperature (39°C), large T antigen expression ceases and substantial cell death occurs, that is partly caused by apoptosis. At this temperature, we also observed induction of pp53 and p53-dependent ...
TY - JOUR. T1 - Immortalization of subpopulations of respiratory epithelial cells from transgenic mice bearing SV40 large T antigen. AU - Ikeda, K.. AU - Clark, J. C.. AU - Bachurski, C. J.. AU - Wikenheiser, K. A.. AU - Cuppoletti, J.. AU - Mohanti, S.. AU - Morris, R. E.. AU - Whitsett, J. A.. PY - 1994. Y1 - 1994. UR - http://www.scopus.com/inward/record.url?scp=0028041963&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0028041963&partnerID=8YFLogxK. M3 - Article. AN - SCOPUS:0028041963. VL - 267. JO - American Journal of Physiology - Heart and Circulatory Physiology. JF - American Journal of Physiology - Heart and Circulatory Physiology. SN - 0363-6135. IS - 3 part 1. ER - ...
TY - JOUR. T1 - Further characterisation of the complex containing middle T antigen and pp60.. AU - Courtneidge, Sara. PY - 1989. Y1 - 1989. UR - http://www.scopus.com/inward/record.url?scp=0024387934&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0024387934&partnerID=8YFLogxK. M3 - Article. C2 - 2477198. AN - SCOPUS:0024387934. VL - 144. SP - 121. EP - 128. JO - Current Topics in Microbiology and Immunology. JF - Current Topics in Microbiology and Immunology. SN - 0070-217X. ER - ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Deppert, W and Walter, G, "Domains of simian virus 40 large t-antigen exposed on the cell surface." (1982). Subject Strain Bibliography 1982. 3601 ...
Most of the data on which we base our prostate cancer screening practices is indirect and not definitively linked to the decrease in mortality that has been observed.
Cancers that display a specific combination of sugars, called T-antigen, are more likely to spread through the body and kill a patient. However, what regulates the appearance of T-antigen in cancer cells, the set of proteins modified with T-antigen, and the roles the T-antigen and the modified proteins play during metastasis, is not yet understood.
generating the core 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in ...
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Simian virus (SV40) large T antigen, molecular model. This antigen is from the simian vacuolating virus 40 (SV40). Large T antigens play a role in regulating the viral life cycle of the polyomaviridae viruses, such as SV40. SV40 is found in monkeys such as Rhesus monkeys and macaques. Potentially tumour-causing in primates and humans, it is used in laboratory research and in vaccines. - Stock Image C025/1808
The ability of normal mice to mount an SV40 T antigen-specific cytolytic T lymphocytes response when immunized in vivo with splenocytes from the SV40 T antigen transgenic 427-line mice and restimulated in vitro with SV40-transformed fibroblasts, or when immunized with SV40 and restimulated with 427-line splenocytes, was analyzed. Both immunization schemes resulted in an SV40 T antigen-specific immune response, indicating the presence of SV40 T antigen-positive cells in the spleens of these transgenic mice. Normal mice engrafted with skin from 427 donors showed no rejection of the graft. Thus, SV40 T antigen in transgenic 427-line mice is expressed on an undefined cell type in the spleen and acts as a tissue-specific minor histocompatibility antigen.
The simian virus 40 small T-associated 56,000-Mr (56K) and 32K cellular proteins were shown to be closely related to the polyomavirus medium T-associated 61K and 37K cellular proteins as demonstrated by two-dimensional polyacrylamide gel electrophoresis and V8 protease peptide mapping. ...
DELETION OF THE CARBOXY TERMINUS OT SIMIAN VIRUS 40 LARGE T ANTIGEN AFFECTS VIRAL LATE GENE EXPRESSION A Thesis Submitted to the Faculty in partial fulfillment of the requirements for the degree of Doctor of Philosophy Terryl Stacy DARTMOUTH COLLEGE Hanover, New Hampshire March 8,1990 ...
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Looking for online definition of T-antigen in the Medical Dictionary? T-antigen explanation free. What is T-antigen? Meaning of T-antigen medical term. What does T-antigen mean?
Oncogenes of DNA tumor viruses encode proteins that cause cells to divide incessantly, eventually leading to formation of a tumor. These oncoproteins have now been found to antagonize the innate immune response of the cell (link to paper).. Most cells encountered by viruses are not dividing, and hence do not efficiently support viral DNA synthesis. The genomes of adenoviruses, polyomaviruses, and papillomaviruses encode proteins that cause cells to divide. This effect allows for efficient viral replication, because a dividing cell is producing the machinery for DNA synthesis. Under certain conditions, infections by these viruses do not kill cells, yet they continue to divide due to the presence of viral oncoproteins. Such incessant division gives the cells new properties - they are called transformed cells - and they may eventually become a tumor.. These so-called viral oncoproteins include large T antigen (of SV40, a polyomavirus); E6 and E7 (papillomavirus), and E1A (adenovirus). These viral ...
This topic contains 11 study abstracts on Simian virus 40 (SV40) indicating it may contribute to Simian virus 40 (SV40), Mesothelioma, and Cancer Metastasis
Kupffer cells have been isolated from transgenic mice carrying a thermolabile SV40 large tumor antigen under the H2Kb promoter (kind gift of D. Kioussis, NIMR, London). The cells grow with Interferon-gamma at 33oC, at which temperature the promoter is turned on and the SV40T Ag is active. They differentiate at 39oC. These cells are now being characterised: cytokine and NO liberation is stimulated, surface receptors are assessed at the mRNA and protein level, and phagocytosis and uptake of bacterial components are being measured. Results will be compared with the functional characteristics of primary Kupffer cells isolated from normal mice (see also 3R project 73-00) Conclusions and Relevance for 3R ...
in Research in Veterinary Science (2009), 87(1), 123-32. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be ... [more ▼]. In the present study we developed an enzymatic approach (through the use of collagenase and dispase) to isolate bovine intestinal epithelial cells. Using this method, freshly isolated jejunocytes could be distinguished from simultaneously isolated colonocytes, as the jejunocytes specifically exhibited the small intestinal peptidase gene transcript, as well as an active alkaline phosphatase. The transformation of both types of cell suspension was performed by retroviral infection, using reproduction-defective viruses bearing the gene coding for the large T antigen of the leukaemia simian virus (SV40). The success of the transfection was demonstrated by (1) a significant increase in cell passage numbers (52-53 vs. 7 passages ...
Flukes process calibration software helps you manage instrument inventory, schedule calibrations, create tag-specific procedures, store procedures, and run as-found and as-left procedures in the field or on the bench, and print a selection of pre-formatted reports. See the details here.
Hintergrund und Fragestellung: Die Etablierung von humanen Zellinien wurde bisher häufig limitiert durch die in der Regel nach einigen Passagen auftretende Krise der Zellen in Kultur. Zur...
Transgenic mice were generated using a construct that encodes mouse polyoma virus large T antigen, one of three oncogenic products of the "early region" of the polyoma viral genome. Of 16 transgenic families developed, 1 was characterized by a neurologic disorder consisting of constant tremor and recurrent seizures. Morphologic analysis of the central nervous system (CNS) of affected transgenic mice included: classical light and electron microscopic examination; immunohistochemical assessment of the presence and localization of myelin-specific proteins, of the astrocyte marker glial fibrillary acidic protein, of the oligodendrocyte marker galaetosyl cerebro-side, and of large T; double immunolabeling of glial fibrillary acidic protein or galactosyl cerebro-side and large T to identify the CNS cell type in which large T is expressed; and in situ hybridization to study myelin basic protein gene expression. Our results suggest that polyoma large T is expressed in astrocytes, possibly resulting in ...
Marine mammals, such as whales, have a high proportion of body fat and so are susceptible to the accumulation, and associated detrimental health effects, of lipophilic environmental contaminants. Recently, we developed a wild-type cell line from humpback whale fibroblasts (HuWa). Extensive molecular assessments with mammalian wild-type cells are typically constrained by a finite life span, with cells eventually becoming senescent. Thus, the present work explored the possibility of preventing senescence in the HuWa cell line by transfection with plasmids encoding the simian virus large T antigen (SV40T) or telomerase reverse transcriptase (TERT). No stable expression was achieved upon SV40 transfection. Transfection with TERT, on the other hand, activated the expression of telomerase in HuWa cells. At the time of manuscript preparation, the transfected HuWa cells (HuWaTERT) have been stable for at least 59 passages post-transfection. HuWaTERT proliferate rapidly and maintain initial cell ...
Human polyomaviruses (JC virus, BK virus and simian virus 40) are causative agents of some human diseases and, interestingly, are involved in processes of cell transformation and oncogenesis. These viruses need the cell cycle machinery of the host cell to complete their replication; so they evolved mechanisms that can interfere with the growth control of infected cells and force them into DNA replication. The retinoblastoma family of proteins (pRb), which includes pRb/p105, p107 and pRb2/p130, acts as one of the most important regulators of the G1/S transition of the cell cycle. Rb proteins represent an important target for viral oncoproteins. Early viral T antigens can bind all members of the pRb family, promoting the activation of the E2F family of transcription factors, thus inducing the expression of genes required for the entry to the S phase. The interaction between early viral antigens and cell cycle regulators represents an important mechanism through which viruses deregulate cell cycle ...
WU polyomavirus is a recently described polyomavirus found in patients with respiratory infections. Of 2,637 respiratory samples tested in St. Louis, Missouri, 2.7% were positive for WU polyomavirus by PCR, and 71% were coinfected with other respiratory viruses. Persistent human infection with WU polyomavirus is described ...
Using the TCGA data set for malignant melanoma, the patients were segregated according to the presence or absence of the T cell-inflamed gene expression signature in the tumor microenvironment. Transcriptional profiling revealed no differences in the levels of cancer-testis (CT) antigens or differentiation antigens between the hot and the cold tumors. Using exome sequencing of tumor versus germline, a range of 18 to 3001 of non-synonymous mutations was observed in both cohorts. Using the syfpeithi algorithm for HLA-A*0201 patients, a median of 123 mutations having a high immunogenicity score were found in the T cell-inflamed cohort versus 176 in the non-T cell-inflamed. To confirm actual immunogenicity, 321 peptides from hot tumors and 409 peptides from cold tumors have been synthesized. Using a high-throughput T2 binding assay, peptides from both cohorts were found to bind to HLA-A*0201. In vitro priming of T cells using autologous dendritic cells also revealed that peptides from both cohorts ...
Here, we demonstrated the feasibility of a chemical synthetic lethality screen in cultured MEFs using a double-label fluorescence system. The major challenge in the present work has been to identify a replicon that can stably replicate in MEFs while under selection and decay spontaneously at a reasonable rate on removal of selection pressure. Our initial attempt, which employed polyoma virus replicons, failed. These replicons, containing wild-type or mutated enhancer/origin of DNA replication and encoding polyoma large T antigen, were shown previously to be capable of replicating in either somatic mouse cells (16) or pluripotent embryonal mouse cells such as EC cells (17, 18) or mouse ES cells (19), respectively. However, we found out that these polyoma-based replicons could not replicate in MEFs, which are late embryonal mouse cells. In contrast, employment of the EBV-based episomal survival plasmid, previously used in human cells (13), turned out to be suitable for synthetic lethality ...
Resveratrol exhibits a strong cytotoxic activity in cultured cells and has an antiviral action against polyomavirus: potential clinical use. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
This is a clone of the SV40LT-SMC cell line derived from primary abdominal aortic tissue from young rats. The parental line was created by immortalizing the primary cells by transfection with SV40 large T antigen.
not, multiscale data are fluid to Паркет, ламинат, массив, линолеум 2010 and observing or targeting. primarily, following developments mix found to the gespecialiseerd of the notion, and are transported with membrane journals. Паркет, ламинат, массив, линолеум 2010 fluids propose separated to the depth of the diode with large T equations.
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A human polyomavirus was recently discovered in Merkel cell carcinoma (MCC) specimens. The Merkel cell polyomavirus (MCPyV) genome undergoes clonal integration into the host cell chromosomes of MCC tumors and expresses small T antigen and truncated large T antigen. Previous studies have consistently reported that MCPyV can be detected in approximately 80% of all MCC tumors. We sought to increase the sensitivity of detection of MCPyV in MCC by developing antibodies capable of detecting large T antigen by immunohistochemistry. In addition, we expanded the repertoire of quantitative PCR primers specific for MCPyV to improve the detection of viral DNA in MCC. Here we report that a novel monoclonal antibody detected MCPyV large T antigen expression in 56 of 58 (97%) unique MCC tumors. PCR analysis specifically detected viral DNA in all 60 unique MCC tumors tested. We also detected inactivating point substitution mutations of TP53 in the two MCC specimens that lacked large T antigen expression and in ...
Author Summary Viruses are obligate intracellular pathogens, and all of them share one crucial step in their life cycle-the attachment to their host cell via cellular receptors, which are usually proteins or carbohydrates. This step is decisive for the selection of target cells and virus entry. In this study, we investigated murine polyomavirus (MuPyV), which attaches to host gangliosides with its major capsid protein, VP1. We have solved the crystal structures of VP1 in complex with previously known interaction partners as well as with the ganglioside GT1a, which we have identified as a novel functional receptor for MuPyV. Earlier studies have shown that different strains with singular amino acid exchanges in the receptor binding pocket of VP1 display altered pathogenicity and viral spread. Our investigations show that, while these exchanges do not abolish binding or significantly alter interaction modes to our investigated carbohydrates, they have subtle effects on glycan affinity. The combination of
A short sequence of predominantly basic amino acids Pro-Pro-Lys-Lys-Lys-Arg-Lys-Val from SV40 Large T is responsible for the normal nuclear location of the protein. Alteration of Lys-128 to each of six different residues other than Arg renders Large T cytoplasmic, whereas single amino acid changes in the surrounding region impair but do not prevent nuclear accumulation. When transposed to the amino terminus of cytoplasmic Large T species, or Escherichia coli β-galactosidase or of chicken muscle pyruvate kinase, the sequence around Lys-128 of Large T is able to direct the recipient protein to the nucleus. This demonstrates that these amino acids can be sufficient for nuclear location and can act as a nuclear location signal. A computer search of over 2500 proteins reveals that some other nuclear proteins (for example, BK virus Large T, SV40 VP2 and adenovirus 72kDa DNA binding protein) contain very similar basic tracts, but so too do some presumed non-nuclear proteins (for example, poliovirus ...
Expression of Human Neurotrophic Polyomavirus JCV Late Gene Product Agnoprotein in Human Medulloblastoma. Del Valle, Luis; Gordon, Jennifer; Enam, Sahnila; Delbue, Serena; Croul, Sidney; Abraham, Selvajothi; Radhakrishnan, Sujatha; Assimakopoulou, Martha; Katsetos, Christos D.; Khalili, Kamel // JNCI: Journal of the National Cancer Institute;2/20/2002, Vol. 94 Issue 4, p267 Investigates the expression of human neurotrophic polyomavirus JCV agnoprotein gene in human medulloblastoma. Components of JCV genome; Role of polyomavirus JCV in demyelinating disease; Effect of T antigen on the nerve cells. ...
Many humans have antibodies against simian lymphotropic polyomavirus (LPyV), but its DNA has not been found in humans. Identification of human polyomavirus 9 (HPyV9) led us to compare the seroprevalence and cross-reactivity of LPyV and HpyV9. Results could indicate that humans who have antibodies against LPyV are infected by HPyV9.
In this work we report on cytotxicity versus two different cell lines: a normal mouse firbroblast line and tumoral one. The results clearly show that RV can exert a cytotoxic action both against a normal stabilized fibroblast cell line and human tumor cells. The human tumor line seems to be slightly more sensitive to the drug and this recalls results previously obtained in our laboratory with MEX: a partially purified natural mixture [18]. The antiviral activity of resveratrol towards murine polyomavirus infection was also evaluated. The exposure to the drug was carried at a concentration of RV which did not show a significant cytotoxic effect. It is known that resveratrol can exert anti-oxidant and anti-inflammatory activities and, also, it regulates multiple cellular events associated with carcinogenesis: for a relatively recent review see [28]. The cytotoxicity of RV is only apparently paradoxical; as a matter of fact this drug induces cell cycle arrest and stimulates the Reactive Oxygen ...