OLIVEIRA, Eder Jorge de et al. Molecular characterization of papaya genotypes using AFLP markers. Rev. Bras. Frutic. [online]. 2011, vol.33, n.3, pp.849-858. ISSN 0100-2945. https://doi.org/10.1590/S0100-29452011000300020.. Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar Sunrise and the inbred line CMF-L30-08 were the closest genotypes, and the improved ...
Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient method for producing DNA fingerprints and molecular characterization. Our objectives were to: estimate genetic similarities (GS), marker indices, and polymorphic information contents (PICs) for AFLP markers in almond cultivars; assess the genetic diversity of almond cultivars and wild species, using GS estimated from AFLP fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression and cultivar improvement. The genetic diversity of 45 almond cultivars from Iran, Europe, and America, were studied assaying 19 primer combinations. In addition, several agronomic traits were evaluated, including flowering and maturity times, self-incompatibility, and kernel and fruit properties. Out of the 813 polymerase chain reaction fragments that were scored, 781 (96.23%) were polymorphic. GS ranged from 0.5 to 0.96, marker indices ranged from 51.37 to 78.79, and PICs ranged from 0.56 ...
Principal coordinate analysis (PCoA) plot of samples from ground and flight mice.Flight mice data points clustered relatively tightly within the bottom right qu
TY - JOUR. T1 - Identification Of AFLP markers associated with round heart syndrome in Turkeys. AU - Paxton, C. N.. AU - Pierpont, M. E.. AU - Kooyman, D. L.. PY - 2005. Y1 - 2005. N2 - The Amplified Fragment Length Polymorphism (AFLP) method was used to identify 38 genetic markers that associate with either phenotypically normal or round heart turkeys, diagnosed by gross necropsy, from an inbred flock of Nicholas broad-breasted birds. Sixty-five polymorphisms were identified and analyzed by chi-square. A p-value of less than 0.05 determined markers that were kept and sequenced. Thirty-eight markers were identified, 19 that associated with the normal phenotype and 19 that associated with the round heart syndrome. The majority of the markers had a high A/T content, suggesting possible regulatory domains in which polymorphisms were occurring. Sequence Characterized Amplified Region (SCAR) primers were designed from marker sequences with the hopes of designing a simple Marker Assisted Selection ...
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Dive into the research topics of Infrared, Raman and temperature-dependent NMR spectra, vibrational assignments, normal coordinate analysis, and DFT calculations of benzoxazoline-2-thione. Together they form a unique fingerprint. ...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
PCOORD (Principal Coordinate Analysis) is a procedure to find meaningful patterns in sequence data with no a priori knowledge about them. The procedure attempts to summarize the variation in the sequences in a limited number of axes or dimensions. A dimension is basically a combination of positions in a sequence that behave similarly (for example, position 133 usually has an A when position 250 has a G). One way to describe the process of finding these dimensions is as follows. If we have a two-dimensional swarm of data points, then we need two dimensions (the X and Y axis) to describe the variation in our data. However, if the swarm is very elongated and the points almost lie on a straight line, then we really need only one dimension, although we use two. PCOORD uses a mathematical method to find the best way to describe a multi-dimensional dataset in a smaller number of dimensions, which are linear combinations of the original dimensions. The dimensions are not necessarily biologically ...
Dussle CM, Quint M, Xu ML, Melchinger AE, Lübberstedt T (2003) Saturation of two chromosome regions conferring resistance to SCMV with SSR and AFLP markers by targeted BSA. [http://www.ncbi.nlm.nih.gov/pubmed/12589549?ordinalpos=10&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum Theoretical and Applied Genetics 106: 485-493].,br ...
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AFLP protocol using IRDye infrared fluorescent dyes for large plant genome analysis. Manuals for 4300 DNA Analyzer and 4300 DNA Analysis system Brochure
READ THIS Farewell Letter from a Blogger who was forced to shut down his site... Well as you know all good things must come to an end. Tod ...
Where do I start with this class? I basically waited for a little over two years for Larry Vickers to come back down to my area with a class that would aligned with my schedule. I think ever since the Alias Training company fiasco, a lot of my training plans were out the window. But […]. ...
TY - JOUR. T1 - Evaluation of genetic diversity in Omani banana cultivars (Musa cvs.) using AFLP markers. AU - Al-Saady, Nadiya A.. AU - Al-Lawati, Abbas H.. AU - Al-Subhi, Ali M.. AU - Khan, Akhtar J.. PY - 2010. Y1 - 2010. N2 - The aim of the present study was to investigate the genetic diversity among eighteen banana cultivars collected from Al-Batinah, Al-Dhakhliya and Dhofar regions of the Sultanate of Oman using AFLP markers. Eleven AFLP primer combinations were used to develop banana DNA fingerprints. Unweighted Pair Group Method with Arithmetic mean (UPGMA) cluster analysis yielded three distinct taxa. Banana cultivars, Bahri, Omani, Maisori Fardh, Sokari and Zanzibar from Al-Dhakhliya region grouped in cluster 1, whereas cultivars from Dhofar, Dwarf spotted Cavendish, Somali, Abubaker Philipino, Maisori Fardh, Milk Banana, Plantain Kenya and Sawara Red grouped in cluster 2 and Williams, Somali, Malindi, Red Banana, Maisori Fardh and Nagal cultivars from Al-Batinah region grouped in ...
  Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. (sens. lat.) into three distinct genera namely, Chamaecrista Moench.,Senna P. Mill. and Cassia L. (sens.str.).  Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci), respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using the SHAN clustering, divided Cassia L. (sens. lat.) into three different clusters as Chamaecrista Moench., Senna P. Mill. and Cassia L. (sens.str.). High bootstrap value revealed that all
found. Phylogenetic trees among 5 plant varieties were constructed based on Neis coefficient standard genetic distances using unweighted pair group method with arithmetic mean (UPGMA) method. For RAPD and AFLP analysis, Gujarat Methi-1 (GM-1) and Gujarat Methi-2 (GM-2) clustered together showed more similarities than other varieties. In fenugreek, RAPD markers were found more polymorphic than AFLP markers. RAPD markers also showed more diversity in comparison to AFLP markers, although the data generated for AFLP markers were more authenticated and reproducible than RAPD markers.. Key words: AFLP, RAPD, genetic diversity, automated genetic analyzer, polymorphism.. ...
Cotton is the worlds primary fiber crop and is a major agricultural commodity in over 30 countries. Like many other global commodities, sustainable cotton production is challenged by restricted natural resources. In response to the anticipated increase of agricultural water demand, a major research direction involves developing crops that use less water or that use water more efficiently. In this study, our objective was to identify differentially expressed genes in response to water deficit stress in cotton. A global expression analysis using cDNA-Amplified Fragment Length Polymorphism was conducted to compare root and leaf gene expression profiles from a putative drought resistant cotton cultivar grown under water deficit stressed and well watered field conditions. We identified a total of 519 differentially expressed transcript derived fragments. Of these, 147 transcript derived fragment sequences were functionally annotated according to their gene ontology. Nearly 70 percent of transcript derived
If you have ever seen a jackfruit, you will know that it is a massive fruit. Usually found at specialty or Asian grocery stores, jackfruits are the largest tree-borne fruits in the world. Each jackfruit can weight between 6-40 pounds. Some people get jackfruit and durian mixed up, but the jackfruit is much more palatable and grows much bigger. It has also been known to be a powerful cancer killer.. The jackfruit has lots of seeds inside, but the fruit is mainly composed of healthy starch and protein. In addition to being rich in healthy flavonoids, jackfruit has plenty of vitamin C, which is a powerful antioxidant that nourishes cells in the body. Each bulb of the fruit, which is made of sweet yellow flesh, is replete with a group of B-complex vitamins.. How Does Jackfruit Kill Cancer Cells?. As mentioned in the paragraph above, jackfruit contains a lot essential nutrients that benefit the entire body. Additionally, jackfruit has saponins, isoflavones, and lignans, which are phytonutrients that ...
Genetic Diversity in Musa acuminata Colla and Musa balbisiana Colla and some of their natural hybrids using AFLP Markers.. PubMed. Ude, G.; Pillay, M.; Nwakanma, D.; Tenkouano, A.. 2002-06-01. Genetic diversity and relationships were assessed in 28 accessions of Musa acuminata (AA) Colla and Musa balbisiana (BB) Colla, and some of their natural hybrids, using the amplified fragment length polymorphisms (AFLP) technique. Fifteen AFLP +3 primer pairs produced 527 polymorphic bands among the accessions. Neighbor-joining and principal co-ordinate (PCO) analyses using Jaccards similarity coefficient produced four major clusters that closely corresponded with the genome composition of the accessions (AA, BB, AAB and ABB). The AFLP data distinguished between the wild diploid accessions and suggested new subspecies relationships in the M. acuminata complex that are different from those based on morphological data. The data suggested that there are three subspecies within the M. acuminata complex (ssp. ...
Genetic diversity within species may promote resilience to environmental change, yet little is known about how such variation is distributed at broad geographic scales. Here we develop a novel Bayesian methodology to analyse multi-species genetic diversity data in order to identify regions of high or low genetic diversity. We apply this method to co-distributed taxa from Australian marine waters. We extracted published summary statistics of population genetic diversity from 118 studies of 101 species and | 1000 populations from the Australian marine economic zone. We analysed these data using two approaches: a linear mixed model for standardised data, and a mixed beta-regression for unstandardised data, within a Bayesian framework. Our beta-regression approach performed better than models using standardised data, based on posterior predictive tests. The best model included region (Integrated Marine and Coastal Regionalisation of Australia (IMCRA) bioregions), latitude and latitude squared. Removing
Jackfruit is an aromatic, fleshy fruit celebrated for its delicious taste. But many people who enjoy eating jackfruit do so finally unaware of its many health benefits. Jackfruit is a tree species belong to the mulberry family. Jackfruit is very sweet and tasty, while the jackfruit is opened, you will find the bright yellow pods (when ripe) which can be eaten raw or cooked. When immature its flesh is green, and it can be made into a delicious vegetable dish. Jackfruit has a lot of health benefits ...
Genomic DNA was extracted from 25 mg of tissue, collected from 67 C. taurus from the eastern and western Australian coasts and South Africa (figure 1), using a QIAmp Tissue Kit (Qiagen Inc., Valencia, CA). AFLP typing of 65 individuals (table 1) involved digestion of 200-400 ng of genomic DNA with the restriction enzymes MseI and EcoRI and generation of profiles using 12 different primer pairs in the final (selective) polymerase chain reaction (PCR). Each primer pair combination had a total of three selective nucleotides on the EcoRI primer (GAC TGC GTA CCA ATT C+ACT, AGT, ATC or AAC) and four selective nucleotides on the MseI primer (GAT GAG TCC TGA GTA A+CAAC, CTGC, CAGC or CTTC). A total of 235 polymorphic AFLP loci were scored. Allele frequencies were estimated with a Bayesian method, assuming a non-uniform prior distribution of allele frequencies (AFLP-SURV v. 1.0; Vekemans 2002). The same software calculated expected heterozygosity (He), pairwise Fst (significance assessed by 1000 ...
Jackfruit is a popular fruit in India though it is not abundantly available in all parts of India. Jackfruit is widely grown in Kerala. You can find Jackfruit trees in almost all parts of Kerala. As it is abundantly found in Kerala people do not...
Amplified fragment length polymorphism (AFLP™) is a PCR based DNA fingerprinting technique that generates band profiles via the selective amplification of restriction fragments of whole genomic DNA. The method can be used both for identification and typing.
Medicinal advantages of jackfruit nutrition are improving glucose tolerance in both type-2 and normal diabetes patients. Nutrients present are dietary fiber and 155 calories.
Top ⭐ 70 reasons for Jackfruit: 1. More proteins per 100g: 1.72 2. Less cholesterol per 100g: 0 3. Smaller amount of sugars per 100g: 19.08 4. More lipids per 100g: 0.64
Nutrition Details For 1 Cup, Sliced (165g) Of Jackfruit Raw - Get a bar chart of the top 10 nutrients, and click to see an expanded list of over 151 nutrients, including amino acids.
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Field resistance to cyst nematode (SCN) race 3 (Heterodera glycines I.) in soybean [Glycine max (L.) Merr.] cv Forrest is conditioned by two QTLs: the underlying genes are presumed to include Rhg1 on linkage group G and Rhg4 on linkage group A2. A population of recombinant inbred lines (RILs) and two populations of near-isogenic lines (NILs) derived from a cross of Forrest × Essex were used to map the loci affecting resistance to SCN. Bulked segregant analysis, with 512 AFLP primer combinations and microsatellite markers, produced a high-density genetic map for the intervals carrying Rhg1 and Rhg4. The two QTLs involved in resistance to SCN were strongly associated with the AFLP marker EATGMCGA87 (P = 0.0001, R2 = 24.5%) on linkage group G, and the AFLP marker ECCGMAAC405 (P = 0.0001, R2 = 26.2%) on linkage group A2. Two-way analysis of variance showed epistasic interaction (P = 0.0001, R2 =16%) between the two loci controlling SCN resistance in Essex × Forrest recombinant inbred lines. Considering
TY - JOUR. T1 - Amplified fragment length polymorphism based identification of genetic markers and novel PCR assay for differentiation of Campylobacter fetus subspecies. AU - van Bergen, M.A.P.. AU - Simons, G.. AU - van der Graaf-van Bloois, L.. AU - van Putten, J.P.. AU - Rombout, J.. AU - Wesley, I.. AU - Wagenaar, J.A.. PY - 2005. Y1 - 2005. N2 - Differentiation of Campylobacter fetus into C. fetus subsp. fetus (Cff) and C. fetus subsp. venerealis (Cfv) is important for both clinical and economic reasons. In the past, several molecular typing methods have been used for differentiation, including amplified fragment length polymorphism (AFLP). In this study, AFLP was employed to identify C. fetus subspecies specific markers that can serve as a basis for design of novel PCR primer sets for Cfv. Four groups of C. fetus strains with different phenotypic or genotypic traits were examined by AFLP using 22 different DdeI/MboI primer combinations. Specific AFLP fragments were deduced and sequenced ...
Three Iranian native strains and three Japanese commercial lines of the silkworm Bombyx mori were analyzed using amplified fragment length polymorphism (AFLP) markers. A set of ten PstI/TaqI primer combinations amplified a total of 322 bands out of which 251 (%78) were polymorphic. Estimates of Neis gene diversity for all loci in individual strains and commercial lines indicated a higher degree of genetic similarity within Japanese commercial lines than the Iranian native strains. The highest and the least degree of gene diversity were related to the Khorasan Orange strain (h=0.1812) and P107 commercial line (h=0.0804), respectively. The dendrogran constructed using the unweighted pair-group method with arithmitic average based on nais genetic distance revealed two distinct groups as Khorasan native and Japanese commercial lines. The distinct clustering of these two sets of strains and lines reflects differences of the geographical origin and morphological, qualitative and quantitative traits
Abstract: The AFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes (1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified with a LOD score =6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accumulation. Two framework maps of the same F1 ...
This study aimed at investigating the genetic diversity of a panel of Candida africana strains recovered from vaginal samples in different countries. All fungal strains were heterozygous at the mating type-like locus and belonged to the genotype A of Candida albicans. Moreover, all examined C. africana strains lack N-acetylglucosamine assimilation and sequence analysis of the HXK1 gene showed a distinctive polymorphism that impair the utilization of this aminosugar in this yeast.Multilocus sequencing of seven housekeeping genes revealed a substantial genetic homogeneity among the strains, except for the CaMPIb and VPS13 loci which contributed significantly to the classification of our set of C. africana strains into 6 existing diploid sequence types. Amplified fragment length polymorphism (AFLP) fingerprint analysis yielded greater genotypic heterogeneity among the C. africana strains. Overall the data reported here show that in C. africana genetic diversity occurs and the existence of this intriguing
Synonyms for restriction fragment length polymorphism at Thesaurus.com with free online thesaurus, antonyms, and definitions. Dictionary and Word of the Day.
Anderson, G. J.; G. Bernardello, T. F. Stuessy & D. J. Crawford. 2001. Breeding system and pollination of selected plants endemic to Juan Fernández Islands. American Journal of Botany 88: 220-233. Arrigo, N.; J. W. Tuszynski, D. Ehrich, T. Gerdes & N. Álvarez. 2009. Evaluating the impact of scoring parameters on the structure of intra-specific genetic variation using RawGeno, an R package for automating AFLP scoring. BMC Bioinformatics 10: 33. de Queiroz, K. 2007. Species concepts and species delimitation. Systematic Biology 56: 879-886. Doyle, J. J. & J. L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11-15. Ehrich, D. 2006. AFLPDAT: a collection of R functions for convenient handling of AFLP data. Molecular Ecology Notes 6: 603-604. Excoffier, L. & H. E. L. Lischer. 2010. Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources 10: ...
Interpretive Summary: One of the top-selling international medicinal products is Hypericum perforatum (St. Johns Wort). Despite its worldwide distribution and utilization, little is known regarding the relationship of the bioactive compounds in H. perforatum to the plants from which they are harvested. In this study, Amplified Fragment Length Polymorphism (AFLP) analysis of 56 Hypericum accessions, representing 11 species, was conducted to gain a better understanding of diversity within Hypericum species, especially within cultivated accessions of H. perforatum, and to establish a molecular method that will provide breeders and regulators with a simple, affordable, and accurate tool with which to identify H. perforatum material in commercial products. AFLP analysis is a whole-genome approach that has broad applicability in determining genetic variability within and among plant populations, crop origins, and relationships among cultivars. AFLP markers are highly repeatable, provide broad genomic ...
The development of sugarcane as a sustainable crop has unlimited applications. The crop is one of the most economically viable for renewable energy production, and CO2 balance. Linkage maps are valuable tools for understanding genetic and genomic organization, particularly in sugarcane due to its complex polyploid genome of multispecific origins. The overall objective of our study was to construct a novel sugarcane linkage map, compiling AFLP and EST-SSR markers, and to generate data on the distribution of markers anchored to sequences of scIvana_1, a complete sugarcane transposable element, and member of the Copia superfamily. The mapping population parents (IAC66-6 and TUC71-7) contributed equally to polymorphisms, independent of marker type, and generated markers that were distributed into nearly the same number of co-segregation groups (or CGs). Bi-parentally inherited alleles provided the integration of 19 CGs. The marker number per CG ranged from two to 39. The total map length was 4,843.19 cM
RFLP (Restriction Fragment Length Polymorphism) RFLP RFLP was developed at the late 70 s due to the discovery of restriction enzymes (REs; or called as restriction ... – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 425ce8-OTc2N
Jackfruit, a tropical fruit with a dense, chewy texture, is a blank canvas that takes on flavors well. In these vegan burrito bowls, the jackfruit is simmered in a warm and spicy chile sauce thats so good youll never know youre eating a plant-based protein instead of pork or beef.
This tutorial illustrates how to calculate a Principal Components Analysis (PCA) and a Multi Dimensional Scaling (MDS) (sometimes also called Principal Coordinates Analysis (PCoA)) on a fingerprint data set and how to change the layout of the obtained plots.
all of the following can be used to detect differences in DNA among individuals have been ... are produced through the use of restriction enzymes
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Lol , Agreement requires a blood sample , eye retina scan , Restriction Fragment Length Polymorphism , Mitochondrial and Y-Chromosome comparisons to your mother , father , children and grand parents and last but not least your soul. Read full review ...
Microsatellite (STR, SSR) analysis by Fragment Length Analysis (FLA) ** More info? +49 8092 8289-0 ** Please call to talk to one of our experienced staff.
Fomblin Perfluorosolv PFS-1 Solvent is a low molecular weight perfluoropolyether which can be used as a CFC113 replacement in many industrial applications.
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[IMG].[IMG][IMG] Brooke Shields prepares to board the private jet in Burbank [IMG] With Tom Cruise and Katie Holmess nuptials merely days...
Of course, this doesnt particularly change a lot. There are still a lot of unvaccinated people out there (including everyone 15 and younger), so I cant just pretend everything is okay. A young(er that 5) kid died in my county this week, so I dont foresee myself taking the kids out to lick light poles yet. But maybe Ill browse a store for a little bit ...
We implemented one of our product purely on top Cassandra back in March 2010 with version 0.5.1. It went live in May, and tanked in the first week due to crazy traffic. Three painful, sleepless weeks later, Cassandra finally dropped its pants and started corrupting our data. We shutdown the site, spent three full days,…
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