To test the hypothesis that the concentration of angiopoietin-2 relative to angiopoietin-1 may be a useful biological marker of mortality in acute lung injury patients. We also tested the association of concentration of angiopoietin-2 relative to ang

Angiopoietin-1/Tek signaling is a critical regulator of blood vessel development, with conventional knockout of angiopoietin-1 or Tek in mice being embryonically lethal due to vascular defects. In addition, angiopoietin-1 is thought to be required for the stability of mature vessels. Using a Cre-Lox conditional gene targeting approach, we have studied the role of angiopoietin-1 in embryonic and adult vasculature. We report here that angiopoietin-1 is critical for regulating both the number and diameter of developing vessels but is not required for pericyte recruitment. Cardiac-specific knockout of angiopoietin-1 reproduced the phenotype of the conventional knockout, demonstrating that the early vascular abnormalities arise from flow-dependent defects. Strikingly, deletion in the entire embryo after day E13.5 produced no immediate vascular phenotype. However, when combined with injury or microvascular stress, angiopoietin-1 deficiency resulted in profound organ damage, accelerated angiogenesis, ...

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TY - JOUR. T1 - Systemic analysis of tyrosine phosphorylated proteins in angiopoietin-1 induced signaling pathway of endothelial cells. AU - Kim, Young-Mee. AU - Seo, Jawon. AU - Yung, Hee Kim. AU - Jeong, Jaeho. AU - Hye, Joon Joo. AU - Lee, Dong Hee. AU - Gou, Young Koh. AU - Lee, Kong Joo. PY - 2007/8/1. Y1 - 2007/8/1. N2 - Angiogenesis is an essential process in physiological and pathological processes and is well-regulated to maintain the cellular homeostasis by balancing the endothelial cells in proliferation and apoptosis. Angiopoietin-1 (Ang1) regulates angiogenesis as a ligand of Tie 2 receptor tyrosine kinase. However, the regulation pathways are not well-understood. To date, only a few of the signaling molecules involved in the Tie 2 receptor tyrosine kinase-mediated angiogenesis have been identified. In this study, we systematically identified tyrosine-phosphorylated proteins in Ang1-induced signaling cascade in human umbilical vein endothelial cells (HUVECs), employing proteomic ...

TY - JOUR. T1 - Angiopoietin-1 protects endothelial cells from hypoxia-induced apoptosis via inhibition of phosphatase and tensin homologue deleted from chromosome ten. AU - Lee, Sae Won. AU - Youn, Seock Won. AU - Kim, Tae Youn. AU - Suh, Jung Won. AU - Koh, Gou Young. AU - Kwon, Yoo Wook. AU - Chae, In Ho. AU - Park, Young Bae. AU - Kim, Hyo Soo. PY - 2009/2. Y1 - 2009/2. N2 - Background and Objectives: Angiopoietin-1 (Ang1) is a regulator of blood vessel growth and maturation, and prevents radiation-induced or serum deprivation-induced apoptosis. Phosphatase and tensin homologue deleted from chromosome ten (PTEN), a well-known tumor suppressor, regulates cell cycle arrest and apoptosis. Hypoxia induces apoptosis by increasing the expression of PTEN. We hypothesized that Angl may regulate PTEN expression and, thus, reduce endothelial apoptosis under hypoxia in vitro and in vivo. Materials and Methods: In vitro, human umbilical vein endothelial cells (HUVECs) were treated with Ang1, and ...

The angiopoietin/Tie (ANG/Tie) receptor system controls developmental and tumor angiogenesis, inflammatory vascular remodeling, and vessel leakage. ANG1 is a Tie2 agonist that promotes vascular stabilization in inflammation and sepsis, whereas ANG2 is a context-dependent Tie2 agonist or antagonist. A limited understanding of ANG signaling mechanisms and the orphan receptor Tie1 has hindered development of ANG/Tie-targeted therapeutics. Here, we determined that both ANG1 and ANG2 binding to Tie2 increases Tie1-Tie2 interactions in a β1 integrin-dependent manner and that Tie1 regulates ANG-induced Tie2 trafficking in endothelial cells. Endothelial Tie1 was essential for the agonist activity of ANG1 and autocrine ANG2. Deletion of endothelial ...

Isolation of angiopoietin-1, a ligand for the TIE2 receptor, by secretion-trap expression cloning. The anticoagulation factor protein S and its relative, Gas6, are ligands for the Tyro 3/Axl family of receptor tyrosine kinases

TY - JOUR. T1 - Angiopoietin-2, permeability oedema, occurrence and severity of ALI/ARDS in septic and non-septic critically ill patients. AU - van der Heijden, M.. AU - van Nieuw Amerongen, G.P.. AU - Koolwijk, P.. AU - van Hinsbergh, V.W.M.. AU - Groeneveld, A.B.J.. PY - 2008. Y1 - 2008. U2 - 10.1136/thx.2007.087387. DO - 10.1136/thx.2007.087387. M3 - Article. C2 - 18559364. VL - 63. SP - 903. EP - 909. JO - Thorax. JF - Thorax. SN - 0040-6376. IS - 10. ER - ...

Effects of Protein and Gene Transfer of the Angiopoietin-1 Fibrinogen-like Receptor-binding Domain on Endothelial and Vessel ...

The results of the present study indicate that TAK1 acts as an AMPKα1 kinase in endothelial cells and positively regulates VEGF-induced, TNF-α-induced, and IL-1β-induced angiogenesis by increasing the expression of the antioxidative mitochondrial enzyme, SOD2. Indeed, superoxide production and the mitochondrial membrane potential were reduced in the absence of TAK1, and the impaired endothelial cell sprouting from aortic rings from TAKΔEC and AMPKα1ΔEC mice was rescued in the presence of PEG-SOD.. The global deletion of TAK1 impairs embryonic development in C. elegans,23 zebrafish, and mice.13 Both the global and endothelial-specific deletions of TAK1 result in vascular malformation, dilated capillaries, and defects in vascular stabilization,14 and correlate well with the phenotype of endothelial cell-specific TGF-β type II receptor conditional mutants.24 In addition to its role in vascular development, TAK1 has also been implicated in the TGF-β-induced regulation of matrix ...

In the circulatory system, while larger features of the vasculature such as arteries and veins appear to be statically positioned for years of use, finer features in the human body and in biology relates to a continuous branching out of a circulatory network that is required for tissue growth. This process is related to biochemical pathways and is vital to be understood so that issues in health and disease can be better addressed. Angiogenesis is the formation of new blood vessels from existing vessels. A functional vascular network is established by balanced angiogenesis processes. While the activation of angiogenesis extends vascular networks crucial for tissue growth, angiogenic vessels should be stabilized to undertake necessary vascular functions. The Notch pathway is fundamental for allowing for vascular stabilization. It acts by preventing excessive angiogenesis. However, how this signaling pathway that is conserved in various biological processes can induce changes that are ...

In this study we found that: (1) LS inhibits tubule formation in HUVECs and HMECs, but not BAECs, however CM from laminar sheared BAECs can inhibit tubule formation of HUVECs, (2) Preconditioning with LS inhibits migration in HUVECs and BAECs, whereas OS does not inhibit migration and this is mediated through secreted protein, (3) Ang2 is downregulated by LS both at the gene and protein level in vitro, (4) Ang2 is downregulated at sites of LS and upregulated at sites of OS in vivo, (5) Knockdown of Ang2 inhibits OS-mediated tubule formation and migration, and (6) Addition of recombinant Ang2 partially rescues LS-inhibited tubule formation. Collectively, these findings suggest that Ang2 secreted by ECs in response to OS, acts as a promigratory and proangiogenic molecule that could play an important role in diseases with altered shear stress.. Fluid shear stress is thought to play a role in angiogenesis and arteriogenesis. In angiogenesis, endothelial proliferation and migration are 2 important ...

The interaction between fluid status and angiopoietin-2 in adverse renal outcomes of chronic kidney disease. Yi-Chun Tsai, Yi-Wen Chiu, Hung-Tien Kuo, Jia-Jung Lee, Su-Chu Lee, Tzu-Hui Chen, Ming-Yen Lin, Shang-Jyh Hwang, Mei-Chuan Kuo, Ya-Ling Hsu, Hung-Chun Chen. Journal article , Research Article , Published 23 Mar 2017 , PLOS ONE ...

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TY - JOUR. T1 - Angiopoietin-1 regulates brain endothelial permeability through PTPN-2 mediated tyrosine dephosphorylation of occludin. AU - Siddiqui, M. Rizwan. AU - Mayanil, Chandra S.. AU - Kim, Kwang Sik. AU - Tomita, Tadanori. PY - 2015/6/19. Y1 - 2015/6/19. N2 - Objective Blood brain barrier (BBB) breakdown and increased endothelial permeability is a hallmark of neuro-vascular inflammation. Angiopoietin-1 (Ang-1), a Tie-2 receptor agonist ligand, is known to modulate barrier function of endothelial cells; however the molecular mechanisms related to Ang-1 mediated repair of Tight Junctions (TJs) in brain endothelium still remain elusive. In this study, we investigated a novel role of non-receptor protein tyrosine phosphatase N-2 (PTPN-2) in Ang-1 mediated stabilization of tight junction proteins. Method and Result To study the barrier protective mechanism of Ang-1, we challenged human brain microvascular endothelial cells in-vitro, with a potent inflammatory mediator thrombin. By using ...

Angiopoietins are a family of growth factors that are ligands for the tyrosine kinase receptor, Tie2. Angiopoietin 1 (Ang-1) is agonistic for Tie2, plays a key role in blood vessel maturation and stability and has been shown to possess anti-inflammatory properties. However, Tie2 expression has been demonstrated on human neutrophils and the observation that neutrophils migrate in response to Ang-1 in vitro has confounded research into its exact role in inflammation as well as its potential use as a therapeutic agent. We used a mouse model of peritoneal neutrophilic inflammation to determine if Ang-1 could stimulate neutrophil migration in vivo. Tie2 expression was demonstrated on mouse neutrophils. In addition, recombinant human Ang-1 induced significant chemotaxis of isolated mouse neutrophils in a Tie2- and CD18-dependent manner. Subsequently, co-immunoprecipitation of Ang-1 and CD18 demonstrated their interaction. Intraperitoneal injection of an engineered angiopoietin-1, MAT.Ang-1, induced ...

TY - JOUR. T1 - Coadministration of adenoviral vascular endothelial growth factor and angiopoietin-1 enhances vascularization and reduces ventricular remodeling in the infarcted myocardium of type 1 diabetic rats. AU - Mathews Samuel, Samson. AU - Akita, Yuzo. AU - Paul, Debayon. AU - Thirunavukkarasu, Mahesh. AU - Zhan, Lijun. AU - Sudhakaran, Perumana R.. AU - Li, Chuanfu. AU - Maulik, Nilanjana. PY - 2010/1/1. Y1 - 2010/1/1. N2 - OBJECTIVE - Hyperglycemia impairs angiogenesis in response to ischemia, leading to ventricular remodeling. Although the effects of overexpressing angiogenic growth factors have been studied in inducing angiogenesis, the formation of functional vessels remains a challenge. The present study evaluates the reversal of diabetes-mediated impairment of angiogenesis in the infarcted diabetic rat myocardium by proangiogenic gene therapy. RESEARCH DESIGN AND METHODS - Ad.VEGF and Ad.Ang1 were intramyocardially administered in combination immediately after myocardial ...

Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play complementary roles in vascular development during embryogenesis. In the adult, VEGF signaling increases vascular permeability and Ang1 blocks this VEGF-mediated response. Gavard et al. provide evidence that this function of Ang1 is mediated by its actions on the endothelial cells, preventing them from responding to VEGF signaling that triggers disruption of adherens junctions. When injected subcutaneously in mice, Ang1 prevented dermal vascular permeability in response to VEGF injection (acute in vivo vascular permeability model). Experiments with cultured mouse endothelial cells revealed that Ang1 did not prevent VEGF from stimulating downstream effectors involved in proliferation (phosphorylation of Akt, extracellular signal-regulated kinases 1 and 2, and focal adhesion kinase) but did prevent disruption of endothelial barrier function by VEGF. Preexposure of endothelial cell cultures to Ang1 inhibited the ability of ...

TY - JOUR. T1 - Methylglyoxal modification of mSin3A links glycolysis to angiopoietin-2 transcription. AU - Yao, Dachun. AU - Taguchi, Tetsuya. AU - Matsumura, Takeshi. AU - Pestell, Richard. AU - Edelstein, Diane. AU - Giardino, Ida. AU - Suske, Guntram. AU - Ahmed, Naila. AU - Thornalley, Paul J.. AU - Sarthy, Vijay P.. AU - Hammes, Hans Peter. AU - Brownlee, Michael. PY - 2006/1/27. Y1 - 2006/1/27. N2 - Methylglyoxal is a highly reactive dicarbonyl degradation product formed from triose phosphates during glycolysis. Methylglyoxal forms stable adducts primarily with arginine residues of intracellular proteins. The biologic role of this covalent modification in regulating cell function is not known. Here, we report that in retinal Müller cells, increased glycolytic flux causes increased methylglyoxal modification of the corepressor mSin3A. Methylglyoxal modification of mSin3A results in increased recruitment of O-GlcNAc transferase to an mSin3A-Sp3 complex, with consequent increased ...

Angiopoietins and their receptor Tie-2 are, in concert with VEGF-A, key mediators in angiogenesis. This study evaluates the prognostic impact of all known human angiopoietins (Ang-1, Ang-2 and Ang-4) and their receptor Tie-2, as well as their relation to the prognostic expression of VEGF-A. 335 unselected stage I-IIIA NSCLC-patients were included and tissue samples of respective tumor cells and ...

This study demonstrates the distinct spatiotemporal expression profile of Angpt2 after MI and I/R, which, to date, has been obscure due to a lack of reliable antibodies (54). Using our recently developed anti-Angpt2 antibody (23), which is highly sensitive and specific to Angpt2, we demonstrate distinct expressions of Angpt2 on the ECs and proinflammatory macrophages after ischemia. This prompted us to further investigate the role of Angpt2 in postischemic cardiovascular remodeling. We recently reported that the FOXO1/Angpt2 axis suppresses the endothelial PI3K/Akt-signaling pathway, the main downstream pathway for Tie2 that plays a vital role in maintaining vascular integrity, inducing vascular destabilization in a positive feedback manner (25), and that it is central to the pathogenesis of sepsis (23), diabetic retinopathy (25), and tumor vessel destabilization (38). This study provides additional insights into the serious detrimental effects of the FOXO1/Angpt2 axis in exacerbating cardiac ...

Sphingosine-1-phosphate (S1P) is the endogenous ligand for the sphingosine-1-phophate receptors (S1P1-5) and triggers a number of cellular responses through their stimulation. S1P and its interaction with the S1P receptors play a significant role in a variety of biological processes including vascular stabilization, heart development, lymphocyte homing, and cancer angiogenesis. Agonism of S1P1, especially, has been shown to play an important role in lymphocyte trafficking from the thymus and secondary lymphoid organs, inducing immunosuppression, which has been established as a novel mechanism of treatment for immune diseases and vascular diseases. Sphingosine-1 -phosphate (SlP) has been demonstrated to induce many cellular effects, including those that result in platelet aggregation, cell proliferation, cell morphology, tumor cell invasion, endothelial cell and leukocyte chemotaxis, endothelial cell in vitro angiogenesis, and lymphocyte trafficking. SlP receptors are therefore good targets for a ...

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Rabbit polyclonal Angiopoietin 1 + Angiopoietin 2 antibody. Validated in WB, ICC/IF and tested in Rat, Human. Cited in 14 publication(s). Immunogen corresponding to synthetic peptide.

To investigate the theory, Bhandari and colleagues first tested a cohort of lung secretion samples taken from premature infants. After testing the samples, the researchers found that miR-34a was significantly increased only in the samples taken from babies who went on to develop BPD. The researchers also tested lung tissue collected postmortem from babies who had died at different ages from lung disease. Again, they found that this particular microRNA was expressed in the samples and also its downstream targets (the production of proteins) were suppressed in the ones with BPD. Next, the researchers found that levels of miR-34a levels were increased in the lungs of neonatal mice exposed to high levels of oxygen. Bhandari and his team then blocked the microRNA by repressing gene expression, as well as pharmacologically, by injecting the mouse models with the downstream target of the miR-34a, angiopoietin-1. Blocking miR-34a or injecting angiopoietin-1 in the mouse models improved their lung ...

Meet us at the ASBMR in Orlando at The Biomedica booth # 516 Our Posters: #SUN-103, #SUN-271, #MON-252 and #MON-331 Tje Bone Turnover Markers Working Group Room# W307B, Sept 20 at 7:30pm Diurnal Changes in Serum Levels of Bone-Related Circulating MicroRNAs. Oral presentation #1140, room# W314, Monday, Sept 23 from 2:15pm-2:30pm If you cannot make it to the ASBMR ...

The ANGPT-TIE2 pathway is important for angiogenesis, lymphangiogenesis and inflammation, and therefore has important roles in cancer. Given the context-dependent and opposing effects of the ANGPTs, how do we target this pathway? Angiopoietins (ANGPTs) are ligands of the endothelial cell receptor TIE2 and have crucial roles in the tumour angiogenic switch. Increased expression of ANGPT2 relative to ANGPT1 in tumours correlates with poor prognosis. The biological effects of the ANGPT-TIE system are context dependent, which brings into question what the best strategy is to target this pathway. This Review presents an encompassing picture of what we know about this important axis in tumour biology. The various options for

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Polyclonal antibody for Angiopoietin 2/ANGPT2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Angiopoietin 2/ANGPT2 information: Molecular Weight: 56919 MW; Subcellular Localization: Secreted.

Yn aml mae gan enynnau lawer o gyfystyron. Mae hyn oherwydd eu bod yn aml yn cael eu darganfod gan nifer o bobl mewn cyd-destunau gwahanol heb wybod mair un genynnau oeddyn nhw. Hefyd mae gan wahanol gymunedau gwyddonol safonau gwahanol ar gyfer enwi genynnau. Dyma restr o gyfystyron ar gyfer y genyn ANGPT2. ...

Affiliation：名古屋市立大学,大学院医学研究科,講師, Research Field：Ophthalmology, Keywords：脈絡膜新生血管,炎症,加齢黄斑変性,ペリサイト,細胞生物学,リポフスチン,Angiopoietin-2,マウス,発現抑制,脂質, # of Research Projects：5, # of Research Products：33, Ongoing Project：加齢黄斑変性の病態におけるブルッフ膜への加齢性沈着脂質の役割の解明

Angiopoietin 2 antibody (angiopoietin 2) for ELISA, IHC-P, WB. Anti-Angiopoietin 2 pAb (GTX10601) is tested in Human samples. 100% Ab-Assurance.

Angiopoietin 1兔多克隆抗体(ab94684)可与小鼠, 人样本反应并经WB, IHC实验严格验证并得到1个独立的用户反馈。所有产品均提供质保服务，中国75%以上现货。

Published data on the role of the Ang/tie-2 system in cardiovascular diseases are limited. Most of our knowledge regarding the angiopoietins has come from oncology studies where angiogenesis is a prerequisite for tumor growth and metastasis. Indeed, Ang-2 has been shown to be a marker of a poor prognosis in breast cancer and non-small cell lung cancer (4,21). In the present paper, we describe a new assay for Ang-1 and, in addition, have demonstrated (for the first time in the literature) very abnormal levels of Ang-2 and tie-2, but normal Ang-1, in CHF. Vascular endothelial growth factor was marginally raised in the patients, suggesting that Ang-2 may be more relevant to the pathophysiology of this disease. In addition, there was a significant correlation between Ang-2 and tie-2.. That Ang-1 is not raised in CHF is consistent with currently held views that its secretion is not stimulated by hypoxia, as demonstrated in animal studies (13,14,22). Angiopoietin-1 has been shown to have antiapoptotic ...

Background: Microcirculatory dysfunction is associated with multiple organ failure and unfavorable patient outcome. We investigated whether therapeutically targeting the endothelial angiopoietin/Tie2 system preserves microvascular integrity during hemorrhagic shock. Methods: Rats were treated with the angiopoietin-1 mimetic vasculotide and subjected to hemorrhagic shock and fluid resuscitation. Microcirculatory perfusion and leakage were assessed with intravital microscopy (n = 7 per group) and Evans blue dye extravasation (n = 8 per group), respectively. The angiopoietin/Tie2 system was studied at protein and RNA level in plasma, kidneys, and lungs. Results: Hemorrhagic shock significantly reduced continuously perfused capillaries (7 ± 2 vs. 11 ± 2) and increased nonperfused vessels (9 ± 3 vs. 5 ± 2) during hemorrhagic shock, which could not be restored by fluid resuscitation. Hemorrhagic shock increased circulating angiopoietin-2 and soluble Tie2 significantly, which associated with ...

Angiopoietin-related protein 2 also known as angiopoietin-like protein 2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietin-like protein 2 maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue reconstruction, whereas an excess of ANGPTL2 activation induced by prolonged stress promotes the breakdown of tissue homeostasis due to chronic inflammation, promoting the development of metabolic diseases. ANGPTL2 has a role also in angiogenesis, in tissue repair, in obesity, in atherosclerotic diseases and finally in cancerogenesis. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. GRCh38: ...

Notch and Angiopoietin-1 (Ang1)/Tie2 pathways are crucial for vascular maturation and stability. Here we identify the transcription factor ERG as a key regulator of endothelial Notch signalling. We show that ERG controls the balance between Notch ligands by driving Delta-like ligand 4 (Dll4) while repressing Jagged1 (Jag1) expression. In vivo, this regulation occurs selectively in the maturing plexus of the mouse developing retina, where Ang1/Tie2 signalling is active. We find that ERG mediates Ang1-dependent regulation of Notch ligands and is required for the stabilizing effects of Ang1 in vivo. We show that Ang1 induces ERG phosphorylation in a phosphoinositide 3-kinase (PI3K)/Akt-dependent manner, resulting in ERG enrichment at Dll4 promoter and multiple enhancers. Finally, we demonstrate that ERG directly interacts with Notch intracellular domain (NICD) and {beta}-catenin and is required for Ang1-dependent {beta}-catenin recruitment at the Dll4 locus. We propose that ERG coordinates Ang1, ...

Background. In sepsis and various other inflammatory conditions, elevated circulating levels of angiopoietin-2 (Ang2) are detected, but the precise functional role of Ang2 in these conditions is not well understood. Here, we investigated the contribution of Ang2 to the inflammatory response and renal function impairment in a mouse model of endotoxaemia.. Methods. Ang2-deficient mice and wild-type littermates were challenged with lipopolysaccharide [LPS; 1500 EU/g, intraperitoneal (i.p.)]. In additional experiments, wild-type C57Bl/6 mice were depleted of circulating neutrophils by antibody treatment (NIMPR14) prior to LPS challenge to study the role of neutrophils in regulating LPS-induced cytokine release. After 8 or 24 h of LPS challenge, the mice were sacrificed and organs were harvested. Quantitative reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay were performed for endothelial adhesion molecules (P-selectin, E-selectin, VCAM-1 and ICAM-1) and plasma ...

Hyperoxia-induced acute lung injury (HALI) is a key contributor to the pathogenesis of bronchopulmonary dysplasia (BPD) in neonates, for which no specific preventive or therapeutic agent is available. Here we show that lung micro-RNA (miR)-34a levels are significantly increased in lungs of neonatal mice exposed to hyperoxia. Deletion or inhibition of miR-34a improves the pulmonary phenotype and BPD-associated pulmonary arterial hypertension (PAH) in BPD mouse models, which, conversely, is worsened by miR-34a overexpression. Administration of angiopoietin-1, which is one of the downstream targets of miR34a, is able to ameliorate the BPD pulmonary and PAH phenotypes. Using three independent cohorts of human samples, we show that miR-34a expression is increased in type 2 alveolar epithelial cells in neonates with respiratory distress syndrome and BPD. Our data suggest that pharmacologic miR-34a inhibition may be a therapeutic option to prevent or ameliorate HALI/BPD in neonates.

Mice. Specific pathogen-free (SPF) C57BL/6J mice (catalog 000664), Tie2-GFP mice (catalog 003658), and UBC-Cre-ERT2 mice (catalog 007001) were purchased from the Jackson Laboratory. Angpt1fl/fl mice were a gift from Yoshikazu Nakaoka (Osaka University, Osaka, Japan), Angpt1-GFP mice were a gift from Sean J. Morrison (University of Texas Southwestern, Dallas, Texas, USA), Angpt2-lacZ mice were a gift from Nicholas Gale (Regeneron Pharmaceuticals), and Vegfr2fl/fl mice were a gift from Masanori Hirashima (Kobe University, Kobe, Japan). Prox1-GFP (30), Angpt1-GFP (33), Prox1fl/fl (39), Tie2fl/fl (35), Angpt1fl/fl (36, 37), Angpt2fl/fl (38), Vegfr2fl/fl (55), and Angpt2-lacZ (22) mice were transferred and bred in our SPF animal facility at KAIST. To deplete Prox1, Tie2, or Vegfr2 genes specifically in ECs, VE-cadherin-Cre-ERT2 mice (34) were mated with either Prox1fl/fl, Tie2fl/fl, or Vegfr2fl/fl mice to obtain EC-specific Prox1-, Tie2-, or Vegfr2-deleted mice, respectively, in a tamoxifen-dependent ...

Human platelet lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides.. ...

ENCODES a protein that exhibits identical protein binding (ortholog); INVOLVED IN animal organ regeneration (ortholog); liver regeneration (ortholog); negative regulation of apoptotic process (ortholog); PARTICIPATES IN angiopoietin signaling pathway; ASSOCIATED WITH brain ischemia (ortholog); Diabetes Mellitus, Experimental (ortholog); diabetic retinopathy (ortholog); FOUND IN extracellular space (inferred); integral component of membrane (inferred); membrane raft (inferred)

Purified Recombinant Human ANGPT1/COMP protein, FLAG-tagged from Creative Biomart. Recombinant Human ANGPT1/COMP protein, FLAG-tagged can be used for research.

401366DNAHomo sapiens 1gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggggggtc cctgagactc 60tcctgtgcag tctctggatt cacctttagt agctattgga tgagctgggt ccgccaggct 120ccagggaagg ggctggagtg ggtggccaac ataaagcaag atggaagtga gaaatactat 180gtggactctg tgaagggccg attcaccatc tccagagaca acgccaagaa ctcactgtat 240ctgcaaatga acagcctgag agccgaggac acggctgtgt attactgtgc gagagatcaa 300ggtatagcag tggctgggcc ctttgactac tggggccagg gaaccctggt caccgtctcc 360tcagcc 3662122PRTHomo Sapiens 2Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly1 5 10 15Ser Leu Arg Leu Ser Cys Ala Val Ser Gly Phe Thr Phe Ser Ser Tyr 20 25 30Trp Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 35 40 45Ala Asn Ile Lys Gln Asp Gly Ser Glu Lys Tyr Tyr Val Asp Ser Val 50 55 60Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr65 70 75 80Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 85 90 95Ala Arg Asp Gln Gly Ile Ala Val Ala Gly Pro Phe Asp Tyr Trp Gly 100 105 110Gln Gly Thr Leu Val Thr Val Ser ...

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Explore the angiopoietin-TIE2 signaling pathway and find antibodies to detect some of its target proteins, including CD202b (TIE2), AKT, ERK1, and ERK2.

ANGPT2 (Human) ELISA Kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ANGPT2. (KA1867) - Products - Abnova

Detect and quantitate human angiopoietin 1 (ANGPT1) in buffered solution and cell culture supernatants using a homogeneous LANCE Ultra TR-FRET assay.

Camenisch G et al. (2002) ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha vbeta 3 and induces blood vessel formation in vivo.. [^] ...

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