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The present study was conducted to evaluate alternative methods to prolong the persistence of ampicillin in the horse. Artificial alkalinisation (pH , 7.91 +/- 0.13) and acidification (pH , 4.24 +/- 0.13) of equine urinary pH achieved by intragastric administration of sodium bicarbonate (100, 200, 300, and 400 [x3] mg/kg) over a six day period, and ammonium chloride (200, 300, and 400 [x3] mg/kg) over a five day period respectively, did not produce any significant changes in the plasma disposition of ampicillin when administered as an IV bolus (10 mg/kg) of the sodium salt to horses. These results were attributed to the lack of changes in the extent of reabsorption of ampicillin from the urine into plasma caused by the zwitterion characteristics of the antibiotic. However, the alkalinisation of urine appeared to affect the fraction of the dose of ampicillin recovered in the urine since only a small fraction could be measured, (,15 %) presumably resulting from a degradation of the antibiotic in ...
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