The role of the calcium- and phospholipid-binding protein annexin I (ANXA1) in cell cycle regulation has been investigated in estrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MB-231 breast tumor cell lines. In MCF-7 cells, ANXA1-targeting small interfering RNA (siRNA) reduced ANXA1 mRNA and protein levels and attenuated cell proliferation induced by FCS, estradiol, or epidermal growth factor. Well-characterized agonists for the known ANXA1 receptor, FPR2, including the ANXA1 N-terminal proteolytic product ANXA1(2-26), lipoxin A(4) (LXA(4)), and the synthetic peptide, Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm), stimulated proliferation of MCF-7 and MDA-MB-231 cells that was attenuated by incubation with FPR2 antagonists WRW(4) (1 μM) or Boc2 (100 nM) or by siRNA against FPR2. FCS-induced mitogenic responses were attenuated by each of the FPR antagonists and by siRNA against FPR2 and, to a lesser extent, FPR1. LXA(4) increased phosphorylation of Akt, p70(S6K) but not ERK1/2. Increases in cyclin ...

Mouse anti Human Annexin A3 antibody, clone 4F1 recognizes human annexin A3, also known as Annexin III, Inositol 1,2-cyclic phosphate 2-ph

TY - GEN. T1 - Neurotrophic roles of the beta-thymosins in the development and regeneration of the nervous system. AU - Sun, Woong. AU - Kim, Hyun. PY - 2007/9/1. Y1 - 2007/9/1. N2 - Beta-thymosins (Tβs) are polypeptides abundant in the cytosol, nucleus, and extracellular space of many cell types. In the nervous system, the expression of Tβs is regulated during the development of the central nervous system and following neuronal insults in cell-type and brain-region dependent manners, which may be related to the function of Tβs in the growth and regeneration of the nervous system. Supporting such a proposition, overexpression of Tβs in neurons has been shown to modify the axonal branches in vivo and neurite branches in vitro. These neurite-modifying functions have been suggested to be due to the activity of Tβs to bind actin. In addition, we recently observed that Tβs suppressed the apoptotic neuronal death in chick embryos, and these functions might be mediated by the extracellularly ...

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Our list of cell biology-related antibodies has been created for your convenience. Click on any target for more information and ordering instructions! GST IKK alpha P38 MAPK Caspase 8 mTOR/FRAP ERK1 TARGET AIF AKT1/3 ALDH1 Annexin VI ARC/Arg3.1 ATF4/CREB-2 ATP1b2/Na+K+ATPase Bax Bax(Ser184) Beclin 1 Beta Actin Beta cat

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The Papal conclave of August 1978, the first of the two conclaves held in the year 1978, was convoked after the death of Pope Paul VI on August 6 at Castel Gandolfo. After the cardinal electors assembled in Rome, the conclave to elect Pauls successor began on August 25 and ended the next day...

CHAPTER VI IMPACT OF PRIORITY SECTOR LENDING 6.1 PRINCIPAL FACTORS THAT HAVE DIRECT IMPACT ON PRIORITY SECTOR LENDING 6.2 ASSOCIATION BETWEEN THE PROFILE VARIABLES AND IMPACT OF PRIORITY SECTOR CREDIT

Mouse Monoclonal Anti-ANXA1 Antibody against Human annexin A1. Validated for Immunofluorescence, Immunohistochemistry and Western Blot

In our hands, the trypsin actually reduced the Annexin staining presumably by stripping off all the phosphotidyl-serine on the surface along with the surface proteins (adhesion molecules). The only time we have seen enhanced annexin is when the cells suffer enough trauma in harvesting that the membrane is not intact and the Annexin gets inside the cell and attaches to the ps on the inner membrane. Julie At 02:11 PM 8/18/98, you wrote: , ,I have a client who is staining with Annexin-FITC. They are looking at ,cell lines and are using trypsin to get the cells off the flasks. We are ,seeing what we think is an exagerated Annexin response. Does anyone out ,there have any feedback on how trypsin affects Annexin staining? Any ,experience and or information about this will be greatly appreciated. , Thanks in advance, , Joan Kalnitsky ,Joan Kalnitsky ,Flow Cytometry Laboratory ,VMRCVM ,(540) 231-4115 ,FAX 540-231-7367 , , It is better to serve than to receive , B. Borg , , , , , ...

TY - JOUR. T1 - Annexin A2. T2 - Its molecular regulation and cellular expression in cancer development. AU - Wang, Chi Yun. AU - Lin, Chiou Feng. PY - 2014. Y1 - 2014. N2 - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 affects its cellular localization and function. However, posttranslational modification and protease-mediated N-terminal cleavage also play critical roles in regulating ANXA2. ANXA2 expression levels vary among different types of cancers. With some cancers, ANXA2 can be used for the detection and diagnosis of cancer and for monitoring cancer progression. ANXA2 is also required for drug-resistance. This review discusses the feasibility of ANXA2 which is active in cancer development and can be a therapeutic target in cancer management.. AB - Annexin A2 (ANXA2) orchestrates multiple biologic processes and clinical associations, especially in cancer progression. The structure of ANXA2 ...

1HVD: Structural and electrophysiological analysis of annexin V mutants. Mutagenesis of human annexin V, an in vitro voltage-gated calcium channel, provides information about the structural features of the ion pathway, the voltage sensor and the ion selectivity filter

This graph shows the total number of publications written about Annexin A3 by people in this website by year, and whether Annexin A3 was a major or minor topic of these publications ...

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.

ATP- and membrane-binding protein that probably controls membrane reorganization/tubulation upon ATP hydrolysis. Plays a role in early endosomal transport.

Gentaur molecular products has all kinds of products like :search , Biotium \ ANNEXIN V CF633 CONJUGATE \ 29008 for more molecular products just contact us

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Annexin A11 Antibody 10479-2-AP has been identified with IF, IHC, IP, WB, ELISA. 10479-2-AP detected 56-60 kDa band in HeLa cells with 1:500-1:2000 dilution...

购买我们的重组人Annexin A3蛋白。Ab92929为全长蛋白，在大肠杆菌中生产并经过Western blot, SDS-PAGE实验验证。Abcam提供免费的实验方案，操作技巧及专业的支持。

Annexin A2兔多克隆抗体(ab90643)可与人样本反应并经WB, IHC实验严格验证。中国75%以上现货，所有产品均提供质保服务，可通过电话、电邮或微信获得本地专属技术支持。

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Annexin A1 is an intracellular calcium/phospholipid-binding protein that is involved in membrane organization and the regulation of the immune system. It has been attributed an anti-inflammatory role at various control levels, and recently we could show that annexin A1 externalization during secondary necrosis provides an important fail-safe mechanism counteracting inflammatory responses when the timely clearance of apoptotic cells has failed. As such, annexin A1 promotes the engulfment of dying cells and dampens the postphagocytic production of proinflammatory cytokines. In our current follow-up study, we report that exposure of annexin A1 during secondary necrosis coincided with proteolytic processing within its unique N-terminal domain by ADAM10. Most importantly, we demonstrate that the released peptide and culture supernatants of secondary necrotic, annexin A1-externalizing cells induced chemoattraction of monocytes, which was clearly reduced in annexin A1- or ADAM10-knockdown cells. Thus, ...

Annexin A2 is a calcium-dependent, phospholipid-binding protein found on many cell types. It consists of a short hydrophobic tail (Ser2-Asn32), which dictates its function, and a core domain (Phe33-Asp339), which is involved in phospholipid binding. Annexin A2 has been implicated in a number of biochemical processes, including cell proliferation, foetal immune tolerance, ion-channel activation, cell-cell interactions and the bridging of membranes. Annexin A2 is reported to be a powerful activator of plasminogen and, therefore, is implicated in many normal and pathological processes such as haemostasis and metastasis. Myeloid cell lines are used, extensively, to study many aspects of cellular proliferation, differentiation and function. In the present study, we have used flow cytometry and real-time PCR to investigate the role of annexin A2 expression in the proliferation and differentiation of a number of myeloid cell lines. The results demonstrated that annexin A2 expression was affected when ...

Early in the apoptosis process, phosphatidylserine (PS) becomes exposed on the cell surface by flipping from the inner to outer leaflet of the cytoplasmic membrane. This event is thought to be important for macrophage recognition of cells undergoing apoptosis, thus allowing the cells to be rapidly phagocytosed. The binding of Annexin V to phosphatidylserine is calcium-dependent, reversible, but very tight, with a Kd of approximately 5 x 10-10 M. At low PS concentration, a binding ratio of 8 annexins to one PS has been reported. These features make Annexin V conjugates ideal for identifying membrane changes associated with apoptosis, by using either flow cytometric or in situ labeling methods. TACS® Annexin V kits are supplied with an Annexin V conjugate, an optimized binding buffer and propidium iodide. Cells are harvested and washed, then incubated with an Annexin V conjugate in binding buffer, for 15 minutes at room temperature prior to direct analysis. Alternatively, a secondary streptavidin ...

Invitrogen™ eBioscience™ Annexin V, Recombinant Protein Unlabeled; 300μg Invitrogen™ eBioscience™ Annexin V, Recombinant Protein...

Annexin V Binding Buffer - Annexin V Binding Buffer has been formulated for flow cytometric labeling of apoptotic cells with Annexin V reagents.  The buffer is provided as a ready-to-use solution..

The Annexin family of calcium-binding proteins is composed of al least thirteen mammalian genes (Annexin A1-13). These proteins are characterized by a conserved core domain which binds to phospholipids in a Ca2+-dependent manner and a unique amino terminal region which may confer binding specificity. The Annexin family

|p|Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry

With Annexin V Apoptosis Assay, detect one of the earliest events in apoptosis-the externalization of phosphatidylserine (PS)-in living cells

With Annexin V Apoptosis Assay, detect one of the earliest events in apoptosis-the externalization of phosphatidylserine (PS)-in living cells

Annexin V FITC Conjugate from human placenta; Synonyms: Calphosbindin I,Lipocortin V,Annexin V,PAP-1; find Sigma-Aldrich-A9210 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich

Buy ANXA2 recombinant protein, Annexin A2 Recombinant Protein-NP_001002857 (MBS203687) product datasheet at MyBioSource, Recombinant Proteins. Application: SDS-PAGE

Bachem offers H-3196 Annexin A1 (1-25) (dephosphorylated) (human) for your research. Find all specific details here. Find product specific information including available pack sizes, CAS, detailed description and references here.

Escherichia coli hõlmab väga suurt bakterite populatsiooni, millel on väga kõrge geneetiline ja fenotüübiline mitmekesisus. E. coli isolaatide ja temaga suguluses olevate bakterite genoomide sekveneerimine on andnud alust kaaluda taksonoomilist ümberpaigutamist, kuid seda pole tehtud tema meditsiinilise olulisuse tõttu.[10] Escherichia coli on geneetiliselt kõige kirjum bakteriliik, kuna ainult 20% genoomist on ühine kõikidele tüvedele.[11] Evolutsiooniliselt peaksid Shigella perekonna liikmed klassifitseeruma E. coli tüvedena, kuid nad on märgitud hoopiski parafüleetilise rühmana.[12] Kuid seevastu E. coli tüvi K-12, keda kasutatakse sageli rekombinantse DNA tegemiseks, peaks kuuluma hoopis teise perekonda, kuna on piisavalt erinev tüüptüvest. Tüvi on liigi alarühm, kes on oma omadustelt väga ainulaadne ja erineb seega oluliselt teistest tüvedest. Need erinevused on sageli tuvastatavad ainult molekulaarsel tasemel, kuid sellegipoolest võivad nad põhjustada muutusi ...

Richter-Helm BioLogics will help Athera Biotechnologies with development and manufacturing of its cardiovascular product. The recombinant protein Annexin A5 is intended for the treatment of patients with acute coronary syndrome who are at risk for myocardial infarction. ...

Synonyms: Uteroglobin, Clara cell 17 kDa protein, Clara cell phospholipid-binding protein, CCPBP, Clara cells 10 kDa secretory protein, CC10, PCB-binding protein, Secretoglobin family 1A member 1, Scgb1a1, Ugb, Utg, UG, CC16, CCSP, PCB-BP. ...

Complete information for ANXA1 gene (Protein Coding), Annexin A1, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Complete information for ANXA5 gene (Protein Coding), Annexin A5, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidyl-serine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 10 minutes. Detection can be analyzed by flow cytometry or by fluorescence microscopy. |p>|br />|br />‧ Detection method- Flow cytometry (Ex = 543 nm; Em = 570 nm) and fluorescence microscopy |br>|br />‧ Sample type- Living cells (suspension and adherant)|br>|br />‧ Species reactivity- Mammalian |br />|br />|br />|p>|b>Features and Benefits|/b>|br>|br />‧ Simple one step staining procedure in 10 minutes|br>|br />‧ Fast and convenient|br>|br />‧ Cy3 shows brighter red fluorescence|br />|br />|br />|br />|p>|b>Kit Contents:|/b>|br>|br />Annexin V-Cy3|br>|br />1X Binding Buffer, Annexin V

Invitrogen™ eBioscience™ Annexin V Apoptosis Detection Kit PerCP-eFluor™ 710 200 tests Invitrogen™ eBioscience™ Annexin V...

TransDetect® Annexin V-EGFP/PI Cell Apoptosis Detection Kit,Cell Detection,Cell Culture and Detection,Products,Beijing TransGen Biotech Co.Ltd,OverviewContents& storageCitations & referencesRelated ImagesDownloadOverviewDescriptionThe Annexin V

Annexin 1 is an anti-inflammatory protein that plays a key role in innate immunity by modulating the activation of several types of cells, including neutrophils. Here we have developed a cleavage assay using tagged annexin 1 and observed marked activity in the membrane fraction of activated neutrophils. A combination of inhibitors, transfected cells, and proteomic analyses allowed us to identify proteinase 3 as the main enzyme responsible for this cleavage in the N terminus region of the protein, at least in the context of neutrophil activation. Because annexin 1 is an important endogenous anti-inflammatory mediator, blocking its cleavage by proteinase 3 would augment its homeostatic pro-resolving actions and could represent an opportunity for innovative anti-inflammatory drug discovery.. ...

Adsorption of Chromium on dye contaminated soil has been studied using batch adsorption technique. This study is carried out to examine adsorption capacity of Chromium on..

Annexin V is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Annexin V is a monomer, binds ATRX and EIF5B, and interacts with hepatitis B virus (HBV ...

During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis., Annexin V-FITC Apoptosis Detection Reagent, GTX14082, Applications: FACS; Flow cytometry/FACS; CrossReactivity:

SCAN HISTORY OWL11_0 2 100 NSINGLE OWL17_1 2 110 NSINGLE OWL18_0 1 115 NSINGLE OWL19_1 1 125 NSINGLE OWL21_1 1 125 NSINGLE OWL26_0 1 125 NSINGLE SPTR37_9f 2 237 NSINGLE INITIAL MOTIF SETS ANNEXIN1 Length of motif = 23 Motif number = 1 Annexin motif I - 1 PCODE ST INT KTKGVDEVTIVNILTNRSNAQRQ LUHU36 46 46 KTKGVDEVTIINILTNRSNEQRQ ANX2_CHICK 46 46 TVKGVDEATIIDILTKRNNAQRQ ANX1_CAVCU 56 56 MVKGVDEATIIDILTKRNNAQRQ LUHU 55 55 KGIGTDEATIIDIVTHRSNAQRQ ANX6_MOUSE 376 376 KGMGTDEETILKILTSRNNAQRQ ANX5_CHICK 29 29 KGIGTNEQAIIDVLTKRSNTQRQ ANX8_HUMAN 35 35 KGFGTDEQEIIDVLVGRSNQQRQ DROANNX 29 29 RGIGTDEKMLISILTERSNAQRQ ANX3_HUMAN 32 32 KGLGTDEDAIINVLAYRSTAQRQ ANX4_BOVIN 27 27 KGFGTDEQAIVDVVANRSNDQRQ HUMSNEXIN 177 177 TAKGVDEATIIDIMTTRTNAQRP ANX1_COLLI 51 51 ANNEXIN2 Length of motif = 17 Motif number = 2 Annexin motif II - 1 PCODE ST INT LKSALSGHLETVILGLL LUHU36 86 17 LKSALSGHLEAVILGLL ANX2_CHICK 86 17 LKKALTGHLEEVVLALL ANX1_CAVCU 96 17 LKKALTGHLEEVVLALL LUHU 95 17 LKSEISGDLARLILGLM ANX6_MOUSE 416 17 ...

Hochwertige Annexin V FITC Kits für Apoptose Detektion mit Flow Cytometry. ✅ Namhafte Top-Hersteller im Vergleich. ✅ Top-Support bei Fragen.

BioAssay record AID 502910 submitted by ChEMBL: Induction of annexin 2-mediated cytoskeleton aggregate accumulation in human HepG2 cells at 4 uM after 2 hrs by confocal laser scanning microscopy.

Spetsiifiliste söötmete alla liigitatakse näiteks sporulatsioonisööde ning indikaatorsöötmed. Sporulatsioonisööde kujutab nälja tingimusi rakkudele. Toitainete puuduses läbivad diploidsed pärmirakud meioosi ning sporuleeruvad (spoor moodustades neli askospooriga eoskoti (eoskott). Diploidsed pagaripärmi rakud sporuleeruvad, kui nad satuvad lämmastiku- ja fermenteeritava süsiniku nälga. Pagaripärmi sporulatsioonisööde sisaldab 10 korda vähem pärmiekstrakti ja 40 korda vähem glükoosi kui rikas sööde. Indikaatorsöötmed võimaldavad kindlaks teha, kas antud tüvi on võimeline kasvama söötmesse lisatud suhkrul. Maltoosi indikaatorsööde võimaldab vahet teha tüvedel, mis fermenteerivad või ei fermenteeri maltoosi. Selleks kasutatakse rikkas söötmes süsiniku allikana maltoosi ning lisatakse värvainet bromo-kresool-punakaslilla (bromocresol purple). Maltoosi fermenteerivad rakud muudavad söötme pH-d ning koloonia ümber tekib kollane rõngas. Sööde ise aga on ...

Phospholipid-binding protein EhC2A mediates calcium-dependent translocation of transcription factor URE3-BP to the plasma membrane of Entamoeba histolytica ...

ANXA4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids (1-321 a.a.) and having a molecular mass of 36 kDa.

The worlds first wiki where authorship really matters. Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts.

Rabbit polyclonal Annexin-2/ANXA2 (phospho S26) antibody. Validated in WB and tested in Human. Immunogen corresponding to synthetic peptide.

To protect your privacy, your account will be locked after 6 failed attempts. After that, you will need to contact Customer Service to unlock your account.. You have 4 remaining attempts.. You have 3 remaining attempts.. You have 2 remaining attempts.. You have 1 remaining attempt.. Contact Customer Service ...

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Annexin A2 is a calcium regulated protein involved in membrane binding and actin polymerization. In this paper, Kevin Moreau and colleagues identified Annexin A2 as a…. ...

It has been reported that Annexin A2 (ANXA2) is up-regulated in hepatocellular carcinoma (HCC), but the roles of ANXA2 in the migration and invasion of HCC ...