TY - JOUR. T1 - Neonatal injection of Lewis rats with recombinant Vβ8.2 induces T cell but not B cell tolerance and increased severity of experimental autoimmune encephalomyelitis. AU - Vainiene, M.. AU - Burrows, G. G.. AU - Ariail, K.. AU - Robey, I.. AU - Vandenbark, A. A.. AU - Offner, H.. PY - 1996/1/1. Y1 - 1996/1/1. N2 - In Lewis rats with experimental autoimmune encephalomyelitis (EAE) mediated by Vβ8.2 effector cells, anti-idiotypic T cells and antibodies could be boosted by injection of Vβ8.2 peptides, inducing both T cells and antibodies that reduced the severity and shortened the course of disease. However, EAE in Lewis rats is self-limiting, and we sought to determine if the anti-idiotypic response contributed to the natural recovery process. In a previous study, we found that adult tolerance induced to one of the regulatory idiotopes, Vβ8.2-44-54, caused worsening of EAE, implicating response to this epitope in recovery from EAE. However, neonatally-induced tolerance to ...
The mammalian fetus affixed to the uterine wall in some ways resembles a homograft, but maternal homograft immunity even when specifically directed against her fetuses fails to destroy them. The placental barrier appears to be critical in protecting the fetus against maternal immunologic attack. In order to evaluate the means by which it affords protection, we have measured in rabbits the transfer of cytotoxic antibody from mother to fetus. When the offspring were not the specific targets for maternal antibody, we found titers of cytotoxic antibody in the newborn animals at or near maternal levels in 16 of 18 cases (89 per cent), demonstrating the ability of this antibody to cross the rabbit placenta. When the offspring were appropriate targets for antibody, however, 11 of 18 newborn animals (61 per cent) had no demonstrable titer. We believe that in these latter cases, antibody had become fixed to antigenic sites and thereby had been removed from the fetal circulation. There was, however, no ...
Besides the gross-out factor, however, there are other issues with this. First off, there is always a little concern that the dog will not take kindly to the boys matter-of-factly taking a toy out of his mouth. So far this hasnt been a problem, but it is always a worry. Secondly, the dog toys often have buttons and pieces that can come off and will not be safe for babies. Thirdly, the baby toys do not hold up to dog chewing, meaning our lovely set wood blocks that we searched for no less than 45 minutes at our local Target to find is now short about five blocks ...
Existing neonatal treatment intensity models can predict mortality and morbidity. Discriminatory performance as measured by the area under the receiver operating curve is poorest for long-term morbidity (0.59) and highest for in-hospital mortality in infants weighing 1000-1499 g for NTISS measured at 72 h post admission (0.958) [34, 39]. Calibration was reported by Gray et al. only who found a close agreement between observed and predicted in-hospital mortality by the Hosmer-Lemeshow test [19]. Using a variety of tests of statistical association rather than predictive performance, treatment intensity was found also to be associated with mortality, morbidity and resource utilisation [19, 35, 37, 39, 40].. Discriminatory performance of neonatal treatment intensity models for predicting in-hospital mortality measured by AUROC ranges from 0.749 to 0.958 [38, 39]. The recommended threshold for good discrimination is 0.8 [11]. The reported performance therefore suggests that the treatment scoring ...
In order to study the various trends and patterns prevailing in the concerned market, Fact.MR has included a new report titled "Fetal and Neonatal Heart Monitor Market" to its wide online database including upcoming trends and growth factors through 2018-2028. This research assessment offers a clear insight about the influential factors that are expected to transform the global market in the near future. The report studies the "Fetal and Neonatal Heart Monitor Market" worldwide, especially in North America, Europe, Southeast Asia, India and Other Regions with production, size, growth, revenue, consumption, import and export in these regions. The intelligent report also anticipates that the market would grow at a constructive CAGR until 2028.. Request for Sample of this Global Fetal and Neonatal Heart Monitor Market Research Report Here - https://www.factmr.com/connectus/sample?flag=S&rep_id=750. Preterm birth is one of the most prevalent cause of stillbirths, or fetal mortality, and neonatal ...
In this Application, we describe a protocol to generate highly purified and viable astrocytes from neonatal mouse brain tissue. - France
Pressure loads were applied to the right ventricles of newborn lambs and adult sheep by surgical narrowing of the lumen of the main pulmonary artery. Significant increases in right ventricular weight and in the diameter of the right coronary artery occurred in both age groups of animals, compared with corresponding control animals. The evidence suggests that the degree of increase in the arterial diameters, relative to the increase in ventricular muscle mass, is similar in both newborn and adult animals. However, the results suggest also that the capacity to respond to pressure loads, in terms of increased muscle mass, is relatively greater in the newborn animals than in the adults.. ...
The isolated perfused liver technique is the in vitro system most nearly comparable to the intact liver for experimental investigations on drug metabolism. The model currently used employs liver from different species, but only adults. For the first time, we have set up an experimental investigation involving perfusion of the liver of newborn animals. Using theophylline (TH) as tool drug, an in vivo/in vitro and adult/newborn disposition study was made in the rabbit. After a 10 mg/kg dose iv to adult rabbits and ip to rabbits at birth, the pharmacokinetic profile of TH was analyzed during liver perfusion at comparable TH concentrations in the medium. A few biochemical variables were recorded. No age-related differences were observed in the release of glutamic-oxalacetic transaminase and lactate dehydrogenase over the perfusion time. O2 consumption was higher in adults than in newborns, in accordance with the lower metabolic capacity of the neonatal liver, supported by the lower values of ...
Large populations (up to 600/cell) of spherical, electron-opaque granules ∼0.3 to 0.4 µ in diameter are characteristically found in muscle fibers of mammalian atria. They are absent in muscle fibers of the ventricles. The granules are concentrated in the sarcoplasmic core and occur in lesser numbers in the sarcoplasmic layers between myofibrils and under the plasma membrane. Their intimate association with a central voluminous Golgi complex and the frequent occurrence of material reminiscent of the granular content within the cisternae of the Golgi complex suggest that the latter is involved in the formation of the atrial granules. Atrial granules are larger and more numerous in smaller species (rat, mouse), and generally smaller and less numerous in larger mammals (dog, cat, human); they are absent from the atrial fibers of very young fetuses (rat) but are present in those of newborn animals. A small population of bodies containing glycogen particles and remnants of the endoplasmic reticulum ...
Functional testing showed that electrical stimulation of muscle fibers caused limbs to contract with 80% strength of what would be seen in newborn animals.
Great posts. Thanks. I am so glad to find this site for you have an appreciation for animals like I do. We were planning to buy 2 foxes from different sellers so we would be a propagator. It is the only way to keep some. Newborn animals is nothing new to us. We are used to around the clock care for new born animals. From dogs cats, llamas, horses, bot bellied pigs etc, etc. I fully understand the amount of work it will take to work with a fox. Although I do admit I lack the experience I do not lack the passion. We have to start some time and this is what the forum is for those who can not have more experience hands on. We do live in country so no ordinances or neighbors. The location of where I though of putting them is in a separate building which is on a slab of concrete. Has full plumbing and even a shower in there. After reading posts I plan of converting a window to an outdoor pen as well so they can have the best of both worlds. In the room I wasnplannin on filling it with dirt, woodchips, ...
Experience a busy spring day on a working 19th century farm! Help with spring planting in the large kitchen garden, sow oats in the fields or wash clothes in a washtub. Authentic demonstrations include blacksmithing, plowing with the horses and visiting newborn animals. Craft activities, shuttle rides to and from the Nature Center, refreshments and games make this a fun event.. This event is for all ages. Cost is $4 per person or $16 per family. Children ages 3 and younger are free. ...
Super 7+ Navel Dip is an umbilical cord dry-out and protective solution for newborn animals. This is a safe & effective alternative to strong iodine. The Navel Dip is NOT intended for use on wounds.
Find a Ole Miss Rebels NCAA Newborn Heart Short Sleeve Creeper at lids.com today! With our huge selection of Ole Miss Rebels gear, youll find what you need.
TY - JOUR. T1 - Abnormal maturation of cerebral cortex and behavioral deficit in adult rats after neonatal administration of antibodies to ganglioside.. AU - Kasarskis, Edward J.. AU - Karpiak, Stephen E.. AU - Rapport, Maurice M.. AU - Yu, Robert K.. AU - Bass, Norman H.. PY - 1981/1/1. Y1 - 1981/1/1. N2 - Five-day-old rats received a single injection (50 microliter) of antiserum to ganglioside into the cisterna magna and were compared to control animals injected with the antiserum which had been absorbed with pure GM1 ganglioside to remove the specific antibodies. Both groups showed normal rates of body growth. However, animals receiving antiganglioside serum had, at 60 days of age, impaired performance when tested on a complex learning task (DRL) as well as chemical and morphological alterations in the somatosensory cerebral cortex. Gross morphology and wet weight of whole brain were normal in both groups. Microchemical analysis of somatosensory isocortex revealed a normal content of total ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Plates 34A, 34B Lower Thoracic Section 1741 Total area: 1.6474 mm2Plates 35A, 35B Upper Lumbar Section 1821 Total area: 2.7717 mm2Plates 36A,
Video articles in JoVE about cranial nerves include Ocular Kinematics Measured by In Vitro Stimulation of the Cranial Nerves in the Turtle, Preparation of Rhythmically-active In Vitro Neonatal Rodent Brainstem-spinal Cord and Thin Slice, Intra-Operative Neural Monitoring of Thyroid Surgery in a Porcine Model, Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices, Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology, Slice Preparation, Organotypic Tissue Culturing and Luciferase Recording of Clock Gene Activity in the Suprachiasmatic Nucleus, Biomarkers in an Animal Model for Revealing Neural, Hematologic, and Behavioral Correlates of PTSD, Primary Orthotopic Glioma Xenografts Recapitulate Infiltrative Growth and Isocitrate Dehydrogenase I Mutation, Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology, Intranasal Delivery of
Colony stimulating factor-1 (CSF-1) regulates the survival, proliferation, and differentiation of mononuclear phagocytes. To determine whether CSF-1 plays a role in the perinatal development of these cells, CSF-1 protein and mRNA expression in tissues and serum from fetal/neonatal mice and their mothers was analyzed. As fetal/neonatal age increased, CSF-1 concentrations ...
From the evolutionary point of view it is quite interesting that often males and females are born in approximately similar numbers despite the fact stated by Rap, that you only need a few males to fertilize dozens of females, or even more. In many species this obviously has a lot to do with competition: the nature makes males to fight for their right to have offspring, thus ensuring that the best genes survive. But if so, why not make 2/3 males and 1/3 of females to make the competition even more fierce? Or make 1/3 males and 2/3 females simply to allow for more babies to be born. Also, human males are born at a slightly higher rate than females, is this because more males get themselves killed before they reach adult age ...
Oaktown Pup gonna give it to ya! After dancing to the latest Fetch! Pet Care Video Of the Week, youll know exactly what these talented pups are serving!
When you are feeling sore after giving birth, it is hard to even contemplate a poo. 10 expert tips to manage to your postnatal poos without pain.
This girl is in a high kill gas shelter - she gave birth to 9 pups soon after being brought in. Nothing like leaving her and the pups on a concrete floor - a blanket or a towel at least would be nice. Shes obviously scared to death. Please, please cross-post - this poor mom needs help fast for her and her babies ...
Sav-A-Caf® Ultra Start® Multi is a versatile multi-species colostrum supplement that is nutritionally complete. Provides 30 g protein from 100% pure Bovine colostrum.
TY - JOUR. T1 - Cell formation in the cortical layers of the developing human cerebellum. AU - Ábrahám, H.. AU - Tornóczky, T.. AU - Kosztolányi, G.. AU - Séress, L.. PY - 2001/2/1. Y1 - 2001/2/1. N2 - Cell proliferation has been studied in the human cerebellar cortex between the 24th gestational week and the 12th postnatal month. Intensive cell formation has been found in the external granular layer (EGL) of the human cerebellum, where the highest cell proliferation rate occurs between the 28th and 34th gestational weeks. This is followed by a gradual decrease that lasts up to the eighth postnatal month. As late in development as the fifth postnatal month, still 30% of cells of the EGL are labeled with the monoclonal antibody Ki-67, which is specific for dividing cells. The width of the EGL remained unchanged from the 28th gestational week to the end of the first postnatal month, when it starts to decrease and completely disappears by the 11th postnatal month. Large number of Ki-67 labeled ...
The purpose of this study was to gain more information on the structure of different layers of renal arteryand comparing these structures in post-natal male and female sheep. To do so, right and left renal arteries of6 adult and 6 newborn animals were dissected; the middle parts of arteries were only collected. After tissueprocessing using paraffin embedding method, 5-6-μm sections were cut and stained with haematoxylin andeosin, green Massons trichrome and Verhoeffs elastic fiber methods. Three layers were identified in thewall of artery in both sexes. In tunica media the number of rows of circular smooth muscle cells was 15-25rows in newborn and 30-40 in adult sheep. External elastic membrane was visible in adult and wasstructurally thinner than the internal membrane. Tunica adventitia was visible in all samples and collagenfibers and smooth muscles bundle were distinguished. The existence of these muscle bundles in externallayer of renal artery was not reported previously in domestic animals and
Fact.MR has adopted multi-disciplinary approach to shed light on the evolution of the global Fetal and Neonatal Heart Monitor market during the historical period of 2013 - 2017. The study presents a deep-dive assessment of the current growth dynamics, major avenues in the estimation year of 2018, and key prospects over the forecast period 2018 - 2028.. Extensive rounds of primary and a comprehensive secondary research have been leveraged by the analysts at Fact.MR to arrive at various estimations and projections of the FETAL AND NEONATAL HEART MONITOR market, both at global and regional levels. The analysts have used numerous industry-wide prominent business intelligence tools to consolidate facts, figures, and market data into revenue estimations and projections in the FETAL AND NEONATAL HEART MONITOR market.. Global sales of fetal and neonatal heart monitors are likely to surpass 261,000 units in 2018, with rising adoption in the US driving sales. Cardiotocographs remain the highest selling ...
Ian Tizzard (From Proceedings Ruvasa Congress 2016). When a mammal is born, it emerges from the sterile uterus into an environment where it is immediately exposed to a host of microorganisms. Its surfaces acquire a complex microbial flora within hours. If it is to survive, the newborn animal must be able to control this microbial invasion. In practice, the adaptive immune system takes some time to become fully functional, and innate mechanisms are responsible for the initial resistance to infection. In the domestic mammals, the adaptive immune system is fully developed at birth but cannot function at adult levels for several weeks. The complete development of adaptive immunity depends on antigenic stimulation. Thus, newborn mammals are vulnerable to infection for the first few weeks of life. They need assistance in defending themselves at this time. This temporary help is provided by the mother in the form of antibodies from colostrum and milk. The passive transfer of immunity from mother to ...
Due to the special growth and developmental characteristics of newborn infants, VILI is thought to play an important role in the pathogenesis of neonatal chronic lung disease, which has an adverse effect on the survival rate and the quality of life for newborns with ALI/ARDS. To study newborn ALI/ARDS, newborn animals, such as piglets,5,6 rats,7 and rabbits,8 have been used as animal models. Because the organ development, lung volume, and birth weight of newborn piglets resemble those of newborn infants, the current study used induced ALI/ARDS in newborn piglets.. One of the aims in this study was to compare the histopathological features of the lung damage in gravitation-dependent and gravitation-nondependent regions. Compared to the gravitation-nondependent region, the gravitation-dependent region displayed increased lung swelling and hemorrhaging, and damage to the alveolar integrity and organization. We observed increased inflammatory cell infiltration, edema formation, and hemorrhages, ...
A light-and electron-microscopic study of pig hepatocytes from late prenatal to early neonatal animals shows changes which reflect an increasing rate of synthetic activity. The granular endoplasmic reticulum (ER) in the prenatal pig hepatocyte is situated along the periphery of the cytoplasm and in the region immediately surrounding the nucleus. Mitochondria are most abundant in the area adjacent to the nucleus, while the Golgi complex is generally located in the region of the bile canaliculus. The remaining portion of the hepatocyte is occupied with glycogen. A few hours after birth the hepatocyte increases about twofold in size with the nucleus shifting from a peripheral to a more centrally located position. The glycogen decreases quickly coincident with a rapid increase in the amount of granular ER and the dispersion of the mitochondria throughout the cell. The Golgi complex becomes distended and numerous vesicles appear in its immediate vicinity containing a moderately dense material. ...
Durvet Soluble Colostrum Powder designed to assist in the replacement of colostrum in deficient and newborn calves, sheep and goats. Dried colostrum provides a source of maternal antibodies and protein; lactobacillus organisms assist in the establishment of beneficial microflora in newborn animals.
TY - JOUR. T1 - Copper in fetal and neonatal development.. AU - Hurley, L. S.. AU - Keen, Carl L. AU - Lönnerdal, B.. PY - 1980. Y1 - 1980. N2 - The essentially of copper for normal fetal and neonatal development has been well documented, although copper metabolism during this period is poorly understood. The dietary requirement for copper is influenced by genetic background. The neurological phenotypic characteristics of the mutant gene quaking (qk) in mice resemble in part those of copper-deficient animals. Supplementation of the maternal diet with copper during pregnancy and lactation, or during lactation alone, greatly reduced the frequency of tremors characteristic of these mutants, and brought the otherwise low copper concentrations in the brain to normal. Prenatal copper supplementation of crinkled (cr) mice increased neonatal survival and produced nearly normal development of skin and hair. Non-supplemented cr/cr mice showed anaemia at 21 days of age which disappeared later. Similarly, ...
It has been speculated whether the metabolism of the pre-implantation embryo may be reflected on the pregnancy and characteristics of the newborn animal. The present study investigated whether respiration rates of individual embryos were correlated with gestation length, type of parturition, birth weight, sex, and viability of the offspring. In-vivo embryos (n = 47) were recovered on day 7 from 11 superovulated and inseminated Holstein-Friesian cows and embryonic respiration rates were measured individually by nanorespirometry. The embryos were classified according to morphological quality (I, II, III), stage of development, and diameter and were subsequently transferred individually (n = 43) to synchronized recipients. Gestation length of the recipients (n = 22) was calculated and the type of parturition (no assistance, light traction, heavy traction, or caesarean section) recorded. Sex, weight, and condition of the calves at birth (weak, normal, or very active) were also assessed. Results were ...
Transport systems for organic compounds are immature in the kidney of most newborn animals. Measured in vitro, uptake of p-aminohippuric acid (PAH) into renal cortical slices is low in tissue from 2-week-old rabbits, peaks at 4 weeks and declines to adult levels. To minimize limitations encountered with the slice technique, a separated tubule preparation was employed to estimate transport. Segments of proximal tubules were prepared by incubating renal cortex in 0.375% collagenase-Ringer solution. The tubules were washed, filtered, suspended in a Ringer-acetate solution containing dilute PAH, oxygenated and incubated at 25°C. The tubules from young animals consumed oxygen to the same extent as those from adult. Intracellular concentrations of sodium and potassium were similar at each age tested. Maximal PAH uptake (T/M ratio) was Obtained after approximately 30 minutes of incubation in tissue from animals of all ages. Adult T/M ratios were significantly higher in the presence of acetate. The ...
Cells and virus preparation. Transformed fetal liver cells, bone marrow cells, and tumor-derived cell lines were maintained in RPMI medium containing 10% FCS, 100 U/ml penicillin/streptomycin, and 5 μM β-mercaptoethanol. A010 cells that produce an ecotropic replication-deficient form of the Abelson virus were maintained in DMEM containing 10% FCS and 100 U/ml penicillin/streptomycin. Viral supernatant was prepared as previously described (49).. Mice and infection of neonatal mice with A-MuLV. TYK2-/- mice were described previously (25) and were backcrossed to C57BL/6 for 8 generations. Nude mice were purchased from the Institut für Labortierkunde und Genetik (Himberg, Austria).. For the infection of newborn animals, TYK2+/- were bred with TYK2-/- animals. Hence, every litter contained TYK2+/- and TYK2-/- animals to ensure appropriate controls. We initially started the in vivo transformation experiments by intercrossing TYK2+/- and TYK2+/- mice and analyzed leukemia/lymphoma formation in all 3 ...
Cerebral hypoxia-ischemia in human infants presents a complex clinical problem in that no standardized treatment currently exists. With an understanding of the cellular and metabolic changes brought about during hypoxia-ischemia in the immature brain, researchers can better understand the course of damage caused by ischemia. Such damage was induced in young rats in an attempt to learn more about the mechanisms of hypoxia-ischemia. Seven-day-old rat pups underwent permanent unilateral carotid artery ligation and then were exposed to systemic hypoxia. At 15 days of postnatal age, researchers used neuropathologic analysis, gross examination, and staining of brain slices to assess the severity of damage from hypoxia-ischemia. Hypoxic preconditioning appeared to reduce or prevent tissue damage during a subsequent hypoxic-ischemic event.
rotavirus: Any of a group of wheel-shaped, RNA-contained viruses that cause gastroenteritis, especially in infants and newborn animals.
The story of a gorilla, from birth to adulthood, photographed on location in the wild by an award-winning American photographer, who specialises in work with newborn animals. The text will show all the aspects of the animals life in the wild, accompanied by close-up pictures of the family group in its natural habitat. A spread at the back of the book will give further conservation information, including useful websites ...
Controlled Iodine Spray is indicated for use in cattle, horses, swine and sheep as a topical antiseptic for use prior to surgical procedures such as castrating and docking; for ringworm and foot rot; for application to the navel of newborn animals; and for minor cuts, teat sores, bruises and abrasions ...
We have investigated the dynamics of meiotic prophase I in neonatal ovaries from different wild rodent species, from a laboratory strain ofMus musculus and
For some time it has been thought that antigenic challenge in neonatal life is a tolerogenic rather than immunogenic event. Reexamination of the classic neonatal tolerance experiments of Billingham, Brent, and Medawar showed that tolerance is not an intrinsic property of the newborn immune system, but that the nature of the antigen-presenting cell determines whether the outcome is neonatal tolerance or immunization.
Animals and reagents. Newborn Sprague-Dawley rats (18-20 g) were supplied by the Animal Center of Guangzhou Medical University. Rats were housed and used in accordance with our institutional guidelines for animal care and the Guide for Animal Care of the National Institutes of Health. Dulbeccos modified Eagles medium (DMEM), fetal bovine serum (FBS), and TRIzol were purchased from Gibco BRL (USA). Trypsin was obtained from Amersco (USA). Alpha-sarcomeric actin was purchased from Santa Cruz Biotechnology (USA). The SYBR®GreenPCR Master Mix was obtained from Applied Biosystems (USA). The Thermo ScriptRT-PCR kit was purchased from Invitrogen (USA). [3H]-leucine (Leu) was obtained from the China Institute of Atomic Energy (China) and recombinant Ang II and HGF were purchased from Sigma (Germany).. Primary cardiomyocyte culture. Rat neonatal ventricular cardiomyocytes were prepared as previously described, with some modifications (17). The cells were suspended in DMEM containing 10% FBS and 0.1 mM ...
One strategy we are undertaking to advance this work is to characterize the antigen-presenting environment in pre- and post-natal life, with the focus on defining factors that dictate whether exposure to allergen in early life induces T cell sensitization or tolerance. The thinking is that in certain circumstances, T cell sensitization can occur in utero or in early post-natal life and that factors responsible for these circumstances have increased in the past 20 years. It has been proposed that this could explain the increased incidence of asthma and other inflammatory diseases. The challenge for scientists is to identify factors that control this process, with the ultimate goal of developing therapeutic strategies to reverse the progressive increase in disease. Our studies are focused on antigen-presenting cells such as dendritic cells (DCs) or macrophages, as they are known to direct the T cell responses to antigen throughout life, presumably including the pre- and post-natal period. In ...
Semantic Scholar extracted view of Effects of prenatal exposure to 2,4-D/2,4,5-T mixture on postnatal changes in rat brain glutamate, GABA, protein, and nucleic acid levels. by Fouad Kasim Mohammad et al.
Fingerprint Dive into the research topics of Angiotensin II type 1 receptor antagonist attenuates lung fibrosis in hyperoxia-exposed newborn rats. Together they form a unique fingerprint. ...
Journal Article: Neural Stem Cells Expressing bFGF Reduce Brain Damage and Restore Sensorimotor Function after Neonatal Hypoxia-Ischemia. Ye, Qingsong, Wu, Yanqing, Wu, Jiamin, Zou, Shuang, Al-Zaazaai, Ali Ahmed, Zhang, Hongyu, Shi, Hongxue, Xie, Ling, Liu, Yanlong, Xu, Ke, He, Huacheng, Zhang, Fabiao, Ji, Yiming, He, Yan and Xiao, Jian (2017) Neural Stem Cells Expressing bFGF Reduce Brain Damage and Restore Sensorimotor Function after Neonatal Hypoxia-Ischemia. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 45 1: 108-118. doi:10.1159/000486226. ...
The level of Fc receptor rosette-forming lymphocytes (Fc-RFL) was examined in spleen and lymph node cell suspension from neonatal DA and BN rats inoculated within 24 hr of birth with either allogeneic L (experimental) or syngeneic (control) lymphoid cells. In addition, these levels were compared to fetal and neonatal animals that received no injection. The indicator cells (EA) were sheep erythrocytes sensitized with one-half concentration of the highest dilution of rabbit anti-sheep erythrocyte IgG(A) which agglutinated an equal amount of 1% suspension of E. Care was taken to exclude scoring macrophages by injecting colloidal carbon at least 1 hr before killing the test animals. The spleen of 19-day DA fetal rats exhibited a level of 19.3% Fc-RFL, similar to that of animals having received adult syngeneic cells at birth (40.0%) by day 7. Thereafter the level of Fc-RFL did not vary appreciably between these two groups. However, as early as 2 days after inoculation there was a significantly greater
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Although some invertebrates can regrow parts of their hearts after damage, that has not been known to be possible for mammals. Until now, that is. Hesham Sadek and his colleagues from the University of Texas Southwestern Medical Center have observed heart regeneration in newborn mice.. The researchers removed 15% of the heart tissue from newborn mice. To their surprise, the infant mice were able to regrow the tissue. The resultant hearts looked and performed exactly like normal hearts. This ability was retained until the mice were seven days old, at which point they lost the capacity to grow new heart muscle. To put that in perspective, mice reach maturity between 6 to 8 weeks and can live for two or three years. Thus, the ability to fix heart problems continues for a large part of the mouses childhood.. The scientists believe that the infant mices cardiomyocytes, the beating cells of the heart, can stop beating and divide, repairing the damage. The next step is to figure out whether adult ...
Chronic kidney disease affects more than 10% of the population. Programming studies have examined the interrelationship between environmental factors and differences in morbidity and mortality between individuals. A number of important principles has been identified, namely permanent structural modifications of organs and cells, long lasting adjustments of endocrine regulatory circuits, as well as altered gene transcription. Risk factors include intrauterine deficiencies by disturbed placental function or maternal malnutrition, prematurity, intrauterine and postnatal stress, intrauterine and postnatal overnutrition, as well as dietary dysbalances in postnatal life. This mini-review discusses critical developmental periods and long term sequelae of renal programming in humans and presents studies examining the underlying mechanisms as well as interventional approaches to
Maturational differences in lung NF-κB activation and their role in tolerance to hyperoxia: Neonatal rodents are more tolerant to hyperoxia than adults. We dete
Caring for newborn kittens is a great responsibility that can be quite easy, enjoyable and rewarding if you know what to do. This guide will help.
The neonatal murine retina provides a well characterized physiological model of angiogenesis, which permits investigations of the roles ...
Effective neonatal immunization of human beings has proven challenging. results CHIR-265 may possess essential implications for immunization of human being babies. Newborns are at risk for exposure to many infectious diseases, yet vaccination generally is not carried out until 2C3 months of age, owing to the immaturity of the neonatal immune system (1). In particular, B cell responses are weak and preferentially generate IgM/IgG1 antibody isotypes, and cytotoxic T lymphocyte (CTL) responses are CHIR-265 poor (see ref. 2). In addition, maternally derived antibodies can interfere with the vaccine (3C6). Young mice are useful models to test immunization strategies for newborn humans since their response to protein antigens has similar limitations (7). Although it has been thought that immunization early in life would induce immunological tolerance (8C11), humoral responses have been induced in newborn mice against a variety of antigens (12C14). This recently has been shown to depend on an ...
I was first surprised by how friendly, welcoming, and open the LoTurco lab was. The graduate students and other undergrads were very open to sharing and explaining their research. One graduate student scheduled time for me to observe an analysis using the microscopy labs TEM (Transmission Electron Microscope), and another let me observe her calcium imaging and stimulation in the brains of neonatal mice. I was also surprised by how willing they were to help me when I was unsure of exactly how to use a certain device, and how eager they were to discuss the topics of papers I had questions about.. Im fascinated by all aspects of the brain - especially its development, functioning, and regulation - so all the projects the lab is working on interested me. I was particularly interested in one students work with calcium imaging in the development of neonatal mice brains. The study involves the dissection and subsequent stimulation of mice brains, analyzing calcium signaling in response to various ...
Animals. Ntn1+/-, Cx40-GFP, flk1-GFP, mTmG, Sm22-Cre, TH-Cre, and Dccfl/fl mice were described previously (21-23, 29, 30, 56, 57). L. Miquerol (UMR 7288, Marseille, France) provided Cx40-GFP mice. Ntn1fl/fl mice were generated by Ozgene. LoxP sites were inserted on both sides of exon 3 of the mouse Ntn1 gene, which contained the initiation codons for netrin-1 full-length and short nucleolar forms (58). Cre recombination and exon 3 deletion are predicted to produce a misfolded protein truncated of its 353 N-terminal amino acids, lacking the signal peptide and first 2 laminin EGF-like domains.. Neonatal mouse injections. At day P1, P2 wild-type pups on a CD1 background (n = 6 per group) received intraperitoneal injections of 20 μg/g anti-netrin-1 antibody, 2.5 μg/g anti-DCC antibody (Calbiochem), or vehicle PBS. Neonatal pups were collected for analysis at day P3. The Dccfl/fl TH-CreER mice were injected with 100 μg 4-OHT at P0, and tissues were collected at P3. For gain-of-function ...
At birth, some infants are already saddled with brains that carry features of Alzheimers disease and schizophrenia. Newborns who carry certain versions of genes already show brain shrinkage reminiscent of that in adults with brain illnesses, a study of 272 newborn babies reveals.. ...
Central vagal neurons receive both glycinergic and GABAergic inhibitory inputs at early postnatal timepoints, but adult vagal efferent motoneurons receive only inhibitory GABAergic synaptic inputs. This surely points to the loss of glycinergic inhibit
... : Cardiovascular transcription factor expression in isolated neonatal sheep cell clones. Neonatal sheep cardiovascular cell clones shown were tested by PCR for expression of Isl 1 and GATA-4. All seven clones expressed transcripts for Isl1 (A, Isl1 size=131bp) and a proportion of these clones expressed transcripts for GATA-4 (B, GATA-4 size=188bp ...
This cow wants to rock n roll all night and party every day -- or so wed assume, if shes anything like her human doppelgänger .
Olive oil plays a key role in foetal development during pregnancy and a shortage may have pernicious effects on the baby"s subsequent development. It has been demonstrated that the post-natal development of babies of mothers who consumed olive oil when pregnant is better in terms of height, weight, behaviour and psychomotor reflexes. The foetus needs ...
Most marine fish larvae tend to live in surface or near-surface waters, while adult fish inhabit largely different environments. The different habitats require two different body shapes, leading to larvae that look very different from their adult counterparts.. ...
Most marine fish larvae tend to live in surface or near-surface waters, while adult fish inhabit largely different environments. The different habitats require two different body shapes, leading to larvae that look very different from their adult counterparts.. ...
Male mice are not natural-born fathers. Males that have never mated respond with aggression to chemical signals from newborn mice pups, whereas those that have fathered pups are more nurturing, a new study finds.
Puppies love and bond to one another in a way even more than they bond to their mother or us. The siblings form their first "pack", although a true pack of canines is quite a different structure since it involves non-kin and overtones of sexuality that these little guys lack. So the Puppy Pack is more of a play group--and in some ways, it is the nostalgic youth that we all seek: no responsibilities, plenty of warmth and novelty, strong ties to your fellow creatures, sleeping piles, an abundance of food, virtually no imposed rules (!), etc. Whats not to like about this ...
Subjects: Wistar rats. Chemical: Dioxin (TCDD). Low doses tested: 1.0 μg/kg bw. Route of administration: dissolved in corn oil and administered orally. Exposure duration: gestational day 15 - single dose (comparable to human prenatal development from approximately day 1 of week 6 to day 1 of week 7). Age of measurement: gestational day 20, post-natal days 21, 49, 56, 70, 91, 98, and 10 and 13 weeks of age ...
The profitability of a sheep enterprise depends on the number of lambs sold either for meat or as breeding stock. The number raised to market is a reflection of the complete management of the flock throughout the year. One of the critical points in this management cycle is lambing.
Your precious newborn will stand out with the cutest hat in the Nursery! This Pink and White Newborn Hospital Beanie is perfect for your newborn. It has a matching Pink and White Bow for the perfect touch! (Light Pink ribbon shown) Great for her first hospital pictures or to keep her
It can be disconcerting to see what may look like an infection on your babys skin. Newborns are prone to a variety of rashes, but staph itself does not...
Because some potential problems arent readily seen at birth, all newborns are tested for certain conditions, including metabolic disorders.
I have 2 pups (1male 1 female) left from a litter whelped in April. Sire is NC Shadows Attitude Dam is Daughter of HonkyTonk Highrise. Both pups are mar
Kinetic analysis of virus titers in newborn inoculated CBA/J mice. Spleens from neonatally infected mice were harvested, homogenized, and titered for polyoma vi
One of the least discussed topics in pig nutrition is how to feed the sow after piglets are weaned and until she shows the first signs of estrous. There are many programs, ideas, and methods! They range from starvation in the first couple days to feeding ad libitum to achieve the so-called 'flushing effect'. Based on my experience, no two farms use the same method, and there is at least more than a few programs that are used successfully.
(a) Developmental changes in the proportions of GABAergic and glycinergic synaptic activity in various areas of the central nervous system. (b) Examples of ind
A newborns growth and development is measured from the moment of birth. Find out if your babys size is normal, and what to expect as your baby grows.
Regardless of where they are born in the United States, nearly all newborns now receive mandated screening for many life-threatening disorders.
Your newborn sleeping on side or rolling to their tummy? Many new moms asked me this question and Im trying to give you a clear answer on this post.
As medical records become electronic, one important category was left out: Newborn screening, meaning treatment for congenital conditions has been delayed.
Any ideas on best way to describe what jitteriness is in a newborn??? Thats such a vague term.... Thanks for any help you can offer me!!
Play is the primary way that infants learn how to move, communicate, socialize, and understand their surroundings. And during the first month of life, your baby will learn by interacting with you.
Long periods can be a nuisance, or it can indicate a serious problem. Learn about the causes and treatment of excessive menstrual bleeding.
We get a lot of questions at Scarleteen from folks who are worried about periods that are MIA (missing in action, for us civilians). Sometimes theres a pregnancy concern, and sometimes not; but even if youre not sexually active, a missing period can be worrying.
How to Ask About Getting Your Period. Getting your period can be a scary and uncertain time in your life. Some girls get ridiculed for starting their periods, but it is truly a natural process that happens to millions of girls and women...
Sometimes life can bring us to a point where we need to do our past healing work. Going back into our pasts can be painful, but this is where need to go in order to be set free.
A July 2005 study by the Environmental Working Group tested newborns for the largest number of chemicals ever. The analysis found an average of 200 industr
I am starting my second week on P-Plex running 20/20/30/30/30.. I have nolva for PCT but I want to know when, if any, is a proper time to take an anti-
Hypoxic-ischemic encephalopathy (HIE) is a leading cause of mortality and morbidity during the perinatal period, and currently no therapeutic drug is available. Minocycline, an antibiotic, has recently been shown to have neuroprotective effects distinct from its antimicrobial effect in several neurological disorders including ischemic brain injury. We examined the effect of minocycline on neonatal hypoxic-ischemic brain injury by using histologic scoring in both mouse and rat models. Mouse (C57Bl/6) and rat (SD) pups were exposed to a unilateral hypoxic-ischemic insult at 8 and 7 days of age, respectively. Minocycline hydrochloride was administered according to protocols that were reported to provide neuroprotection in adult or neonatal rats. Seven days after the insult, we examined brain injury in Nissl stained sections. Although minocycline ameliorated brain injury in the developing rat, it increased injury in the developing mouse. This detrimental effect in the mouse was consistent across ...
The new findings of the present study are: (1) perinatal nicotine exposure significantly increases brain vulnerability to HI injury in male rat pups, but not in female pups; (2) this heightened vulnerability is associated with sex-specific reprogramming of AT1R and AT2R expression patterns in the developing brain; (3) both AT1R and AT2R are implicated in the pathogenesis of HI brain injury and exhibit the neuroprotective effect in neonatal brains; (4) downregulation of AT2R in the developing brain plays a causal role in nicotine-induced, heightened brain vulnerability to HI injury in neonatal rats; and (5) increased methylation of CpG locus 3 bases upstream of TATA-box at the AT2R promoter is a mechanism of nicotine-mediated AT2R gene repression.. The present finding that perinatal nicotine exposure increased brain HI injury in neonates is novel and suggests a risk factor of maternal cigarette smoking in heightened brain HIE vulnerability in newborns. The nicotine dose used in the present study ...
NRMU - Newborn Rhesus Monkey Uterus. Looking for abbreviations of NRMU? It is Newborn Rhesus Monkey Uterus. Newborn Rhesus Monkey Uterus listed as NRMU
TY - JOUR. T1 - Factors Known to Influence the Development of Necrotizing Enterocolitis to Modify Expression and Activity of Intestinal Alkaline Phosphatase in a Newborn Neonatal Rat Model. AU - Rentea, Rebecca Maria. AU - Rentea, Matthea J.. AU - Biesterveld, Ben. AU - Liedel, Jennifer L.. AU - Gourlay, David M.. PY - 2018/5/3. Y1 - 2018/5/3. N2 - Introduction Prematurity, formula feeding, and early weaning strongly influence enterocyte differentiation. Intestinal alkaline phosphatase (IAP), an endogenous protein expressed in the intestines, is one enzyme that is affected by these factors. IAP supplementation decreases the severity of necrotizing enterocolitis (NEC) injury. We, therefore, hypothesized that prematurity predisposes this population to NEC due to IAP deficiency and investigated IAP expression and function in a neonatal rat model. Materials and Methods Pre- and full-term newborn Sprague-Dawley rat pups were sacrificed on consecutive days of life both after vaginal or cesarean birth ...
Neonatal hypoxic-ischemic insults are a significant cause of pediatric encephalopathy, developmental delays, and spastic cerebral palsy. Although the developing brains plasticity allows for remarkable self-repair, severe disruption of normal myelination and cortical development upon neonatal brain injury are likely to generate life-persisting sensory-motor and cognitive deficits in the growing child. Currently, no treatments are available that can address the long-term consequences. Thus, regenerative medicine appears as a promising avenue to help restore normal developmental processes in affected infants. Stem cell therapy has proven effective in promoting functional recovery in animal models of neonatal hypoxic-ischemic injury and therefore represents a hopeful therapy for this unmet medical condition. Neural stem cells derived from pluripotent stem cells or fetal tissues as well as umbilical cord blood and mesenchymal stem cells have all shown initial success in improving functional ...
The present study investigated the involvement of capsaicin-sensitive sensory neurons on salt intake control in the rat, following capsaicin neonatal treatment. Capsaicin did not affect salt appetite induced by intramuscular injection of deoxycorticosterone enantate, or by intracranial injection of renin. Moreover, it did not alter salt preference of rats given access to a variety of NaCl concentrations, or the need-free salt intake of multidepleted male rats. On the other hand, in response to furosemide-induced sodium depletion, the salt intake of capsaicin-treated rats was lower than that of controls. However, furosemide-induced Na+ excretion of capsaicin-treated rats proved to be lower than that of controls, thus suggesting that difference in salt intake might be secondary to lower sensitivity of capsaicin-treated rats to the natriuretic action of furosemide. Salt intake is known to be influenced by sensory information from the oral cavity, from the liver and from the intravascular ...
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The second messenger cyclic adenosine monophosphate (cAMP) is the most important modulator of sympathetic control over cardiac contractility. In cardiac myocytes and many other cell types, however, cAMP transduces the signal generated upon stimulation of various receptors and activates different cellular functions, raising the issue of how specificity can be achieved. In the general field of signal transduction, the view is emerging that specificity is guaranteed by tight localization of signaling events. Here, we show that in neonatal rat cardiac myocytes, beta-adrenergic stimulation generates multiple microdomains with increased concentration of cAMP in correspondence with the region of the transverse tubule/junctional sarcoplasmic reticulum membrane. The restricted pools of cAMP show a range of action as small as approximately 1 micrometer, and free diffusion of the second messenger is limited by the activity of phosphodiesterases. Furthermore, we demonstrate that such gradients of cAMP specifically
BACKGROUND: The occurrence of hypoxia-ischemia during the perinatal period leads to neuronal damage resulting in behavioral and psychological dysfunctions, cerebral palsy, epilepsy or even death. Despite the critical clinical and socio-economic consequences of perinatal brain damage, no effective clinical therapeutic strategy has yet to be developed. Herein, we sought to determine if hyperbaric oxygen (HBO) is able to offer neuroprotectivity against a hypoxia-ischemia insult. METHODS: Seven-day-old rat pups were subjected to unilateral carotid artery ligation followed by 2-2.5 hrs of hypoxia (8percent O2 at 37"aC). Pups were treated with 100percent oxygen one hour after the hypoxia-ischemia insult in an HBO chamber pressurized to 3 atmospheres absolute (ATA) for a duration of 1hr. Brain injury was assessed by brain weight (ipsilateral/contralateral) , light microscopy, and sensorimotor functional tests 2wks, 5wks, and 6wks after hypoxia-ischemia. Apoptotic cell death was examined by EM, TUNEL ...
Newborn Calf CR colostrum replacer for calves from Agrilabs provides essential globulin proteins, lipids and carbohydrates to give newborn calves a healthy start. Contains...
Background: The zinc finger transcription factor Egr-1 (Early growth response 1) is central to several growth factors and represents an important activator of target genes not only involved in physiological processes like embryogenesis and neonatal development, but also in a variety of pathophysiological processes, for example atherosclerosis or cancer. Current options to investigate its transcription and activation in vivo are end-point measurements that do not provide insights into dynamic changes in the living organism. Results: We developed a transgenic mouse (Egr-1-luc) in which the luciferase reporter gene is under the control of the murine Egr-1 promoter providing a versatile tool to study the time course of Egr-1 activation in vivo. In neonatal mice, bioluminescence imaging revealed a high Egr-1 promoter activity reaching basal levels three weeks after birth with activity at snout, ears and paws. Using a model of partial hepatectomy we could show that Egr-1 promoter activity and Egr-1 ...
The chemokine SDF-1α and its receptor, CXCR4, are known to play important roles in neural development (Tran and Miller, 2003). The present study unveiled several features of SDF-1α and CXCR4 during the early developmental stage of the hippocampus. First, the histochemical analysis revealed that SDF-1α is distributed in the DG neurons and that CXCR4 is expressed in the EC neurons of rats at postnatal day 0. Consistent with this observation, previous studies have reported that SDF-1α mRNAs are expressed in the granule cell layer of the DG in the brains of early postnatal rats (Tham et al., 2001; Berger et al., 2007). These data prompted us to hypothesize that SDF-1α expression in the DG induces axonal elongation of EC neurons expressing CXCR4.. SDF-1α promotes the neurite growth of dissociated EC neurons, and the neutralizing anti-SDF-1 antibody as well as AMD3100, a CXCR4 antagonist, abolishes this effect of SDF-1α. Because previous studies have demonstrated that SDF-1α promotes axonal ...