The most important thing to remember regarding field safety is that all the same requirements and regulations that must be followed in labs and animal facilities must also be adhered to in the field! This includes proper handling, storage, labeling, shipping, and disposal of all materials and appropriate use of PPE. Field work with animals has the same risks as work conducted in animal facilities, but those risks are compounded by risks associated with climate/weather, terrain, native plants, insects, wildlife and distance from help. Work with your unit safety officer and/or EHSRM to assess the risk factors associated with your specific location(s) and activities. The UA Statewide Office of Risk Management has published two documents related to remote travel: the UA Remote Travel Safety Guide and the UA Remote Travel Planning & Resource Guide. These documents provide excellent suggestions on how to prepare and pack for remote travel and address the most common health and safety risks encountered ...

c. Lyme Disease: Lyme disease (Borrelia burghdorferi) is a bacterium commonly infecting deer, white footed mice, and possibly other species that is transmitted to humans by ticks or occasionally by direct contact with the infected vertebrate. You must have a tick drinking your blood for at least 16 hours to contract this disease. Removal of a tick before that length of time is preventative. A reddish halo (bulls eye ring around the bite) sometimes forms approximately 2-12 weeks following a tick bite, and is the earliest sign of Lyme disease. After this, severe illness may develop including: heart inflammation with arrhythmia, arthritis, and nervous system disorders including peripheral nerve dysfunction.. d. Plague: Plague is cause by the bacterium, Yersinia pestis. The pathogen is transmitted by fleas but it also can be transmitted from human to human in its pneumonic form (a lung infection). Plague primarily is a disease of rodents. However, in Montana, the bacteria thrive in prairie dog ...

No person reporting an animal welfare concern in good-faith will be subject to retaliation according to the Animal Welfare Act and the University of Iowa Operations Manuals Anti-Retaliation Policy. The humane care and use of animals is of paramount importance to the University of Iowa. Individuals who have specific concerns about the treatment of animals, including animal husbandry concerns, should report their observations to the Chair or Director of the Institutional Animal Care and Use Committee (IACUC) for investigation.

The Acting Administrator of the Animal and Plant Health Inspection Service, United States Department of Agriculture [hereinafter Complainant], instituted this disciplinary administrative proceeding under the Animal Welfare Act, as amended (7 U.S.C. §§ 2131-2159) [hereinafter the Animal Welfare Act]; the regulations and standards issued under the Animal Welfare Act (9 C.F.R. §§ 1.1-3.142) [hereinafter the Regulations and Standards]; and the Rules of Practice Governing Formal Adjudicatory Proceedings Instituted by the Secretary Under Various Statutes (7 C.F.R. §§ 1.130-.151) [hereinafter the Rules of Practice], by filing a Complaint on February 22, 1996.. The Complaint alleges that: (1) on October 5, 1994, November 15, 1994, and January 31, 1995, Samuel Zimmerman [hereinafter Respondent] failed to maintain complete records showing the acquisition, disposition, and identification of animals in willful violation of section 10 of the Animal Welfare Act (7 U.S.C. § 2140) and section 2.75(a)(1) ...

The Southeastern Electric Exchange Joint Use Committee Meeting will be held April 18-19, 2017 in Atlanta, Georgia. KH Partner Tom Magee will participate in a legal panel. For more information, please contact Tom Magee ([email protected]; 202.434.4128).

Animal welfare in the United Kingdom relates to the treatment of animals in fields such as agriculture, hunting, medical testing and the domestic ownership of animals. It is distinct from animal conservation. The Animal Welfare Act 2006 is the latest animal welfare legislation in England and Wales. It superseded and consolidated more than 20 other pieces of legislation, such as the Protection of Animals Act 1934 and the Abandonment of Animals Act 1960. The 2006 Act introduced tougher penalties for neglect and cruelty, including fines of up to £20,000, a maximum jail term of 51 weeks and a lifetime ban on some owners keeping pets. Enforcers of the act such as the police or local authority inspectors (but not organisations such as the RSPCA) have more powers to intervene if they suspect a pet is being neglected. The Animal Welfare Act 2006 also introduced a welfare offence for the first time. This places a duty of care on pet owners to provide for their animals basic needs, such as adequate food ...

Any research, teaching, or creative activity that involves the use of vertebrate animals must be approved by the Institutional Animal Care and Use Committee (IACUC) prior to ordering animals and prior to commencement of the activity. Applications for such approval may be obtained from the director of the Division of Laboratory Animal Resources (DLAR) or from the Univer-sity coordinator for research compliance. The chairs, deans, and division heads of departments in which laboratory animals are routinely used also have a supply of these applications.. The following is a brief summary of the federal, state, and campus regulations that govern the use of laboratory animals at Stony Brook:. 1. Except as stated in provision 2, all vertebrate animals must be ordered through DLAR. If a University purchase order is unacceptable to the supplier, the DLAR must be so informed in order to determine whether another supplier may be contacted.. 2. The IACUC may waive the requirement of mandatory acquisition of ...

Animal experimentation is a crucial component of scientific research and education. Most advances in modern biomedical science can be traced back to original studies in animals. The university continues to support this critical research while fully appreciating its additional complexities.. All animal research must be approved by the universitys Institutional Animal Care and Use Committee (IACUC), which works close with the Comparative Medicine-Laboratory Animal Facilities (CM-LAF) to help researchers develop effective and compliant protocols. ...

Schulman Associates IRB and Falcon Consulting Group, a provider of Clinical Quality Assurance (CQA) and Good Clinical Practice (GCP) services, have formed a joint venture-Provision Research Compliance Services.. Provision offers global consultancy services for development and implementation of GCP and Human Research Protection (HRP) procedural standards and programs for the conduct of clinical research studies.. There has never been a collaboration of GCP and HRP industry leaders specifically focused on meeting the compliance needs of research institutions around the world, said Michael Woods, Schulman Associates IRB president, CEO and institutional official. Provision makes it easier for institutions to achieve their compliance goals and to engage in global development programs. It also helps study sponsors follow a simpler path to consistent compliance practices across a global network of research institutions.. Provision provides services that combine the GCP quality assurance experience ...

In an effort to increase privacy protections for research participants, as of October 1, 2017, researchers who are collecting identifiable, sensitive information as part of their NIH-funded research will automatically be issued a Certificate of Confidentiality (CoC) as part of the terms and conditions of the award. Researchers conducting NIH-funded research qualifiying for a CoC will no longer need to separately apply for a CoC. Compliance with the privacy protections afforded by the CoC is also a term and condition of the award. All research that was commenced or ongoing on or after December 13, 2016 and is within the scope of this NIH policy was issued a certificate.. Non-NIH funded research that collects identifiable, sensitive information can request a certificate using the Online Certificate of Confidentiality system. Note: the system and process for requesting a CoC was updated and simplified in 2020. The new CoC system requires direct submission by the authorized institutional official, ...

Figure 1 shows the association of date with number of R01 applications received by NIH for consideration in the May 2015 council round, the latest round for which complete award data are available. The primary R01 receipt date for new, unrevised applications was October 6, 2014. NIH received 2,788 applications through October 5, the day before the deadline; an additional 2068, or 43% of the total, came in on the October 6 due date. Even among the applications received before the deadline, the vast majority came in the week before. It may be worth noting that it is an institutional official, usually in the office of sponsored programs that is responsible for submit the applications. So PIs may have completed their application earlier, but may not have built in enough time for the authorized organizational official to process and submit the application earlier.. Perhaps submission patterns are more timely for small business applications. Figure 2 shows corresponding data for Small Business ...

Dr. Lindeman has distinguished herself as a surgical educator. She served as an Administrative Chief Resident at Hopkins and won both the Medical Student Teaching and the Resident Teaching awards. She earned a Masters of Education in the Health Professions from Johns Hopkins University and served as a member of the Board of Directors for the Association of American Medical Colleges for four years. She is also an author of the AAMCs Core Entrustable Professional Activities for Entering Residency. At UAB, she is the Associate Designated Institutional Official for the Clinical Learning Environment and says her desire is to improve the education and experiences of medical students and residents at UAB and across the United States ...

Slice preparation. Neocortical slices were prepared from Sprague Dawley rats (7-11 d old). Animals were handled and housed according to the National Institutes of Health Committee on Laboratory Animal Resources guidelines. All experimental protocols were approved by the University of Alabama at Birmingham Institutional Animal Care and Use Committee. Every effort was made to minimize pain and discomfort. Rats were anesthetized with ketamine and decapitated. The brain was removed quickly and placed in ice-cold artificial CSF (ACSF), which contained the following (in mm): 125 NaCl, 3.5 KCl, 0.5 CaCl2, 3.5 MgCl2, 26 NaHCO3, and 10 d-glucose. The solution was bubbled with 95%O2/5%CO2 to maintain pH at ∼7.4. Coronal brain slices (300 μm thick) containing the anterior cingulate cortex and the shoulder or Fr2 region of the frontal cortex (Paxinos and Watson, 1997) were cut using a vibratome. Slices were stored for 45 min at 37°C and kept at room temperature until recording. The oxygenated storage ...

CRMP family proteins (CRMPs) are widely portrayed in the developing neurons mediating a variety of fundamental functions such as growth cone guidance neuronal polarity and axon elongation. beinteracted with cytoskeleton proteins. Taken collectively we exposed that CRMPs were colocalized with cytoskeleton in hippocampal neurons especially in growth cones. CRMPs can interact with both tubulin and actin therefore mediating neuronal development. by GST-pulldown assay. Materials and methods Animals The experiments were carried out on 1-day-old pups of Sprague-Dawley rats. All animal methods were performed Pracinostat in stringent accordance with the recommendations in the Guidebook for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocol was authorized by the Jinan University or college Institutional Animal Care and Use Committee (IACUC). All attempts were made to minimize the suffering and quantity of animals used. Providers TRIzol reagent and SuperScript VILO ...

Chemicals. MRL-I, [ethyl-14C]MRL-I, MRL-II, [13CD3]MRL-II, and [methyl-14C]MRL-II (Fig. 1) were synthesized at Merck Research Laboratories and were provided as their arginine salts. Alamethicin, saccharolactone, and UDPGA were purchased from Sigma-Aldrich (St. Louis, MO). Acetonitrile and methanol (HPLC grade) were obtained from Fisher Scientific Co. (Pittsburgh, PA). All other reagents were of analytical or HPLC grade.. Animals. Male Sprague-Dawley and obese Zucker rats were obtained from Charles River Laboratories Inc. (Wilmington, MA). They were housed in cages and maintained on a 12-h light/dark cycle. Access to food and water was allowed ad libitum. Rats used in this study were 14 to 16 weeks old; the Sprague-Dawley rats weighed ∼300 g and the obese Zucker rats weighed ∼600 g. All procedures for animal experiments were approved by the Merck Research Laboratories Institutional Animal Care and Use Committee.. Studies in Bile Duct-Cannulated Rats. [14C]MRL-I (1 mg/kg) or [14C]MRL-II (0.5 ...

Dr. Kamal is a Research Associate Professor, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of Florida, Gainesville, FL. He is a Chair, Subcommittee for Research Safety and member of Research and Development committee , North Florida/South Georgia Veterans Health Care System, Malcom Randall VAMC. Also a member of Institutional Animal Care and Use Committee (IACUC) North Florida/South Georgia Veterans Health Care System.

Mice, cell lines, peptides, and dimerizer drug. Six- to eight-week-old female C57BL/6 mice were purchased from the Center for Comparative Medicine at Baylor College of Medicine and Harlan Sprague Dawley Laboratory. CD45.1 C57BL/6 mice, B6.129S1-IL12atm1Jm/J (IL-12p35-deficient), B6.129S1-IL12btm1Jm/J (IL-12p40-deficient), B6.129S1-Ifnγtm1Ts/J (IFN-γ-deficient), and B6.129S4-Cxcl10tm1Adl/J (IP-10-deficient) mice were purchased from the Jackson Laboratory and maintained under pathogen-free conditions in the Transgenic Mouse Facility at Baylor College of Medicine. Animal procedures were approved by the Institutional Animal Care and Use Committee of Baylor College of Medicine. E.G7-OVA, EL-4, YAC-1, HEK293, B16.F10, and THP-1 cells were purchased from ATCC and cultured as recommended by the vendor. Peptides were produced at 80%-95% purity by Genemed Synthesis Inc. Dimerizer drugs, AP1903, and AP20187 were provided by ARIAD Pharmaceuticals. Recombinant plasmids and Ads are described in Supplemental ...

Mice. Experiments were performed using EAAC1+/-, EAAC1-/- (15), GLT-1+/- (17), GLAST +/-, and GLAST-/- (14) mice with approval from the Institutional Animal Care and Use Committee of Tokyo Medical and Dental University and the Tokyo Metropolitan Institute for Neuroscience. All the mice used in this study were backcrossed with C57BL/6 more than 8 times. Immunohistochemistry. Frozen 12-μm-thick retinal sections were incubated with 1 of 4 sets of primary antibody mix. Primary antibodies used were mouse anti-GS (Chemicon International) and rabbit anti-GLAST (13) or anti-glutathione (Signature Immunologics); and mouse anti-calretinin (Chemicon International) and rabbit anti-EAAC1 (49) or anti-GLT-1 (13). Fluorescence immunohistochemistry was performed using Cy2-conjugated donkey anti-mouse IgG and Cy3-conjugated donkey anti-rabbit IgG (Jackson ImmunoResearch Laboratories Inc.). Sections were examined by fluorescence microscopy (Olympus). Histological and morphometric studies. Paraffin sections ...

Recordings were made from adult C57BL/6 mice, 2-6 months of age. The animals were maintained in the animal facility at University of California, San Francisco (UCSF) and used in accordance with protocols approved by the UCSF Institutional Animal Care and Use Committee. For surgery, mice were sedated with an intraperitoneal injection of chlorprothixene (5 mg/kg) and then anesthetized with urethane (0.5-1.0 g/kg, i.p., at 10% w/v in saline). Administration of chlorprothixene several minutes before urethane greatly reduced the dosage of urethane necessary to induce surgical anesthesia. Additionally, atropine (0.3 mg/kg) and dexamethasone (2 mg/kg) were administered subcutaneously to reduce secretions and edema, respectively. The animal was maintained at 37.5°C by a feedback-controlled heating pad. A tracheotomy was performed, and a small glass capillary tube was inserted to maintain a free airway. After retracting the scalp, we performed a small craniotomy, ∼1 mm in diameter, and nicked a slit ...

Institutional Animal Care and Use Committee (IACUC) of the Faculty of Medicine, Chulalongkorn University. Bangkok, Thailand. We divided 14 mice into equalsized sham and Chr IR surgery groups. The baseline blood chemistry tests were conducted 2 weeks before nephrectomy (-2 wk) using blood samples obtained from a tail vein. One week later a Chr IR injury was made as previously described [ 10 ], with slight modifications as follows. In brief, the surgery was conducted in 2 stages. In the first stage at 1 week after baseline blood collection (-1 wk), an abdominal incision was. ...

where C represents the initial substrate concentration in the incubation medium (1 μM).. Animals. Male Wistar rats (220-300 g) (Charles River Laboratories, Inc., Raleigh, NC) were used as liver donors for hepatocyte isolation. Rats were maintained on a 12-h light/12-h dark cycle with free access to water and rodent chow. Rats were allowed to acclimate for at least 5 days before experimentation. The Institutional Animal Care and Use Committee of the University of North Carolina at Chapel Hill approved all procedures.. Transport Studies Using Suspended Primary Rat and Human Hepatocytes. Rat hepatocytes were isolated from male Wistar rats by a two-step collagenase perfusion method as described previously (Liu et al., 1999). Human hepatocytes were isolated in a similar manner, as described above. Isolated hepatocytes were washed twice with cold HBSS modified with 10 mM Tris and 5 mM glucose (+ Na+ condition) or Na+-free choline buffer (10 mM Tris, 5 mM glucose, 5.4 mM KCl, 1.8 mM CaCl2, 0.9 mM ...

Mice. Female homozygous DBA/1j mice (7 weeks old) were obtained from The Jackson Laboratory (Bar Harbor, Maine, USA). All mice were kept in a room equipped with an air-filtering system. The cages, bedding, water, and food were sterilized, and the mice were handled with sterile gloves. All animal procedures were approved by The University of Alabama at Birmingham Institutional Animal Care and Use Committee.. Ad expression vectors for inducible TRAIL and GFP. An adenovirus expressing inducible TRAIL was constructed as described previously (29, 40, 41). Briefly, the full-length TRAIL was first cloned into the BamHI polylinker site of a TRE vector (Clontech, Palo Alto, California, USA) site. The TRE-regulated TRAIL fragment, including the bovine growth hormone poly-A tail, was then excised with XhoI and HindIII, followed by insertion into the Klenow-filled NotI site of the pShuttleCMV, leading to the production of pShuttleTRAIL. The recombinant Ad AdTRAIL was produced by in vitro recombination of ...

Selective modulation of α7 nicotinic acetylcholine receptors (nAChRs) is thought to regulate processes impaired in schizophrenia, Alzheimers disease, and other dementias. One approach to target α7 nAChRs is by positive allosteric modulation. Structurally diverse compounds, including PNU-120596, 4-naphthalene-1-yl-3a,4,5,9b-tetrahydro-3-H-cyclopenta[c]quinoline-8-sulfonic acid amide (TQS), and 5-hydroxyindole (5-HI) have been identified as positive allosteric modulators (PAMs), but their receptor interactions and pharmacological profiles remain to be fully elucidated. In this study, we investigated interactions of these compounds at human α7 nAChRs, expressed in Xenopus laevis oocytes, along with genistein, a tyrosine kinase inhibitor. Genistein was found to function as a PAM. Two types of PAM profiles were observed. 5-HI and genistein predominantly affected the apparent peak current (type I) whereas PNU-120596 and TQS increased the apparent peak current and evoked a distinct weakly decaying ...

In October 2016, the Ministry for Primary Industries began an investigation into allegations of breaches of the Animal Welfare Act 1999 at the Animal Control Unit of the Napier City Council.. The allegations related to the period between August 2015 and October 2016. The investigation was concluded in January 2017 and a report released in May 2017.. The report concluded there was either no or insufficient evidence of breaches of the Animal Welfare Act 1999.. ...

USDA touches the lives of all Americans each day in so many positive ways. In the Biden-Harris Administration, USDA is transforming Americas food system with a greater focus on more resilient local and regional food production, fairer markets for all producers, ensuring access to safe, healthy and nutritious food in all communities, building new markets and streams of income for farmers and producers using climate smart food and forestry practices, making historic investments in infrastructure and clean energy capabilities in rural America, and committing to equity across the Department by removing systemic barriers and building a workforce more representative of America. To learn more, visit www.usda.gov ...

Amendments to the Animal Welfare Act 2006 will become UK law from 6th April 2016. Following a successful campaign, and no doubt much lobbying by both the Kennel Cub and Petlog, new regulations have been passed through Parliament which address the urgent need to improve responsible dog ownership. Within the secondary legislation under the Animal Welfare Act 2006, from 6th April 2016, microchipping will be mandatory for all dogs in England: ...

Lab animals are helping us unlock the mysteries of disease and deserve our respect. The US Government closely regulates the care of animals in research.

This guidance is intended to help sponsors determine the amount and types of safety data to collect during late-stage premarket and postapproval clinical investigations, (e.g., phase 3 clinical trials, studies of new uses, long-term outcomes).

Depending on the agent to be used, the committee with oversight of the use of that agent will require a completed internal form, an informative abstract of the work to be done and a standard operating procedure for the use of the agent in animals. In the standard operating procedures consideration should be given to the amount of hazardous agent to be used, its toxic level for both animals and humans (LD50) and its metabolism, if involved, so that decisions can be made about its presence in excreta and bedding that require special handling. The speed of the approval process is directly related to the completeness of the abstract and the standard operating procedures.. The use of hazardous chemicals including carcinogens, teratogens and toxins, is reviewed by the Laboratory Safety Committee (internal form RO-14).. The use of biohazardous etiological agents, recombinant DNA, as well as human blood, tissues, body fluids, and primary cells or cell lines are reviewed by the Institutional Biosafety ...

Every Single Flu Vaccine Myth, DebunkedWhy fear is trumping science when it comes to EbolaThe Mythology Of Science-Based Medicine so much wrong in so few paragraphs.The Ebola Crisis & the Homeopathic CommunityTHE EVIDENCE INFORMED CHIROPRACT

The 3Rs: Replacement, Reduction, and Refinement, are important from a legal, ethical and scientific standpoint. All research using animals in the US, for example at universities and pharmaceutical companies, is regulated by the Animal Welfare Act (AWA), which is administered by the Animal and Plant Health Inspection Service (APHIS) of the US Department of Agriculture…

The URMC clinical laboratories provide routine, stat, and a broad range of specialized testing services to support the needs of primary care physicians and specialists alike. We are committed to providing the best laboratory service in the region and to staying abreast of the latest innovations in Pathology and Laboratory Medicine.. As a part of our ongoing efforts to work together in providing optimal laboratory service and meeting all compliance regulations, we share several important recommendations.. ...

Male SHR and normotensive WKY rats were obtained from Charles River Breeding Laboratories Inc (Wilmington, Mass) at 14 weeks of age and were maintained thereafter in accordance with our institutional animal care committee guidelines after prior approval of the experimental protocol. They were housed in temperature-controlled rooms on a 12-hour light/dark cycle and were fed usual rat chow and water ad libitum. At 23 weeks of age, the rats were randomly assigned to one of five groups of 16 rats each: untreated normotensive WKY-C (tap water), untreated SHR-C (tap water), SHR-E (30 mg·kg−1·d−1 by gavage), SHR-L (30 mg·kg−1· d−1 by gavage), and SHR-E&L (5 mg·kg−1 ·d−1 of each agent by gavage). The rats followed their respective treatments for 12 weeks. All rats were trained to run on a motor-driven treadmill (Columbus Instruments) at speeds ranging from 0.5 to 1.0 m/min, 0% grade, for ≈5 min/d for 2 to 3 days a week during the final 2 weeks of the protocol to familiarize them with ...

Alternative therapies common among kids, study finds Proposed legislation integrates chiropractic physicians into public health service . "A newly proposed bill, H.R. 2851, in the U.S. House of Representatives calls for more incorporation of doctors

As I pointed out earlier, a rare thing happened this week, namely I dont have a full post ready for Science-Based Medicine because Im at the Lorne Trottier Symposium. Not only have the organizers have packed my day with skeptical and science goodness, but I only have Internet access when Im back at the hotel, which isnt very often. I suppose I.... ...

Heres an informative and well done skeptical comic from those geniuses over at PhD comics: We can glean a number of fascinating things from this comic. First, I bet you didnt know where the term mesmerizing came from. Youre welcome. Second, while medical professionals who practice science-based medicine would argue that the placebo effect is…

IFIC Foundation would like to congratulate the European Food Information Council (EUFIC), which is celebrating its 20th Anniversary this year! Similar to IFIC in its mission, EUFIC provides balanced and science-based information on nutrition and health, food safety and quality, and consumer insights in Europe.

The holiday season is upon us. As a bit of a holiday from science-based writing, I thought I would offer some thoughts inspired by the season and not supported by any scientific evidence. One of my friends refers to Christmas as The Feast of St. Dyspepsia. Holidays are indeed an occasion for over-indulging. People change their routine: they have time off work,.... ...

Brewer Heineken has today (12 February) unveiled a new sustainability strategy that commits to ramping up renewable energy production, piloting an internal carbon price and setting science-based targets across distribution, cooling and packaging. - edie news centre

Return to Breeding Rules. Breeders are reminded that there is a 6 LITTER MAXIMUM. Those breeders trying to register more than the maximum number of litters will be required to provide a genuine reason in writing to the Stud Book Committee. Litters will not be registered until after the Stud Book Committee have considered the reasons given.. Three litters maximum in three years is a principle that the ISDS requires from its breeders in order to demonstrate compliance with the obligations of the 2006 Animal Welfare Act. This act specifically limits registered breeders to one litter per year.. Many ISDS breeders however are not registered, so although the law does not strictly apply the ISDS still accepts the welfare principles. We will therefore allow three litters in two years, plus a years rest, in the rare case that a bitch is mated unwittingly and too soon on the third occasion; but we then insist on a years rest and there have been issues when this rest period of one year has been ...

The Animal Welfare Act was signed into law in 1966, originally to protect laboratory animals while also regulating animal dealers and the laboratories…. ...

The numbers are not that high of what you would expect from puppy mill operations like we all have seen in the news. It is on a much smaller scale. But the practices are the same. Breed w/o taking health and well-being into account for a profit. When they made the news the first time - now 10 years ago - the police paid them a couple of visits. Unfortunately they could only fine them. And since such a fine is lower than the price of a puppy.... No, the Danish Animal Welfare Act is not equipped for this. A couple filed an official consumer complaint after problems with their puppies, but again, this only forces them to refund the amount paid for the puppy, and doesnt stop them.. Delete ...

Buy Methods of collecting and preserving vertebrate animals by Rudolph Martin Anderson online at Alibris. We have new and used copies available, in 2 editions - starting at $0.99. Shop now.

The newly reassembled DMNA Land Use Committee will meet on the first Wednesday of each month at 6:00 pm at a rotating location. This months meeting will be held in the Emanuel Housing board room at 818 3rd Street S. All are welcome.. A proposed hotel development at 903 Washington Ave. is the featured topic. The developer/owner will provide an overview of the project and collect feedback. Please review the Stone Arch Hotel - DMNA Submittal 10.1.2015 for more information.. Contact Joan Bennett, DMNA Program & Outreach Coordinator, with questions. [email protected]. ...

Subjects. Twelve DBA/2J male mice, 13 DBA/2J female mice, 12 C57BL/6J male mice, and 12 C57BL/6J female mice were obtained from The Jackson Laboratories (Bar Harbor, ME). The animals were 9 to 10 weeks old at the beginning of discrimination training and were individually housed on a 12-h light/12-h dark cycle. The mice were weighed and allowed to acclimate to the laboratory for 2 weeks before training began. All mice were fed enough rodent chow (Harlan, Indianapolis, IN) at least 1 h postsession to maintain a body weight between 20 and 30 g. The Wake Forest University School of Medicine Animal Care and Use Committee approved all procedures involving these mice and procedures were conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals.. Apparatus. Drug discrimination sessions took place in standard two lever mouse operant-conditioning chambers (MED Associates, St. Albans, VT). Each chamber was equipped with two levers on the front wall of ...

Definition of Indian Child Welfare Act in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is Indian Child Welfare Act? Meaning of Indian Child Welfare Act as a legal term. What does Indian Child Welfare Act mean in law?

Research Regulatory Committees An Overview of the Institutional Review Board (IRB) Presented by Rowan University Foundation / University Advancement and Office of Sponsored Programs April 22, 2014 OFFICE OF RESEARCH ROWAN UNIVERSITY Provide assistance and guidance to investigators to ensure ethical conduct and compliance in performance of research in the following areas:  Human subjects protection  Animal care and use  Use of hazardous agents, radioactive materials and recombinant DNA material  Responsible conduct of research (Ethics and Integrity) IRB Organization Structure  MANAGEMENT: Institutional official and IRB director  GOALS:    Centralized single Human Subjects Protections Administrator (HSPA) for both institutions Centralized compliance office for all regulated research Management of institutions sponsored programs are maintained directed by Vice President for Research Human Subject Protection Program Mission and Services  To protect the rights, ...

The Acting Administrator of the Animal and Plant Health Inspection Service (hereinafter Complainant) instituted this disciplinary administrative proceeding under the Animal Welfare Act, as amended (7 U.S.C. §§ 2131-2159) (hereinafter the Animal Welfare Act); the regulations and standards issued under the Animal Welfare Act (9 C.F.R. §§ 1.1-3.142) (hereinafter the Regulations and Standards); and the Rules of Practice Governing Formal Adjudicatory Proceedings Instituted by the Secretary (7 C.F.R. §§ 1.130- .151) (hereinafter the Rules of Practice), by filing a Complaint on July 6, 1994. On September 2, 1994, Respondents Counsel, Christopher M. Patterson, Esq., filed an Answer to the Complaint on behalf of Respondent. On December 11, 1995, Respondents Counsel filed a Motion to Withdraw Appearance, which motion Administrative Law Judge Edwin S. Bernstein (hereinafter ALJ) granted on December 18, 1995.. On December 21, 1995, Complainant filed an Amended Complaint which includes counts I-VI ...

JAIME GATENO, M.D., D.D.S. Dr. Gateno, chairman of the Department of Oral and Maxillofacial Surgery at The Methodist Hospital, was one of six surgeons worldwide selected to represent a Craniofacial Expert Group for the AO Foundation, a medically guided nonprofit organization specializing in the treatment of trauma and disorders of the musculoskeletal system. Gateno has an international reputation for his expertise in craniofacial and orthognathic surgery. JUDY PAUKERT, Ph.D. Dr. Paukert, vice president of The Methodist Hospital Education Institute, was honored with the Parker J. Palmer Courage to Lead Award at the annual meeting of the Accreditation Council for Graduate Medical Education (ACGME) in 2012. Paukert is Methodists designated institutional official for the ACGME and for the hospitals Graduate Medical Education Committee sponsored programs. In just seven years, Paukert has achieved successful accreditation of 25 ACGME programs and transitioned five others from an institution that no ...

This workshop introduces academics to the grants world. It gives beginners a framework for understanding this complex world and advice about how best to expend their energies to gain the maximum return from their efforts.. Register today!. TRCCC Annual Meeting: The Power of Negative Thinking: Inhibiting the Inhibitors of Tumor Immunity. Registration is open for the 16th Annual Meeting of the Translational Research Cancer Centers Consortium (TRCCC),The Power of Negative Thinking: Inhibiting the Inhibitors of Tumor Immunity. Held February 20-22 at the Seven Springs Mountain Resort in Seven Springs, PA, this meeting will feature two symposiums focused on Tumor-mediated Immune Suppression and Vaccine and Cellular Therapy, with spotlight sessions on Imaging and Viral Vectors. The agenda will also include a poster session. Researchers, institutional officials, and students are invited to submit abstracts for poster presentations by February 4.. ...

Animal welfare science is the scientific study of the welfare of animals as pets, in zoos, laboratories, on farms and in the wild. Although animal welfare has been of great concern for many thousands of years in religion and culture, the investigation of animal welfare using rigorous scientific methods is a relatively recent development. The worlds first Professor of Animal Welfare Science, Donald Broom, was appointed by Cambridge University (UK) in 1986. Early legislation which formed the impetus for assessing animal welfare and the subsequent development of animal welfare science include the Ireland Parliament (Thomas Wentworth) An Act against Plowing by the Tayle, and pulling the Wooll off living Sheep, 1635, and the Massachusetts Colony (Nathaniel Ward) Off the Bruite Creatures Liberty 92 and 93 in the Massachusetts Body of Liberties of 1641. Richard Martins act, the Cruel Treatment of Cattle Act 1822 is often considered to be the precursor of modern relevant legislation. One of ...

All Beagle dog studies were performed by CiToxLAB North America (Canada) and conducted in accordance with their institutional animal care and use committee and the principles outlined in the Guide for the Care and Use of Laboratory Animals from the National Institutes of Health. Dogs (n = 14 dogs; 7 males and 7 females; age: 8 to 9 months at the time of ABP-700 dosing) were purchased from Marshall BioResources (USA). Dogs were individually housed in stainless steel cages equipped for telemetry data acquisition and day/night vision cameras for continuous video monitoring. Room temperature was maintained at 21° ± 3°C with 12-h light-dark cycles. At least 26 days before ABP-700 dosing, dogs were anesthetized with propofol and isoflurane and surgically instrumented for telemetric monitoring of electroencephalography and electromyography, intraarterial blood pressure, heart rate, body temperature, and physical activity generally as previously described.21 Sterile transmitters (D70-EEE and M10; ...

This animal study was approved by the Institutional Animal Care and Use Committee of Case Western Reserve University and was conducted in accordance with the Guide for the Care and Use of Laboratory Animals (NIH publication No. 86-23, revised 1985). The adult male Charles River guinea pigs were anesthetized with intraperitoneal ketamine (54 mg/kg), acepromazine (1.8 mg/kg), and xylazine (10.9 mg/kg) and heparinized. Hearts were quickly excised and perfused by the Langendorff method with a modified Krebs-Henseleit buffer containing (in mmol/L) NaCl 113.8, KCl 4.7, MgSO4 1.1, KH2PO4 0.12, NaHCO3 23.6, CaCl2 2.5, mannitol 6.0, and glucose 11.0, as previously reported.5 18 The solution was saturated with 95% O2/5% CO2 (pH 7.4) at 37°C. A saline-filled latex balloon attached to a 3F micromanometer catheter (Millar Instruments) was inserted into the left ventricle through the mitral valve for pressure measurements.5 18 The balloon was inflated to achieve 10 mm Hg initial minimum diastolic pressure ...

Last updated November 3, 2017. Statement of Human and Animal Rights. For research studies using human or animal subjects, the trials design, conduct and reporting of results must conform to Good Clinical Practice guidelines (such as the Good Clinical Practice in Food and Drug Administration (FDA)-Regulated Clinical Trials (USA) or the Medical Research Council Guidelines for Good Clinical Practice in Clinical Trials (UK)) and/or to the World Medical Association (WMA) Declaration of Helsinki. Generally, we suggest that the national standard of the lead investigator be followed. If authors have any doubt as to whether the research was conducted in accordance with the above standards, the rationale for the chosen experimental approach must be clearly presented, along with a statement and proof of explicit approval given by the appropriate Institutional Review Board (IRB, for human subjects) and/or the Institutional Animal Care and Use Committee (IACUC, for animal subjects) for conducting the ...

These data indicated that inactivation of 1 1 allele was adequate to augment T cellClineage commitment from restores DN3-like cell development from or (D) and mRNA isolated from sorted DN1-like (white), DN2-like (gray), or DN3-like (black) cells isolated from your cultures shown in panel A. nonCT-cell progenitors in vivo. We propose a model in which the overproduction of GATA3 stretches the self-renewal capacity of DN2 cells and inhibits T lineage restriction, whereas E2A deficiency, independent of the overproduction of GATA3, allows DN2 cells to access alternative fates. Therefore, T-lymphocyte commitment requires passage through a checkpoint in DN2 cells in which positive and negative inputs to must be balanced to allow further differentiation. Methods Mice and genotyping This work was authorized by the Institutional Animal Care and Use Committee in the University or college of Chicago under protocol ACUP 71 119. Mice were housed in the University or college of Chicago Animal Resource Center. ...

Purpose Glial-derived neurotrophic factor (GDNF) is definitely a potential therapy for stroke, Parkinsons disease, or drug addiction. high clearance rates of 8C10?mL/kg/min. Conclusions A no-observable-adverse-effect level is established in the Rhesus monkey for the acute administration of the HIRMAb-GDNF fusion protein. The fusion protein targeting the insulin receptor has a PK profile similar to a classical small molecule. and were approved by the Institutional Animal Care and Use Committee. Study Design Dose Selection The animals were treated with either 0, 0.4, 2, or 10?mg/kg of the HIRMAb-GDNF fusion protein administered as a slow bolus intravenous injection over a 2-min period. In the 2-week toxicity study, the doses were Rabbit Polyclonal to CDK1/CDC2 (phospho-Thr14). administered every 12?h for SRT1720 HCl five consecutive doses over a 60-hr period, with a total dosing of 0, 2, 10, and 50?mg/kg. 2-Week Toxicity Study The initial clinical application of the HIRMAb-GDNF fusion protein is ...

Lyndsay Cole (970) 494-7410. WASHINGTON, Dec. 28, 2012--The U.S. Department of Agricultures (USDA) Animal and Plant Health Inspection Service (APHIS) is taking public comments on the Agencys intention to use the 2010 Guidelines for the Control of Tuberculosis in Elephants, issued by the United States Animal Health Association (USAHA), to assess whether or not USDA licensees are adhering to the Animal Welfare Act (AWA) regulations and providing their elephants with adequate veterinary care with respect to tuberculosis (TB), TB testing and elephant movement.. Ensuring that elephants covered under the Animal Welfare Act are receiving adequate veterinary care and protecting those individuals and members of the public who may come in contact with these animals is a top priority, said APHIS Acting Administrator Kevin Shea. APHIS has long used USAHA guidelines on TB testing and elephant movement and we are seeking comment on how best to incorporate the 2010 guidance into our enforcement.. TB is ...

animal welfare News: Find latest news, video & photos on animal welfare. Explore all information & updates about animal welfare online at tamil.asianetnews.com

Material Transfer Agreements (MTAs): MTAs are agreements that govern the transfer of various biological and research materials. An MTA must be signed by an authorized signatory of the College before any material is transferred. If you are interested in receiving materials from another institution, a nonprofit, or a for-profit organization and have received a draft MTA or are interested in initiating the process, please contact SPARC. SPARC will review and work with appropriate constituencies to facilitate material transfers. ...

An important component of post-surgical care is observation of the animal and documentation of its recovery from anesthesia and major surgery. Major surgery penetrates and exposes a body cavity, involves orthopedic surgery, or produces significant impairment of physiological function. The intensity and type of monitoring necessary will vary with the species and the procedure and might be greater during the immediate anesthetic recovery period than later in postoperative recovery.. Documentation of post-surgical recovery is required for USDA covered species and recommended for all vertebrate species. USDA covered species includes dogs, cats, nonhuman primates, guinea pig, hamster, rabbit, farm animal, or any other warm-blooded animal (* Members of the Genus Mus and Rattus are currently exempt from USDA coverage).. During the anesthetic recovery period, the animal should be in a clean, dry area where it can be observed as appropriate, and as specified in the approved protocol. Particular attention ...

Characterization of the mice studied. Donor cells and platelets were derived from C57BL/6 WT mice (The Jackson Laboratory) or mUK-transgenic mice, which ectopically express murine urokinase within megakaryocytes (14). Recipient mice were homozygously transgenic for hαIIb and null for the expression of platelet mouse αIIb (mαIIb-/-) (8), designated hαIIb+ mice, and expressed 20% of the level of CD41 seen on human platelets (12). All animal studies were done with approval of the Institutional Animal Utilization Committee at the Childrens Hospital of Philadelphia. Isolation of platelets and megakaryocytes ex vivo. FL megakaryocytes were obtained from E14 FL cells homogenized and cultured as previously described (7). Adult BM cells were obtained from femurs and tibiae of C57BL/6 mice (21). Mature megakaryocytes were isolated using a 2-step density gradient (21). Washed platelets derived from the inferior vena cava of C57BL/6 mice in acid-citrate-dextrose were prepared as previously described ...

Since the end of the Australian Animal Welfare Strategy (AAWS) in 2014, there has not been an overarching mechanism for national coordination, collaboration, and engagement on animal welfare for all animals. TAWC operates in a similar spirit to the AAWS but is facilitated by academics across multiple Australian universities rather than by the Federal Government. TAWC facilitates information exchange and national conversations about animal welfare and provides a mechanism for various groups to drive on-the-ground practice change.. ...

German animal welfare association speaks out against rabbit fattening farms from these include mainly animal shelters, grace farms and short-term actions that are quick and unbureaucratic assistance. Since the inception of the animal welfare Alliance rabbit mast, no, thank youof the German animal aid association is a partner of the first hour, which actively supported the Alliance. We are pleased to have an important allies in the fight against the rabbit mast on our side with the German animal welfare association, as Ingo Schulz, spokesman of the Alliance. The newspapers mentioned mayo clinic not as a source, but as a related topic. Under the name rabbit fattening, no, thank youwell-known animal protection organizations in the fight against the rabbit mast have joined forces. The Alliance gives a look behind the scenes of the brutal reality of the rabbit production in the industrial factory farming, thereby reveals how trade groups and lobbyists try to hide the cruelty behind a shiny ...

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Title:Science-based Ethnic Bridging in Drug Development; Review of Recent Precedence and Suggested Steps Forward. VOLUME: 14 ISSUE: 3. Author(s):Ewoud-Jan van Hoogdalem*, John P. Jones III, John Constant and Meguru Achira. Affiliation:PRA Health Sciences, Scientific Affairs - Clinical Pharmacology, Groningen, PRA Health Sciences, Scientific Affairs - Clinical Pharmacology, Blue Bell, PA, PRA Health Sciences, Scientific Affairs, Victoria, BC, Clinical Pharmacology, Takeda PRA Development Center KK, Osaka 540-8645. Keywords:Race, ethnicity, product label, clinical drug development, tacrolimus, antihypertensives.. Abstract:Background: Exposure, safety and/or efficacy of drugs are subject to potential differences between human races or ethnicities, as acknowledged by regulatory guidance and by label texts of various, but not all approved drugs. Objective: The objective of the present review was to assess recent regulatory precedence on drug use and race or ethnicity, with the goal of identifying ...

Coffee enemas are popular in the natural health community. This article takes a critical, science-based look at the use of coffee enemas for health.

The same is true of web-hosting; you want a massive globe-girdling operation that has balck facilities, staff and sheer scale to keep your site running responsively under the terminal services client windows 2000 server download adverse conditions. We want you to be satisfied and we go to great lengths to make our customers mars-1 observer black. This is because important information can potentially be lost in the transition jars-1. If youve exceeded the power offered by a shared hosting plan, virtual private server (VPS) hosting will arm you with increased performance without saddling you with the full cost of a dedicated server. I hope this review helps. Research was obsserver in compliance with the Animal Welfare Act and other federal vncserver geometry command and regulations relating to animals and experiments involving animals and adhered to principles stated in the Guide for the Care and Use of Laboratory Animals 15 The facility where this research was conducted is fully accredited by ...

In order to obtain peritoneal macrophages, Balb/C mouse strain males, age 8 to 12 weeks, were injected i.p. with 300 fig of zymosan (SIGMA) dissolved in a phosphate buffer (PBS) in a total volume of 0.1 ml/mouse. After 24 hours the mice were euthanized according to the Laboratory Animal Welfare Act. The peritoneal cavity was washed with a sterile physiological solution (5 ml). The obtained peritoneal macrophages were washed twice with a sterile physiological solution and, after the last centrifugation (350 g/10 min), resuspended in RPMI 1640, into which 10% of FBS were added. In order to determine TNF-a secretion, SxlO4 cells/well were cultivated in a total volume of 200 \x\ for 18 to 24 hours on midrotitre plates with a flat bottom (96 wells. Falcon) in RPMI 1640 medium, into which 10% FBS (Fetal Bovine Serum, Biowhittaker) inactivated by heat, 100 units/ml of penicillin, 100 mg/ml of streptomycin, 20 mM HEPES and 50 μM 2-mercaptoethanol (all of GIBCO) were added. The cells were incubated at ...

By Lisa Franzetta. For many people faced with a legal situation involving a companion animal, it comes as a shock to discover that the laws protecting their families, neighbors, and friends from violence and abuse fail to protect their four-legged loved ones. Despite the important role they play in so many of our lives, animals in this country are still considered property by law-in most cases no different than a table or a chair. Existing laws are often inadequate, full of loopholes, or lack enforcement. There are still states where no matter how badly someone abuses or tortures an animal, he cannot be charged with a felony. The federal Animal Welfare Act explicitly excludes birds, rats, and mice, and there are no federal laws protecting the billions of animals being raised for food from the most egregious abuses on factory farms.. For 30 years, the Animal Legal Defense Fund (ALDF) has been fighting to protect the lives and advance the interests of animals through the legal system. This ...

Every year, tens of millions of animals are used in cruel and deadly taxpayer-funded experiments inside university laboratories across the country. Now, Every year, tens of millions of animals are used in cruel and deadly taxpayer-funded experiments inside university laboratories across the country. Now, PETA-recognizing that all experimentation on animals is cruel and unethical-has created Failed Tests, an interactive feature that ranks colleges as bad, worse, or worst for animals. The grades are based on the number of animals covered by the federal Animal Welfare Act (AWA)-including pigs, dogs, cats, rabbits, and monkeys-who are held and used in experiments at the school, the amount of government funding received from the National Institutes of Health, and the number and severity of a schools AWA violations.

The cat is the most popular companion animal, and people are increasingly interested in its wellbeing. One of the topics under international debate is the weaning age, i.e., the age at which kittens are separated from their mother and siblings and brought to a new home. In Finland, the recommended minimum age of weaning is 12 weeks, but in many other countries, such as the United States, weaning of kittens as young as 8 weeks is common.. It has previously been thought that the critical period of socialisation in cats ends by 8 weeks of age, after which social experiences have little impact on behaviour.. We found that positive changes in the cats behaviour can occur after the currently recommended age of weaning, 12 weeks. Im a cat lover myself, and this study supports my own previous experiences on the importance of the weaning age on the wellbeing of cats. I think raising the recommended age of weaning would be the animal welfare act of the year, says doctoral student Milla Ahola.. While ...

Buy Clonality: The Genetics, Ecology, and Evolution of Sexual Abstinence in Vertebrate Animals (9780195369670): NHBS - John C Avise, Oxford University Press USA

Developed in response to industry demand, an animal welfare course where you will learn the about the theory and practice of animal welfare - wildlife, pets, farm animals.

Ein, and the total reperfusion time was 150 min; (4) IR treated with RvD1 (IR-RV) group: after blocking the hilum of left lung for 45 min, reperfusion for 10 min then injection 100 g/kg RvD1 by formal vein and the total reperfusion time was 150 min.Blood and tissue harvestThe animal procedures were approved by Wenzhou Medical University Animal Care and Use Committee, which were certified by the Chinese Association of Accreditation of Laboratory Animal Care and were consistent with the Guide for the Care and Use of Laboratory Animals [updated (2011) version of the NIH guidelines]. Male Sprague awley (SD) rats (8 weeks old) were fed a standard diet and maintained in the controlled environment of the animal center at 25 ? 1 under a 12 h light ark cycle. The LIRI rat model was induced by the following procedures. Briefly, rats were anesthetized by an intraperitoneal injection of 10 chloral hydrate (300 mg/kg body weight) and placed in a supine position. The animals were then intubated for artificial ...

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The Animal Welfare Society exists to provide humane shelter and care to animals and to further the cause of responsible animal adoption and ownership.

Catalog Use Committee/Discover Services Committee. The RSS Board recently approved a name change to the Discovery Services Committee with this revised charge:. The RSS Discovery Services Committee focuses on the use of catalogs and other discovery tools in reference service, including such aspects as communicating, evaluating, improving, studying, and teaching the use of such tools; monitors and communicates significant continuities and changes in the use of such tools; identifies and examines critical issues and problems in the use of such tools; provides opportunities for librarians to propose and discuss innovative solutions to discovery problems; joins with librarians in other specialties to produce improvements in library service.. The Committee will hold a discussion forum at the ALA Annual Conference in Las Vegas on Sunday, June 29 from 3:00-4:00 PM, opening with a few brief presentations as discussion starters.. Colleen Seale, Chair 2013-2014. Communication and Teaching at the Point of ...