Fedry J, Forcina J, Legrand P, Pehau-Arnaudet G, Haouz A, Johnson M*, Rey FA*, Krey T*. 2018. Evolutionary diversification of the HAP2 membrane insertion motifs to drive gamete fusion across eukaryotes. PLoS biology 16:e2006357. (M.J., F.A.R. and T.K. contributed equally to this work) Ströh LJ, Nagarathinam K, Krey T. 2018. Conformational Flexibility in the CD81-Binding Site of the Hepatitis C Virus Glycoprotein E2. Frontiers in immunology 9:1396. Vasiliauskaite I, Owsianka AM, England P, Khan AG, Cole S, Bankwitz D, Foung SKH, Pietschmann T, Marcotrigiano J, Rey FA, Patel AH*, Krey T*. 2017. Conformational Flexibility in the Immunoglobulin-Like Domain of the Hepatitis C Virus Glycoprotein E2. mBio 8:e00382-00317. (A.H.P. and T.K. contributed equally to this work) Fedry J, Liu Y, Pehau-Arnaudet G, Pei J, Li W, Tortorici MA, Traincard F, Meola A, Bricogne G, Grishin NV, Snell WJ*, Rey FA*, Krey T*. 2017. The Ancient Gamete Fusogen HAP2 Is a Eukaryotic Class II Fusion Protein. Cell ...
The primary reservoir for hepatitis C virus (HCV) replication is believed to be hepatocytes, which are highly polarized with tight junctions (TJ) separating their basolateral and apical domains. HepG2 cells develop polarity over time, resulting in the formation and remodeling of bile canalicular (BC) structures. HepG2 cells expressing CD81 provide a model system to study the effects of hepatic polarity on HCV infection. We found an inverse association between HepG2-CD81 polarization and HCV pseudoparticle entry. As HepG2 cells polarize, discrete pools of claudin-1 (CLDN1) at the TJ and basal/lateral membranes develop, consistent with the pattern of receptor staining observed in liver tissue. The TJ and nonjunctional pools of CLDN1 show an altered association with CD81 and localization in response to the PKA antagonist Rp-8-Br-cyclic AMPs (cAMPs). Rp-8-Br-cAMPs reduced CLDN1 expression at the basal membrane and inhibited HCV infection, supporting a model where the nonjunctional pools of CLDN1 ...
Meola, Annalisa et al "Structural Flexibility of a Conserved Antigenic Region in Hepatitis C Virus Glycoprotein E2 Recognized by Broadly Neutralizing Antibodies." Journal of Virology 89.4 (2015): 2170-2181. Web. 08 Dec. 2019. ...
|p|CD81 is a 26 kD non-glycosylated member of the tetraspanin superfamily (TM4SF), also known as TAPA-1 (target of an antiproliferative antibody). CD81 is expressed on T and B cells, NK cells, monocytes, dendritic cells, thymocytes, endothelial cells, and fibroblasts. It also has low levels of expre
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v dsk policie zabavila p tadvacetilet mu mu i z m sta Bojsenburg bramborov d lo, kter postavil spolu s kamar dy. Z okna sv ho bytu j m st lel brambory do okol . D lo si vyrobil podle televizn ho po adu, kter ho inspiroval.
TY - JOUR. T1 - Antigenicity of hepatitis C virus envelope proteins expressed in Chinese hamster ovary cells. AU - Inudoh, M.. AU - Nyunoya, H.. AU - Tanaka, T.. AU - Hijikata, M.. AU - Kato, N.. AU - Shimotohno, K.. PY - 1996/12/1. Y1 - 1996/12/1. N2 - A putative second envelope glycoprotein (E2) of hepatitis C virus (HCV) was constitutively produced in a Chinese hamster ovary cell line stably transformed with a plasmid expressing E2 protein under the control of an exogenous promoter and a signal sequence. E2 protein that lacked part of the C-terminal hydrophobic region was glycosylated with high-mannose type oligosaccharides and retained in the cells. On the other hand, E2 protein lacking the entire C-terminal hydrophobic region was glycosylated with complex type oligosaccharides (complex form) and excreted into the culture medium. Immunoreactivity of the high-mannose and complex forms of E2 proteins against sera from HCV infected patients were analyzed. We found that the antigenicity of the ...
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Fingerprint Dive into the research topics where Tapas Hazra is active. These topic labels come from the works of this person. Together they form a unique fingerprint. ...
Disclosed is an electroluminescent device comprising a cathode and an anode; and, located therebetween, a light-emitting layer (LEL) comprising a phosphorescent green-light-emitting material and a host material for the light-emitting material, and in a layer adjacent to the LEL on the anode side, an exciton-blocking layer containing a compound having a hole mobility of at least 1 10−3 cm2V−1s−1 and a triplet energy exceeding that of the green-light-emitting material of the LEL. Such a device provides useful light emission.
The Overlocker Technique Manual: The Complete Guide to Serging and Decorative Stitching de Julia Hincks en Iberlibro.com - ISBN 10: 1782210202 - ISBN 13: 9781782210207 - Search Press Ltd - 2014 - Tapa blanda
Tetraspanins are integral transmembrane proteins organized in microdomains displaying specific and direct interactions with other tetraspanins and molecular partners. Among them, CD81 has been implicated in a variety of physiological and pathological processes. CD81 also plays a crucial role in pathogen entry into host cells, including hepatitis C virus (HCV) entry into hepatocytes. HCV is a major cause of liver cirrhosis and hepatocellular carcinoma. HCV entry into hepatocytes is a complex process that requires the coordinated interaction of viral and host factors for the initiation of infection, including CD81, scavenger receptor BI, claudin-1, occludin, membrane-bound host cell kinases, Niemann-Pick C1 Like 1, Harvey rat sarcoma viral oncogene homolog (HRas), CD63 and transferrin receptor 1. Furthermore, recent data in HCV model systems have demonstrated that targeting critical components of tetraspanins and associated cell membrane proteins open new avenues to prevent and treat viral infection.
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Clone REA716 recognizes the human CD36L1 antigen, an integral membrane protein, also known as SR-BI. CD36L1 is widly expressed, e.g. on macrophages, dendritic cells, adrenocortical cells, adipocytes, and trophoblastic cells. In hepatocytes, CD36L1 acts as a receptor for hepatitis C. It has been reported, that CD36L1 is able to bind HCV envelope glycoprotein E2, participate in entry of HCV pseudotype particles, and modulate HCV infection. Furthermore, CD36L1 is a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, vitamin E, phosphatidylserine, and apoptotic cells. Additional information: Clone REA716 displays negligible binding to Fc receptors. - Canada
Years ago, when I was living in Salamanca Spain, I use to visit a local bar that served the best Tapas. They had one that was a small skewer...
Jimao Tapas e Vinhos: Perfect! - See 1,821 traveler reviews, 942 candid photos, and great deals for Porto, Portugal, at TripAdvisor.
Tapas restaurants come and go. Maybe they call their food small plates instead of tapas, but they still want to plug into the same long-outdated...
Want to become better at yoga? Practice. Its that simple, and that challenging. - Tapas, the Niyama of Discipline - Yoga at BellaOnline
Los camareros amables, el arte religioso, el jamón colgado del techo, los clientes que se sientan o se quedan de pie en un bar... ¡Bienvenido a la vida gastronómica de Sevilla! Esta ciudad es el lugar perfecto para descubrir la cocina de Andalucía, ya que es conocida como la capital mundial de la Tapa.
Essentials of Pharmaceutical Chemistry en Iberlibro.com - ISBN 10: 0853699798 - ISBN 13: 9780853699798 - Pharmaceutical Press - 2012 - Tapa blanda
A better understanding of natural variation in neutralization resistance and fitness of diverse hepatitis C virus (HCV) envelope (E1E2) variants will be critical to guide rational development of an HCV vaccine. This work has been hindered by inadequate genetic diversity in viral panels and by a lack of standardization of HCV entry assays. Neutralization assays generally use lentiviral pseudoparticles expressing HCV envelope proteins (HCVpp) or chimeric full-length viruses that are replication competent in cell culture (HCVcc). There have been few systematic comparisons of specific infectivities of E1E2-matched HCVcc and HCVpp, and to our knowledge, neutralization of E1E2-matched HCVpp and HCVcc has never been compared using a diverse panel of human broadly neutralizing monoclonal antibodies (bNAbs) targeting distinct epitopes. Here, we describe an efficient method for introduction of naturally occurring E1E2 genes into a full-length HCV genome, producing replication-competent chimeric HCVcc. We
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Tapas Teatro Cafe: Tapas and the theater - See 122 traveler reviews, 24 candid photos, and great deals for Baltimore, MD, at TripAdvisor.
S&A Foods , the UK independent food manufacturer, has unveiled a new range of tapas for home consumption. Of Spanish origin, tapas snacks have been growing in popularity in Europe over the last decade, and S&A Foods managing director Perween Warsi believes it is time to facilitate the introduction of ready-made tapas for convenient use at home.
Making Tapa Eastern Pacific Style Te Rangi Hiroa (Peter H. Buck) writes in Arts and Crafts of the Cook Islands that the tapa makers of the time (1944) wrapped the pre-beaten bark in banana leaves and left it to ret for three days. Todays tapa makers on Atiu usually beat the bark right away. When…
Stamataki, Zania and Coates, Stephen and Evans, Matthew J and Wininger, Mark and Crawford, Kevin and Dong, Christine and Fong, Yiu-Lian and Chien, David and Abrignani, Sergio and Balfe, Peter and Rice, Charles M and McKeating, Jane A and Houghton, Michael (2007) Hepatitis C virus envelope glycoprotein immunization of rodents elicits cross-reactive neutralizing antibodies. Vaccine, 25 (45). pp. 7773-84. ISSN 0264-410X. ...
Despite staggering community opposition, Cold Miller has managed to secure a liquor license for the small wine and tapas bar hes planning to open at...
Tanzania Tapas Party, Dar es Salaam, January 30, 2016. The National Bailliage was inaugurated in November 2015 with a Chapitre at the Hyatt Regency. It was a grand affair!In complete contrast, the first event of the New Year was an informal dining event at the
FtsX is a ubiquitous bacterial integral membrane protein involved in cell division that regulates the activity of peptidoglycan (PG) hydrolases. FtsX is representative of a large group of ABC3 superfamily proteins that function as "mechanotransmitters," proteins that relay signals from the inside to the outside of the cell. Here, we present a structural characterization of the large extracellular loop, ECL1, of FtsX from the... ...
Meanwhile, slice the chorizo and sauté in a pan over a medium heat. Chop the chicory endive and the tomato and mix. Once the chorizo is cook add the vinegar in the pan and mix with the rest of the salad. Serve with the squid, the combination of sea and land ingredients is fabulous ...
Lel and Zhou beat the heat Kenyas Martin Lel won the mens race of the 2007 Flora London Marathon in blistering heat
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Title: Investigating the molecular mechanism of COPD in tetraspanin CD9/CD81 DKO mice- a new model for ageing. 6/9 Yuko Tsuchiya. 6/16 Special seminar 15:00 ...
Esto es Hoy en @sensesgastrobar Mañana bailarás toda la noche al ritmo de @labandaswing y no te pierdas las estupendas promociones en tus tragos y tapas favoritas. Ahora activos hasta las 2am! #senses #gastrobar #food #foodie #puntofijo #falcon #venezuela #chef #drinks
During the summer months, our garden offers a delightful alternative to eating inside. It really is the perfect place to enjoy our range of tapas. We can happily cater for parties outside - please call for more information. ...
Britsk spisovatel Anthony Horowitz se proslavil d tskou kni n s ri o detektivovi Alexu Riderovi. Poda ilo se mu z skat pr va na Jamese Bonda od rodiny Iana Flemminga, autora cel postavy. Jeho prvn bondovsk rom n se rodin tak l bil, e Horowitzovi umo n ud lat n co, co Ian Flemming zam lel, ale u nestihl - vymyslet pln za tky agenta s k dov m ozna en m 007.
Michal Ulom mu , kter loni v kv tnu brut ln napadl ma etou sv ho n kdej ho psychiatra Karla Hynka - pom lel na sv j in nejm n rok dop edu. Docenta beru sebou do pekla, napsal si do den ku v b eznu 2002. Jeho p b h, o n m MF DNES z skala detailn informace, je p b hem ne astn ho mu e.
The results of this study show that the amount of mobile receptor and the speed at which it diffuses varies according to its location within the cell. CD81 and claudin-1 are expressed equally in the filopodia and plasma membrane, whereas SR-BI is expressed at lower levels in the filopodia compared to the plasma membrane. We show that addition of both sE2 and sE1E2 has varying affects on both the speed and mobility of CD81 and claudin-1 and that the majority of significant effects observed for claudin-1 are observed at areas of potential cell contact. Finally, we demonstrate that addition of ITX5061 affects the diffusion coefficient of CD81 and CLDN-1 and the amount of mobile SR-BI. Furthermore, the effects on SR-BI are limited to areas of cell contact or exploratory regions. In summary, we present data which we hope will further current knowledge of the activity of these receptors in relation to their role in HCV infection ...
Despite extensive research, many details about the structure and functions of hepatitis C virus (HCV) glycoproteins E1 and E2 are not fully understood, and their crystal structure remains to be determined. We applied linker-scanning mutagenesis to generate a panel of 34 mutants, each containing an insertion of 5 aa at a random position within the E1E2 sequence. The mutated glycoproteins were analysed by using a range of assays to identify regions critical for maintaining protein conformation, E1E2 complex assembly, CD81 receptor binding, membrane fusion and infectivity. The results, while supporting previously published data, provide several interesting new findings. Firstly, insertion at amino acid 587 or 596 reduced E1E2 heterodimerization without affecting reactivity with some conformation-sensitive mAbs or with CD81, thus implicating these residues in glycoprotein assembly. Secondly, insertions within a conserved region of E2, between amino acid residues 611 and 631, severely disrupted protein
A Meola, A Sbardellati, B Bruni Ercole, M Cerretani, M Pezzanera, A Ceccacci, A Vitelli, S Levy, A Nicosia, C Traboni, J McKeating, E Scarselli
Human PCSK9 is known to enhance the degradation of membrane-bound receptors such as the hepatocyte low-density lipoprotein receptor (LDLR), ApoER2, and very low-density lipoprotein receptor. Because the LDLR is suspected to be involved in hepatitis C virus (HCV) entry, we also tested whether PCSK9 can affect the levels of CD81, a major HCV receptor. Interestingly, stable expression of PCSK9 or a more active membrane-bound form of the protein (PCSK9-ACE2) resulted in a marked reduction in CD81 and LDLR expression. Therefore, we analyzed the antiviral effect of PCSK9 in vitro using the HCV genotype 2a (JFH1) virus. The results clearly demonstrated that cells expressing PCSK9 or PCSK9-ACE2, but not the ACE2 control protein, were resistant to HCV infection. Furthermore, addition of purified soluble PCSK9 to cell culture supernatant impeded HCV infection in a dose-dependent manner. As expected, HuH7 cells expressing PCSK9-ACE2 were also resistant to infection by HCV pseudoparticles. In addition, we ...
In order to elucidate how Hepatitis C Virus (HCV) interacts with polarized hepatocytes in vivo and how HCV-induced alterations in cellular function contribute to HCV-associated liver disease, a more physiologically relevant hepatocyte culture model is needed. As such, NASA-engineered three-dimensional (3-D) rotating wall vessel (RWV) bioreactors were used in effort to promote differentiation of HCV-permissive Huh7 hepatoma cells. When cultured in the RWV, Huh7 cells became morphologically and transcriptionally distinct from more standard Huh7 two-dimensional (2-D) monolayers. Specifically, RWV-cultured Huh7 cells formed complex, multilayered 3-D aggregates in which Phase I and Phase II xenobiotic drug metabolism genes, as well as hepatocyte-specific transcripts (HNF4α, Albumin, TTR and α1AT), were upregulated compared to 2-D cultured Huh7 cells. Immunofluorescence analysis revealed that these HCV-permissive 3-D cultured Huh7 cells were more polarized than their 2D counterparts with the expression of
Koh Thai Tapas is an award winning restaurant brand, launched in 2009 by Lennox, along with two other business partners, Nick Billingham and James Hampton. Frustrated by the restaurant scene which, according to Lennox, seemed to either follow the pattern of great food delivered with a stuffy fine dining experience or great ambience accompanied by mediocre food quality, the trio set about creating the Complete Dining Experience (CDE). This, says Lennox, is the core driving factor behind the strategy of the business.
OFFALLY DELICIOUS . . #offal #food #the6ix #toronto #restaurant #sake #hospitality #cooking #intestines #pork #horumon #tapas #izakaya #guu # ...
Search for information about Dr. Maitra Tapas, Urologist in Darjeeling, West Bengal and also get directions from an interactive map. Medindia has listing of over 207,000 doctors.
Todos los productos se entregan listos para su uso. Cada artículo incluye su Protocolo de Control de Calidad o Certificado de lote único de trazabilidad el cual indica la duración del producto y condiciones de almacenamiento. Cada unidad está rotulada con serie y vencimiento en forma individual. El uso de estos productos representa una gran economía, seguridad y comodidad.. Placas de Petri: Desechables, plásticas, de óptima calidad y transparencia de aproximadamente 14, 10 y 5 cm de diámetro aproximadamente. Simples, dobles (2 medios), triples (3 medios) y con una gran variedad de medios de cultivo, selectivos y suplementados. Selladas individualmente en cajas de 10 unidades.. Tubos: De diversas medidas, de plástico o vidrio según el producto. Tubos de vidrio de; 75 mm (khan), 12 mm y 16 mm con tapón con doble click, y tubos de fondo plano de 100 mm tapa rosca. Tubos plástico con faldon: de 10 ml y 15 ml con tapa rosca.. Hemocultivos: En frascos de vidrio, tapón de goma y tapa ...
Shop Probable tetraspanin ELISA Kit, Recombinant Protein and Probable tetraspanin Antibody at MyBioSource. Custom ELISA Kit, Recombinant Protein and Antibody are available.
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Whats been your worst job interview? An interview for a customer service role. The interviewer seemed really panicked and uncomfortable. To this day I have no idea why, but I spent the whole hour worrying that I had caused it somehow ...
Moving into summer, the yoga practice should shift towards containment and purification of the expansive, creative spring energies of April and May. In June, berries take the place of blossoms and the trees send their energy to be stored in sweet fruit. The bodys internal furnace, shifting to accommodate sunshine and longer days, must be stoked to create energy for summer projects.. In some ways, June is the fullest time of year. The earth is nourished, radiant and pregnant with possibility. This is a time to take a moment and survey the work youve done on your asanas this spring, add up the lessons and look at the whole picture. Does the tree have roots deep enough to bear the summer harvest? Is there a major leak or blockage in the system? Does a poses anatomy support its physiology?. The asana practice purifies the body with heat (tapas). The torso, or "kumba" is considered a container for tapas. With three main bandhas, loosely translated to energetic locks or "valves," the kumba can leak ...